LOCUS       DAZKAS010000001        68354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000001 DAZKAS010000000
VERSION     DAZKAS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.001"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..909
                     /locus_tag="ACG8H7_000001"
     CDS             28..909
                     /locus_tag="ACG8H7_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8982147.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="ACG8H7_000002"
     CDS             922..2532
                     /locus_tag="ACG8H7_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982148.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="ACG8H7_000003"
     CDS             complement(2609..3268)
                     /locus_tag="ACG8H7_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP8982149.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="ACG8H7_000004"
     CDS             complement(3551..4216)
                     /locus_tag="ACG8H7_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFP8982150.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8H7_000005"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="ACG8H7_000005"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP8982151.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8H7_000006"
     CDS             complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="ACG8H7_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP8982152.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8H7_000007"
     CDS             complement(5981..6376)
                     /gene="dhaM"
                     /locus_tag="ACG8H7_000007"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP8982153.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="ACG8H7_000008"
     CDS             6530..6850
                     /locus_tag="ACG8H7_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP8982154.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7819)
                     /locus_tag="ACG8H7_000009"
     CDS             complement(6962..7819)
                     /locus_tag="ACG8H7_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP8982155.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7927..9230)
                     /locus_tag="ACG8H7_000010"
                     /pseudo
     CDS             complement(7927..9230)
                     /locus_tag="ACG8H7_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9227..9736)
                     /locus_tag="ACG8H7_000011"
     CDS             complement(9227..9736)
                     /locus_tag="ACG8H7_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982156.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9911..12046)
                     /locus_tag="ACG8H7_000012"
     CDS             complement(9911..12046)
                     /locus_tag="ACG8H7_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP8982157.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12129..13784)
                     /locus_tag="ACG8H7_000013"
     CDS             complement(12129..13784)
                     /locus_tag="ACG8H7_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8982158.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13786..14100)
                     /locus_tag="ACG8H7_000014"
     CDS             complement(13786..14100)
                     /locus_tag="ACG8H7_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP8982159.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14270..14530
                     /locus_tag="ACG8H7_000015"
     CDS             14270..14530
                     /locus_tag="ACG8H7_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFP8982160.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14544..14858
                     /locus_tag="ACG8H7_000016"
     CDS             14544..14858
                     /locus_tag="ACG8H7_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFP8982161.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15588..16142)
                     /locus_tag="ACG8H7_000017"
     CDS             complement(15588..16142)
                     /locus_tag="ACG8H7_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP8982162.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16268..18196)
                     /locus_tag="ACG8H7_000018"
     CDS             complement(16268..18196)
                     /locus_tag="ACG8H7_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP8982163.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18502..19218)
                     /locus_tag="ACG8H7_000019"
     CDS             complement(18502..19218)
                     /locus_tag="ACG8H7_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP8982164.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19219..21042)
                     /gene="glpO"
                     /locus_tag="ACG8H7_000020"
     CDS             complement(19219..21042)
                     /gene="glpO"
                     /locus_tag="ACG8H7_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFP8982165.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21056..22552)
                     /gene="glpK"
                     /locus_tag="ACG8H7_000021"
     CDS             complement(21056..22552)
                     /gene="glpK"
                     /locus_tag="ACG8H7_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP8982166.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22688..24148)
                     /locus_tag="ACG8H7_000022"
     CDS             complement(22688..24148)
                     /locus_tag="ACG8H7_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982167.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24138..25433)
                     /locus_tag="ACG8H7_000023"
     CDS             complement(24138..25433)
                     /locus_tag="ACG8H7_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8982168.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25765..26442)
                     /locus_tag="ACG8H7_000024"
     CDS             complement(25765..26442)
                     /locus_tag="ACG8H7_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFP8982169.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26472..27563)
                     /locus_tag="ACG8H7_000025"
     CDS             complement(26472..27563)
                     /locus_tag="ACG8H7_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP8982170.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27664..28098)
                     /locus_tag="ACG8H7_000026"
     CDS             complement(27664..28098)
                     /locus_tag="ACG8H7_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP8982171.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28682..30139)
                     /locus_tag="ACG8H7_000027"
     CDS             complement(28682..30139)
                     /locus_tag="ACG8H7_000027"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8982172.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30136..32031)
                     /locus_tag="ACG8H7_000028"
     CDS             complement(30136..32031)
                     /locus_tag="ACG8H7_000028"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP8982173.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32089..33864)
                     /locus_tag="ACG8H7_000029"
     CDS             complement(32089..33864)
                     /locus_tag="ACG8H7_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP8982174.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33914..35410)
                     /locus_tag="ACG8H7_000030"
     CDS             complement(33914..35410)
                     /locus_tag="ACG8H7_000030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8982175.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35604..37226)
                     /locus_tag="ACG8H7_000031"
     CDS             complement(35604..37226)
                     /locus_tag="ACG8H7_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP8982176.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37675..38988)
                     /locus_tag="ACG8H7_000032"
     CDS             complement(37675..38988)
                     /locus_tag="ACG8H7_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP8982177.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39233..39457)
                     /locus_tag="ACG8H7_000033"
     CDS             complement(39233..39457)
                     /locus_tag="ACG8H7_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982178.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39477..39899)
                     /locus_tag="ACG8H7_000034"
     CDS             complement(39477..39899)
                     /locus_tag="ACG8H7_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8982179.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40043..40753)
                     /locus_tag="ACG8H7_000035"
     CDS             complement(40043..40753)
                     /locus_tag="ACG8H7_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFP8982180.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40771..41268)
                     /locus_tag="ACG8H7_000036"
     CDS             complement(40771..41268)
                     /locus_tag="ACG8H7_000036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8982181.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41305..41757)
                     /locus_tag="ACG8H7_000037"
     CDS             complement(41305..41757)
                     /locus_tag="ACG8H7_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8982182.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41892..42497)
                     /locus_tag="ACG8H7_000038"
     CDS             complement(41892..42497)
                     /locus_tag="ACG8H7_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP8982183.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42947..43567)
                     /locus_tag="ACG8H7_000039"
     CDS             complement(42947..43567)
                     /locus_tag="ACG8H7_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8982184.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43569..44438)
                     /locus_tag="ACG8H7_000040"
     CDS             complement(43569..44438)
                     /locus_tag="ACG8H7_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP8982185.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44461..44868)
                     /locus_tag="ACG8H7_000041"
     CDS             complement(44461..44868)
                     /locus_tag="ACG8H7_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP8982186.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44887..46176)
                     /locus_tag="ACG8H7_000042"
     CDS             complement(44887..46176)
                     /locus_tag="ACG8H7_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8982187.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46203..46505)
                     /locus_tag="ACG8H7_000043"
     CDS             complement(46203..46505)
                     /locus_tag="ACG8H7_000043"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8982188.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46530..48623)
                     /locus_tag="ACG8H7_000044"
     CDS             complement(46530..48623)
                     /locus_tag="ACG8H7_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8982189.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49112..49876)
                     /locus_tag="ACG8H7_000045"
     CDS             complement(49112..49876)
                     /locus_tag="ACG8H7_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFP8982190.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49987..50856)
                     /gene="mdcB"
                     /locus_tag="ACG8H7_000046"
     CDS             complement(49987..50856)
                     /gene="mdcB"
                     /locus_tag="ACG8H7_000046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFP8982191.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50881..51957)
                     /locus_tag="ACG8H7_000047"
     CDS             complement(50881..51957)
                     /locus_tag="ACG8H7_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP8982192.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51981..52574)
                     /locus_tag="ACG8H7_000048"
     CDS             complement(51981..52574)
                     /locus_tag="ACG8H7_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFP8982193.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52578..54254)
                     /gene="mdcD"
                     /locus_tag="ACG8H7_000049"
     CDS             complement(52578..54254)
                     /gene="mdcD"
                     /locus_tag="ACG8H7_000049"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFP8982194.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54244..54543)
                     /locus_tag="ACG8H7_000050"
     CDS             complement(54244..54543)
                     /locus_tag="ACG8H7_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFP8982195.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54645..56291)
                     /gene="mdcA"
                     /locus_tag="ACG8H7_000051"
     CDS             complement(54645..56291)
                     /gene="mdcA"
                     /locus_tag="ACG8H7_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFP8982196.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56298..57209)
                     /locus_tag="ACG8H7_000052"
     CDS             complement(56298..57209)
                     /locus_tag="ACG8H7_000052"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP8982197.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57329..58186
                     /locus_tag="ACG8H7_000053"
     CDS             57329..58186
                     /locus_tag="ACG8H7_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8982198.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58373..58735)
                     /locus_tag="ACG8H7_000054"
     CDS             complement(58373..58735)
                     /locus_tag="ACG8H7_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP8982199.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58924..59106)
                     /locus_tag="ACG8H7_000055"
     CDS             complement(58924..59106)
                     /locus_tag="ACG8H7_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982200.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59081..60520
                     /locus_tag="ACG8H7_000056"
     CDS             59081..60520
                     /locus_tag="ACG8H7_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982201.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60527..63079)
                     /locus_tag="ACG8H7_000057"
     CDS             complement(60527..63079)
                     /locus_tag="ACG8H7_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFP8982202.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63193..64524)
                     /locus_tag="ACG8H7_000058"
     CDS             complement(63193..64524)
                     /locus_tag="ACG8H7_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8982203.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64490..65968)
                     /locus_tag="ACG8H7_000059"
     CDS             complement(64490..65968)
                     /locus_tag="ACG8H7_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8982204.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66273..68108)
                     /gene="tdc"
                     /locus_tag="ACG8H7_000060"
     CDS             complement(66273..68108)
                     /gene="tdc"
                     /locus_tag="ACG8H7_000060"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP8982205.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19443 a        14582 c        11700 g        22629 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atccgcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata
     9181 gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca
     9241 ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga
     9301 cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt
     9361 cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta
     9421 cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac
     9481 tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa
     9541 aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata
     9601 taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta
     9661 accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc
     9721 ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat
     9781 acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac
     9841 atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt
     9901 tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg
     9961 ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga
    10021 ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta
    10081 atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg
    10141 tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca
    10201 attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata
    10261 cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc
    10321 tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac
    10381 aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta
    10441 ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat
    10501 aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa
    10561 gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag
    10621 cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc
    10681 atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg
    10741 cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact
    10801 gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca
    10861 aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca
    10921 atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg
    10981 tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg
    11041 attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat
    11101 tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct
    11161 gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga
    11221 cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg
    11281 tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt
    11341 taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg
    11401 tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc
    11461 ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca
    11521 aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt
    11581 gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg
    11641 aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat
    11701 aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac
    11761 ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca
    11821 tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt
    11881 aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta
    11941 tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca
    12001 cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct
    12061 ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt
    12121 aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa
    12181 aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga
    12241 tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc taattcattt
    12301 aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc
    12361 aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat
    12421 tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc
    12481 atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt
    12541 aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc
    12601 acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc
    12661 aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca
    12721 tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat
    12781 gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga
    12841 gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga
    12901 gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca
    12961 taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct
    13021 aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata
    13081 actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca
    13141 aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct
    13201 ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc
    13261 atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca
    13321 ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct
    13381 ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca
    13441 actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc
    13501 tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga
    13561 cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc
    13621 cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac
    13681 ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca
    13741 gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac
    13801 tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt
    13861 ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt
    13921 cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt
    13981 tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa
    14041 aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat
    14101 tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt
    14161 attaatatgt caaccagaat gactcttcat ttttttcact actttttata tttgcataaa
    14221 ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga
    14281 taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa
    14341 aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc
    14401 tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa
    14461 acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat
    14521 taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat
    14581 ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg
    14641 tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct
    14701 tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt
    14761 cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg
    14821 aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag
    14881 tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga
    14941 atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    15001 cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    15061 ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa
    15121 atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    15181 gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
    15241 aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat
    15301 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg
    15361 gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    15421 acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt
    15481 caaaaacaac ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg
    15541 agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga
    15601 aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg
    15661 acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc
    15721 atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg
    15781 atatttttag atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat
    15841 agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt
    15901 tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat
    15961 ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct
    16021 gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg
    16081 aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc
    16141 attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac acggggaata
    16201 aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt
    16261 gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt
    16321 cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac
    16381 gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg
    16441 ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata
    16501 catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga
    16561 caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg
    16621 taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt
    16681 aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag
    16741 cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat
    16801 cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc
    16861 cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga
    16921 tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca
    16981 acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg
    17041 tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga
    17101 cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca
    17161 ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca
    17221 agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg
    17281 gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat
    17341 tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga
    17401 tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt
    17461 gatattcttc actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa
    17521 tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat
    17581 ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa
    17641 tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg
    17701 actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg
    17761 cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct acatttatca
    17821 aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa
    17881 aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg
    17941 gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca
    18001 taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac
    18061 gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat
    18121 agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa
    18181 tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa
    18241 gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat
    18301 tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact
    18361 ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa aacatacccc
    18421 attcttctag gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa
    18481 actttagtat gttttataga tttacaccat taatgaatag acacctaccg cgataattgc
    18541 tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt
    18601 agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc
    18661 atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa
    18721 tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac
    18781 aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt
    18841 tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga
    18901 aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac
    18961 gacaagattc caactaattg cccctgtcat cgccatagct agagagactg caggattcaa
    19021 atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc
    19081 aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc
    19141 acacacacca tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg
    19201 agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat
    19261 caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc
    19321 agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag
    19381 atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc
    19441 ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc
    19501 taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc
    19561 ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt
    19621 agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc
    19681 tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt
    19741 acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa
    19801 agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt
    19861 caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc
    19921 gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga
    19981 accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa
    20041 tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc
    20101 ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta
    20161 agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa
    20221 gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt
    20281 tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca
    20341 aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag
    20401 gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat
    20461 tttacttact agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg
    20521 ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa
    20581 aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc
    20641 tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa
    20701 ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg
    20761 tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc
    20821 aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc
    20881 ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac
    20941 tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg
    21001 cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt
    21061 caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct
    21121 cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg
    21181 tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt
    21241 ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat
    21301 attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat
    21361 cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa
    21421 tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat
    21481 cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg
    21541 tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc
    21601 tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct
    21661 ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt
    21721 caccggtatt catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt
    21781 cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg
    21841 cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga
    21901 cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta
    21961 gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt
    22021 gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt
    22081 tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg
    22141 agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat
    22201 tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga
    22261 ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa
    22321 tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact
    22381 gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt
    22441 gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc
    22501 gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc
    22561 cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa
    22621 gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat
    22681 tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt
    22741 cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa
    22801 atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca ataccgccga
    22861 ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt
    22921 ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat
    22981 tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt
    23041 gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt
    23101 tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa
    23161 accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca
    23221 aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg
    23281 ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg
    23341 aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg
    23401 ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat
    23461 agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt
    23521 tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta
    23581 atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg
    23641 gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct
    23701 cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca
    23761 attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa
    23821 cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct
    23881 gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct
    23941 gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat
    24001 cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga
    24061 tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt
    24121 ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata
    24181 tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc
    24241 catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt
    24301 tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg
    24361 ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa
    24421 aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg
    24481 ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca
    24541 tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc atttgttgga
    24601 cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag
    24661 tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga
    24721 ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc
    24781 gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt
    24841 gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa
    24901 cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac
    24961 ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc
    25021 atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt
    25081 gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca
    25141 aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc
    25201 ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa
    25261 agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat
    25321 cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga
    25381 taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt
    25441 cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa
    25501 ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc catcataccg
    25561 tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg
    25621 aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc
    25681 tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag
    25741 ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa
    25801 ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc
    25861 tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa
    25921 gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac
    25981 tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac
    26041 accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc
    26101 aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc
    26161 tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct
    26221 gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg
    26281 ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac
    26341 taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt
    26401 ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat cctctttcta
    26461 taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa
    26521 agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat
    26581 aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca
    26641 tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg
    26701 atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca
    26761 gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca
    26821 cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct
    26881 tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag
    26941 aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat
    27001 tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct
    27061 ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat
    27121 gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga
    27181 cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt
    27241 gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct
    27301 ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc
    27361 cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta
    27421 gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt
    27481 tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta
    27541 attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc
    27601 gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa
    27661 attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact
    27721 tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac
    27781 ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct
    27841 aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg
    27901 ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat
    27961 caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa
    28021 tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt
    28081 ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt atgattttta
    28141 ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa
    28201 gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga
    28261 atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg
    28321 ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt
    28381 tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa
    28441 tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg
    28501 tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa
    28561 acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac
    28621 tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt
    28681 attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct
    28741 ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac
    28801 gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac
    28861 caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat
    28921 agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga
    28981 gtccgttttc agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa
    29041 ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt
    29101 taattcctcc agttattaag tttccttgga ccccttccca attttctgga tgagcactga
    29161 ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt
    29221 cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat
    29281 acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct
    29341 caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga
    29401 tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac
    29461 ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt
    29521 cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag
    29581 tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt
    29641 aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca
    29701 tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca
    29761 cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa
    29821 ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga
    29881 tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc
    29941 tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc
    30001 atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt
    30061 catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc
    30121 cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt
    30181 tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt
    30241 ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc
    30301 tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac
    30361 tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc
    30421 atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc
    30481 cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac
    30541 ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga
    30601 ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat
    30661 cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga
    30721 caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc
    30781 aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc
    30841 ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact
    30901 ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc
    30961 tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt
    31021 cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc
    31081 gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat
    31141 cagctgactt gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac
    31201 gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat
    31261 cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt
    31321 ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc
    31381 actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt
    31441 attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc
    31501 agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt
    31561 acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc
    31621 aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct
    31681 ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc
    31741 atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac
    31801 accttctagt tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg
    31861 taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac
    31921 aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt
    31981 agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt
    32041 accccttata atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa
    32101 atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa
    32161 aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt
    32221 gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg
    32281 aagcataact aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg
    32341 gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc
    32401 ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc
    32461 tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt
    32521 caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc
    32581 tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt
    32641 caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt
    32701 caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg
    32761 aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat
    32821 cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt
    32881 gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa
    32941 acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc
    33001 attaatcatc aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt
    33061 ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat
    33121 aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac
    33181 gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag
    33241 aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg
    33301 ttgaggcaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct
    33361 ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa
    33421 tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt
    33481 tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa
    33541 gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac
    33601 ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg
    33661 tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt
    33721 ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg
    33781 attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt
    33841 acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc
    33901 agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga
    33961 taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat
    34021 cattttgtct atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg
    34081 atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg
    34141 cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc
    34201 gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta
    34261 attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc
    34321 aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc
    34381 cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg
    34441 caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg
    34501 cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga
    34561 catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat
    34621 cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat
    34681 ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac
    34741 aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag
    34801 ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa
    34861 aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt
    34921 caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg
    34981 gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga
    35041 ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa
    35101 atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac
    35161 gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg
    35221 attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct
    35281 ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg
    35341 caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt
    35401 cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat
    35461 tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac
    35521 tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa agaaaacact
    35581 cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt
    35641 aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa
    35701 gcttcacgtg gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat
    35761 tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat
    35821 tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac
    35881 atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt
    35941 ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat
    36001 tgcataggcg tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct
    36061 tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct
    36121 tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa
    36181 ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac
    36241 acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa
    36301 ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca
    36361 gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg
    36421 tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg
    36481 atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt
    36541 gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta
    36601 tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg
    36661 atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa
    36721 tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc
    36781 caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca
    36841 cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac
    36901 ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc
    36961 tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct
    37021 tgataattac tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc
    37081 caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa
    37141 tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact
    37201 gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt
    37261 tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt
    37321 gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg
    37381 ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac
    37441 ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat
    37501 tttttcgttt ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa
    37561 cggtgttcac tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta
    37621 ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt
    37681 cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga
    37741 tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc
    37801 aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat
    37861 cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac
    37921 cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa
    37981 caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac
    38041 ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc
    38101 cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc
    38161 tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt
    38221 cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg
    38281 ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg
    38341 cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat
    38401 gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa
    38461 ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat
    38521 gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc
    38581 atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat
    38641 caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc
    38701 cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc
    38761 aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt
    38821 cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa
    38881 taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt
    38941 ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc
    39001 taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg
    39061 ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc
    39121 cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt
    39181 acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt
    39241 gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag
    39301 gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct
    39361 tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat
    39421 acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa
    39481 tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa
    39541 gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt
    39601 tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac
    39661 ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca
    39721 atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct
    39781 acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt
    39841 atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt
    39901 atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt
    39961 ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaaaacgg caatgaatgc
    40021 cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt
    40081 gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct
    40141 gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg
    40201 tttcttccgt cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc
    40261 cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta
    40321 aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt
    40381 cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta
    40441 caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa
    40501 tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa
    40561 tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct
    40621 cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat
    40681 ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta
    40741 ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc
    40801 tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt
    40861 acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt
    40921 ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg
    40981 tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc
    41041 agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac
    41101 taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc
    41161 ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa
    41221 tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc
    41281 gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt
    41341 ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc
    41401 ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa
    41461 aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt
    41521 atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt
    41581 tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag gattttcgca
    41641 aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt
    41701 gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc
    41761 atcccacctt tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat
    41821 tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt
    41881 tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc
    41941 ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg
    42001 gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca
    42061 agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct
    42121 cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt
    42181 tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt
    42241 ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt
    42301 ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt
    42361 cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca
    42421 aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga
    42481 gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa
    42541 gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag
    42601 aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc
    42661 gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt
    42721 cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa
    42781 cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct
    42841 gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt
    42901 aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat
    42961 tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta
    43021 catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct
    43081 ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac
    43141 catagtgata atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt
    43201 accgacgttg acataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc
    43261 cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg
    43321 acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat
    43381 aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg
    43441 catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta
    43501 gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact
    43561 ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc
    43621 ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac
    43681 ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc
    43741 tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct
    43801 ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc
    43861 agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca
    43921 atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta
    43981 tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt
    44041 tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg
    44101 atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg
    44161 attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc
    44221 atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta
    44281 gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc
    44341 atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct
    44401 tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt
    44461 ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc
    44521 catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt
    44581 atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt
    44641 ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg
    44701 ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg
    44761 tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc
    44821 cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac
    44881 tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc
    44941 tactgtatct acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac
    45001 tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc
    45061 tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt
    45121 tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat
    45181 cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa
    45241 aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa
    45301 tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat acacacccac
    45361 tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc
    45421 agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac
    45481 catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc
    45541 gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa
    45601 gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat
    45661 gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac
    45721 caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc
    45781 catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa
    45841 aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa
    45901 atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa
    45961 tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc
    46021 actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga
    46081 tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt
    46141 caaaatattt tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt
    46201 atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt
    46261 gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac
    46321 tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa
    46381 atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac
    46441 atctgtgctg caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg
    46501 ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt
    46561 ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc
    46621 aattcaatta gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa
    46681 aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta
    46741 gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg
    46801 tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca
    46861 tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc
    46921 tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac
    46981 ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac
    47041 ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga
    47101 gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt
    47161 ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa
    47221 agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta
    47281 tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt
    47341 tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact
    47401 gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg
    47461 acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga
    47521 acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat
    47581 ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag
    47641 agattttcga ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca
    47701 aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga attggagata
    47761 tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca
    47821 ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt
    47881 atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc
    47941 cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac
    48001 ttattggaat agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag
    48061 attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga
    48121 ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc
    48181 agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac
    48241 cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt
    48301 agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc
    48361 cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc
    48421 ttttccagct tcaacaaaaa aactttgttg cgaattgtac ggatctccgg aagcttttta
    48481 ctgctcaacc agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg
    48541 aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt
    48601 atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc
    48661 gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc
    48721 aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta
    48781 ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa
    48841 gtaataagaa caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta
    48901 tcgcagatgc aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc
    48961 ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta
    49021 ttgccgatag gctatctctg gcacactgtt tattcagaaa aaggagctgc gacaaggctt
    49081 ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat
    49141 cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact
    49201 gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac
    49261 agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta
    49321 cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca
    49381 gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag
    49441 tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg
    49501 ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct
    49561 tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta
    49621 atgcaagaaa tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat
    49681 aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa
    49741 atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag
    49801 cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat
    49861 aattgatttt tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat
    49921 agccttgcta aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg
    49981 tcttatttag tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc
    50041 tatcttcagc atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg
    50101 gttttcctga caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact
    50161 agcagcatga gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac
    50221 gcatgtgtca tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata
    50281 tgaatcatta tctagctcct tagctgcttg gattgcaagc ctgatatgcg gataccctaa
    50341 tatcgcttct tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt
    50401 ggttttttta tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag
    50461 tattttcgat aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa
    50521 tagccccatt gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat
    50581 tgccttttcc gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc
    50641 atgatgccat gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt
    50701 aagaaataaa gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc
    50761 aggttttgga cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg
    50821 ctgagtcact gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta
    50881 ttacttgctg aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc
    50941 aaatgcacaa cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg
    51001 cagccaacta tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata
    51061 cgtacgataa gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac
    51121 aatcattttg gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg
    51181 gatcgtattc aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga
    51241 acggacagaa aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc
    51301 caaatattga tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact
    51361 agcaacaaac cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt
    51421 ttgatagaac agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac
    51481 atatttttta ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg
    51541 gatctgcagc atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt
    51601 cagtaaaaag cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca
    51661 aagataagcc gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa
    51721 aaattcccaa taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat
    51781 aaacattggt gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt
    51841 tgcgctgtgg ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac
    51901 agctgatact ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc
    51961 tttctctctc tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt
    52021 ttcaatagaa tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc
    52081 cgagtcacta cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga
    52141 attggcaatt caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt
    52201 actgccggaa tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat
    52261 cctgataata tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc
    52321 tcttgtctat acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca
    52381 tgttttgaga tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt
    52441 agtatttctg cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga
    52501 acgacccatt caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca
    52561 tgtgcttgaa tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt
    52621 gtttcgacac gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg
    52681 tcatttgtat tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca
    52741 ttttgttctg aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt
    52801 tttgtataat tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt
    52861 tcagcaaccg aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta
    52921 atttctttat tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca
    52981 cctgaaatag catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt
    53041 gcataagcag cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag
    53101 ccgtatgctt ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct
    53161 ttatcttctt cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca
    53221 acttcaccgt ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg
    53281 atggcttctt ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt
    53341 ggatttttca tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt
    53401 tcatttcctt ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct
    53461 tcattgtaat cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa
    53521 taaaatgctg agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca
    53581 gcagctttga ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc
    53641 cgttgacgag atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga
    53701 acgcctgctt cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt
    53761 gcgattaaat aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt
    53821 cctggaatca aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt
    53881 tctgaaatag ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt
    53941 acaatgactg gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca
    54001 gcaacgattt ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata
    54061 gcgattacta ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca
    54121 ctttcaggga cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca
    54181 atcaattcac gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca
    54241 aaactatttt tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc
    54301 tggtgtagca ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa
    54361 tctcgttaat acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac
    54421 agtagattct gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac
    54481 tcctacatgg attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc
    54541 catggatctc tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt
    54601 cttccatctg ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg
    54661 aatttcattg gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt
    54721 gcagctaata atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc
    54781 acgatgccgg ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt
    54841 gtaacaccgc caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac
    54901 aaataagcaa ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact
    54961 ggtgcattgg ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag
    55021 actggttttt tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc
    55081 cctaatggat catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg
    55141 ccaggattat gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg
    55201 gtagaagagt ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca
    55261 tattgtcctg cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca
    55321 aaaaagacat ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta
    55381 tggatgcttt caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat
    55441 acccagttct tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac
    55501 aattcgatag ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct
    55561 gcgtagattc ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga
    55621 ggatctcgtg tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct
    55681 acccagtcac cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg
    55741 atcacgatac catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg
    55801 aaacctgtgt acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt
    55861 ggaatcaaat cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat
    55921 ttcattgtgc cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag
    55981 aaatcgatct tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata
    56041 ctagacatga tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct
    56101 gaaagaaaac ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca
    56161 atcagttttt ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag
    56221 ttgctgattt tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt
    56281 ggtttttcca taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca
    56341 tgttggtcta gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc
    56401 tttgtcaaag tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc
    56461 aagtccatca tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga
    56521 atctcttctg cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca
    56581 tcagatagat ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag
    56641 agacagtgag atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg
    56701 gcttctgcca acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga
    56761 caattttcat ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc
    56821 tcagcttcgc ttctcgtcac acctactact acgcccattg cataaccgct tggataagct
    56881 tctttcatta agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat
    56941 cgacaagcaa cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga
    57001 ataccgtttt tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg
    57061 ataaaaaccg tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt
    57121 tctaaacgtt ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct
    57181 tgtccaggaa ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc
    57241 actgctatct tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa
    57301 caaaaaggta tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa
    57361 agtattcgaa acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc
    57421 cttgagccgt gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag
    57481 cggcagaaac attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac
    57541 attagacaca ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac
    57601 ttccgtcata ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt
    57661 tatggaaaag catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat
    57721 taccgatcct gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat
    57781 catgaaagaa cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca
    57841 tctgcaatta gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt
    57901 ccgtcataca ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac
    57961 gacagatgat cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt
    58021 cccaaaagtt tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga
    58081 tttgcccttg acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat
    58141 caaaatgatt gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg
    58201 ggacaaaatt cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt
    58261 agaaaataag ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat
    58321 ttcttgaagc taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg
    58381 ttgatttaaa acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta
    58441 gatcaataga gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc
    58501 ctcgcaaaat tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat
    58561 ggtcacttgc atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt
    58621 ttttgctttt ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg
    58681 gagacaaatt gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct
    58741 tccatctctt tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga
    58801 atacaagtag ttgctaatca aatacttcga tatttttctt ttttatcaac tcttaaatgt
    58861 tcaatccgcc tgtgttggtc ttgctaccat ctactattaa aatgtttatt attgtttgcc
    58921 cacctatttt ttgattttag tggtatcatt tttcaagttc atgatgaaat tcttatcatc
    58981 agtaaatgct ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt gaccaccgtt
    59041 tattttctcc gtattcttag aatagaaggg agtctgattg atgtattcac taatgaaaaa
    59101 aatcattacc gaaaaagata ttcgacgtca tgtttcttta gtagaacaat tgctgaatca
    59161 tacaaagata accgtaaatg aattagctga aatcataggg acaaccgaac gaacgatttt
    59221 ttctgacctt caatctattc gctctcagct gcccgaaggc tgggacatca tcagcgatca
    59281 agcaggaatc agtttgcaaa accagcaaaa tttattaaca aatgaccttt gggagatttt
    59341 tttcaaacaa tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa aaaaagttgc
    59401 tgtccctgat tttctagccg accatgggct ttcttatggg acattgaaaa gacatgtgac
    59461 taaaatcaat cagcgtttag caagttacga cttgcagatc gatttgacga aatatacagc
    59521 ttgtattctt ggaaaagagc gtgcgattcg tactttttat catcgattgc tgattccttt
    59581 cactcataac aactattttt tcgaggatta ttcgatccat gaatcccatt attttcaatt
    59641 tttaagaaac ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt tcggaacatg
    59701 ttggttcttt atcaatacga tccgtataaa agcgaattgc cgattagatt cttctatcca
    59761 taccaacagc acactctcct ccctgtatga ctctgcattg aaaaaattgt atttaacgga
    59821 aggtatttat cttaaagaca ctgagctttc ttttgcttct ttctgctttc tggaaagttg
    59881 gaattacaac aataactatg gacaagaaac cgctcgctgc ctgcatcatt ctccattttt
    59941 agaggtcctc gaaacgtttg tagaggaact agcttcagag ctgtctcttg atcaactaaa
    60001 aaaaacatct ctgactgata acttggctct cctcatatta aaatatcatg aatcgccgat
    60061 attgattgaa gcattggacc gtcaatacca tcgtttttta gaaacatatg atcagcaata
    60121 tgcacacttg tatcaacaga aagagaattt actgaacgat ctaaaaaaag aaatagaaat
    60181 caatgaacca aattactttc tccaactctt gtctctactg atccaacaaa cgattttttc
    60241 tatcaaaccg aatttgttca atgtctattt tattttccaa ggagaacctt catggaaggc
    60301 gttcttgcag caggaattaa aagattactt aggaaaacgt gtctgcttct tacctatcga
    60361 actaaccgat ctttccacta tttcttttga aaaaacagat atcttgattt ccaatttccc
    60421 tctagattct ctagatatcc ctattgtata catttcttct attccaacga aaaatgagct
    60481 caatcgtttg actgaactca ctttcaaaac ttttttatag taacggctag cgactgtatt
    60541 ctctagctgt tttttctttt ttgcgaaaca gtttgttcat gatccctttt tctatttcta
    60601 caactaagaa tagaagaatc acatttgctc caatcaataa ctgttgtgtc atcgttaaac
    60661 ttactgtacc gatcagctgc tggccgactg gtagaaaaat aaccatccct tgcaataaag
    60721 caaggatacc taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg ttcaatgacg
    60781 gcttggacaa ctctctgcaa ttgatcatgt aaacagcttg agctaagacg atattttgca
    60841 gaagaatggt ttgctgtaag ctttgccctt caaactgcat agccaataaa taggcaggaa
    60901 ccatgatcag tagggagaca tagatgatcc gaaacaaatg ataaccagat aggattcctt
    60961 gctttggatc tcttggtggg cgcttcatga tttctggatc tggcgactcg aaccctagtg
    61021 cataagataa agtgattgtc gttaccatat tgacccataa aatctgtact ggggacaaag
    61081 gtagcgggcg attcagcata agtgcccaaa tcaagatcaa tccttgagct aatgcagttg
    61141 gcaagaagaa attgatggtt ttttgcaaat tatccaaaat ccgccggccc tcttttacgg
    61201 cttttgcaat cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc tgtttgctta
    61261 cttcactacc tttgattccc atagcaattc ctacatcgga tcttttcaaa gcaggtgcat
    61321 catttacgcc gtctccggtc attccgacga tttctccatt tttctgcaaa gctttaacaa
    61381 ttcgcagttt atgttctggt gtcgttcgag caaagacatc gactattggc acttgctgaa
    61441 tcaactcttc atcagacata agatccaaat cgatcccttc taaaactttg gcagtatgtt
    61501 ttaagccaat ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt
    61561 tcactgagat ccctgcttgt tggcattcct ttacagcttt gactgcactt tcttttggcg
    61621 gatcgatcaa tccggcgatc cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt
    61681 cctgtttcga atctactttt ttataagcaa atcctaaaac acgctgacct tttctcgcaa
    61741 attctgcagc tgtctgactc catgcttctt gttctgcaga agaaagagtt gatttagcaa
    61801 ataatacttc tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg
    61861 ttgccatata tttgtattca gaactgaacg gaatctttgc ttctattttt ttcaaaggta
    61921 gattggctgt ttctgtgtgt cgaatcagcg caacttctgt cggattgcca cttaaatttt
    61981 ctgttttctg ttcttctttt gtttttaatt cctgacagtt tttcataatg gacaacacgc
    62041 aggcttcttc ttttgcagct acatccacaa ctgtcatttc attttttgtc aatgttcctg
    62101 tcttatctga acagatcact gtcatagaac ctaatgtttc taccgaagga agtcctttga
    62161 tgatcgcctt ttcttttgcc atttcatgta ctccaacgga tagaatcatg gtcaaaacag
    62221 ctggcaatcc ttcgggtacc attgacacca ccaaagcaat cgtagccgaa agaaggatat
    62281 gccaatccat accatatcga aatgtcgtaa aaataactaa gaataggctc aaacaaagaa
    62341 tgccttgaaa aatttgtttg tttaatcggt tgattttttt aatcagaggc gtcgtttgtt
    62401 gttcgaccga ttgcaatgct tgattgattt taccgatttc tgtcgtgttt cctgtttcta
    62461 caacgacacc gactgcagaa ccagattgta caagcgttcc tgaaaaagcc atatttttct
    62521 ggtcgccgat cgattcttct tctgtcaaaa cggcatgact tttttcagat gcttctgatt
    62581 ctcccgtcaa aatcgattct tccactaata aatcatgtgc ttcgattaat cggatatctg
    62641 ctggaacgac atctccggca cgtaagacaa tgacatctcc ttttaccagt tctttcgcag
    62701 ggattttttc ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc agctgcttca
    62761 aaccattcaa tgattcttcc gcttttcttt cttgccagta actaacgagt ccattgacga
    62821 tcactacgaa taaaataata cttccttcaa tatattcact agaagcaaat ttcaaaaaag
    62881 aggcaatgat caacacgatc atcagcagat cagtaaaatg ctttgctatt tttttccatc
    62941 gttttgtctg ctgccttgct tttatttcat tatatccttg atcagtcagg cgtttctgtc
    63001 gttcttcttg cgacaagcct tctgttgatg tttgatagtt tttcaataca tcttctaccg
    63061 tcaatctgta ccagttcatc ctatcccctc ctgtttaatt gatactgtta ttatacgcag
    63121 ggaagttttt tcaggcgtat cgattatttc aggggggtct gaaataattc gttttgcaaa
    63181 atgtatctgg gcctaactga tatttctatt gcttattctt ttatatacaa aatttaagaa
    63241 tacaaaccag ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct
    63301 gaaagcaatt gaagtaaaaa acaccaacaa agcaagatct ttatatcttt gtgtagtgtt
    63361 ccgtttcttt tctttaataa attgaacatg tgcacacaag atcgaagacc acacaaatag
    63421 aaaacaactc gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa
    63481 aaaatgtaga caaagaccgg caaaaaataa cattcctgta attaaaatac cgtgtaaagg
    63541 tacgcctttc ttagaaagcg cacaaaacca atggggcaaa cttttttctt ttgctgtttg
    63601 atataaaatc ctgctagtac tgtagactgc actatttgct gaggacaaag aagcaaccaa
    63661 aatcattaga ttgacaattg ctgctccgcc ttggcaatgg atcacttgca gcatagtcac
    63721 taaaggactt tcttttaaat ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat
    63781 cgttcctata tagaaaaaga gaatacgcca aggcagacag ttgattgctt ttttcaaatt
    63841 cttttgcgga tcacttgtct cgccaacggt taagccgatc atttcaattc cagcaaaact
    63901 gaataaaacc ataggaaatg agactaccat tccagcaaag ccaaacggta gtaattcatc
    63961 gatctgtcca aaattttctt tgattccgat agactgggta tgaaaatctg agacactcaa
    64021 tcctataccc aataagataa aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga
    64081 ttccaattca ccaaaccact tgactgcaac aaggttgact gccattaaga tcattagtgc
    64141 gctacctccg cttatcaaag ctggtacatg tacaaaccag aaatggatat acaatcctat
    64201 tgctgttaat tccgcaagtc cagctccaag ccagcaaagc caataagtcc aattgacaaa
    64261 aaatgcccac tttttgccta aataatagtc aacgaaatca ctgatagagt taaatgtact
    64321 atttgctaaa aataaatcac ccatggcccg catcaaacca taaatagcca aaccgcatat
    64381 caaataaacg agaataacag ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa
    64441 taatcctgtt ccaattgcgc cacctaatgc gattaactgg atatttcgat tagttagagt
    64501 ccgtttcatt gccggttttt ccatcaagtt cacctttttc tttttcaagc agcatggcga
    64561 cttcctgttc ccaagaaggt ttctttattt tttcaataca gagtggtact acaaacaaaa
    64621 tgacactgat ggctgtgact gcaattagat tgatccatgt tccagctttc atcgtcagtc
    64681 cgacagaaaa tccgattgcc gcaaataaaa caataacgac agtccaaact ccaagattgc
    64741 tctttttctc tttttttcca attctaaatg gtcgattcaa ctttgcatgt gtataacgca
    64801 atctcaaaac tgcgatcccc atgatgacat agacgataca gtagataaca gtagttgaat
    64861 ttacgagcat caaaaatgct tgattgacgc caggaatcag caaataaaaa agtgcaaaga
    64921 ttgagataat gacagactga gtcaaaataa cggatttgga tacgtcaaat tgattcgtct
    64981 tccaaaaatt gaattttggt ggatacgcac cttttctagc actttctgtc actgtctttg
    65041 ctggtcctga ggcccaagca gataattgga ctgctacacc aatgaagaca agtaaactaa
    65101 acagattcac aataagatgt ggcaaaccaa gaatttggca ataaatcgaa atagattgcg
    65161 cgatattatt caattccatt tgtccttttg gtacgacatt tgctacaact aaagcattca
    65221 aaatattgac gaaaaatata aaaattaacg cggcaaatac tccgcgaata tacgttttaa
    65281 ctggattttc caatcgagtg atatacacag aggacatttc gataccagta aaaataaaca
    65341 tgatcggcgc aaaataaaca aatgaggatg cagtcgtagt atctggtatt aatttgtccc
    65401 atgaaaaact gcctaatgtt ccggttggta cgatcccaac tttgatccac gcagcaaacc
    65461 ctaataagag catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg acttttccgc
    65521 cgatttttgc catatcaaat ttcaaattca aaagagtgat gatccaataa acgactaaaa
    65581 tgacaactaa tgtaaatttg ctgttcaaac caagcggcac attatcgata atattaccaa
    65641 acaacggagc gagtgctgaa gcgaccatca ccatcccagg aaacatttgg acccataaca
    65701 gccatgagac aacgaacccc catttttttc ctagagcatt cgatacccaa agttctggac
    65761 cacctttttg gggaaacatt ccagcaaatt caccagagat caatgagatt ggaaaagcgt
    65821 acaataatac tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg
    65881 gaatatttcg tacacttgct acaagggcgc aggtcattcc gataaaggtc atggtcgtga
    65941 tttgctttga tgaaccagta tctttcatct ttattccttc tttcaatagc gacatatgtt
    66001 tttgttttct tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca
    66061 aaacccagta tcaccgaaac atccattgaa cgatcttagt gaactgggtt tgatacatga
    66121 aacgaacgat ccaacttata gaacgtttta taaaaaagaa tatcttggcg aggaatatat
    66181 gaaaaacagg gggaaataca attgttttga tgaagcaaat aattgtctgt tctccatatg
    66241 tctcttgtta ttctttcaca caaaaatcag ttctattgga tgatttcgtt tagtttcttt
    66301 tccattgctt tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc attcaaataa
    66361 ggagtcatga tagccgcacg tagcagagtg attttctgtt cttttttcca ttcttctttc
    66421 gttagaccca ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat
    66481 gtagtatgag aagtaataaa ccgttcacca taaacatcgc catccatata agaagaccga
    66541 tcgaacattt tttcatttaa ttcattgata gcgttcaaat ctgtacagcc ttgttctttg
    66601 aatacccaat tgaccatatt aaaatcagga tgattcaatg gatagacttc gattgttttt
    66661 cctttaacag taaaagtcag atgatcgaga aattctctga atctttgagc tgcctcgatt
    66721 gaagccccaa tcaattgacc atatccggtc acattcaatg gcaatacacg atgtgctgtc
    66781 cagactgcgg cggcggttgc tccagctttt gatccttcca aaatataagc tcctaacata
    66841 tctggagcct ttactgattt ttcaaacaca tatggcgcga aataagaaat gatatttctc
    66901 atatttttat gtttgattac gattcctcca gcagcataag ggacatagcc catcttatga
    66961 ggatctatcg tgacagaatc cgcttctgaa atagctctga acccttcata tacctctttg
    67021 tcaatcgtta cgcagtggtg gaaaacatga tgttcttcaa agaattcagc taaggaagca
    67081 tacggcacaa attctcctgc ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg
    67141 gcatcaacat gtaaatagaa atagatccct tcatctttta acctttctct taattggacg
    67201 attttatcta cactgtctac ttgaccttct tctgtcgttc ctacgacagc aaccacacct
    67261 agaattggga ttttttgtcc agctaattca cgaattgttt tttccaaagc attaatatcc
    67321 attcgataat cctcttgaac tgggatagca accatctgat ctaagccgac tccgcaaata
    67381 tctaacgcct tcatccaaga atagtgtttt gtttgaggga ccagccattt ccctaatctt
    67441 tggatatttt ttccgctacg ggaagaagct gctttgacct catccatttc ttcgtccgta
    67501 aagctttcgg tcatttccaa aatttcttca acagataaat tcaaaagttc ccactcagac
    67561 atttttttca ctttttcagg cagcacttcc ttgacagcta aaggaatcga tttgatgcaa
    67621 cgggcgtacc ataatccttc aagattagca atagaaccat ctgctgcaat gtgtccccat
    67681 ccgtctgtca tattgaataa tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa
    67741 gttgccatag aagattccaa tgcaacatta tttgggttcc acagcatcgc atagttgtaa
    67801 gctaataaag caggcatcaa tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa
    67861 tgccatggga tcgatcctgc gcgtaatcgc tgactgactt catccaagac ctctatcata
    67921 tggtcgcggg tggctagata atctggagtg agcttatctc cttcgctaat tgcaggcata
    67981 tctgaaggaa tataattttc tctccatcca agatgttcat ctaccatctt gttcaacaac
    68041 attttataaa ctggtccatt ttcagctttg tcgcctataa agacggcctt gatatccata
    68101 tctttcattt ttcttccctc catattttgt tggtcaatgt cttgcagata catgtgttta
    68161 tctgtaacac ctcttcacta tagcgccaaa aatgagaaat ttttttttat aacatcaaat
    68221 ccacgtagat ttttacaaac aaaaataaat aaagtcggat ttttctgata attcaacgca
    68281 aaaaagcgaa caatttttca ataaatcagt cttctaaaat agataaccta aaaaaagcga
    68341 acaaacaaat aaaa
//
LOCUS       DAZKAS010000002        56054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000002 DAZKAS010000000
VERSION     DAZKAS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.002"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..196
                     /locus_tag="ACG8H7_000061"
     CDS             <1..196
                     /locus_tag="ACG8H7_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP8982206.1"
                     /translation="TLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGIT
                     RQGATNLKHRLYKKIQKMLV"
     gene            290..481
                     /locus_tag="ACG8H7_000062"
     CDS             290..481
                     /locus_tag="ACG8H7_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982207.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            995..1327
                     /locus_tag="ACG8H7_000063"
     CDS             995..1327
                     /locus_tag="ACG8H7_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8982208.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1237..1431
                     /locus_tag="ACG8H7_000064"
     CDS             1237..1431
                     /locus_tag="ACG8H7_000064"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8982209.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            1470..1616
                     /locus_tag="ACG8H7_000065"
     CDS             1470..1616
                     /locus_tag="ACG8H7_000065"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982210.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            2054..3394
                     /locus_tag="ACG8H7_000066"
                     /pseudo
     CDS             2054..3394
                     /locus_tag="ACG8H7_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3635..5251)
                     /locus_tag="ACG8H7_000067"
     CDS             complement(3635..5251)
                     /locus_tag="ACG8H7_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP8982211.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            5369..5587
                     /locus_tag="ACG8H7_000068"
     CDS             5369..5587
                     /locus_tag="ACG8H7_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982212.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5782..6243
                     /locus_tag="ACG8H7_000069"
     CDS             5782..6243
                     /locus_tag="ACG8H7_000069"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8982213.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6436..6675)
                     /locus_tag="ACG8H7_000070"
     CDS             complement(6436..6675)
                     /locus_tag="ACG8H7_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982214.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6699..8222)
                     /locus_tag="ACG8H7_000071"
     CDS             complement(6699..8222)
                     /locus_tag="ACG8H7_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP8982215.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8240..8362)
                     /locus_tag="ACG8H7_000072"
     CDS             complement(8240..8362)
                     /locus_tag="ACG8H7_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP8982216.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(8392..9375)
                     /locus_tag="ACG8H7_000073"
     CDS             complement(8392..9375)
                     /locus_tag="ACG8H7_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP8982217.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(9399..9548)
                     /gene="rpmG"
                     /locus_tag="ACG8H7_000074"
     CDS             complement(9399..9548)
                     /gene="rpmG"
                     /locus_tag="ACG8H7_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP8982218.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(9569..9946)
                     /locus_tag="ACG8H7_000075"
     CDS             complement(9569..9946)
                     /locus_tag="ACG8H7_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982219.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9978..10157)
                     /gene="rpmF"
                     /locus_tag="ACG8H7_000076"
     CDS             complement(9978..10157)
                     /gene="rpmF"
                     /locus_tag="ACG8H7_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP8982220.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10172..10441)
                     /gene="rpsN"
                     /locus_tag="ACG8H7_000077"
     CDS             complement(10172..10441)
                     /gene="rpsN"
                     /locus_tag="ACG8H7_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP8982221.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10964..12730)
                     /locus_tag="ACG8H7_000078"
     CDS             complement(10964..12730)
                     /locus_tag="ACG8H7_000078"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982222.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12690..14443)
                     /locus_tag="ACG8H7_000079"
                     /pseudo
     CDS             complement(12690..14443)
                     /locus_tag="ACG8H7_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(14553..15122)
                     /locus_tag="ACG8H7_000080"
     CDS             complement(14553..15122)
                     /locus_tag="ACG8H7_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFP8982223.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(15140..16555)
                     /locus_tag="ACG8H7_000081"
     CDS             complement(15140..16555)
                     /locus_tag="ACG8H7_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982224.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(16566..17234)
                     /locus_tag="ACG8H7_000082"
     CDS             complement(16566..17234)
                     /locus_tag="ACG8H7_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP8982225.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(17364..17948)
                     /locus_tag="ACG8H7_000083"
     CDS             complement(17364..17948)
                     /locus_tag="ACG8H7_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8982226.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(18279..18512)
                     /locus_tag="ACG8H7_000084"
     CDS             complement(18279..18512)
                     /locus_tag="ACG8H7_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP8982227.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(18527..19477)
                     /locus_tag="ACG8H7_000085"
     CDS             complement(18527..19477)
                     /locus_tag="ACG8H7_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFP8982228.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(19474..20316)
                     /locus_tag="ACG8H7_000086"
     CDS             complement(19474..20316)
                     /locus_tag="ACG8H7_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFP8982229.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(20318..21037)
                     /locus_tag="ACG8H7_000087"
     CDS             complement(20318..21037)
                     /locus_tag="ACG8H7_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP8982230.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21585..21887
                     /locus_tag="ACG8H7_000088"
     CDS             21585..21887
                     /locus_tag="ACG8H7_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982231.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            21959..23185
                     /locus_tag="ACG8H7_000089"
     CDS             21959..23185
                     /locus_tag="ACG8H7_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8982232.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(23273..23665)
                     /gene="rpsI"
                     /locus_tag="ACG8H7_000090"
     CDS             complement(23273..23665)
                     /gene="rpsI"
                     /locus_tag="ACG8H7_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP8982233.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(23681..24109)
                     /gene="rplM"
                     /locus_tag="ACG8H7_000091"
     CDS             complement(23681..24109)
                     /gene="rplM"
                     /locus_tag="ACG8H7_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP8982234.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(24643..25110)
                     /locus_tag="ACG8H7_000092"
     CDS             complement(24643..25110)
                     /locus_tag="ACG8H7_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFP8982235.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(25230..26768)
                     /locus_tag="ACG8H7_000093"
     CDS             complement(25230..26768)
                     /locus_tag="ACG8H7_000093"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP8982236.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            26848..27534
                     /locus_tag="ACG8H7_000094"
     CDS             26848..27534
                     /locus_tag="ACG8H7_000094"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP8982237.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(27735..28262)
                     /gene="msrA"
                     /locus_tag="ACG8H7_000095"
     CDS             complement(27735..28262)
                     /gene="msrA"
                     /locus_tag="ACG8H7_000095"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP8982238.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(28346..29023)
                     /gene="pgmB"
                     /locus_tag="ACG8H7_000096"
     CDS             complement(28346..29023)
                     /gene="pgmB"
                     /locus_tag="ACG8H7_000096"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP8982239.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(29020..30150)
                     /locus_tag="ACG8H7_000097"
     CDS             complement(29020..30150)
                     /locus_tag="ACG8H7_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP8982240.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(30150..32333)
                     /locus_tag="ACG8H7_000098"
     CDS             complement(30150..32333)
                     /locus_tag="ACG8H7_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFP8982241.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(32330..33355)
                     /locus_tag="ACG8H7_000099"
     CDS             complement(32330..33355)
                     /locus_tag="ACG8H7_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8982242.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(33400..34227)
                     /locus_tag="ACG8H7_000100"
     CDS             complement(33400..34227)
                     /locus_tag="ACG8H7_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP8982243.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(34224..35291)
                     /locus_tag="ACG8H7_000101"
     CDS             complement(34224..35291)
                     /locus_tag="ACG8H7_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP8982244.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(35317..36153)
                     /locus_tag="ACG8H7_000102"
     CDS             complement(35317..36153)
                     /locus_tag="ACG8H7_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982245.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(36150..37019)
                     /locus_tag="ACG8H7_000103"
     CDS             complement(36150..37019)
                     /locus_tag="ACG8H7_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982246.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(37089..38372)
                     /locus_tag="ACG8H7_000104"
     CDS             complement(37089..38372)
                     /locus_tag="ACG8H7_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8982247.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(38386..40071)
                     /locus_tag="ACG8H7_000105"
     CDS             complement(38386..40071)
                     /locus_tag="ACG8H7_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP8982248.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            40346..41371
                     /locus_tag="ACG8H7_000106"
     CDS             40346..41371
                     /locus_tag="ACG8H7_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8982249.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(41966..45619)
                     /gene="rpoC"
                     /locus_tag="ACG8H7_000107"
     CDS             complement(41966..45619)
                     /gene="rpoC"
                     /locus_tag="ACG8H7_000107"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP8982250.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(45698..49315)
                     /gene="rpoB"
                     /locus_tag="ACG8H7_000108"
     CDS             complement(45698..49315)
                     /gene="rpoB"
                     /locus_tag="ACG8H7_000108"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP8982251.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            50043..51011
                     /locus_tag="ACG8H7_000109"
     CDS             50043..51011
                     /locus_tag="ACG8H7_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8982252.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            51029..51934
                     /locus_tag="ACG8H7_000110"
     CDS             51029..51934
                     /locus_tag="ACG8H7_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982253.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            51931..52737
                     /locus_tag="ACG8H7_000111"
     CDS             51931..52737
                     /locus_tag="ACG8H7_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982254.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            52894..53862
                     /locus_tag="ACG8H7_000112"
     CDS             52894..53862
                     /locus_tag="ACG8H7_000112"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFP8982255.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(54179..54703)
                     /locus_tag="ACG8H7_000113"
     CDS             complement(54179..54703)
                     /locus_tag="ACG8H7_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFP8982256.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            54990..>56054
                     /gene="scm"
                     /locus_tag="ACG8H7_000114"
     CDS             54990..>56054
                     /gene="scm"
                     /locus_tag="ACG8H7_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP8982257.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        16911 a        11358 c         9566 g        18219 t
ORIGIN      
        1 gactctcctt gttgaagata taaaattagc aaaaatcctt cctttgttga aagaacctga
       61 acaaattttt cttatcgaaa aatttatctt tgataaaact gataaagaaa ttggaaaggt
      121 tctggggatt acaagacagg gtgcgacaaa tttaaaacat cgactttaca aaaaaattca
      181 aaaaatgctg gtttaatatc tgcataataa ctaattttgt atgcttcttt gctcgttctc
      241 ctccttccca aagaaataga ttataagtcc atgggaagaa ggtagattta tgaattcttt
      301 aaaagaagat ttcattttag caaaggctgg aaatgaagaa gctgttgagg caattctcaa
      361 aagatttagc tctttgattc ataagcaatc ctggagaact ggaaaatatg atcaagattg
      421 ttatcaagaa tgtatgcttg caatatatct agctatctcg aaattcgaaa ttaaagaata
      481 actcaactaa gggagtctgt gacataagta aaaagatagt ttcaaatttc gaacaataaa
      541 tttgaaacta tcttctttat aattataaag tatcgatttt acagcgttct ataatgattc
      601 atagaatcta aaacgaatga ttttaattat caacgttttt taattcgttt ttagcttaaa
      661 aataactttt gtcacagtct cactcctaaa attactttat ttacgtatga agtgggcagg
      721 aggtcgaatc cagaactggg ctatggtact tcaacgagga cttctcctct agaattgaaa
      781 agcatgcgat ttaccttccg taacgacggt agatttattc aactatgtgt ccaatcaaaa
      841 tttgtctgat ttcaaaaata gtcaagagaa ttttggttgt taaaaactct tatctatcaa
      901 cttccacagt ttagttgaca aaccctgata ctaagttggg ctatgataca cttttcttga
      961 ctatcagtta cgttaccgga aggaatgaac tctattgaga aaaacatatg atcgtaaatt
     1021 taaactaaag atttcacagg atatccttga gaaaaaagta actaccaaga aaattgctga
     1081 agaatacaat atctctcgtc caacaatttc ccgatgggtt tcagaatatc gtcgatacgg
     1141 gaaaaatgct tttgctggac aaggaaaaag attacctgat aaagcagatt tctacattct
     1201 tgaacaggaa aataaacgac taaaagagga agacgagtgt tctacgaaca caaagatcga
     1261 tacggatctc cagggatcac tcaaaaactt tacgatgaag gaatcgaaat caataaacgt
     1321 gttgtagctg ttcttatgcg tgaaatgaat ttatgcacaa gaggatttca tcgacgcaaa
     1381 tcttctcatg gtagagaaaa aacaatcaaa gaaatggtaa aaaaatgttg aatcgataat
     1441 ttgaacagaa ccaaattgat gctgtttggg tgatggatat tacttacatt ccatgtaatg
     1501 acggtcgcct ctatttgtcc acctatattg acttagcaac tcgtattcta cgttactata
     1561 tggtagattc acacatgaaa aaagagattg tcgtccgtcc attacaaatt tattaaggag
     1621 aattgcctac ggtaattcat tccgatagag gtagccagca tcggtctttt atttatcaag
     1681 agctgttgaa cgaccaccac atcacacatt cagtgagcca aactgggact cctgttgaca
     1741 atgcagtaat tgaagcgttt catcgatcaa tcaaatgaga attaatcgac tccaacaaat
     1801 ataaaagtaa aatagagatg aaggtgttaa tccaagatta tttagaagac tactacccaa
     1861 acaaaagaat ccatacaaaa tttatgatga ctccttgaca atttgaagcg aagaaaataa
     1921 gggatctata atatctagtg ttggtgacgc cttctgttgc caatactttt tcttttttct
     1981 aataaacgca aacagacacc ttaaaatcct ttagaataaa gtcgacgaaa accattcaaa
     2041 ggagtgtttc aagatgtcct attcagagtt taccaaagag ttactagata ttctagacct
     2101 aaatcttatc tttcatgaag atgcctttcg caaagagcga atcaatgacg aaacatgttt
     2161 tgtttttgat ggcacattga cctatcaacc ggaggaatgc tttcatcgcc actatcaaaa
     2221 tgaacaaacg atcattaaat agggctggaa aaaagtatcc atcttattga atgatgtcag
     2281 taactacaag accattctgc ggattaacaa acagcgcttc aaatgtaaac actgtggaaa
     2341 gactttttta gcagaagatt cagttagtga tcgtcgctgc tccattgctc gcagagtcaa
     2401 acaagccatt cttgagctat taagcgaacc tctttctatg tctttgatcg cacgtatgaa
     2461 gcacatttct ccgaccactg tcattcgaat acttcgcagc cttcggccta aaaccatttc
     2521 tttgagccag ctgttaccag aagtggtgtg tttcgatgaa ttcaaatcag tgaaaaatgt
     2581 ctcgggagcc atgagctttg tcatgatgga cggcaagacc catcaactga ttgatattgt
     2641 ggaaaatcgg caactcaatc ctttacgcga ctatttcttg cgctacccta taaaagtccg
     2701 tgaacaagtg cgtctagtgg tttcagattt ctatacaccc tatcgcacat tggtgaaaga
     2761 gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat attagccaac acattggtcg
     2821 ggcttttacc aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg atcgtcggtc
     2881 gaagcactta aagaaatact ggaaactact tcaaaagaat gcgtgggaat tgaaaggtca
     2941 gcatcgctac tggcgtccgt catttcgaga tcatctgaca gaagcagaga tagtcgatcg
     3001 gctattatct tatgatgatt ccttaaagcg aggctacgaa gtctaccagg attttctttc
     3061 cgttatcaga agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag actacaagga
     3121 gttgccagag cactaccaac cagtgttcac gaccttcaag aaataccaaa cagaaatcaa
     3181 acgagcgttg cgcgttccct attccaacgg ccctatagaa tgtttgaata accatatcaa
     3241 agtactaaaa cgaattgcct atggctatag aaactttcaa aactaccgag aacgaatctt
     3301 tttatatcga ggaaaatatt ttaagaaaac taagaatact acccaactga ctaaagccag
     3361 aacaacaaca cggctagaca agatagctgt ctagccgtgt gagtatctcg attattctat
     3421 tgaatttctt caccaacact cattgacgaa gagccaaaat aagtaatcta accgactgtt
     3481 aactaaacca ttgcaggtcc cgttaaagat acttgtgcct gttgtcaccg cttctttttc
     3541 ttgtccacaa gcgcttaaca cacttaataa agcccctgct attattaata ataatatctt
     3601 tttcttatta aaaacctcct catctatttc cagattagta aatgtagtaa tctgtgcttt
     3661 cttttttctc aatactaata actaacttat ggggtaaaca aatactcgtt tgaccgggtt
     3721 ttgagatcca tccagttcta accgcgattt gatcaggact attatcttct ttaactcgaa
     3781 ttcttccatc tttaatctca acaatattat attgtccctt cgcaggatga taattcacga
     3841 gtttatgatt gatcttatcc aaatcgaagc gatccatttc cttgccatca attcgaacga
     3901 tggcatattt gatagcatct tcagttgttt cattctcctt ttctatagag aatacaaata
     3961 agggaataaa agaggtaata atcaaaatgc caataattat gacatcgcct atcttgacac
     4021 cagcttttct taaaaatttc attaacgata tctggctaaa catattagac tttttccatg
     4081 gaaatggtga aatttttcgg aaattatctg cattaagaga atcagttaaa caaccctcaa
     4141 gttgttcctt aatacattgt tgatccatct tcataccaat aaaaactagt ttattaatgc
     4201 gttcaccaaa caacggatgc caggcttcct tcaattcggg atttttttgt ataatcattt
     4261 gacactcaat tttaggtaaa ctagctaccc aataatttaa tgattcaata cttttatagc
     4321 tacccgatgt ttcaaagatc gtcgaaaaat cactatgttg ggcaaaccaa agaaaccctt
     4381 ttgctcttgt aatattgcca ggaaaatttt tatccgtaag atgggcaaat ttttgtgcgt
     4441 caaggagtaa ccgatttcga tagataaaac ttgagatacc atattcttct gtttcaggaa
     4501 tatgattttg atagccaagc tctaactctt tgaaccagcc cactgaatag gaagtctcat
     4561 ctatattaaa aagatttgta tttagaattt tcgataagtc tacttcacct ttaattgtcc
     4621 taattatttt ggcattggac tgtaacttag caattagacc gttaattttg taaatcgtct
     4681 tggttgacac taagtcacta ttatttaaaa gtaatatgtt acaacactcg aattgatcaa
     4741 ttaacaagcc acgaatatcc tgttcttctt caactacatt accttcagct tttctttcct
     4801 gaatcttttc acccgtttcg aaattatccc aaaaacgttt tacatccact actataataa
     4861 tggcaccaat ctctaatcgt tgggtcaaat caatgtctaa agggttttta gctaatataa
     4921 ttgcttgaat aattggaatt ggtttgctga ttcctgaagc ttcaataaca atttgattca
     4981 agtcagaaaa ttgcactaat tgatctagtt caacaatcaa atcttcgcgt aatgtacaac
     5041 aaatgcatcc attcggcatc gaaacaagtt tttctccagt tcttttaaaa cctttcttgt
     5101 cgactaatac cccatcaaca tttacttccc ctatgtcatt tacaatgaca gcaatttcct
     5161 ttccgtgatc accgtacaga atatgattta gaagtatcgt ttttcctgaa ccaagatagc
     5221 cagttaagat tgtaataggt atttttttca tagggaacct ctttctatac cttgaattta
     5281 cactttaagt gcaacacata aaaatgagtc ataatcgata tccaactagt ataagtttgt
     5341 cgagaacact cctagaagga aaataattat gagccagtat cgtcatctta ctatactcga
     5401 acgagaaaga atctttctgc ttcacgaaga aggattttct attagtataa ttggcaacga
     5461 cattcagcat agcccgttta ctattttaca tgaactgaga agaaacaaga aaaaaactat
     5521 tcaccttcca aagcggaaaa aaattatgct tatcgaagaa aaaaatgcgg acgttatcac
     5581 attctaaatg atccgtgagt ttggtcaatt gttcgtcatt tatttgtcga ttttcagtgg
     5641 tcgccagagg aaatctcgaa tcgtttgaag catgaacata tggacttttc aattagtttt
     5701 atcacgatct aacgtgcaat ttgcaatgat cttttcgata cagaacccct ctcacacggc
     5761 aataaaggcc tgatccgttc attgcaccat cgaggaaaaa caagacatac aaaaaatcat
     5821 gtcgagtctc gtggaaagat ttgccttact cattcgatcc atggccgtcc aaaatccgca
     5881 gatgatagaa gtgattttgg tcattggaaa gccgatattg tagctggcaa aactggtggt
     5941 acttgtttag tgactttgac agacagaaaa tcatgcttcc ttttagcagg aaagaaggtc
     6001 cccagaaaga aagccgctta tgtgagcgag aaaatgattg aacttttaag cagtattcca
     6061 aatgaggcgg tcaaaacgat cactccagat agaggaaaag aattctccaa tcatttcgca
     6121 gttacagaag cattgaataa tatgcccttt tattttccag atcctcatgc ccttggcaac
     6181 aatgaacaaa ttaaaatacg aatagactca ttcgagaata cttgccaaaa tcgaaagaga
     6241 tagctacggt agataactca acgataaatc agatgattga aaaactgaat aacagaccta
     6301 gaaagtgttt agattggaga acaccttatg aagtcttctt tgataaagtg ttgcgcttga
     6361 tttgacaatt caagacataa taaaaagcag atcaaatcca aataagggaa aaatgatcta
     6421 ctctggtatt atcaattaat tggccaagct attatgccgt tttgctaaac gtctacttct
     6481 tccagtattg accttgataa aacaacaatc aggatcccgt tttaatactc tatgaactgt
     6541 gtcaatacta acatgtaaag ttgcagcaat atcagctaat gtactcccat ttaatctcat
     6601 ttgctttgca aatcgataac gccgataagc atcaatattc ttggaatcca tagacaataa
     6661 tacttttctt accatgtatt tcctccaaaa attgaatttt aatgagccat catttcttga
     6721 gcaatttcat gtttagacaa tgaagctgga tagaaagtag gccaattatg caattcatta
     6781 tagagttttt cttgactttc tccaccaaag aaaatatgga aatgctctgc cttacttggt
     6841 gcaattccat gatcactaaa ttgaatatat ttaaaacgac cagcacctgc atcatcagtt
     6901 tcaaaattaa aacgaacacc acggtttcct ttttcataat tcaaaatttt gtatccaaca
     6961 tatttgtatg tgtattgatg atgttcaccg ttgactacaa aatcaatggt gttgtcttta
     7021 atgttgatag tatcaatatc tgtcttatag ccagtagtat aataatcttt gtactcggca
     7081 gctgtcatgt ctttattgat ttttgctttg taatcgaaaa cttcatccaa aattccatct
     7141 tttaacaacg gataaacaga ttgccattcg cctgtataat cactcaattc gcgatccttc
     7201 acatcggcat ctttaaagta cccattagca actgtctttt catccttcgc ttctttttct
     7261 ggaactacct cttcaccagc aaccatagtt gttttttcta acgcttttag attgtcttcc
     7321 attactgaaa cataatcttc accattatcc atttgttctt ttgtaagact ttcaagagga
     7381 ttcagtactt ccaattttat cccagcttcg ttggccaaag tttttgcaat tttatcatta
     7441 gcattctttt caaagtaaat ataattgatc ttattttttt taacatactc ttttaattca
     7501 gctaatcgac cagaagatgg ctcttcttca ggacttagtc cagcaatagg aacttgtttc
     7561 aatccataat ccaaagctaa atatccaaag gctgcgtgtt gcgtcacaaa acttttttgt
     7621 ttcgcttctt taagactagt ggtatagtca gcatccaacc gtttcagttt agtcaaatac
     7681 ttttcagcat ttgtctcaaa tacttttgct tttttcggat acaatttaac taactgatcc
     7741 ttgatatttg aaacttcctt aattgccatt ttgggtgaaa cccacgtatg aggatctagt
     7801 tcatgatggt gatcttcttc tccgtgatca tgatcttctt cttcactacc tggcaataaa
     7861 atcatgtctt ttgttccttc tactacatta ggcccagctt tcttccaact ttctttagct
     7921 tttggtaccc atgattccat gttttcgtta tgatatacaa aaacatcagc atcatgagct
     7981 tttgccatat ccttagctga gggctcatag tcatgaggtt ctgatcccgc tggaaccatt
     8041 aaatccacgt tcgcttcatc gccgacgatg tttttggtga aatcatacat tggatagaat
     8101 gttgttacaa tttctaattt tccatcttta tcattcgata cttttgcatc attagtgctg
     8161 gagcaagctc ccaataatcc tagagctgct atcccaataa gtcctacact gattttcttc
     8221 attctttttc cttccttaat tatgctaatt tcttcatttt atttcgttta tgttctttga
     8281 taatttttaa tgcccttttt ctagttttaa tattcgggct tttaagtcgt cgatatgcac
     8341 tagatatatc cgttttctcc attagaaatt acccccaatc actacttatc attatttgct
     8401 ttccgccagt aatttatcaa aactctgttt gatcctttct tcatccaaca tttttccgat
     8461 tagaataata gtggtttggt ctttttgtcg agtggtgtat tgaaaattaa cctgttcgtt
     8521 tacctcttgc atggcaacta taaaatcctg ctcttgtaaa gcaatcaatc ctttaaaacg
     8581 atacaggctc ccttggttgg ttaaaatcag ccagtcaatc cattggagta aaaaatcttt
     8641 ttttatgtct tgatctgaag ttaagagaat tgatttaaac tcatgtactt gatggtgttg
     8701 atgatagtat ttgccattat agtgattttc tgatgaatca ctttcaaaaa ctaagggatg
     8761 attaaatttt ttcaaattat aaaaatcctt tttttgaatc gtttcactat gtgtgaacat
     8821 tcgaatatcg gataaagggt taatactttt gatttctctc tgtaattttt ttagtgcaga
     8881 taaattcaag ttttgttttc tagacacaaa aatgcgatct gccatgacaa gttgcttaag
     8941 ggcttgtggt tctctttcaa taagttcaaa actgtcacta tcaataacag tcaataaact
     9001 atcgatatat actcttcctc ttagttgagg cgctgttaaa agtgtttgaa tgattggttg
     9061 gggatctgct atacctgtag tttcaatgat gatttgtgaa attggttgtt gatgttcttc
     9121 tattgtttcc agtaaagaaa tcaatattgc taataaatca ctgcgaagac tgcaacacat
     9181 acatccatta tttagttgta acacccgttc ctcgctatga actaataatt tgtgatcaat
     9241 cccaacttca ccaaactcat tttcaattat aataatttct tccctggggt agggtgagac
     9301 ttgcaaggct tgattgatta gcgttgtttt tccagcccct aaaaacccag aaacgattgt
     9361 aactggtatg cccattttgt caccttttta ccacttagct atttcacttc tttaaaaatt
     9421 gcccgtctgc gcaattttgg cgaatacttt tttaattcta accgttcagg gttattccgt
     9481 ttatttttgc tggtgagata cagacgttct ccagtttcga cacattccaa aataatattt
     9541 tgacgcataa atcttcctcc ttcaatactt atcgatcttc aagatatgct tgaatttgtt
     9601 gtgcatcttc aaaagttaac tcgccagatt ccaattgtga aactaaattt tctttcatat
     9661 caacaggtat ctcccaataa ccatcgatac attgaaataa ctcctcgtca tactcttcaa
     9721 aaattggata gattcttaaa aaatcatcaa atgataagcc ttggtcctca tagtaatctt
     9781 tgactaatcc attttcaatt gcctgttggg tcggtaaacc cgtttcagta ataattcctc
     9841 tctctaccat cgaacgataa aaagtgcatt tatctagcga ttgctgggtc gtttcaagat
     9901 aatactttaa tccttcttca actaaaggtg ggtaaataat ttccattttg attccctcct
     9961 aaaagttata tattaaacta ttttgaagct cctttaatta ctttctttcc attatagtac
    10021 ccttttaaag atacatgatg gctacgtcta taatcaccat tcgcttcgtc aaatgaaatt
    10081 tctggtttta ctacagtttg atgagttctt cttaaccgtt tcttcgcctt agatgttttt
    10141 cttgctggta ctgccatgat atttcctcct tttaccaact tgcttttttt actccaggaa
    10201 tcaacccttg atgggcaagt tctctaaact taatacggga cataccgaac tttcgcatat
    10261 accctcttgg acgaccatcc gtctgatcac gaagcctcag acgattcgga tttgaatctc
    10321 ttggcaattt agcgagagcg gtgcgatctc ctgcggcttt taattcctga cgtttatctg
    10381 catacttttc aatcatcgcc atttgtttct ttgctttagc aattttcgac ttttttgcca
    10441 tataccttta tactcctttt attttgattt agacacctca aatcgtaatc gttacgtttt
    10501 gacactgata ctttaccaac aagagttgct cttgtcaaca ataaataaca tgaaataaaa
    10561 ctcatatgaa tagataacta tttgaaaacg aatttatcgg tacatagaga taatcaaatc
    10621 atttaaaaga atatgcagtt taggctctga aaaataagaa ttcactttta ttagtccaat
    10681 caaacacaaa taccgtttta agtagctttt cctctactta aaacggcaat atgaaagaaa
    10741 ctataacttt ttattcaatc ttttatgttt aactggttta tcgccaattt tttaaaactt
    10801 tgattattcc ttatattata agaatagaca ttaagtgaac taattcaatc atactgattc
    10861 tttcgtgtat tcaaaattag atgctgttta ataaaattat tctttttgaa aaagaattat
    10921 aactatatcc caaaaattgt aggttaattt aaagcctatt tcatcatcgt gattgagctt
    10981 ctttttcata gacgaatttc tcagatagtt tagttttctt tatgagattt ttgtatattt
    11041 ctgaccttcg atacaactca ttatgagtac cttgatcaac tagttttcct tgatccatca
    11101 ctacaatttt atttaccttt tcaatagact tcaagcgatg cgatataatt agtactgtct
    11161 tatcttgtat taaacgattt aaactttcct gaatacgttt ttcattttcc gcatctaaac
    11221 tggcagtaat ttcatctaaa attaaaattg gcgcattttt caaaaaagcc cgtgctatgg
    11281 ataggcgttg tcgctctcct ccagaaagtg aacttccgtt ttctccgacc aatgtatgat
    11341 aaccgtcagg tagtttttca ataaagtctt cgcagttagc taaacgagct gcttttttca
    11401 cttcctcatc tgacgcttga ctatttccaa tacgtatatt ttccaaaata cttgtattaa
    11461 acaaagttac ctcttgaaaa acaatagcga ttttgctaaa caaagaagct ggagatacac
    11521 gtttaatatc atacccatca attaaaatac agccttcatc ataatcatac agtctagaca
    11581 ctaatttcaa gatactagtt ttcccggaac cactagcacc tactaacgca gtcacttctc
    11641 cttgtttagc cgtaaaagag atatgatcta aaattggggt attgttatca tatgagaaag
    11701 agacatctcg caactcaatg tcaaaagatt tcagaggact atctgatccc tcttggatac
    11761 tggtttcctt catagctcta attcgctgta ttttaggagc taaataaaat atttcaagca
    11821 ctgcttcttt catagaatcg aaggaatcct ttatttttat tgcggctagt aagtaaccaa
    11881 cgacgtagag tatagtcact tcacccgtta acagtaagtg aacacctact agaatgacta
    11941 ctgctaaact aacatatgag aaaatagagg ataaggccat gactgaaaaa gtacttaatt
    12001 ctacctttaa atgaatcctt tcactctctt ccattttttt gaaaagtctg tcttgaactt
    12061 ttttagatag attaaagcta ttaatctctt gctgcaattc tatagtttct tgaaattctt
    12121 ccgagttttc tctcaaagta tcgtaatatt tagtattggc tttcgtttgg gatttctttg
    12181 ataacaagat taagacaaaa ctaaataacg ttggcaaaat aacagctaat cccattttga
    12241 cattaccaac caaaagcatc actgaaataa aagggaaaaa cagagccata ccgccggctt
    12301 taggtattgc atgactcatc gcatgctcaa taccttcaac gtcagacatg atagtttgag
    12361 ataaatctga taaattatgt tttgaaaaat aggataatgg taaatttgac aaatcgtctg
    12421 caatttgcac tcttaaatgc gcactttctt tataagtact attatataag ttttcatact
    12481 ccctatctaa caaccaatac atgaaaagca aagtaacaaa cgatacggca aaataaagcc
    12541 aacggggctt tgtattttct aaaactaatt cgtctatcag catcattata atgaatgctg
    12601 gtaccatatt aatgcagtat acaaaaaaac tagaaataga agctttagtt aaagcttctg
    12661 cgccctgatc acttaaagca aatttgtttt tataaaaact tttcattgtc aagcctccaa
    12721 tcatttgcat tctcataatt agtgaccaat tgtttataca cttgatgttt agccattaat
    12781 tcattatgat ttccacgttc tactattttc ccttctgaca agacaagaat ttcatctaca
    12841 gatttaatgc tggataatcg atgagctatc ataataactg ttttttcttt catcagatgt
    12901 tgaaaagcct tttgtagttc atactcatta tctggatcaa tagatgcact ggcttcatcc
    12961 ataataacaa tctttgcatc ttttaaaatt gctctggcaa tagctatacg ttgtttttct
    13021 cctcctgaca gataaactcc tttactacca attaaggtat gctctctttc gggaaatttt
    13081 tctaaaatcg attcgcaacc ggctaatctt agcgcgtcaa atactttact tgctggcgct
    13141 tgaggatttg ctatagaaac gttatcgtaa atactctgat tgaatagttt cgtatcttga
    13201 aagacaaaag caattgtgtt aataattgtg tcttttgaat agtgatttaa aggcttacca
    13261 ccaattttaa tctgtccctt atccacattg tagaaacctg aaattagttt agctatcgta
    13321 ctctttccgc ttcctgattt tcctactaat gcataaattc ttttttgttc taaaactaag
    13381 ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc tgacattttc aaatgcaatg
    13441 tcacaatttt taatctgtgt ctcgttccca tattttagtt ggtcttgctt catttctaaa
    13501 taaagggagt ctagtgtttc gactgcatat cttgcttcaa acaagtgcat acttacatac
    13561 attattctca tgaaagaaac aaaaataatt ccacttaaaa aaaacaacat caacaagtca
    13621 acagctaata tcttgggagt ggtaagagaa gttataaaaa aggttatagg aactatcaaa
    13681 atgcaaacta tgccaaaaaa tagccattga aaaagaacaa aaggtgtttt cccactcaaa
    13741 gaatacttat atgcaaactt agcataatcg ttaattgctt tattcaaggc tttaaatgat
    13801 gttactttag caccaaatat ctttataact tgaattccgc ggatatattc taccgtttca
    13861 gagcttaaca catctaacga ttcttgatat ttttgaataa aatttgtatt ctctgtcatt
    13921 ttctttaaca agaatacacc aattactaac atggcgatta gaaccaatcc aattttaaag
    13981 ttaatgaaaa agcctaaggc aactgataaa acaggtgtta ctatggcttg agcactatct
    14041 ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt tgtcatcaat tgtctttcga
    14101 atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg cttctgttaa accgctaatt
    14161 cccttttttc tcaaattagt ctctaagcga aacccaagga tatgagaaaa taagccagaa
    14221 aaaatataaa ataaagctct aaagttaacc aagcaacagt ttgaaccgca taactcttag
    14281 ctaaatattc atctcctaca ataattagag actgaagaaa tttgtaaata ctgtaatatc
    14341 catagactgt taatacgaca gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt
    14401 tcattttagg gacataacta aataattttt tgtatacttt cattttttcc tcccccgcat
    14461 tcaatttctt ctcttattcg tttacaactg ttaatgcttt ccgtaatagc gtaaattaga
    14521 gatgtacaaa cagataaaag gtaaactacg ttctattttt tgtatttttt taagactttt
    14581 cttccaatca atacaccaat catagataaa cttgctacaa ctaacactga aatgattccc
    14641 catataccta ccgcatagtg gatcattgca tccattgttg cagcagattg tcctttatct
    14701 atccaatctt gacgaaaacg atctgtaaag aaccatatag gaatccactg tccggcagca
    14761 ctgcctaatt gcattagagc ccaagacgca acttgaggaa ttgctcgatc atatccaaat
    14821 acagatagaa taatatcagc taagatcgcc atactaatcg aactgataaa ccaaggtaag
    14881 tacgccccgc ccattaacgt aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt
    14941 ccttttctag ggtagttata gctaatataa acaaatatga ttccagataa taatccaaaa
    15001 atcccggggg atatgctgtg accaaacgcc ccaccaatac tactaaccat tattgaaaaa
    15061 aagtaaatta ctagggaaat aactgtagct aaaccaataa aaatataatc ttttgttttc
    15121 atatatattc tcctcaattc tatttcgtta tgtcattaaa aatctgttct atcaaagttg
    15181 aatcttctac tctaagtttt atatcttttt ttattccacc attttttaaa taaataattc
    15241 tattgcatgt tttataaaga aattccggat catgagtagc tataactact gcaccctttt
    15301 cttttaagtc tttaataagg tcagcaatcc tttccatatt aatataatcc aacccacttg
    15361 taggttcatc aaatagaaat aaattagttt ttgacaatga agctatagaa accaataatc
    15421 tttgtttttc tccaccggat aaatcaaacg gtaattgatc tttcatttta taaagttcca
    15481 ttttttttaa aattttttct tgctgatctt tactaactat ttcattcccc agggctaact
    15541 cgctcctcac tgattctgtg aaaaattggt aatccatttc ttgcattact aaaaacggcg
    15601 atgacaaatt ttttttattt tcgattatct ttcctttggt agggcgaata atcttcatga
    15661 gtattcttag taaggttgtt ttccccacac cgtttttccc taccaaacca ataacatctc
    15721 caactttgac atcaaaattt atgtctttta gaatattttc ataagtaatt tcttcaactt
    15781 ttaatacatc tgcacttacc ttttcgatat agggaacttc aatcgaacta ttaaaaatgt
    15841 caaatttcct taatgcgtta ttgttttcag tttttaacaa ctgaatagtt gtagttttta
    15901 cagttctatt tttaacaaat agaacttgat caattaattc tttcaaatag taaaaacgat
    15961 gttcagctat aacaatagta aatcctttat tttttaaatc acaaagtaag tcagcaagtt
    16021 tcttagtcgc tttgaagtcc aaatttgaag acggttcatc caatataatt aatttagagt
    16081 ttagcgataa actacatcca atagaaattt tctgccgttc accacttgat agcgaaaaaa
    16141 tgtttttatc taatatattt gaaagatcta ataattcagc taattcattg attcttttat
    16201 ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat
    16261 ttgttgtaaa gaattgtcct ctaggatctt gacttacgta gccaatattt tttgatatac
    16321 tttcttgtgt taaggattgt agcgagttat tttcaaataa aatttcccca accaattttc
    16381 cgtcataaag tcttggaata agtccattca ttaactttaa tagtgtggat ttcccactcc
    16441 cagaagaacc agtaatcaat gtaagactgc ctttttttat tgaaaaatta aggtcaacaa
    16501 gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt gttaaattca aacaattctt
    16561 tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg aaaacaggat gatatagtct
    16621 aataagctca tgtttgtttt atagtaagaa cttcttttca caggagattc tatccccttt
    16681 gttattgcat tagcggtcat ttcttcagca atcatagttg cacgtataag aacgggaact
    16741 aacagaaatt ccaaatattg cagaggctga aaaaaagaca agcgaatgtt tctcattttt
    16801 aaagattctt taatcattct tatttcagaa cgataagtcg gaaagaatct gatcactact
    16861 gtaatactta acattatttt tttcggtaaa tgtatttttt ctaaagagga taataattca
    16921 ttgctagtat agacactaac caacaaccat gagatcatta cgaccggcaa cattcgaaat
    16981 acaatatata aggatgtcgc aaaaaatata gtaaaaaact ctatcttgat agcatcaata
    17041 attttcatta ctaaaaacag aactgaataa atcaagatga aataaataca tgtctttatt
    17101 ttacgaaaca tcaatactaa aactgtagca ataatgaaac accccacttg aaatatattt
    17161 ccctttccta aaaaaataat gaccggaaaa aaaatattta aagtaatcaa ggttaacgga
    17221 ttgatttttg acactctttc acctccaaaa aataagatcg cactgttatc ttaagctgat
    17281 aaaaatgcac cgttattcag ataacagtgc gatcttatca aaaagcataa ctgagaacaa
    17341 ttctcaatgc aagctttttt taattaactc agtaattcaa tccacccagg aacagaaaat
    17401 tgatacaatc ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata
    17461 tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact
    17521 aaattttgat tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttc
    17581 gtcacaaatg atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat
    17641 aaaatcaaat cgaatatacg tttattctca aaaatataat taataaatgg atctaattca
    17701 ttgaagttct ctctccaact tttctctata tctatatttt ctaagtttaa gaggtatttg
    17761 gtaaactctt cattatacat attaataagc gcattaatat gcggctgaat aatttcttca
    17821 aataacgctt ccttagaagt aaaatgttta taaaaagcac ccgtagttac gcctgcggtc
    17881 aggcacagtt gccttatctt agtttgctga aatccttctt tttcaaaatg gcttagtcca
    17941 ctttctatta acttttggtg agttataata taatctctgg tttctatggg tacttcctcc
    18001 taataagatg aattaacttt attatcttac acaggacttg aagtttcaat cgttaacgtt
    18061 tattactgaa ctcattcctt agagctaaac taatgagaaa gagcagaata actgatacaa
    18121 aaagcaatcc tacaagaatt aatagactca tctaaatcat tcaccttttc taaaataatc
    18181 cgagaaagat ttaaaaaaat tatatccagc tattaggata agtcctatca taatcactcc
    18241 aatagccatt ttcttatcct ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa
    18301 tcgtcaataa attttttccc tttatcagtt aattgaaatc ctatatctaa atagtcctca
    18361 tatctaagtt tagccagcat ctcacttaca gatgctggac taacacttag aacttttgct
    18421 aaatcattat tttttaattt cttttttctt tttgataatt cctgaattgc tagtaaataa
    18481 attagtatag tagtcgtttt aatttgtacc atacctaccc tccaaattat ttcgaaaggc
    18541 cttcagcaat tttatccagg ttccatttca tcatcgcata gtaactatca ccatcttgtc
    18601 ctttttctgc aattgaatca gtaaaaatcg ttgaatagat aggaatattg gtatcttttg
    18661 atactgtttt cattggtcta tcatccacac tgctttctac gaataaggag ggaacttttg
    18721 ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg tgttccttct tcttcagtgt
    18781 tgatttccca aatgtaagca gaaggcacat tatacgcttt cgagaaatat ttaaagcatc
    18841 cttcacttgt aacaatcatt tttttatcat tcggaattga agcaaattta gatttagctt
    18901 ctttgtctag agaatccaat ttttcaatat acttatctag attttcttta tagaattttt
    18961 tattatcagg atctttttcc gctaactgtt tttcaatatt tttagcgtaa ataataccgt
    19021 tttctaaatt taaccaagca tgaggatctt ccttcccttt ctcactctga ccctctaagt
    19081 aaataacatc tatgccatca cttgctgcaa aatagtcttt gttttcctct ttgttcgcat
    19141 ttttaactaa ttttgtaaac caagcatttc ctccagtttc caagttaaca ccgttataaa
    19201 aaatcaaatc tgcttttgaa gttttttgaa catcttcagg caaaggttca tattcgtggg
    19261 gatctttccc aataggtacg atactgtgta aatcaatttt atcttttgct atattttcgg
    19321 taatatctgc taaaatcgaa ttagtaacta ctacttttaa tttttcgttc gaagccactt
    19381 tatctttgtc tttactattt attgaacatg ccccgaatat aaaaagagca atcaaaatcc
    19441 ccagacttcc cagtactgct ttccattttc tcatcataac gatctctcct tctttcttaa
    19501 aaatttttgt tttggtgaga ttagaaaact aattacaaat aagaatgccg ctgttaaaac
    19561 aatacttgat cctgctgcta tgttaaaact ataaccaata aacaatccaa gcaatgatga
    19621 acttgctccg actgttgcag aaataaatat catagttttc aagctatttg catataaata
    19681 tgctgtagct gctggtgtaa ttaacattgc aacaattaat atagttccca cactttgcat
    19741 agctgttact gctactaaag ttaatagaac catcaataga taatgataaa aactgacatt
    19801 catccctatt gcttttgcca ttaatggatc aaaagaagtg attaataatt ctttaaaaaa
    19861 tagaactact ataaccaaca ctaaaaccga aacgccaatt gtaatccact tatcaatatc
    19921 ttgtacagct aaaatatttc caaataaaat atgaaataaa tccgttgagc tatttgcaac
    19981 tcctataagg ataactccta gtgctaaaaa agaactgaaa gtaataccaa ttgctgtatc
    20041 tccttttatt acactattgc tttttataaa agtaataatg atagaagcta atagaccaaa
    20101 tacaatcgca ccaataaaaa aattaatacc taaaatataa gaaattgcta ctcctggcaa
    20161 aacagcatga gaaatagcat cacccataag agacattcct cttaaaataa taaaacaacc
    20221 tactgttcca gaaacaatcc ctataactac agatgtaatt agagcattct gtaaaaagtg
    20281 aaagtcatac agaccattaa taaaattttg aagcatatta ttccccctct ccaataaata
    20341 tagtatctcc ataggctttt tttaaatttt cttcattaaa tacagattct acacttccgt
    20401 gagcaattag atttcgattc aaaagaataa tattatcaaa atacttcttt accttgctta
    20461 aatcatgatg cacaattagc actgtttttc cctgatgttt caattttttt aacgtatcca
    20521 taattattcg ttcactaact aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat
    20581 ccgcattttg agcaagacat cttgctaaaa gtacacgttg aaattgtcct ccagataatt
    20641 ctcctatttg atgattagaa tattttccca tatcgacctt ttccaaagcc tttgaaactc
    20701 tttgccattc tgcattttta attctttgaa aaactttcat tccggcatat gttcccatag
    20761 atacacattc ttttactttt attggaaaag taaaatcaat atcagctttt tgctctacat
    20821 atgcaatctt caccagtttt ttattgatat cttcattatt aatggagacc tctccctgat
    20881 gagggataat atttaacatt gctttaagca atgtggattt tccagcacca tttggaccaa
    20941 taatacctgt taaagcacct tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat
    21001 cgttataaca aacattaagc tttttcactt ctaacataac tttctccttc taaatgataa
    21061 tgattatcac gatcatatgg tatacaaaaa ttatatttta gtcaacccta aattttatag
    21121 tttagatttt tttctgttgc tgaagattag aatgacaaaa ctatttgaaa ctacgcgtat
    21181 agtaagtagc tttcaaaata tataaaaaag gagcgttctc aaatgagaac atgctcctaa
    21241 aactacttta ttggcgtatg gaaaaattat acttgaagtg gatagcgggt tgaataagtt
    21301 gataagcatc aaaacattta taaaaattat tcaagactat gtctgttcca tatacaaata
    21361 tgaaaatcag taaactccat ttaacttttt tcgtagtgtg tctttttata catgtttatc
    21421 gttattaata gtgaatagga aaaaaactat ttttgatcat tgacaattaa atacaatttt
    21481 tgaataacga tccctgctat tcgagctaga aactcaagta cgccatgacc tgattagcga
    21541 caaatactta gagatttctt tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa
    21601 ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt
    21661 acccaaagca attcaaaaat ttcataacta ctattttagg agatgatttt atagaatcca
    21721 gagtctacct gaataaaaaa caaacctgtg catttctagg aatcgataat aatactctag
    21781 ataagtggat tcgtatatat aatttcccag taataagagt tggtggtgta gttcgctttg
    21841 acgtagaaga tattaaagac tttatgaata catttaaaat aacttgaaat tttcttctcg
    21901 ccgcatatac taaaattagt gaaatatact gccttacttt ttttaaggag tgaggatttt
    21961 gcctacaaag ctttcaaatg ggaaatataa aactaatctg agatatccaa aaaggtttaa
    22021 agaaataaca ggaattgcat cagaaaagta tcaaaaaaca tttccaaata ggcaattggc
    22081 gattaaagcc gaaaatgata tgaagaagaa aatagaaaaa gttttgagag aggagaatgc
    22141 aaactcatta gaattaaaag gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct
    22201 tcctagatat gaattaggtc aaacaattag atcaaacagg cctcctagtg atataaccat
    22261 tagtaataca aaagatatat ttcggttgca tattttacct atgtttggtg aatacgcaat
    22321 gaattatcta aatatcaata ctgagataat atcagatgaa ttaaccaaaa aatcgaaaga
    22381 atatgcaaat ataaaaatca taaaaggata tgttcgttcc atgttcgata ttgcagaaat
    22441 actaaattat atcgaattta atcgaactac taaaattatt caaagtatca ctgcacctaa
    22501 aaaaaatgct ttagaagaaa aaagaataca agaaggtaag caagcactat catcaaaaga
    22561 acttactaat tggatagaag cagtaaatga tgattttaat aatcatttac ttacttttca
    22621 tgattacaca ctttttatga ttaccctgta tcttggtgat agaaaaagtg agacttacgc
    22681 tttgcaatgg aaatatattg attttgaaaa acaaacggtc cgtcttaaac atactttaga
    22741 taaatatcaa agaaaaaagt ttacaaaagg tcgaaaagat accgtcattc aagtaccaga
    22801 agttgtaatg acattgctaa gtgaatggaa atcggtacaa gctgatcaat tgttgaaatt
    22861 aaaaattaag caaacgttag atcagtatct ctttacttat actaaaccat ctggagaagt
    22921 aaattgtcct gttcatgccg attacctaaa ctatcgaatc aattcaatta aaagaaggca
    22981 tcctgactta gttcatttat ctccacacaa actccgtcat acttatgcaa ctatagctag
    23041 acaaggtgga gcagatatga atcaaatttc aaatgcttta acacactcag atatttctac
    23101 aacaaagata tatgtgaaca caccagatat agttgataag gctgtttttg aagcttttca
    23161 gagaggattg aataagtgtg attgatatgt gttcttaaat gtgttcttaa aaacaaaaga
    23221 agcactttcc aaataattga aaagtgccag aaacgttgat atataaagga tattaacgtt
    23281 ttgagaattg tgaagcttta cgtgctttct taagacctgg ttttttacgt tcgaccatac
    23341 gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca ggatcaactt
    23401 ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg tatcctccgc
    23461 cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg aaaggttgat
    23521 tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt ttatttacag
    23581 taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta cgacggcctg
    23641 tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag tttgtgatgt
    23701 ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat acgttcaatt
    23761 ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca gaagtttcga
    23821 tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc aatcctcctg
    23881 ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt aatttcactt
    23941 tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt
    24001 tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag
    24061 ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata tatgttgtac
    24121 gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga gtttccgggg
    24181 ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt caatgtcaac
    24241 gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt cttttatata
    24301 aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac
    24361 aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    24421 tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga gctgaacggc
    24481 aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat tagctcaaaa
    24541 aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc
    24601 tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt cgatctctgg
    24661 cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg ttttgaattc
    24721 gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg
    24781 gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt
    24841 acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt cacgcaatgt
    24901 agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta actgagcgtt
    24961 caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg gtcctctcat
    25021 gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa ctaattgatt
    25081 taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt ttcttacact
    25141 taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg agaacgattt
    25201 tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa ttcttctcgc
    25261 agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac aaataatgga
    25321 tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc tttcaattct
    25381 atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca gcctaaaatc
    25441 acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta acccagtaca
    25501 tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc acggatctct
    25561 gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag acagatccct
    25621 tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc gataaaactg
    25681 ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc
    25741 agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc atatggattt
    25801 tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca
    25861 aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg atccagaaca
    25921 taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa acgaagtgct
    25981 ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa ccctagatta
    26041 gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa acctagttct
    26101 gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac ttgcggcaat
    26161 tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa ttcatggata
    26221 tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc aaacaatcta
    26281 taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa ttcaggttgg
    26341 ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc
    26401 tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc gtccgctgct
    26461 cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc caacaatatc
    26521 aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga aggcaactgt
    26581 ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg atcttgttct
    26641 gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg
    26701 gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa tccgattgaa
    26761 acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta agcttgcgta
    26821 tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg ttgctgtatc
    26881 ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat tatttatcct
    26941 agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat tccgattctc
    27001 tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc cttctactat
    27061 ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt ttcttccgca
    27121 agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt gatggattta
    27181 ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt atggattttt
    27241 tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt
    27301 gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt gatgctttct
    27361 tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc tagtctgttt
    27421 ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga aaagctgcct
    27481 ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta ttgaaaaaag
    27541 gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc aaatccttgt
    27601 gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca atcagctgac
    27661 cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt tccataagta
    27721 gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca
    27781 gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc ctgatggtaa
    27841 tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg cttatcgaat
    27901 cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca
    27961 ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc taatgcgtca
    28021 gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat aacttctgga
    28081 tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa
    28141 gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat ccctggctgc
    28201 tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc tttttttgtc
    28261 atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga taacgttttg
    28321 cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat aaatgccaaa
    28381 acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca gaaggaagat
    28441 ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct tgacaaccag
    28501 cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc tgtgctcctt
    28561 tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca ataaggacat
    28621 caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg cttgctgatc
    28681 ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt gcaccaggca
    28741 aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt ttttgctcag
    28801 caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat gacagcaatc
    28861 gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc tcagtgaatg
    28921 gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga gctgtgtcta
    28981 caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac ctccttgaaa
    29041 atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat tatcagtggc
    29101 ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat
    29161 gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag ctatcacagg
    29221 aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta gactttgtcc
    29281 atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt tatcgaactc
    29341 cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa atgctaacag
    29401 tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt gaggggcaaa
    29461 tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt ctttcaagcc
    29521 tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat tagtaacatc
    29581 agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt tatcaactgt
    29641 agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt gatcgtaaaa
    29701 agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac ttccgccaac
    29761 gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt ctcctactcc
    29821 tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca atccgacgat
    29881 ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt ctttgttttt
    29941 gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga
    30001 tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag cctgaacaaa
    30061 aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca gactttcttt
    30121 gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa gatcagttct
    30181 ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt cgtctctttt
    30241 tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa
    30301 ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag agttccattc
    30361 gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc taatgaagca
    30421 gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag gcacgcttct
    30481 tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat ggcgtgaatc
    30541 gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa attcttagaa
    30601 acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag atctgcttgc
    30661 ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat agactggctg
    30721 ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata cgtgtcatct
    30781 tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa aaaattttga
    30841 cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc cacattataa
    30901 tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc atctggacca
    30961 atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag ccaaaaagct
    31021 gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc ctctgttgct
    31081 tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc tgcggcagct
    31141 gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt gggtgtctct
    31201 tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg ataatctctt
    31261 gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt ctttgctact
    31321 tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc
    31381 cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg atcattgcgg
    31441 ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa
    31501 tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga catcgactgc
    31561 tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt cttaaacggg
    31621 tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa ggacgtattt
    31681 tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg gaatacacct
    31741 gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt ctggtaggat
    31801 gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg ctgtgtacca
    31861 aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc ttcggtcaac
    31921 ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc aacacgacaa
    31981 gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg tttaagctct
    32041 cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa
    32101 tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac taactcggct
    32161 gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct tacttgattc
    32221 cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat ggtcatctga
    32281 ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt cataaggtaa
    32341 taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg atgacatatc
    32401 cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc acaaaatgag
    32461 ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa accagctttc
    32521 catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga tcgccacaaa
    32581 aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc agttggatga
    32641 tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg aatttgcttg
    32701 gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg aagctgtacg
    32761 cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca agcatgtgaa
    32821 tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac gttccccagc
    32881 ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc ccgagaacat
    32941 caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat ccataagcca
    33001 aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca gctgtcatag
    33061 ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat acatgttcga
    33121 agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca ttcagcattt
    33181 gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct
    33241 ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt tgataataag
    33301 cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc ttcatttatt
    33361 tctctctcct ttgcttattt acttattgtt attctttctc tattctttct ctaaatcatt
    33421 taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct gataaatttt
    33481 cacttcattt tctcctcgca aacgacattc atataccata taaccttcat actcattttg
    33541 aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg
    33601 attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg cttttgaaat
    33661 atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat gtttcaattg
    33721 attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt cttggtatcg
    33781 gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat ctaaataacg
    33841 aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg
    33901 taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc ctccaacctc
    33961 gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc gttcttcgat
    34021 aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga tccctgtttc
    34081 tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt cgatttcgta
    34141 ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc ttgggaaaaa
    34201 tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac gcccattttg
    34261 atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct ttctgcaaat
    34321 ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa taattcccaa
    34381 catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg ttcactagat
    34441 gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag attaaaccca
    34501 gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc gatccctatt
    34561 aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc tactccacgt
    34621 ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc tagcgtgtaa
    34681 tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat cccaacgatg
    34741 gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc gcctaaaccg
    34801 aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc taaagcgaat
    34861 tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag tttacgtagt
    34921 ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata agtgcagact
    34981 aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc
    35041 atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc ttctcttggc
    35101 aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa tcctctaaag
    35161 tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg tacttcgttc
    35221 ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc taatgcaact
    35281 aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc cgccttcgac
    35341 taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg gtaaagcggt
    35401 caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt
    35461 caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag caaataaata
    35521 atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag aaatagccaa
    35581 tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca ttgctgcttc
    35641 ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg cagtaggaag
    35701 tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac ctagaccgga
    35761 aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa cggtgtaaaa
    35821 gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt atgccccgct
    35881 gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac tatttttgaa
    35941 atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt caaaagtcca
    36001 ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt
    36061 catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta
    36121 aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt tcgtgagagc
    36181 aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc ttttccaaga
    36241 tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac tttattcgtc
    36301 agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac aatcacagtt
    36361 gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca agcatttgca
    36421 ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat
    36481 gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat aaagaagcta
    36541 gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct atctactaac
    36601 agatcattga cgattccata actattattg aacatatatt tccaagcaaa aatcaaagaa
    36661 atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct aaatctgaac
    36721 ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc tacaactaag
    36781 aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact taaaatttca
    36841 agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg catatcgaaa
    36901 aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa taaaagcatg
    36961 cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg acgattcatt
    37021 gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac cattggcaga
    37081 acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac atcttcgttt
    37141 ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat atctcccatt
    37201 ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa agcactagca
    37261 atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta accatccagt
    37321 tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac ccacttttcc
    37381 atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg
    37441 tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg atctttttgt
    37501 gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga aggcaaaatg
    37561 taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt gatttctgtc
    37621 acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga caatgcttct
    37681 ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt tgctccattt
    37741 gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt aggcatagca
    37801 attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa aatatcatcc
    37861 caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata ccaaatacct
    37921 tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga acgaacaagg
    37981 tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt ttggactgcc
    38041 gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact gacttccatt
    38101 acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa cgctgattct
    38161 tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc agcaaccagt
    38221 tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg aatcgttgtc
    38281 tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa tccagtagct
    38341 agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt ttattatttt
    38401 cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc aatggataat
    38461 ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg ctgattgatt
    38521 ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa aactgataaa
    38581 agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt tttctttttt
    38641 tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg gatctgtctg
    38701 caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc gattctctcc
    38761 tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa tataaattgc
    38821 cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca
    38881 ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta tctcatatgg
    38941 actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt tcttcagctg
    39001 ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat tcaatccgat
    39061 accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga aatcgatggt
    39121 ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa gggtcaaagg
    39181 tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa aataactgat
    39241 attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag ctgcttcatc
    39301 agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta tacaagaagt
    39361 tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag cgcctttttc
    39421 taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt taaaattcaa
    39481 gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat
    39541 gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat ctgtatccag
    39601 tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt cctgaaacca
    39661 agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg tttcgcttct
    39721 catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat aatttacaac
    39781 ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt
    39841 atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt gctgctcttc
    39901 aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat gcagtttgcc
    39961 tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag aggcggtttt
    40021 ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca tcggattccc
    40081 ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg cttactgtca
    40141 atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa gcttatgtat
    40201 caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag ctattcctaa
    40261 attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat tctaaaacgc
    40321 tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg cgaaaaaagc
    40381 taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata tctctgataa
    40441 agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga gcaaaattgc
    40501 aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc gtacttctca
    40561 tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg ccgtttctga
    40621 acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa
    40681 atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa gctctacgat
    40741 ggatgaaaca cttctttttg aattggatca atataatatt cctattgtgg taatcggaaa
    40801 agtcggaaac gactacacga acatctactc agtggacacg gataatttcc aggatagtta
    40861 cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc tccatgctcc
    40921 gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct tatttgatca
    40981 ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag aagatagcat
    41041 ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt tttctaccga
    41101 tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc agatacctga
    41161 tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt cgccacagtt
    41221 gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac ttttattcca
    41281 actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg agattactct
    41341 cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac agaaacactg
    41401 aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac cccctcccgg
    41461 tattttagcg aatttttctt attttccatc tgttttagat agctgtggat aactataatg
    41521 tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca cgttgtgtat
    41581 aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca tgttatttgg
    41641 ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt ttgttttatt
    41701 cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta tcttcccatc
    41761 aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac ttctcttgga
    41821 agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat cctttcccac
    41881 ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt gacaataatt
    41941 tgtcacaacc tcttttttaa aatatctatt ttgtttgatt gttcattgcg tcttctgcag
    42001 ccatttgcgc ttcaatatct gaaatactgt agacattttc gcttgctaca ccgacttctt
    42061 ttggttccat gtcacgataa cgttgcatac cagtaccagc tgggatgatc ttacctataa
    42121 taacattttc tttcaaacca agaagaggat ctttcttacc gcggatcgca gcatccgtca
    42181 agacacgagt tgtttcttgg aatgatgcag cagataagaa actatttgtt tctaatgatg
    42241 cttttgtgat acctagtaag acaggtcgag aagttgcagg aacgcctcct gagactagcg
    42301 tattgtagtt ttgatcttta aagtctgcga tgtctaataa tgtacctggt aagatatctg
    42361 tatcacctgg atccattaca cgaactttac gcagcatttg acgaaccatt acttcgatat
    42421 gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt
    42481 agttttcaac agataataca tcgcgaactt gcaacaattg ttttggatcg attgatcctt
    42541 ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc tactttcata cgtgctgtat
    42601 aaggaacagt atacgtacgt gtatctgttt ttcctttgat tgttacttct ttggtacgtg
    42661 ttgcaggatc ttctgagata tcgatgacat cccctgttac ttctgtgatc actgcttgcc
    42721 ctttcggatt acgtgcttca aagatttctt ggacacgagg aagaccttga gtgatatcgt
    42781 ctccggcaac cccacctgta tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga
    42841 ttgattgagc ggcgatcgta ccaactgctt ctccgacttc aacgtcagag ccagttgcaa
    42901 ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt attacatgtg aagacagaac
    42961 ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca
    43021 attggtttcc tgcaatgatc aattcatttg tttcaggatg acgtacttct ttacgggtgt
    43081 aacgtcccac taaacgatct tctaatgatt cgatgatttc atttccctcg cggatggctt
    43141 ggatttcaag acctcggtca gtaccgcaat cttcttcacg gatgattaca tcttgggcca
    43201 cgtcaaccag acgacgagtc aagtaacctg aatcggcagt cttcaaggct gtatcggtca
    43261 tccctttacg agccccgtga gttgagataa acatttccaa gacggatagt ccttcacgga
    43321 agttcgaaat gattggcaat tccatgattc gaccattagg agcggccatc aaaccacgca
    43381 ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc tccagaatca gacatcatga
    43441 agattggatt tttcgcttcc aaaccttcca tcagtttttg ttggatgctg tcttttgcag
    43501 cattccaaac agcaatgaca cgttcataac gttcgtcatc tgtgatcaga ccacgacgga
    43561 attgttttgt aatagtttca acttgtttat gggcattttc aatgatttct tgtttttcat
    43621 tcagtacact gatgtcagcg atccctactg tgataccggc atgtgtcgaa tgtttgtacc
    43681 ctaagtcttt catacggtca agcatcttag aagtttctgt tactttgaat cgtttgaaga
    43741 cttctgcgat gatgtttcct aagtttttct tcttgaacgg taatactaat tcttgctctt
    43801 tgatatgtgc aggaatatca gtaccagctt caacaaagta tttgtccggt gtttggacgg
    43861 tcaagttgaa gtctgttggt tcattcagat aagggaattc aggaggcatg atctcgttga
    43921 agatgatctt accaactgtt gtgatcatcg tacgttcttt ttgccattca gtaaatggtt
    43981 tttcacctaa tgagttaggg ttcacaccga tacgtgaatg taaatgaaca tatccgttgc
    44041 gccatgcgat aacggcttcg ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt
    44101 cttcttccat tgtcaagtag tagttaccta gaaccatatc ttgagaagga gtaacaactg
    44161 gtttaccatc tttagggttc aagatgtttt gagcagctaa catcaacata cgtgcttctg
    44221 cttgtgcttc ttcattcaat ggaacgtgga cagccatttg gtctccatcg aaatcggcat
    44281 tataagcttc acatacaagt ggatgcaaac ggattgcgcg tccttgtact aaaactggtt
    44341 cgaatgcttg gataccaagt cggtgaagcg taggtgcccg gttcagcaat actgggtgtt
    44401 ccttgattac gtcttctaaa acatcccata cttcgtcttc ttgacgttcg attttacgtt
    44461 tcgcattttt gatgtttgaa gctagttcgc gttgaacaag ctcacgcatc acgaatggtt
    44521 tgaataattc aatggccatt tcttttggaa gtccacattg gtacatcttc aagaaaggtc
    44581 caacaacgat aacggaacga cctgagtagt cgacacgttt acctagcaag ttttgacgga
    44641 aacgaccttg tttccctttc aacatatgag aaagagattt caatgggcgg ttacctggtc
    44701 cagtaactgg acgtccgcga cgaccgttat cgatcaatgc atctactgct tcttgcagca
    44761 tacgtttttc attttgaacg atgattccag gtgcattcaa gtctaacaaa cgtttcaaac
    44821 ggttattacg gttgattaca cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc
    44881 caccttctaa ttgaaccatt gggcgtaaat ctggtggaat aacagggata acgtccataa
    44941 ccatccagct tggttgattt cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga
    45001 ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc ttctttcaat tcggctgctt
    45061 ctgcatctag atcaacatta tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt
    45121 ggaattcttg tccatattgt tcacgttttt cgcgatattc gcgttctgtc aacaattgtt
    45181 ttttctctaa attcgtattt cctggttcga tgactacata tgaagcaaaa taaatgattt
    45241 cttccaaagc gcgtgggctc atgtctaata caagacccat acgtgaagga atccctttaa
    45301 agtaccagat atgtgaaaca ggagctgcta attcaatatg tcccatgcgt tcacggcgaa
    45361 ctttcgaacg agtaacttcc actccgcatc gatcacaaac gattccttta taacggatac
    45421 gtttgtattt tccgcaggca cattcccagt cttttgtagg accaaaaatc cgctcacaga
    45481 acaacccttc acgttcaggt tttaaggtac gatagttaat cgtctcaggt ttttttactt
    45541 caccgtaaga ccagcttctg attttttcgg gagaagccaa acctatttgc atactttcga
    45601 atttatttac atcgatcaaa aggggttccc tccatttcat ttaatggcaa accgcaagac
    45661 ggctgatctt cgaccgcctt gcagctcata gaccggatta gtctaaatta tcttctgcgg
    45721 tttcaaagtt ttggatgaca tcgtcttttt catcttccac ttgttcagca gctttctttt
    45781 ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat
    45841 cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt ttcttcgata tccaagacac
    45901 gcatgtccag acctagtgat tgcaattctt tcaccaatac gcggaatgat tccggtacac
    45961 ctggttttgg aattggttca cccttgacga ttgcttcgta tgttttcaca cgtccgacta
    46021 cgtcatctga tttgtatgtc aagatttctt gtaaagtata agccgcaccg taagcttcca
    46081 gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg
    46141 gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc atgcaatttg tcatcgacca
    46201 tgtgagcaag tttgatcatg tacatcacac cgacagacac acgtccatca aatggttcac
    46261 ctgtacgtcc gtcgtacaga acagttttcg catcgctggc cataccggct tcacgaactg
    46321 tttcccatac gtcttcatca cttgcaccat caaatactgg cgttgctacg tggataccta
    46381 actgacgtgc agccataccc aagtgtaatt ccaatacttg cccgatgttc atacgtgaag
    46441 gcacccctaa aggattcagc atgatatcga ttggtgttcc atcaggtagg aatggcatat
    46501 cttcttccgg cataatacgg gatacaaccc ctttatttcc gtgacgaccg gccattttat
    46561 ccccttcatg gattttacgt ttttgaacga tataaacacg aactaacatg tttacgcctg
    46621 gtgacaattc atctccagct tcacgagtaa agattttcac atcgtgaacg ataccgccgc
    46681 cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg agctttttcg ccaaagatcg
    46741 catgcaatag acgttcttct gcagataatt ctgttacccc ttttggcgtg actttcccta
    46801 caagcaaatc gccatcttgg acttctgcac caatacggat gatacccatt tcgtctaaat
    46861 cttttaatgc gtcttccccg acattaggga tttcgcgtgt gatttcttca ggtcctaatt
    46921 ttgtatcgcg ggcttctgat tcatattctt caatatggat agaagtgtat acgtcatctt
    46981 ttaccaaacg acggctcatg atgatcgcat cttcatagtt gtacccttcc caagtcatga
    47041 aaccaactaa aacgttttgg cctagagcca tttcgccttg ttccatagat ggtccgtctg
    47101 ccagagtatc ccctttttcg actttctcac ccaaatgaac gattggacgt tggttgtagc
    47161 ttgttccgga gtttgaacga cggaattttg ttacatcgta tttatctaat gcaccgttgt
    47221 cgcggcgtac gcggatttca gaagcatcaa cgtattcaac gacaccgtca tgtttacata
    47281 acaaagcagc tcctgagtca tgcgctgatt tgtattccat accagtacca acccatggtg
    47341 cttgaggatt gatcaacggc actgcttgac gctgcatgtt ggcacccatc aaggcacggt
    47401 tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact acttgtttag
    47461 gtgatacgtc catgtagtct actttatcaa tagatacttc taagttttca ctttgagcac
    47521 gagccataac aacgtcttga gcaaatgttc catcttcatt caatgggctg tttgcttggg
    47581 ctacgatgta atggtcttcg atatcagctg tcaagtagtc gatttggtca gtaacacgac
    47641 ctgtttcacg gtctacacga cgataaggtg tttcgataaa tccgaattta tttactttcg
    47701 cataactgga caaactattg atcaacccga tgtttgggcc ttcaggagtt tcgatcggac
    47761 acatacggcc atagtgggag tagtgaacgt cacgaacttc ataaccggcg cggtcacgag
    47821 tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt
    47881 ttgtttgatc catgaactgt gacaactgag aagaaccaaa gaattctttg atacttgcca
    47941 caactggacg gatattgatc aattgttgag gtgtcaatgt ttcagtatct tggatcgaca
    48001 ttctttcacg aaccacacgt tccatacgtg ctagcccgat acggaattgg ttttgtaaaa
    48061 gttcaccgac tgaacggata cgacgatttc ctaagtggtc gatatcatcg acattgccga
    48121 taccttccat caagttcaag aaatagctca ttgaagcaat gatgtcggct ggacgaaccg
    48181 tttttacagc tgcttctgga tagccgtttc cgatcacgtt tacttcacgt tcagggtctc
    48241 ttggtgaaaa tactttgatg acttggacag tcattggatc tgtgaccaca ccgtcttctg
    48301 atggatagta agtcactgaa tttaggccat tatcaatgaa aggagctagt gtttccatga
    48361 cttggtgagt cagaactgtt cctttttcaa cgatgatctc gcctgtttct ggatcaacta
    48421 atgtttcagc taatgtcaga ttcaatagac gtgttttcaa atctaatttt ttgtttactt
    48481 tgtaacgacc aacgtttgct aagtcgtaac gttttggatc gaagaaacga gcattcagca
    48541 agttacgtga gctgtctgct gtttttggtt cgcctggacg caaacgttcg taaacgtctt
    48601 tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg caagtctttt tcgactgtat
    48661 tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt atcatcggaa ccaaatccta
    48721 acgcacgaac taatacggtt aaaggaatct tacgtgtacg gtcgatacgt acataagaga
    48781 tgtcttttgc atcagtttcc atttctagcc aagctccacg gttagggata acagtagaac
    48841 cgaatccttc ttttccgttt ttgtcgactt ttccatggaa atagacacct ggtgaacgga
    48901 ctaactgaga aacgattact cgctctgccc cgttgatgat gaatgttcct tgttctgtca
    48961 ttaatgggaa atcaccgaag aatacttctt gtgctttgat ttcgccagtc tcgcggtttg
    49021 tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc atcatgcgca cgagcttctg
    49081 ctactgtata ctttggttct ttcaattcat aatctacaaa ttccaaagat agattgccat
    49141 tgaagtcatc gattggcaaa atgtcttcaa acatttctct aagtccctca tctaagaacc
    49201 attggtaaga gtctgtttga atttcgatca aatttggtaa ttccaacact tcactgattc
    49261 ttgcgaaact tctacgttcg cgatgttttc cgtattttac tacgtgtcca gccaagctct
    49321 tcacccctct aaaatgtcat tcaaaaagac tacccgatca attgttacaa taatatttca
    49381 aatattattt ttaggtaaca acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa
    49441 aatagaacat tctcttgaaa ccttacttcg tgagcttcta cttttgtttc cttatattaa
    49501 agccgaaacg gacaatattt cgtttctgcc ttttcttcat cagatagttt ttgcaacttc
    49561 atatactaca cttattttct ataaaagtca acacttcctg gcgtttcttc tgctttttcg
    49621 caacattttc ttaatgtttt caattttatg ttttcaatta tctgacaatt cttaattgac
    49681 agaccttttt atctcggtta tactttagaa aattcatttt gtggaggaac ttctatgcga
    49741 accaacatca accaaatcgc accaacacaa ctgaataact tttactttag ctactttaga
    49801 taggtttgta ttcatcatga tggatacaaa cgttaaacga cagtttgtat ctatgatggc
    49861 taaagaactt tgtattgcag caaaaagcca cgtagacaaa atgctaagtg gctgagcagt
    49921 gaaaatacaa agtaaaagta tcaagcattc tactttttca atttattttc cgacttttca
    49981 atccatttgg acattttgtc tgaatggatt ttttgtttcc caaaattaag gaggaaaaag
    50041 aaatgaaaaa aaatataatc atcggattag gcgctgccct gctactagct ttaggaggtt
    50101 gcgcacaaaa agacaattcc gcagaggcat cagatggaca aaaaacaatt ggcgtattgc
    50161 aacttgtaca acataactcg ttagatgctg cctataaagg attcaaagaa ggattagcag
    50221 aagaagggta taaagaaggc gataatctaa caatcgatta tcaaaatgcc caaaacaatc
    50281 aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga agatcctgat ttgctacttg
    50341 gaatcgccac tccagcagca caatctttat taaatgaaac aaccactatc ccgatgactg
    50401 tcacagcggt cacagacttg gaacaagccc atttagtcac atccaatgaa aaacctggcg
    50461 gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa acaaatcggc ttactgttaa
    50521 atatcgtgcc agacgccaaa acaatcggca tcatgtataa caacggtgaa gcaaactcta
    50581 agatccaagc tgatttggca gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac
    50641 tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa aagtctagct aaagatgtag
    50701 atggaatcta tattcctact gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag
    50761 tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg
    50821 gtctagcaac agtaggaatc gactacgaaa gcttgggcaa acaaacaggg atcatggcag
    50881 caaaaatctt agacggcaag aatccaagtg aacttcctgt cgaaacagcg aatgatttgg
    50941 aactgtatgt caataaagac atggccaaag cattaggcat caatccagac agcatcaaag
    51001 aagctgaata aaacacagga ggattctcat gacaaatttg ctctctcttt tcttatcatc
    51061 tgcatcgcaa ggggtcttat gggccctact tgcgattggt gtatacgtca ctttccgtat
    51121 cttggacatt gccgacttga ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc
    51181 agtaagtatt acaaatggct atcatccatt gacagcttgt cttctaggct ttttaggggg
    51241 tgcggcagca gggcttgttt ccggactgct tcacacaaaa ttgaaaattc ccgcccttct
    51301 tgcagggatc atcaccatga ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc
    51361 gaacatctct ttattgggtc agccaacgat ctttcattgg gtcgagtcat tcggcataac
    51421 aaaagacaac gctgtactga tcgtcggtct gctttttgct ttgatcgttg tatgtttact
    51481 tgttttgttc ttacgtacag aaacaggact tgctattcgt gcaactggcg ataatctggc
    51541 aatgagcgaa gcaaacggga tcaacacaga taatatgaag atcatcggtt atatgatttc
    51601 taatggatgc attgctcttt ctggctcctt acttgcgcaa aacaacggct ttgctgattt
    51661 aaattctggt attggaacga tcgtaattgg acttgcttct attatcatcg cagaagtttt
    51721 gttacgtaac cagccactat ttctccgtct cttgactgtc gttttaggtg cggtcattta
    51781 tcgttttatt ttagcgttag tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc
    51841 ttctgctttc gtgctagtga tctgtctttc tttcccgcag atccagcaaa aattccattt
    51901 acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc
    51961 catcatacct ttgaaaaagg aacgatcaac gaaaatcatg tcctgaaagg cattaatctt
    52021 tccttagatc ccggcgactt cgtgacgatc atcggtggaa atggggctgg taaatctact
    52081 ttgctgaaca gcatcgccgg tacattttct gtcgatcagg gtcaaatttt attgaatgga
    52141 aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa
    52201 gatcctaaac tagggacagc cgttcgttta acggtagaag aaaatcttgc tcttgctatg
    52261 aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac cacaagaccg aacatttttc
    52321 aaagaccagc tggttcggct aaatttgggg ttggaaaatc gtttatcctc agaaatcggt
    52381 ttgctgtctg gaggacaacg tcaagctatt acgttattga tggccacatt acagcgtcca
    52441 gaactgatct tattagatga acacactgca gcgcttgacc ctgctacttc caaaacggtg
    52501 atggaattga cagaaaagct gatcactgag cagaatctca ctgcttttat ggtcactcat
    52561 gacatggaag acgcgatccg ctatgggagt cgactgatca tgctgcatca aggaaggatc
    52621 gtcgtagata tccagcaaga ggaaaaacag cagctcacag tgccccaatt attagcgttg
    52681 ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag tacttttatc ttcataatca
    52741 gaaagaggat gggacaaaaa atgaacgccc accctcttct tttttctctt tccttctttc
    52801 gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact attttgttct ctccttcttc
    52861 ctgatagaat ggatgggaaa ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa
    52921 ttattgaaaa agacaccaga ttttctttcc ggagaaaagt tggcacagca actggaagtt
    52981 tcccggacaa gtatttggaa agccatcaaa gaattggaaa aggcaggcta tcgttttgag
    53041 cacgggccta cagggtatcg ttacctgccc tctgacgtac tagatgcaaa agaaatccag
    53101 caagagctac ggtcgtttat tcctgatttg aaagtacaaa cgatgttgac ttctgaatct
    53161 acgatgaaag atgccaaact ggcagcagct caaggagatt ctgtttcgaa gttatttgtt
    53221 gctgatacac aagaacaagc tcgtggacgt tttggacggc cttttttcgc tgaacctgga
    53281 cgaggaatct acatgagtct cttgctccat ccagacaaac ggtttgatga attgcctcaa
    53341 tatacgatca tcgcagccag tgcctgtgtg aaagccattg aatctgtaac cggtcgttct
    53401 gcttcgatca aatgggtcaa tgatatctac atggatggaa aaaaagtctg tgggatttta
    53461 tctgaagcag tcagtgatat ggaaagcggc aggatcagtc atatcatcat tgggatcggg
    53521 cttaattttt ctgttcctca aaatcatttt ccagctgatc tccaagaaaa agccatctct
    53581 gtctttcccg acggacaagc tagtgtcaca aggaatcagc taatcagcaa aatctggcaa
    53641 gaattgttca cttatctttc tcacccagaa aaagcgttgg acttctataa agaaaaatca
    53701 ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca
    53761 aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa atgaacagaa gatgagtttg
    53821 tcttctggcg aaatcagtct cacatctatc cgtacgcaat aattatcaga tttcacaaaa
    53881 ggacacctga tctcatacag tgtccttttt tctgaattct cttcaaaaga ctctccttat
    53941 tctatcagct gacagtttct ctatcctcta gaaaaaggta ttctaaaaat atctataaag
    54001 catggtacac tttctttaaa tgaatcaatg caaaaaacaa gagagtcatc agtttacacg
    54061 atcaaaagag atagcaggcc gcttcctttt tcaatcttat agatagacca tgggtaacga
    54121 taaaaacaaa aaagaacttg cgacttattt tgccgtaagt tcttttttag ctagtgcctt
    54181 aatctcttaa tcccaacaaa cgtctcgcat agatcacctg accagagtgc atagcagcat
    54241 catcaataat agaaacgaga cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa
    54301 ttacttcatc tagctcggaa ggttccagcc ttcttaagta agaaatagca aaatcagcag
    54361 ctcctcgcaa gtatcctagc agatcttcta tttgattagc ttggatctgc tttgcttgtt
    54421 ccagactatg cttccaatct tgttcgttat ctgctatcgt catcggaaac cggctttccc
    54481 agtcttgact atgccaaatt ggagaagttc ctgctaaatc agcaatctgg aaatctaatt
    54541 ctcgagcggt atgccaagcc agccaagtca ttgatttgat ctgcggggct ctatcttcca
    54601 ctggaaaacg attggcctcc tcaatagaga cgccttcaaa caaacgttca aaccgatcgt
    54661 atgcccgatc cacactctcg catagcatat ctaaataatc catttatttt cctccttttt
    54721 atttatccta cttacttttt cctaattcta ggatacctac ttatatgtta ctacacaccc
    54781 ttatttttta cactttaaca gtcaccatca tcaatagttt ggagattgaa agaaaaagat
    54841 agtgtcgaac tatcaattga acaagaagca acaatatgaa cgtttcatta atattcttta
    54901 aataaacaat gatttttcaa acgaacagtt taagactagg agaaaaagag tataatagga
    54961 aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag
    55021 ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa acatacattc
    55081 ctaaagaccc tgggaatatt tataatgatc taactggtaa ctatggcttg ttaggaattg
    55141 ctagtcaatt ccacgtattt gctaaaaata agacaactgt gaatgcacat acagacggaa
    55201 atatagcaac acatgaactt gatgcaaaaa ataattttgg tacagagctt catgaaggtt
    55261 tattgaacca agaaataaat tatatacaaa aaactgattc tttcgctaat tcttcaggca
    55321 tacctacagg tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac
    55381 ttcaagatgg tcatcctaca gtcaatggat ccaggcttga tcacttaact ccagatgatt
    55441 tttataaaga tcgtgctggg catgaatata ttgatttcga gcaggaattt gctaaattga
    55501 atgtagcagc aaatgatctt gcaacgatca ctccagctaa aacatatact gctgcagatt
    55561 ttccagacat gaacaatcgg acaatcgatt taactgattt atcagatacc gggtttttat
    55621 tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc attaatccaa
    55681 atgatcaagt agttgtattt aatgtcatca acagttcttc tgcattgaac gtacagtcac
    55741 caatcaaata caatgatcgt tcaaatcatg aaacagaaga tttttcggat gcgaatatct
    55801 cttggaattt tggaaatgag atgactgatt tgacaatcag tgctcctttt caaggaacaa
    55861 tccttgcgcc taatgctact atccgcatta ctcaaaacca agacggaacg attataggaa
    55921 caaatgtgat tttagatgca gctaccaatc gttgggatcc taacgaaata tttatcagcg
    55981 acaatggtac agatacgaca gacacaagcg acagtacaga tacatctacc acaagtgata
    56041 gctcagattc atca
//
LOCUS       DAZKAS010000003        54428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000003 DAZKAS010000000
VERSION     DAZKAS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.003"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..750
                     /locus_tag="ACG8H7_000115"
     CDS             <1..750
                     /locus_tag="ACG8H7_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982258.1"
                     /translation="VFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLD
                     LGISSFGKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTM
                     GQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIY
                     LSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKEL
                     MKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(990..1073)
                     /locus_tag="ACG8H7_000116"
     tRNA            complement(990..1073)
                     /locus_tag="ACG8H7_000116"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1037..1039),aa:Leu,seq:gag)
     gene            1146..1307
                     /locus_tag="ACG8H7_000117"
     CDS             1146..1307
                     /locus_tag="ACG8H7_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982259.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            1456..2673
                     /locus_tag="ACG8H7_000118"
     CDS             1456..2673
                     /locus_tag="ACG8H7_000118"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP8982260.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2857..3693
                     /locus_tag="ACG8H7_000119"
     CDS             2857..3693
                     /locus_tag="ACG8H7_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982261.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3769..4917
                     /gene="nagA"
                     /locus_tag="ACG8H7_000120"
     CDS             3769..4917
                     /gene="nagA"
                     /locus_tag="ACG8H7_000120"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP8982262.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5194..6195
                     /locus_tag="ACG8H7_000121"
     CDS             5194..6195
                     /locus_tag="ACG8H7_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP8982263.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            6214..7233
                     /locus_tag="ACG8H7_000122"
     CDS             6214..7233
                     /locus_tag="ACG8H7_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFP8982264.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7335..8051)
                     /locus_tag="ACG8H7_000123"
     CDS             complement(7335..8051)
                     /locus_tag="ACG8H7_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8982265.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(8020..8805)
                     /locus_tag="ACG8H7_000124"
     CDS             complement(8020..8805)
                     /locus_tag="ACG8H7_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8982266.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8796..9545)
                     /locus_tag="ACG8H7_000125"
     CDS             complement(8796..9545)
                     /locus_tag="ACG8H7_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP8982267.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9843..10367
                     /locus_tag="ACG8H7_000126"
     CDS             9843..10367
                     /locus_tag="ACG8H7_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP8982268.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10383..11882
                     /locus_tag="ACG8H7_000127"
     CDS             10383..11882
                     /locus_tag="ACG8H7_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP8982269.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11970..12572)
                     /locus_tag="ACG8H7_000128"
     CDS             complement(11970..12572)
                     /locus_tag="ACG8H7_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP8982270.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12676..13632
                     /locus_tag="ACG8H7_000129"
     CDS             12676..13632
                     /locus_tag="ACG8H7_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFP8982271.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13937..14893)
                     /locus_tag="ACG8H7_000130"
     CDS             complement(13937..14893)
                     /locus_tag="ACG8H7_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8982272.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15067..15627
                     /locus_tag="ACG8H7_000131"
     CDS             15067..15627
                     /locus_tag="ACG8H7_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982273.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15620..16444
                     /locus_tag="ACG8H7_000132"
     CDS             15620..16444
                     /locus_tag="ACG8H7_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFP8982274.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16454..17071
                     /locus_tag="ACG8H7_000133"
     CDS             16454..17071
                     /locus_tag="ACG8H7_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP8982275.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17089..17772
                     /locus_tag="ACG8H7_000134"
     CDS             17089..17772
                     /locus_tag="ACG8H7_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP8982276.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18455..19690
                     /locus_tag="ACG8H7_000135"
                     /pseudo
     CDS             18455..19690
                     /locus_tag="ACG8H7_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            19698..20585
                     /locus_tag="ACG8H7_000136"
     CDS             19698..20585
                     /locus_tag="ACG8H7_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982277.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20602..21438
                     /locus_tag="ACG8H7_000137"
     CDS             20602..21438
                     /locus_tag="ACG8H7_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982278.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21441..22064
                     /locus_tag="ACG8H7_000138"
     CDS             21441..22064
                     /locus_tag="ACG8H7_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982279.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22061..23353
                     /locus_tag="ACG8H7_000139"
     CDS             22061..23353
                     /locus_tag="ACG8H7_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8982280.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23712..23809
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23925..24506
                     /locus_tag="ACG8H7_000140"
     CDS             23925..24506
                     /locus_tag="ACG8H7_000140"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP8982281.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24509..25816
                     /locus_tag="ACG8H7_000141"
     CDS             24509..25816
                     /locus_tag="ACG8H7_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFP8982282.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26109..26597
                     /gene="purE"
                     /locus_tag="ACG8H7_000142"
     CDS             26109..26597
                     /gene="purE"
                     /locus_tag="ACG8H7_000142"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP8982283.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26590..27714
                     /gene="purK"
                     /locus_tag="ACG8H7_000143"
     CDS             26590..27714
                     /gene="purK"
                     /locus_tag="ACG8H7_000143"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP8982284.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27727..29022
                     /gene="purB"
                     /locus_tag="ACG8H7_000144"
     CDS             27727..29022
                     /gene="purB"
                     /locus_tag="ACG8H7_000144"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP8982285.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29202..29579)
                     /locus_tag="ACG8H7_000145"
     CDS             complement(29202..29579)
                     /locus_tag="ACG8H7_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982286.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29835..30563
                     /gene="purC"
                     /locus_tag="ACG8H7_000146"
     CDS             29835..30563
                     /gene="purC"
                     /locus_tag="ACG8H7_000146"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP8982287.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30563..30817
                     /gene="purS"
                     /locus_tag="ACG8H7_000147"
     CDS             30563..30817
                     /gene="purS"
                     /locus_tag="ACG8H7_000147"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFP8982288.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30819..31490
                     /gene="purQ"
                     /locus_tag="ACG8H7_000148"
     CDS             30819..31490
                     /gene="purQ"
                     /locus_tag="ACG8H7_000148"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFP8982289.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31491..33713
                     /gene="purL"
                     /locus_tag="ACG8H7_000149"
     CDS             31491..33713
                     /gene="purL"
                     /locus_tag="ACG8H7_000149"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFP8982290.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33698..35137
                     /gene="purF"
                     /locus_tag="ACG8H7_000150"
     CDS             33698..35137
                     /gene="purF"
                     /locus_tag="ACG8H7_000150"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP8982291.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35160..36212
                     /gene="purM"
                     /locus_tag="ACG8H7_000151"
     CDS             35160..36212
                     /gene="purM"
                     /locus_tag="ACG8H7_000151"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP8982292.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36209..36787
                     /gene="purN"
                     /locus_tag="ACG8H7_000152"
     CDS             36209..36787
                     /gene="purN"
                     /locus_tag="ACG8H7_000152"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP8982293.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36784..38319
                     /gene="purH"
                     /locus_tag="ACG8H7_000153"
     CDS             36784..38319
                     /gene="purH"
                     /locus_tag="ACG8H7_000153"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP8982294.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38335..39609
                     /gene="purD"
                     /locus_tag="ACG8H7_000154"
     CDS             38335..39609
                     /gene="purD"
                     /locus_tag="ACG8H7_000154"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP8982295.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39731..40645
                     /locus_tag="ACG8H7_000155"
     CDS             39731..40645
                     /locus_tag="ACG8H7_000155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP8982296.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40778..42343)
                     /locus_tag="ACG8H7_000156"
     CDS             complement(40778..42343)
                     /locus_tag="ACG8H7_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982297.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42336..43787)
                     /locus_tag="ACG8H7_000157"
     CDS             complement(42336..43787)
                     /locus_tag="ACG8H7_000157"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8982298.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43801..44817)
                     /locus_tag="ACG8H7_000158"
     CDS             complement(43801..44817)
                     /locus_tag="ACG8H7_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8982299.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45037..46125
                     /gene="chvE"
                     /locus_tag="ACG8H7_000159"
     CDS             45037..46125
                     /gene="chvE"
                     /locus_tag="ACG8H7_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP8982300.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46179..47705
                     /gene="mmsA"
                     /locus_tag="ACG8H7_000160"
     CDS             46179..47705
                     /gene="mmsA"
                     /locus_tag="ACG8H7_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8982301.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47707..48912
                     /gene="mmsB"
                     /locus_tag="ACG8H7_000161"
     CDS             47707..48912
                     /gene="mmsB"
                     /locus_tag="ACG8H7_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFP8982302.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49169..50146
                     /gene="guaC"
                     /locus_tag="ACG8H7_000162"
     CDS             49169..50146
                     /gene="guaC"
                     /locus_tag="ACG8H7_000162"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP8982303.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50410..51834
                     /locus_tag="ACG8H7_000163"
     CDS             50410..51834
                     /locus_tag="ACG8H7_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP8982304.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(51876..52133)
                     /locus_tag="ACG8H7_000164"
     CDS             complement(51876..52133)
                     /locus_tag="ACG8H7_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982305.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            52295..53404
                     /gene="pdhA"
                     /locus_tag="ACG8H7_000165"
     CDS             52295..53404
                     /gene="pdhA"
                     /locus_tag="ACG8H7_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFP8982306.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53407..54384
                     /locus_tag="ACG8H7_000166"
     CDS             53407..54384
                     /locus_tag="ACG8H7_000166"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFP8982307.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17401 a         9313 c        11898 g        15816 t
ORIGIN      
        1 gtattttcaa taataagctt agcagaatta attttctctt tgacaactcc agggaatgca
       61 ttacgtgctg cacatgaaat taataaatgg tttcctgaat ataaaaattt caactttctt
      121 aataagttag atttaggtat ttcatcattt ggaaagccgt tttttttaga catgaatatc
      181 ttatttttat tgctattttt cttaattttc cttctaacat ataggaaatg tcaaaactat
      241 tatgtgcgta tattgactgc attaccgttt ttcttaaacc tgattattta ttttggtaat
      301 acaatgggtc aaagctttac ttacgtcaat ggaaataaaa gggcaatgat ttggagtagt
      361 agtaacctga ataatctctt tacagagtta ggaacgaaac tgtcactttt ttatccaggt
      421 acttggatag ctactttagt agtcttggca ttattactat gtttaattgt tggaatttat
      481 ttaagttttg acaataagaa aacttctatt tttttagtta ttttgatgat aatgggattt
      541 tgttcaaggt taattatggg attttctccc acagtatggg cttccggaat gcggacgtac
      601 tatatacttt atgttgttat cgccatctta gtactaatgg cagttaaaga gctaatgaaa
      661 agcatgagtg tacaaaaaaa tgaatttatg caatttggat taaccgtgct tggtatttgt
      721 acctttataa taacggtgat aaacagataa tatacaaaaa atccctcgct ttagtattag
      781 gtgagggatt ttactcgttt ttcgattaat tgttttaaca cttgcaagtt tctacgagta
      841 gcttgtttcc gaccttcata tactcacctc aggctgttta catgattgta cgttcttttt
      901 gtaaaggata cagtgatatg tatataatgg cataaagaaa cgttcaaaca aaaaaactcg
      961 tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg ggactcgaac ccacacgagc
     1021 taacgctcac atgaccctca atcatgcttg tctgccaatt ccaacacatc tgcgtaataa
     1081 taacacacta atgatagcga ggttttacat agttgtcaag ctcaaaaatg ggaggactta
     1141 ttaagatgga gaaatatgac ggagaatttt caggactggg aatgatctta ggtgtattga
     1201 ttggacttgc attcggacgt tttttgcttg gttttatgct aggaatcatt tgtggaattg
     1261 caatggattg ggcagcgaat ctatggaatg actatcatga caattaagtt ctcaacgctt
     1321 gtttgtgttt ttttaatact tacgtttttc ctaaataagt ggcaaaattt tctaggatgg
     1381 agtatgatat tgaaatagta aataacaaaa aatttggaaa gttctaactt tttgaaatga
     1441 gggattggct aagtgatgag agaatttgaa aaatccaaca aattagaagg tgtcagttac
     1501 gatgttcgcg gtcccgttct agaagaagcg gagcggatgc aagaagaggg cattagtatt
     1561 ttaaaattga acacgggaaa tcccgcacct tttggatttg aagcaccaaa cgaagtcatt
     1621 cgtgatttga tcatgaatgc ccgtgactca gaaggctact cagattccaa ggggatcttt
     1681 tctgctagaa aagcaattga gcaatattgc caactgaaac attttccgaa tgtgacgatc
     1741 aatgatatct acaccggcaa cggagtgagc gaattgatca cgatgtgtat gcaggggttg
     1801 ctggacaatg gagatgaagt actggttcca atgccggact atccgttgtg gacggcttct
     1861 attgcattag ctggaggcac acctgttcat tatatttgcg atgaagaggc agaatggtat
     1921 cctgatattg atgatatcaa atcaaaaata acgagtcgga caaaagctat tgttatcatt
     1981 aatcctaata atcctactgg ggcactttat ccaaaagaat tattagaaga aatcgttgaa
     2041 gtagctcgac aaaataacct gattatttat tccgacgaaa tctatgatcg cttagtgatg
     2101 gatggtctag aacatattcc gattgctaca ttagcgcctg atttgttcgt tgttacgctg
     2161 aacggtctat ccaaatctca tcgtgtagca ggatttcgag taggctggat ggtcctaagt
     2221 ggagataagt cacacgtcaa aggctatata gaagggctga atatgttgtc ctccatgcgt
     2281 ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg cgctaggcgg ttatcaaagt
     2341 gtcgatgagc tgctcctgcc aggcggaagg atctatgaac aaagagaata tatctataat
     2401 gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa aagctgcttt ttatatattc
     2461 ccaaaaattg atacaaagcg attcaatatt ttggatgatg aaaaattcgt tttggacttt
     2521 ttacatgaac atcatattct acttgtacat ggtggtggat ttaattggaa ccagccagat
     2581 cattttcgaa tcgtttatct tcctaaaatg gatgatttga aacaaacagc taagaaaatg
     2641 agagaattcc tagcaaccta tcgccaaaaa tgattttaaa aaccaaattt ttcagtagga
     2701 gggccacaga caaacacact gaggctctct ttctagtgga gttttcttaa ataactaaag
     2761 tatattattt agttgcaagt cttttcttga agtttgcgac ttcctaaagt aaaatacgaa
     2821 tagaatgcga atacgcatat tttggggagg atacttatgg aaacgttaat cgagtccttt
     2881 ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga cgcaaaaaat gctttcgcat
     2941 gatatttgtt cacaaagtgt attaagtcgg atcgaaaata atgaggaact gccgaatgta
     3001 ttggtgatga tgcagatttg tcaacggcta ggggtaacga ttgatcatgt gatgaatctc
     3061 tctcgaaaaa ctactcggac aaacgataaa ttatttgaac tgttggatag ttgtttccaa
     3121 aaacgtgatt atcggaagtt agaacaagta ctaaaatctt ctgatatttc tgaaaatctg
     3181 tatcaagcaa ctgactttca acgctattat tattatctcg gtgtttgcga attcacgttg
     3241 cgtcaaaata tctctcagtc cctttattat ttgaaagaag cattaagcta ctctagccaa
     3301 aaagaaagaa tccatgtttc tgacacagaa atacaactga ttagttgtat cggaaaaata
     3361 tatggtgtgg caggaaaaac ggctgaagca cgctactatt tgagtcgaag catccaactg
     3421 ctgcatcaat cagctgatga acgatcaaaa agtgaatttt cgcaaatttt ttataattat
     3481 ggaaatttct tattccatca aaatgaaatc gacgaagcgt tagaacaggt gaatcaagga
     3541 atctattggg ctcaagagaa gaatagctac tattacctca atgatttatt tgtgcttaaa
     3601 agcttaatat acaagcgtaa agaagaacca gaaagagcag cattctatga aggcttagca
     3661 caagctgtga aacaaatttc aaaaaatttg taaatggtgt agaccattct tgacttcttc
     3721 tctaaataag agtactttag aggtagaaaa acaaagggag agaatagaat gagaactttt
     3781 atctatgctg acaaattctt tttagcgtca ggtgtcaaac atgcaggcta tcttgaaatt
     3841 accgatggac tttttggtga atacaccaaa gaactacctg agggagaagt caacatcatt
     3901 gaccaaagcg gcaaatggat tgctcctggt ttggtagata cacatattca cggctatatg
     3961 aatcatgatg taatggataa tgatgcagaa ggcatcaaag tgatgtctga aggtctgtta
     4021 tcttgtggag tgacttcatt tttaccaaca acattgacgt caagcaaaga acgtttaaaa
     4081 gatgtagctg agacaatcgg caaaatgtat caggaagcac caggagctaa aatcaaagga
     4141 atctattttg aaggtccttt cttcacagaa gaacacaaag gggcacaaaa cccaagttat
     4201 ttcggggatc ctgatttgga tactttccat gaatggcaag aagcttcagg tggtttaatc
     4261 aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag agtttgtcaa acaagtgact
     4321 gaagaaggag tagttgtttc tctaggacac agtaatgcta cactagaaga agcgcaaaaa
     4381 gctgttgaag caggcgcaag tgtatttgtc catgctttca atggtatgcg tggattgaat
     4441 catcgcgaac caggtatggt gggtgcgtta ttatctttag aacatgtctt ttctgaattg
     4501 atttgtgatg gccatcatgt acatccaaat gctgcgggaa tcctaatgga aaaagcagga
     4561 catgatcatg tcgcattaat tacagactgt atgatggccg gtgggatgcc tgatggaaat
     4621 tacaatttag gcgaatttcc agtagtagta aaagaaggaa cagcacgtct agaatctggc
     4681 aacttagctg gaagtatatt gaaattgaaa gaagcaatta aaaatgttgt agactgggga
     4741 ttagcaacac cagaacaagc agtcatgatg gctagtctga ttccagcaat cagctgtaaa
     4801 atcgatgata catgtggaaa aatcgctcaa ggacgcgatg cagactttat cgtattgaac
     4861 cctgatatga cattggaagc aacttatcta gatggcgtgg aacgtttcca cgcataatat
     4921 aatttaagtt aaccttaaaa ctaaacttct taagtttggt tttaaggttt ttttgtgttc
     4981 tagtaaaaat cactttacat aagtttcaaa agtttcttta atttcctatt gttcaataag
     5041 taacaatgta aagataaaat taaaaatgtt aatacgtatt agcaaaataa aagctttttt
     5101 tattgtgaat aaatgaacat aattaggagg agaaagatga acttatttga catttttcga
     5161 tcgactttga cagatatgtc gattgtcagt gcaatcactt caacagtttt tattatatta
     5221 ttagggtttg tttgtagaaa aaaggggatt ttttctgaat ctgttggaaa aattctatcg
     5281 aaagtcgtct taacagtggc tttgccagca ctggcatttc gagctttcat gcaagatatc
     5341 aatacagaat tactgatgca aggaatcaat gtcttgattt ggggattagt tatttatatt
     5401 attcttattc tagtttcaaa accttttttc ttaagctata aaggggataa gcaaacaacg
     5461 ttgcgtgtcc tgacgatctt tggttctacg acctttttcg gaacgcctat cgtcagtgcg
     5521 atttatggtc ctgtcggcgt gatgtattcc tcaattttca atattggcta ccgaatcttt
     5581 ttgtattctt atggatacat aaaaatgagt ggcttgaaga tggaagctaa aaatatcaaa
     5641 gcgatgttcg tcaatccaat cattatcgct acttttgccg gccttttcat ttggctgttc
     5701 caagggtatc tgccacaagt gtcagttaca ggtgcagaag gagagataag ccaagtagcg
     5761 tttttgcgaa ttgaccagac tgcaccgtgg ctatttcagc cgatggatta tttagctggt
     5821 ttaagttcac cattggcgtg gctagcaatc ggcgcaacat taggtgaagt aagcttcaaa
     5881 aaagctgcat ctgaaaaaac ttcatggtat tacagtctag tcaaagtggt atttgtacct
     5941 gcattgaata tcgtgattct agcagttttg acggcaacga atattctacc agtcaatttt
     6001 gaagcactgg caacaatcgt tatcatgatg gctacaccag ctgcaacagt ggcagcagcc
     6061 tatgctatca gttttgatag ggaggcactt ttagcctcca atgcatcctt actgtcaact
     6121 atttttgctg tggtactgac acctgtatgg atcgttgtat tacaaatgat tcatggcata
     6181 ggtttatttt cataagaaat ggaggaagaa agtatgagaa tcgcatgtta cggtgttcgt
     6241 ccgaacgaga tagatttttt tgaaaaattg aatatttacc attatgatct cagtctttat
     6301 gaagaactgt tgacacatga caatatcgtg tcagcaaagg accatgatgc tgtattgttg
     6361 aggggaaatt gtatagcaga tgagaccaat cttgcaaaaa tgcaggaata tgggattcgt
     6421 tatgttttta cacggactgt gggagtcaat catatcgatc tacaagcggc agctgatttt
     6481 ggtatgacag tagcccgtgt accatcgtat tcgccaaatg cgatagcaga gttatctttg
     6541 acttttgcca tgatgctttt gagaaatact gcgtatacga cgatccgaac atctttcaaa
     6601 gattttcgag tggatgagca aatgtttagt cgtgaaatca gaaattgcac agtagggatc
     6661 ataggaacag gcaggatagg gttgacggaa gctgttttgt ttaagggact aggagccaaa
     6721 gtgatcggct acgatattca tcagacagaa gctgctaaaa aagtattgac gttccactcg
     6781 cttgaagagt tattagccaa aagcgatatt gtcagtattc atgtacctca tatcccaggt
     6841 gaaaacgatc aaatgatcaa tgaaggattt ttagctcaaa tgaaaaaagg tagcatcttg
     6901 attaatacag cccgtggaga gcttcaagat aatgaagcta ttttaagagc tttgaaaagc
     6961 aatcatttag ctggttttgc aaccgatgtt tttgcaaatg aaacagaggt ttttttccga
     7021 tccttcaagc catgggagac gattccagac cctgctatcc agcaattagt agaaatgtac
     7081 cctagagttc ttgtaacccc gcatgtaggt tcaaatacag atgaggcatt atcaaatatg
     7141 atttcgacaa gtttcgagaa ctttcgtgaa ataatagaaa ctggaaaaac gaaaaatgag
     7201 gtatctcttc ctaaagcccg tcaattaaga taagaaaaca tggatattcg tcaataaaat
     7261 gtttaaaaat agtccaagta aacagttgtt acttctgtat acttggacta ttttgtatgg
     7321 gataattcga tgactcaaca gtaatctgtg agctttttcc gcccattctt tgtcaattca
     7381 taacaggtta gcggctggcc gatttccaaa tatttgattc tttcgttcaa tatatcttgt
     7441 tctactaaaa aattcaaata tttcttgacg gatatacgtg acagtgagct tttttgagcc
     7501 agttcttttg ttgaaaaaat agtatcttct tttagaataa atgcacttat tttttttaaa
     7561 gtcagttttg acagaccttt aggcaaatcg atgaatgatg gtatttcgtg aatgggagtg
     7621 gtgtggttga atacttgatc gatcaatttc tgatcggctt tttcagtcgt ttggattgtc
     7681 tgagagaaag caagatactt ttcgatcgcc agctgaaaac gttgcaaagt gaacggtttg
     7741 atcaaataat ctaaggcacc gtagcggatc acattcttta tggtttcgat atcatctgca
     7801 gcagtgatca aaatcactgg gatatcattt ccttgctgtc taagccaaga taaaaaatgg
     7861 ataccacttt cgccaggcat gtatacatca agcagcagca gatctgcgtt tttttgtctg
     7921 aaatattttt ttccttcttc tacatttctg aaactttcga ttacgttcat attagggatg
     7981 ctttccacaa aagtacgatt caattctgca accattggat catcttcgat tatcaggaca
     8041 tttgtcatca ttttgatctt ccttttttgg taattctaga tagacgattg taccgccatt
     8101 tgtttgtttg gaaaattcta aaaatccacc gtggcgtttg acgattgaat ggatgagatt
     8161 caatccaaac ccacgattga ttcccttcgt cgaaaaacct agcgtaaata ttttttctga
     8221 gatttcttct gatatcccga caccgccatc gcagacggtc acgatgaata cttcagcttc
     8281 tgtgtcatag tttaaatgaa tccggacctg tccgtttctt tcatgttttt gtagcacggc
     8341 ttcttttgca ttatcaagta agttccctaa cacttgcaaa agtgaatgga tggagtcatc
     8401 catctccatt ttagggataa aagaatcgct ttctaacagc atttcaacag attgttcttt
     8461 ggcttcgttt attttgccta ataaaaaacc ggcaattgca ggtactttga tcagacttga
     8521 gacttggcca atctcttttt tataattttg atttagctgc tggataaaat gggtgacttc
     8581 ttcatacttc ttcaaatcaa tcaaaccaga aatgacatga agtttattca tgaattcatg
     8641 ggtttgagct ctcatggagt ttatatattg tttcgtcccc ccaagctcct ccattaattt
     8701 cattacttct gatcgatttc gtaaagttgc gactgctcct aaaaaacggt cattgctaaa
     8761 aagaggtgta acgctagaaa tagtttctat cccatttaaa tacatggatt gagtccgaac
     8821 ggtcgtttga tgctcaatcg tctctttaaa caaaacgtca tatagattat cactgatttt
     8881 tttcctactt cgaaatccaa tagctgctct gtttggtaga aaatgttttt tgcaggtgta
     8941 ttgatcaaga ggatagtttt atcagaagag atggctaaga ttccttcatc tacttcattt
     9001 tcgataatcg ttttttcact taactgttct gctatttctt gtggttcaag tccaagcagt
     9061 acttttttca ttttttgggc aagaaaaacg gcgcctatga tgcctacgct aagtccaata
     9121 atcaatgaaa aaacaatcat tttttgtcca tctttgactt gattgtcaat cgtgttgttt
     9181 gttaagccaa cacaaactgc accaataatc tcttgatcat aataaatagg gacgaaatag
     9241 cggaagccga accctaacat tccttctttt tcagagaaaa attcttgtcc tccaatcact
     9301 tttttcacat cttcatgact tgagaaagga agaccgatgt aacttgggtc tgcgtgagaa
     9361 tatcgaatgc cttcagggtc catgacaacg ataaaatcta aattcgatgc aatcatgatt
     9421 tcatttgtta ttccttgaat cgcatgtgtc gggtcaccag tctgcagctt tttttggatg
     9481 agtttatcat tggcaacgat tttagcaatg ctagagattt tatctgacac actagatcgg
     9541 ttcatcgaaa gaataaaatt cctgatcaat atggcagaaa caatcaaaga aacgaatgtg
     9601 gagaaacaaa cgacaaggat gatcgtgttc tggagcttta atggtttttt cttaaaaaaa
     9661 ggaaacatag gctacctcga aagaatttat tgtgaaaatt ttaacatgaa aacaaaaatt
     9721 taacaaattg tcaacgataa acacaacctt cctataaaaa acaccactga gtcggtaaaa
     9781 gaagctatac agaattttca aacattggta tgatagacgt agaaatggaa ggaggcgtgt
     9841 atatgatagg gggaatcgca cttgagacga tttattggta tacattgctg atttgtgcag
     9901 gattggcagt cctattgatt atttttggag atgtattcga ttttgatggc ccgcttgatc
     9961 cgatgcttat tatcccttgg ttaactttca caagcttatt tggttttcta ggtgagcgtt
    10021 taacagatca atcatcttta tttttattta ttgctagtgg aggcatatcg atagtgttgg
    10081 tatttttgct taatttctat atattgatgc cgatgaaaca tgccgaatca acgatgtcgt
    10141 cttctgaaaa aactctggaa ggacaagtag ctgttgtgat cacaccgatc cctgtacgag
    10201 gtatgggaga aattcagttg aaaagtgtta ccggttccat tagtcgtcca gcttgttttt
    10261 acagccccca agaacagaca gctgagcggg gaacgaaagt attgatcatt gagatcaagg
    10321 aaagaatctg ttatgtcatg ccttatgaaa gcatgataaa agcttaaagg agcgtgtgtt
    10381 aaatggaaat accagttgtt tttatcgcga tcgcaatcgc agctttgatt ttactgatgt
    10441 tgctgatcgt ctttgtagcg aagtaccaga cagcaaaacc ggatgaagcg ttgatcatca
    10501 gtggaagtta tttaggaagc aaaaatgtgc acattgatga aagcaataac aaaatcaaaa
    10561 ttgtgcgtgg aggtggcgca ttcgtcttgc cagtttttca acgttcgaat cggatcagtc
    10621 tattatcgag taaattggac gtctccaccc ctgaagtgta taccgaacaa ggcgtaccgg
    10681 tgatgtgtga tggtacttcg atcatcaaaa tcggttcatc tgtagaagag attgctacag
    10741 cagcagaaca gtttttaggc aagacaagag aagaactgga aaatgaagca agagaagtat
    10801 tagaaggaca cttgcgctcg attcttggtt ctatgactgt agaagaaatc tatcaaaatc
    10861 gcgataagtt cagtcaaagc gtacaagaag tcgcttctgt agaccttgca aaaatggggt
    10921 tgatcatcgt ttctttcact atcaaagagg tcagagacaa aaacggttat cttgactctt
    10981 tagggaaacc aagaatcgcc caagtcaaaa gagatgcaga tatagcagaa gcggaagctc
    11041 taaaagaaac gcgtatcaaa aaagctcaag cagaaaaaga atcacaagca gctgaattac
    11101 agcgacaaac agagatcgca gaatcattaa aagaaaaaga actgaaatta gcaacctaca
    11161 aacaagaaca agatgttgct aaagcaaaag cagaccaagc ctacaattta gaaagtgccc
    11221 gagcacaaca acaagtaatc gaacaggaaa tgcagattaa aatcgtagaa cgccaaaaac
    11281 aaatcgaact ggaagaaaaa gaaatcacac gtcgtgaaaa gcaatatgat tcggaagtga
    11341 agaaaaaagc agatgccgat cggtacgcta aagaacaaga agcacaagct caaaaagtca
    11401 aagaagtgac tgaagccgaa gcagaacgat tccgtgtgga agcactggca gaagcggaag
    11461 ccaataagac acgtttagct ggacaagcag aagccgaagc agcactagca aaaggaaaag
    11521 cagaagccga agcaaaacaa aagatcgcca atgccttcaa ggaatatggc gaagcagctg
    11581 tcttgagtat ggttatcgat atgctaccgc aattgatgcg cgaagcagca cagccgctag
    11641 gaaatatcga aaaaatctct gttgtcgata caggaagcag ttcaggagaa accagtggag
    11701 cgaaccgagt gacaaattat gccacaaatt tgctttcttc tactcaagaa acactaaaag
    11761 cgacaacagg acttgacttg aaggaaatcg tagagaattt gtcgacacgt gggactacta
    11821 gccaagtaaa tatgtatgaa gcgccaaaag aggcagaaca aaactcgatg aaggaagaat
    11881 aaagtagtac acttcaccgt tcaatcaaaa atggaggaag actttttaaa gttcttctct
    11941 ccattttttt attttattgc aaaagaaaat tagtcctcgc tatttttctt ttgtttttgg
    12001 atcttagctt ctatctcttg taaatttgtt cttagcaatc gttctttctt ttggagttcg
    12061 accaaatctt ctgatagttc ttccttcttt tctgtttcag gaggacgaag aaagaaattg
    12121 ataccgattg caatgaatgt ccctataaaa gtatctagta cacgttggat agcatacaaa
    12181 gcggattcgc cttgagggat gctcaacgca atcagcaaca acgtagctat tgcggaaatg
    12241 ataccggaat tattgttaat accatctgac acgacgatca caataataac tagaatagga
    12301 agcaagatca attctaccaa aaaatcattg tgaaaatagc ttttgacaaa aaaataaatg
    12361 atagctaatg taccacctaa tgtattgccg atgatcctag actttccaaa agaaacggtt
    12421 gtagtcaagt cctgacgcaa agaaaaaaca gcagataatg cagcaatcat cgggattcct
    12481 cgttgagtca aatgaaaaaa caagatacat aaaaaaacag ctaaggcact tttgatcgta
    12541 cgcataccta gtcgaaaacg gccaaattgc atagagattc tcctttttgg tctttttact
    12601 atcataccta aacttttgtc tgatagctag tttttcatta ttcaagtata attaaaataa
    12661 aaaaggggga agtaaatgcg tataggaatg cgaacagtca aaacagtgat cagtgtcttc
    12721 ttttcgatgg tcacagcggg tagcttatcg ctgttgtatt ggccagctgc agggatcatt
    12781 gcattattaa gtgtaggaaa taccaagcgc tctacattgc gtacgggcat gaatcgaatc
    12841 gcagccttta tattagctac ggcaacagca ctgttcagtt tttttgctgt tgggtatact
    12901 atttttggct tcagtctttt tttattcctg ttcatcccgc ttgcctctcg cttcaaatta
    12961 accgaaggga tagtagtaaa ttcggttttg gtcactcatt atttagtgga ggaaaacatg
    13021 tcttggcagc tgatcggcaa tgaagcggga ttaatggcga ttggattagt atttgctttg
    13081 cttgcaaatg tttacatgcc agatcgtacg aagcagctga aggaaaacca aatacagatc
    13141 gaaaaagaat ttcgaaatat tttgagacaa atggcagaat ttcttttatc agaaaataaa
    13201 gaagatgtgc agatcaaatg cgaacattta ctgacattca ttcgcgaaag ccaagaagat
    13261 gccagagaac atcaagaaaa ctactggctc agacagccgc tatactacga aacttacttc
    13321 tctatgagaa gagcacaggc caatgtgatt aaagacatgc tggaaaatct agagagaatc
    13381 cagcaaccag cttattatgg aaaacatatc tatggtttat tgatttatac tgcagaaaca
    13441 ttttcagagt caaatgatgg cagacaaatt ttaacacgta ttgaagaagt ttatgtattg
    13501 tatcggcaaa tgcctctgcc gactagtcgc cctgaattcg aggatcgagc agaattgttc
    13561 cagtttttgc agtcttttaa aagttttatt gaaatcaagg cagaattctc ccaacaaaaa
    13621 atccaaaaat agaggttgtg aattcagcta atattcaggt caaaaagctg atttcacaac
    13681 ctcttcttta aacggtgttc tcctgtcaac tcctcggtca caagtcacaa tattcaacaa
    13741 acatgagaag ctgttttttg aaattgctcc ttgtgactcg gagctgaacg gtaggctcac
    13801 aacctctttc ttaaacggtg ttcaaaagct ttcctctgcg gtaataagtc cagccaaacg
    13861 aaaaatagga ggagctattt ttgtttggct gttcttatta cttgatgcag aacagctttt
    13921 tcacaacctt ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga
    13981 tgacatcttt tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca
    14041 tattctttat ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg
    14101 taccatctgt aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat
    14161 aaccgttctc aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga
    14221 cagcagcaag ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg
    14281 tttcaaaagg aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag
    14341 cgaaagatac agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga
    14401 agaaccggtt tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta
    14461 aagcccccag ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga
    14521 agattgtcat cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag
    14581 ccagttcttt tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca
    14641 atgctgccaa gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga
    14701 tttcagcatc gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt
    14761 gagcgaagtt ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct
    14821 cttgattgaa gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt
    14881 gatatttttt catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt
    14941 attataacaa aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa
    15001 agcagcgatt taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg
    15061 ataaacgtgc atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa
    15121 actgaaaaaa attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg
    15181 aaagatggcg agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact
    15241 gattatgaat ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa
    15301 cgaaacgttg ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt
    15361 agcagcaaag cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga
    15421 taccttgact tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat
    15481 tttcgtctgc cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat
    15541 tgggtgaatg aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa
    15601 caacgaaagg atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat
    15661 cgcagcaatg ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca
    15721 agcagtcgaa cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac
    15781 cagcagcaag gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt
    15841 taaaacagac gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat
    15901 tgatgagtct actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc
    15961 tccagaatat gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt
    16021 ctcagcttac cggtctgtat catcccaaca acaagttttc gaacaaaatg tccaagatgt
    16081 gatgagtcgt caaggtgtat cagaagagga ggcgacaaag attactaaag aaacgattac
    16141 ggagccagga tacagcgaac accacacagg tctggcagtc gatgtcgtcg atcaaaactg
    16201 gtacaacagt tatccttcta ctgtattgga tgcaagttac ggagatcagc ctggagctaa
    16261 atggatcgca gataatgctg cgcagtatgg atttatcgtt cgttatccaa aaggacacga
    16321 agatataact aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc
    16381 tgagtatatc accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa
    16441 gtaggaattg atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg
    16501 gccagcagtg gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa
    16561 tctgtcttct ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt
    16621 tattgaacgg cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag
    16681 tatcatttta ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa
    16741 atacaataat cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac
    16801 aaaggaattt gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc
    16861 atgggaacag tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc
    16921 tgcgctgtat gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca
    16981 agatgcaggt tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac
    17041 gtatcaattg aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa
    17101 tttacgccag aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt
    17161 ttagaagcaa gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg
    17221 gatattgcga tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca
    17281 cctcatccag cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt
    17341 gtaggtggtg gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg
    17401 caaggttcgt taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt
    17461 tgtcgagtag ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat
    17521 gaaaagttag cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa
    17581 gcagtacgcc attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca
    17641 acggaagaaa gtattgctga gaccattcaa gcaggagcaa atgccatcac atatacgcca
    17701 ccaagtagcg gagagctttt tagtcgtaaa atggaaaaat atcgggaaac tgagaaaaag
    17761 aatcatgaat aacaggtgag aatcaaaaga cgaggaagag cagatagtgg ctcttcctcg
    17821 tcttttgtac tccagtacta tttgttcaga attcaaaaaa aagagaaatt tttttgtttt
    17881 tttacctgta atgtacataa gaagagttaa aaccttttgt tattattagc attctaacaa
    17941 aaaaatgtct tgatttattg aagcgctttc tatagaatga aaaacgaata aaaactaaaa
    18001 aaggcacccg acaatttagt agcagctaaa ttgtcggggc ttgaaaagtc agcgggaact
    18061 gaccaatcaa gttgccatag tcaatgcttg tgcatcgacg tgtatattgt acctaatttc
    18121 atacagaatt actagttatt tttctgtttt ttagggaaga gcgcacacga agaagcatgc
    18181 ttatttagct atgtcaagga tattgggcag ttgtctgata tccttttttc tttgcgtaat
    18241 tatcctccca tcattgaagc aagaagtgtt tttagcgatg aaagaagaat cagcactatc
    18301 taatttttct tgaaagtatt gagttgttca tcctaggcag aacgataagg caagtcttgg
    18361 tttttattcc ccttgaaaaa gatcgtttaa taaatatgtt ctgtataaaa atacagtata
    18421 ttgaaaaata acaatggaag tagaggtgca gagaatgaag aaaagaatga gaatcatctt
    18481 agctttaagt gttatgttat tcttcttgac ggcttgtggg caagaagatg acaaaaaaat
    18541 cacgctgaac atggcttggt ggggttcgca agtccgacac gatgcaacag taaaagtaat
    18601 cgagctttat gaagaaaaaa atccccatgt gacgatcgat tatgaatttt atgattatga
    18661 aggatattac acaaaattag gtacactagc agcatcagat gatatctggg acattttcca
    18721 actgaacaat caatttcctc aatacatcaa tcagattgaa ccattagatg aatatgtgga
    18781 gcaagggata attgatattt ctaatgcaga tgaatcctat ttagcaacga cgacttacga
    18841 cgggcaatta gttggactgt ctaacggggt gaattcgttt gcgattgctt acaataaaaa
    18901 tatatttgaa caattaaata tccctgaacc agcttcaaat tggacatggg atgaatttga
    18961 acaaatcagc tatcaaatca ctgaagaatt aggaatctat gctatttcaa gattcgaaga
    19021 ttttatagct ggctgtatta ttcaaatacc acaagttgag aaaggattga atttttataa
    19081 tcgggaagat ctatctaaca gcttgggatt tgaaaatcca gactatctaa cagatttttt
    19141 ccagctgcgt aaaaatctgg tagacaaagg ggcccatcca gaaccaggtg gtggagctgc
    19201 aagtacgacg gatctgtctt ttcaggcggt tagggataga gaagcggcaa tggtgtttct
    19261 atcaagtaac cagttgacag aaatattgtc aggggctcct gaagatatgg agattcagtt
    19321 gattaatcca cctcgcagaa aagcagatgg agaatctggt atccctttac gttcttcgca
    19381 aatgctgagt atggcaaaaa gctcagccca taaagaagaa gctgctaagt ttattgattt
    19441 ctttcaaaac agtgaagaag ccaatgaaat tttaaaagga gaacgtggtg taccaatcat
    19501 gtcgaatatt cgagaacagc tcgctcgtgc agatgatgca actttgcaag cgacatttga
    19561 ttatttagaa atgatttccg atatggacaa cggcgaagtg aatgtctttg atagtgcttt
    19621 gaatccggaa attcaagacc aatacaatct tttagttgaa aaagtcattt atgaagaagt
    19681 aacagcagaa ggaaggcatg aaaaaagaaa aactaaaacg gttcttggat caagacaatg
    19741 tggtcggtta cgtatttgct tctccattca tcctaggatt tctattgata acagtgatac
    19801 cgatgatctt gtcactttat tattcgttca ccgattatcg tttgggaaat ccagtctcat
    19861 ggatcggctt ggataattat ataaagatgc tgagtgatgc ccgttttttg aactccatcc
    19921 gagtcacttt tctctatgta tttgcatctg tacctgtgaa actgatcttt ggtctattga
    19981 ttgcttatct tttaacgagg aatatcaaag gaacgacttt ctttcgttct ttgtattatg
    20041 tgcctagttt gatcggtggc agtatttcta tcgctctagt ttggcgggag ttgttttcta
    20101 aaaacgggtt agtgaatatt ttcctggaac gattaggcgc acctgcgctt tcctggttta
    20161 gcgatcaaaa actggtcatg atccctcttg ttttgatgtc tgcttggcaa ttcggttcgg
    20221 caatgatcat ctttgctgca ggtatcaaag agatcccagg ctcgtattat gaagcggcag
    20281 agatcgatgg agcgaacaaa tggcagacgt tttacaagat cacgttacct cttctttcgc
    20341 cgatcatttt gtataacttg gttatgcaga caatcgcggc gtttatgact tttacgcagg
    20401 catttatcat tacccaaggt ggtccgaatg atgccacgaa cttttatgct ctttatgtgt
    20461 ataaccaagc tttttctttt aattctatgg ggtatgctag tgctatttcg tggatcatgc
    20521 tgttgattat gggagctgtc acctttctga ttttcaaatc atcaaaaaaa tgggtcgttg
    20581 tgtgaatgag aggaggaaga aatgaataat acagtaacga aatggtttca atacggtttt
    20641 attttgctat taggtctagt catgatttat ccggtcttat ggatgatcgc aggttctttt
    20701 aaaacggatc aagagattat gagtggttcg ctgaatttga ttccagagac ttttcaatgg
    20761 gaaaattacg caaaaggatg ggcggggttt gctggaattt cattttttac tttctttagg
    20821 aattcgtttg tcatctctgc agtttcgacg atagggacag ttctgagctc gacttgtatt
    20881 gcttacgcat tatcgagaat cgatttcaaa ggaaaaagat tctggttcgt cgtcatgtta
    20941 gtaacgatga tgattcctgc acaagtgatt ttgattccgc agtttattat ttacaatcgg
    21001 ctgaatttgg ttgggacgta tgtgccgctg attttaccac atttttttgg acaagcattt
    21061 ttcatttacc agatcatgca attcatggtc agcatcccca aagagttaga tgaatcagcg
    21121 attattgacg gatgcagcaa gtatagtgtc tttaccaaaa ttatttttcc tttgctgaaa
    21181 ccttcgatca tcacgacgat catcatccag ttttattgga aatgggacga cttcatggga
    21241 ccattgattt atttaaacaa gccgagatct tacacggtat cgattgccat caaattattt
    21301 gcagacgctg gatctagtac gacttacgcg agtatgtttg cgatgtctac gttgtcgctg
    21361 ctaccggttt ttctgatttt cttattcttc aataaatatc ttgtccaggg gatcagtaca
    21421 agcggtctga agggctgaat atgaaaaagc caatagaaga gatgtttctt tacaagctca
    21481 actactatgt ctgtcggatt tttttaacca gtcttatcag taatgctctt ttcgctttag
    21541 ttctaggatg tttgttcact ttaggattag tgctgtctgc tcgtgttgtc tatctgattt
    21601 attttttgat gatgtatcct tcttattatc tatctgtcca ttacttggca gaaaaaaagg
    21661 tatgttggag gaaagggaaa atcaaacgga tgtgtttcat cattacgttg tttcagctgt
    21721 ttgtcattat ttttgcagtt gattggtatt tattagtggg aaagattgga gcttggtatc
    21781 tgattccttt tctattgtgt ctgttgcttt ttgtatccca tagttttttt agtacgctaa
    21841 ttgatatctg caaagaagat aaagcagata gtcttgttta tttgatcaaa aggaatatct
    21901 ggctgttgtc aaaaaacgtc aaggggtttc tgtggtattt atgccttttg tatggactgt
    21961 tctggtgtat acagcctgat tttttactca ctgcattact gtatccaggg ctgccatttg
    22021 tcacagtatt ttgcttaaaa gaaaaggaga atgactttaa atgaagaaaa aatatgtaca
    22081 agtcggactt ggtgggagag cgcgtttttt ttatgaggca attgcctcta cgtatcatca
    22141 aacttgtgaa ctagtcggtt tttgtgatgt atctcaaaca aggatggatt atgcaaatcg
    22201 gatacttgtg aacaagtatg gacagaaaaa agtaaagaca tgtatgccat cacaatttga
    22261 gcaactactg gatgacgtac ggccggctga agtcattatt acgtcagtag atcgaacgca
    22321 tcatgattat atcattcgtg caatgaaaaa aggatatgat gttatttgcg aaaaacctct
    22381 gactatcgat gaggaaaaag ctcaagaaat aataacggtc caaaaacaga cgggaagaag
    22441 cctgcgagtc acttttaatt atcgttatgc accgtatcac acgaagataa aggaactgat
    22501 agaaagtgat gtgattggtg atgtgttttc tatccacttc gaatggctgt taaatacgca
    22561 acacggggca gattatttca gaagatggca tcgaaataag cacaatagcg gcgggcttct
    22621 tgttcataaa tctacccatc attttgatct agtgaatttt tggttgaaca gccgtcccca
    22681 aaaggtattt gcattcggtg gattaaactt ttatgggatg cataatgcgg agaaacgcgg
    22741 agtagaaata ttttacagtc gatgctatgg aagcgaacta gctaaagctg atccatttgc
    22801 tatagacctg tcagccaatg aggaattgcg agaaatgtat ttagaagcag aaaaagacag
    22861 tggatatatt cgtgaccgca gtgtattttc cgatgatatc tctattgaag atacactagg
    22921 cgtaacggtc aaatatgaaa ctggtgcgat tttgacgtat tcgctaaacg cgtatatgcc
    22981 gtgggaagga ttcaatgttt ctttcaatgg cagcaaagga cggatcgaaa tgaaagtgat
    23041 cgaaaagtct tacatcaatg caggtggaga taaaaaggat gaaggagcag ccgttcttca
    23101 gtccatccat gtctatccga tgttcggtga gccatatgag gtaatcgtcg aacaagaagc
    23161 gggcggacat ggcggaggag atccgcgttt attagatgat ctatttggtg aagaaaaaga
    23221 agaagatccg tggaatcggg cagcaactca cgtagatggt attttgtcga ttttgacagg
    23281 aatcgcagca aatcattcta ttgcttcagg aaaagcagta gtgattgacg agcttgtcag
    23341 cttaaaagaa taagaataga tttacggaaa ttacttcccg tagtttataa aatcttgcct
    23401 tattgccgat aggctgtctt ttgaacacta tttattcgga aaaaggggct atgacatgct
    23461 ttttgtcatg ccttcttttt atgtgggctg cttttttaga taggagaaaa taaaaataac
    23521 tatttaaacc cgaacaaaaa ggaataatgc attaaaaatc attagatttt acaaaaaaat
    23581 aagtaaacca cgttgtcagt ttcagaaagt agtgttagac tgttttcaat atttgaaaca
    23641 acatttttta ttcaaatatg gcaaactgcc ttcacgtgca aaacagttgc aagaaaatga
    23701 atttacacta ttattagttt tacataccta cttatatatc gtcataatat ggatgacagt
    23761 ttctagccag taccgtaaat gctggactat aagtaaaaga ttggctattt cacccttctt
    23821 ttcttatgat aaagtggagg gtgtttttct ttaacagaaa catagtaggt ttttgcacaa
    23881 aagcggttat ttcggtatta ataaatgata aaggagcaaa taaagtgaaa gaattagttg
    23941 aacgtattca aaaagacgga cgtatattag gcgagggcgt attaaaagtc gacagcttcg
    24001 tcactcacca agtagaccct gtattgatgg aacaaatggg aaaacggttt gcagaagtat
    24061 ttaaagaaca aggaatcacg aaagttgtga ccattgaagc ttctgggatc gcaccagctt
    24121 tatttgctgc aagagaatta gatgttccaa tgatcttcgc tagaaaagca aaaagtttga
    24181 cgatggatga agaattattg acagcttctg tttattcttt tacgaaacaa gtgacaagta
    24241 cgatttctat ttctcgtaaa tttttatctt cagaagacaa agtattgatt atcgacgatt
    24301 tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt atgccagcaa gctggagcgc
    24361 aagtagaagg aatcgggatc gtcatcgaaa agtctttcca agatggacgt cagttacttg
    24421 aagaaatggg attgagagtc gtttctcttg cgcgtattgc ttccttgacg aatggacaag
    24481 ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa acacaaaatg gaaaagctgc
    24541 ggttttaggg ctgcagcatc tgctagcgat gtatgcagga gcagtagcgg tgcctctttt
    24601 gatcggtaca ggactaggct ttaatcaaga acagatgact tatttgattt caattgatat
    24661 ctttatgtgt ggattagcga cattattgca attgacagtt aaccgatttt tcggaattgg
    24721 cttgcctgtc gttttaggat gtgcgatcca agcagtagct ccactgatct tgatcggaac
    24781 caatgaagga gtaggagcta tctacggatc tattatcgct tctgggatct ttgttgtgtt
    24841 agtttctggt gtcttttcta aaattaaaaa actattccct cctatagtaa caggaacggt
    24901 tatcaccgtt attggtttga ctttgattcc tgtagcaatc gagaaaatgg gtggaggtaa
    24961 tgcaacagca gctgattttg gtgacaaaac aaatctgcta cttgcatttg tgacgattct
    25021 cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt cgttcaatcg cggtattgat
    25081 tggtttagtc ggaggaagca tcttagctgc tttcatggga atgattgatc tgagcgcgat
    25141 tggagatgcg ccgttgttcc atgttccaca acccttttat tttggtaaac caactttcga
    25201 cgtatggtcg attttactaa tgatcatcat ttccatggtc agcatggtag aatcaacagg
    25261 tgtatatttt gcacttggtg acattactgg gaaaaatgtt ggagaagaag aattgaaaaa
    25321 gggatatcgt gcagaaggac ttgccgttat cttaggcggt attttcaata cattccctta
    25381 tactggcttt tctcaaaatg tcggattggt tcaactttcg ggaatcaaaa caagaaaacc
    25441 catctatttc tctgcattct tcttgattgt tttaggatta ttccctaaaa tcggtgcgtt
    25501 agcacaaatc atccctgagc cggtacttgg tggaggaatg ttagtcatgt tcgggatggt
    25561 ggcagtccaa ggaatccgta tgctgttgga agttgatttc agcaatgata aaaatctatt
    25621 gattgttgct gtttctgttg gatttgggct gggtttcaat gttacgccga cattattcag
    25681 tcaaatgccg caaacattgc aaatgttcac tggaaatggc atcgtaatga gtagcattac
    25741 ggctatcata ttgaatttga tctttaatgg attgaaaaaa gatgaagaaa tattggaaga
    25801 agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa tcagcgtttt gacctgattg
    25861 ttagagaaac aaacggaaaa atagttcctc gtattttgcc gtttgtttga gctaataccg
    25921 agggtcagct gattgaacac cgtttatcca taaaaaggaa tgtgacctaa cttttgtcac
    25981 attccttttt ttcatcttta ataaacacga acaataaaat gtttttcttt gttaacattc
    26041 gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat gaatgtagaa aaactgggag
    26101 gaaaaacaat gacagcaaaa atctctgtga ttatggggag tacttcagat tgggaaacaa
    26161 tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc ttatgaaaaa aaggtcgttt
    26221 ctgctcatcg aaccccagat ttgatgtttg aatatgcaca aaacgctcga aaaaaaggaa
    26281 tcaaaatcat tattgcagga gcagggggag ccgcgcatct gccgggtatg gtcgcatcaa
    26341 aaacaacttt gccggttatc ggtgtaccgg tccaatcacg aacattaaat ggtttggatt
    26401 cgcttctttc gattgtccaa atgcctggag gagtacctgt agctactaca gcaatcggaa
    26461 aagcgggagc aattaatgct ggactacttg cgatacagat gctttccatg tatgatttag
    26521 aattggaggc taaattggct gaaagacgtt caatgttggc taaaactgtt atagaaagta
    26581 gtgatcaact tggttaaacc tttgttgcct ggttcaacga tcgggatcgt cggtggcggt
    26641 caattaggtc gtatgatggc gattagtgca aaagaaatgg gctttaaagt aggtgtgctg
    26701 gatcctgtga gcgattgtcc agccgcacaa gttgcagatt ggcacattga aagtgtctat
    26761 gatgacacct ttgcgctgaa agagcttgcc agaagaacag atgtcattac ctatgaattc
    26821 gaaaacgtca gtgtagaggc gttaaatgcg attttaccca tgtcatttat tcctcaaggg
    26881 acagacttgc ttgcgattac gcaagaccgc ctgttagaaa aatctttttt ggaaacgaat
    26941 aacatcgtga ttgctcctta tgcaacgatc gtcagtccga cggatatcca agatgcgatt
    27001 gacgggatcg gctatccttg tgttttgaaa accactcgag gcggctatga tggcaagggg
    27061 cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca tggacctttt aagagaagga
    27121 acctgtgttt tagaagcatg gatcccgttt gaaaaagaga tttcgatcat ggtggcagga
    27181 aatggtcaag gagatttcac gacttttccg gtcgtggaaa acatccacca caacaatatt
    27241 cttcatgaaa cgattgctcc agccgcgatt gatcaggatg tgatcgagga ggctgagcgt
    27301 attgcccgtg tgattgcaga agcagtgagc ctttcaggtg ttttaggggt agaaatgttt
    27361 ttaacaaaaa caggcgggct atatgtgaat gaattagctc ctcgtcctca caattctgga
    27421 cattattcta ttgaagcttg ttctatgagt cagttcgatg cccatattcg aggtatttgt
    27481 ggttggccat taggagaaaa agtacgtttg ataagcaatg cagtaatggt caatgttttg
    27541 ggagaacaac tgctggaaag ctatcgcttg attgagaata agccagactg gcaattccat
    27601 ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg gacatatcac tatcttgaca
    27661 gaagacatcc agcaaacgct ggaagaaatc aaacaaacga atatttggga ttagaggaga
    27721 aaaaacatgt tagaacgtta tacacgccct gaaatgggga atatctggac ggaagagaac
    27781 aaataccgtg cgtggctcga agtcgagatt ttagcagatg aagcttgggc agaacttggt
    27841 gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg catcatttga tatcgatcgg
    27901 atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg cgtttacacg agcggtttct
    27961 gaaacattgg gagaagaaag aaaatgggtc cattatggtc taacaagtac ggatgtcgta
    28021 gatactgctt atggctatct catcaagcaa gcaaatgata ttttacggga agacttgaaa
    28081 cgttttacgg aaatcattgg cgaaaaagca aaagaacaca aatatacagt gatgatgggt
    28141 cggacacacg gtgtccatgc tgaaccaact acttttggat tgaaacttgc cacatggtat
    28201 tcagaaatga aacggaacat cgaacgattt gaacatgcgg caaaaggagt agaagcagga
    28261 aaaatctcag gagcagtggg aacgttcgca aatatcccgc catttattga agagtatgta
    28321 tgtaaacaat tggggatccg tccacaagaa atctctactc aagttctccc tcgggatcta
    28381 catgctgaat atttctcagc gatggcgctt attgctacaa gtattgaacg ttttgcaaca
    28441 gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag aagaattttt tgcgaaaggt
    28501 caaaaaggtt cttctgctat gccgcacaaa aggaatccga tcggttcaga aaatatggct
    28561 ggtctagcca gagtggtgcg cggacacatg gtcactgcct atgaaaatgt cacattatgg
    28621 catgaaagag atatttctca ttcttcagca gaacgaatca tcttgcctga taccacgatt
    28681 ttattgaatt acatgttaaa tcgttttggc aatatcgtta aaaatctaac ggtctttcct
    28741 gaaaacatga agcggaatat gtctgcaaca tttggtttga tttatagcca gcgcgttatg
    28801 ctgaaattga tcgataaggg aatgacaaga gaagaagctt atgatcttgt tcagccaaaa
    28861 actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc tgttagaaga agatccaacc
    28921 atcacatcgg tattatctaa agaagaaata gacgatgcct ttgattatca tcatcatttg
    28981 aaaaacgttg acgtgatatt caaacgagta ggtttggaat aaagaagagt gagttgcaaa
    29041 attcaattaa aatagaatag caaaaataac caaaaaagaa gcagatccgt tagcggttct
    29101 gcttctttta gtttattata ttttcttatc aataatagga acctgatttt aaaagcggct
    29161 acctctttac cgtgcctctt tacatacaaa aaatagagga attaagcgta aatggcttgg
    29221 ctattataac taaaatgctg gttatggaga tgttgcagca agttttcgag aaaagcgtat
    29281 tccatctctt gtccattgaa taagatctgg atacgataat gatttttgaa acgcttctgt
    29341 tcatgaatct gactgattga aagttcgcct ttgtgttctg tctgataaag aaaagtcctt
    29401 ttacggcatt cagcacagcg cttggttgct cggcggattt ttcggaatgt ataaaaacta
    29461 gtatctttat tgcataaata aacgtctttg atcgacagca aatcattata tagacaggaa
    29521 cgttcttcag ctgttaattc tgataaaaaa agctgacctt gttttattgc atcttccatc
    29581 ggcaagtgcc tcctcgaacc cattatccaa atttttagtt aattctaata taccatcatt
    29641 aagtataaat gtaaacgctt gttgatggta taagattttc gtttctgata aaacacgaac
    29701 attaaaagaa tctaatgcct taaatgtaca tatttttgtt gatttataaa aagatgattg
    29761 ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa
    29821 aaggagagac gtacatggag aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat
    29881 atcttacttc tgatgagaac ttgatttgga tagaatattt agatcaagca actgcgttga
    29941 atggcgcgag gaaagaccag ataagtggaa aaggagaatt gaataatcaa atcacaagtg
    30001 caattttccg ttatctgact caaagaggaa taaaaaatca ttttattgaa caattgtcta
    30061 aaaccgaaca attagcaaaa aaagtacaaa ttatccctct ggaagtggtt atccgaaata
    30121 ttgcagcagg aagtttctca aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac
    30181 caatcattga attctactat aaggatgacc agttggatga tccatttatc aatgaagagc
    30241 atatctattt cttacaactt gcaaaaccgg aagaaatcag tgagctgaaa gcccaagcgt
    30301 tggctattaa tgaaattttg accgaattat ttgctaaggt cggtttggtt ttagtagatt
    30361 tcaagctaga atttggccgg tcgaatggac aaattttgct tgcggatgaa gtaacaccgg
    30421 atacttgccg attatgggat gaacgtacga atgaacatat ggacaaagac gtttaccgca
    30481 gagatctagg tgatttgatc ccagtctatc aagaagtata taaccgtcta tcggaactaa
    30541 ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta tatgtaacct ataaagattc
    30601 agtgttagat cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac
    30661 aatcgaagat atccgaatag gaaaatactt tgagatacaa cttgctgatg cagaagaacc
    30721 aatcgataca gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa atatggaaac
    30781 ataccgatat gaaatcaaaa aaatggagga agtataacat gaaatttgcg gtcatcgttt
    30841 ttccaggttc aaattgtgac gcagatatgt tatgggcagt tcgcgagatc atgggcgctg
    30901 atgccgaatt tgtacggcat gatgagtcct ctttagaagg atttgatggg gttttactgc
    30961 cgggaggatt ctcctatggt gactatttga gatgcggtgc gattgctcgg ttttctacaa
    31021 tcatggaaga agtgattcgt tttgctgaag aagggaaacc agtattcggt acatgcaatg
    31081 gattccagat attgacagaa gcaggacttt tgccaggtgt actgagacga aatgagtctt
    31141 tgcattttat ttgcaaaact gtaccattga aagtcaaccc caagacggcc tttacttcag
    31201 agtatgaagc cgacgaaata atctatcttc cagttgctca tggggaagga aattatttct
    31261 gtgacgaaga aacacttcaa gaactaaaaa aaaataatca gattgccttt acttataatg
    31321 acaacatcaa cggcagcgtg gaaaacattg cagggattac gaacaaagca gggaatgtgt
    31381 taggaatgat gcctcatcca gaacgtgcag tagaagactt gctaggatca gaagatggga
    31441 aacggttctt tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat
    31501 tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa tggggattga
    31561 ctgatgaaga atatcgaatg atcgaagaag atatcttagg aagaatgcct aattatacgg
    31621 aaactggact gttttctgtt atgtggagcg aacattgttc ctataaaaat tctaaaccgg
    31681 ttctacgcaa atttcctact agtggacctc atgtgttgca aggccctgga gaaggcgcag
    31741 gcatcgtcga catcggtgat ggactagcag ttgtcttcaa agcagaaagt cacaatcatc
    31801 cttcagccgt ggagccttat gaaggagctg cgacaggagt tggcggaatc atcagagata
    31861 ttttcagcat gggggctcgt ccaattgcgt tgctagattc attgaggttc ggcgaattag
    31921 ataatccaag aacgaaatat ttactagaag aagtagttgc aggtatcggc ggatacggca
    31981 attgtatcgg cataccgact gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa
    32041 acccattagt caatgcaatg tgcgtaggtt tgattgaaca caaacatatc cagaaagggc
    32101 aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta
    32161 tccacggagc aacttttgcc tcagaagaat ttaatcaaga agaagaacag caacgttctg
    32221 ctgtacaagt aggcgatcct tttatggaaa agctgctgct tgaagcgtgt ttggaattaa
    32281 tcttgcatca ttcggattct ctaataggta tacaggatat gggagcagca ggacttgttt
    32341 catctagtgc ggaaatggct tcaaaagctg gctcaggact tatattgaca ttagatgaag
    32401 tcccacaaag agaaactggg atgacacctt atgaaatgat gttatcggaa tcacaagaac
    32461 ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta tttcagaagt
    32521 atgatctaga tgcagtcact atcggtaaag taacagacga cgggcaatat cggctatatc
    32581 atcaaggaga agaagtagca aatctgccag tcgatgcatt agctgaagat gcaccagttt
    32641 atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta gctccttatc
    32701 agccagttat cgaagaacct ggacaagtat tgcttgatct gctgcagcaa ccgacgattg
    32761 cttcaaaacg ttcaatctac gaaacttatg attcacaagt acaaaccaat acagttgtcc
    32821 gtccaggaag cgatgctgct gtgatgaggg ttagagggac gaataaagcg ttagctatga
    32881 ccacagactg caacgcgcgc tatctgtatt tggatccaaa aatcggagga caaatagcag
    32941 tggcagaagc tgctagaaat attattgcta gcggtggtaa gcccttggcg atcaccgact
    33001 gcctgaacta tggttctcct gataaaccag aagttttttg ggaattatcg acttcagctg
    33061 acggaattgc tgctgcgtgt gaaacgttag gaactcctgt tatttcagga aatgtctctt
    33121 tatataatga aacggatggt caagcaatct atccaacacc aatgattggt atggtcggat
    33181 tgattgagga tcataaacat atcactactc aggaattcaa aaaatcaggg gatctaatct
    33241 atatattagg taagacgttt gcagattttg atggcagtga attacaaaag atgcagcttg
    33301 gaagaatcga aggtgtgatc cgaaactttg atctatccat tgaaaagaga aaccaagagt
    33361 tagtattgac agccatccaa aacggattga ttgaaagtgc tcatgactgt tcagaaggtg
    33421 gcttggcaat tgcactggca gaatcagctt tcaaacatca gctagggata tctgtacagt
    33481 ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc aagatttgtg ttgacagtcg
    33541 caccggaaaa taagacacga ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta
    33601 aggtgacgga tgaagcgata atcgaaattt cagcaactga cggtcagatc aaaatagaaa
    33661 ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg tcttatgaag tgaagagttt
    33721 aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca gacgccgcta gtgtggcata
    33781 cttcgggctc catagtcttc agcatcgagg acaagaaggt gcaggtatcg tgtccaatga
    33841 tcgaggaaag cttaaggggc atcgagattt ggggctgctt tctgaggtat tcaaagatca
    33901 aagaaaattg cagcagttaa caggagaagc tgcaatcgga catgtacgtt atgcaacagc
    33961 aggcaatgga agtgtggaca atattcagcc ttttctattc aaatttcatg atcagtcgat
    34021 cggcttagcg cataatggta acttgatcaa tgctcgttcc cttagaaaag agttagagga
    34081 aaatggggcg atcttccatt ctaactccga tacggagata ttgatgcatt tgatccgacg
    34141 aagtgaagaa acagattttt taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg
    34201 ctttgcctat ttattaatga cagaaacggc aatgattgcc gctttggacc caaatggttt
    34261 tcgtccatta gcaatcggac aaatgaaaaa cggagcatac gtgattgcct cggaaacttg
    34321 tgcattagaa gttattgggg cacgttttat aagagatgtc cagcctggtg agatagtgat
    34381 catcaatgac gaaggaatcc agattgattg ctttacgaag gacacgcagc tttctatttg
    34441 ttcgatggaa tatatctatt ttgccagacc agattcaaac atcgcaggga tcaatgtgca
    34501 tactgcacgg aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa aagcagatat
    34561 ggtcgttggc gtacctaatt cttctttgtc agccgcaagc ggttatgcgg aagctagtgg
    34621 aatcccttat gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc
    34681 aacgcctgag ctaagagagc aaggtgttcg aatgaaatta tctgctgtac gaggagtagt
    34741 ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt cggggtacga caagcagacg
    34801 gatcgtccat cttttgaaag aagcaggggc aaaagaagtc catgtgagga ttgcttctcc
    34861 tcctttgaaa tatccatgtt tttacgggat tgatattcaa acgcgtaaag aactcattgc
    34921 ggcaaatcac acgattgatg aaatcaaaga atgtatcggt gcagattctt taggtttttt
    34981 aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt attcaggatt
    35041 gtgtatggct tatttcaacg gagattatcc gacgccgtta tatgactatg aagaaaaata
    35101 tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga agggagtata
    35161 tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag
    35221 tagtagaacg gatcaagaag catgtcaaaa aaacggaacg tttaggtatg atgggcgcta
    35281 taggaggatt cggcggttgt tttgatttat cggtctttca attaaaagag ccagtccttg
    35341 tctcggggac cgatggcgtg gggacgaaat tagcagtggc aatcgaagca gacaaacacg
    35401 atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga tatcttagca caaggcgcag
    35461 agccgttata tttcttagac tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa
    35521 atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg attggcggag
    35581 aaacagctga aatgcctggt atgtactcag gtaatgatta tgatctagcc ggatttgctg
    35641 tagggatagc tgaaaaatct caattattga caggagaaac gatcaaccca ggagatgtgt
    35701 tgcttggaat agcatctagc gggattcatt caaatgggta ctctcttgtt cgcaaaattt
    35761 tctttgagca gcagcagttt tctttatcat caaaactgcc agaattgaaa aaaacacttg
    35821 gcgaagaatt gcttgagcca acgaaaatct atgtttcttc cttgcttccg ctgatcaaag
    35881 aaaagaaaat aaaaggagct gcacatatca ctggtggagg gtttgtagaa aatatcccgc
    35941 gaatgttacc agaatctatg gctgcacaaa tcaaacttgg ctcatggccg attcttccaa
    36001 tattcggctg tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat gaaatcttca
    36061 atatgggcat cggtatggtc ctggcagtcg aagaatcgaa agtaaaagaa atagtacacg
    36121 aaattgagca agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac
    36181 atcgtgtggt gtttatagga gaagaacgat gagaatcgct gtttttgctt ctggaaacgg
    36241 aagcaacttt caagcgctag ccgattatct atcgaaaaaa ggattggaat cttcgattga
    36301 ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt
    36361 accggcagat tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat
    36421 tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta ttagctggat atatgcgaat
    36481 cattggaccg gtattgctga aaaattatga taaaagaatc atcaatatcc atccgtctct
    36541 tttacctgcc ttccctggtt tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga
    36601 aacgggtgtc acaattcatt atatcgacca aggtgtagat actggaccaa tcatccggca
    36661 ggaaaaagtg agaattgaac aggaggacac gttcgattct ttagaagaaa aaatccatag
    36721 agtagagcat cgaatctatc cagaagtgat tagtgaaatt attgaaaatg gaggctatct
    36781 gaaatgacaa gagcattgat cagtgtatca gataaaactg ggattgtagc attcgcccaa
    36841 ggtttgattg aacaaggagt agaaatcatt tcaacaggag gaacaaagca tgcgttggat
    36901 aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga aatgatggat
    36961 ggccgtgtca aaacacttca tcctaaaatc catggtggtc ttttaggaag acgcgatctc
    37021 cctagccata tggaggcaat ggaaacggaa aatatccaac caatcgactt tgtttgtgtc
    37081 aatttatatc ctttcaaaga aacgattatg aaaccagatg tcgaaatagc agaagctatt
    37141 gaaaacattg atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt
    37201 gtcacctctg ttgttgatcc atctgactat gaagaagtac tttcagaatt acgtcagtat
    37261 gggcatactt cactttcttt cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg
    37321 gcatatgatg cactgatcgc tcaatactta acggattggg tggaagaaaa agaaccggaa
    37381 aaattgacac ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa
    37441 gccgctgcct tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa
    37501 ctccatggga aagaactttc ttataataat atccgagatg cagatgctgc gctacggatc
    37561 atgaaggaat atgacgagcc aacggtagtc gccttgaaac acatgaatcc ttgtggtatt
    37621 gggacagcat ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca
    37681 atctttggtg ggatcatcgt tttgaatcga gaagtagatc tgccaacagc agaacagatg
    37741 cataagctgt ttctagaaat catcattgcc ccttcttata cacaagaagc tttggaatta
    37801 ctgcaaaaaa agaaaaacat ccgattactg acagtagact ttgcgcagga agaaaatcaa
    37861 aatcgagaag aaacggtctc agtcttaggt ggacttcttg tacaagatca agatctggca
    37921 atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta agccgactcc agcagaagca
    37981 aaagcattag cgtttgcttg gagagctgtc aaacatgtga aatctaatgc gatcgtactg
    38041 gcaaacgaac gtcagacagt aggaatcgga gccgggcaga tgaatcgagt cggttcagta
    38101 aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg cagttttagc tagcgatgct
    38161 tattttccaa tggatgattc ggttgaatat gcagccaaac atggtataaa agcaatcatt
    38221 cagccaggag gaagcatcaa agaccaagat tctattgata tggcgaataa atacgatcta
    38281 gcaatggtct ttactgatgt tcggcatttt agacattaga gaggagtcgg ataaatgaac
    38341 attttggtga tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact tttagccagt
    38401 ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc
    38461 aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa tccattttgt taagaaagag
    38521 gctattgatt ggagtatcgt tggtcctgaa gttcctttgt taaatgggat cgtcgatgat
    38581 tttatggcgg aaggattgaa aatcttcggt ccgacaaaag cagcagctct gatcgaaggt
    38641 tcgaaaagtt ttgctaaaca attaatggat acttatcaaa tcccgacagc taaatccaga
    38701 agttttgaat gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc gccaatcgta
    38761 gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa
    38821 gcgctggatg cggcagaaca aatgttgaac cagcatcgat ttggggatag tagcaaaaaa
    38881 atcgtcgtag aagagttttt ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat
    38941 accaaggtgt atccgatggt catttcgcaa gatcataagc gggcatttga taacgataaa
    39001 gggccaaata caggcggaat gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg
    39061 attgaaaaag ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa agaactagga
    39121 cgctctttca cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg accaaaagtg
    39181 atcgaattca atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc tcggttgaag
    39241 agtgactttg cgcaagtaat cgatgattta ttagagaatc gtacagtaga gttaaagtgg
    39301 cagcaagaag gatacaatgt aggcgttgtg gttgcagctg ccggttaccc cgaagaatat
    39361 gagacaggaa tggttctacc agattttctg gaagaagagc tttctgttta ctatgctgga
    39421 gtttcctcaa ataacggcaa actagttgga gcaggaggac gtctttattt agtagaagca
    39481 tacggtgaga cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat ggataaaacg
    39541 acagatggaa cattttatcg tacagatatt ggagaaaaag ctgccaatag cgaactgatc
    39601 gtgaagtaaa tcagctgctt ccatttataa aaaatacaac aaacataatc ggaagcgccg
    39661 attttagtga agccttttca taaagagtgt ggtacacttt tgttgtgtat ataaataagg
    39721 aggagttcag atgaaaaaaa ttcaattagg tacttctgat cagaaagcag ctgcagtgat
    39781 cttaggctgt atgcgtatca acaatgcgga agatccagta aaagtgattg agacagctta
    39841 tgaaaacggc attgattttt ttgaccatgc agatatttat ggaaacggtg aatgtgaaga
    39901 aatctttgca gatgctctag caaaaacatc aatcaaacga gaagatcttt ttattcaaac
    39961 aaaatgtgga atcgttccag gtgtgatgtt tgatttttct aaaaaacata tcattgaatc
    40021 tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg gatgctttac ttcttcatcg
    40081 tccagataca ttggtagaac cagaggaagt agctgaagcg tttgatgaat tggaacggtc
    40141 aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa
    40201 aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa tttggcttga aacatactgg
    40261 aatgatcgat caagggattc atgtcaatat gacagatgaa gcaagtatcg atcatgacgg
    40321 tagtgtactt gattattccc gattgcatga tatgacgatc caagcatggt caccatacca
    40381 gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac
    40441 attagaagaa ctagcagcta aatatcaaac gacaccaact ggcttggctt ctgcttggat
    40501 tttacgccat ccagccaata tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa
    40561 ggaaatcgct caagcttcag aaattaatat gtctcgggaa gattggtacg aattgtaccg
    40621 tgctgctgga aacattttac cttaaagatt gtcataagca ggtcaaaagg gctataaatt
    40681 aaaaagacag aataacttga aagctgtttt cagcttcgag atattctgtc tttttttatt
    40741 gcaaatggaa agagtatcat tgtacggatt tcgtttatta gttatttttg tttttaagga
    40801 aaagctgtgt ccgatattct tttggagaaa tcccttgttt tttcttaaat aaataactga
    40861 aatagttagg gtcattataa ccaatttcaa aagcaatatc tgaaatacga tagtctgatt
    40921 gctgcaataa tttttttgct aattcgattc tttgttctgt cagataatcg atgaatgttt
    40981 ggttaaacga ttgagaaaaa atcgtactga aatgagaggg acttaatgca acttgttgag
    41041 cgacccaagt taaggacata tctggatcgg taaaattttc tttgataaaa tccaatgcat
    41101 ggttcaaaat tggctgatat ttgttccttg aaggctgtga gggcagagca gttaagaaag
    41161 aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt
    41221 gactggtgga tgtgcaatga accatttctt gcgggatttt cttttctttc aggcagacat
    41281 gctggagtag cttagtcaag acaaataaac gatataatcg tgttcgagtt ggtgtgcctt
    41341 gtggtttcgt caagtgttcg atgtacttag atacttcgtt tttcgataat gaagacaaat
    41401 caagtgagag gtcaaagtaa aagacagaat caactggaag tttcgcttct tgaatcaaat
    41461 cctcatagct gataatttgt tctttaaggg aactggcggt ttcaagtatc atctgcgtga
    41521 tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac aggaccaatg gcgacagaaa
    41581 aattattttt ttgtcccttt tctaattcat ggaccaacgt ttgggctacc tgataactgg
    41641 tctcgatcac attttcttca agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa
    41701 cagcagagaa gatcacttgt tgattctccc taaataaaaa cttcaaataa tcactgagat
    41761 gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc taataatacg gtcatttttt
    41821 tccctgctaa agagcgattg aaagattcag caagtttcaa tgtttcactg atcgtaagtg
    41881 agcctttgaa caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca agatcagaag
    41941 aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac tgctttttct attgcttgtt
    42001 tcaattcgct acttttaatc ggtttaagca gataatcatc tgcctgtacc tggattgctc
    42061 ctttcatata atcgaaatcg tcgaatccac taataaatat gagacgagtc caaggaagta
    42121 gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg atatcagtca
    42181 gaatgatgtc gggctgtaag tcgataattg aagacagtgc catctcacca tctccagctt
    42241 cgccgacata agtgattgga taataagaac caaggacagt tatttgttcc cgcaagttat
    42301 tgcgaatgag gtattcgtct tcgatgataa aaactttata catgtttgtt tcctccaatt
    42361 gttttgggag atagagggat catcagtgtg acagttgttc cttctcggta tttgctgtca
    42421 atcgtgatag gtttttgatc atttccgtaa taaagaagga gacgcttgtt tacgttgtaa
    42481 agtccgtagc cgatactgaa atctgaatcg atccctttgg ctaactcttt acgtatttct
    42541 gccagttttt ctgcagggat gccaatcccg ttgtctataa ctttgaattg gatcatctca
    42601 tcaattttgc atgcggtaat acgaataaat cctttgtgac ggacgtactt cgttccatgg
    42661 tatacagcat tttcaatcaa tggctgcaag atcattttta aaatagaaac aggaaaaagt
    42721 gtttctggta tatcgatctt atagtcaagc atatggccgt atcttatttt taagatgact
    42781 agataatcat tgatatggct aatttctttt tcaagaggaa tccagtcttg tcctttgctt
    42841 aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag
    42901 tcttgttcga tcaaagaaat aatggcatcc aaactgttat aaataaaatg gggagtaatc
    42961 tgagcttgca atacacgaac ttcactttgt gctaaatggt attgtttcaa agcgttctcc
    43021 cgtaaagact ggtttagatc ttccgccatt ttattcatgc tagcggtaag tgtttgtaat
    43081 tccagtgttt ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac
    43141 atctcgatca gctgattgat cggttcttgg acctttctct ctaaaaagcg acgattcttt
    43201 ttcgcataga aaaaaatggt actaagaact ccaagttcca ctattgttaa tagaatgagc
    43261 gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga tctccacacg aacaaaatct
    43321 tgtaagatat ctgacagcag ttgagtgaca gaatctacct gattgatgat ctcttcattg
    43381 gtatccataa aatcttttgc caagatatta ttgatcagct cgttttgata atcttccagt
    43441 gtatcgatcg tacgtagcga tacatctaac gtactaattt ctttaagtgt atttgtatta
    43501 tttttgatct ctcgaatatc attcctaagt tcttctagga tattttcctc attgaactca
    43561 tctacggtta cttggccaga aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc
    43621 ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg tctgttgata aatcaaaagc
    43681 tgttgcgaat ataacagtat gctcagtagc aaagggataa cagcaaatag aagcatggct
    43741 ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat acttctccag
    43801 tcaataattt ctggttggga tgttagctat attctgctgg gaaaagacag tctcggaaat
    43861 aaaaatatct ttaggtaatg tttttcctgt ttgaatattt ccaaaataac gagtaacggt
    43921 attggctaca taccagccga catcaggttt acactcgact acacaattga cgattcctga
    43981 gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt acaatgatca aatcttctcc
    44041 aggtttgatt gatgtttctt ttatcgcagc taaagcaccg tgagtcattt catcactatg
    44101 gctgtataat acatcgattt cggataaatc atttgtttct aaatatcgtt tcatctggtc
    44161 ttttcctttc atccgaataa aatcaccgac aattcggtga acaatagtca tcggaggttt
    44221 atggtcgatg gttccatctt tttttagggt tggtttacga ttgattgatt ccaaaaatcc
    44281 ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca gcaagttcta ggattctgat
    44341 ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat aaacccgccc gatttccttc
    44401 tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg
    44461 tctgtcgaca agtatgacag ggatccctgc tcgctttgct tcttttaata cagagtccca
    44521 cccggattct tgcaaaggag taaaaatgat catatccact ttataggcaa tgaacgtccg
    44581 tatatcttgg atttgccgtt cctgattcat gtagccattt cggtagagaa cttggtaatc
    44641 ttctttttca agcgcagtga taattgattc agtatgcttt ttccgccagc tactctccgt
    44701 tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat taggtgtaca
    44761 ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa aataaaacac gtttcacttt
    44821 aaatcactcc atctggattt gttagtacta tcttatcaga taaaagggaa aatcaaagtc
    44881 aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca aaaaaagtac
    44941 acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt ctttattagc
    45001 tatactcggt gtatcaattg aaatggggga taaaaaatga aaaacaaatt tagaaagatt
    45061 gttgggacta tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg caattcagga
    45121 tctgcagatg atagtacggg gtatgtagga atcgctatgc cgaccaaatc agcagaaaga
    45181 tggatcgctg atgggaacaa catggtctcg gaattagaaa aactgggtta taaaacagat
    45241 cttcaatatg gagaagataa agtagaaaat caagtagcac aaatagaaaa tatgattaca
    45301 aaaggggtag atacacttgt gattgcttct atcgatggtt cggctttaac tgatgtgtta
    45361 gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg atcgtttact gatgaattca
    45421 gaaaatgtcg attattacgc gacttttgat aattttggag taggtgtatc gcaagcagct
    45481 tatattgaag agcacttagg attgaaagaa ggaaaaggac cattcacgat tgaattattt
    45541 ggtggctcgc ctgacgacaa caacgcacta atcaattaca acggagtgat gtctgtttta
    45601 caaccatata tggacaacgg tcagttggtc gtgccatctg gacagacgag tttcagtcaa
    45661 atcgctacgc ttcgctggga tggttctacg gcgcaagcaa ggatggataa tctgttaagc
    45721 gcaaattata ctgaccaaac attggatgct gttttgtcgc catatgatcc aataagttta
    45781 ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg
    45841 attactggac aagatgcgac agttgctggc gttaaatcga tcattgccgg ggaacaaaca
    45901 caaacgatct ttaaagacac acgtatctta gcaaaaaata cgattgaaat gatcaaagca
    45961 atcagtgatg gagaagaggt tccggtcaat gatactgaaa cttatgataa tggggtgaaa
    46021 actgtcccta cgtatttagc aaatacagta tctgtcgata aagataatta tcaagcagag
    46081 ctgatcgaca ccgattatta taaagaatcc gatctgaaaa attaagagga accaaacaag
    46141 gaactaaaga gatctaagtc agagagagga taagaattat ggcggattgt attttaaaaa
    46201 tggcagatat caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg aatctgatga
    46261 ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga
    46321 tgaatgtctt gagcggcttg tatccatatg gctcctatga cggggaaatc ttttatgatg
    46381 gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc gtcatcattc
    46441 atcaagaatt agcactaagt ccttatcttt caataaaaga gaatattttt ctcggaaatg
    46501 aacaagcaga aagaggcgtg attgattggg aactgacaga aaagaaaaca tatgaactgc
    46561 tgaataaagt cggtttgtct atcgacccga atcttctagt atcccagatt ggcgtagggc
    46621 aacagcaact agttgaaata gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg
    46681 atgagcctac cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag
    46741 agttgaaaga gcaaggtgta acctccatca ttatttcaca taagctgaat gaaatcgtgg
    46801 atatcgcaga tcgaatcacc attttacggg atggacagac gattgagaca ctagaaaatg
    46861 aaatgatcag cgaagagcat attattcgcg ggatggtggg cagagattta gttgatcgtt
    46921 atcctgaaag aaaaccggat attggagaag tttattttaa agtagaagac tggacggtct
    46981 atcaccctct tgatcacgag cgaatcataa acgaccacat caacttgcat ttgaaaaaag
    47041 gagagattgt aggaatagct ggactgatgg gagcgggcag aactgaattc gctatgagtc
    47101 tatttggaca ttcttatgga agcaagatat ccggacgtat attaaaggaa ggggcagaga
    47161 tatcagttaa gaccgttcct tcagccattg caaacggact cgcttatgtc tcggaggacc
    47221 gaaaatcttt aggactgaat ctgctgatgg atattcggga aaacgtgacg ctgagcagtt
    47281 tggataaaat aagtcaaaaa ggagtattag ataaagaaaa agaagtaact gaggcagaaa
    47341 atttccggaa aaagatgcgt ataaaaacaa actccatcca tcaaattgtc agtagtctta
    47401 gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa cccgatattc
    47461 tttttttgga tgagccaacc cgtgggatag atgttggggc aaaatatgag atctacacga
    47521 ttatcgaaga aatggcagca aatggcaaag ctgtctgtct tatctcttct gaacttcctg
    47581 aaatattagg gatgtgtgat cgaatctata cgatgaacga aggacgtatt acaggagaag
    47641 tctcgcgaga agatgcagat caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg
    47701 gttaagttgg aacaggcaaa aaatcttacg gataaaaaag agaaaaaact aaatttgaaa
    47761 agtagagcag ctgatctttt tggtcgctac agcatggtga tcattttagc gggactactt
    47821 attgcgtttc aattgatgac ggatggtatt ttctggcgtc cattgaatat aactaacctt
    47881 gttctgcaaa atagccatat attagtacta gctgcaggga tgttattagt tgtgctctta
    47941 ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg
    48001 atgatggtca atcgccaaat gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc
    48061 gggctgatcg gcgcatggca aggtttttgg gtagcttatg cagggatacc ggcgtttatc
    48121 gtgactttag caggattgct gatgtttcgt ggactgacac aagttgttct aggaggccaa
    48181 tctctagcac cttttccagc tgttttccaa aaaatgtcta cgggttattt accagactta
    48241 agcgaaggaa gcagccttca cttcttgaca ttgattttag gaatgatcat cacgatcgtg
    48301 ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa
    48361 tctccaacag ctttcttggt aaaggcggta tttacagcag tcgttgtact aggaataact
    48421 tatatctttg cctcttatca agggtatccg attattttga tcattcttgg tttgatcgtt
    48481 gcagcatatg ggtttttgac aaataaaaca gtagcaggtc gacagattta tgcgacgggc
    48541 ggaaatcgaa aagcggctga actatcaggg atcaaaacaa agcgaatcac gttctgggta
    48601 ttcgtcaaca tgggagtcat ggcagcttta gctggattag ttttagctgc tcgattgaat
    48661 gcggcaacac ctcaagcagg tacatcgatg gaactggatg caatggcggc agtatatttt
    48721 ggtggtgctt cgacttccgg cggcattgga acaatcatgg gtgcgattgt cggtggcttg
    48781 gtcatgggtg tattgaacaa tggcatgtcg attttaggcg tcggggtaga ctggcagcag
    48841 gcaatcaaag gattgatctt gcttttagcc gttgtgctag acttgtataa taagaaaaaa
    48901 cgaacaacgt aggagaacgc cggttattca gaaaaaagac tgtgacaaga catttgtcac
    48961 agtctttttt tcgctaaaag cgaacattca ttgtttcgtt tgtatagact aaaaataata
    49021 tatgtttttt taccattcgt ataaattctc tttttgctct tgattttttt gcttaattga
    49081 gttaaattac atagggcgct aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc
    49141 tggatgtgaa catcaggagg ataatgtaat gaaagtattt gattacgaag atattcaatt
    49201 aattcctaat aaatgtatcg taaatagccg ttctgaatgt gatacaacgg tcacattagg
    49261 taaacataca ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta ttgatgaaac
    49321 aatcgcagaa ttcttagcgg aaaatggtta tttctatatc atgcatcggt ttgatgaagc
    49381 agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt
    49441 tggcgtcaag aaagaggaat attcctttat agagaagttg gcagaagaat cgctaaatcc
    49501 tgattatatc actatcgaca tcgcgcatgg acatgctaac tcagttattg atatgattca
    49561 gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga
    49621 agccgtccgt gaattggaaa atgcgggcgc agatgcaaca aaagtcggga ttggtccagg
    49681 aaaagtatgt attacaaaaa tcaagacagg ctttgggact ggaggctggc aactggcagc
    49741 tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg ggatcagaac
    49801 acatggcgat attgcaaaat ctgtccgttt tggtgcgaca atggtcatga ttggttcatt
    49861 gtttgccggt cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa
    49921 agaatacttt ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa
    49981 gaaaatctgg atccaacaca aaggatcttt gaaagataca ctagtggaaa tgcaacaaga
    50041 ccttcaatca tctatttctt atgctggagg aagagatctg gaagccattc gaaaagttga
    50101 ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa taaataaaat
    50161 attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata agaacagcca aacaaaaata
    50221 gttctttata tgttttgttt ggctggaatt attatcgcag atacaagctt ttgaacatca
    50281 ttattcagaa taagggactg tgataagatt tttgtcacag tcccttatgt tttgcaaaaa
    50341 aagtttttct tttttctcgt aaattcggta aaatcaaaag aaaaaataga caatagaaaa
    50401 gagaagctta tgtgggaaaa agtagagaaa aaacaaggac cgatttatcg tcaaattatg
    50461 gaacagatta tgagagaaat cgaaaacgga aggatcaatc ctggaagtca acttgaatca
    50521 gaacgaaaac tagggcaact ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa
    50581 gagcttaggg agcttggggt actaacgagc aaacgtggca gcggatggtt tgttaatcag
    50641 actgaatggg ggaaattcgc tgttccaaga atcgattggc ggcaaatgat ctctcctcgt
    50701 tatgagcaga cggactggta tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca
    50761 tcgtttcttg atttatacgc tagcgagatg ccagatgagc ttttgccgaa ttttgagttt
    50821 ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag aaatggatct tttaggctat
    50881 actccgttac gtaacaaaat caaacggtat ttggaaagta aatttgaatt ggcattttcc
    50941 cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa tctttttgat tttacaaacg
    51001 attctttcag ctggagaggg gatagctgta gagtcacctt ctttttttta ccgattacct
    51061 cttttcaaag cttcaggaac gcgtttattt gggataccga tggatgaaga agggatcgac
    51121 ttagaaaaac tgaaagaatc gatccataaa aataaattga aagcagtgct tatcaacccg
    51181 aattttcaaa accctacagg aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa
    51241 ttgtgtcgac agtatcggct tccaattatt gaagacgatg tatttacgga tctttcattt
    51301 ctaaaccaag aagaagtcct tcctatacga acgattgatc cggataatgt gttatacgtc
    51361 ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg gttgggtgat aggacctgag
    51421 acttttatct cgcaactagc tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt
    51481 acacaaatca cagtggctaa tgtatttaat gaagcaattg atttgaaaat gcaagatgta
    51541 agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt ggggagattc acaatcgttt
    51601 ttcaagcttc attcaatttt aggtggttac tatgcctgga ttacttggga tgggaaaaaa
    51661 ctagaccccg aactagcaga aaaagtagta gatatgggtc ttggaattgc accaagtcta
    51721 ctttttgggc aagatataaa tggaatcaga atcaattaca gtaggctgga tgaaaaccaa
    51781 ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt ggttaacaga ataatttcac
    51841 aaagtaaata gattaaagaa tataacagct tggatttaaa tttcaagctg tttctttttt
    51901 ttcggaaaaa gaatacgata taaagaaact aagaaaaaac cattatttgt acgtccgttc
    51961 acgatgtgta aataaaataa atgtttgcaa caaaactgta taacaccaac gtttttaaat
    52021 agaaacagat ggttgtaagt ttgattagaa aatagaaaaa atgcagatga aaaataaaac
    52081 gctttcctta ttgtgatttt attcactaat gctgttaata tgcggattgg cataatttcc
    52141 tttcatcgtg aaaatttgtg cttttagctt gtatttctca taaatccatg ataatttatt
    52201 aatgaagacg gttaaatagt gatacagttt tattcactgt ttcaatcgca gaatgaagag
    52261 attttcttat agatgagaga ggaatgtgtg acaaatggca aacaaaaaag caccgatcga
    52321 cttccaagca ctgctagatg aagtcaatgc agatttccca gtatatcaag cacttgatca
    52381 agatggaaac gtagttaaca aagatttagt acctgattta tccgatgatg aactcgttga
    52441 attaatgact catatggtat ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg
    52501 ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc agctggctag
    52561 tgcttacgca tttgataaag aagatgttct tcttccaggt tatcgggatg tacctcaact
    52621 agtaaaacat ggtttacctt tatctcaagc ttttttatgg tctcgaggac atgctgcagg
    52681 taattcttat ccggaagaat tgaaagctct accaccacaa attatcatcg gtgcacaata
    52741 tgtacaagca gccggtgttg cattaggttt gaagaaacga aacaagaaaa atgtcgtctt
    52801 cacttatact ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc
    52861 tggcgcttac catgcaaatg ccgtatttta tatccaaaac aacgggtatg cgatctctac
    52921 acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg cagctgggat
    52981 tcctggtatt caagtcgatg gaatggatcc gcttgcagta tatactgtat ctaaaatggc
    53041 acgggaatgg gcgatcagtg gaaatggacc agtcctaatc gaaacactga cttatcgtta
    53101 tggaccacat acactatctg gtgacgatcc aacccgctac cgtacaaaag acacagatga
    53161 cgattggcag aaaaaagatc cattgatccg cttccgtaaa tatttgacgg aaaaaggact
    53221 ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc
    53281 tattgctgaa gccgacaaag taccaaaaca aaaggtttca gatttcttga aaaacatgtt
    53341 cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa
    53401 ataatcatgg cacaaaaaac aatgatccaa gcaatcacag atgccttagc acttgaacta
    53461 gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg aggagttttc
    53521 cgggcaactg aaggattgca ggaaaagttc ggagaagacc gagtattcga tactccttta
    53581 gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt
    53641 cctgaaatcc aattctttgg ttttattttc gaagcaatgg acgaagtagt agctcaaatg
    53701 gctcgtacaa gatatcgaat gagcggcact agaaatctac ccatcacaat tcgttcgcca
    53761 tttggtggcg gtgttcatac accagaattg cattcggata atttagaagg tttgatcgct
    53821 cagtctcctg gtattcgagt agtgattcct tccaaccctt atgatgcaaa aggactattg
    53881 atttcttcta ttagaagtaa cgatccggtt gttttcctag aacacatgaa actatatcgc
    53941 tcattccgag aagaagtgcc ggatgaagct tacgaagttc ctttggataa agcagcagtt
    54001 actcgtgagg gaacagatgt atctattatc acatacggag caatggtgcg tgaagcaatc
    54061 aaagcagcag acaatcttgc aaaagaaaat atatctgttg aaatcattga tcttcgtaca
    54121 gttgcgccat tagatgtaga aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt
    54181 gttgtccaag aagcacaacg acaagcgggc gttgccgcgc aagtagtttc tgagatctca
    54241 gaacgcgcta tcctttcatt agaagcccca atcggacgag tttctgcacc tgatacagtc
    54301 ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa
    54361 gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa
    54421 tttaaatt
//
LOCUS       DAZKAS010000004        51075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000004 DAZKAS010000000
VERSION     DAZKAS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.004"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..1124)
                     /locus_tag="ACG8H7_000167"
     CDS             complement(105..1124)
                     /locus_tag="ACG8H7_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8982308.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1146..2153)
                     /locus_tag="ACG8H7_000168"
     CDS             complement(1146..2153)
                     /locus_tag="ACG8H7_000168"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP8982309.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="ACG8H7_000169"
     CDS             complement(2146..2811)
                     /gene="pgmB"
                     /locus_tag="ACG8H7_000169"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP8982310.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(2804..5098)
                     /locus_tag="ACG8H7_000170"
     CDS             complement(2804..5098)
                     /locus_tag="ACG8H7_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP8982311.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5358..7523
                     /locus_tag="ACG8H7_000171"
     CDS             5358..7523
                     /locus_tag="ACG8H7_000171"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFP8982312.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7827..8660
                     /locus_tag="ACG8H7_000172"
     CDS             7827..8660
                     /locus_tag="ACG8H7_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP8982313.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8762..10462)
                     /locus_tag="ACG8H7_000173"
     CDS             complement(8762..10462)
                     /locus_tag="ACG8H7_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982314.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10969..12129
                     /locus_tag="ACG8H7_000174"
     CDS             10969..12129
                     /locus_tag="ACG8H7_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8982315.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12145..12588
                     /locus_tag="ACG8H7_000175"
     CDS             12145..12588
                     /locus_tag="ACG8H7_000175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8982316.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13071..13601)
                     /locus_tag="ACG8H7_000176"
     CDS             complement(13071..13601)
                     /locus_tag="ACG8H7_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFP8982317.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13706..14257
                     /locus_tag="ACG8H7_000177"
     CDS             13706..14257
                     /locus_tag="ACG8H7_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8982318.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14410..14883)
                     /locus_tag="ACG8H7_000178"
     CDS             complement(14410..14883)
                     /locus_tag="ACG8H7_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8982319.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15021..16493
                     /locus_tag="ACG8H7_000179"
     CDS             15021..16493
                     /locus_tag="ACG8H7_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP8982320.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16490..17200
                     /locus_tag="ACG8H7_000180"
     CDS             16490..17200
                     /locus_tag="ACG8H7_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFP8982321.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17552..17836
                     /locus_tag="ACG8H7_000181"
     CDS             17552..17836
                     /locus_tag="ACG8H7_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFP8982322.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18117..18536)
                     /locus_tag="ACG8H7_000182"
     CDS             complement(18117..18536)
                     /locus_tag="ACG8H7_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFP8982323.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(18538..19122)
                     /locus_tag="ACG8H7_000183"
     CDS             complement(18538..19122)
                     /locus_tag="ACG8H7_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFP8982324.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(19169..20644)
                     /locus_tag="ACG8H7_000184"
     CDS             complement(19169..20644)
                     /locus_tag="ACG8H7_000184"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFP8982325.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20825..21136
                     /locus_tag="ACG8H7_000185"
     CDS             20825..21136
                     /locus_tag="ACG8H7_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982326.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21251..23587)
                     /locus_tag="ACG8H7_000186"
     CDS             complement(21251..23587)
                     /locus_tag="ACG8H7_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP8982327.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23729..24397
                     /locus_tag="ACG8H7_000187"
     CDS             23729..24397
                     /locus_tag="ACG8H7_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8982328.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24400..25443
                     /locus_tag="ACG8H7_000188"
     CDS             24400..25443
                     /locus_tag="ACG8H7_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8982329.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25680..26561)
                     /locus_tag="ACG8H7_000189"
     CDS             complement(25680..26561)
                     /locus_tag="ACG8H7_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8982330.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26720..27448
                     /locus_tag="ACG8H7_000190"
     CDS             26720..27448
                     /locus_tag="ACG8H7_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP8982331.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27604..27921
                     /locus_tag="ACG8H7_000191"
     CDS             27604..27921
                     /locus_tag="ACG8H7_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8982332.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27914..28522
                     /locus_tag="ACG8H7_000192"
     CDS             27914..28522
                     /locus_tag="ACG8H7_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFP8982333.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28577..29368
                     /locus_tag="ACG8H7_000193"
     CDS             28577..29368
                     /locus_tag="ACG8H7_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFP8982334.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29506..30018
                     /locus_tag="ACG8H7_000194"
     CDS             29506..30018
                     /locus_tag="ACG8H7_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFP8982335.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30044..30916
                     /locus_tag="ACG8H7_000195"
     CDS             30044..30916
                     /locus_tag="ACG8H7_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP8982336.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31092..31454
                     /locus_tag="ACG8H7_000196"
     CDS             31092..31454
                     /locus_tag="ACG8H7_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP8982337.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31463..32215
                     /locus_tag="ACG8H7_000197"
     CDS             31463..32215
                     /locus_tag="ACG8H7_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982338.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32229..34031
                     /locus_tag="ACG8H7_000198"
     CDS             32229..34031
                     /locus_tag="ACG8H7_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982339.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34308..35348
                     /locus_tag="ACG8H7_000199"
     CDS             34308..35348
                     /locus_tag="ACG8H7_000199"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP8982340.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(35400..37544)
                     /locus_tag="ACG8H7_000200"
     CDS             complement(35400..37544)
                     /locus_tag="ACG8H7_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8982341.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37901..39274)
                     /locus_tag="ACG8H7_000201"
     CDS             complement(37901..39274)
                     /locus_tag="ACG8H7_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8982342.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39274..39669)
                     /gene="rbsD"
                     /locus_tag="ACG8H7_000202"
     CDS             complement(39274..39669)
                     /gene="rbsD"
                     /locus_tag="ACG8H7_000202"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP8982343.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39697..40614)
                     /gene="rbsK"
                     /locus_tag="ACG8H7_000203"
     CDS             complement(39697..40614)
                     /gene="rbsK"
                     /locus_tag="ACG8H7_000203"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP8982344.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40722..41735)
                     /locus_tag="ACG8H7_000204"
     CDS             complement(40722..41735)
                     /locus_tag="ACG8H7_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP8982345.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41816..42415)
                     /locus_tag="ACG8H7_000205"
     CDS             complement(41816..42415)
                     /locus_tag="ACG8H7_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP8982346.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            42601..43302
                     /locus_tag="ACG8H7_000206"
     CDS             42601..43302
                     /locus_tag="ACG8H7_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982347.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43312..46023
                     /locus_tag="ACG8H7_000207"
     CDS             43312..46023
                     /locus_tag="ACG8H7_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFP8982348.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            46420..48048
                     /gene="treC"
                     /locus_tag="ACG8H7_000208"
     CDS             46420..48048
                     /gene="treC"
                     /locus_tag="ACG8H7_000208"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP8982349.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(48108..49289)
                     /locus_tag="ACG8H7_000209"
     CDS             complement(48108..49289)
                     /locus_tag="ACG8H7_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8982350.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            49541..51064
                     /locus_tag="ACG8H7_000210"
     CDS             49541..51064
                     /locus_tag="ACG8H7_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFP8982351.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        16083 a         9170 c        10426 g        15396 t
ORIGIN      
        1 cacgctcttt tttacagata aacaatgtgt tagaagtagt atcttctttt acataaaaag
       61 ctgaccatag gataaatggt cagcgtattt agtgagtcag ctatttagta tgggttttct
      121 aaaggaacga ctgtctctct tttgatcaag cgatgaggga tgaccaaacg tacgccactg
      181 ctcaattctt catggatcaa ttcgcgcagc ttttgcatag cgacacgtcc taattcggag
      241 atatcgatat cgatacttgt aaggtaaggg tgtgttaatg ttgagaagat cgagttgttg
      301 aagctaatga cggagagtgt ctctggaatg acatagccgt acatttgcgc caactgcatc
      361 gtgcgaaggg cgaagatatc gtcaatgacc accaatgctg tggcttcgtt ttctttcaac
      421 gttttctcaa aggacagata atcctccgga tgctctagta ctacactgga taacgcagga
      481 agaccagcca tcatcattgc tttttgataa ccgaaatatc gttcgaaata caaactttca
      541 tgagtcgtgt ttgtcacaaa caggatattc ttatgtccat tctggatcaa aaattctgtt
      601 gcttgtttac ctagaagctg gttatcatta tctacataaa cgatttcgtt ctcatgatga
      661 taaggctgtc cgatcaaagt gaaagggatg tggttttggt ataaataatc aatgacagga
      721 tcttgactat ctgaatacac tagaataaaa ccatctactt gtttttgtaa atgcatacgc
      781 tggacgttct ctaacagcaa ttcaaaattt ttagcagtag cgattgctgt agtcatatca
      841 taattccgcg cttcttcgtt gattgcttcc atgatctcaa gataaaatgg gttaccgatc
      901 cgctcttttg aatcaagagg gggtaagatt acgccgatgg cactggaaat gcgttttcct
      961 agattgcggg cagtcatgtt aggaacataa cctagttcat ccataatttt tcggactttc
     1021 ttctttgttg tctcggaaat actaggatga tcgttgatga cacgagagac ggtcgaggtg
     1081 gcaacaccgg cttttttcgc cacatctttt acggtaatag ccatgataaa aacacctttc
     1141 tcttgttatt gtgcagagaa ccgataaatc gtctgactga tccgttcttg accaggatgg
     1201 aggttgatcg atccaaattt ggggaaatga acgatatcag ggatttcttg cggttcgatt
     1261 gctaatccgt agtttgaatg catctgtttt ccattgacag agaaatcagc ttcgaatcca
     1321 gtcgtagaaa ataataccat acttttatta gaagttgcta tagtcatttg gcgaccagaa
     1381 tgcttctcga ataattgtaa ggaagttttg gaaagcctag gatgatgcaa aagaaataca
     1441 tcatctagtc catctggata acatgcaagg atctcttgca gtgttgggga ttttctaaag
     1501 tcgattggca gtttttcttt ttttgtccaa tttccagtag gcagtttttc ttgatctaat
     1561 tcaagcacac catctaaaaa gaccgttaac tgatgttcat agatgtctct tgttccattt
     1621 cctgaaaggt tgaagtaggc atggttggtc ggattgcaga tggtttcaac aaggctacta
     1681 gcagaggttg tcatctctaa ttcgtttttt gttaaacggt aagtgatcgt agcagtgatt
     1741 ggtcctggat aggtagaaaa ttcatctttt aagtaaaaga ccacacctat tgatgatgga
     1801 gttttgaaaa cttctacttc ccaaaattgg aaagaccagc ctttcgtgcc accgtggata
     1861 tggtgtgatc cgcagttttt ctccaatcta tgctcacccc aagaagcatc ccggatcctt
     1921 ccggctacag gtccgacagt tgctccaaaa aagcttttgt ctgttagaac atcttcgaaa
     1981 cgatcgtaag acaataaaac attttctgat tttccttctt tgtctggtgc gaagatttgg
     2041 tgcaggcggg caccgtaatt gagaagaacg acttctaagt agtcgttctt caatgtgatt
     2101 ttctttattt cagtctctgg aactggtacg acggagatct ctggattatc catggtcatg
     2161 acacttttct ttcaagtaat cgatcgtaag aaaagtcgta tcagggacaa gcgggatctc
     2221 ctctccaagg tcttcagcac gtcccacgcc aattggttga gcgccacttg agaggatggc
     2281 ttggataccg gcttgcgcgt cttcgatccc gatacattct tctgctttta gtccgacagc
     2341 ttttgctgca aggaggaaga tatctggcgc aggttttccg tttgttgctt ctgccgggtc
     2401 ggcaatagca tcaaagtaag gtgtaagacc cattttttca agtaggaatg gcccgttttt
     2461 acttgcagaa gcaagagcaa tcttgatttt ttcttctttt aaagaatcca gtaaaggcac
     2521 gaccccgggg aagacatctt ttggttcgat cgtctggatc atctctaaat aataatcgtt
     2581 ttttcttttc gctaattctg caaattcttc ctttgaaaaa gagtgttctt ttccgccata
     2641 agctaaaagc aaagagagtg agtcttctcg gctgacgcct tttagctgtt cgttgaatgt
     2701 gtggtcgatc gtaatgccga tttcctgacc aagctttcgc caagcacgat aatggaattc
     2761 agcagtatcg gtaatgacac cgtctaaatc aaacaatacc cctttaaaca tttgcttcct
     2821 cctttcttag cgttatttct agacgatccg ctaaagaata agactgttca tagacgtaaa
     2881 tttctagagg aaaaccttct aataactcga aagtaacttg ttcatctgtg actgagatga
     2941 acaataaacg tccgcgataa ttgatatgga aagaataagc tttccaagtt tttggcagaa
     3001 atggtgcgaa tctcaactga tcatgggcag ttttcatttg agcaaatcct tggacaatcg
     3061 acaaccagct accagtcatg gaagtgatgt gcaagccgtc ttctgtgtcg ttattgtaat
     3121 tatccaaatc caagcgagct gttcgctggt acatttctac agctttgctt tccattccta
     3181 attctgctgc taaaatcgaa tggatacttg gcgaaaggga agactcatgg acagtcattg
     3241 gttcatagaa atcaaagttt ttttgctttt cttcttttgt aaaagtatca ttgaagaaat
     3301 aaatcccctg tagtacatct gcctgcttga tgaagcaaga tctaaggatt ttgtcccaag
     3361 accaatgctg attcagagga agatcgcttg tgggaatatc ggatacaggc atgagatctt
     3421 tatctaagaa tgtgtcatgc tggacaaaga ttcctaattc ttcgtcaact gggaagtaca
     3481 tgttctctgc gatatctttc catttggcta gttcttcttc gctgatagtg atagcagctt
     3541 ctttttgata agttaaataa ttttctcgcg tgtaacgcaa gacccaagct gcgatcgtat
     3601 ttgtatacca attgttattg atattgttct catattcgtt tgggccggtc acaccgtgga
     3661 tcatgtattt atttgctcgt tttgaaaaat ggacacggtc tgcccagaag cgtgcgattt
     3721 ctgctaaaac ttccagtcct tcatttttca aatattccgt atcgccggtg taattcgtat
     3781 aattgtagat tgcataggca atcgcgccat ttcggtgaat ctcctcgaat gtgatttccc
     3841 actcgttatg gcattcgaca ccagtgaagg tgaccatagg ataaagcgcg ccttttaatc
     3901 cttgttgctg agcgttgtgg attgcttgcg gcagttgatt atgccgatat ttcagcagat
     3961 tcttagttac ttcaggtttg gctaaagcaa gataaagagg gacagcatac gcttctgtat
     4021 cccagtaagt ggctccgccg tatttttctc ctgtgaatcc tttggggccg atattcaatc
     4081 gttcatcttc gccgtagtaa gtagagaata gttggaacaa attgaatcga ataccttgtt
     4141 gtgcttcttc gtctccttcg atgaccacat cggcaagctt ccatcgttct gcccaagccg
     4201 ctgtttgctc ttcttttgct tggatataat tgtttgtctg gttcgttaat tctgagacag
     4261 ctttttctgc ttgctgcgct tctggaatat ctctactagt aagaacgata atttcttttt
     4321 ccactgacca tgtctcacct tgagataatt ctgcagacaa gcaaccatgt aatgctaaag
     4381 tatcttcgtg atagtcaggg atgactgatt ttccattaac gaaatggcgc attgctgcag
     4441 taacagtaaa ttgttcaatc ccaaagttgt ttggaatagt tttagtcgtc aaatagccga
     4501 tttcttctat agttccacga tcaatctcat tccaaaaatg ttcatcatag ttgctgtctt
     4561 cgttttgaac atttccatcc aattttgata cgagtttgac tgttccgcta ccttcaagca
     4621 tttctatagt taaatagata taagctgatt cttttttgac aatgctcaga aaacgttcga
     4681 aggaaatgcg tactttatta gtagaagtag tgattgtgaa tgtacgagat aatacaccat
     4741 ttttcatatc taattcccag taaaagtctt ctggttccaa agaagccaag tcgatcagct
     4801 gatcgttgat cgaaatatct atcgcgataa agttcaaggc gttgatcact tttccaaagt
     4861 attctggata accatttttc caccagccga cacgtgtctt atcgggatac cagacacccg
     4921 ccaaataagt accttgatgg tggtcccctg agtaagtttc ttcaaagttc ccgcgcatcc
     4981 ccatataacc gtttccaata cttgttaaag attcttgcag tcgaagattt tttttatcta
     5041 attgtgtcgt gctgattttc catggatcaa tagcaaatag tcgtttgatc tgtttcattt
     5101 ttctcctcct aatgagctgt tttatacgtt cataataaac gcaaccgatt gcacaagtca
     5161 atagacaaaa gaaaatattt catagaaaaa agcttaaaaa aataaatttt taggagaaaa
     5221 ttatagaagg gaacacacgt attataggtt ttatagaatg gtatagacag aaaaataaaa
     5281 aataaagcat ttacaaaaaa gaaaacgcac gctataatac gtttaacgaa accgattgcg
     5341 taaaggagct aaacggaatg aaaaagatat ttagttttga gttttggcaa aaatttggaa
     5401 aggctttaat ggttgtggta gcagttatgc cggcagctgg gttgatgatc agtataggga
     5461 aatcaattcc cttgatcaat cctgattgga cgccgcttgt cacaacgggc ggagtaattg
     5521 aaaatattgg ttgggcaatt attggtaatc ttcatatttt atttgcttta gcaataggag
     5581 gaagttgggc aaaagaaaga gcaggcggag catttgcagc tgggatctca tttattttaa
     5641 tcaatcggat cactggggct atctttggtg tcacaagtga catgcttgcg aatgaagatg
     5701 catttaccca cactctgttc ggtacgaaga tcatggttaa aggatttttt acaagtgtat
     5761 tggaagcacc agctttgaat atgggagtat tcgtagggat catcgctgga tttgtcgggg
     5821 caatggctta taataagtac tataattata gaaaattgcc ggatgctttg tcatttttca
     5881 atggaaagcg ctttgttccg tttgttgtta tcttatggtc aacaatcgtg gcattgattc
     5941 ttgctgtggt gtggccaaac gtacaggctg gaatcaataa ttttggttta tggattgcag
     6001 aatcacaaga aagcgcaccg attctagcac cgtttttatt tggaacatta gaacgtttac
     6061 tattgccatt tggattgcat catatgctga cgattcctat caactataca cagcttggcg
     6121 gatcgtatga gattttatca ggcgcacagg cggggacaca agtatttggg caagatccgt
     6181 tatggttggc ttgggccact gatttagtga atcttaaagg tgcaggggat atgtcgcaat
     6241 atgagtttgt gttgcataac tggacgccgg ctcgtttcaa agtaggacaa atgattggtt
     6301 cttcaggtat cttgatggga atggcttttg cgatgtatcg taatgtcgat cctgataaga
     6361 aagcaagata taaatcaatg tatttttcag cagcgttggc tgtctttcta acaggtgtaa
     6421 cagaaccatt ggagttcatg tttatgtttg ctgctgtgcc attatatgtc atttattcag
     6481 tgatccaagg ggctgcattt gcgatggcag atatcttgcc attgcgggtc cattcatttg
     6541 ggaatatcga attgctgact cggacaccgt tagctatcaa agcgggtcta ggtggagatc
     6601 ttatcaactt cgtgttgtgt gtgatcgctt ttggtgttgt tacgtatttc ttagcaaatt
     6661 tcttgatcaa aaaattcaac tttgctacac cgggacgtaa cggaaattac gacaatgaca
     6721 gtgaagaaac cgttgtatct aacagcggaa caggaacagc agatcagcaa gtggtccaga
     6781 tcattcattt gctgggagga aaagaaaata tcaaagatgt cgatgcttgt atgactcggt
     6841 tacgcgtaag tgtaaatgat cgagaaaaag tagggactga agaagcctgg aaacgagcag
     6901 gggcgatggg attgatcgtc aaagacaatg gagtccaagc agtttatgga ccaaaagcag
     6961 atgtcttgaa atcagatatc gaagatctgc tgcaatcagg agcagagatt ccagagccag
     7021 aaatggtttc tacaggaaat ggacaagcag gtagtaagca atacttaggt atagaacaag
     7081 aactagtttc cgcagcttct ggagaagtga ttcctttatc tgaagtaaaa gatccggtat
     7141 tctcacaaaa aatgatgggt gacggctttg ctgttattcc aacatcaaga gaagttgttg
     7201 cgccgatcgc tggaaaagtg acaagtattt tccctagcaa acatgcaatt gggctggaaa
     7261 cgaaagatgg tatcgaagtt ttgatccata tgggaatcga tacagtccaa atgaatcagc
     7321 cagcttttga gattgctgtc aaagaaggcc aagaagtagg aacaggaacg aaattagctg
     7381 agatggatct tgatgtgatc caaaacgaag aaaaagatcc aacgatcatg atcgtattta
     7441 caaacgataa agtagaagaa gtagtgatca agcaattagg tacagtgaca gctggtaaag
     7501 tgatcggctc cattaaaatg taaggagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     7561 aagaacagcc aaacaaaaat agctcctcct atttttcgtt tggttgggct tattaccgag
     7621 aagcagcagt ttgaatactg tttagtggag aggtcgtgaa aaagctgccc tgcaccaagt
     7681 gataaaaaca gccaaacaaa aaatagctcc tggtccattg agcttattac tgaggagtta
     7741 gcaggagaac actatttgct ggagaggttg tgacaaaaat tttgtcacaa cccttttgtt
     7801 tcgaaatgaa gacaaggaga aaaccaatga aattcatgac actcaacacg catagctggc
     7861 ttgagccaga accagagaaa aaattgcaag aattagcaga taagatcctg ttagaagatt
     7921 atgaagtgat tgctttacaa gaaatcaacc aattactgga atctgaagaa gtagaacctg
     7981 cgaagctaat gaagttctgt caggtaaaga atcaagtacc tatccgtaaa gataattttg
     8041 cctaccgttt agttcagctt ttaaaggaac acggaaaaga gtatttttgg agttgggaaa
     8101 tgagccacat cggttatgat aaatatgaag aaggaaatgc actgcttacg aaaaaaacat
     8161 tggaaagtca ggtccttacc gtctcccaat ctcaaaagaa agaagattat cagacacgca
     8221 agatcttgat tgggaaaaca aagatagatg atcaggacat ttttgtctcg agctgccact
     8281 tttcttggtg gacagataaa aaaagtggct tttattttga gtggaaaaac ttggaaaact
     8341 actttttgga aactcgcgta ccactttttt tcctaggtga tttcaataat ccagtagact
     8401 ctcaaggtta ttatactgta agagaaagtt gccttcttct tcaagattcg tatgttgtcg
     8461 cgaatgaaaa aggaaaagca gctactgtgg aaaagaaaat agatggttgg gaacaaaata
     8521 cagaaaaatt gcgtatagat tttattttcg tccctgaagg aatgcaggtt aaaaaatatc
     8581 aacggatttt tgatgggatc gatagtccaa tcatcagtga tcactacggg gtggaaatcg
     8641 aattagatgt gaacgaataa aaaatataac atataacata aaaaaactga ttaaaataaa
     8701 actctccaaa cgattatacc gtttggagag ttttttgatc ggatgtattt atcccttttg
     8761 cctaagcacg taaaagcttg tatctatgat aaataaataa acatagcaag gtaaaccagc
     8821 caatcaaagt aagagaaaga atgaaataga aatagattgg cgtttgataa tgaagcgtga
     8881 gctcgttgat tccttttttc tgcttcacga ttggggtccc gatatctgtt acttcataat
     8941 cagtcaatgt ttttccattt tgctgtagca cagtccgatc gtagacgata acagggatat
     9001 gaacagtttt gtccccagtg tttttccact tgattgtcaa aagaccgcct ggatgtgttt
     9061 ttataaaagt tgtttcgttt tctagtattt cttcgttata ccggtcgtat ttattgtctt
     9121 ttgtctcctt atagattggc agatagtcag gtgtggtttt ttggaaaacg aacaagctgg
     9181 tagacatatc tttgctgaag aatgttttcc ttgcttcttc cggcgtatcg aatagataag
     9241 tatgtcgact gacaaatgtt tcattttccc acttttccag atgctggtat aagttctgga
     9301 tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa aagacggaac cacttttgtt
     9361 cagaccactg gttcagtgaa agggcaaggt aaagtagaag caatagcgta aatggcacga
     9421 aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac atttttttcg atcaatgtcg
     9481 tccatggaat caagctagta gaaagaaaaa agaaaacaag acttgtgtac aacacgattt
     9541 tttggaataa ggtgttcttt tgttgaaaga aaataaaaag caaaaaacct gataatatca
     9601 atataggaaa ggccttaggg taatggagcc aataactgcc atccagatct atcgcctttt
     9661 gactcatttc tctgttaaca aagggaggaa gaatcttatt cccactgtaa acaatagcta
     9721 atcctgccca aatatttgct gtcagaacaa aataaagcaa gaccgactta atcagttgaa
     9781 gcagtgcttt tcctttttca gtctggttaa aaaagtaata aagataaaaa gggatataca
     9841 tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt cataagtccc actgagacag
     9901 cgactttgat aacagggaac tttttatgaa ggacgaaatc agcaataggg atcatacata
     9961 aaggataaaa acaagtcccc cagctagtga atccttggtt gatagaccag tattgaatag
    10021 aatacgtact catgaataaa acggctactg gtacacttac ccaatttcga acattgacat
    10081 atctcagtaa aatcagcata gaaaatcctg aaataagata aagaatgaaa tttgaaagaa
    10141 tctgataatg aaaccagcta cctgcaagca acacgatcaa tccttgtaaa taagcgaaaa
    10201 atgggccgta aacggcattc acgatcctgc cactttgctg gaatccataa agggaaggaa
    10261 aataatggaa atccccatat ttaatttgtt gcgcggcatc atagaaccga ttgtaatgga
    10321 atggggcatc agcacccaaa attagtttat gtgtgaataa atgaggcatg agaaatgcca
    10381 aagcaatgaa aaaaattgct aaaaatgggc catatttttt caattgtcga actcctttgc
    10441 aaactttaga taaattcatc atatatccta tgcgagaggt aggcaagaaa ttagaaaatt
    10501 catgaaaata tttatgaatt ttacaagaaa aacataagtg aaagttctaa ggagagagga
    10561 tgcgacataa gttttgttgc atctccttgt tttgaaacag aatttttctt aatactcgaa
    10621 acaaagtagc tttttcatga cctctcggta aacggtgttc aatcagctgc ttctcggtaa
    10681 taagctcact agcctagaaa atatgagaag ctgttttctg actagcgctc ttattactca
    10741 aagcataacg cagttttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg
    10801 gtaataagcc caacccaaca aaaaatatga gtagctattt ttttgggctg tacttattac
    10861 ttgatgcagg acagcttttt cacaacctca tcggctttca ttttctattt ttttcctgaa
    10921 cccatataat aaaagaaaga acaaaaaaac ttgcaaggga ggacacatgt gaagcagagt
    10981 aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt tattaatatg tatcgtcgta
    11041 gggttactga tcgctttata tccattttat gtggattctt taaatagttt tattgatcaa
    11101 aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa ttgaagtgca gcgaaagaaa
    11161 atggaggaga aaaaacaacg attaactgac caaggattca atcctggtac tgatcctttc
    11221 aatgaaaaga acagaaatga aagtacaaca tccagccaat tagaggaatg gctgatcgga
    11281 agtatcaata tacctaagat ccaattaaat atttcacttt atgatcggct caatggtagg
    11341 atccttgaaa atggagcagg cgtcttgcaa ggaacctctt tcccgcttgg tggaaattcg
    11401 acgcattcag tgatctctgc tcacagcggt ttacctaatc gacggttatt taccgaatta
    11461 gatcgtttag aaaacggaga cacgtttatt ttgactgttt tgggggagaa attggcttat
    11521 caagttaaaa atatacaagt ggtactgcca gatgacacgt ctgttctaac gatttcagaa
    11581 ggagaagact tagttacctt gcttacatgt acgccgtata tgatcaacac gcatcgtttg
    11641 ctagtgacag gacatcggat tccttataac gaatcggtaa aaaaagaaga aggaaaagga
    11701 aatcaggaaa gaaccatgcg gcaatggttt attcttgcag gaacgataat agctgtagtc
    11761 atactgttag tgtttatcgg acgactaatc tatcagtata gattaagtaa aaaagtgctt
    11821 gatttttcat ttattattag tgattcgtca gggaaaccgg taaatggtag ttcattcatt
    11881 ttgaaacata agaaaaaaac acttactaga aatggtgtgc cgttttcagt acaaagcgac
    11941 catcatggga aagtcaagct ggatcaattg cctggtggaa cttatcgaat cgtttcagct
    12001 gatgatccta aagttgctgt ttcttttggt attagaaaag taaaacaaga gaagatatac
    12061 ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa atggttttta ttttaaaatc
    12121 aatgaataag taaggagaga aaaaatgatc cgcacaacaa caaaattgtc tgccaaagaa
    12181 ctggaatctg ttctatcgat ttggctgcat gctaacttgg attctcatga attcattcca
    12241 tcaacttatt gggagaatca agtagaatct gttaggaaag ctttaccaga tgcagtgatt
    12301 tacttagcag aaatagatca agaaattatt ggttttgctg gggtgattga tcagtttatc
    12361 gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa tcggtacgca actaatgaaa
    12421 aaaataaaag aaaccaataa aacactgact ttgacagtct atcaaaaaaa tcaatcagct
    12481 attcatttct atctttcaca aggttttaca attcaaacac agcaaaaaga tgaagcagta
    12541 aatgaggagg aacttgtgat gaaatggaat caaaacgctg atttttgatg aacagaataa
    12601 cgaaataatg ctcccagcct aattttttgc tgggagcatt atttcatttt gaagtcacta
    12661 atttaactgt gtgaatatgc agttgttaca atctttgata aacggtgttc aatcagctga
    12721 ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tatttttcca tttgtttctc
    12781 taatactcag gtcaaaacgc tgatttcaca acctcttgat aaacggtgtt caaacggctg
    12841 cttctcggta ataagcccac tagcctagaa aatatgaaaa gctattttct aactagcgtt
    12901 cttattactc aaagcataac gctgatttca caacctcttg ataaacggtg ttcaatcagc
    12961 tgaccctcgg tattagctca aaaaacggca aaacatgagg aactattttt ccgtttcttt
    13021 ctctaatact caggtcagaa cgctgatttc acaacctctc tttcacaacc ttattttact
    13081 cgtttataat tttcatcgaa gtattttccg ctacggatat catctggcat tccatcgtat
    13141 ggttcttcgc taataacatc ttcattgtct atacctgcat cacccatata agtgatcgta
    13201 gcaaattcag gaacaagtaa ttttggataa gtttcatatt tttcacgagc tgcttccatt
    13261 tctgcgatat gttcgttttg gaaagtaaca gtgatttctg gatgttcttc aacccattta
    13321 gggaaaaaga tcattccgtg aagtttcttt tcatttggca taaagtccaa tgccacgtct
    13381 gtccctgttg gctctgtcca agtgattttg aatacgccgt ccgtcaattt aacgatatca
    13441 gcttcttggt cttttaccca acggcctttg accattccgc catggatacg gtaatcaaca
    13501 gtatggtcat ttttggcata ccattcatat tcccaaccat tgtcatacgt atagataaag
    13561 tgagttccaa taaaatcttc caattcttta aattctttca tgtttaagca tctccttata
    13621 aattaaaaca tgtaataaac tacatgtttt cgattactca tttatgatag aatggattta
    13681 aagatttgtc aaggagggaa aactgatggc aagaaaaaat acacttcgat atattttgtt
    13741 agggttatta agtaagaaga agatgacagg atatgaattg aatcaatcgt tcaaaaatga
    13801 aataggagaa ttttggcaag caaaacatag tcagatttat cctgaacttg caaaaatgga
    13861 agagcaagga atcattcagc atcaagtaga gatcacaggt gaaaaattgg aaaagaaagt
    13921 ttatgaatta acagaagaag ggaaggaaca attggcagag tggattcata caccaaccaa
    13981 tgaactaccg gttaatcgag acgaatttgt tctgaaattg tatttcgtca aagacgtgaa
    14041 tgatccggca ttagtagaaa ttattcagca gcagcgtgac cttcatgaag aaaaacgact
    14101 tcatctatta gagcgaaaaa aaacgatctt tcctactgaa aaagaaaaac atgaaaatta
    14161 cggacactac ctgattttag cgcatgcgat caatcgggaa acagaatatg caaattggct
    14221 aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa aatatccgaa cagtgatgga
    14281 gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg gatattctta tgttcaaaca
    14341 ggcctttttt caagtattta tgtagcatct acttattaaa aagcttggta cttaggagat
    14401 tagtggcgtt tagagttctt ttaacaactg ttcgtagtca gctttttcat cgacaggaag
    14461 gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg atgtacatct tagcattctc
    14521 ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg tcgatcaatt cgatatcttt
    14581 gattgctttt gaaaaaactt tacggacatg gtgcattttc ttttggcgga tttctgcctt
    14641 ttctgcagct tttgacaaag taaatccgtc atctaaatat tttttgatca gttctacttt
    14701 tatgactgta ggtaaacgat attttctagg acaattcgca tcagcttcga tcgaacgaat
    14761 gaatcccttt tgttcccaat accttaattg acgtggagac acacctacga tatcacttaa
    14821 ttcactgatg ccaacaacca attgatcatt ttccaacaaa cgtttaaggg aagccccaac
    14881 catttttgtc actccttttg tcctcttgct tcccttaatt atatccgtat taacttatgt
    14941 gtcaagtagg ttgacaaata gcgaatttag gactaaagta ttctagtagt ttcagaaaaa
    15001 attcagaaag aaggagaaat atgagtaaca accaacccgt agatatttac ggaaaaccat
    15061 ataatcggac acttttagtt gttgtcttat taattggtac attttgtacc gttttgaatc
    15121 agaccttatt gacgacagct tttcctacat tgatgaaagc tttcgatatt tcagcgtctg
    15181 atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg gatcatgatc ccaataacag
    15241 catggctgat caataaattc agttcaagaa atttatatct ttctgccatg acgatctttt
    15301 tgatcggaac gattacttgc tttacagcac ctaattttag tacattacta atcggtcgct
    15361 tgatccaagc ttgcggcgtg ggagtatcga tgccattatt gcaaaatatc atgctatcta
    15421 ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc agggatcgtt attggacttg
    15481 ctcctgcttt aggccctaca ttatctggtt atatcattga taactaccat tggcgtgact
    15541 tgtttggtat ggtactgcca atcgttgttc ttgtcttggt tttagcattc ttcctaatga
    15601 aaagtgttat tccattgtca aatcctagca ttgatatttt atccgctatt ttatcaacga
    15661 ttggattcgg tagtttactt tacggatttt ctagtgtagg agatgacggc tggggaagct
    15721 tgaaagtaat tggtttctta gttgttggtg tgattttcat cgccttgttc tcatggcgtc
    15781 aattacattt ggaacatcca ttcttggaac ttcgtgtatt caaatcatct acattcacga
    15841 ttgcagctgt tttagctggt gtaacaaata tggcgatggt aggggcagaa atggtcgtac
    15901 cattgtatat tcaaaatatt cgtggagaat cagcattcca ttctggcttg atgttgttgc
    15961 cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg tgcgattttc gataaatacg
    16021 gcgcgaaacg tttagccatc acagggatgt tcattttgac agcggcaact gcaccatttg
    16081 ctttcttgac aaaaacgact ccagttttat atatcgtgat cctttatgcg atccgtatgt
    16141 ttgggatctc catggtcatg atgccggtaa cgacttctgg tatgaatgca ttgccaaata
    16201 acttgatcag tcatgggaca gcggtaaata atacattccg tcaaatcgca agctcgattg
    16261 gtacagctat tttgatcagt gtgttgacga atgtgacaaa agcaaatatc ccaagcaaat
    16321 ctgtattgga taatacgcca ttagcttata aagatcaggc aatcaatgcc acactgtcag
    16381 gttaccatgc cgcgtttatc gtagcgatca tctttgggat tcttggcttt attattgcct
    16441 ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg ggataatgca tgattattgg
    16501 agttttgatt gtcagcttgc ttgcttttgc tttaacgaac gtgttcgcta aaaaaacatg
    16561 gcaaaccttt ttatctttga ttttcggact aatcttcgtc gcttcactaa gtttgatcgt
    16621 cgctaatttg tccaatcact ttggaatgga aaaagtaacg gaaacaaaaa cagaaaaaat
    16681 tgtttccagt gctgattccc aaggagcaga tatgcttttg tataaagctt tagggaatgg
    16741 aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa gaaaaaccaa aagcaactgg
    16801 tacggataac gaaacaaata aagtagaaaa gacagacggc gatgctgaaa aagtgacgaa
    16861 aactacttat tgggaataca aaaacgacat gtataaattc tggttcaata ttgctgataa
    16921 caatcacgaa tatgacagcc gagtgaatac attcaaaatc ccagagacat gggtagaatt
    16981 aagtacggac caagcagcca agttagctga attagtgaag aaacagcaaa gtacgatgga
    17041 aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa gcaatgactg aaaatccaaa
    17101 aatgagcaaa gcagagcaag aacaacgtac gaaagcctta gctgctgaat tccaacaaca
    17161 agcattcgca aaattagtga aagaagcaaa aggcgaataa tattttgaac accatgtagg
    17221 aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca atcagataaa aatagcactc
    17281 catatttttc atctgattgg gcttagtgcc gtagatgcaa gcttttgaac accgtttaag
    17341 gaagaggttg tgagcctgcc gtttaactct gagtcacaag gagcaatttc aaaaaacagc
    17401 ttctcatgtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    17461 attcgaaaaa aggaactgcg acaagacttt tgtcacagtt ccttcttttt tttgctatgc
    17521 tatactagat gtaaaaaata ggagaaataa aatgagggaa atttattctt ttgaatcaaa
    17581 aatctatgct tccgaagtcg acaagggcgg ggcatatatt atttttccgt atgatataag
    17641 aaaagaattc ggtgctggaa gagttaaagt ccaagcaact tttgacaata tgccgtatga
    17701 aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt tcagtctgct acatccttgg
    17761 actacgaaaa gatattcgta aaaacatcgg aaaagaaatc ggtgatattg tagcagttac
    17821 agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt cacaacctct tgataaacgg
    17881 tgttcaaaag ccagtttctc ggtagtaagt caaatttatg caaaaatgtg gaaacccatg
    17941 tttacaaaat ttttcttact actcgaaacg aagcggcttt atcacaacct cttgataaac
    18001 ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg gcaaaatatg agaagctatt
    18061 tttccatttt tttctctcat actcaggtca taacgctgat ttcacaacct cttctttcat
    18121 aatgtttttt caccatcttt ataataaact ggttctttga tatggaaatc tattttttcc
    18181 aaccaatcta aaatcttgta acctaactct tctgacatcg gaaaagaggc gttgatttgt
    18241 gcattggaga tatggtcaac atggataccg gaagtgatcg tacaacttcc cggaacggca
    18301 gttttgatga tcacagctat tttttcggct aagacatcgt ctttatggaa ccgaccatcg
    18361 tggctaggga aacgtacagt ttgcatctct gtatcacgag aaaatgtagt gactgttccg
    18421 atatgtggtg tatctccgcc tgttaaagtg atcaataagt ctttaccgat tactttaaca
    18481 gcagcttgga tcgtgaaacc tagtttggtt acttcaacta tcgtttcttt ttccatatta
    18541 atcaccctgc caacgttttg aatgctcatt tacaattcca aattgatcca aaagtttcat
    18601 tgtatggtga ttaacaagat cttggatcgt ttgaggctga ttataaaaag caggaattgg
    18661 tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag ttttccaagt gaatggcatt
    18721 taatggcgtt tcacgaggaa caatgactaa ctttcgttgc tccttcattg ttacatcagc
    18781 agcacgtgca atcaggttgt cgcctaaacc aacagagata cctgcgactg ttttcatact
    18841 tgccggtacg atgatcattc catcggtcaa aaatgatccg ctggcgacag cagcacctaa
    18901 atctttgttt gaataatggt aatcaaacag gctttcgaat tcagttagtg ataagtcgga
    18961 ttccagcttt aagttctgtt ttgcccatgg actgatgatt ccgtgtgttt ctatcatcgg
    19021 atattctttt agtttcttca ctaggttgat ggcataaatg gtgcctgaag cgccacttac
    19081 accaatcacg atttttttct tccgttgtgc caattgttcc attttccgca ctccttttta
    19141 tgatgtcaaa acagttgcag atcattcatt atttcaaata tttttcccaa tcttctactt
    19201 cttggaattt tgcacgtaca aaatgttctt tcatatcgaa aggaactgta ccgtcaaaga
    19261 tagtttttgc actcatccca cggaaacgaa tagagccagg attatattct ggtaattctg
    19321 atgggtccaa tggatggttc ctcattcctg ggatcacgat gatatcttta tctccttgga
    19381 aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa aatatcaaca tcttcatcga
    19441 ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc aagaatagct gcttgacgct
    19501 gaataccttc atccgcttcg ttttctttac ggatttgcat gatggtcatc aatttaccac
    19561 cacctgcagg aggattgtag acatttacta cttttcctgg aatagcttta tcgaccaagg
    19621 caagaatact tgcttctgta ggaataccgg ccatagatac gtgttcttca ctgggaccaa
    19681 tcgttgtttg catgattgga ttgtcttttc ggtgagtaac tgctttgact ttcactactt
    19741 gtacagctgg attagcatcc ccagtatagc caggaaattc tggcattgct tttcctgagt
    19801 tcgtgttgat atcttcttgc attgtttcat ttggcatgat ttctgcttca atgatgaatt
    19861 cagcacgagc aatacccact tcatcgactg ctataccatc aacgatttga ataggttcgt
    19921 tacgcaaagc tcctgcacac catagttcat tgtagccaag tggagtggta ggtggttcga
    19981 aagtcgtgcc aatggcaatt gctggatcta aaccaatatt gatagtgata ggcattggtt
    20041 tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg gcgtccgcca ggcatgatgt
    20101 acataccgat cgtatcttta tcttctagaa ccatacgatg gatcgttaca tcgctcattg
    20161 ttttttctgg atctgatcct aagactacac ccattgtgat gtatggacca gcatcttcag
    20221 gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa gtctgcatcg gtagctagat
    20281 gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt cacaggtttg attggatttt
    20341 ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc atggtgcaaa atttcaccta
    20401 cacgtttacg actagccatt gttccgatgt tcacacgtgt gccggggaaa cctttgatat
    20461 tgttgaaagt catagctggt ccttcttgag tcggacgttt tactgtaccg ccagcaccga
    20521 tataacggta aacaccagat aattctgctt caggatcgat ttcaatatct gtttcgtgat
    20581 attgacctgg aagtgttttt agttcttcga tgacttttct taaatcatac ggttgatctg
    20641 tcatgagaga ttcctcctta gttgtcttta cctatctata gtaaaaggtg aaaaagagga
    20701 aaacaattca acttgttcgg taaagtattc tacaatggaa tggatgcgtt tttttgataa
    20761 tagaataaac tatgatatac ttatcactaa aagaaagcgc atacttagtg ttggaggaag
    20821 gaaagtgaaa aaaatgaagg agtcatttcc taaagcagta aaagtggaaa atatagcgaa
    20881 tattttgaaa gttacatttg aaaacggaga agtaaaatac gtgaaatctc attggacaga
    20941 agagataacc gatgccttgc aatttggaaa aaaaggaaga ggaaaaagaa aaaatcttct
    21001 cgcactttct cgtaatatgt ggattgggac tgaagtaacg attgaagcag atggaacagt
    21061 attcataaat ggaaaagaca ggtatacgcc agaagagttg tggtacaaag gaaagaaaag
    21121 tataccagaa ttataaaaaa taattaaaaa aaacagcgcg aaacaaggaa catattactt
    21181 cctcgtttct tgctgttttc tactatttca gcgctcttat gactcaaatc taaatgtggt
    21241 tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc ttttttcgct gccattcgtg
    21301 caggaagatg accgccaagc atcgtcaata tagtagagac gatcactaga ataatcgcat
    21361 ggacaggatt tagttgagca acatttttta gatccgtcat gttgtataag atgctattga
    21421 tagggaacgt agctagccat gcaataaaca cacctaatat cccagaagaa atacctaaaa
    21481 tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt acgtgcacct aaagctttta
    21541 gtacaccgat ttcttttgtc cgttcaatca cagatgtata agtaatgata ctgatcatga
    21601 tcatacttgt tactaaagag atccctgcaa aagcaataag gacataagtg atagcatcca
    21661 tcaatccgcc agtcagttca gtcatcgttc cagctaaatc agtataaatg attttatctt
    21721 catctgattt gcctttattg taatcatcta gataatccaa gatcttttct ttgtcttcaa
    21781 aattgttcgg ataaatcatg atacttgaag gcaagctgtc tccacctaaa taactaagca
    21841 atgtttgttt ggcattttcg tcaacttttt cagtagttaa gacatttaca ccactgtctc
    21901 tttgagcttt gacgatttct gattctttgt tttcgttgac gatttgagtc gttaattgat
    21961 cgctataagc gattcctgga gataagagat tcattgtaga agaagatttc acgcgtagga
    22021 tccccgagat cttcaattca tcagaagcat tctgatacat cgcatcataa tctgtattag
    22081 ggataaaatt cccagttgga agttttgtat agtaagtatt gttgtttact aatttgaatg
    22141 ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc tttcacatca aatccaagat
    22201 tcttcaatgc gttgatatta gtattattat tgccgtctac gatcagcacg acatctgttg
    22261 ctgaggctgg ataacttccg gcaagtaaag aataattgtc ttttaaaaag ttttctttgc
    22321 tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc tgtcattgct gacatcgtac
    22381 tagataaaga aagagattcc gtatcaggat tttgattcga gaagctgaca ggctgtactt
    22441 tgccatttac atcacgtaaa agattgattc ctgtagtacg ggtaaatccg atattattac
    22501 ttaagttagg atcgatgtct gttacgtaat cgacgtatgt ttgatcaatc ttattcgtgt
    22561 gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt tttgctgtct ggataatccg
    22621 ctttgcttgc acccaatccg gcatcgtcac gagtctggtt agtcgttacc ttagaaatag
    22681 taatcggaaa tttcgccatc gtttcagctt gcgtgttatc gatttgtttt tggaatccag
    22741 aagaaaggga cagaacgatc gcgattccga tgatcccaat actggaagca aaggaggtca
    22801 agaaagtacg tccttttttt gttcggatat tgttgaagga aagctttaac gctgtccaaa
    22861 aactcatctt tgttctgcgt aagttaaatt gatcatcttt tggtcgttcg atgtgaggat
    22921 gggaatctgt taagatctta ccatcagaaa attcaatgat ccgatctgcg tattgatggg
    22981 ccagttcagg attatgtgtg accatgatca ctaatttttc tttcgataac tcttggatca
    23041 attccatgat ctggatactt gtttctgtat ctaacgctcc ggttggttca tcgcacaaaa
    23101 ggatatctgg gtcattggct agtgctcgag cgatggccac tcgctgcatt tgtccaccag
    23161 ataactgatt tggcttttta tgcatatgat ctgtcaagcc gactcggtgc aatgcatctt
    23221 ctgcccgttt tcgtttttcg tctttagaaa caccacttaa tgtcatccca agttcaacgt
    23281 tttcaataat gcctagatga ccaatcaagt tgtaactttg aaaaatgaaa ccgatagagt
    23341 tgtttcggta ggcatcccag tcactgtcct taaagtcttt agtagacttt ccgttgatta
    23401 ccatatcacc ggaatcataa ttatctagtc caccaatcac attcagcata gtcgttttac
    23461 cagaaccact aggacctaaa atcgctacga attctttttg tctgaaagcg acagacacac
    23521 catccaatgc tttggtggtg gtttcgccga ctttataata tttttttata tcttttaatt
    23581 gaagcataat cacccttctc ccgtattgaa atattcggta tactaataag tatatcagaa
    23641 caatgatcgt agtatacgaa ataaatgtta actgaagatg aactacaaga aaaaatctag
    23701 aaatttttca aaaaacagag ggagatcaat gaatcgtatt ttagtagtag aagatgatcc
    23761 gcaattacag ttgctttacc gttctgtgtt ggaacgagcg ggcttttcag tcatcaccgc
    23821 attgaatgga gaggatgcgc tgaaaaaact agaagcgaca cgtatagaat taatcattac
    23881 agatatcatg atgccgaaaa tggatggcta tgacttgcta gactcattac gtagcaccag
    23941 atttgacaca cctgttctaa tgattacggc aaaggccgat tttgaggata agaaacgcgg
    24001 gttcaaatta ggagcagatg attatatgac aaaacccatc gatgtcaatg agatgatcct
    24061 acgtgtggaa gcattgcttc gacgttcaaa gatcaattac agcagtagct tgataatcgg
    24121 acaaacaaaa ttagagctag atgcttatac agtgttacaa gatcagcatt cgttgattct
    24181 gcctcaaaaa gaatttctgc ttctgtataa attactgtct tatcccaaca agatcttcac
    24241 tcgccaacag ttgatggatg atatttgggg attagatacg aatacagaag aacgaacgat
    24301 cgatgtacat atcaaacgtt taaggacccg ttttgaaaag acggaagatt ttcaaatcat
    24361 cacagtacgg ggactaggct ataaggcggt tatttaaaga tgaaaaaaaa gctattttca
    24421 caattatgga tctattttgc tagtattgtc ttcatttcga tcttagtgac actgctgtgt
    24481 tttctcggtt ttattttctt attgtctcga cattctatct cgcctgctga atcggctgga
    24541 aaaccaacgt tatacccgtt attcgtcttt gcggggttca gtatggtcgt gggtacagga
    24601 atatcgattt ttgttgggcg gcgtatcctg catccaatca gtgcgctagg gacaaatatg
    24661 agtctggtag caacgggaga tttttcgatt cgaatggatg aacagcagaa agtagcagaa
    24721 gtacagcaac tgtataaaga tttcaacgtt atggtccaag aactcaatag tatcgagaca
    24781 ctgaggaatg actttgtgtc cagtgtatcc catgaattca aaacaccact agcaacaatc
    24841 caaggatatg ttcaattgtt acaagcccca aatctctctg acgaagaacg ccaaatattt
    24901 ttatatcgga ttatcgaaag tatcacgcag ctttcacaat taacagagaa catcttaaaa
    24961 ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga aagagtatcg attagatgaa
    25021 caaatcagag aagtaatcgt ttttttgcag cctaaatggg agaaagaaca gctagaattg
    25081 gatatcgaac ttgctgcagt aaattacaca ggcaatgaag agtttttgta ccaagtgtgg
    25141 ctgaatatca tggataacgc cataaagtac aatcaaatca atggacagat tcatatcaaa
    25201 ctatttgaga cagccacaga gattggactg gaagtgaccg attcaggtgt aggaatgaat
    25261 gaagagacca gagatcgaat gtttgaaaaa ttttatcaag gagatacaag tcgtcaaatt
    25321 tctgggaatg ggctaggact ttcactggtt aaaaaaattc tagaactgca tgatggacgt
    25381 attgattata gcagtatcga aggagtagga acgacagcaa tgatacggtt aagtaaacaa
    25441 taggaaaaag aataaaaccc ctaaaaattc tattgaaggt ttttaggggt ttttagtcgt
    25501 ttttttgtga ttagggaaat gttcttacgg ttcttttggc ataaaaatcg ctgctttctt
    25561 attgaactac gagaaatgtt agaggtgtca ttgaaacaag gacatgatgc aagaaagaga
    25621 gcgaaagtca tgcgtcgctc tctttttttg ggtggataga tggacagaaa atgtagggct
    25681 tagttttctt cttgatttaa atactcccaa aatgtatctg aagccaaaga atgctcaccg
    25741 actttacgag taaatgcaag ataactctct gttgttggag aaatagggag aatcacagta
    25801 ttttctttta atagattctt gactgttttt tccataacaa tagcaatccc catgtcattt
    25861 gcaatcatat tgatgatcaa gtcgattctt gctcctttat aagagatttt tggttcaaat
    25921 ccagcttcgc gacacagatc tagtacaggt tgatacaagt tcgtagtggg gccaaggagc
    25981 aaaaagttct cctttttcag ttcgcttaga tccaatttct ttttactagc taaaggatga
    26041 tttttaggta gaactgctac aaaatcgtct gtttcagtag gcaaccactc aatcgaactt
    26101 ggtggggcct ggaaatttct agcaaagtgg atcgtgtcac tatgttcatt ggcgtcattg
    26161 taagttaatt cgacactttc cacttctttc aactggactg taaattcagg atggcgtttc
    26221 aaaaactcgg tgattttgga aaaaccttta taattgatca ttgtcggtat cgttaagata
    26281 tttaatgaaa gattgttgtc tttcgtatag gtttcaagca cttcttgcat agcatggtat
    26341 tgttctacga cattttttac atatgggagc aataagcttc cagcttcggt caattcgatt
    26401 tttctatggg cgcgttcgaa taatttgacg tctaattctg tttctaacga aagaatctgt
    26461 ttagaaatat ttccttgggt ggtgaattgt cgatcagcag ttttggtgaa attcattgtt
    26521 tcggctaaat caagaaaaac ttcgaattta tggatatcca tcatattcct cctacttatt
    26581 ccaataaaga atactttaac acaaaaaatg aattggtgta atcttctctg tctgttacaa
    26641 tctttcttgc agcgaaagca ataattacag aaatttttct gatttttttc gcttcgtaga
    26701 aaaagagagg agttttttga tgccaactgg agttttgatc aatgtaggtt ccgttttgct
    26761 tggcggacta attggcggat tagtaggaaa taaattaagt gaacatttta aagcacagct
    26821 gacgatggtt tttggtgtgt gttcgatggg gatgggaatc tattccattg cgccgatgaa
    26881 aaatatgccg gctgttattt ttgctttagt gataggaaca gcgattggtc tgattgttca
    26941 tttaggtaat gggataaata agggggctgc gctgatgcaa gtacctattt ctaaaatctt
    27001 ccctagtgaa aagcttggta tgacccacga tgaatttatc agcacattag tgacggtcat
    27061 tgttctattt tgtgcaagtg gtacggggat ctatggaagt ttagattctg ggatgacagg
    27121 agacagtacg attttgattt ctaaatctgt ccttgatttt ttcacagcag ctatttttgc
    27181 gtgtaattta gggtatgtcg tttctgtgat agcaattccc caattcatta ttttttatat
    27241 tttgttctta ttggcaaaat ttatttatcc attgactaca ccagacatga tcttagattt
    27301 taaagcttgc ggcggattct tgatggtcgc taccggtttt agaatgatca acgtgaaaat
    27361 gttcccagta gctgacatga tacctgcaat gatcgttatc atgccactga gctggatgtg
    27421 gacaaattgg attatgccac tgttataata aaaatcaaag agaagccatt ttttgctgac
    27481 taaaaaggat caaatctttt agtcagtttt tttgtatttg acaaactgat tttgaaacta
    27541 tattatatgt tgtataatat agattgaaat aaaatattac acttgagaat agaaggtgac
    27601 gtaatgcaca tccagattcc aactgttctt cttgatggta ctgtgctagc agcattaagc
    27661 aaggaagata tgtatgggta tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt
    27721 gaatctacac tatatccggt tttgagaaga ttgaaaaaag aaggatatct tgagacctat
    27781 gatgaacctt ttgaaggcag aaaccgtcga tattactcgt tgacaccaat gggaagtcgt
    27841 catttagaag aaattattga tgaatggatt gtttttcgag attctttaga ccggttattg
    27901 gaggagagca caaatgaatg aatatttgaa tgaagttgtg gatcatcttc aagaaattcc
    27961 tgaagacgat cgttttgatt tgatccagta ttatgaggaa tattttttag attctggaaa
    28021 aacattagat cagatcattg atgaatatgg tacaccaaag cagtttgcct taaaattaaa
    28081 aatcagttac ttttcagaag aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag
    28141 acaaattcgc ctgatttggt taatcattat cggtttattt gcttctcctg tcctcattcc
    28201 actagcactg gggtttatat tggttattgg gggactattg gttgctttac tagcgctcat
    28261 ttttagtatc tatgttttat gtatcagtat cttaggtgtt ggcctattca tgatcatctc
    28321 aggaatcagt gtgtttggac aatcgattct gagtggatta ttctttatcg gtgtcggtct
    28381 gttcgccaca ggaagcgcag tatttatttc gccactgttg ctgaaaggaa caaaagggct
    28441 tttttatgtc atgatgaact ttgttaaatg gatgggacga cgttttatta caaaaagaga
    28501 tctgcattca gcgggcgttt gaaggaggac acataaattg agattgaaac attttttgat
    28561 taccggtatt ggtctgatgc ttactggtgg cctcctagca ggtgcaactt atacattagg
    28621 cgctcaaaag agtctgactt gggataatgg tcctcggttg atcgatatgg ttactgagac
    28681 aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca gaaaatcaaa cagtttcggt
    28741 catacgcgga ttggattttg agataaaaac gacttatgat aagacagaaa agccagaaat
    28801 aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat
    28861 tagtttagaa gacacaagtg cacaaatcac tattgttgtc cctgcagatc atgaattatc
    28921 tgaattgaca atcgaaggga ataatagtgc aattgatatc aacgaagtat caatcaaaaa
    28981 agtgaatact aagacacatg atggctgggt atcgttcaat gaatttacag gaggaacaat
    29041 aactgcgaaa gatgaattag gctcacttcg attagatgag gccaatgtgg ataacttgat
    29101 cgtcacaggt gaaaacagca acttccatct agaagacagt ttgatcagca aagaaggaaa
    29161 agtcatccta aacggtggca atttatcttt gcatgagatg gaaaacagtg gctatactat
    29221 ccaaacgtct ggtgaatctt atattgaaaa agactaccag ccaataacaa atactttgtt
    29281 agaacaagga cagaaaaagc taaagatcac tgctaaagac acatcgatca atattacgac
    29341 ggatcaagag gacaatgaaa cagattgatc ctgtactcaa ctggatattt taagaaaaaa
    29401 cagcaaagat ccttccaatc tttgctgttt ttcatcgctt aacgcacata actatagtaa
    29461 aataagaaag aaaatatcta aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa
    29521 ggtcaaagag aaaagttaaa aacagaaatg gaaagattac atacttatat cactcaacta
    29581 agccaaacct tctatgatcc agacaaagaa aaagtgatgg ttaattaccc gaacaattca
    29641 gaaggtcgcc aattagagca agtgtaccat gaagttttca aacatttatt gacggtgaag
    29701 aaagagctgg attattattc tttaccgatt atcgatacag gaatattgaa gtatgatggg
    29761 aagaaggaac gatttatctt taaatctgtt cgggaagatt tgcctctttc agcagggatg
    29821 gatctagagg tcttggtaga agactatttt acagaagaaa agcactgggt tcgaacaaaa
    29881 ttggattatt taccgcaggc agccggaggg acacaggcaa ggggttggta catcactgaa
    29941 gacaaagagt tagaactaga aggtgtaatg gcgcgtattc gaaaaaaaat taaaaaagta
    30001 acattagata tttattgaag gataaaggag cgcaggaagt agcatgagag aaaaaagagc
    30061 attttatatc aatggttatg cagggattgt cggattggtc gtattagctt tagttggatt
    30121 gtttttcttc tatttaggta tgtggcaagc gaaagtttgg gcactttttt taagtatttt
    30181 tctttggttg atcacgttat tgttgttaag ttcggcaact gtagtcagtc ctaatcaagc
    30241 aaaagtaatt ttgttttttg gacaatattt aggtacgatt cgagaaaatg gctttttcct
    30301 tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa gttcggaatt tcaatagttc
    30361 tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa atctcagcag tagtagtctt
    30421 taaagtcgtt gatacagcaa aggcattatt cgatgtagcg tattatcaag attttgtaga
    30481 aatccaaagt gagacagcca ttcggcatat cgctagccaa tacccgtatg atacattcaa
    30541 tgagaatgat ttgacgcttc gaggaaacac gacggcagtg tctgacgagt tgcaaaaaga
    30601 gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc gaaacacgct tgaaccattt
    30661 agcctatgca acagaaatcg ctagtgccat gctccaacgt caacaagcaa aagccatttt
    30721 gtctgctcga caaaccattg ttgaaggagc tgtcacaatc actcaaatgg ccttagagca
    30781 aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg aaagtccaat tgatcaataa
    30841 tttacttgtc tcaatcatta cagacaaagg aacgcaacct gttatcaata caggcgacat
    30901 gaaagaccgt aagtgacata ctttttgtaa gataggaaga aggagtttag gacggattat
    30961 tgtcttgaac tcttttttta gttgttttaa agaaaatgac aaaattgaaa gaagaatgat
    31021 agttgattcg aaggcagaat tgttcgcttt tttctataat cagtatataa agtaagagag
    31081 aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt tttcatcgtt gtggtacttg
    31141 caggggtgaa aatcgcagat tacgtagtta tgggtggaga agaatactat gtacaaatta
    31201 cgacagatgg tacaaaaaca gtttcaaaat cagactctgg acaagaaaca gtcaactata
    31261 gatatatcct gcctggctat acgaaagagg gtaaggaaaa agaactggac ttcaagggac
    31321 aattgactcg accgcttcgt aaaggagcat atttaaaaat cacctggaac aaaaataaag
    31381 gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgtgcc gaaaacagca ctggaaaaac
    31441 taattaaagg gtagggaaag aaatgaacaa aatagtcgaa gtacagaatg taacaaaagt
    31501 atacggaaaa aacaatgaga aaaagacgca agcattaagc ggcatcagct tcgatgtaga
    31561 aaaaggcgaa tttattggga tcatgggcgc ttctggttct ggtaagtcga cacttttgaa
    31621 tattttatcc acattagata aaccaacaga tgggcatatc cgaatcaatc aaaaagatgt
    31681 gaccacatta aaaggaaatc aactagcaga ttttcgtgcg aatgagattg gctttatttt
    31741 ccaagatttc aatttattag aaagcttgac agcacaagaa aatatcgctg ttcctctttc
    31801 tttacaaggc gtcagaccaa aagaaatcaa gcaacgtgtc caaaaaattg cagaacgttt
    31861 atccatctct catattttgg aaagctatcc ttctgagatt tccggtggac aaaaacaacg
    31921 ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt ctattagggg atgagcctac
    31981 aggtgcgctg gattccaaaa gtgcccggga tcttttagat acgatggacg aattgaatac
    32041 aaaagatcat gtatcgattc ttcttgtgac gcatgatccg ttttctgcaa gttattgcca
    32101 acgtatttta ttcatcaagg atggcggtat ccatcaagaa gtgaaacgtg gagatcaaag
    32161 ccgtgattcc ttctatcgag aaatactatc tattcttgga aacttggaac agtaggggga
    32221 gattgactat gctttggaaa ttatcactta ctgggatcaa gagccgttta agagactact
    32281 tggtgttatt ctcaggatta gttatggcat ctgctatttt ttacatgttt gaatcaatgg
    32341 caagcaacga agcttttttg aaaagtaata cgatcgttgc ggctacagtt atcattttcc
    32401 gattcggttc tgttttacta gggatcatca cattcgttta tattttatat gcaaattcct
    32461 ttttgatgac gatgcggcaa aaagactatg caatgtttat gatgctggga gcaaaaggca
    32521 gaaaaatcgc acagatgatt ttttctgaaa cattcttagt agggatcgca gctactttag
    32581 tgggttcagc tgtcggtgta ggattgacaa gtatcgtcca ccgattatta gtggatcagt
    32641 tagatctcaa tatttcgcat tttacacctt ttagtattca cgggttgttg atcactgttt
    32701 tgttttttgc tgttttgttt ttattagctg ctgttgtcaa tgctttttcg attgtcaaaa
    32761 aaccgatttt agcattgatt cgtgcggatc agacaccaac aagaatcaag cagcataaat
    32821 tcttgttttt ccttgaagta gtactgggaa ttgcttttct aggctgcggt tactatatga
    32881 tgaaacacgt ggggacctat caagtctttg gtataggggt agccttagtt acgatcatat
    32941 taggaactta tttcattttc cattctgtga tcattttctt tttatcatta ctaaagaaat
    33001 cagaacagat ttcattgaaa aaattgaaca atttcacatt gtcacagtta aactttcgta
    33061 ttcgtgaata tacacaaatg ctctcaatgg tcgctatgct gtttgccttg gcactgggcg
    33121 ctttgacggt aggattaggt tttaaaaatg aaattgcaga cttgacagaa aacaattctg
    33181 gatatgatct agtgatgaat aatcctcggg cagaagatca gcaaaaaatc aaagaacttt
    33241 caccaacact ttctgcttct tatactcaaa aagaagatgc gggaacgatt tactataatg
    33301 caagtgaatt tgcggctcaa ccattgatca tgataaaaca tgaagatgga acaccgccga
    33361 agatcacgaa agaaaaagtt cctgctaaaa aaatgaatga actgactgta caagaggaac
    33421 ttcgaagcta tgaattgccg gaacagcaag aaaaagaaat caaggtagtc agtcaatcag
    33481 aatttgatca actgaacttg ccacaatcca ctttacagct agttcaggta aaagacttcc
    33541 aagcaaacct gaaaccaatc gaaacacttg ttgctcaaaa taaaatgaac aatccatctt
    33601 tgcaaaaagt agagtacagc aaccagaaat atgaaatgta tgaagtgtat aatggtctat
    33661 tctctggttt tgaatttatg ggattcttcc taggtttagc tttcttgacc atgcttgcta
    33721 gctgtttgat gttcaagatc ttgtcaggaa gcaagagtga cattgctcgt tatgaaatgc
    33781 ttgaaaagat aggaacacga cgcgggttgc ttaaacaatc gatccgtaga gaagtaggcg
    33841 tgttattctt agcaccagga atcttaggtg tagtccatgt cttattcgga ttacaaatgt
    33901 ttggtctatt catgcaaaat ccatacaaag atatttgggt tccgtttacg atcttcttcg
    33961 tattgtattt catctactat gtactgacga catggttata tactggaatc gtattgaaaa
    34021 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaagca aaaatagctc
    34081 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgtttg
    34141 ttcggaaaaa gaggttgtga caatattttt gtcacaacct ttttttcacg gtcttttatt
    34201 agaatatagt aaattttttc tcgttactca cggaataagc tgttaagaaa acggtttcca
    34261 tgtatactgg aatcagtgca aatgaaagaa aagagggtgc aaggaacatg gaaatcaatg
    34321 aacaaaattt tggcgaaaac tatcacttga ttacgatcac gaatcaaaat gggacatctt
    34381 tgtctgtcag tgatcttggg gcacgggttg ttcattttca aacaataatc gataatgaaa
    34441 aacgagagtt gatcttaggt tttgatgaag caaaagaata tctagaaaaa gacccttata
    34501 tcggtgcttc aatcggacgt atggctggac ggattacgaa tggcacattc aaattagatg
    34561 atgaaacgtt cgaagtaaaa acagacccgg acactggtca ttgtttacat ggtggtgcac
    34621 ctagctttga agccaagcag tggcattata cagtcatcaa tggtgaaaac gaagcatcgg
    34681 tcattttcta tcagactagc ccagatatgg gaaacggatt tcctggaact ttagatgtag
    34741 aagtacggta tacgctgacg aacgatgata catggcgagt gacggtccaa gggatcagtg
    34801 accgcaaaac agtattcaat cctacaaatc atgtctattt caatttaacc ggtgatccag
    34861 cacagtcaat cgacgaacat atactttggt tgaatagccg attatttgct acgctcaata
    34921 aagatacaac gcctacagga gaagtagttt cagtaagtgg cacagcattt gacttccaac
    34981 gtcctaaaaa attaggtgag gtctttgcca gtgattttcg ccaatcaatg atagtgggcg
    35041 gcatcgatca tccatttttg ttgaaaaaac aatctggttt actaaatgct gcggaattga
    35101 caagcccaga tgaaaaagta tcgatccaag taaaaacaga tgcttcagga atcgtgattt
    35161 ttacagcaaa ttttggagaa gatggaccgg aaatgcgcgg gaataaatta gtaaatcatg
    35221 gcggtatcac atttgagact caagaactgc caggagcgga gcgacttgaa agttttggag
    35281 atgtcactct ggaacctaac caagtacgcg agtttgtaac ggaatacaaa ataaatataa
    35341 agaaatagaa taaattaaaa agggagtgtg acagtgtcac atcccctttt taatttattc
    35401 tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa ggttagtaca
    35461 ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct tgccgtaaaa
    35521 ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt gttgacgcgt
    35581 tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca tttgattggc
    35641 acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt tgttgctgct
    35701 tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc gttcaatttt
    35761 tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc ttctttgtag
    35821 tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt cagtccattt
    35881 ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt acggaattgt
    35941 gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa ggtcacgctg
    36001 cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct tgataataag
    36061 ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc gtagattcct
    36121 tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg atcagttgtt
    36181 tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga gccgcctgtc
    36241 agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa atcaggcgtg
    36301 aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg gataaattgt
    36361 ccgttcccaa tatagatacc tacatggtga gtaacaccgg aagaaggtcc ccagaagacg
    36421 agatcgccag gttgggcctg acttactgga atctgcgtac ctgcatattg ctgttcgcct
    36481 gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt caaaccagaa
    36541 cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag atgcttgaat
    36601 gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac agcatcaatt
    36661 ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc gctttgattt
    36721 gctgtatttg gacgagatga agcattgttc gttccgccat tcgatgaacc attgtttgga
    36781 tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt tgtttgattc
    36841 gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga accattatta
    36901 ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt actagggact
    36961 tcttcactag gatcagttgg tttctctgtt gaagtatccg gtccagcttt aaattctgca
    37021 gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt tacttctttg
    37081 gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat agtgtaagtt
    37141 accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc caaaggttgc
    37201 tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa agaaggagca
    37261 attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc cggatcaatt
    37321 tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc ttctgtaaaa
    37381 gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt gactgtttga
    37441 tcagctattt gattatctac ggcaaatacg gtattggtcg acaaaacaga cggagcaatc
    37501 agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa ctagtccttt
    37561 tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg aacagataat
    37621 cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata aaacaatttg
    37681 taaataagca acgagcattc attcgttttt ctttgacgtt cattttattg catagaccac
    37741 ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa attataccaa
    37801 agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa aaagactgaa
    37861 atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa gacattaggt
    37921 cttcatcaaa cccgctaacg agagtagtga tcattgctac gactagacta acagccagtc
    37981 ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc agactaccaa
    38041 atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca gcagctgcac
    38101 caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa acagttggtt
    38161 cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa cgggtttttg
    38221 aacgtttact tcgcagaaat acacctaaag ctgcaccagc cattccaaaa ttggctgctc
    38281 ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc atgacagggt
    38341 ttacagccca ttggatacca aatattacca aaatattcca accaccagcg atgattgcgc
    38401 cactgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa ccttcgccaa
    38461 ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata actaatgaaa
    38521 gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg aatttttcaa
    38581 caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta tactgcatca
    38641 gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa agtaaagagg
    38701 tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca ttcactttaa
    38761 acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg gcattcgacg
    38821 cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc gtagtcgcta
    38881 aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc ggcgtaaaga
    38941 tttccgatat caaaccaaat agtttttcga tcggatttag cttttctttc ggagctgaat
    39001 gatcgatttg acgcgtcaat tccttgtaag caggagccaa tccctgatcc atgattattt
    39061 ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt tttaattttt
    39121 ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca catggaagga
    39181 cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc tgctcggaca
    39241 gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac tcactaatat
    39301 aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt tattcaactc
    39361 ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca ataatttgat
    39421 tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa tcgattgtac
    39481 ctcaagttct tctaaaacgt tttctaaaac agataaaaaa cttggaagac cgtttgagac
    39541 agccaaatct attttttcag taccaaaggg gacaggcatg cctgcatccc cgatacttaa
    39601 tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga ttacttttcc
    39661 ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt gttctaaagc
    39721 ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag aagaggctag
    39781 atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg tgctgcttaa
    39841 tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt taaaagcagg
    39901 gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta gtgtaatgat
    39961 tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac tgttatgatc
    40021 tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa tgatatctgt
    40081 cttttcaagt agttcagtag gaatcgtttc acgagctggt gcaggattca ggatcgttgt
    40141 ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc tactttcaaa
    40201 ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga cttcttgttc
    40261 atggaaagca ttatttgccc ctgcatggat caagatacta ttttctcctg ctgcgtctac
    40321 cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag atgtatcgat
    40381 gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc caacgcctcc
    40441 aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttggttgg cacctttacc
    40501 tccgccagcg tgaaaaatct catgcgcatg catcgtttcg cctggcttgg gcatgttggt
    40561 taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt tcattttaca
    40621 aacctccaac tatctcattt attttgatgg gaaaattatt ttttaaaacg ttttagtaaa
    40681 acgttttaat agttcgataa aaatatagca caccttgaaa gctatttcaa gggtgcagtt
    40741 gaaaatcttt tttctaatcg tacaggtaat cttttttctt cccattcttt ttctgggaat
    40801 tggatccgat ctaacaataa cttggcagat gtttgtccca attcgaagat tggttgtgcg
    40861 atcgttgtca gtttcggctt gatatactcg cacatatcta tattatcata gccaataatg
    40921 ctgtagtctt cagggatgct ttttccagct tcctctaatc cgcgatataa tccaaaagct
    40981 aattcatcat ttaaagcaaa cacaccagtt gcagcagaat caagaagttc tgctgtgatc
    41041 tggtagccac cttgttttga gaactgtgta ggtaagagga tcagttggtc gtgagggatt
    41101 tggtaaacat caagagcaga tttaaacccc tcaatccgag cgtggacatt ttccggtgga
    41161 ttttcaggat agacaagggc gatcgtttga tgtcctagac taagcaagtg catgcccgct
    41221 aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac taaatccttc agattttttc
    41281 tgatccaaaa caatgaaggg ccggccttgt tttttcaaat tttcgttgat tgcatctgta
    41341 gagacagcag aagtcgcgat aatgaatcca tccacacctc gacgtgtgag ctcggctaga
    41401 tattcgattt ctttttgatg atcagaatca gcattacata atatcgtgac gaaattctgc
    41461 ttatacaaga tatcctcaat tcctcgcatc agtgtactga agaatggatt cgtgatatca
    41521 ggaacgagta caccaattgt tttcgtttcc ttggtaatca tttgtctagc aaaatagtca
    41581 ggctgatagc ctaattcttc tttggcagct aaaactttct gaacagtttt cggactgaat
    41641 ttctccgtat ttccatttaa tatcaaggag acagttgtga ttgaaacgcc ggaaagttta
    41701 gcgacttctt tgattgtgat cttttttttc accatgttca tactcctatc taaatcctgt
    41761 ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat cacgtgtgcc agtacttatt
    41821 tgctttgttg gatagcttcg attatttgtt tcgttgagcg tactcgtgca gttttcggaa
    41881 atatcttttt tatgaacaat tgatgggatt ctttgtcacg atcactgcaa gcatcttcag
    41941 caagcacgat atggtacccg tgttgaaagg catctaatgc agtagcatat acaccgtttg
    42001 ttgtcgctac accgcttaaa atgatagttt cgattccacg ccggcgaagt tgaagatcaa
    42061 gagaagtacc aaaaaaagca cttggattgt attttttgat agtcaacata ttatctgtat
    42121 ctttcaaaga atccttcaaa agtaaatcag tgaattctgg aggtacagaa gcagtcattc
    42181 cacccatatc agtttgttga ctcaaataag ggaaagtgga gatgtctacg ttcactaaaa
    42241 ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa atcattgttt gtcagaatag
    42301 atgctgcagt gtgtggagag agtgtaccgt tatttacgat ctctttttgc atgtcgatcg
    42361 cgacaaaagc agtttttgat aaatcaaaaa tcatattttc ctttaaatca ttcatgatgt
    42421 tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt tatttgtcaa
    42481 ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat aaataagtgt
    42541 gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg aggaatcaga
    42601 gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga aacgaccatt
    42661 acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat tatacttggt
    42721 ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga tacacctgat
    42781 gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa acagcttact
    42841 gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt tcctaatcta
    42901 acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct tgatcctgta
    42961 gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc ccaattatca
    43021 ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc caaacttctt
    43081 ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt attgaaatta
    43141 ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca taattctgct
    43201 ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag gtctgttgaa
    43261 ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt tatgaaaaca
    43321 agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg attcattgca
    43381 atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac tgctggacct
    43441 gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga tttgcaaatc
    43501 gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat cccagatgca
    43561 aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga agtgattcaa
    43621 atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga tggaaaactc
    43681 ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg gtataaaatc
    43741 ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac catcacgggc
    43801 tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa cgtcggaaat
    43861 ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga tatttattct
    43921 gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa agcttacaaa
    43981 gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg acccgaagaa
    44041 cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca gcaaaaaata
    44101 gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc taaaaaacag
    44161 ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac aatcgctgtt
    44221 caaacattac ctcaacaaga agctcaactt gctgaacaag aaaggcaatt agttgagaaa
    44281 gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa aattgaaaat
    44341 ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt tcaagaattt
    44401 ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt ctttttcttt
    44461 attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa ccgcagggaa
    44521 atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa atatgcaatc
    44581 tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg aacgaatctt
    44641 ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag tgcgcttatt
    44701 ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc cgcatttggc
    44761 gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt gcttcaaccg
    44821 aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct gtggtcacgc
    44881 cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc aagaatgctt
    44941 atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt gggattgaaa
    45001 gattctctaa gttctgtttc agccaaacaa tttggaccga tcattgacta tcaagcaatc
    45061 gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat gctgcagaag
    45121 caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt gagaaaaaaa
    45181 ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga tttggatcgt
    45241 tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac aggaatcgct
    45301 attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat ttcgctttat
    45361 gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa ctatctaggt
    45421 agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc gatgatggat
    45481 aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat gctttccgag
    45541 aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca aatcgaaacg
    45601 caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt attatcagga
    45661 ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga gcgtgtacgc
    45721 gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat gtacattgtt
    45781 cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt aggatatata
    45841 ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc attagtgatc
    45901 acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt tattgtgatg
    45961 attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa atcgaatgaa
    46021 taaaaacaga ggttgtgaaa tcagcgtttt gagctaataa cgagggtcag ctgctaattg
    46081 aacaccatga atcagaggtt gtaaaaaagc cgtcctgcat cgagcaaaaa gaatactcaa
    46141 ataaaattag ctcctcatgt tttacatttg agaagactga ttgccgtaga taaaagcttt
    46201 tgaacaccat ttatagaaga aacaagaata ttttgctatt ttaaacaaga ggatagacaa
    46261 atacatgtct gatctcttgt tttttattgg gcaacagacc tcttagaatg tataaagata
    46321 aattttacaa acgctttctg ttcagttata ataataaatg ataacttgta tatataagct
    46381 gtggtaggta ataaatcagt tgaatggagg taaatatcca tgacatttaa agacaaagtc
    46441 atttaccaaa tctatcccaa atcttacaaa gacagtaatg gtgatggaat tggagattta
    46501 aaaggaatca aagaaaaact cccgtatctg aatgatctag gcatcgacat gatttggcta
    46561 aaccctattt atccaagccc tcaaaaagac aacggttatg atatctctga ttacacagcc
    46621 atcgatccta tctttggtac gatggaggaa ttcgaacagc taatagccga agcaaagaaa
    46681 taccggatag aaatcatgct cgatatggtg ctgaatcacg tttcgacaga acatgaatgg
    46741 ttcaaaaaag cactagcagg ggaccagcat tatcaggact tcttcatttt aagagaagag
    46801 ccaacggatt gggtatcaaa atttggtgga agtgcttggt cgccatttgg agatacgggg
    46861 aaatattatt tgcacttata tgatcggaca caagctgact tgaactggcg gaaccaagca
    46921 gtaagagagg aactattcaa agttgttcgt ttttggatgg aaaaaggggt aaaaggattc
    46981 cgtttcgatg ttatcaatgt tatcggtaaa gacgaacaat tgaaaaataa taaagaaaat
    47041 gatggcaaac ctgaatatac ggacaaacca atcacccata cgtatctaaa agaattgaac
    47101 caagcaacgt ttgggacgga ttccactatc atcacagtcg gtgaaatgag ctctacgacg
    47161 atcgaaaatt gtatcttgta tacagaacct agtagagaag aattatcaat ggtctttaat
    47221 ttccatcatt tgaaagtcga ttatgaaaat gggaataaat ggtctactcc tccatttgat
    47281 tttgcagaat taaagcgact atttcataca tggggagaaa atatgagtga gggtaacggc
    47341 tggaatgctc tattttttaa taatcatgat cagccacgtg cgctcaatcg atttgtagtt
    47401 atcgatcagt atcgaaaaca aggagcagag atgctagcaa ccatgatcca tttgaaccgt
    47461 gggacgccct atatttatat gggagaagaa atcggcatga tcgacccaga ctattcatcg
    47521 atttccgact atattgacgt agaaagtttg aatgcttata agttactcct tgaacaagga
    47581 ttcacagaaa aagaagcgtt tcgtcggatc aaagcaaaat ctcgcgataa ctctcgaaca
    47641 cccatgcaat ggtcagacac agaacaagca ggatttacta cagggaaacc ttggttaaaa
    47701 gttggaggca agctagaaga gattaatgta gaaaaagaac tatcttctga agattctatt
    47761 ttgtcctatt acaagaaatt gattcggctg agaaaaacat atccaatcgt tgcacaagga
    47821 gattatcatg cttatgaagc aaatcatccg gaagtttatg gctttcttcg ccaatttgaa
    47881 ggacagcagc tgcttgtatt aacaaatttc tatgataaag atacaaggat cgccatccca
    47941 gaaggattca cagaagccaa tattcttatc gataattatt ccgatactgt tcttcatcaa
    48001 agtctggagc taaaaccata ccaggcaatt gctttgctga aaaattaaaa caaaaacaca
    48061 aaacgtcgtt cctacgagct attctcgtaa gatcgacgtt ttgtgtttta tttaaaataa
    48121 tcttcttcag aaaatgaaga gttttcatac tctcctttat cgccataaaa tttattgtag
    48181 cgttgtttga atttacggaa aataaaagta acgagtaatt tgactaacgt atagatagga
    48241 ataccaaaaa taacgcccac aattcccatt agatcaccca tgacaagtaa aacgattaag
    48301 atcgtaatcg gatggatctg taaacgatca cccattactc gaggaatcac caagtcaccg
    48361 tggagaagtt gtacggcaaa ccagacaatg acaaatttga cagccataaa agtagagtct
    48421 tggaatgcaa tgatcaaacc tggaaagaag gcgataaacg gcccaatata tggaatgata
    48481 cataacaacc cagcagaaat agctaaaaca ctagcaaatt ctaatccgat cagtaagaaa
    48541 caaacccagt agactgctcc aaggataaat gaagcaatga tttgcccctt tagaaatgaa
    48601 cccaattgtt gattagcaat ctttgtcaaa tttttaaaat ctgtacgaaa tgctggtggg
    48661 acaatcgcta aaactgactg ataaaatttt tgaggatctc tcaaaaggaa aaaagcgaca
    48721 attggaccag taaataaagt aataaaaata gctgtaacag ttgaaaaaat gttgctgatc
    48781 cgttgtgcgc cactttccca ataatttcca ataaatccag ctatatcaga cgtcacgtcg
    48841 tcaatagaag caaaaaattg attaaatgaa tcggcaaaag gtgttttctc caaaaattgt
    48901 cggagactct cttgaaaatc ttgaaacaaa tctggcaaac tactgatcaa tgtctttgcc
    48961 tgttcttcaa gaaaaggaat aagccatatg cttccaagcc ccaataaaaa taaaataatc
    49021 acataaacga taatgactga caagttcctc gaaaggtgct tgctcaaacg tttgatgact
    49081 ggttccaaaa gataatacaa gatcagtgca aacaataatg ggggcaagat agtagcgata
    49141 atgattccga caggcttgaa tataaacgag atctgatgga aaaaataaat gatcagcgca
    49201 agtaagataa ctattcctaa aacatagtaa gaggtttttc ctcctaaaaa gcggatcaaa
    49261 cgattcgttg tatctgtttc ttttttcact ctttttcctc ctttatctat aacttcatta
    49321 tagacaaaag cgggtataaa aggaaaatga tagaggttga ttgcaaaaat atcgtcaatc
    49381 attcatataa actttctcat caattaaaat aagaaaacgc ttgacagttc ttttaaccat
    49441 atgataaatt aaatcctgtt gctaaaacgt aactattacg gtttgcgaaa agcgaatcgg
    49501 aacattcaaa tgaaagaaga tgagaagagg agtgtcataa atgaaacgat tgataggtat
    49561 attggccatg ctggttttag caggtatgtt aactgcttgc ggggcaagtg ggaaagccga
    49621 ggacagtaaa gaaaaattga gcgtgatgac gaccttttat cccatgtatg attttacaaa
    49681 agcaatcgtc ggggatgaag gagaagtaga gttattgatt ccagcaggga ctgattccca
    49741 cgattatgaa ccttctgcta aagacatggc aaagatccaa gatacagata tctttgttta
    49801 taatgatgaa aacatggaaa catgggtccc cgcaatccaa aaaacattac aagaaggcaa
    49861 tgtccatacg ataaaagcga cagaaggtat gcttttactt ccgggaagtg aagaaggaca
    49921 tgatcatgac catgaacacg gggaagaagg ccatacacat gagctagacc cacatgtatg
    49981 gctagctcca agtttagcaa tcaagcaggt agcaaacata cgtgatcagc tgattgaagc
    50041 ttacccagaa aaacaggaag tatggacaaa aaatgcagca gcctataccg agaaacttca
    50101 agcactccat cagctttatc aagaaacctt taagcaagca aaacaaagat cttttgtcac
    50161 gcaacatgct gcattcaact atctagcgtt agaatatgga ttgaatcaag tatcgattgc
    50221 tggtctttct tctagtgaag aaccgtctgc ggcgagaatt gctgaactga aacactttgt
    50281 taaagaacat ggaatcaact atatttattt tgaagaaaac gccaaagata gtattgccag
    50341 aacgttagca aacgaagcag gtgtatcttt agaagtattg aatcctttgg aaggattgac
    50401 gaatgaacaa atagaaaatg gagaaaacta tctttctatc atggaagcaa acctcgaagc
    50461 gttgaaaaaa acgacagaga cagaaaaccc gctagaagat actttgaccc ccaaaaaaga
    50521 aaagagcgtc tacaacggtt attttgaaga cgaggatgta caagatcgct ctctttcaga
    50581 ttgggcagga acttggcaaa cagtagatac gtatgtagaa gatgggactt ttgatcctgt
    50641 ttttgaatat aaagaaaaat tgaatcagga taagacagca caagaatata aagaatatta
    50701 tacgaagggc tatcaaacag atattgcttc aatcaagatc agtgagaaag agatggcatt
    50761 tacgtttaag gatggaacaa cgaaacgttc aaattatcgt tatgtaggga aagaaatctt
    50821 gacctacgag gcaggaaacc gtggcgtaag gtatctattc gaagcagaag ataaggaaag
    50881 tggcgctttt agatatattc aattcagtga tcacacgatt gcaccggctg actccgaaca
    50941 ttttcattta tatgcaggaa atgaaagtca agaagtattg ctgaaagaaa tggagaattg
    51001 gccaacattt tatccagata caatggatgg acgtgaaatt gcacaggaaa tgcttttgca
    51061 ctgataggca aaaaa
//
LOCUS       DAZKAS010000005        44999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000005 DAZKAS010000000
VERSION     DAZKAS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.005"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            336..1439
                     /locus_tag="ACG8H7_000211"
     CDS             336..1439
                     /locus_tag="ACG8H7_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFP8982352.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="ACG8H7_000212"
     CDS             1723..2403
                     /locus_tag="ACG8H7_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP8982353.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="ACG8H7_000213"
     CDS             2493..3527
                     /locus_tag="ACG8H7_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP8982354.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="ACG8H7_000214"
     CDS             3541..3876
                     /locus_tag="ACG8H7_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982355.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="ACG8H7_000215"
     CDS             3860..6340
                     /locus_tag="ACG8H7_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982356.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="ACG8H7_000216"
     CDS             6396..7955
                     /locus_tag="ACG8H7_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982357.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="ACG8H7_000217"
     CDS             7952..9316
                     /locus_tag="ACG8H7_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP8982358.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="ACG8H7_000218"
     CDS             9487..10557
                     /locus_tag="ACG8H7_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982359.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="ACG8H7_000219"
     CDS             10572..11240
                     /locus_tag="ACG8H7_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982360.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="ACG8H7_000220"
     CDS             11260..11838
                     /locus_tag="ACG8H7_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8982361.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="ACG8H7_000221"
     CDS             12241..13152
                     /locus_tag="ACG8H7_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP8982362.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /gene="rnhC"
                     /locus_tag="ACG8H7_000222"
     CDS             complement(13408..14325)
                     /gene="rnhC"
                     /locus_tag="ACG8H7_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFP8982363.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="ACG8H7_000223"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="ACG8H7_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP8982364.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15569
                     /locus_tag="ACG8H7_000224"
                     /pseudo
     CDS             15022..15569
                     /locus_tag="ACG8H7_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            15684..18044
                     /locus_tag="ACG8H7_000225"
     CDS             15684..18044
                     /locus_tag="ACG8H7_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFP8982365.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18131..18445
                     /gene="trxA"
                     /locus_tag="ACG8H7_000226"
     CDS             18131..18445
                     /gene="trxA"
                     /locus_tag="ACG8H7_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP8982366.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18627..20405
                     /gene="uvrC"
                     /locus_tag="ACG8H7_000227"
     CDS             18627..20405
                     /gene="uvrC"
                     /locus_tag="ACG8H7_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP8982367.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20550..21449
                     /locus_tag="ACG8H7_000228"
     CDS             20550..21449
                     /locus_tag="ACG8H7_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982368.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21442..22704
                     /locus_tag="ACG8H7_000229"
     CDS             21442..22704
                     /locus_tag="ACG8H7_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982369.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22867..24765
                     /locus_tag="ACG8H7_000230"
     CDS             22867..24765
                     /locus_tag="ACG8H7_000230"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFP8982370.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25064..25783)
                     /locus_tag="ACG8H7_000231"
     CDS             complement(25064..25783)
                     /locus_tag="ACG8H7_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFP8982371.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    25973..26020
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            26120..26452
                     /locus_tag="ACG8H7_000232"
     CDS             26120..26452
                     /locus_tag="ACG8H7_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982372.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26439..28442
                     /locus_tag="ACG8H7_000233"
     CDS             26439..28442
                     /locus_tag="ACG8H7_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFP8982373.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28461..28931
                     /locus_tag="ACG8H7_000234"
     CDS             28461..28931
                     /locus_tag="ACG8H7_000234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFP8982374.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28952..29536
                     /locus_tag="ACG8H7_000235"
     CDS             28952..29536
                     /locus_tag="ACG8H7_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFP8982375.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29551..30537
                     /locus_tag="ACG8H7_000236"
     CDS             29551..30537
                     /locus_tag="ACG8H7_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFP8982376.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30527..30838
                     /locus_tag="ACG8H7_000237"
     CDS             30527..30838
                     /locus_tag="ACG8H7_000237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFP8982377.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30860..32641
                     /locus_tag="ACG8H7_000238"
     CDS             30860..32641
                     /locus_tag="ACG8H7_000238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFP8982378.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32634..34010
                     /locus_tag="ACG8H7_000239"
     CDS             32634..34010
                     /locus_tag="ACG8H7_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFP8982379.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34017..34652
                     /locus_tag="ACG8H7_000240"
     CDS             34017..34652
                     /locus_tag="ACG8H7_000240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFP8982380.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34682..36037
                     /locus_tag="ACG8H7_000241"
     CDS             34682..36037
                     /locus_tag="ACG8H7_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP8982381.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36229..37623)
                     /locus_tag="ACG8H7_000242"
     CDS             complement(36229..37623)
                     /locus_tag="ACG8H7_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP8982382.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37715..38686
                     /locus_tag="ACG8H7_000243"
     CDS             37715..38686
                     /locus_tag="ACG8H7_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFP8982383.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38683..39465
                     /locus_tag="ACG8H7_000244"
     CDS             38683..39465
                     /locus_tag="ACG8H7_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFP8982384.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39634..40005)
                     /locus_tag="ACG8H7_000245"
     CDS             complement(39634..40005)
                     /locus_tag="ACG8H7_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8982385.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40139..40582
                     /locus_tag="ACG8H7_000246"
     CDS             40139..40582
                     /locus_tag="ACG8H7_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP8982386.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40625..41434)
                     /gene="yidA"
                     /locus_tag="ACG8H7_000247"
     CDS             complement(40625..41434)
                     /gene="yidA"
                     /locus_tag="ACG8H7_000247"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP8982387.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41453..42820)
                     /locus_tag="ACG8H7_000248"
     CDS             complement(41453..42820)
                     /locus_tag="ACG8H7_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP8982388.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42804..43634)
                     /locus_tag="ACG8H7_000249"
     CDS             complement(42804..43634)
                     /locus_tag="ACG8H7_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFP8982389.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43797..44234
                     /locus_tag="ACG8H7_000250"
     CDS             43797..44234
                     /locus_tag="ACG8H7_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFP8982390.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44310..44930
                     /gene="rpoE"
                     /locus_tag="ACG8H7_000251"
     CDS             44310..44930
                     /gene="rpoE"
                     /locus_tag="ACG8H7_000251"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFP8982391.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14743 a         7722 c         9570 g        12964 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagtgt ggtccaaaat caattcggcg
    15481 agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg
    15541 agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    15601 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    15661 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    15721 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    15781 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    15841 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    15901 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    15961 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    16021 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    16081 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    16141 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    16201 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    16261 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    16321 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    16381 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    16441 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    16501 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    16561 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    16621 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    16681 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    16741 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    16801 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    16861 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    16921 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    16981 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    17041 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    17101 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    17161 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    17221 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    17281 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    17341 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    17401 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    17461 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    17521 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    17581 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    17641 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    17701 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    17761 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    17821 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    17881 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    17941 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    18001 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    18061 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    18121 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    18181 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    18241 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    18301 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    18361 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    18421 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    18481 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    18541 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    18601 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    18661 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    18721 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    18781 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    18841 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    18901 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    18961 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    19021 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    19081 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    19141 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    19201 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    19261 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    19321 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    19381 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    19441 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    19501 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    19561 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    19621 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    19681 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    19741 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    19801 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    19861 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    19921 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    19981 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    20041 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    20101 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    20161 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    20221 gtaaaaacag ttttgcttcc cgattagatg aaattgaaag gttaggacca aaacgaaaaa
    20281 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    20341 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    20401 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    20461 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    20521 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    20581 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    20641 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    20701 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    20761 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    20821 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    20881 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    20941 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    21001 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    21061 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    21121 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    21181 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    21241 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    21301 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    21361 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    21421 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    21481 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    21541 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    21601 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    21661 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    21721 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    21781 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    21841 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    21901 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    21961 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    22021 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    22081 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    22141 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    22201 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    22261 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    22321 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    22381 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    22441 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    22501 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    22561 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    22621 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    22681 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    22741 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    22801 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    22861 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    22921 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    22981 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    23041 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    23101 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    23161 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    23221 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    23281 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    23341 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    23401 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    23461 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    23521 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    23581 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    23641 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    23701 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    23761 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    23821 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    23881 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    23941 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    24001 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    24061 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    24121 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    24181 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    24241 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    24301 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    24361 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    24421 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    24481 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    24541 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    24601 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    24661 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    24721 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    24781 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    24841 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    24901 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    24961 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    25021 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    25081 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    25141 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    25201 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    25261 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    25321 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    25381 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    25441 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    25501 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    25561 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    25621 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    25681 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    25741 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    25801 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    25861 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    25921 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    25981 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    26041 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    26101 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    26161 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    26221 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    26281 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    26341 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    26401 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    26461 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    26521 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    26581 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    26641 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    26701 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    26761 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    26821 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    26881 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    26941 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    27001 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    27061 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    27121 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    27181 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    27241 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    27301 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    27361 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    27421 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    27481 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    27541 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    27601 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    27661 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    27721 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    27781 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    27841 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    27901 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    27961 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    28021 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    28081 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    28141 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    28201 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    28261 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    28321 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    28381 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    28441 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    28501 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    28561 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    28621 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    28681 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    28741 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    28801 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    28861 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    28921 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    28981 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    29041 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    29101 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    29161 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    29221 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    29281 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    29341 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    29401 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    29461 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    29521 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    29581 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    29641 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    29701 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    29761 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    29821 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    29881 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    29941 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    30001 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    30061 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    30121 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    30181 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    30241 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    30301 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    30361 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    30421 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    30481 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    30541 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    30601 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    30661 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    30721 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    30781 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    30841 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    30901 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    30961 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    31021 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    31081 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    31141 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    31201 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    31261 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    31321 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    31381 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    31441 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    31501 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    31561 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    31621 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    31681 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    31741 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    31801 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    31861 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    31921 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    31981 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    32041 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    32101 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    32161 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    32221 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    32281 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    32341 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    32401 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    32461 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    32521 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    32581 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    32641 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    32701 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    32761 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    32821 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    32881 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    32941 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    33001 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    33061 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    33121 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    33181 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    33241 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    33301 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    33361 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    33421 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    33481 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    33541 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    33601 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    33661 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    33721 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    33781 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    33841 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    33901 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    33961 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    34021 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    34081 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    34141 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    34201 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    34261 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    34321 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    34381 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    34441 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    34501 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    34561 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    34621 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    34681 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    34741 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    34801 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    34861 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    34921 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    34981 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    35041 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    35101 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    35161 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    35221 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    35281 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    35341 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    35401 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    35461 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    35521 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    35581 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    35641 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    35701 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    35761 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    35821 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    35881 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    35941 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    36001 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    36061 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    36121 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    36181 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    36241 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    36301 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    36361 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    36421 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    36481 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    36541 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    36601 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    36661 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    36721 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    36781 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    36841 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    36901 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    36961 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    37021 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    37081 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    37141 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    37201 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    37261 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    37321 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    37381 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    37441 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    37501 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    37561 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    37621 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    37681 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    37741 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    37801 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg
    37861 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa
    37921 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    37981 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    38041 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    38101 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    38161 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    38221 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    38281 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    38341 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    38401 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    38461 ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    38521 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    38581 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    38641 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    38701 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    38761 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    38821 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    38881 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    38941 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    39001 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    39061 tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta
    39121 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    39181 aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt
    39241 gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca
    39301 cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat
    39361 caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata
    39421 ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa
    39481 ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac
    39541 gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt
    39601 ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata
    39661 accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc
    39721 agtaatctca agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc
    39781 tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt
    39841 caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc
    39901 tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga
    39961 tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct
    40021 tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca
    40081 tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat
    40141 ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc
    40201 atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt
    40261 ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga
    40321 gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga
    40381 gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc
    40441 tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag
    40501 ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg
    40561 gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta
    40621 tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag
    40681 tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac
    40741 cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt
    40801 gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat
    40861 ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt
    40921 gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga
    40981 cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta
    41041 aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt
    41101 catcgatcga atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat
    41161 cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg
    41221 ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt
    41281 cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag
    41341 cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac
    41401 catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata ttttaattta
    41461 tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata
    41521 aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat
    41581 ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca
    41641 aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat
    41701 aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc
    41761 cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa
    41821 aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag
    41881 gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga
    41941 ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt
    42001 caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa
    42061 ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc
    42121 taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat
    42181 aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga
    42241 aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt
    42301 ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac
    42361 cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta
    42421 cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat
    42481 gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc
    42541 ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg
    42601 aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga
    42661 aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca
    42721 aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt
    42781 tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta
    42841 ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt
    42901 agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca
    42961 aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc
    43021 attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat
    43081 tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac
    43141 atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca
    43201 tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc
    43261 ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg
    43321 gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct
    43381 gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt
    43441 gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg
    43501 cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat
    43561 atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga
    43621 atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac aatattatac
    43681 tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg
    43741 acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg
    43801 aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg
    43861 aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt
    43921 acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat
    43981 tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat
    44041 ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg
    44101 ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagga gggaaggttc
    44161 gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg
    44221 attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa
    44281 ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca
    44341 aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca
    44401 tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa
    44461 ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat
    44521 taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag
    44581 taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag
    44641 tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag
    44701 atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg
    44761 aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg
    44821 atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg
    44881 atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt
    44941 cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgat
//
LOCUS       DAZKAS010000006        44144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000006 DAZKAS010000000
VERSION     DAZKAS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.006"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..4466
                     /locus_tag="ACG8H7_000252"
     CDS             108..4466
                     /locus_tag="ACG8H7_000252"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFP8982392.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            4611..5084
                     /gene="rimP"
                     /locus_tag="ACG8H7_000253"
     CDS             4611..5084
                     /gene="rimP"
                     /locus_tag="ACG8H7_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP8982393.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            5107..6282
                     /gene="nusA"
                     /locus_tag="ACG8H7_000254"
     CDS             5107..6282
                     /gene="nusA"
                     /locus_tag="ACG8H7_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP8982394.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            6304..6597
                     /locus_tag="ACG8H7_000255"
     CDS             6304..6597
                     /locus_tag="ACG8H7_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFP8982395.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            6594..6905
                     /locus_tag="ACG8H7_000256"
     CDS             6594..6905
                     /locus_tag="ACG8H7_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFP8982396.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            6918..9224
                     /gene="infB"
                     /locus_tag="ACG8H7_000257"
     CDS             6918..9224
                     /gene="infB"
                     /locus_tag="ACG8H7_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP8982397.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            9251..9598
                     /gene="rbfA"
                     /locus_tag="ACG8H7_000258"
     CDS             9251..9598
                     /gene="rbfA"
                     /locus_tag="ACG8H7_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP8982398.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(9755..10211)
                     /locus_tag="ACG8H7_000259"
                     /pseudo
     CDS             complement(9755..10211)
                     /locus_tag="ACG8H7_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10376..12738
                     /locus_tag="ACG8H7_000260"
                     /pseudo
     CDS             10376..12738
                     /locus_tag="ACG8H7_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            13106..14029
                     /gene="truB"
                     /locus_tag="ACG8H7_000261"
     CDS             13106..14029
                     /gene="truB"
                     /locus_tag="ACG8H7_000261"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP8982399.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            14033..14974
                     /gene="ribF"
                     /locus_tag="ACG8H7_000262"
     CDS             14033..14974
                     /gene="ribF"
                     /locus_tag="ACG8H7_000262"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFP8982400.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            15326..16111
                     /locus_tag="ACG8H7_000263"
     CDS             15326..16111
                     /locus_tag="ACG8H7_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8982401.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            16124..16975
                     /locus_tag="ACG8H7_000264"
     CDS             16124..16975
                     /locus_tag="ACG8H7_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP8982402.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(17099..17551)
                     /locus_tag="ACG8H7_000265"
     CDS             complement(17099..17551)
                     /locus_tag="ACG8H7_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982403.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(18016..18384)
                     /locus_tag="ACG8H7_000266"
     CDS             complement(18016..18384)
                     /locus_tag="ACG8H7_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFP8982404.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            18534..19016
                     /locus_tag="ACG8H7_000267"
     CDS             18534..19016
                     /locus_tag="ACG8H7_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFP8982405.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            19053..20063
                     /locus_tag="ACG8H7_000268"
     CDS             19053..20063
                     /locus_tag="ACG8H7_000268"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFP8982406.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(20178..20891)
                     /locus_tag="ACG8H7_000269"
     CDS             complement(20178..20891)
                     /locus_tag="ACG8H7_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982407.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(21064..21671)
                     /locus_tag="ACG8H7_000270"
                     /pseudo
     CDS             complement(21064..21671)
                     /locus_tag="ACG8H7_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            22155..22382
                     /locus_tag="ACG8H7_000271"
     CDS             22155..22382
                     /locus_tag="ACG8H7_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP8982408.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22398..23564
                     /gene="hemW"
                     /locus_tag="ACG8H7_000272"
     CDS             22398..23564
                     /gene="hemW"
                     /locus_tag="ACG8H7_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP8982409.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            23798..24841
                     /gene="hrcA"
                     /locus_tag="ACG8H7_000273"
     CDS             23798..24841
                     /gene="hrcA"
                     /locus_tag="ACG8H7_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFP8982410.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            24935..25498
                     /gene="grpE"
                     /locus_tag="ACG8H7_000274"
     CDS             24935..25498
                     /gene="grpE"
                     /locus_tag="ACG8H7_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP8982411.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            25549..27378
                     /gene="dnaK"
                     /locus_tag="ACG8H7_000275"
     CDS             25549..27378
                     /gene="dnaK"
                     /locus_tag="ACG8H7_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP8982412.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            27529..28695
                     /gene="dnaJ"
                     /locus_tag="ACG8H7_000276"
     CDS             27529..28695
                     /gene="dnaJ"
                     /locus_tag="ACG8H7_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP8982413.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(28777..29406)
                     /locus_tag="ACG8H7_000277"
     CDS             complement(28777..29406)
                     /locus_tag="ACG8H7_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982414.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            29418..29591
                     /locus_tag="ACG8H7_000278"
     CDS             29418..29591
                     /locus_tag="ACG8H7_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982415.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(29636..30373)
                     /locus_tag="ACG8H7_000279"
     CDS             complement(29636..30373)
                     /locus_tag="ACG8H7_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP8982416.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(30375..31877)
                     /locus_tag="ACG8H7_000280"
     CDS             complement(30375..31877)
                     /locus_tag="ACG8H7_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFP8982417.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            32136..34130
                     /gene="uvrB"
                     /locus_tag="ACG8H7_000281"
     CDS             32136..34130
                     /gene="uvrB"
                     /locus_tag="ACG8H7_000281"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP8982418.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            34140..36956
                     /gene="uvrA"
                     /locus_tag="ACG8H7_000282"
     CDS             34140..36956
                     /gene="uvrA"
                     /locus_tag="ACG8H7_000282"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP8982419.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            37219..38484
                     /locus_tag="ACG8H7_000283"
     CDS             37219..38484
                     /locus_tag="ACG8H7_000283"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFP8982420.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            38488..39219
                     /locus_tag="ACG8H7_000284"
     CDS             38488..39219
                     /locus_tag="ACG8H7_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFP8982421.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            39354..40238
                     /gene="rapZ"
                     /locus_tag="ACG8H7_000285"
     CDS             39354..40238
                     /gene="rapZ"
                     /locus_tag="ACG8H7_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP8982422.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            40235..41233
                     /gene="yvcK"
                     /locus_tag="ACG8H7_000286"
     CDS             40235..41233
                     /gene="yvcK"
                     /locus_tag="ACG8H7_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP8982423.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            41267..42202
                     /gene="whiA"
                     /locus_tag="ACG8H7_000287"
     CDS             41267..42202
                     /gene="whiA"
                     /locus_tag="ACG8H7_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFP8982424.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            42745..43395
                     /locus_tag="ACG8H7_000288"
     CDS             42745..43395
                     /locus_tag="ACG8H7_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982425.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            43582..>44144
                     /locus_tag="ACG8H7_000289"
     CDS             43582..>44144
                     /locus_tag="ACG8H7_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFP8982426.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        14812 a         7594 c         9459 g        12279 t
ORIGIN      
        1 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
       61 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
      121 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
      181 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
      241 aatttacgct gcatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
      301 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
      361 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
      421 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
      481 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
      541 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
      601 tggatgagca gcaagcggaa cgagtattga aactttttga agaacgcaaa caagaacagg
      661 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
      721 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca catccagcta ggacgaaata
      781 ttccagccga tgaaccaacg acaccaatga tcagtatcgt tgaagaagaa cgaagagtca
      841 cacttgaggg gtacgtattc gacaaagaag tcagagaact acgttcaaaa cgcaagatct
      901 tgactttgaa aatcacagat tatacttcat catttatcgt caaaaaattc tcgaacggcg
      961 aaaaagatga acaagtattt gatgcgatca gtgtcggtag ctggttaaag gtacgaggaa
     1021 gtattcaaga agatacgttt gtccgtgatt tggtcatgaa tgcgcaagat atcatagaag
     1081 tcaaacatac acctcgcaaa gattatgctc ctgaagggga aaaacgagta gagcttcatg
     1141 ttcatagcaa tatgagtacg atggatgcaa caaacagcat ctctgatctt gtggcacaag
     1201 cagggaaatg gggacaccga gctatcgcca ttacagacca cggtggtgcg caagcctttc
     1261 cggaagcaca tagtgctgga aaaaaagcag gtgtgaaaat cttatacggc gtggaagcga
     1321 atgtcgtaga tgatggtgtc ccaattgctt ataatgatgc acatgaagca ttgagtgaag
     1381 ccacatatgt cgtatttgac gtggaaacga caggattgtc tgcagtatat gacacgatca
     1441 tcgagctagc cgcagtcaaa atgtataaag gaaatgtgat cgaaagcttt gatgaattta
     1501 tcgatccagg acatcctttg tcacgaacaa cggtagatct gactggtatc acggatggaa
     1561 tggttcgagg atcaaagtcg gaagaagaag tattgcggat gttccttgaa ttttcaaaag
     1621 atacaatttt ggtagcccac aacgcggctt ttgatatggg gttcttgaat actagctacg
     1681 caaggtatgg tattcctgaa gcggccaatc cagtcattga tacgttggaa ttagctagat
     1741 atctgtatcc tcaattcaaa cgtttcggat taggtgtgtt atctaaaaaa tttggtgtca
     1801 gcttggaaca gcatcaccga gcaatctatg atgctgaagc aactggtcac ttagcatgga
     1861 tctttgtgaa agaggcaatg gacaatcata acatgcttta tcatgatcag ctaaatgagc
     1921 atatcggaga aggagattct tacaaaaggg ctcgaccttt ccatgtaacg attttagcaa
     1981 aaaatcaggc aggactgaaa gatttgttca aactgatttc aatgtcgaat gtcgaatact
     2041 tcgaacgcgt cccacgaata ccacgctccc aattgaaaaa gatgcgtgaa aatctattga
     2101 tcggctcagc ttgtgataag ggagaaatat tcgaagccat gatgcaaaaa ggggtagaag
     2161 aggcaagaaa ccgagcaaaa ttctacgatt atattgaagt tatgccaaaa gcggtctatg
     2221 cgcctttgat cgagcaagaa ttggtaaaaa atgaacatga tttggaagaa attatccaaa
     2281 atttggtaga aatcggaaaa agtttggata aaatcgttgt tgcaacaggt aatgtacatt
     2341 atctgaacga agaagatgcc atttaccgaa aaattttgat caattctatg ggcggagcga
     2401 atccgttgaa tcgtcatagt ttgccggatg tccatttccg aacaaccgat gagatgctaa
     2461 ctgcatttca ctttttaggg gaagaaacag cgaaagagat cgtagtagaa aatacgaata
     2521 aaatcgcaga tatctgcgaa gaagtgattc cagtcaaaga tgaactttat acaccgaaga
     2581 ttccaggttc agaagatgaa atatcagaat tgagttacac aaaagcaaaa caaatgtacg
     2641 gagatccgtt acctgagatc attcaaaaac gattaaaaaa agagttgaat tctattaatg
     2701 gtaatggctt ctcggttatt tatctgatcg cccaaaaact agtgcataaa agtaatgaag
     2761 atggctatct agtaggttca cgtggctctg ttggctctag ctttgttgcg acgatgacag
     2821 gaatcactga agttaatccg ttagcccctc attattattg tccagagtgc caatattccg
     2881 agttttttga agatggtacg tatggttctg gatttgacat gccagaaaaa caatgtccaa
     2941 aatgtggtgc acgcttaaac aaagacggtc atgatattcc atttgagaca tttttaggat
     3001 tccatggaga caaagtgccc gatattgatt tgaacttctc aggtgattat caagcagaag
     3061 cccataacta tacgaaagta ttgtttggcg aagactatgt ttaccgtgcc ggaacgattg
     3121 gtacagtcgc cgacaaaaca gcatatggct atgtaaaagg atacgaaagg gacaataatc
     3181 tgcagtttcg aagtgcagaa gtcgacagat tagcaaaagg cgctactgga gtcaaacgga
     3241 caaccggcca gcatccaggg gggatcatcg ttattcccga ttatatggat gtctatgatt
     3301 ttacgccgat ccaatatcca gcagacgatc aaaattcgga atggaaaacg actcattttg
     3361 attttcactc gattcacgat aatgtattga aattagatat tctagggcat gatgacccaa
     3421 ctgttattcg gatgcttcaa gatttatcag ggatcgatcc acaaacgatc cctacggatg
     3481 acccggaagt gatgcggata tttgccggtc ctgaagtact tggagtaagt caggaacaaa
     3541 tttattccaa aacagggaca ttaggtattc cagaattcgg cactcgtttt gttcgaggga
     3601 tgctagaaga aacgcatcct acaacttttg cagaactact gcaaatttcc ggactatctc
     3661 atggtacaga cgtatggtta ggaaatgcgg aagaattgat tcgacgagga gatgcgacac
     3721 tggcggaagt aatcggctgt cgtgatgata ttatggttta tttgatccat gctggattag
     3781 atagcgggat ggcatttaaa atcatggaaa ctgttcgtaa aggacaatgg aacaagatcc
     3841 ctgatgaatt gcgagaaacc tatctatcgg cgatgaaaga aaataatgtg cccgactggt
     3901 atatcgattc ttgttcaaaa atcaaatata tgtttccgaa agcccatgct gcagcttacg
     3961 tattgatggc tcttcgtgtc gcttatttca aggtttattt ccctattttg tattactgtg
     4021 catacttttc tgtacgtgca gacgactttg atctagtctc catgtgcaaa ggaaaagatg
     4081 ctgtgaaaca agcaatgaaa gaaatcacgg acaaaggatt agatgcttcc gtaaaagaaa
     4141 agaatcaact gacagtgctg gagttagcca atgaaatgct ggaacgagga ttcaaatttg
     4201 gaatgattga tttgtataaa tcggatgccg ttaactttgt tattgaaggt gatacgttga
     4261 tcgcaccatt tagagctgtc ccaagtttgg gaaccaacgt ggcaaaacaa atcgttgaag
     4321 caagaaaaga cggtccattt ttatccaaag aagatcttgc cacccgcggt aaagtttcaa
     4381 aaacattgat cgaatatatg aacgacaatg gcgtattgaa agatctacca gatgaaaatc
     4441 aattatcgtt attcgatatg ctataagcat ctgttttcct gtctgcttgt aattttaagc
     4501 agagtatggt atagttctta taggtaatga tactaactgt gagtgagcgg atttttcgct
     4561 cactcttttt aatggaatta cgcacttaat tttttaagga ggcgaaactt ttgagtagcg
     4621 tcgttgaaac agtcacagaa atggtgacac cgattttaga tgaacaaaat tttgaacttg
     4681 tcgaagtcga atttgtaaaa gaaggaaaaa gctggttctt acgagtattt attgacaaag
     4741 aaggcggtat cgatatcgaa gaatgcgcct ttgtcagtga gaaactgagt gaaaaacttg
     4801 atgcagcaga tccagatccg attcctcaag cttatttcct ggaggtatcc tccccgggag
     4861 cagagcggcc attgaaaaaa gaaagcgact atgaaaaggc acttggtgat tatgtccatg
     4921 tctccttata ccaaccagta gatggcgaaa aacaatatga aggatttctt caatcatttg
     4981 atgctgaaca gcttacattg aaaatacgca taaaaacacg agaaaaagaa ttgacttttg
     5041 accgaaaaaa tattgcaaaa gctcgcttag ctatccaatt ttaatcacgg agaggaatca
     5101 gaaaaaatga gtaaagaaat gttaaatgcg ttagacgctt tagaagctga aaaagggatc
     5161 tcaaaagaaa tcgtgatcga tgctttagaa gcagcattgg tttctgcata caaacgtcac
     5221 tatggccaag cacaaaacgt cgaagtggaa tttgatcaga aaaaaggaaa gatccatgtc
     5281 tatgcggtaa aagaagtaac ggaagaagtg atggattcac agttggaagt ctcattgaaa
     5341 gatgcacttt taatcaatcc agcatatgag atcggtgata cgatccgttt tgaagtaaca
     5401 ccaaaagatt ttgggcggat cgctgcgcaa acggctaaac aagttatttt acagcgtgta
     5461 cgtgaagcag aaagaacgat tatttataac gaattcagtg cttatgaaaa agacatcatg
     5521 caaggaatcg tcgaacgcca ggataaacgt tatatttatg tcaatcttgg taaaattgag
     5581 gcagttcttt ctaaacaaga tcaaatgcca aatgaatttt atcaaccaca tgatcgaatc
     5641 aaagtttatg tttcccgagt agaaaatacg tctaaaggtc cgcaagtatt tgttagccgc
     5701 agccatccag acctattgcg ccgcttattt gagcaagaag taccagaagt ctatgatgga
     5761 atcgttgaaa ttgtcagcat cgcccgtgaa gctggcgatc gttcaaaagt ggcagttcgc
     5821 tcaaatgatc caaatatcga tgcagtcggt acatgtgtcg ggccaaaggg acaacgagtt
     5881 caagcaatcg tcaatgaatt aaaaggcgaa aacatggata tcgtcgaatg gaatgaagat
     5941 ccagctgtat tcattgcaaa tgcattgaat cctgcacaag tcatggatgt gatcttcgat
     6001 gagacaaatc caaaggcctg cacagttgtc gttccagact accaattatc gctagcaatc
     6061 ggtaaacgcg gacaaaacgc acgtttagct gctaaattga ctaaccataa aatcgatatc
     6121 aaatcagaat ctgacatgac ggaattttat gaaaatcaag aacaacaaaa agaaacagaa
     6181 gaactgcatg atgaagcaat cgttcagtca gatttaacag acgatgaata tgaaacaatt
     6241 gcattcaaca atgaaacggt cgaagaaaaa ccagaagctt aactttagta aggaggcaaa
     6301 gagatgaaac aaaggaaaat tcctttacgc aaatccgtgg tttcaggtga aatgaaacct
     6361 aaaaaagaga tggtccgaat cacccgttca aaagaagggg aagtatcgat cgatccaact
     6421 ggaaaaatgc ctggacgagg agcgtatgtc tcacttgagc cggaagaagt acaacaagct
     6481 tgggataaaa aaattctcga tcgagtactc gaaacaacat taacagatga gttttatcaa
     6541 gaacttcttg actatgtcac tcaccaaaaa gcacgtaaag agttatttgg aaaatgaata
     6601 cagttaatcg gcaaaaagcc atgaatctca tcggtctagc gatgcgcgcg ggaaaactaa
     6661 tcacgggtga agaactgacc attgctgaaa tccgcaaaca aaaagcaaaa ttggtgtttg
     6721 tggcaaccga tgcaagcgag aatacaagaa aaaagataaa agataagagt tcatattatg
     6781 aagttccttg ctttgagcta ttttctgaag cagaaatcac gcagatgatc ggaaaacctc
     6841 gcaaggtatt cggaataatg gatgccggat ttgcgaagaa gaccaaggag ctaattgaag
     6901 gttaggaagg tgattgcatg ggcaacaaaa gaatctatga attagcaaaa gaactcaaaa
     6961 aatctagtaa agacgtcgtg gaaaaagcac aacaacttgg catcgatgtg aaaaaccata
     7021 tgggtgcgat tactagcaat gatgagaaga aattgcaaca agcgttcaaa aacccaagca
     7081 cgaaacaaca agctgcacaa caagcaagca gaaaaccagc caatcaacag ccgaacgatc
     7141 aacgtaaaaa agaaacaacg aacaatcaac aaaaaaataa aaataaccgc agttatcaag
     7201 atcgcggtca agggagcggt caagtgaatc aaggaaaaaa accaacaaca aaccaaaata
     7261 atacgggaaa taccagcagc caaaatcgtc aaggaacgag ccaaggcggc aatcaaaacc
     7321 gtactaacaa taacaacaaa ccaaacaata acaatcgcgg caaatttaat aataataacc
     7381 gtaatcgtta taataaaaaa ggtaaaaaag gcaaacagca acaatcaact aaaccagctg
     7441 taccaccacg caaattccgt gaattgccag atgtattaga gtatactgaa ggtatgaacg
     7501 tagcagatat tgcgaaaaaa attcatcgtg agccagcaga aatcattaaa aaattattta
     7561 tgcttggtgt catggtcaat caaaaccaag cactggataa agatacgatt gaactgttag
     7621 cgacggacta tggcatggaa ccacaagaaa aagtccaagt tgacattgct gatatcgata
     7681 aattcttcga ggcagaagaa gtcgatccag ataaattagt ctcacgtccg ccagtcgtaa
     7741 cgattatggg acacgtagac catgggaaaa caactttatt ggatacatta cgccattcac
     7801 gtgtaacaag cggcgaagcc ggcggaatca cacagcatat cggtgcctac cagatcgata
     7861 ttgatggtaa accaatcact ttcttggata caccaggaca tgcggccttt acaagtatgc
     7921 gtgcacgtgg agcaagtatc acagatatca cgatcttagt tgttgctgca gatgatggcg
     7981 tcatgccaca aacagttgaa gcaatcaacc atgccaaagc tgcaggcgtt ccaatcatcg
     8041 tcgctgtaaa taaaattgat aaaccaggtg caaaccctca acatgttatg caagaattaa
     8101 gtgagtatga gttaattcct gaagcatggg gtggagaaac gatctttgtt gagatctcag
     8161 cgaaattcgg ccaaaacatc gaagaattgc tggaaatgat cctgttagtc gcagaagtag
     8221 aagatttaaa agcagatcca acacaacgcg caatcggaac agtgatcgaa gcacgtcttg
     8281 ataaaggtaa aggtcctgtg tcaaccttac ttgtccaaga aggaacactt catgttggtg
     8341 acccaatcgt tgtcggtaat acttacggac gtgtccgtgt aatgacaaac gatcttggcc
     8401 gtcgcgataa agaagcagga cctgcaacgc cagtagaaat cactggcttg aatgatgtac
     8461 ctcaagcagg tgaccgtttc gtagtcttcg aagacgaaaa gacagcacgt gcagcgggtg
     8521 aagaacgcgc gaaacgtgca ttattagaaa accgtgctgc cacaagccgt gtgactttgg
     8581 ataacttatt tgaaagcctg aaagaaggcg aattgaaaga agttaacgta atcatcaaag
     8641 cagatgtaca aggttcagca gaagcactgg ctgcatcatt gaagaagatt gatgtggaag
     8701 gtgtccgtgt aaaaatcgtc cattcagctg tcggagcaat caatgaaagt gatgtaacct
     8761 tagcagcagc cagcaacgct attatcatcg gtttcaacgt tcgtcctaca ccgcaggcaa
     8821 aagtgcaagc tgatactgaa gaagtcgata ttcgtcttca ccgtatcatc tataaagcca
     8881 tcgaagaaat cgaaactgcg atgaaaggga tgctagaccc tgaatttgaa gaaaaaatca
     8941 ccggtcaaat gatcgttcgt gaaaccttca aagtttctaa agttggtacg attgcaggtg
     9001 cctatgtaac agaaggatat attcgtagag atagcggtgt ccgtgtaatc cgtgacggaa
     9061 tcgtaatcta tgaaggccaa cttgcaagtc tgaaacgatt caaagatgac gtgaaagaag
     9121 tcaaaatggg ttatgaatgt ggtgcaatga ttgaaaaatt caatgacatc aaagtagatg
     9181 acgtaattga aggatttatc atggaagaag tcaaaaatga ctaaaaactg ataaaaggag
     9241 gccaatcatt atggctaact accgtgaccg ccgagtcggt caagaaatct taaaagaagt
     9301 caatgatatt ttgcgcaaaa aggtccgtga ccctcgtgtg gagaacgtaa cgatcacaga
     9361 tgtgaaagtt acgggggact tacagcaagc aacgatctat tacagcattt tatctgactt
     9421 agcatcagat aagaaaacag cacaagatgg attgaataaa gcatcaggat tgattcgtcg
     9481 agagttaggg cacaatctaa gtatttataa gacacctgaa ttgttctttg aacttgatga
     9541 atcagttgtc tatggcaacc atatcgatga attgcttcgc gacttgaaca aagactaatc
     9601 aaaaactccc gcaaatcctg tgaaatcagg ggttgcggga tttttttgtt ttcattaaga
     9661 cgtattacct aagagttcca tgactttagt cttgcggcgt gtcagctaac caaaaaagag
     9721 aaaaagaaaa gtaaaaaatt cgactttctt ttttctagta gtatataaat ttttgcatat
     9781 agataaagat caagataccg ataacggaag ctagattgta atctatttta gtgacagtca
     9841 ggtatagcca agtaactaga agatcagtgg aacctgcacc ataaaagttt gagtttgtcg
     9901 gtccgttgac agttaaaaga aaaatcacgt tgaagttatt gatatttccg attacgccgc
     9961 ttcgatttgg tcacgatcca aattttggat aatccctgta gaaattagca tagtagctgg
    10021 gataccaatt cacatattga caaagataac agagactttt acataaaacg gatcaatcag
    10081 gaaaggaatt gctcgatcca gccccagttt tccagcaatg tgttaactgg cccagcgtca
    10141 ttcagcaaat tatgcatgat gaggagcgaa atgaactgcg gaacagccat tgtgataacg
    10201 acaagttttt tggatcaatg taaaaaacgt cagcgaaagg aaaaatttcg tggtttttta
    10261 tacctgttca tttggctaga tataaaatcc aaacaaatga acagaagtgc ttattaggtt
    10321 tcaattggga gtatctgcgc tagaataaga gtaataatga aaaggagtgt tatgtatgga
    10381 ttattctagt aaagaatact ttgataaaat gacggcttgg tggcgtgctg ctaattatct
    10441 ttctgttggg caactttatc ttaaagataa ccccttactg agacgtacac tgaaacccga
    10501 agatgtcaaa aaacatccaa tcggtcattg gggaacgatt cctggccaaa attttattta
    10561 tgtccatttg aaccgagtca tcaataaata tgatttgaac atgttttata ttgaagggcc
    10621 aggacacggc ggacaagtca tggtatcgaa tgcttattta gatggaagtt acacagagat
    10681 ctatccagaa gtaacggaag atgagacagg aatgcaaaaa ttattcaaac gattttcatt
    10741 tcctggaggc attgcttctc atgcagcacc agaaacacca ggttctatcc atgaaggcgg
    10801 agagcttgga tactctcttt cacatgcagt cggagctgta ttagacaatc ctgaagtaat
    10861 ctctgctgtc gtgattggtg atggtgaagc agaaaccggt cctttagctg gaagctggtt
    10921 ctcaaatgtc tttatcaatc cagtgataga tggagctgtt ttaccgatcc ttcatttaaa
    10981 tggtgcaaaa atagccaacc caacgatctt ggcacgtaaa tcagatggag aattagcaaa
    11041 ttactttaac ggacttggct gggaaccttt ctttattgaa ggaaatgatt cagaaaaatt
    11101 aaatcctgtt atggctgaaa aaatggatca agcaatcgag aaaattaaat ccatccaaaa
    11161 agaagctcgc ctgaagacag caacagatgt cgtgatgcca aaatggccag tattgatcgt
    11221 acgcacacca aaaggatgga ccggtccaaa agaatgggac ggggaaccaa ttgaaggaac
    11281 cttccgagca caccaagtac cgattcctgt agatcaagaa catatggatc atgcagatgc
    11341 tttactccgt tggctgaaat cctatgaacc agaaaaatta tttgatgcac aaggacgtat
    11401 tttagaagaa atcagagaaa tcgcgcctac tggtgaccaa agaatggcaa aaaatcctat
    11461 caccaatggt ggaatcgatc caagaccgct tattatgcca gattggaaaa aatatacttt
    11521 gcaatttgaa aaaccaggct ccgttaaagc agaagatatg accgaacttg gtaaattcgt
    11581 tcgcgagatc attgagaaaa atccagaaaa tttccgtata tttggaccag atgaaacaaa
    11641 atcaaaccga ttgaatcaag ttttcaaaac gacaaatcgc caatggatgg aaaaaatcga
    11701 accagaaaat gatgaatggc tttcgccatc tggtcgcgtc atcgacagtc aattatcgga
    11761 acaccaagat gaagggttct tagaagggta cgttctgact ggacggcatg gttttttgca
    11821 agttacgaat cattcttgcg agtggttgac tctatgttga ctcagcactt caaatggatg
    11881 cggaaatcac atgacctttc ttggcgaaat gattatcctt cgctaaatct cattgcaagc
    11941 tcaacagtct tccagcaaga tcacaatgga tatagccacc aagatccagg aatattgaca
    12001 catttggcag aaaagaaggc ggagttcatc cgtgaatatc tgcctgcgga tgcaaataca
    12061 ttgctcgcag tcatggataa agcattccgc tcgtcagaaa aaatcaattt gatcatctct
    12121 tccaaacatc cgcgtgctca attttactca gcagaagaag cagccgtctt agtgaatgaa
    12181 ggcttgaaaa tcattgattg ggcttcgaca gcaaaagagg aggaacctga attggtcatt
    12241 gctgctgcag gaacggaatc taacttagaa gcattagcag ctgtaacttt gctgctggaa
    12301 gaattcccaa aactaaaaat tcggttcatc aatgtcgtag atttattgaa attacgtcat
    12361 ccaagtcaag atccgcgtgg gttgagcgat gaagaatttg atcaatattt tacaaaagac
    12421 aaaccgatct tatttgcttt ccacggttat gaaacattgg tccgcacgat tttctttgat
    12481 cgtcataacc atcatttaat gattcatggc tacaaagaaa atggagatat cacgacacca
    12541 tttgacatgc gtgtagtaaa tgaacttgat cgttatcatt tggcaaaaga cgcagcttta
    12601 aaaatcaaag gaagtcaagc agaagatttt gcgaaaaaaa tggatcagaa attacaagaa
    12661 catcaaaact atattcgtga aaatggtatt gatctgccag aagtattgga ttggaaatgg
    12721 aaaaatcttg accaataaaa tgataaaatc gatcccgcaa aatagaattt tttacattga
    12781 ttaagaaact gaaaaaataa ataaaaaaaa cggaaaaaag actggaagaa tggccagtct
    12841 tttttttcgt ttttgggaag tgaaaaccaa actatttacc tgtaagccac ccataaatcc
    12901 aatcaaatgg tgcaaagaaa aaatcttcca ttctattccc tcctttaaaa aagcataaca
    12961 tgatttctat ttacgtgagc atgaattttg ttacgttttt attgccgaaa aataaaaacc
    13021 gttcattatg atgtcaaatt cgtcagtaat tgatgcaatg tgttataata attcagttaa
    13081 atcaaaaatg aaatggagaa aagacatgga cgggttatta cctttatgga aagaacgcgg
    13141 aatgacaagt catgattgcg tattcaaatt aagaaaaatc ttacatacga aaaaaatcgg
    13201 acatgggggt acgcttgacc cagacgtaga cggtgtgttg cccatttgta ttggaaaagc
    13261 cacaaaagtt atcgaatatt tgacagattc tgggaaaatc tatgtaggcg agataactct
    13321 aggtttcagt acaacgacgg aagatcgatc cggagaacgg gtagaaacaa aaccagtaga
    13381 tatgtcttta tctgaaaaaa aaatagatga agtgatggct tcatttattg gtgagatcac
    13441 ccagatacca ccaatgtact cagcggtaaa agtcaacggt aagcggttat atgaatatgc
    13501 ccgcaataat gaaacagtgg agcgtcctgt tagaaaggct cagatttacc gttttatccg
    13561 gacaagtgaa ttgacaagag acgaagtgac aggaaccatt tcttggcgct tcgaagtaga
    13621 atgcgggaaa ggaacctatg tccgcacatt agctgtagat acaggcaaga aacttggtta
    13681 ccctgcgcac atgtctgatc tgacccgaac agcaagtgcc ggtatgtccc agaaggatgc
    13741 tctgacatta gaagaagtag ctcaagctgt gaaaaatggg acaatcgatg attgtttgct
    13801 gccaatagaa aaaggagtgg aacggttcaa acgagtagat attactgatg aagtatggca
    13861 gaaagtaaaa aacggtatga gacttgatta tactgtattt gcgctcccag aaatgccgtc
    13921 agaagaaatt gctttgtttt ataaaggaaa agttgtcagt atttatcagc caaacccgaa
    13981 agaaaaaaac aaattaaaac ctagtaaagt attgagaaat gaggaataac gaatgaatat
    14041 catcaagatc agacatcctt accaatcaga acaaattcca gaagacgatg tcgttttagt
    14101 gctgggattt ttcgacggcg tgcatatcgg acaccagaaa gtcataaaga caggtatgga
    14161 aattgcacaa aaagaaggat tgaaattagc attgatgact ttcaatcagc atccatctat
    14221 tgtctttaaa aagatcgatc ctagcagcgt gaaatatttg actagcctta agcaaaaaga
    14281 agaaaaaatg gccgctttgg gtatagatta tttatatgaa gttgatttta cttcttcttt
    14341 tgctcattta gcaccgcagg aatttgttga tcaatacatc gtagggttac atgcaaaata
    14401 tgctgtttcc ggttttgatt atacgtatgg accaaaagac attgcagatg tcgctcattt
    14461 tcctgaatat gccaaaggaa gattcaaaat cgtcacagtt tctaaagaag aagaacaagg
    14521 tgaaaaaatc agctctaccc gtattcgtgg attactagac agcggacaag taaaagaagc
    14581 agcaaaacta ttaggttgca catatgaggt agaaggggta gttgtccatg gcgatgcacg
    14641 aggcagacta ctaggattcc ctacagcaaa tgtcaaagta aagagcacgg ttcatttgcc
    14701 taaagaaggt gtttatgtgt cggaaataaa aattggggaa acatggtatc cagccatggg
    14761 ttcaattgga cataatgata cattcggtga aggacgggag ctgactgtag agttgtatat
    14821 tttggacttt aatcaagata tctatggtga acatgtcgcg gtacgctgga atcaattttt
    14881 aagagatcaa gtgaaatttt ctagtgtaga ggaattgatt gaacaactca aggaggatga
    14941 acaagcaaca gctgaatatt tttctcagca ataaaacatt atgcataaaa tctcttgttt
    15001 atgtataact agcaattaca ataaaatggg tgataaataa gcccaggaaa ggggctgtga
    15061 caaaagtctt gtcacagcct ttttttccaa ataaatggtt tttcagaagt agcttcttcg
    15121 gaaataagcc gaaatttctt gaagctgacc acttctgtca caacctcttt ctttatagaa
    15181 ataaaaggac actagcaatt catgaaaacg attgctagag cgtatcattt gctttagact
    15241 atatatcttg gtgtacactc actttaaggc ggaaataaaa gttatccgta ataacaggtt
    15301 taggtatgaa aaaggagtgg gtactatgta caaggaatta gaaaacaaag tcgcagtagt
    15361 aactggtggc tcaaagggga ttggaacagc gattgctcaa cgattcggga aagaaaaaat
    15421 gaatgtagtt gtcaactata actcggataa agaaggagca gaaaaagcag tcaaagcaat
    15481 cgaagaggca ggcggaaaag cagtctctgt acaagcagat gtcagttctg aagaaggaat
    15541 acagcgttta cttgatacgg ctttagaaaa ttttgggaca ctggacgttt gggttaataa
    15601 tgctgggatg gaaaatcagc atccaaccca tgaacttagc cttgaagatt gggaaagagt
    15661 gttaaaagtc aatttgactg gcgtttttat cggaacgaaa gcggctttaa attactttac
    15721 agagaataat aaaaaaggaa atatcatcaa tatgtcttct gttcatgaac agatcccatg
    15781 gccaacgttt gctcattacg ctgcttcaaa aggcggggta aaattattta ccgaaacggt
    15841 agcaatggag tatgcaccga agggaatcag aatcaatagt ataggccctg gtgctatccg
    15901 tacacctatt aatgcggcaa aatttgatga tcctgaacaa aaagaactct tggaaagtat
    15961 gatcccacta ggacgcgtag gtgaaccaaa agaaatagcg gcgacagctg catggttggc
    16021 ttcagacgaa tccagttatg ttacaggtat tacactattt gcagatggtg ggatgacttt
    16081 atatccttct ttccaaggtg gaaaaggata agggtgaata aaaatgggtt tattgattgc
    16141 attgatcccg gcagttggct gggggatcca gccactgatt ttgaaaaaga ttggtggcag
    16201 accaacaaat gagatattag gaactggtat tggagcggtg cttgtcggct tactcgttca
    16261 attattcgct tctccagctg gtatttcagt gactacattt ttgattagtt tactttctgg
    16321 tgcattttgg gttattggac aaacaggaca gtatacatct ttcaacttga ttggtgtttc
    16381 taaaacgatg ccaatttcga ctgcattaca gttagtcgga acctcgttga ttggtgtctt
    16441 cgcttttgga gaatggtctg gaagtatggg gaaaatcatt ggagcagcag caatcattct
    16501 tcttgtattt ggttctgctt taacggcaat cagtgatgat cctggaaaaa aaggcggtat
    16561 cacaaaagga attagtctat tagcatcaac aagtattggt tattgggtat acagtgcatt
    16621 gcctaaacta gttgatgcaa gcggactttc cattttcttt ccgcaaatga tcggtgtctt
    16681 tttaggggca gtgatttatg tgattttccg taaaccaacc gcattttctg aagacaagag
    16741 ttggaaagca acgatcgttg gagccatctt tagtgtgtca gcattggctt atattttttc
    16801 agcaaatgaa aatggggttg ctactgcata tatcattaca cagctaaacg tagttatctc
    16861 tacattaggc ggtatgtaca tcctgcatga aaataagaca ccaaaagaaa aaaaattaac
    16921 gattatcgga ttattgctga tcgtagtagg aagcatggtc acggtcttta tttgaggata
    16981 aagatggtaa aatttcattt attaaaaaaa tatttgtttg aaccaaacag taagtactta
    17041 taaaaaaata gcgctagaat ttccaacatc tctggaattt ctagcgcttt ttaattgttt
    17101 aagatacaag ggaaatatct gtctggatca ataatttttc attgatccat tgactaaagt
    17161 ttacgacata attgaagtta ccaccgatta gtatctccgc atgattgtct ttataaagat
    17221 cgtattgtaa aagacggtct tttttatttg cgtaaacatc gttatggtac tggactaatt
    17281 gttctcggaa ttgattgatt gctgtaaaat cacgatattg aaggttgaaa cgcatcgtga
    17341 ttggtgcatt tagcatgaat cgctgcattt cttttgctgc ttcctctgct tgatctccgt
    17401 ataggaactc aaaaagatta cgttcagaaa atttccattg cccgccaacc tttgttcctt
    17461 ttagttttcc ttcatgaaga taatttctga tagaacgtgt agtaacacct aattgttcgg
    17521 ctaattcatt tactgagtaa aaatctttca atgttcaccc tcctatgtga atggttctac
    17581 tgacttagtt gatatttgct taatcacgaa atgtagaaag aacgatttta attaaggtca
    17641 ttgtaaaaat caagtcagaa aattctaact tttatttctc taaagaaatt cgttctctcc
    17701 taaagtgata ttatatttaa atatacctga aaataatggg cagtattatg aaagatcact
    17761 gatattttct cgtatttttt ccttattcaa aagttttgca ggaaaattgc tgaaaaaata
    17821 attttactat gtagaaaatt caaagccatt caatcagtga tttttttcag cacaagaaag
    17881 actccttata aaaatagcag atactttgtc aattaagtgt ttaaccaggg atgataaaaa
    17941 ggataggatt gaacaaaacc tgtataaggt gatgaatagt ttcctcgcca gtaaaaggtt
    18001 tattcgtata aagctttatc gccaaaattt atcatgacct tttttatctt caatattaaa
    18061 tgaaatcaat tgttcaatca attcccagtt gattacactt ttccaaggaa ctttgatgac
    18121 gttatccgta tatacgtagc ctgcttcttg gatatttttt tcaagggcac gaatcgctgc
    18181 tttttccggt gcgatagaaa aatgattttt agcaaaacta aatgcaataa taaaggtacc
    18241 atggtcagta aacataggtt ggttccattt tacagttgtg tctagctggg gatacgtttc
    18301 tgttatctta gcaaaaaggt ctttgatctt ttgttgatgt tctttttctt tgatttgatt
    18361 gatgatcggc tgaaattttt ccattctaaa acgcctccat taagtggttt gatttatttt
    18421 accagaaaaa caatagttat gtataaaaaa cgaacaaaac acaagtaatg ttgtaaccgc
    18481 tatatttata tgataagatg tctcaaataa taaaatagaa aagagacata aaaatgattg
    18541 acttggcaaa gaaattctta cacgaaaata tatattttga gttgtatcat catcgagcca
    18601 tttatacaaa tgaagatgct ttggtaataa agaaagaaca aggatttaca ggtacagaga
    18661 ccaaaagctt attcttaaaa gataaaaaag ataataacta cgtatatctg acttttacta
    18721 cgaaacaaac ggatttcaaa gcattaggga agatcgtcgg taaaaggctt tctattgttt
    18781 catcagaacg catggaagaa caaacgggac aaaagcaggg cgctgtttct ccgtttggtt
    18841 atgaaaaaca gattccgatt attattgatg aagagctctt atcaaatgaa aaacttgttt
    18901 ttgcaccagg tagaccagat caaacgatgg tcatccaaac ggttgactta aacaaaatca
    18961 tccagctact tgagatgccg atatttttga tgccggcaaa cgaaaaaaac aattaaccaa
    19021 gtaaaatagt gcatacttgg aggtgaagca acatgataag ttatccatta ttagaaagaa
    19081 atgcaacgat tggggttact gcaccatcct caggcgtatc agaggaacac cattcgttac
    19141 tcgaatcagc tttaaagaga atggaagaaa aatctttcca agtagttact ggaaagacgc
    19201 tttggacgca agataaggaa aaatcaggtc cagcaagaaa acgagcagat gaattcaatc
    19261 aaatgatgca agatgaaaat atccaaatga tctttccgac ttggggagga gaattattga
    19321 ttgagattct cgattttgtc gattttgatg ctctacaacc aaaatggata gtaggttatt
    19381 cagatattag tacgttgcta cttgctgtca cattaaaaac agggatagcc actgcacatg
    19441 gaactaacct tattgattta agaggagagt acttggatga aacgacagca atgtgggaaa
    19501 gtgtactgaa aacaagagaa ggagaatcga ttctacagca atcttctgaa aagtatcaaa
    19561 aagtgtggcc ccatgagcat tcatcatctt ctgttttcca tctcactgaa ccaacaaatt
    19621 ggcaatcagt cagcggagat gcagtaagga tgaagggacg attattaggt ggttgtattg
    19681 atacgatcag gcatttaatc ggtactcctt atggagatgt ccaatctttt aaaagaaagt
    19741 ataatgatgg aaagccgatc ttgtggtttc tagaaaactg tgagctatcg tcagttgatt
    19801 taagaagatc acttgttcag atgaaactgg caggctggtt tgatggcagc tcggggatca
    19861 tgttcggtag aagtgcggca gaggatttgt tagattataa aatgaaggat gtatacgaag
    19921 atctgtcaaa ggaattaaaa ctaccgatct tgtatgatat tgattgtggt catgtaccgc
    19981 cacaactgac attgattaat ggtgcctatg cagaagtagc ggtagaagat agaaaagcga
    20041 ctgttttgca aacgtttatt taaaaaattt tttcctgcct tggagtctat caaagaccaa
    20101 taaatctaaa tagaaaagaa agtgaaagaa gctgacaaaa tgctgtcagc ttctttttgt
    20161 ataacatatt agaaagtcta attatctata actgtcacat cgattaactg tttcacgtca
    20221 ttgggatttc ctgtaggaat agctactaca gatgcattat caatgatttc ttgagttgtt
    20281 tctctaatga acgctggata agatgtttga tctgcaggca tttgccaatg tgaaggaact
    20341 ggataactag tgattgtccc gtcatggtta cttgaataca ctaaaactcc ctggtaactg
    20401 tatttcccag tgagcataac cacatctttc ggatagactg cactccctgc atcataaggg
    20461 gcgataggtg ttcctgcagg ataatgaata acatgtaatt cgaaatcacc cgatgtatct
    20521 ctaaaagtat tacctctaat agctagccaa acacgtgcgt actcaatttg tagatcagaa
    20581 tatcctgcta acggatctat tgactgagaa gactgtgagg aggttgtgtc tattgttgaa
    20641 gaagaaatat ttgtctcaac tgtactacta gatgtagaag aagtattggg ctctgagact
    20701 ttactgcttg ttgaagatgt attcgtttca gtagaagctg atttctgagt acttgatttt
    20761 tcagctgaac tggtttgtct gattgaactt ttgtctgtgg tcgcagatgg atcactattt
    20821 tctttactag gagacgtaca tcctacaagg aataaagaga ccaacaataa aataaaacct
    20881 aactttttca tataaaatac ctcattattt ttttaatacc agtatagcat aatcctgaat
    20941 ttttgaatcc gtaaaacctt gtctatgaat ataaatagag tgtatcttct ttgataaatg
    21001 tcagaaaaag tgtgacaaat aatcatcagt aaaattactt gtcacactat tgataaggag
    21061 tatttaattg aagagaatga ttaggagaaa ggatctccgt actttttata gtaatcatag
    21121 aatccaagtt tgtatagacg aatcgttttt tctcttattt cttctgtttc aaaatggact
    21181 gcttgaagaa tcaagtctgt gaattctata ggagctgttc cattcgcagt aatcaaatta
    21241 cgatctttca cagcttgttg ttctataaaa tcttctttat tctgataatt ttccgattct
    21301 aaccaaggaa attgcgcatt tccggtgtgt ttgtaacctt gcaataatcc gctctgagct
    21361 aaataatcaa ctgccccaca aatagcagct actggtacgt ctgtttttaa caatttatcg
    21421 attatttgat ataaatgatg attttttatg tcccatgaat tgccaccgat cataataaaa
    21481 aggtcaactt tttcaggtat ttgttcgatt aagtaatcga tttttgtttt taatcctcca
    21541 atggaagtga cctctgattg gatagaagca gttttgtttt gccatttctc attttggttt
    21601 agtgtgctag taatataagc accttcccaa tcggcatatt cctctaatag aaacgtaatc
    21661 gcagttttca ttttcaagct ctcctttact atctataaca aggacaacag ttttaaccta
    21721 cagacattca ttaaaattgg gtagcgacag cgaaaacaac aattcttaag ttttagacag
    21781 agtatacaga tagtcaactt aattaaatac gaactaaata aaaatgacag gtaatcattt
    21841 ttgtttacca gcattataac attttgcata catggatgac aaaagaacca attcccactt
    21901 gcttgagtac aatattaaaa gaaactggaa ggcaaaaagt aattatagaa acgaaaaata
    21961 aatcatatcg ggcactcaga catgaaaaca accctagaga tatataacta ggtaaccagt
    22021 ttaatgaaag aaaaaatcat ccaagaagtc gattcttgga ttttttagtg gatttgttcg
    22081 ccaaatacgt ttagtatgcg tatatgagag gttgtaagca aagcgttgtg gtctgagcac
    22141 ttaaggagga atctgtgaaa atagcttttc atatttttgc agattttgac ttaatgccga
    22201 aggtcagctt tgcgattacc gtttatcgag aggctgtaaa agcaccgtgt agctccgatg
    22261 acaataagca agcattttta gagctaggtt tccttgattc taaatatgcg gcttatttca
    22321 atgagttggt gcttgaacgc gattttaaga aggtcgaaaa caggcacgac ttttcgacgt
    22381 aaggagaaag aatttcaatg atagaaacaa taaatcccgc agaaaacaag gtttctgcgt
    22441 acattcatat cccattttgc gagcatatct gctattactg tgattttaat aaagtttttt
    22501 tagaagggca gccagtagat gagtatgtgg aaatgctgct gaatgaaatg aaaataatgc
    22561 tagaaagtca tccgacagcg aatttagaaa cactttacgt agggggcggg acaccaactt
    22621 ctttatctgc taaacaactt gatcgactgt tatcaggtgc tagagagatc ctgccttttc
    22681 aaaatggtaa tgagtttact gtagaagcga atccaggtga tttgacccgt gaaaaacttc
    22741 aagtaatgaa aaactatggc gtcaatcgtt tatccatggg agtgcagaca tttgataatc
    22801 gcttattgaa aaaaattgga cgtaaacaca ccgcagaaga tgtgtatcaa actatgcaat
    22861 ttttagaagc agaaaatttt accaatgtaa gcatcgattt gatttatgca ttaccgggac
    22921 aaacactaga aggatatctg gatactttgg aaaaagcttt ggctttggat ctgcctcact
    22981 attctttgta ctcattgatc ctcgaaaaca aaacaatgtt catgaactgg gtacgacaag
    23041 gaagactcga actgcctgat caggaaacgg aaaccagaat gtttgaagag acgattgaag
    23101 caatggaaaa gcatggacgt caccaatatg aaataagtaa ctttgggctc tccggacatg
    23161 aatctcaaca taacctcatg tactggaaca atgaccacta ttttgggttt ggagctggtg
    23221 ctagcggtta tcttggagat attcgttacc gcaacaaagg accaatccaa cactatttac
    23281 gcccattacg agcaaatgaa cgcccggtat tggaagaaga aaaattgaca cgaaaaaatc
    23341 aaatagaaga agaaatgttc ttaggactaa gaaaaaaaac agggattact aaaaaacatt
    23401 tctatgatcg ctatcaaaaa acaattgaat cggtgtacgg tgatgtgcta agggaattag
    23461 agcaagaagg gttacttgtt aatgaagaag accgtgttta tttgacacca agagggacct
    23521 ttttggggaa caatgtgttt gagcgctttt tgtttgaaaa atagtttaaa gaatatttgt
    23581 tttttatctg aatttaaata aaaaaagttc aaaaatacaa taaaggacta ttgaaacgtt
    23641 gatataactg gatttacatc aattatttag aattagcact cttgtgttta gagtgctaaa
    23701 aaatggagtc tttctcttga caggagaagg gcttcttgct atatttataa gtggattagc
    23761 acttagaata tatgagtgct aatgagaggt gaggaagatg ttaacaaagc gacaaaacga
    23821 cattttacgt ctgatcatcc aacactatac gagtagtggc gtgcccgttg gttccaaaac
    23881 attgatgtct gagggagtca aggccagctc agcaacgatt cgcaatgata tgaaagcatt
    23941 agaagatgaa ggattgctgt tgaagactca ctcttcttct gggcggattc catctgcttt
    24001 aggctatcgt tattacgtgg atcatttgtt aaaacctgct cgcgtagcag aagatgattt
    24061 gctgctgatt cgtcagtctc taggcagaga atttcatgaa atcaatgaga ttattaagca
    24121 atctgctgag atattatctg agctgacaag ctatacgact ttttcattgg gacctgaaat
    24181 aaaagaccgc cgactgactg gctttcgtat cgtacccttg aataatcgac agattattgc
    24241 tatcgtcgta acagatatgg ggaatgtaga aagccaagtg ttcactcttc ccgaatatat
    24301 gggaagtcag gaactagaaa aaatggtaca gattgtaaac gaccgtctag tcggagatcc
    24361 tctgatgacg gtgtaccata aacttcgtac ggagattccg atgattcttc ataaatattt
    24421 ccaaacgaca gaagggatgt ctcagttatt tgacaccgtt cttggtcatg tttttgaaga
    24481 taaggtttat gtcagtggac aaatgaatct cttggattat gagccccgtc aagatcttga
    24541 tcagttcaaa tcgatgtttt cttttatgaa agactcagac ggacttacac aaatgatcac
    24601 tccgatggat agcaatattc atatcaagat tggtgctgaa ttaggcaatg atttattgca
    24661 gaatatgagt atgattcaag caagttacga gatagatggt catggaagag gtacaatagc
    24721 cttgttaggg cctactagta tgccttattc tcggatgttc ggtctagtcg atgtttatcg
    24781 aagagaacta gcagctaaac ttgctgatta ctaccgttcg cttgacttgt caaactcgta
    24841 atgtgaataa cgaagctatt tgcagctgat cgttctctct ttcttagtaa gagcaatcaa
    24901 acagctgact gaataatgaa aggaagactg tatcgtgaaa gaaaatcaag aagaactaga
    24961 taaagaaatg acagatgctc agccagaacc ggaaatcgat gtcgaagctg cagaagattc
    25021 gggtatttca gaagctgaag cggaagaatt cgaaacagcg aaattaaaag ctgagttaga
    25081 agaaatggaa gataaatact tgcgggctcg tgctgaaatc gcaaatatgg caaatcgagg
    25141 aaaaaatgag cgtgagcaat tacaaaaata ccgttcacaa gatttagcca aaaaattact
    25201 tccatcaatt gacaacctgg aacgtgcatt agccacagaa gtgtcagatg atcaaggggc
    25261 aggtttgaaa aaaggcgtgg aaatggtctt ggaaagtttg cgtaacgcac tggaagaaga
    25321 aggaattgaa aaaatccctg ctaaaggaga agcctttgat cctaatcttc atcaagcagt
    25381 acaaactgtt ccagcgactg aagatacacc agccgatacg attgtagaag tactacaaga
    25441 aggctacaaa ttacatgatc gcgtgttaag accaaccatg gtcattgttg cacaataatt
    25501 gtgcggatat aaaacttaca ctttcaatta aagaaggaga cgttaattat gagtaaaatt
    25561 atcggtattg acttaggtac gactaactct gcagtagcag tattggaagg aaacgaagca
    25621 aaaatcatcg ctaacccaga aggaaaccgt acaacacctt ctgttgtatc attcaaaaat
    25681 ggagaaatcc aagtagggga agttgcaaaa cgtcaagctg tgacaaaccc aaatacgatt
    25741 tcatcaatca aacgccatat cggtgaagca ggttacaaag tagatgtaga aggtaaatca
    25801 tatacaccac aagaaatctc agcaatgatc cttcaatata tcaaaggatt cgcagaagac
    25861 tatttaggtg aaaaagtcga aaaagcagtt atcactgtgc cagcttactt taatgatgca
    25921 caacgtcaag caacaaaaga tgctggtaag atcgctggtc tagaagtaga acgtatcgtc
    25981 aatgaaccaa ctgcagctgc tcttgcctat ggtttagata aaacagatcg cgacgaaaaa
    26041 atcttagtat tcgaccttgg tggcggtact tttgacgtat ctatccttga actaggagac
    26101 ggtgtctttg atgtattgtc aacagctggc gacaaccatc taggtgggga cgactttgat
    26161 aacaaaatca tcgactacat ggtagcagaa ttcaaaaaag aaaacagcat tgacttgtct
    26221 caagacaaaa tggctttaca acgcttgaaa gacgctgctg aaaaagcgaa aaaagattta
    26281 tctggcgtat caagcacaca aatcagcttg ccatttatca cagcaggaga atctggacca
    26341 ttgcatttgg aaatgacatt gacacgtgca aaatttgatg aattaacagc tgacttagta
    26401 gaacgtacaa aagtaccagt acgtcaagcg ctaaaagatg caggtttatc acaatcagaa
    26461 atcgacgaag ttattttagt cggtggatct actcgtatcc ctgcagttgt agaagcagta
    26521 cgtaaagaaa caggaaaaga accaaacaaa tcagttaacc ctgatgaagt agttgctatg
    26581 ggtgctgcta tccaaggtgg cgtgatcact ggtgatgtga aagatgttgt tttacttgac
    26641 gttacaccat tgtcattagg tatcgaaaca atgggtggag tgttcactaa attgattgac
    26701 cgcaatacaa cgattccaac aagtaaatca caagtattct caactgcggc agataatcaa
    26761 ccagcagtag acatccatgt attacaaggt gaacgcccaa tggctgctga caacaaaact
    26821 ctaggaagat tccaattgac agatattcca gcagctcctc gtggtattcc acaaatcgaa
    26881 gtaacattcg atattgataa aaacgggatt gtaaatgtat ctgcgaaaga tctaggtaca
    26941 caaaaagaac aaaaaatcac gatcaaatct tcttcaggtc taacagacga agaaatcgaa
    27001 cgtatggtaa aagatgcaga agcaaatgca gaagcagata aagctcgtaa agaggaagtt
    27061 gacctacgta atgatgtaga tgcattgcta ttctctgtgg acaaaacatt gaaagaatta
    27121 gaaggcaaag tggacgaaga agaagtgaaa aaagcagaaa cagctcgcga tgaattgaaa
    27181 gccgctgttg aagcaaacaa catcgaagaa atgaaaacta aacgcgatgc attgaacgaa
    27241 atcgttcaaa acttgactgt taaactatac gaacaagcgg cacaacaaca agctcaagaa
    27301 aacccagaag cagctcaagg cggagcagat gatgtcgttg atgctgattt tgaagaagtc
    27361 gatggcgacg ataaataatc agttcactga atttcgagag aaggccaaag ttgctacttt
    27421 ggctttttct tttgaaaaat agtttgaagt atcggttttt gtttcaaaca aaatatgata
    27481 tgataacaag cgacgtgaaa atgatgagaa ccaataggag gaaagcctat ggcaacaaaa
    27541 cgtgattatt atgaagtctt agggctctca aaaggagcat cagaagatga aataaagaaa
    27601 gcttatcgta agctttcgaa aaaataccat cctgacatca ataaagaacc tgatgcggaa
    27661 gaaaaattca aagaagtatc agaagcatat gagattttaa gtgacccgca aaaacgcgct
    27721 gcatatgacc aatacgggca tgcaggcaca gatccaaatt acggtgctgg tggagctggt
    27781 ggcggatttg gtggattcgg cggcttctct ggtggcggct tcggtggttt tgaagatatc
    27841 ttcgactctt tctttggagg aggcggtcgg tcagtcgatc caaatgcgcc tcgtcaaggt
    27901 gctgatttgc aatatacggt cgacttgtct tttgaagaag cgatttttgg tgtagaaaaa
    27961 gaaatcaagt ataatcggga agaaatctgt catacttgtg gtgggaacgg tgcaaaacca
    28021 ggcacacaac caactacttg tcataaatgt catggtgcag gtacgattaa tgtagaacgc
    28081 caaacaccac ttggacgagt gatgagccgt caaacttgtg atgtttgtca tgggacaggg
    28141 aaagaaatca aagaaccttg tccaacctgt catggctcag gacatgagaa gaaagcacat
    28201 tcagttaaag tgaatgtacc agctggtgta gaagaaggac aacaaatgcg tttagctggt
    28261 caaggagaag ctggagaaaa cggcggacca tttggtgatt tgtatgtggt cttccgtgta
    28321 gaagaaagtg atatctttga tcgtgaaggt tctgaaattt actatgaatt gcctttaagt
    28381 tttgtacaag cagctttggg ggatgaagtc caagtaccaa ctgtccatgg agatgtgaaa
    28441 ttgaagatcc ctgctggaac acagacaggt acgaacttcc gtttacgtgg aaaaggcgct
    28501 ccgaagttac gtggtggtgc aacaggcgac caacatgtca aagtaaaact gatcacaccg
    28561 aaaaatctaa atgaacaaca aagagaagca ttacgtgcat tcgctgaagc tggcggcatc
    28621 aaagttgaag aacagcaaga agacggtttc ttcgataagt ttaaagatgc ttttggcggg
    28681 aaaaagcgga agtagttttt ctagtaaaaa cgctaagtca ttaataatta aaaaccagac
    28741 gaacaatcat atgttcgcct ggtttttttg tagttgtcat gtttgtacca acaaaaaagc
    28801 cagctttaga atatgataat cgtcatagtt tctaggggta taaccagtga gagaatggaa
    28861 cttgttcaat cgatattgca aggtgttttt atgaataaat aattcttctg agcaattttt
    28921 gatgcttccg ttatttttac tgaagcatgc caaagtcttc ttgaacaaat gtacttcttc
    28981 ttctgtcagg cttttcaaaa tacgttttac atataatgtt cgttgatctg acgggattgc
    29041 tggtaaaacg atcccgaggt ctaaatcttc aaaaaagcgg atctcgttca cagattgatg
    29101 gaaagctatc cattcgcaaa cttgtttggc atgaaggaaa gattgcggtg tctcttgctc
    29161 tgtataacaa agcgacccaa ttccaaagtt tagctgtaac tgatacattt tattaaactt
    29221 tgtctggatt ttatcaagca atgatagcaa tgaggctctg gaaatccgat tgactaggat
    29281 aatgatttgt tgtctatgta cagtgagcaa agcctgtccg taataagaga agaccctttc
    29341 gagcaactcc aataaaggat tgatccgttc ttgacacaat tgctgattgt cagaaagttg
    29401 tcccacaact acataatatg gagaagaaaa atcaagctca attaagtgag ggtggtatcg
    29461 actatgtact tgatatcatc aatgaaccga tggtactttc ttatgcgatg gaacaggtag
    29521 agcgattgct tgtttgtgca ggaaaacaag cgatgcggct gatacgatta ggtgagaaaa
    29581 taagaagata aaaatacacg gtcccttcat ggcgaccgtg catttttggt ttttattaaa
    29641 tatttaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt tcaaagattt
    29701 gttgaggcgt tccatcttct aaaaagtttc cgtcgtcaat gaacatgacg cgattggcca
    29761 cttctttagc gaaccccatt tcgtgcgtaa cgataaccat cgtcatacct tgttctgcta
    29821 actttttcat gacgttcaag acatcaccga ccatttcagg atcgagtgca gaagtaggct
    29881 catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc acacgctgtt
    29941 tttgtccacc agaaagcatt tcaggataca tatctttttt atcttctaga ccaactgtct
    30001 gcagtaattt gattgctttt ttctctgctt cttctctaga ttctttcttc aggtccatcg
    30061 gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag tgttggaata
    30121 ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc aagtgggcgc
    30181 cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa cgcaagaatg
    30241 tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg ttgatggata
    30301 cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc tcaactaaga
    30361 ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt tgtcaatgct
    30421 gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact ttgtgttgta
    30481 cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc ggaaatgatc
    30541 gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat gatgcgtatc
    30601 gcttgcggca aaatgatttt ttgcattgtc cgattataag aaagacctaa acttctggaa
    30661 gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc tgcgatataa
    30721 gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat cgtaaatcca
    30781 acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg aattcctcgg
    30841 atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc tacactgaat
    30901 aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt taggagaatt
    30961 gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc aagaaatgtt
    31021 gattcatcag cagtggaaga ctcactggaa gtttctgaac catccgctac atattcttta
    31081 ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt gaacatatta
    31141 agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc agattctttt
    31201 ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac cggataatca
    31261 tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc agcagcatca
    31321 aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc agcactttcg
    31381 gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt gatcgaagta
    31441 tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga aaaatcatag
    31501 cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc ttgtcctgat
    31561 tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt aaatccttgc
    31621 agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc ctttgcgttt
    31681 tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc ttttttgggc
    31741 tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt gtcagcttgt
    31801 gcaacatgac caaacgcaaa tgtcacaagt cctgtcatta agaaaacaaa tgagtgaaat
    31861 aaatgctttc ttttcataat caacctccta aatttccaat attttattat tataggcgaa
    31921 aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg agtttctaac
    31981 attttatgtt ataaaacata acatgtataa taagataaaa aagtggcgaa agtatgttcg
    32041 gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt attggtcgta
    32101 aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat acctcccgac
    32161 actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg attaaccaat
    32221 tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg actggaacag
    32281 ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca ctgattattg
    32341 cccataacaa aacattggct ggtcagctgt acggggaatt caaggaattt ttccctaaca
    32401 atgctgttga atattttgtt tcttattatg attattatca gcctgaagct tatgtgcctt
    32461 caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac aaattacgac
    32521 attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca tccgtttcat
    32581 gtatttttgg attaggctcg ccttttgagt accaaaaaca agtagtttcg attcgacagg
    32641 gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa ttcgaaagaa
    32701 atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg gagatttttc
    32761 cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag gtcgaacgta
    32821 tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac gtagcaatct
    32881 tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta gcgaatatca
    32941 aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt ctggaagctc
    33001 aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg ggctacacgt
    33061 cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa ccgccttata
    33121 cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg cacgtaacca
    33181 tgcctcaaat cagaggaatg tataatggtg accgagcgag aaaacagatg ctagtagatt
    33241 acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa gagtttgaaa
    33301 aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa catgaacaaa
    33361 cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca gtgatcgaag
    33421 ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa cgagtcgaaa
    33481 aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac ctcacagatt
    33541 atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa acgctggaac
    33601 gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt ggaatcaatt
    33661 tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg gatgcggata
    33721 aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga gctgctcgga
    33781 atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg cgtctagcga
    33841 tggatgaaac gtcaagacgt cgtacgattc agcagaagta caatgaagaa catgggatcg
    33901 ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa gaatcggaag
    33961 atgatacaaa agaagcggta caagtctcgt acgaagaaat gacgaaggaa gaaaaagaca
    34021 cgttattgat gaaattagaa aaagaaatga aagatgcggc aaaagctttg gattttgaaa
    34081 cagccgcgaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag gaggacaaca
    34141 tggctaacga taaaattacg attcatggtg cacgtgccca taacttgaaa aatatagatg
    34201 taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct ggaaaaagtt
    34261 ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag agcctttctg
    34321 cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc atcgacggat
    34381 tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg tcgacagtag
    34441 gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta ggacatccga
    34501 tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg gcagatcaag
    34561 taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg gtaaagaaaa
    34621 aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc cgcgttcgag
    34681 tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac aaaaaacatg
    34741 atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct cgcctatttg
    34801 attctttaga ggcggccctg cgattagcag acggctatgc tctggttgac gtgatcggtg
    34861 aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt actgttggcg
    34921 aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca gactgtgatg
    34981 gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga acgaagacac
    35041 ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat cctcaaatgt
    35101 tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa gaattaccgg
    35161 aagagcatca ggagatcatc ttgaatggga cagatgatct tttccatttc cattatgaaa
    35221 atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg aagaatatcg
    35281 agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt ctttatatga
    35341 cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct ctatctgttc
    35401 aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa gcagtagatt
    35461 tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg attttaaaag
    35521 aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta actttgagtc
    35581 gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct acacaaattg
    35641 gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga ttgcatcaac
    35701 gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc aacaccctcg
    35761 ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatttgatt gacgtcggac
    35821 ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa gaagtagcta
    35881 atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc cctgtaccaa
    35941 aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca gaaaacaatc
    36001 tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca ggtgtgtctg
    36061 gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg caaaaattaa
    36121 ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa agtatcgaaa
    36181 agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat ccagcgactt
    36241 atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag gcaaaaatgc
    36301 ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt gaagcttgtc
    36361 gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat gttccttgtg
    36421 aagtatgtca tggtaaacga tataattctg aaacattaga agtgcattac aaaggaaaaa
    36481 gcattgctga tattttggaa atgacagtag aggatgctgt agaattcttc aagcacattc
    36541 caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat gtgacgatgg
    36601 ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt gccagtgaat
    36661 tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg acaggacttc
    36721 atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat gctggtaaca
    36781 cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat atcattgatt
    36841 taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca ccagaagaaa
    36901 tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg gtataaagag
    36961 gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt gttttattaa
    37021 tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt atagataaaa
    37081 acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggac tttcctttga
    37141 ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa acaagagatt
    37201 atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat ccctatatta
    37261 ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc atacggcaaa
    37321 atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa aattgtcaat
    37381 gtagaagtta ttccaggatt tgataaagac cctgtattta tcgagacgat gcttcgtttg
    37441 gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg tggtgatgga
    37501 tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat ctgtccatat
    37561 attgattatt ctttatttga aaggctgatc aataaagtaa gcttttatga agtttgtgaa
    37621 gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt agtaaatgga
    37681 catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc aaacagtgtg
    37741 gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga gactagagaa
    37801 gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga aatgattgta
    37861 caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta tgtagatgaa
    37921 gatcatcaag ttcggatgat gtgccttggt catccactat tagaagatcc tacaccagca
    37981 tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta tcaaaccatt
    38041 aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat gaagtatgat
    38101 cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg tagcagtttc
    38161 tttgtgacat tgaacggctt gaatctggct cgttttgtca cagaagatcg agtgttcaac
    38221 aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag attatggatg
    38281 ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa aaaactagct
    38341 gagcaaatga tcaaagaaaa tcgttacggt actactgtat tttatgaaaa agaccgatca
    38401 atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag attcaaaaag
    38461 tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt aggcggaatg
    38521 ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa agctacaaaa
    38581 gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga tcgtacagct
    38641 tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga tattgagaaa
    38701 caaaatttat tgcgaccaaa cttcattgtg ttgacttgta acaccgcaca ctactttttt
    38761 gaagagttac aagcggcaac ggatattcct atcttgcata tgccaagaga agcagcaaat
    38821 gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga aggaagtatg
    38881 aaagctggaa tctatgaacg cgaagtcaaa aatcttggtt tcgaaacagt gattcctgat
    38941 actgcgcttc aagaaaaaat caattacctg atctatcatg agatcaagga atcggattat
    39001 ttgaatcaag agctctatta cgaaatctta gaggaagcgg tagagcgcct aaactgtgaa
    39061 aaagtcatcc taggttgcac agaattatct ttgatgcatg aatttgctga agataatcat
    39121 tacccagtga tcgatgctca atctatttta gcggatcgaa cgatcgaacg tgcattagct
    39181 gaaagaagcg aggcattgga tacagcatcg gaaaagtaaa aaaattggat ttggggcaaa
    39241 tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac tggtaatgat
    39301 acgcccggga tgatataatt ttattatgca caatgtgaga ggggttcgta aatatgacag
    39361 ataatttgca attagtcatc ataacaggta tgagtggtgc agggaaaaca gtagctatcc
    39421 aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga ttgattccaa
    39481 aattctggga attgatcaaa gaatcaggta aagtcacaaa aatggcttta ggagtggatt
    39541 tgcgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc gaaaatacga
    39601 acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta gtggctcgct
    39661 acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact gaagggatac
    39721 gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc attgatacga
    39781 caacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa tcaaagggag
    39841 accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg ccgatcgatt
    39901 cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg gaactaagac
    39961 cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat gaaacgcaat
    40021 cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac attaaagaag
    40081 gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt tctgtggcat
    40141 tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg acacatcggg
    40201 acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct accggataag
    40261 aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc tgaaaagttt
    40321 acgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg gtggaagcag
    40381 cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa atgtcttagt
    40441 ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt tcaaaaagga
    40501 agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag tttccgagat
    40561 gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg tagatggaca
    40621 tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtttttaaag acggaacgac
    40681 tgcagtagga gaatctaaaa tcgcgatcaa ccgtaaaacc attgaccgtg tgtatgtctg
    40741 caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag caattatgga
    40801 cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc caaatttagt
    40861 gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct atatctgtaa
    40921 tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata tacgtgtttt
    40981 acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg aaaaagtgcc
    41041 tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg cacacgactt
    41101 caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttag aattgaaaga
    41161 tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt tagttttcgg
    41221 ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt catttgctgc
    41281 agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga aagcggaatt
    41341 agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt ttattttgaa
    41401 cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga aagatcatta
    41461 tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga acaatgtcta
    41521 tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata tcatggatgg
    41581 tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa aaatgcgttc
    41641 ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa cgagtcgtta
    41701 tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg atatgttaaa
    41761 ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt cgtatttaaa
    41821 aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact caatgctccg
    41881 atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc tagtcaattg
    41941 tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg aaaacatcga
    42001 attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg aaatcgcaga
    42061 acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga ttccctcagg
    42121 tgcaatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat ttgcggaaaa
    42181 actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca ggagatgcga
    42241 atgtatcagt tttttttaaa agctatactt gcttttcttc cgtcaaacta taattccaac
    42301 tttactggag taacataatg ttgaaaggct tctattgttt tttaatacaa tcgtataaaa
    42361 agcggggaga caactgaatg aagattttag ttttatacat aaacttttgc ttaatataga
    42421 agtcctgata tagataggat caagtattta tctataactg gagattaatt aaaaactgga
    42481 aggaactgct cgatcaacct tgagaactgt ttttcttact tctggtcaag attctcgtgg
    42541 ttggtggata aggatggttg ataagttcat tggattagct agtcgaaaac aaccaggtgt
    42601 aattaatgta gaagttttaa caaggggatg gagtgattta ttcagtgata atcataagtt
    42661 cataaatagt aattgggatg tatggttggt atacgttata cctgaacttg aactatcaag
    42721 atttggttta gaatttgaga atcgatgttt ataaagggag ggcaaaaaat gtgctttttt
    42781 acaactttta ctatcattat gattgcagta ctttttattt ttctagatat agcaaagcga
    42841 aatacagcat ttttattata tcgggtttta cttcgagcag gtctcataac atttatatcg
    42901 atcgttggat tttttctatt cacagttatt gtatttattt ggagaactac agcaccacca
    42961 ttgccagaga ttacatatgg agaatttccg tttagtcttg aatatgaaat agatgaggaa
    43021 agaattttga ttgaagatac ggtgattgca gaatttatag gaagttttag agggaatgtg
    43081 acaactcaac ctagacgaag ttggagtata aaacttttaa ataatagtgg agagttgaaa
    43141 gcatttcatt atgaattctt aataatagaa agtgaagatg taacaatcac tttaagtagt
    43201 ggctctgctg gatattttat gaatgatggt ggaagcaagc cacagctcac agtccgtgat
    43261 cgtattgcag gttttatgca tttttctcac tatgacttag aagaagctcg taaaatactt
    43321 gcagaatatg ggatcacatt aatcaattgg gaatatgcgc caccgataag aaattttttc
    43381 aatgattatt attagtctta taaaaaacag aatgtaaaat ttatgtgcgc gagtatttag
    43441 ttttccattt tttgtttgat agatatatag cattgttgaa gcatgggata gtggtgccaa
    43501 ttttatcaat gaggttggtc aggcagttaa cataattggt gatgctataa atccatttaa
    43561 ttggaattgg aggaattagg tgtggaaaca gtgagattat ggatattcat gaaaaatttt
    43621 cgttcggctt tgagatatgt tattctggaa tcaaaatcag gttattatct gattgacaca
    43681 gatcactata ctttttgggg aggctacctt tttccgattt ttaattggat aatcccccat
    43741 aaggttatga aaattaactt gcctgcagaa gaaataaata gattaatact caacaaagat
    43801 ttacaaagga aaagagagaa agaacattct aatgttgctt ggataggtcc agcatttgca
    43861 gtagtgactt cttcaattgt cggtcctatg ctagagagta tttatagtga tttttctatt
    43921 ggaatacgtt atttatttgt tactttaagt atagctgttc ttttgtgcat aaagggttat
    43981 gttagcaaga agaataaaga tattttaaca ctaatcggtg taaacaattt atctagtact
    44041 aaaattatac tgtttcctga atctataaaa gaaggatcaa ggttgttgat aatttttata
    44101 atgcttatac tgttttctgt tttatttggg gtgatgttta taga
//
LOCUS       DAZKAS010000007        43749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000007 DAZKAS010000000
VERSION     DAZKAS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.007"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..458)
                     /locus_tag="ACG8H7_000290"
     CDS             complement(114..458)
                     /locus_tag="ACG8H7_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP8982427.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(451..885)
                     /locus_tag="ACG8H7_000291"
     CDS             complement(451..885)
                     /locus_tag="ACG8H7_000291"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFP8982428.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1023..1760
                     /locus_tag="ACG8H7_000292"
     CDS             1023..1760
                     /locus_tag="ACG8H7_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982429.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGDIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1753..2964
                     /locus_tag="ACG8H7_000293"
     CDS             1753..2964
                     /locus_tag="ACG8H7_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982430.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3168..3950
                     /locus_tag="ACG8H7_000294"
     CDS             3168..3950
                     /locus_tag="ACG8H7_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFP8982431.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4020..4682
                     /gene="trmB"
                     /locus_tag="ACG8H7_000295"
     CDS             4020..4682
                     /gene="trmB"
                     /locus_tag="ACG8H7_000295"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP8982432.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4780..6534)
                     /gene="ade"
                     /locus_tag="ACG8H7_000296"
     CDS             complement(4780..6534)
                     /gene="ade"
                     /locus_tag="ACG8H7_000296"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP8982433.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6666..7595
                     /gene="comGA"
                     /locus_tag="ACG8H7_000297"
     CDS             6666..7595
                     /gene="comGA"
                     /locus_tag="ACG8H7_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFP8982434.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7552..8601
                     /gene="comGB"
                     /locus_tag="ACG8H7_000298"
     CDS             7552..8601
                     /gene="comGB"
                     /locus_tag="ACG8H7_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFP8982435.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8598..8876
                     /gene="comGC"
                     /locus_tag="ACG8H7_000299"
     CDS             8598..8876
                     /gene="comGC"
                     /locus_tag="ACG8H7_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFP8982436.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8851..9321
                     /gene="comGD"
                     /locus_tag="ACG8H7_000300"
     CDS             8851..9321
                     /gene="comGD"
                     /locus_tag="ACG8H7_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFP8982437.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9293..9640
                     /locus_tag="ACG8H7_000301"
     CDS             9293..9640
                     /locus_tag="ACG8H7_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982438.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            9603..10028
                     /gene="comGF"
                     /locus_tag="ACG8H7_000302"
     CDS             9603..10028
                     /gene="comGF"
                     /locus_tag="ACG8H7_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFP8982439.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            10000..10359
                     /gene="comGG"
                     /locus_tag="ACG8H7_000303"
     CDS             10000..10359
                     /gene="comGG"
                     /locus_tag="ACG8H7_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFP8982440.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10531..11538
                     /locus_tag="ACG8H7_000304"
     CDS             10531..11538
                     /locus_tag="ACG8H7_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8982441.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11563..12747
                     /locus_tag="ACG8H7_000305"
     CDS             11563..12747
                     /locus_tag="ACG8H7_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFP8982442.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13184..14767)
                     /gene="abc-f"
                     /locus_tag="ACG8H7_000306"
     CDS             complement(13184..14767)
                     /gene="abc-f"
                     /locus_tag="ACG8H7_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFP8982443.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(14872..15000)
                     /locus_tag="ACG8H7_000307"
     CDS             complement(14872..15000)
                     /locus_tag="ACG8H7_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982444.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(15345..15488)
                     /locus_tag="ACG8H7_000308"
     CDS             complement(15345..15488)
                     /locus_tag="ACG8H7_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP8982445.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15710..16189)
                     /locus_tag="ACG8H7_000309"
     CDS             complement(15710..16189)
                     /locus_tag="ACG8H7_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP8982446.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16262..16546)
                     /locus_tag="ACG8H7_000310"
     CDS             complement(16262..16546)
                     /locus_tag="ACG8H7_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982447.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16624..16818)
                     /gene="ssrS"
                     /locus_tag="ACG8H7_000311"
     ncRNA           complement(16624..16818)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8H7_000311"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16890..18176)
                     /locus_tag="ACG8H7_000312"
     CDS             complement(16890..18176)
                     /locus_tag="ACG8H7_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFP8982448.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18314..18985
                     /locus_tag="ACG8H7_000313"
     CDS             18314..18985
                     /locus_tag="ACG8H7_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFP8982449.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18951..19439
                     /locus_tag="ACG8H7_000314"
     CDS             18951..19439
                     /locus_tag="ACG8H7_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFP8982450.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19452..20399
                     /gene="prmA"
                     /locus_tag="ACG8H7_000315"
     CDS             19452..20399
                     /gene="prmA"
                     /locus_tag="ACG8H7_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP8982451.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20400..21164
                     /locus_tag="ACG8H7_000316"
     CDS             20400..21164
                     /locus_tag="ACG8H7_000316"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP8982452.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21397..23610
                     /locus_tag="ACG8H7_000317"
     CDS             21397..23610
                     /locus_tag="ACG8H7_000317"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP8982453.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23621..24067
                     /gene="dtd"
                     /locus_tag="ACG8H7_000318"
     CDS             23621..24067
                     /gene="dtd"
                     /locus_tag="ACG8H7_000318"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP8982454.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24238..25497
                     /locus_tag="ACG8H7_000319"
     CDS             24238..25497
                     /locus_tag="ACG8H7_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFP8982455.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25586..26533)
                     /locus_tag="ACG8H7_000320"
     CDS             complement(25586..26533)
                     /locus_tag="ACG8H7_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP8982456.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26927..28219
                     /gene="hisS"
                     /locus_tag="ACG8H7_000321"
     CDS             26927..28219
                     /gene="hisS"
                     /locus_tag="ACG8H7_000321"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP8982457.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28234..30003
                     /gene="aspS"
                     /locus_tag="ACG8H7_000322"
     CDS             28234..30003
                     /gene="aspS"
                     /locus_tag="ACG8H7_000322"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP8982458.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30112..30987
                     /locus_tag="ACG8H7_000323"
     CDS             30112..30987
                     /locus_tag="ACG8H7_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8982459.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31253..33136
                     /locus_tag="ACG8H7_000324"
     CDS             31253..33136
                     /locus_tag="ACG8H7_000324"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP8982460.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33211..33495)
                     /locus_tag="ACG8H7_000325"
     CDS             complement(33211..33495)
                     /locus_tag="ACG8H7_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP8982461.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33485..34219)
                     /locus_tag="ACG8H7_000326"
     CDS             complement(33485..34219)
                     /locus_tag="ACG8H7_000326"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFP8982462.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34348..34971
                     /locus_tag="ACG8H7_000327"
     CDS             34348..34971
                     /locus_tag="ACG8H7_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFP8982463.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35163..36284
                     /locus_tag="ACG8H7_000328"
     CDS             35163..36284
                     /locus_tag="ACG8H7_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP8982464.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36291..39419
                     /locus_tag="ACG8H7_000329"
     CDS             36291..39419
                     /locus_tag="ACG8H7_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFP8982465.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39438..42632
                     /locus_tag="ACG8H7_000330"
     CDS             39438..42632
                     /locus_tag="ACG8H7_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFP8982466.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42778..43485)
                     /locus_tag="ACG8H7_000331"
     CDS             complement(42778..43485)
                     /locus_tag="ACG8H7_000331"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8982467.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14117 a         7765 c         9237 g        12630 t
ORIGIN      
        1 taaaaacgtt gtcgtggtag tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa
       61 tacgcaaact tttctcactt atatagttaa atctgttgag aaaataatca ttattatatt
      121 tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt gatttgtgtg
      181 atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc ttcttggaaa
      241 gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt aaaatgattc
      301 aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc gatcagtttc
      361 tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt gccagcagca
      421 gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct gctttacgat
      481 tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat tgacaaagtg
      541 aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg aatgaaaaac
      601 agattgatag gctagttcac ggttgttatt caagatatta agacctttca tttctggaaa
      661 agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc ctgcaagttc
      721 gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg tgtgtccttt
      781 cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata ttttatagct
      841 tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa acatcctttc
      901 tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg taaggaaaaa
      961 aacaaaatac cgtcattttc ttcgtggtat aataaataaa aataaatggc ggaggaaaaa
     1021 gaatgagttt aattatcgat catgtcacag gggggtacgg acatctccct gtgttaaaag
     1081 atatcagttt cgatgtacag cctggagaga tagtagggtt gattggactg aacggagcag
     1141 ggaaaagtac gaccatcaag cacattatcg gtctgctgga agcttcaaaa ggaacgatag
     1201 aaatcaatca tctatcctta aagaaaaata cggaagaata tcgcaaacaa attggggata
     1261 ttccagagac tccttcttta tatgaagaat tgacattgag agaacatatc gaagtcactg
     1321 cgatggccta cgatatacct atggatatag caatgaaaag agcagaaaag ttgttggaga
     1381 cttttcggtt atctaatcgt cttgactggt tcccagctaa tttttcaaag ggaatgaaac
     1441 agaaagtgat gatcttatgc gcctttttga tcgagccttc tttatatatc atcgacgaac
     1501 catttttagg cttggatcca ctagctatca atgcattgct tgaattgatg gtagaaatga
     1561 aaaaagaagg agccggtatt ttgatgtcta cgcatatttt agctactgca gaaaaatatt
     1621 gtgatcgttt tgtggtctta cataacggag agatacgagc agacggttct cttgagatgt
     1681 tgagaaaaga atttcagctg ccggattctt ctttggacga aatctatatt gctttaacaa
     1741 aggagcaggc acatgagtag tttttttcaa aaacgtttag caagacatca aaagaaaatg
     1801 atgaaatata tgcgttatgt attgaacgat cattttgttt tagtttgtct tttcttgatt
     1861 ggcggtgtgg gattatatta ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt
     1921 ggagggctca taatctctgt attttggatg ctctgtttat tcgtgggtaa gatggctact
     1981 tttgcagagg cagcagatat tgttttttta cttcctaaag aaaaagaaat gcaaacctat
     2041 ttggaacgag cttttcgcta ttcttgcatt tttccgttga tctttttgat attggcatgc
     2101 ggattggcca tgccattagt ggtggttagt acggagcaat ccttttcgat gtttttcgtc
     2161 tatctgatca tattatggag tttgaaattc agtcatctgc aaatcaagag agcagaattg
     2221 tttcgactag atcaacaagt tattcgttat tggaaattag gctggttttt ttcttctctg
     2281 atctttttgt ttctatcatt ttattggcta atatgggcag gaatagcagg tgcattagta
     2341 caggcaagtc tatattactg gtttttgtgg aagcaacaag aaaaaggatt ggattgggag
     2401 aaaatggtgc atgtagaaga acataggttg catcagatct atcaatttat caatttattc
     2461 acagatgttc ctgaaattac ggcaaagatc aaaagaagac gctatttgga tcctatattg
     2521 actcatatta aaagagaatc aaaaaacact tatctttact tgtttgcaag aagattggca
     2581 agaggttctg attttagtgg tctttatttc cgattagtaa ttataggtgg gttattgatt
     2641 gccggagtag tcgactttta ttttagtctg attatcggtt tcttattttt atatttgatc
     2701 ggtttccaac tgttaccttt agcgaaccag ttccgctata tggttctcgt tcagctttat
     2761 ccaatccata agaatcagaa gaagcaggcg attcagtatc tactcacatg ggtattaatt
     2821 atcgcgtcgg ttatttttgg tttgattgct gcagtcgtat tagatgggca gcagcgatgg
     2881 gttccgctag cggcttattt gattgttact atgctctttg ttaaactata tgtaccaact
     2941 cgtttgaaga agatgtcaga ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa
     3001 atagagcgtt gacagtaaga atgctgttcg ttctattttt tttgagaaaa gctggttttt
     3061 tgaatggcaa ttaaatgaaa aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact
     3121 agaataataa gtactgcaaa acaaagagta agaggacggg agaatgcatg atggagtttc
     3181 agttagacca agagtggcgg ttacgtccca tcaaaggcga cacaggaaaa acatacatag
     3241 gcatgcgtga tgaggacaaa gtctttatta aacgaaacac gactccgatg ttggctgctc
     3301 tttcaaggga aggaattaca ccaaaacttg tttggacaaa acgaacgggg aatggcgata
     3361 ctttgacagc acaagagtgg ctggaaggtc gcttgctgac tcctaatgag attggtcaaa
     3421 gaaacgacgt gattgatgtt ttgtaccatt tacatcattc aaattctctt aaaaccatgc
     3481 tgacccgtat cggtggtcgt gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag
     3541 aattaccgga agaactccag aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg
     3601 agcagatccc gtcaatagat aaggaaaact tatcagtagt acacggagat gtaaaccatc
     3661 gtaattggtt agtatgtaaa aattatttat atctagtcga ttgggattca atcatgtttg
     3721 cagaccctgc ggtggatatt gcaacgattt taggaaatta tgtgccactg tctagctgga
     3781 atcagtggct gatcagttat ggcatacgcc cgactgacga aatgcttgaa aaatttcatt
     3841 ggtatgcggc catgaatttg ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc
     3901 gacggatgaa cgaagaaatt ttacaattaa aacgaatttt cagtggctga ggtttggccc
     3961 aaactctgcg gagatttggc cagacctttt taattagagg aaaggaatgg agataaataa
     4021 tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg aattttgtaa
     4081 ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa
     4141 tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca aaagcccatc
     4201 ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca
     4261 agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga tcagcgttga
     4321 cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc gatccatggc
     4381 caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct gtagatgaac
     4441 agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga ttgtttgagt
     4501 attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg ttagatttac
     4561 atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt tcttcaaaag
     4621 gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt
     4681 gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatct cagaaaatga
     4741 acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat ttatttcatc
     4801 ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt
     4861 gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa
     4921 ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact gttcatagac
     4981 gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac aagctatttc
     5041 ctgctgtttg ttgacaacgg tcatcccgcc acctttttga atcaaaagat tagcagcata
     5101 cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat gggcaatcgt
     5161 tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc caatgtttcc
     5221 cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga ttgaaggaat
     5281 gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta gactattcgg
     5341 gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg aatgtggatt
     5401 taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat gatcagcaaa
     5461 taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga aaacttcatg
     5521 aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc caggggcaat
     5581 cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca tctgaatggc
     5641 ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga ttgatccttc
     5701 ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc ttgaattttc
     5761 aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt ctcgaaccat
     5821 taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag cttcatgatc
     5881 cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac cagctgcatg
     5941 cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga tgtctgtttc
     6001 attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt cgggtttttg
     6061 taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa aaggggttac
     6121 aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg catcttctat
     6181 cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat ctgtcactac
     6241 agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata aaagtgagct
     6301 ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat tagcattatg
     6361 tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga ttgctacatc
     6421 tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga tcactaggtc
     6481 agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt ccaatggagt
     6541 gcctcccaag ataaattttt ataagtataa cataaattcg gcttttgcca atattttttt
     6601 gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt gtggagggga
     6661 gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt gccaaacatg
     6721 cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt cgctatggaa
     6781 aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg atctcaagat
     6841 ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta ggtgcattta
     6901 cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga gattaccttc
     6961 aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc catcgttgct
     7021 ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga ttataccttt
     7081 tcagcgggac agtcggatca ggcaagactt ctctaatgta tcggttggct ttggaagaaa
     7141 aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt tttctgcagc
     7201 tacagatcaa tccgaaaata gcacagactt atgatacact tttgaaactc gcattacggc
     7261 atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca catgctgcgg
     7321 taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc aatcttgaag
     7381 gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct gaatgtttag
     7441 ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct gttttatgga
     7501 gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag
     7561 ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag ccagcaaaac
     7621 cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca agaatccctt
     7681 tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat ccaacatcag
     7741 ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg agcagagcaa
     7801 ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt gttggaaatt
     7861 gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat
     7921 ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt gttcgttttg
     7981 cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca gctgatacaa
     8041 gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc tttactagta
     8101 aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag cagacaatta
     8161 ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga gattggaaag
     8221 ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga aacgaaacaa
     8281 gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc aggtatgtca
     8341 ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa aattgtttta
     8401 cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga atggacaaga
     8461 aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat catttttttg
     8521 ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt ttacggaagc
     8581 atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt tttacattga
     8641 tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc gtccctaatt
     8701 tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc cagttggtag
     8761 acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact gtggaggaat
     8821 tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt
     8881 aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt attacttaca
     8941 acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt ggcagtctat
     9001 caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat
     9061 cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt tcatgttccg
     9121 aatccagata aaataaattg ggaagtatta gatattcctg atgagattac cttgaaacgc
     9181 catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa agcttatcaa
     9241 ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg gagtggtcgc
     9301 tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta cagcgcttat
     9361 gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc ttcaggcaga
     9421 aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata tcaagacgca
     9481 gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg atatccaaat
     9541 tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag
     9601 tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt ggactgatga
     9661 tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc caagtaagtg
     9721 aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa ttagaagaag
     9781 aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac acgatcgagg
     9841 ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga acttccggcg
     9901 gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa gaacacacaa
     9961 tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg acaaaaaacg
    10021 atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt atttctttta
    10081 tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga tcttaatata
    10141 cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc ggaacagtct
    10201 acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt aacatatcag
    10261 tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt tcggttcaca
    10321 gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg tgaaaacgcc
    10381 ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca caaataaaaa
    10441 acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg tgaaaaaatt
    10501 gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga acaagggttt
    10561 aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc ctttttagaa
    10621 gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt ggtggatgga
    10681 gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt gaagaaactt
    10741 tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa gggcagccag
    10801 acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt tttatttgtt
    10861 tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct ggatattgcg
    10921 gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc ggggtaccaa
    10981 acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc ttctacaagt
    11041 gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga attattaata
    11101 gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa
    11161 gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca tttattgcta
    11221 gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat gccgagtgga
    11281 tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt
    11341 caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca aaaaagtatt
    11401 ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt gcttgtgaaa
    11461 cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt taaaaactgg
    11521 aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa aacaattgca
    11581 atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga agaaaccgta
    11641 gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac catcaagtac
    11701 ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt agcagtaaaa
    11761 atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga aatcaccgga
    11821 gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt gatcgatgag
    11881 aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa tcctgcaaac
    11941 ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt ggctgtattc
    12001 gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt accggtgtct
    12061 tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca tcgttatgtg
    12121 tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat catcacttgc
    12181 cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat ggatacttct
    12241 atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga tatcgatcca
    12301 gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat gatcaatgtt
    12361 ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat gcgtgatctt
    12421 gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt ggaccgcatc
    12481 cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat tgtctttact
    12541 gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg
    12601 tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt agatatctct
    12661 acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact aatgattgcc
    12721 cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa agatgaaacg
    12781 catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa aatggtaatt
    12841 taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct tggaaaagtg
    12901 gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc caatatttta
    12961 ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat ttttagatca
    13021 gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac cgatccgaaa
    13081 cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct gaatcgtgaa
    13141 agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca atcagttggc
    13201 cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg aagtgttgct
    13261 gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct tgcagcattt
    13321 cttcaatttc ttctcgagca tagctgtcga ggtgattggt tggttcatcc aaaatcagga
    13381 cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca ccaccggata
    13441 aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct aattgccgtc
    13501 gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat
    13561 cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct aatttgatgg
    13621 tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat tttcctgcac
    13681 cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg ctgtctttga
    13741 atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact tctttgccag
    13801 attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc ggtttgacga
    13861 gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag gactcattgt
    13921 ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc tggacttctt
    13981 tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc aagactcgaa
    14041 aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc catgtttttg
    14101 tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat ttttttgtat
    14161 tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg gtcggttcat
    14221 cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact ttaactcgtt
    14281 cgcctccgct aagttctgtt aaccgtgctt cctgacagtg tggaatccct aagccttgca
    14341 agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag cgatgctgca
    14401 gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc atcaatgctt
    14461 cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt
    14521 ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc tttaagcctt
    14581 ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg
    14641 ttgatttgcc agttccgttg atatcgacta aggcaatttt ttcaccagct tcaatttgac
    14701 aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa attgcttgta
    14761 tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatccc tggcctgtgt
    14821 tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat tttacgtgaa
    14881 ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct
    14941 aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca tgcgcatcat
    15001 agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt
    15061 caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag agagcttttt
    15121 ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc taaaccagcg
    15181 ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt ccttttgcca
    15241 tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc aacatcgcgt
    15301 tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat
    15361 ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg
    15421 gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga ttcatcggaa
    15481 acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa accacaccag
    15541 ggcgactaat cgccctggtg ttcacttttt ttattttact tgaactcatt gactgttttc
    15601 cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg tttttatcac
    15661 ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt taagctttgg
    15721 tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc acatcgcaag
    15781 ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct acagcattca
    15841 atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt tctctggcaa
    15901 taatttgttt tggtgagcca tattctacga cagaggttac attattcaag ccagctttct
    15961 tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct tgttctgcaa
    16021 gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca tgtgctaata
    16081 gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct aattcagctt
    16141 catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata agcattcact
    16201 cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa aagagaatta
    16261 tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg gcagaaacaa
    16321 taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa gctgttaaaa
    16381 tacccattac gcctaaaaca gcatatgcag caccgtaaaa ttgtaggaat ccttgatttt
    16441 tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa taatagctaa
    16501 cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac aaatcctcca
    16561 aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat tctttaaaag
    16621 atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg aattcaagca
    16681 ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta ccagcagtga
    16741 cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa gaggacaatt
    16801 cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga gcaatgagag
    16861 agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt tttccactct
    16921 tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc ttttggctga
    16981 taatactgag cattcttcag tttatctggt aaatattgct gattgaccca agcctgatca
    17041 aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc tttgtaatgg
    17101 ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc agcaagtgct
    17161 gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc gacgacgacg
    17221 tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa tacagcgttc
    17281 actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc gataaccatc
    17341 agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag ataatgaagt
    17401 gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac gtcataatga
    17461 gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc aatgatcgac
    17521 agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc cagttccagc
    17581 ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag aagagcatct
    17641 tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg
    17701 actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg tgtacggctg
    17761 cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat caagatgatt
    17821 cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa tcggtgtact
    17881 tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac aacttgtaga
    17941 tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt agagccggcg
    18001 atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat catggaagag
    18061 agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag atgctgctga
    18121 ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg ttgcatagga
    18181 ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag atgcctagaa
    18241 agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt gtatgataaa
    18301 gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga ataaaacgac
    18361 gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac cagaaggaag
    18421 attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg aggccgccca
    18481 tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat
    18541 ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc cagaagggat
    18601 tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc aaatgagtaa
    18661 aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat tagtggaagc
    18721 attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt tacgcttagt
    18781 ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg gtatcccaaa
    18841 caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc cttatatctc
    18901 tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga
    18961 aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac tatgatgtag
    19021 ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa
    19081 ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg
    19141 aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt
    19201 atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta gctgattctt
    19261 tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt gattttttgg
    19321 atgaagacga tgacgaagct gtattcgagc ttcactggga taatcaacaa ctagcagaat
    19381 tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca agttattaag
    19441 gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag
    19501 cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa gattctttag
    19561 atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat gattttacat
    19621 cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc ttaccggaaa
    19681 tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta gctattggaa
    19741 aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc tggaagaaat
    19801 actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg gaaaactacc
    19861 agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca tttggcacag
    19921 gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg cgtggtggtg
    19981 aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca aaatatcttg
    20041 gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa
    20101 atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat cttttgacag
    20161 gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc atcttattga
    20221 tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt tcaggaatca
    20281 ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt atcgtggatc
    20341 aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag gtggaataga
    20401 tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt ttagaagggg
    20461 aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa gtttttttgg
    20521 cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa gaggctgttt
    20581 atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg attaccatag
    20641 cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc actgaattag
    20701 gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa
    20761 aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg gaacaatccc
    20821 atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta tttgagcggc
    20881 tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat
    20941 caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta gctgtctttg
    21001 gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac aaagggatac
    21061 tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat ttattaagtg
    21121 ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct tcattaataa
    21181 aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata gaaacaatta
    21241 tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa catttccgta
    21301 aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt gtcccgattc
    21361 ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga aattttaacg
    21421 ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt
    21481 gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa
    21541 ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc
    21601 catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa
    21661 gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta
    21721 gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg
    21781 ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac
    21841 atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta
    21901 gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa
    21961 gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag
    22021 acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagcg
    22081 acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc
    22141 tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt
    22201 cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa
    22261 tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac
    22321 caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga
    22381 acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa
    22441 ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag
    22501 atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga
    22561 gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac tgagttacca
    22621 aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg taacaaaacc
    22681 actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggagat
    22741 atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta
    22801 gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa
    22861 gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg
    22921 cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa
    22981 acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc
    23041 aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca
    23101 gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt
    23161 ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg
    23221 attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt
    23281 cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc
    23341 gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc
    23401 tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat
    23461 ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt
    23521 ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat
    23581 gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg
    23641 ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct
    23701 acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa
    23761 aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga
    23821 agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa
    23881 aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga
    23941 atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac aattcggagc
    24001 agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa
    24061 caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttagac
    24121 tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt
    24181 cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt gctgaagatg
    24241 aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta tcaaaaagga
    24301 tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt
    24361 ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt ttatgatcca
    24421 aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact ttttggtcaa
    24481 ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat ttttatggat
    24541 ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa tctgacacaa
    24601 gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat ggttttcttt
    24661 acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa
    24721 aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc gattgatcac
    24781 ataaaaggtg tcgagaatcg caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa
    24841 gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc
    24901 acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt attgaacatc
    24961 atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc acttttcagt
    25021 agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga tccgtttatt
    25081 caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa
    25141 gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga tttatatgga
    25201 acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa acggtatgta
    25261 gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca aggcggggga
    25321 aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag tctacgtgtg
    25381 ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga ttggacgatt
    25441 ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa gaattagatt
    25501 agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt ttttcatatt
    25561 actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca atggtttgat
    25621 aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact tgctctggac
    25681 gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa gctaaaacaa
    25741 gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg acagcggtta
    25801 aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga tctccttcac
    25861 caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa gcaagaggaa
    25921 aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct ttttcagcct
    25981 gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct gcattcgctt
    26041 ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt cctgcttctt
    26101 ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc ggaaagtgga
    26161 tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc accgattgtt
    26221 tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga gcagcttttg
    26281 tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg ccaatcagtt
    26341 cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc ccgctattca
    26401 aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa agattatgtc
    26461 ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa tgtccgattc
    26521 ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat accaaaaagc
    26581 tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt gagactggtt
    26641 tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt
    26701 tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct aaagacactc
    26761 tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga
    26821 aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt catgatgaac
    26881 aagggtgatt tttttattta catagctaag aaagggagag aaaactatga gtttccaaag
    26941 accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga
    27001 aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac cgatttttga
    27061 acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt caaaagagat
    27121 gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag gtacagcacc
    27181 aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga aaccgttcaa
    27241 aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac ggttacgtca
    27301 attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg atgtggagag
    27361 catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc ggctagtcat
    27421 caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga tcgactatct
    27481 tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt
    27541 acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag ctccatctat
    27601 cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt ccatgctgga
    27661 tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag attattacac
    27721 acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag caacgatttg
    27781 cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa cacctggctt
    27841 cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg aagtagttat
    27901 ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa caaatatcga
    27961 agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc gcgacttcat
    28021 gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca aattagtatt
    28081 ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa tggcaagccg
    28141 aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag tctatgatga
    28201 aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat ctattgcgga
    28261 ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt acaaaaaaga
    28321 cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc
    28381 gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg tcgcagtgaa
    28441 tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt aaatcctaaa
    28501 atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa
    28561 accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg tttgaaatat
    28621 cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca
    28681 acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga aacaccttac
    28741 ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg tgtccatgct
    28801 ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct gatgaatgct
    28861 ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt acgtggagac
    28921 cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc agaagaaatc
    28981 caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc
    29041 acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa
    29101 ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa agaagtagaa
    29161 ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa
    29221 ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata tgtaggacaa
    29281 ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa aggtccaatt
    29341 gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc aaaaccaggt
    29401 gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc
    29461 cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa tttcttatgg
    29521 gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt ctctgctcac
    29581 catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga ccctgcaaaa
    29641 gtctatgcag aagcttatga tatcgtcttg aacggttatg aattaggcgg aggttctatc
    29701 cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa
    29761 caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt ccctccacat
    29821 ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga agataatatt
    29881 cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag tgatgcacca
    29941 agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa tatagaagaa
    30001 tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa ccttgattta
    30061 attgaaaaac actttataat gaagccataa catataaagc ggggcttttc catgaaaaaa
    30121 acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc tatcgtttat
    30181 gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat ttattcaagt
    30241 ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat aggattcgaa
    30301 gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt ggcgtggtta
    30361 aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac ctccctttgt
    30421 atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc gttgtttggg
    30481 ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc atcgggcggt
    30541 ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg
    30601 atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg gcaatatatg
    30661 ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa
    30721 caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc
    30781 caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc ttataagcat
    30841 gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct acttagagaa
    30901 gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt ccagatactt
    30961 ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg aaatactgct
    31021 gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca gcaaagaaaa
    31081 actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat ttttttaaat
    31141 ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc tattttcact
    31201 taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa
    31261 acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc
    31321 tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg
    31381 cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg
    31441 ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta
    31501 tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg
    31561 gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg
    31621 gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca
    31681 tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt
    31741 gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt
    31801 cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta
    31861 ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg
    31921 gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc
    31981 tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa
    32041 tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt
    32101 gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat
    32161 tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc
    32221 aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat
    32281 tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta
    32341 tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa
    32401 accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc
    32461 accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa
    32521 aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc
    32581 gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact
    32641 aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc
    32701 aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga
    32761 aaaattaaag gaacaagatt tgttgaaaga cactgttctg attggactag gaacgaatgg
    32821 ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata
    32881 ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga
    32941 acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga
    33001 ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta
    33061 cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac
    33121 aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa
    33181 gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga
    33241 gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc
    33301 ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct
    33361 tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga
    33421 aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata
    33481 atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt
    33541 aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa
    33601 ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg
    33661 caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca
    33721 tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg
    33781 tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg
    33841 gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga
    33901 cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa
    33961 gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag
    34021 ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa
    34081 caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa
    34141 aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc
    34201 gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata
    34261 ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat
    34321 gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga
    34381 gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa
    34441 gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg
    34501 gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca
    34561 gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga
    34621 ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg
    34681 tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga
    34741 atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg
    34801 ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag
    34861 aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa
    34921 ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag
    34981 tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa
    35041 aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa aatagaagac
    35101 aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt tgaggtgatt
    35161 gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa ggttatgact
    35221 tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa gaagaaaaag
    35281 tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct gttgatgccg
    35341 tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg
    35401 ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca tggttcagtt
    35461 attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc gagtttaaaa
    35521 acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta tattttgaag
    35581 atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa atgcaggagc
    35641 tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg ggaagtgtaa
    35701 aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct ggtgaactac
    35761 ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct ttgagacagg
    35821 ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc aaccaaaaaa
    35881 aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg gagatcgaac
    35941 ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat cctgcctttt
    36001 atcagtccgt caatcgggag aattacatct atatctatct cactgataga acgatcatcc
    36061 cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt gaacgggcaa
    36121 acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa
    36181 aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca aaccaagaaa
    36241 aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga atgaaaccga
    36301 aaaaattaca acttaaaaat tttggtcctt ttatcaatga gaccgtagat ttttcacgtt
    36361 tgacagaagc accgcttttt ttgatcagcg ggaagacagg agctggcaaa acgacgattt
    36421 ttgatgggat caccttcgca ctttttggtg aaacttccgg aagacttcgc tctggaaaag
    36481 agatgcgttc tttgtttgct acaccagacg aagaaacaag cgtgactttt tcttttgaac
    36541 atcagcagat gagctatgaa atcgaacgaa aaccagaaca agttttagca aaacgaaaag
    36601 gaaacggaac aaaaaaacag gcagccaaag taacgttgac catttttgat gggaaaggaa
    36661 aagaaataaa acaaatcaca aaacgtacag aagcagatca gttgatcaga gaactgatga
    36721 atctcgatgc gaaacagttt tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt
    36781 ttttggtttc atcaagcagt gataaagaga ctgttttacg tcatttattc ggtactcaat
    36841 ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg
    36901 atcatttaga gcaagagctg catcttctgc aaaaaaggtt tgttttagtc aaagaagagg
    36961 aaccagcagc agggtttgaa gccgttttag cccaatggac aaaccatcag gcaatactcg
    37021 aaaaacaaat cacggaagaa aaagaagctc taacggaact tcaggaaaaa caaaagcaac
    37081 tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat
    37141 taataaacaa gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa aagaaacgat
    37201 ggattgggta ttatgaattt acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa
    37261 taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga
    37321 tgactacgca gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa
    37381 caaaaatcaa agaagctgct caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg
    37441 aatccttacg tagtaaaaaa caagacgcag aacaattaac aatttcattg gataaagaag
    37501 aattgaatgc cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga actcgaattc
    37561 ttcaagaaaa tttactggat caggaaaaac tgcaagaaga tcgtcttgct tttggacagt
    37621 taaaatatct agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt
    37681 cgctcgcaca gataagagaa gaactcaaaa aagcaactga agaaataaca aaaaatagtc
    37741 agctattgaa agaacagaca gaacaagcag aacagctgaa aagccaatgg gcaaagcagc
    37801 aaattgctcg tcttcagctt tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa
    37861 cggaacaccc catacaacaa ttaaaacacg aacagccaaa taaggaagat atcttaaaag
    37921 tagaacaaca actggcagaa gctgagtctg ctgtagaaag aactcagcaa atcattgcca
    37981 aaagtgaagc acatgaaaat aatcttcaac aacaattatc agatttagaa caaaaaatca
    38041 tggcaacgaa tatacaattg gctgaacaag aagaacactt gcaacaggct ttgaaaaagg
    38101 catttcctga gataacagaa caaaaaacaa aaacaactct tgtagtaatc gaacaatctt
    38161 tagatagacg aaaaagcaag atggccaatg aaagaataga gctggaagaa ataagacaac
    38221 aagcagaaaa gctcgaacaa caattagctg tctacaaaca gcagcagaca gaaaaaaata
    38281 atcagctcca gcaactgcaa ggagaaatca aaagtttaga acaacaattg caagatacgg
    38341 atagtaactg tttagaagag gagatacgac gacttgaaaa acagttagaa gaagactcga
    38401 atcaactagc tgttgagcag aaagcaggag tgcaattgtt gcaagatctt catacgcttc
    38461 ggacacagct tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg
    38521 aacaagaaag aatagctaaa aagttagaaa gccagtcgta tttttcaaca attgaagaaa
    38581 tctgtgcatt tgcacaacag caagcacaat ataaagaaaa ccaacgtaca gttacttctt
    38641 atcatgaaga gcaattagtg acagctgacc ggctgcgtca attagaaaat atccagctaa
    38701 ctgagacatt cccagataca gaagagatca gccaacagtt agaagaaata agacgtatga
    38761 tcgaaataaa gcaagaaaag atatacggtt tacaagaaca aaaaattagt aatcaaaaaa
    38821 tctacgatga atgtctttcg atttatcaca caagccagac gaaattagat gagcttacgc
    38881 aattacagca gctttcgcaa acattcaacg gagaaaatcc taagaagact agtttggaac
    38941 ggtatgtgct tcaggtttat cttcaagaag tgttacaagt agcaaatgat catctccaac
    39001 gtttaacgaa aaatcgttat caatttgagc tagcggatac cattggcagt tatcgaggga
    39061 aaaccggatt agaaatcaat atctatgatg atcaggcagg tatgtctcga agtgcgcata
    39121 ccctgtctgg cggtgagagt tttattgcag cactttcttt agcattagct cttgccgatg
    39181 tgatccagac acaagctggt ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg
    39241 ggtcattaga tgaagaagct ttggaaatgg caatggaagc tttagaaacg atcgaaagtg
    39301 aaggaagaat gatcggcatc attagtcatg taagagaatt gaaagaacga gtattacaac
    39361 aaataagaat tgatacagaa ggtagcggac aaagcaaaat acgctatcaa cttggataaa
    39421 aagggagatt ggtaactatg aaatggagta ttccagaaaa agtcattgca cgtggacgtc
    39481 aatacttgca agaaggccga gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc
    39541 atgcagaagt cttgggcagt gaattgtata tggttgattt ggatggtaca gcaaaagaga
    39601 tagattactg ccaatgtccc tattgggagg aacataaata ttgtaagcat acagttgctg
    39661 tggaacttta tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc
    39721 tagaaccggt aaaagcaaca agtgagggag aaatcattgc aaaaggtttt tctcgtctaa
    39781 aaacacaaaa caatcctcaa gcggtgcaac ctttgattat tgaataccaa gtagaaacga
    39841 ttgagactaa tcagtatcac ccagagcttt ctattttggc actttacttg cgtatcggtt
    39901 cattggcatc accgaaaaaa acatatatcg taaaaaacat ctatgatttt ttacaagctt
    39961 acacagaaaa agaaacttac actgttaata aacagtacca gtttcgtcta gataaacggg
    40021 cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc aggaagtgct cagacgcatc
    40081 aattattagg tacgaacggc ttacaagtca aaggaaaact agataaaaga tatctgcttc
    40141 tcccagtaga acaagcccgc ttcctgttag agcagatgaa tcagactaca cgtttgcatc
    40201 tgcagataaa tgaacaaaac gatcaaggaa tacaattttc tgatcaaaac aaacctttat
    40261 cattcaaagt ggaaaaattt ggagaagatt atttgctgca agcattgaat gattttgatt
    40321 ttttctttat gcattatcag tggggagtta tccaagggac attttattct ctgacgaaat
    40381 atcaacaaac ggtttatcaa acttggaaac aattgattcg ccgattggag aaaccagaag
    40441 tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct ctgctgtcag
    40501 agatcggcga cgtagctatt gcccctgcag tagaagagga agtagcagat atccccgtcg
    40561 aatttatttt ctttttccga aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct
    40621 atggtgaagt catctattcg actgacccga agcatgaggt ctcgacagaa caggcttttc
    40681 aggtgttacg tgacacagta gaagagaaac gagtacttga tctattcaga atgtatcgct
    40741 accagaaaaa tgaaactggt tatgaacgtc cattgccaag cggggaagaa ctatatgcct
    40801 ttttccgcac agaactatct gtcttccgtc agtttggtga agtcagactt ggaagaaaat
    40861 tgcgagagtt gtatttagat gcgcataaac atcaaccgaa aatcgaagta accgagaccg
    40921 gttcatggct cgatattcga ttcgatgtga cagggataga ggagcaagaa atcgatcatg
    40981 tcctgcaaag cttgctgaga aatgatgctt tttatacatt agaaaatggt caggttcttt
    41041 cttttgattc agaagaattt caacaaacaa gccaagtgtt gcaacaatta cgggaatcga
    41101 tacgtacaga agaaggaacg atccatgtac ctaaaaatca aggattgatc attcaaaatc
    41161 aattggaaaa aagcaatgct acttttagtg aatcgttcca aacgatggta caagatctca
    41221 ttcatccaga aagataccag gcacagcttc ccaaaggatt gaatgcaacg atgagagatt
    41281 atcaaaagca aggttttcga tggttgaaga tgttaggtca ttatcagttt ggcggtattt
    41341 tagccgatga aatgggactt gggaaaacac tacagacaat tgccttttta ctttcggaaa
    41401 aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta atttacaact
    41461 ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc aatgggaata
    41521 aaaaagaacg tgaagaatta ctggctaaag acacagatat ccgagtgact tcttatgcca
    41581 gcttaagaca ggatctagct aattatcagt cgcaaaaaat tgattatttg attcttgatg
    41641 aggcacaaat ggtcaaaaat agcagtacga aaactgcaca agcattgaga gaattagctg
    41701 tacctcagcg gtttgcactg agtgggacac caatcgagaa taatttagaa gaattgtggt
    41761 cattgtttgc aacgattatg ccaggatttt tcccgactaa aacaaaattc agggaacttg
    41821 ccgcagaaga aattgcgcaa atgattcgac cgtttatttt acggcgagat aaacaaactg
    41881 tgttgaaaga cttgccggaa aaaacagaga tgaatctata ttctgcactt acagaagaac
    41941 aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt
    42001 ctgaagcatt caagaaaaac cggatcggga tacttgctgg tctgacaaga ctgcgacaaa
    42061 tttgctgcga tcctcgtctg tttattgaag attaccaagg cggttccggc aagctcgaac
    42121 aagtaaaaga tctactggta gctgccaaag agaacaaacg aagagttttg ctgttttctc
    42181 aatttacagg gatgctgact atcttgcaag aagagttagc cgagctaggg atctcgacat
    42241 tttatttaag aggcagtacg aaaccacaag atcggttaag tatggtagac gcatttaatg
    42301 ctggcgaaaa agatgttttt ctgatttcct taaaggctgg gggaactgga ttgaatctca
    42361 cgggagcaga tacggtcatc ttgtatgacc tttggtggaa tcctgccatt gaagaacaag
    42421 ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct
    42481 ctgaaggaac gattgaggaa cgcatggatt cactccagca agaaaaacgc gaactgttcc
    42541 aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact gactgaagaa gacatccgca
    42601 tgattttaag tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt cagtaatggg
    42661 taagaaagcc tttctaaaga agcgattgtt tatttgattt aaaaaatagc aaggaggctg
    42721 ggattccatt ttatcccagc ttccttgcta tcattttttt atttctattt ttaaaggtta
    42781 tacaaagatt gtcttcattg tctctaacag ctgatcaaaa gaagaaatct cgatatcgca
    42841 aattggagtc tgttcaattt tgcgttctcg atggttgaac caaagcgctt gccagttagc
    42901 actttttgca cctagaacat cattgtcgta attatcgcca acgtataagg tctcttctgg
    42961 aagcatatga aattctttct cagccagctg aaaaatctct ttttctggtt tttggaaacc
    43021 tgttgcttga gaaatgagca tattgcggga aggaatccaa ttgttcaatt gaagttgctt
    43081 cagttttttt gtctgatggt cagtcggccc atttgtgata attcctaatg ggacattctt
    43141 ttccttcaag aaatcgaggg tttttttgac ctcctcatgc aagcagatat tatcaagttc
    43201 ttcctcgtaa attttttgaa ataagaggcc atcttcttcc gtaatatgag ggtagtctaa
    43261 atcttttagt gattggctga tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc
    43321 cattactttt gggaagtttt catcactatg atggcggaac cggatatata gtggatgcat
    43381 atctgcatct gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg gctgctgctg
    43441 atcatacatc gtatcatcaa catcaaacac aactgattta atcaaaattt tacacgcctt
    43501 tcggttttca ttgatttttt cacaaatcct tgactattgt aacagaaaaa attgtctaaa
    43561 aaagctgata aataaaggtt tttcttacta tatttctatt tccttgacag aataaggttt
    43621 ttatatttca gtttttttca ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa
    43681 ttaatcatta tggtagaagt cgattgattt attttaatgc aatctctgtg gaattttcga
    43741 aagatttat
//
LOCUS       DAZKAS010000008        43399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000008 DAZKAS010000000
VERSION     DAZKAS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.008"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..395
                     /locus_tag="ACG8H7_000332"
     CDS             21..395
                     /locus_tag="ACG8H7_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFP8982468.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            534..779
                     /locus_tag="ACG8H7_000333"
     CDS             534..779
                     /locus_tag="ACG8H7_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP8982469.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            951..2015
                     /locus_tag="ACG8H7_000334"
     CDS             951..2015
                     /locus_tag="ACG8H7_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFP8982470.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            2261..2686
                     /locus_tag="ACG8H7_000335"
     CDS             2261..2686
                     /locus_tag="ACG8H7_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP8982471.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            2679..3128
                     /gene="nusB"
                     /locus_tag="ACG8H7_000336"
     CDS             2679..3128
                     /gene="nusB"
                     /locus_tag="ACG8H7_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP8982472.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            3225..4079
                     /locus_tag="ACG8H7_000337"
     CDS             3225..4079
                     /locus_tag="ACG8H7_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFP8982473.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            4080..5417
                     /gene="xseA"
                     /locus_tag="ACG8H7_000338"
     CDS             4080..5417
                     /gene="xseA"
                     /locus_tag="ACG8H7_000338"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP8982474.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            5422..5646
                     /locus_tag="ACG8H7_000339"
     CDS             5422..5646
                     /locus_tag="ACG8H7_000339"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP8982475.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            5648..6538
                     /locus_tag="ACG8H7_000340"
     CDS             5648..6538
                     /locus_tag="ACG8H7_000340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP8982476.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            6547..7365
                     /locus_tag="ACG8H7_000341"
     CDS             6547..7365
                     /locus_tag="ACG8H7_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFP8982477.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            7472..7921
                     /locus_tag="ACG8H7_000342"
     CDS             7472..7921
                     /locus_tag="ACG8H7_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP8982478.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7939..9621
                     /gene="recN"
                     /locus_tag="ACG8H7_000343"
     CDS             7939..9621
                     /gene="recN"
                     /locus_tag="ACG8H7_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP8982479.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(9676..9792)
                     /locus_tag="ACG8H7_000344"
     CDS             complement(9676..9792)
                     /locus_tag="ACG8H7_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFP8982480.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9865..10821)
                     /locus_tag="ACG8H7_000345"
     CDS             complement(9865..10821)
                     /locus_tag="ACG8H7_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP8982481.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10992..11360
                     /locus_tag="ACG8H7_000346"
     CDS             10992..11360
                     /locus_tag="ACG8H7_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFP8982482.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            11530..11961
                     /gene="mraZ"
                     /locus_tag="ACG8H7_000347"
     CDS             11530..11961
                     /gene="mraZ"
                     /locus_tag="ACG8H7_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP8982483.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11982..12941
                     /gene="rsmH"
                     /locus_tag="ACG8H7_000348"
     CDS             11982..12941
                     /gene="rsmH"
                     /locus_tag="ACG8H7_000348"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP8982484.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12946..13347
                     /gene="ftsL"
                     /locus_tag="ACG8H7_000349"
     CDS             12946..13347
                     /gene="ftsL"
                     /locus_tag="ACG8H7_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP8982485.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            13348..15540
                     /locus_tag="ACG8H7_000350"
     CDS             13348..15540
                     /locus_tag="ACG8H7_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP8982486.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            15566..16528
                     /gene="mraY"
                     /locus_tag="ACG8H7_000351"
     CDS             15566..16528
                     /gene="mraY"
                     /locus_tag="ACG8H7_000351"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP8982487.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            16542..17903
                     /gene="murD"
                     /locus_tag="ACG8H7_000352"
     CDS             16542..17903
                     /gene="murD"
                     /locus_tag="ACG8H7_000352"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP8982488.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17929..19017
                     /gene="murG"
                     /locus_tag="ACG8H7_000353"
     CDS             17929..19017
                     /gene="murG"
                     /locus_tag="ACG8H7_000353"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP8982489.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            19032..20252
                     /locus_tag="ACG8H7_000354"
     CDS             19032..20252
                     /locus_tag="ACG8H7_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFP8982490.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            20408..21733
                     /gene="ftsA"
                     /locus_tag="ACG8H7_000355"
     CDS             20408..21733
                     /gene="ftsA"
                     /locus_tag="ACG8H7_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP8982491.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            21756..22997
                     /gene="ftsZ"
                     /locus_tag="ACG8H7_000356"
     CDS             21756..22997
                     /gene="ftsZ"
                     /locus_tag="ACG8H7_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP8982492.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            23014..23691
                     /locus_tag="ACG8H7_000357"
     CDS             23014..23691
                     /locus_tag="ACG8H7_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFP8982493.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            23705..24304
                     /locus_tag="ACG8H7_000358"
     CDS             23705..24304
                     /locus_tag="ACG8H7_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFP8982494.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            24385..24606
                     /locus_tag="ACG8H7_000359"
     CDS             24385..24606
                     /locus_tag="ACG8H7_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFP8982495.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            24655..25437
                     /locus_tag="ACG8H7_000360"
     CDS             24655..25437
                     /locus_tag="ACG8H7_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFP8982496.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            25458..26165
                     /locus_tag="ACG8H7_000361"
     CDS             25458..26165
                     /locus_tag="ACG8H7_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFP8982497.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            26427..29225
                     /gene="ileS"
                     /locus_tag="ACG8H7_000362"
     CDS             26427..29225
                     /gene="ileS"
                     /locus_tag="ACG8H7_000362"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP8982498.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(29274..30800)
                     /gene="zwf"
                     /locus_tag="ACG8H7_000363"
     CDS             complement(29274..30800)
                     /gene="zwf"
                     /locus_tag="ACG8H7_000363"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP8982499.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(30815..31462)
                     /locus_tag="ACG8H7_000364"
     CDS             complement(30815..31462)
                     /locus_tag="ACG8H7_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFP8982500.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(31646..31975)
                     /locus_tag="ACG8H7_000365"
     CDS             complement(31646..31975)
                     /locus_tag="ACG8H7_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFP8982501.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            32152..32880
                     /gene="sfsA"
                     /locus_tag="ACG8H7_000366"
     CDS             32152..32880
                     /gene="sfsA"
                     /locus_tag="ACG8H7_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP8982502.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32896..33909
                     /locus_tag="ACG8H7_000367"
     CDS             32896..33909
                     /locus_tag="ACG8H7_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8982503.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33909..35186
                     /locus_tag="ACG8H7_000368"
     CDS             33909..35186
                     /locus_tag="ACG8H7_000368"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP8982504.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            35249..37951
                     /locus_tag="ACG8H7_000369"
     CDS             35249..37951
                     /locus_tag="ACG8H7_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8982505.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            38103..38420
                     /locus_tag="ACG8H7_000370"
     CDS             38103..38420
                     /locus_tag="ACG8H7_000370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8982506.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            38450..38770
                     /locus_tag="ACG8H7_000371"
     CDS             38450..38770
                     /locus_tag="ACG8H7_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP8982507.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38878..40338
                     /locus_tag="ACG8H7_000372"
     CDS             38878..40338
                     /locus_tag="ACG8H7_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8982508.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            40406..40627
                     /locus_tag="ACG8H7_000373"
     CDS             40406..40627
                     /locus_tag="ACG8H7_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982509.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            40658..40840
                     /locus_tag="ACG8H7_000374"
     CDS             40658..40840
                     /locus_tag="ACG8H7_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFP8982510.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            40840..41253
                     /locus_tag="ACG8H7_000375"
     CDS             40840..41253
                     /locus_tag="ACG8H7_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFP8982511.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            41376..42557
                     /locus_tag="ACG8H7_000376"
     CDS             41376..42557
                     /locus_tag="ACG8H7_000376"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8982512.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(42628..42792)
                     /locus_tag="ACG8H7_000377"
     CDS             complement(42628..42792)
                     /locus_tag="ACG8H7_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982513.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42946..43019
                     /locus_tag="ACG8H7_000378"
     tRNA            42946..43019
                     /locus_tag="ACG8H7_000378"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42980..42982,aa:Arg,seq:cct)
     gene            complement(43088..>43399)
                     /locus_tag="ACG8H7_000379"
     CDS             complement(43088..>43399)
                     /locus_tag="ACG8H7_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8982514.1"
                     /translation="NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRH
                     THASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKND
                     N"
BASE COUNT        14501 a         7343 c         9328 g        12227 t
ORIGIN      
        1 caaatatctg gagggagaaa atggttcaat caatcaatac aaaagtagac ttggtaattg
       61 atgctacttc gtttacgggt ctgacagatt acgggcaaat catgataggt gataatggtt
      121 ttgagtttta ccatgcgcga gatagacgca aatatatcca aattccatgg gaagaagtgg
      181 attatgtact agcatcagtc atgctaaagg gaaaatggat tcctcgatat gccattcaaa
      241 cgaaaaaaaa tggtacatac acattttctt ccaaagaacc taaaaaagtg cttcgtgcga
      301 tcagagaata tgtagatgaa gatagaatgg tacaatcatt gagtttcttt gatgtagtga
      361 aacgtggtat tcgtagtatg tttaaacgca agtaaaaaat aaagaaaact tctaagagga
      421 ggttttcttt atttttttac tttgcagaag aaaatatgaa atagcaaaac caattatttg
      481 tcccctttaa atcccatatg ctaaaattga ttagcaaaga aggaggttat attatgagtt
      541 ggttatggac attgattgtt ggagccgtga tcggagcaat tgcaggtgcg atcaccagta
      601 aaggaaattc gatgggctgt gtaacgaata tcattgctgg actagtcggt tcaagtattg
      661 gacaagcgat tttcggcgaa tggggaccaa aagctgcagg tatggcactt attccatcta
      721 ttttaggcgc agtgattttg gtagcagttg tctcattttt ctttggaaaa cgatcataag
      781 tttttacaaa atagtaaact aaaaggagtt gcagcgagac tgtgactcct ttttggcttt
      841 atttatttaa aataaaaaga agttttccgg aaatttttat ttaactgtcg tgtacgaaga
      901 aaagtttgct ataatggaaa aaagcgtgca aagaaagagg gagtttcatt atgatgctac
      961 gtgtagaaaa attgcgcaaa aaaatgcaag aagaaaattt ggattcattt ttggttacca
     1021 gtccgtacaa tcttcgttac ttaacgaact ttacaggtac gacaggatta gctgttatta
     1081 ctttggaaaa agcatttttt attactgatt ttcgttatac cgagcaggcg gcagcgcagg
     1141 ctcaagggtt tgaaattatt aaaaatgtcg gacctatttt tgatgaggta gcaaacttag
     1201 tacgcaaaga agaactgcac tcattagctt ttgaggaaac aacagtctca tttttagaat
     1261 attcggtttt agaagaaatc atcgatgcgg aattagtacc tgtttcacag atgatcgaag
     1321 aactcagaga agtgaaagat gaagaagaaa tcgctattat cgaaaaagca tgtcatattg
     1381 cggatatggc atatgatcac atcctgaaaa tgatccaacc gggcatgact gagattgaag
     1441 tcgcaaatca actagacttt tatatgcgtt ctcttggcgc ttcaggtgtt tcttttgata
     1501 cgatcgttgc tagcggattg cgttcggcta tgccgcatgg tgtagcaagc aaaaaaatca
     1561 tcgaacaagg tgacttgatc acattggact ttggttgcta ttacgaaggg tacgtttccg
     1621 atatgacacg tacattcgct attggtgatc ctggggaaaa attaaaagaa atttatcaaa
     1681 tagtcttaga tgctcaatta gcagttattg atgctgcaaa accaggaatg actggtgtcc
     1741 aattagatgc agttgctaga gattatattt caaaacatgg ttacggggaa gcatttggac
     1801 attctacagg acatgggatc ggtttagaga tccacgaagg accaaacgtt tcttctcgtg
     1861 cagaaaagca atttgtacca ggaaatgtga tcactgatga accagggatc tacttaccag
     1921 gaatcggtgg agtacgtatt gaggatgact tgttgatcac agaagatgga aatcgtgtgt
     1981 tgactcgttc accaaaagaa ttgatcatct tatagtttca tcaatagaag aagtaagcgt
     2041 aaaaatcagt gttcacagac tttgttatag aaaatcactc gatttaatga tacactatag
     2101 aagaactttt aagctgtaag agataataaa gttgggtgtt ttctccaatt catagcagtt
     2161 tgcagctgat cggcggcagt tttcttacca cgtgatcact atgccgcatc atagagaatt
     2221 cggatgtatc tcgtgcttag gcaaagggag gaataaataa atggcagatg aaaaaaattt
     2281 gattttaaac gcaaaccaag atttaggaga gatcgtgatc gctcctgaag tcatcgaagt
     2341 aattatcggg attgcagctt ctaaagtaga aggcgtatac ggaatgcgcg gaacttttgc
     2401 cagcaatgtc actgaattat taggacgtgc agctcacgga aaaggtgttt acttagcaaa
     2461 cgaggaagat ggcttgaaag tagatcttta ctgttatcta gaatatggtg tttcagttcc
     2521 taaagtagca atggaaatgc aggaacgggt cagccagcaa gtccttttca tgacggatat
     2581 cgatttatct gaagtcaata tccatgtagt agctgttgtt cctgaaaaat tagaacaacc
     2641 aacaatcgaa gaactttttg aagacgaaga ggaagagaat gaataaagaa ttatcaagac
     2701 acgagattcg ggaaatggca ttgcaggctt tatttccatt agacttcaat gcagacctaa
     2761 caaaagaaga tgcgattttc aacgcaatcg aattagacca ccgcgacatg atcaacgaag
     2821 atgaatcaga gtttgttcct gtttatcttg atacgctagt tggcggtgtc tgtgcaaaaa
     2881 aagatgaatt ggataaagtg attgaaaaac atttaaaaaa caattggcac atcaatcgga
     2941 tctcaaaaat ggatttggtg atcttgcgta tcgcgatttt tgaaatgact tatgtgacag
     3001 atgtaccagc ggctgttgct ttaaatgaag caatcgagtt ggcaaaaaca tttagtgatg
     3061 accgttcacg taaattcgtc aacggtgttt tatccaatgt attgaaagaa ttagaagctg
     3121 gggcgtaagt cctagctttt ttctatgaat catcataaac ctgttataaa tcgggaaaat
     3181 tctatggtag aatgaaaaca caaaaaatat ctgggagtgg ttagatggca gaattgatta
     3241 acggaaaaga attagctgaa aaaatgcagg cagagattgc tgaaaaaatc caaaaattga
     3301 aagataatgg tatccatccc ggattagtcg ttttattggt aggggaaaat ccagccagcc
     3361 aaatctatgt aagaaataaa gaacgtgtag caaaagaaat cggaatccat tcattggtag
     3421 agcgttatcc tgagacgatt tctgaagacg aactacttgc agaaattgaa aaatacaatc
     3481 aagatcctag ttttcatggt atattggtcc aattaccatt gccaaagcat atagatgaag
     3541 aaaagatcct gttagctatc cgccctgaaa aagacgtaga cggttttcat ccgatgaatt
     3601 taggcagact ccttgcgggt aaaccggaca agattccttg tactccatac ggcatcatga
     3661 aaatgtttga agcttattct atcgatccgg cgggtaaacg agcagtagtt atcggacgaa
     3721 gcaatatcgt cggtaaaccg atggctcagc tactcttgat ggcagatgca actgttactg
     3781 ttgctcattc taaaaccgaa aatctggctg aactggcaca agaagcagat attttagtcg
     3841 tagcaatagg acgtggacat tttgtcacaa aagaattcgt aaaaccaggt gctgtagtga
     3901 tagatgtagg gatgaatcgt gatgctaacg gtaagctgat cggtgatgtg aaatttgatg
     3961 aagtggagcc tattgcaggc tggatcactc ctgtgccaaa gggtgtaggg ccaatgacta
     4021 tcacgatgct gatgtatcaa accgtaaaat ctgccgaagc ttttggagca ggtgaataga
     4081 tggcacaaga atatttaact gtcactacat taacgaagta tttgaaaaga aaatttgatg
     4141 ccgatcctta tttagaacgc gtatatttaa caggcgaaat atcgaatttc cgtttacgtg
     4201 ccaatgccca tcaatatttc agcttaaaag atgaccatgc aaaaatatct gctattatgt
     4261 tcaaaggagc attccaaaaa ttacgattcc agccaaaaga aggaatgaaa gtccttgtcg
     4321 ttgggcgtat ttcattatat gaagcgggag gttcttatca aatctatgta gagcacatgg
     4381 agcctgatgg aataggtgct ttatatcaag aattggaaga acgaaaagaa aagctgatga
     4441 aggaaggcct ttttcaaggg cctaaaaaaa tgctgccacg tttcccaaag cgaatcgcgg
     4501 tccttaccag tccaagcgga gcggttatcc gcgatatcat cacgacggtg aaaagaagat
     4561 atccaattgt ccaattggtt ttgttcccaa cagttgtaca aggaaaccaa gcagcggaca
     4621 atgttgtcag gaatatccag agagtagaag agatgggcaa tttcgataca atgatcatcg
     4681 gtcgtggggg tggttcgatt gaggatttat ggccgtttaa tgaagagcgg gttgctcggg
     4741 cgatttatca agctaatacg ccggtgattt catctgtagg acatgaaaca gataccacga
     4801 tcgctgattt agtagcagat gttcgggctg ccactccaac agcagctgca gaattagctg
     4861 ttcctgtttt gtcagaagag ttgatgcgca ttcaagaacg gcaagctaga ttgcagcagg
     4921 cgtttgtacg ccagatccag cgcaaacaag aacggttcga gcgtgctcga cgttcctatg
     4981 tatttcggca gcctgaaaga ttgtatgaag gtcagaccat caaattagac cagttgaaac
     5041 agcgattata tcaagctaca cagcaaattt atcatgcgaa agaaaaacaa acgatcgcat
     5101 taagccacca attggaacaa gtggctccgc tttatcgagt taaagcagct cgccaagaaa
     5161 ctgcttatct agaaaaacgc ttagaagaga aaatccaaca atatatgaaa caacagcagc
     5221 agcgttttgc tcaagctgtt caatctcttg atttgttgag tccattgaaa atcatgggaa
     5281 gaggctacag ctatacgacg ttaaatgatc aagtagtaaa aagtgtcacc gagattcatc
     5341 aaggggattt gctgacgatc cattatcaag atggaatcgt taaaggacaa gtacaagaga
     5401 tagacaaagg agaataaaaa tatggaaaat ccaacttttg aagagtcact gcaagaatta
     5461 gaaaaaattg tcatgcagtt agaacaagga gacgttccat tagaatcagc tctagattcg
     5521 tttaaacgag gaatggaatt aagcaaacat tgccaagata cattagctaa agcagaagag
     5581 accttgacga aaatgatgac agaatcaaat gaagaagttg attttgacgg aaacgaggaa
     5641 agctgagatg agattaactg acttttcagc tattcatcta cctgctgtag aaaaagaaat
     5701 cttgtctttt ttagatgagc atacaacgga tagaggactc tatgatgcga tgagttattc
     5761 agtgaaagca ggcgggaaaa gaatacgtcc cttattactt ttaacggccg ttgcttcctt
     5821 tgatgagcct attgatgttc ccgtctatca agtagcagct gctttagaaa tggtgcatac
     5881 gtattccttg atccacgatg atttgcctgc gatggacaat gatgatcttc gtagaggaaa
     5941 acctaccaat cataaagtat tcggcgaagc attagctata ttggccggag atggtttgct
     6001 tacaggagca ttccagctaa tcagtatggc acacttaggt aattctccta agcttctgct
     6061 gcttcagcaa ttagctgttt gtgcgggatc acaaggtatg gttgctgggc aagctgcaga
     6121 tatcgagggc gaatcgaaaa agctttcttt agaagaatta gcatttattc atgaaagaaa
     6181 gacagggcat ttgattcgtt atgccttgct ggctggtggc atattagcga agcaacctga
     6241 agaaatctta ttgctacttc aacgattagc cgaacattta ggtttagctt ttcagataag
     6301 agatgatcta ctcgatgtga ttggtacaac gaaaacatta gggaaaacag ctggtaaaga
     6361 tgaacgtatg gaaaaaaata cttacccgcg tttattaggc ctagaaaaga cacgtgaggc
     6421 gttggagatt gagctgttat ccgccaacaa gatcatagac aaattagaag aggacgtttt
     6481 ttcttttgat ggcgggctga tgcgccagat gatcaagcaa tttgatgtgg agaaatgagg
     6541 gaaagaatgg aaaaagaacg cgtagatgta ttagcggtca aacaaggatt atttgaaaca
     6601 agagaacaag ccaaacgttc agtgatggct ggtttgattt acaacgaaaa aaacgagcgg
     6661 tttgataaac cgggagaaaa aattccagtg agtagtgaat taagagtcaa aggtaaaaaa
     6721 cttccttatg tttctcgagg gggattaaaa ttagaaaaag cactcaaagc atttgatcta
     6781 tctgttgaag gaaaaacatt gttagatatt ggtgcatcca ctggcggatt tactgatgca
     6841 gctttgcaaa atggcgcaaa aatgagttat gctttagacg ttggctacaa tcagctagct
     6901 tggaaaataa gacaagatcc tagagtcgtc gtaatggaaa gagtgaattt tcgttatgca
     6961 aaacccgaag actttacctt aggaattcca gaagttgctg ttatcgatgt ttcttttatt
     7021 tcactaaaac tgatgctgcc tccattacat gctattttaa aagaagaggg agaagtgatt
     7081 gctttaatca aaccccaatt cgaagcaggc agagaagctg tagggaaaaa cggaattgtt
     7141 cgtgaccctc agacccatca aaaagtttta gaagacattt tgtcatttgc ggcacaaggt
     7201 ggatatgatg tgctggaatt aagttattct ccgatcacgg ggggagaagg aaatatcgag
     7261 ttcttggctc acttgaggaa agtacccgaa agcggaacaa tcaattcagc tattaatatg
     7321 gcagaagtgg tttcaaatgc acacgaacag tttgaccata aataaattgt aactgtgaaa
     7381 atcaagtgag aagtatgcgg attcttcttt attataccaa ggaaataagc tatcattgat
     7441 taagaagaat atgctaagga gcgaagacgt aatgagaaaa aaagatcggc atcgtttgat
     7501 tactcgattg ttgagtgagc aagatatccg aaagcaggaa gaatttgttg aaatcctgaa
     7561 aagcaaagga atatctgtta ctcaagctac gatttccaga gatataaagg aactgaaact
     7621 gatcaaggta ccggcttcag atggcggcta ccgttatagc ataccagctg aaacaaacga
     7681 agatgtaaat actaaattgg aaaaactatt aaaagacgct ttcgtggcag ttgaccaaat
     7741 ggaaaaattt gttattttga aaacattgcc gggaaatgct tcggcagcag caaatttgat
     7801 tgataaacgt tacaagaaag aattattttc gattatcaat gatgacgata atgttttgat
     7861 gattacacga actgaagacc aggcagtttt attgaagaaa gacttcctgc attaccttta
     7921 agttgaggag cgaatgtgat gctacaagaa atcagtatta ctaactttgc gattattccg
     7981 gaattgcggc tttcttttca cgaaggaatg accgcgctga ccggagagac tggtgcgggt
     8041 aaatccatta ttatcgatgc tttaggtctg ttagcaggag gaagaggttc aagcgattat
     8101 atacgtcaag gagcagaaaa atgtgtgctt gaagggttgt ttgaactacc aaaacaagaa
     8161 ggattctctg aactgatggt tgaattaggg atcgaaacag atgaggacaa tctgattgtt
     8221 cgacgggata tgtccttaac tggaaaaaat gtttgtcgag tgaatggtca tattatcaca
     8281 ttagctaatc tgagaagaat cggcagttat ttagtagata tccaaggaca aaacgaacat
     8341 caggaactgc tccagcctga atcacatctt gctttattgg atcgttttgg agatgtagct
     8401 ttccaacaga agaaaaagag ttatcagcaa gaatatatct cttatcgtga gctggaaaaa
     8461 agggtacgaa aagtgcagca aaatgaaaaa tcctatgttc agagaataga tatgctccat
     8521 ttccagcaag aagaaatagc tgctgctgat ttgcaagtcg gagaagaaga gaaattaaaa
     8581 gaagaaagag aaaaacttag caattatcaa aaaattgctg atggattagc tgcaggttac
     8641 ggagcactga cgaataatga acaaaacagt gtagatggtg ttggcttggc cgtttcagaa
     8701 ctgcaaggga tcgcccatct agatgtggag tatgaagcaa tttatgaaaa tatccaaagt
     8761 gcttactatt tgctgcaaga tgcaatcggc gatatgagtc gtcagattga tttattggag
     8821 ttagatgaga gtcgtttaga agaagttacg caacgtttag aattaatccg acagttgaaa
     8881 cgtaaatacg gcgaatccat tgagtcaata ttggcttact atgacgaaat caccgaagag
     8941 ctagcttctt ctgatttttc agaaggtcaa ttggacaaaa tgaaagagga actagagcaa
     9001 aaagagcttc tacttcagca acaagccgca gatcttcatg aagcgcgtaa aaaaatcgca
     9061 aaagaattgg aaaaatccat tctgcatgaa ttgaaaagtc tgtatatgga aaacactgag
     9121 tttgaggtac gtttcttaaa agaagaaaat agacaattga atcatgatgg atttgaccaa
     9181 atcgaatttt acattacgac caatccagga gagccactga agcctttggt gaaagtcgct
     9241 tctggtggcg aactgtctcg gatgttgctt gctttgaaaa ctattttttc ttctgagcaa
     9301 ggtgtgacaa gtattatttt tgatgaagtg gatacaggtg tgagcggaag agttgcacag
     9361 gccatcgcag ataaaatttc tcaaatatcg aaatactctc aagttttgtg catcactcat
     9421 ctgcctcaag ttgctgcagt cgccgactat caatattata ttgtgaaagc agtgattgat
     9481 ggacgtacgc aaacttctgt ttcagagttg aaaacaaaag aaagagaaga agaaattgca
     9541 cgtatgctgg ctggaagtga gattacaaaa ctcacactcg aacatgcaaa agaactattg
     9601 aaactcgcaa aaaggacctg agccacctcg cagtggttca ggtccttttc agcagctgat
     9661 agctatattt tatttttaca tgacactcat tacagcagtc aatacaacag atccaatcgt
     9721 taaaatcagc attgtccaaa tgacaaattt tgttacttta gcaaaagtac ttgtttgttt
     9781 tttttcacgc attgttttca acctcgtcat taattagttg attcaagtgt acaatctttt
     9841 ttccatttcc acaagagggc ttttttataa aaacttccct ttttttaaat aataggtagt
     9901 tacgatacaa ataaataatg ttaatagaac tagccatata aaggcatcat ccctttcagc
     9961 aataggtaac ttaatgttca ttccatagat actgccgacg attgtcggta tagttaaaat
    10021 aatcgttaaa gaagttaaaa ttttcataat attattcaga ttattcgata cgattgctga
    10081 aaaagtctga ttcatttgtt ctactagttt taattgaatg cgagaagtag tcattgcctg
    10141 ctctgtttcc accaatacat cacgtaaatg atttttcaaa gcatgatttt cttggaaaag
    10201 tttattttgc gctagttctt tcaatgcatc taaattagca gaagtcgctt ctctgaaata
    10261 aacgaggctt ttttggatat ccatgatttg ataaagttgt ttgttttcag tggatacttg
    10321 aagtttggct tccaaagtat cgcattgaga ttgaagcgta gctaaataac tgttgtaaga
    10381 gagtgtgacc tgccaaatga attgaaagaa gatattgaga ttaggcgaaa tatcactttt
    10441 tggaaaggtc tggctaaaga ctttctttaa aaacagtggt tcatgattcg taattgtgat
    10501 aagcttcttt ttgggcgtca aaataataga gagcgggtat gtatccactt gaaaataacc
    10561 gctaggactt gttttagcaa aaggatattg taacaataat aacgctggct ttgtcaactt
    10621 tgattgattg aattcttctg tacgggaatt ttctgcatcg tccaatatgg aagtgatgta
    10681 atctgacgga atttcaaatt gatgtgtcag ctgatgaatc tcttcttgat taggacgttc
    10741 tacacaatac cagatcgttt ggtcagtttc ctttccggta ttacgcagtt tctcattatc
    10801 taatgtaaaa tatttgatca tgattttcct cctgttgcct gtttctttta ttataacgga
    10861 tagggaattg gaatacatgg aatgtgtttg taagatatag aatataactg cataaattta
    10921 aataaaatga aacagttgtg taaagtatat acagtatact tgaagcaacg ataaaaaagg
    10981 agtttacgtt catgggcaca ttcacaccac ttatcttgtt ttggtatatt tacccggtaa
    11041 tcgttttgtt tggttgccaa tttcttgtaa aattattttc gttaaatcgt cgttttaaaa
    11101 taaaagcccc tgatttggcg attccttttt tgtggggcgg tatgcatgca ttgtctagaa
    11161 atacagggac aatttctatc ttaccgtttt tctttatttc tgttttgcta atgggaattt
    11221 gtattgctgt tttccaagca tattattatg aagaaattat ttatccgcgt tatttcaaga
    11281 tgacttggcg tttggttttt ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga
    11341 acatactttc ttacctatga aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt
    11401 ttgcagcttg atttttaaaa aaatgtttat gaataaaaaa agttgttccc ggatttccca
    11461 gaaaaggtgg tagaaagtgg gggattgtgg tagactaatt ttagttagtg gaggaatggg
    11521 gggctttcaa tgttgatggg tgaattccaa cataatattg atgcaaaagg acgtttaatc
    11581 gtgccctcaa aacttcgtga agaacttggc gaaaagtttg ttctgacacg cggattggat
    11641 ggctgtcttt tcggctatcc gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa
    11701 atgccgttgg ctaaaaaaga cgcacgtact tttgtgcgat tcttctattc ggctgccact
    11761 gaatgcgaac ttgataaaca aggacgaatc aatataccca gcacgttaag aaattatgca
    11821 gctttgacaa aaggttgcgt agtcatcggt gtatctaacc gaattgaaat ttgggatgag
    11881 acacgatggc aagatttttc tgcagcagca gaagagaact ttgacgaaat tgctgaaagt
    11941 atgattgatt tcggactata agaataggaa gaggctgact aatgactgaa acatttcaac
    12001 actatacagt catgttgaaa gaaacggtag atggattgaa catcaaaccg gatggcgttt
    12061 atgtagactg tacactcggc ggtgccggac atagtgaata tttattaagc caactaagtt
    12121 ctaaagggca tctgtatgcg tttgaccaag atcaaaaagc aatcgatcac gccaagctgc
    12181 gtttagcttc ttatgtcgaa caaggtatgg tcacatttat caaagcgaac ttcagagaat
    12241 tagaagaacg cttgagtgag catgtagata aggtagacgg cattttgtat gatctagggg
    12301 tttcctctcc tcagctagat gaagcagaac gtggatttag ttatcatcaa gatgcaccgt
    12361 tggatatgcg catggatcag tcagctcctt tatccgctta tcacgtaatt aatgaatatt
    12421 cctaccatga attagtaaag attttcttcc gctatggcga ggagaaattt tccaaacaaa
    12481 ttgctcgtga aatcgaacga gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg
    12541 tcgaaatcat taaacaagtc atcccagctc ccgcaagaag aaaaggcggg catcctgcaa
    12601 aaagaatttt tcaagctgtg cggattgcag taaatgacga actcggtgcc gaagaggcct
    12661 ctttagaaca ggcaatccgg ttattgaaag tcaatggaag aatcagcgta atcacattcc
    12721 attcattgga agacagaatt gtcaaaagta tgtttaaaga atatagtacg gtacaagatc
    12781 taccaccggg acttcctgtc atacctgacg aattccaacc agaactgaaa gtcattactc
    12841 gtaaaccaat ccttccttca gaggaagagc taacagaaaa caatcgatca cgcagtgcaa
    12901 agttacgtat tgcagaaaaa ataagaatga aagaggagta gaatgatggc agaattaaag
    12961 aaaatgcaag attacgaata tgatttacct gaattggtgg atcagccgga acaacaaatg
    13021 cctgattctt cttcgaatca agaaatcatt cttccacaat caccggcaaa acgattaaag
    13081 cagatttctg ctttggaaaa aattgctgtg gcaagcgtgg cgattgctat tgttgcttta
    13141 tgtgttttaa cagttatgct gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca
    13201 atacaaacag aaatcaatga taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac
    13261 gaactttctc gtactgaacg gattaaacaa atcgcagaag aaaaaggatt gtctattaat
    13321 gacgacaatt taaggaaagt gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg
    13381 gaaaagaaaa atctaaatcc gatgaacaat cgcaaaaaag taggtatcat tttatttgct
    13441 acgagtattg gattgttctt tttatttgcc gtccgtttct cgtatatcgt tattggaggt
    13501 catgttgctg gtacctcatt agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta
    13561 gtaaaagcaa aaagaggaac gatttatgat cgtaacggtg tggcgctcgc agaagatgcc
    13621 tcctcttatt caattaaagc gatactctca aaaacatata cctctgggga taaaaaactg
    13681 tatgttgaag aaaagaattt tgataaaata gcagaaatcc tgcataagaa tctttctata
    13741 gacaaaaaag atgcactgaa tattttagag gacggcgcaa aaaaagagct gtatcaagtt
    13801 gaatttggta gctatgggaa aaatatctcc caagaaacca agcaaaacat cgaagcggac
    13861 atgaaaaaag aaggtgtcgc tggtctttat tttgtcgacc atcaggctcg gatgtatcca
    13921 aatggcgtct tttcttctca ttttattgga tacgcagttc cagacaaaga tgagaatggc
    13981 ttagttggta aactaggttt ggagtctgct tacaatgata tcttgagcgg taaagatggt
    14041 aagattattt atcaaaaaga taattttcaa aatcctctgc caggtacagt ggcagaagaa
    14101 gaaaaagctg tcgacggaca agatatctac acgactttag acagcagact acaaagttat
    14161 ttagaaacac tgatggatca agtaaatgaa gaatatcagc cagaagaatt gacagcagtc
    14221 ctgatgaaag caaaaaccgg agaaatcttg gcaatgggac aacgacctac tttcaatcct
    14281 gaaacaatgg aaggattgac tggaaaagat gctatctggc gtaatttctt ggtacaagat
    14341 agttatgaac caggatcaac catgaaagtt ttcactactg cagctgctat cgaagaagga
    14401 gaattcaacg agaatgaaac cttccaatct ggaaaaatcc aagtagaaga tgctacgatc
    14461 aatgaccatg actttggtga aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca
    14521 agtaacgtcg ggatggtcat actggaacag cgattaggcg gacgatggta taactattta
    14581 caaaaactgg gatttggaca aagtactcat tctggattag atgatgaagt aaacggtgct
    14641 ttacctacat taaatattgt cgaccgagca atgagtgctt atggacaagc ggtaggtgtg
    14701 acgaatttcc aaatgatgaa agctttcact tctattgcga ataacggtac aatgattcaa
    14761 cctcgatata tcagcaaagt ggtggatccc caaactggag aagagcgaac aactcaaaca
    14821 gaagttctag ggcaaccttt ttcaaaagaa acaacagaaa aagtccgtga atatatgaga
    14881 gatgttgtcg aaagcgaaaa ctacggtagt gcttatggtg tatatagtgt acctggatac
    14941 aatgtatcgg caaaaaacgg aacagcccaa atcgcttctg atacaggggg atatcaaaca
    15001 ggagatacag cctacctgta ttcgattgtg gagatggttc cttctgaaga tccagactat
    15061 gtcttgtatc taacaatgaa acaccctaag acatatgatc gaatggcatt ggctaaaatc
    15121 gcaaatccat tgatgaaacg ggcaatggat tttaaagaaa ctgaagaaga ttcagataca
    15181 gaaacaaaaa cagaaaaagt atccgtagcc gattatcgta atcttgaagc ggacgttgca
    15241 gcagctgatg ctcaaaaaag tggactgcaa cctgtagtga tcggtaatgg taaaaaagta
    15301 caaaaacaat ctactgccaa cggtgatcag ttgatctctg gagaaaaact gatcctatat
    15361 actggtggtg ataagctgat gcctgacgta acaggatggt caaaagcaga tatcatgaag
    15421 ctggggaaaa tcctcggtat tgaagttagt tttgatggag atggttattg tgtcaaacaa
    15481 gaacttgctc catatgaaaa aatcactgaa gacaaactta gttttacttt agaagaataa
    15541 gaagagaaat aggagagaaa agagcatgga atggacacaa gcattgattc ccattgtcag
    15601 cagttgtgca atgacaattg ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa
    15661 gcaaggtcag gcgattcgtg aggagggacc aaagtggcac aatagcaaag ccggaactcc
    15721 tacaatgggc ggcttagtgt ttctgatcgg ctctattttg acaggaattt gggttggagc
    15781 ttggcaaaaa cagctaactc ctactctttt catcttgctt tttgtattag cgttatacgg
    15841 agtaatcggc tttcttgatg attttataaa aatattcaag aaaagaaaca tgggactgaa
    15901 ctcgaagcag aaattgattg gacagattct agggggaatc atcttttatc ttgtttaccg
    15961 ttctgaaggt tatccaggtg tattgaattt cttcggacta gatctacctt taggaatcat
    16021 ttatggtatt ttcgctctct tctggcttgt tggattttca aatgcagtaa atctgacaga
    16081 tggcatcgat ggattagttg ctggcttagg aagtatctct tttgcagctt acggtctaat
    16141 tgcttggcat cagcatcagt atgatgtatt aatcatttgc ttaagtgttt taggaggttt
    16201 actgggtttc ttcgtctaca acagaaaacc tgccaagatt tttatgggag atgtcggttc
    16261 attagcgttg ggcggacttt tagcggcaat ttctataatg ctaaaccaag aatggacatt
    16321 gcttttgatt ggtttgatct atgttatgga aacagcaagt gtgatgcttc aagttacttc
    16381 ctttaagctt acaggaaaac gaatttttaa aatgtctcca attcatcatc actttgaaat
    16441 gtgcggatgg tcggagtgga agattgatac agttttttgg ttgatcagca ttgtgacttc
    16501 gttgatcaca ttatggatcg tttggtagga gatgacggga aatgaaaaaa atatcaacat
    16561 atgaaaataa gaaagtgctt gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat
    16621 tattacatga attgggcgca cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc
    16681 cggaagcaca agagctgctt tctttaggga taaaagtaat aacaggtagt catccaatcg
    16741 aattgttgga tgaagaattt tcattgatgg tcaaaaatcc agggattccc tacagtcatc
    16801 ctttagtagc aaaagcccaa gaaatgggta ttccggtaat tacggaagtt gagctagctt
    16861 acgaagtagc ggaatgccca attattggga ttacaggaac gaacggaaaa acaacgacaa
    16921 caacgatgac aggactacta ctgaatgcag gagcagatca aggaattgca cgtttagcgg
    16981 gtaatattgg gtatccagca agtggtgtag cgcaagaagc aaaatcagaa gataaaattg
    17041 tgatggaatt gtctagcttc caactcatgg gaatcacaga tttccgccct catatagcgg
    17101 tgattaccaa tatctatgaa gcacatattg attatcatgg tacaagaaaa gaatatgtca
    17161 aagctaaatg gaaccttcaa aaaaacatga cagaaaaaga ttatctgatc cttaactgga
    17221 atcagtcaga actacaagaa ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga
    17281 caaaagaagt gttggaagac ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag
    17341 aaaaaataat ggaaatatct gaacttggcg taccaggtaa gcataatgta gaaaatgcac
    17401 tagctgctat ttctgtagca aaattgtatg gtatatcaaa tgaagctata cgagaaacgt
    17461 tgcatttttt ccatggcgta ccacatcgta cgcaatatgt aggagaaatc caaggcagaa
    17521 aattttataa tgattcaaaa gcaaccaata ttttagcgac taaaatggct ctaagtggat
    17581 ttgaaacgtc taaggtcgtg ttattggctg gcggattaga cagaggaaat acatttgatg
    17641 aattaattcc ttcattaaaa ggaatcaaag caatggtcgt atttggccaa acaaaagaaa
    17701 agttgatgga tgctggaaaa aaagcaggca tcgagacaat agtaacagcc gattctgtag
    17761 aacaagcagt tcctttagca ttagaaaaca gtacagatgg tgatgtagtt ttattatcac
    17821 cagcgaatgc cagctgggat caatacccaa attttgaaac tagaggaaac agattcatgg
    17881 aagctgtgaa ccgattaaaa tagaaaaagt agaagcgagc gataaaaaat gaaaattttg
    17941 gttacaggcg gaggaacagg gggacatatt tatccagctc ttgcctttgt caattatgta
    18001 aagactcaag aaccgaatgc tgaattcatg tatgtgggag caaaacgagg tctagaaaac
    18061 aaaatcgtcc ctgatacagg aatgcctttc cataccttgg agattcaagg atttaaacga
    18121 aaaatttcca tgcataacat caagacggtc cgattgtttc ttaagagtat ccgtcaagca
    18181 aaaaaaatcc tcagtgattt tcaaccagat atcgttatcg gtactggagg atatgtttcc
    18241 ggagcggtag tgtacgctgc agccaaaatg ggtattccaa cagttatcca tgaacaaaac
    18301 agtgtaccag gtgttaccaa taaattttta agccgatatg tggatcggat cgctctttca
    18361 tttaaagatg cggcttcctt ttttcctgaa aacaaagcag ttcttgtagg taatcctcga
    18421 gcacaagaag tagcagatac taaaaaatca gatattttga aaacatttga tttggatcca
    18481 gaaaagaaaa cagtcttgat ttttggcggt agtcaaggag cacttaaaat caatcaagca
    18541 gtatcagttt ttttaagtga atatattcct tcttcttacc aagttttgta tgcttcagga
    18601 gatagatatt atgaagagat cctaaataaa ataaatcctg aaatacaaaa tattagtgtc
    18661 cagccttata tcaaaaatat ggcagaggtc atggcttcca gtgatctgct ggtggggcgt
    18721 gctggtgcaa cttcgatcgc agaattcaca gctctggggt taccggcagt gttggttccc
    18781 agcccatatg ttacgaatga tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga
    18841 gcagcaaaaa tgattgcaga taatgaacta acaggtgaat cactaagtca gacagtcaac
    18901 gaaatcatgg gtgatgaaga attgcaaaag caaatgtgcc gagcttctaa agaacaagga
    18961 atccctgatg cttcaaaacg tctttatgac ttagtaaaac aaataatcaa aaaataaagg
    19021 cggtggtctt agtgagtaag aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg
    19081 aaaacaattt gactccttgg caaaaggcta accgaaaata tctggcagaa caagagatga
    19141 aaaaccaaga gccaaaaaaa gaagacataa cattagatac acccctttca gattctgaag
    19201 aagaaaaaac ggttcaagag gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat
    19261 tttttgaaga gatcaagcaa aagaaaaacg gtggaccata taacggttct tttttgaacc
    19321 ggctgcccaa tttaaaaagt caacggcaca aagtactatt tcgtcggtta gctttgatca
    19381 tcactgtatt gggtataccg ctgatttttt tgatctatta tgtttctcct tatagcaaat
    19441 tacaagcagt tgcagtatca ggcaataaga cagtcaacag ccaagaaatc atctctgata
    19501 caaaattgtc attaggagaa aatgtttggg gacagtattt tcatcgatct acttatatcg
    19561 atcgccttaa aaaagcacag ccaagaatcg aaacagcaaa tatacatttc aaagggatga
    19621 atgaattcga tttggatgta acagaatata aagagattgc tttgatcgca aaaaacaatc
    19681 aatactaccc agtgattgaa aatggaacag tattagatga aaaagtagca aatcctacaa
    19741 aaaatctccc tatcttggaa gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc
    19801 aatacaatca gttatcatca gaattacaaa aagcaatttc tgaaatcaag tacacgccaa
    19861 gagcaagcaa taaaaatctg atccaattga atatgaatga cggcaatcaa gttattgtaa
    19921 atatcaataa tcttgcgaac caaatgaaat attattcgca agtagccaaa gatatggatg
    19981 aaaaaggtgt gatcgacatg gaagttggta tcttctcgca tccttacggt agcactcaag
    20041 aaacggaaga atcgggccaa tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa
    20101 caaacgaatc aagtgaagag gcagaaacgc aaaaagcaca aacacctgga tcgtctagca
    20161 caaacggatc atctgattca aatgattcaa gtgaaacaag tcaaatagaa gataatccaa
    20221 gtacaactcc gtcaagttta aatgaaaatt gataaaacaa ctttaaaaag taagaatgta
    20281 ctttctaaaa accagtaatt tatggtagaa ttggaagcag tgaatatatc tattaataag
    20341 tatattttaa aacggacgct agttgttcgc ttgtatatgg aatagtaagt aggagggaat
    20401 cccattcatg gcaaaaacag gaatgtatgt aggccttgat attggaacga catctgttaa
    20461 agttgtcgtg gctgaatata tcgacagcca aatgaatatt attggagtag gaaacgcaaa
    20521 atcagaaggg attaaccgag gcattatcgt tgatatcgat aaaacggtcc aagcgataca
    20581 acgagcagta cgacaagcag aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg
    20641 tttgccagca aacatgcttg aagtagaaaa ttgccaggga atgattgcag ttaacggaga
    20701 ttcaaaggaa atcaccgatg aagatgtcag aaatgtggct tcagctgccc ttgttcgttc
    20761 aatacctcct gaacgtcaaa tcgtgtcaat attgccacaa gattttactg tcgatgggtt
    20821 cgagggaatc aaagatcctc gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt
    20881 gtttacagga cctaaaacga tcatccataa catacgtaaa tgcgtggaaa atgccggatt
    20941 aatcgtcaac gaaatggtga ttactccttt agcgctgact gaatcaattc tttctgacgg
    21001 agaaaaagat tttggtacga tcgtcattga tatgggtggc ggccaaacaa caacggctgt
    21061 tatgcatgat aagcaattga agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac
    21121 taaagatatc tctattgtat taaatacgtc atttaacaat gcagaagcgt tgaaaataaa
    21181 ctacggggat gcatacccag aacggacatc accagatgaa gaatttccag tagatgtgat
    21241 tggtcagtca gaaccagtga aagtcgatga acgctacttg tcagaaatta tttcagctcg
    21301 aatggaacag attttcaaca aagcaaaaga tgcattggac caaatcgaag cattagagtt
    21361 gccaggtggc gtcgtattga caggcggagc tgctagtctt cctggtgttg tggacttggc
    21421 acaagaaatc ttcggcgtaa atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa
    21481 cccggtattt acaaatgtaa taagtatcgt ggattattca gcaaacttga gtgaagtgta
    21541 tcaattggca aaaattgctg ttacgggtga aacctcagca gctcgtcaaa tggtagttga
    21601 acaagaaaca acaaacacat atgagagcta tgaagcacct gaagaaacaa tctacgatga
    21661 gccggaacca aaagagtccg gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa
    21721 tatttttgat taattcatcc tgggaggaat aagatatgga attctcaatt gataataata
    21781 tcaatgacgg cgcagtaatc aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa
    21841 accgaatgat tgaagaaaac gttaaaggtg ttgaattcat tacagccaac acagacgtgc
    21901 aagcattaaa aaattcaaaa gctgaaacag ttatccaatt aggccccaaa tatactcgtg
    21961 gtttaggtgc tggttctcag ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat
    22021 ccttacgcga agctttagac ggtgcagata tgattttcat cactgcagga atgggtggag
    22081 gaaccggtac aggtgctgca ccaatcgttg caggtatcgc gaaagaattg ggcgcattga
    22141 ctgttggtgt cgtgacacgt ccattcactt ttgaaggacc gaaacgtggc cgttttgctg
    22201 cagaaggtat cgctcgtttg aaagaaaatg tggatacatt attaattatc tcaaataacc
    22261 gtttattaga agttgtagat aaaaagacac cgatgcttga agctttccgt gaagcagata
    22321 atgttttacg acaaggtgta caaggtatct ctgatttgat cacagcgcca ggttacgtta
    22381 accttgactt tgctgacgtg aaaacagtga tggaaaacca aggtacagca ttgatgggga
    22441 tcggtgtggc tagtggagaa gatcgcgtag tagaagcaac gaaaaaagca atctcctctc
    22501 cattgcttga aacatctatt gatggagctg aacaagtact tcttaacatc acaggcggtt
    22561 tggatatgac attgttcgaa gcacaagatg cttcagatat cgtagcaaat gcagcaactg
    22621 gcgatgtaaa tatcattcta ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg
    22681 tgacggtcat cgcaacaggt atcgatgaat caaaaaagga acgcaagtct agccgaccag
    22741 ctagacaagc acaaatgcaa tcacctgctc aaaagactgt attagatatg gatcaagcta
    22801 aaccaacttc ttcagaagaa gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa
    22861 atgttcgtcc tagagttgat gattccaatt ttgacaatat cgaaaaaaaa gaatttgata
    22921 cattcaaccg cgaagaaaca aaatctcatg gcgatgatga attaaatacg ccaccgtttt
    22981 tccgtcgtaa aaaataaagg agaggaagag cacatgattg ctgacaactt gcagaaaatc
    23041 aagcaagaaa ttcaggattc gtgtgctctt gttcatcgtg atccgaaaga agttacatta
    23101 gtagctgtaa caaaatctgt tgaatccgct caagctgaag aattggttag agctggcgtt
    23161 atgaattgtg cagaaaaccg agtagataaa ttattagaaa agaaacaaga tttatttgat
    23221 tttcctgaaa taaagtggca tttaattggt aatttacagc gcagaaaggt aaaattgatt
    23281 gtaaatgaaa ttgattactt tcatgcgttg gacagtttaa gattagcaga agaaatccag
    23341 aagcggttag agaaagaatt atcctgtttt attgaagtaa atgtttctgg tgaggaatca
    23401 aaacatggaa tttcaccaga tgagctttta ccgtttgtgg aatctttagc tgcctgcaca
    23461 aaaatcaagg ttgttgggtt gatgactatg gcgcctaaag aagcttctga tgagcaaatc
    23521 cataaagtgt ttggtacgct gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc
    23581 catgcaccat gtagtgaatt aagtatgggg atgagtcaag actttcaaat tgcgattgaa
    23641 gaaggtgcaa catttatccg tgtaggaaca gcgctattca aagacgaata aaggaggaac
    23701 aaaaatgccg cttttaaaca gaggagcaat tgctaatttt ttcggattag ctgatgagga
    23761 agagtacgaa taccaagatt atcctgaaac aacaaaaaag gctgtacagc caactaaaaa
    23821 aaatacaaca cagaatcaat caactcattc tgtatctgct gcgaatactc atgaaaaaca
    23881 aaaaacggcg acttcacgtc ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa
    23941 agtaattgaa ctacatcaat cacctacaaa aactgtagcg aaagaaagaa cagcttcaag
    24001 aacctctaca caacctaaag caaatcttgc tggaaaaatc actgtaatgg aaccaagagc
    24061 ttattctgaa gctatggcga ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa
    24121 ttttcattta gtagaagaaa aacaagcgag aagaatcgtt gattttctaa caggaacagt
    24181 ttatgcacta gatggagata tcaaacgtgt tggagatgag atttttcttt gtacaccgac
    24241 tgggatggag attgacagtt caaccgctca atcttttgca gatgcgaatt tgtttgatct
    24301 gtaagaggag gaactacgat ttatactatt gcactattac ttcatttact tcaacgggca
    24361 gcctatttct atagcattat cttgatggta tatgcattgc tctcttggtt tccaggcggg
    24421 tatcaatcag ggctaggacg attattagct aagatctgcg aaccgtatat cagtttgttc
    24481 gatcgtctgc ctttgaggat tggacccttg gatttcacaa ttttaggggc aatcctgatc
    24541 ttgaacttag ccgtacaagg tttagcacag atcgttatat ggataactgt ccatttagtt
    24601 tattgattga agcaacaaaa tctttattct cagaatagat aggaggggaa aattttggat
    24661 gcaaatgtat accagcactt tcgggcagat gaacgtacat ttatcgattc ggtaggcgat
    24721 tggattgaac aggttcaagg acaatatgca ccctatctga ccgaatttct tgacccaaga
    24781 caatcctata ttttagaaac actgattcga caaagcagtg atttgcgttt catgttttat
    24841 ggcggatatg aacaggcaga aagaaaaaga tgtttgattt tccccgatta ttacgaacct
    24901 gtagaggaag attttgaaac agcattgtat gaaatccatt atcctagcaa atttgccagc
    24961 ctaagtcatg ggaaaatcct tggaacttta gtgggaacgg ggatcaaacg ctcttacttt
    25021 ggcgatatca tctcggacgg tgatcgctgg caagtattta ttgcaaatga ggtggatcat
    25081 tttgttgctg cacaagtaac aaagattgga aaggttgctg tacgcttgga accaatcaaa
    25141 tacacagaaa tgattatgcc gaaagatagc tggacacaag aaagaggaac agttagttcc
    25201 atgcgactag acagtgtgat ctctgagatt tataatattt ccagacagcg gtccaagcag
    25261 ctgatcgaag ctggtaaagt aaaagtgaac tggacagaaa atacaagacc tgattttgta
    25321 cttgaactat tagatattat ttcagtcaga ggttatggca gagtccagat ccaagaactt
    25381 gaggggaaga cgaaaaaaga aaagtacagg gtactgttcg gtgtattacg taaataaaat
    25441 aaaaaggtgg tttactcatg gctttaactc cattagatat ccaaaataag acattcccga
    25501 caaaaatgcg gggatataat caagacgaag tagatgattt cttagattta gttgttcgtg
    25561 actatgagga attgactcaa agaaatcgag aattagaaaa agcagtcaaa cattctgaag
    25621 aaaaacttga gtacttcaat gaattaaaag atgctttgaa ccaatcaatc atcgtagctc
    25681 aagatacggc tgataaagta aaaacaagtg caagcaagga atccgaagtg atcgttactt
    25741 ctgcacaaaa caaagcagat gagttagttg cgaacgctga aaaacgtgca catcaattaa
    25801 caactgatgc agaagaaaaa gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac
    25861 agcttgcgac agaaactgaa gatcttaaga aaaaaacacg agtattccat caacgtatca
    25921 gcttgatgct tgagtctcaa ttagaacaag tgaaaagtcc ggaatgggat gaaattttac
    25981 aaccattctc cagctacgtg actgacagcc atgaagtgat caaagaagtt ttagcaaaac
    26041 agcttgacaa tgaaaatgat actgatgtaa actcagacac aggagtcata gaagcaccta
    26101 tgacggcatt tgatacacag gctattgata tctcgatcgt gcctggtgaa aatgaaaacg
    26161 agtagaacag aaaagcactt tgttattctt aaagcgagcc gatgagagtg gaagttcggt
    26221 aagacactgc atggtgaaag atcctctact tgagacctgc ttttccgaaa ttagagtaag
    26281 aagcagcggc tgacctcgtt attggtcaca gagagtttgc actagataga tgcaactaaa
    26341 tttgggtggt accacggcat gttcgtccct tgcggctgaa catgcttttt tgtgtccgga
    26401 aaacaaataa aggagtgtat ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa
    26461 caaaattccc tatgcgtggt aatttgccaa accgagaagg cgagtggcaa aaagaatggg
    26521 aagaaaatcg aatctaccaa aaaagacaag agttgaacga aggaaaacct agctttgttc
    26581 ttcatgatgg acctccatat gccaatggta atatccattt aggacattca ttgaataaaa
    26641 tcagtaaaga cattatcatt cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc
    26701 caggatggga tacacatgga ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac
    26761 gtaaagaaat gtccatggca gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag
    26821 tagataagca acgagcagat ttcaaacgcc taggagttgc aggagattgg gaacatcctt
    26881 atgttacatt agatccttct tatgaagctg cacaagtacg cgtatttggt aaaatggctg
    26941 aaaaaggcta tatttacaaa ggattgaaac caatttactg gtctccgtca agtgagtctt
    27001 ctttagcgga agctgaaatt gaatataaag atgtaaaatc tccatctatt tttgtagctt
    27061 tcaatgtcaa agacggcaaa gatattcttg atgaagatac agcttttgtc atttggacga
    27121 caacaccatg gacattgcca gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg
    27181 tattggtgga agccgatggc cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc
    27241 aatcagaaat cggttgggaa aatgtaacag ttctaaaaga atttgctgga caagaaatgg
    27301 aatatatgac tgcaagtcat ccattctatg aacgtgaatc acttgtgatt ttaggcgatc
    27361 atgttacttt agacgctggt actggattag tccatactgc tccaggacat ggggaagatg
    27421 actatatcgc tggtagcaaa tataaactat ctgtagtgtc acctgtagac tcaaaaggat
    27481 tgtttacaga agaagcacct ggatttgaag gtatctttta tgacaaagca aatcctatga
    27541 tcactgatct attaaaagaa aaaggagctt tgctgaagct agatttcttt actcatagtt
    27601 atcctcacga ttggcggacg aaaaagcctg tgatctatcg ggcaactcca caatggtttg
    27661 catctatcga taaattccgt cagaatattt tggatgaagt agaaaaagta gagtgggtta
    27721 tcccatgggg taaaacacgt ttgtataata tgatccgtga ccgcggcgat tgggtaattt
    27781 cccgtcagcg tgcatgggga gtaccacttc cgattttcta tgctgaaaat ggagaagcaa
    27841 tcatcacacc agaaacaatc gaacatgtgg caaaattatt tgctgaacat ggatcaatta
    27901 tctggtttga aaaagaagcg aaagaacttc ttcctgaagg atttacccac ccaggttctc
    27961 ctaatggcga atttacaaaa gaaaaagata tcatggatgt ctggtttgat tctggttctt
    28021 ctcatgaagc tgttttacgt cagcgaccag aattatcatt cccagcagat atgtatttag
    28081 aaggatcaga ccaatatcgt ggctggttca actcaagtat cacgactagt gtggctatca
    28141 atggtgtagc accatacaaa tcagttattt ctcaaggatt tgctttagat ggtgaaggcc
    28201 gtaagatgag taaatcttta ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg
    28261 gtgcagatat ccttcgctta tgggtatcta gtgtagatta tgaagcagat gttcgtgttt
    28321 ctatggatat cttaagtcaa gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt
    28381 tcttattagc gaatacagaa gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt
    28441 tacgttctgt agacaaatat atgaccgttc gattgaacca agtaatcaaa gaaatccgag
    28501 aagaagggta cgacaaatat aacttcatgc atatttaccg tacagttatg aacttcttga
    28561 cggttgatct atcgtcattc tacctggatt ttgctaaaga tgtagtttat attgaagcag
    28621 aagatgatta tcaacgtcgt tgcatgcaaa ctgttttcta tcaaacagct gtagctttga
    28681 caaaattatt gactcctatc attccgcata cagcagaaga aatctggtca ttcctgaaag
    28741 aagaagagga gtacgttcag ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag
    28801 aagaattgat ggatacctgg actgctttca tggacttccg tgataatgtc ttaaaagcat
    28861 tggaagaagc aagaaacagc aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt
    28921 atccaaaaca acaagttcgt gaaatgttaa aagctttgga tgcagatatt gcacaattat
    28981 tgatcgtttc accagactac tttgaagtta tggcagcaga tgaacaagtg ccagataacg
    29041 ctatggtatt tgatgatgtt gctattacag tggaaaaagc agacggggaa acttgcgatc
    29101 gctgtcgcca agttcgtaaa gatgtaggag tagatgaaaa acttcctcat ctatgcggtc
    29161 gttgtgcaaa aatcgttgaa gacttctatc ctgaagctgt agctgaagga ttcgaagaaa
    29221 agtaaaaggg aaaagggggt gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg
    29281 tttaattttc cacgttcacg ataccagtta tctggttgcc agatccattc gaatccgtct
    29341 ttttcaagta gttcgaaagc tgctttaggt cccattgatc cagctttgta gttgggaaaa
    29401 tccggttgtg tttggtccca agcttttcga atgacatcca cgatatgcca agattgagcg
    29461 acttcttccc aatgagagaa gttcgttcca tcaccattca acgcatctaa tagtaatttt
    29521 tcgtaagctt ctgggctatt ttcaaccatt tctgcactat ttctaaattc caatttgatt
    29581 ggttccgttt caaatccttg ccctacttct ttcccattca aggataaaga aaatccttca
    29641 gttggctgaa tatagatcgt tagaacatta ggtggaagtg tcgtatcgtc acatggttca
    29701 tcgacagatg ttttgaatac attaacagga acttgtttga atacgatgtt gatccgagtc
    29761 cctttttcag tcaaacgttt gcctgtacgt acatagaaag gaacacctga ccaacggaag
    29821 ttatcgatag tgaatttgcc tgcgacgaat gtttcagtct ctgattgagc atccactttg
    29881 tcttcttcgc gatagccctt gaattgtaca ccatccaatt caccttgagc atattgcccg
    29941 cgaacgaagt tttctaacac ttcttcttcg ttataaatgc ggatagcttt caatgctttg
    30001 atcttttctg tacggatttc tttttcggaa aatgctactg gcggttccat tgcaagtaat
    30061 gctacaacct gtaaaatatg attttgcacc atgtctttaa gtgcgccact atgatcgtag
    30121 tagcccccgc gatcttctac accgaggttt tcgccaaaag taatttggat attgtcgata
    30181 tagcgattgt tccattgagc ctcaaaaata ttattggcaa aacggatagc tgagatgttt
    30241 tggatcattt cttttcctaa ataatgatca atcctgaaaa tatcttgttc tgggaatact
    30301 tctctgattt gctggttcaa ttcaaaagca gactggtaat cagaaccgaa cggtttttct
    30361 atgatcaaac gattgaagcc attttttgtc ataatatttt gtgatttcaa atgagaaacg
    30421 atcgttccaa aaaattgagg tgccatagcc aaataataga cacggttacc gtttaattga
    30481 tagtgtccat ctaatcggtc agacaattct tttaatgtct gataatgcgt tgaatcattt
    30541 acatcatggg attgatagta aaaatggctt gcaaaggtag ttgcttcttc atctgaagga
    30601 ttcaatgcac ggattgtttc tttgacaact tcacggtagt gctcatcgct ccagggacgg
    30661 cgagcggttc cgattacagc aaaatgctcg cctaaatttc cttttctgta aaggcggaac
    30721 aaagacgggt acaatttacg ctgggctagg tcacctgtac cgccgaatat cgtaaacaat
    30781 acattttttt cattagacat gtggatacac tcctttatat tttatcgaca aaaatcgtgc
    30841 tggccgcctt aaagctgacg gggattttct ttctatcatt ttggatagtg atcggtcctt
    30901 cataagctgc gatgtcaacg atctcatatt cttcgttgat attcaggtca atagtgacta
    30961 agtaatccaa caattctttt tcatcaagca ctcgtgcgat acgcactttt gtaccgacag
    31021 gataggactc caatgtttgt cggcgttctt catggacaac ttcctctttt ttaggaatcg
    31081 atcctccatg aggacaatgt tcaggatgat tcaaatagtt ttctaaatga tctgcaagat
    31141 gttccgaagt cacatgttcc aatacttcag catcatcatg gacttcattc cagctatagt
    31201 ctaaatgttc aactaaaaaa acttcccata agcgatgttt gcgaatcaga ctgctagctt
    31261 tttgcagtcc gcttgcagtc aattgtacac cttgataagg ggaatgttct acaagctctt
    31321 ctttcacaag tttagaaatc atttcactga cagatgcagc tgagacatct agactagata
    31381 cgatttgttt attgttgact ttcgttgtat ctccacctag ttctaaaatg attttcaaat
    31441 aatcttctcg atttggtgtc atattcgatc atcctttcat ggtaaaaaac catcattagt
    31501 aagtgtaaca aaaaaaacgt taattgacta tttttctaaa taaaaaacaa aggattttta
    31561 gacgttttta caacaaaaaa cgaaagatga tagagaagac tcattctttc gtttataaag
    31621 gatatatata tacattggga gggatctatt tttcagataa caacttatat gaagtgtctg
    31681 cagattcgtc ataaagagtt tgttcgggat aagaaaagat aagataaaaa ccatactgat
    31741 cacgtacgac taaacgatat tcatctattt ctaccacaga tgtgccaaga gactgaccgt
    31801 tgatcaaaaa cgtgtaatca gatgtaatct gtaattgttg tttttgctgg ctatcactaa
    31861 agaaccatgt accttgatac tttgagagtc cttctgactt ttccgcttgt tttttctttt
    31921 tcaagtaaat aaaacttgag agaacggtaa tgagtgaacc aagaaaatag atcatttttt
    31981 ttatcaccaa atcactcctt tatgagaaaa taatcgcata gacatattct ttcttcttta
    32041 tttcattgta ccgaataaaa gctaaaattt ctctaagact ttcgactggt ttttttactg
    32101 tttttctaca aattatgata ggatacatag taggaagaaa ggtgacataa tttgaagtat
    32161 cagcaagtgg aaatagcttt ttttattgac cgcccgaatc ggtttatagc acattgtatg
    32221 aaaaaagacg gagaaataat tattgcgcat gtcaaaaata caggtagagg aaaagaggtc
    32281 ctgataccag gcgctgaagt ggctattgta tttgctcctg gacctaaacg gaaaaccgcc
    32341 tatgatctga ttgctgtaaa aaagaagggt gactggttta atatcgatag tcaattgcct
    32401 aatcggctgg caattgatgg tattttagac ggcacgatcc ttttaccagg attaactggt
    32461 ccatttacgg tatacaagcg tgaagtaact tatcttcact ccaaatttga tatttattta
    32521 gagacggtgg tcggacaaaa agcatttgtt gaagtcaaag gtatgacact tgaaaataaa
    32581 gcgattggtg cttttcctga tgcgcccaca ctcagaggat tgaaacatat aggagaatta
    32641 acgtatgccg cacaagatgg ttatgccgct tatgtcttgt ttattgctca gttcgaacat
    32701 cttcatctgg caacgatcca tgaagaaatg caaccagcac tagctgatat ggttcgacat
    32761 gcgcagcaaa gcggagttca aatcctcgcc tataattgtc aagtatcggc agatgaggta
    32821 actgtcaagc aggccattcc atttgatgta gatatccctt ttgaagatcc tatacaatag
    32881 aaagaaggta aaataatgat caacttaggt attatcggaa caaattggat tactcatcaa
    32941 tttgtccaag cagctttaag cacaaaaaaa tatgaattga cagcggtata ttctagacat
    33001 ttggaaaaag cacaggaatt tggttctcat tatgaagggg atatcgaatt cgcaacagat
    33061 ttgaagactt ttttcaatat tactcatatg gacaccattt atattgcttc tccgaatagt
    33121 cttcacttta gtcaagctaa gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag
    33181 ccagcttttt cgacgccaaa agaaatggat gaaatcatcc atttggcaaa tgaacacaat
    33241 gtgtattttt ttgaagcagc aagaaatatc catgaatcag gttttaaaaa gattgccgat
    33301 tacttaccat taaaagacga gataatcggt gcaaatttca gctatatgaa atattcttcg
    33361 cgctatgacc aagtattaga tggagaagaa ccgaatatct tctcgactca tttctcaggt
    33421 ggtgcattga tggatttagg tgtttacctt gtatatgcag ctgttgcttg gtttggaatg
    33481 ccaaaagaag tccattattt cccaagaaag atttctactg gtgtagatgg tcttggatgg
    33541 gggatcttac gttatgagaa ttttgatgta tcgatccagc caggtaaaat cggtgattct
    33601 tatttaccgt cagagattta ttttgactca ggaacgctag tgatgaatgg agtcaatgca
    33661 attgaacaag cacaatttca tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca
    33721 gctgcagaaa atccaatggc agatgaagca gaggattttg ccaatgtcat ggctcatccg
    33781 aaagatccag cttggggatt gttgtatgaa gagtggatcg agctagctag aaatgtgaac
    33841 caagtgattt atgatttaag aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa
    33901 gatttttaat gaacgaattt gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg
    33961 ataaagggtt tatctcacct tctcttattc aacgagcagt ttttcagcca ctaagtaata
    34021 aacaaagtat agtagcggtt tctcctacag gttcaggtaa aacactggct tatctttggc
    34081 ctctactact aaacgtggaa ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc
    34141 aagaactagc tattcaagta gcagatgttg cacgtgaatg gggaaaagac aaagagttga
    34201 aagtccaatc tttggtgggc ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa
    34261 aaccagaaat cttgattggt acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa
    34321 taaaagcaca tcaagtgaaa acaatggtat ttgatgaagc agaccagcta tttgacgctg
    34381 ggaacagcca aatcattgat caaattcttc atcaagctcc aacagagtat cagctagctt
    34441 ttttttcagc gacagcagat cgttcccttg aaagcattga aaaaatcact gggaaaacgt
    34501 ttgagacaat tgacgtgaca gcagaggacg attctcgaaa aggactgaga cattactttt
    34561 tacgtgtacc gatacgaaag aaagatgact acttgagacg tctcacgcat atagaagatt
    34621 ttcatggtct gattttcttt aatcaactga ctgaattagg cgtaatggaa gaaaaattgc
    34681 tgtatagagg tgtaccggta ggaagtctgg cctctgatca gaacaagcta ctccgaaaag
    34741 cagcgctaga acaattcaaa aagaaaatgg tcagagcact tttgaccaca gatgtggcgg
    34801 ctagaggttt agatattaca ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg
    34861 aagaagctta tttgcatcgt tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa
    34921 ttacactcgt acaagataat aatctaagag atttaaaacg gcttttacgt ccatcaaata
    34981 taacacttga agagattttt ttatacggag gtaagctgca aactgagcag ccaaccaaag
    35041 aagatgcagc tcctgcagta aataaaaagt atgcgaaaac tttttctgga aaggagcaga
    35101 acccagaata taacaaagaa aaaataagaa aaaaagttaa aaataaatca aaaaaagaga
    35161 gaaataaagg agcaaggcgc aaataagcgc ctttttatgc tatgattatg aaaacgggaa
    35221 caaaattggt tgccagagga ggggttatgt gaaagaacgt caagaagaga tcttgcagct
    35281 attgacagaa aaccaacaag gattgacagc aagtgatgta gctgagcgct taactatcga
    35341 tcgaagcaat gcaagccgat atttaagtga actatacaag gcccatcata ttgtaaaaac
    35401 cgctggccga ccagtcgttt actctttgcc aacggaaaag tcaaaatctg atgaagtgca
    35461 tgtcgactct tctacgcaag tgacttttga aacactcgtt ggagaaaatg attctttaaa
    35521 agtcagcata cagcaggcaa aagctgcgat tctttatcca ccgcgggggt tgcatacgat
    35581 tatttttgga gaaactggta cagggaagtc gatgtttgct gaatgtatgt atcattttgc
    35641 aatcgattca gagatgcttt ctgcagatgc gccatttgtt tctttcaatt gtgcggatta
    35701 tgctcaaaat ccgcaactgc tctttggaca catctttggg atcaaaaaag gggcttacac
    35761 aggagcagct caagatagtc ccggtctaat agccaaagca gacggcggta tattgttttt
    35821 agatgagatc caccgtttac cgccggaagg gcaggaaatg ctgtttactt tcattgataa
    35881 aggtatttac cgtccgcttg gagaaagtgc acaagtacat gaagcatctg ttcagatcat
    35941 tggggcgacg actgaatctt cagaaagttt cttatctacg ttcaatcgac ggattcctat
    36001 ggcaatcacc ttgccgaatc ttgcttcacg ttcactggat gagcgttatg aaatcatctc
    36061 tctttttatc aaacaagaag cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc
    36121 gattcttgcc ttcatgcttt atgatgcgga aggaaatatc ggtcaagtga aacgagactt
    36181 gaaactagtc tgtgcaaaat cttttttaca ttaccgtact catcatgaga aaaagctaat
    36241 cattcgaaaa gaagaacttt cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat
    36301 accggaacgt ctcgaccgat tcatggacag caaaagtcag tatttgacat ttgaaccagg
    36361 atttgctgat gttgtctggt cacaagatcc agaacgaaac atgcaggtct ataacgatat
    36421 tgaggaaaaa gtcacttctc tttccgaaac cggtgtggag aacattgatc tagagaccct
    36481 catttcaaaa gatgtggatg cttatttcca gacatatgtc aaagaattga ctaaaagttc
    36541 gattcccaaa gatcttttgc cagatgaaat ctggcggtta accaatcaat tgtatgatgt
    36601 ggctgaaaaa cggttagacc gtatttataa tgaaaaagct cgttttgctt ttgctttgca
    36661 tctgcaaagt acattggatc gtgtgagaga aggtcatatg atcgtccacc cagatttgaa
    36721 taacgtacgg aaaaatttta agagggaatt ccaggtagca atcgatcttt caacgatcat
    36781 tgaagaagaa gaacaagttg agattccttt tgatgaaatc ggctttatta gtatgtttct
    36841 ttctatcaat ctaggagaaa gtgagcctgt taaagaaaac aaagtagatg ttctcatatt
    36901 gatgcacggc agacagactg cttcaagcat gttggagatg gcacaagaac tattaggaac
    36961 aaaagtaggg aaagctttta atatgccact ggttatggaa gttcaaacaa tgtatgaaga
    37021 agtgttacaa tatgtcaaaa actatcaaga gcatttgacg aatggcctac tcttgttaac
    37081 agatatggga tcattaaaca ccttcagtga tttgatttat gaagagactg gcattcggac
    37141 gaaagctatc acgatgacaa gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt
    37201 tggaagaagc ttagaagata tttatcaaaa tatccagctt tcctttgaat cgatcgtacg
    37261 tgatcagttc aaagcggaaa agtctgaaac tttacagcgt aaaaaagccg taatcgtgac
    37321 atgctttact ggtgaaggag tagctgccaa attatatcag cgtattatgc cagtgattaa
    37381 tcaaaataag gtagaactga tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca
    37441 catcgatgcc ttattggaag aatatgagat caaagcaatt gcgggaacag ttgatgttga
    37501 ttatcaaaat atcccatttt tttcagccta tgacgttttt gatgatgaaa aactaaatat
    37561 tttaaagcgt atagctgatg atgagatccc gacagctaaa attgtcgatt cacttgctgg
    37621 atcattaagc gatgtctttt ctattgatga actgatccgt tcattgcaaa agacagttca
    37681 gcagatccag accgatttgt cgattatcgt ggaacctgga gtagatactg gaatcgttat
    37741 ccatttagcc ttcatgttag acggattgat caaacaagaa agccatcggc aatttgagaa
    37801 actgtctatt ttctcaaaaa tttatcgttt ggaaatggat atcgttcggg caaacttgat
    37861 ggcactagaa cgaaaatatc aggtaaaaat tccagaagat gatatcgcat ttcttacaca
    37921 aatgtttctt gaaaataaaa taaaaaaatg aacacactaa tcatactgtt tacacactaa
    37981 acatctgtgt gcgcactata aaaaagagct aaaaagcatg aaaacgctat ttcagctctt
    38041 ttttttattg gcatgcttct tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa
    38101 aaatggcaaa aaaaacaatc atgttagttt gttcagcagg gatgagtacg agtttattgg
    38161 tcacaaaaat gcaaaaagca gcagaagaaa aagggatgga agccgatatt tttgcggtct
    38221 ctgcctcaga tgcagataat aatttagaat ctaagaatgt cgatgtatta ttattaggtc
    38281 ctcaagtacg tttcatgaaa gctcaatttg aacaaaaact tgcgcctaaa ggtattccat
    38341 tagatgtcat caatatgcaa gactatggca tgatgaacgg agaaaaagtt cttgcacaag
    38401 cagaaaatct aatgaaataa atcaagtatg cagttggggg tataacaacg tggaagatca
    38461 acaaaatcta gaagccatca tggggttaat catgtttggc ggtaatgcaa agagcgatgc
    38521 aatggaagcg attgcggctg ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc
    38581 tgatgcagaa gaatcactta tacaagcgca tcatgcacaa acagacatgt tgacacaaga
    38641 agcacaagga aatcatatgc aagtgacatt attgactgtt cacagtcaag atcacttaat
    38701 gacatctatt gcgtttactg atttagcaaa agaaatcatc gaactttatc gtcgcgtata
    38761 cagcgaataa atacaacttc ttttttgaac ggaagaagaa aaatttttga acggattttt
    38821 tattttgtta taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg
    38881 aacggactat caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg
    38941 caaaaacatt tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg
    39001 ggatcagtcg ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt
    39061 acctatgctg acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc
    39121 ggaatcaatg gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt
    39181 tcttgggggt ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt
    39241 ggtttagcta ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat
    39301 gtgaatgttc cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt
    39361 gcagatggca cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga
    39421 aacgcttatt ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg
    39481 atgcttgcca acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca
    39541 tttgcagcca ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc
    39601 gtaggtcgtg tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagag
    39661 ccattcctag gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa
    39721 gtattctggt tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc
    39781 tggggacaag cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact
    39841 ggtacaccag ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga
    39901 tcgttcgatg cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct
    39961 atcttgattt tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct
    40021 ggtatcttca atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc
    40081 atgtttatcc cattcttgat tgctccagta gtagcaacag caattggatg gacagctact
    40141 tatcttggcc tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta
    40201 ttgtcattct tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg
    40261 gtcgtgactt tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc
    40321 gcaggcgaac aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat
    40381 aatgaatttt cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga
    40441 aatcaaagaa ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa
    40501 acaaggaatc aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa
    40561 agcaaaggaa ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc
    40621 tgtttagaaa atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt
    40681 atgtagtatt ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga
    40741 tttgtatagt atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa
    40801 ggggaaaaaa agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta
    40861 aatattccag catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt
    40921 aaagcgaccg gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa
    40981 ttctcgcaaa ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat
    41041 aagtttcgta catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg
    41101 gacgattcga cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa
    41161 aagttgaatg atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa
    41221 aaaatgctag atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt
    41281 attgaaaaat gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa
    41341 tcctcactag cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag
    41401 gtgtatcttc gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata
    41461 cacctgaaag gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt
    41521 ttgatgagga ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt
    41581 acggacaaga gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg
    41641 gacaaggcgg gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag
    41701 taagcttatc ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac
    41761 aatcatttga taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa
    41821 aatctggaaa aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt
    41881 atgaacagat gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt
    41941 ataaattcaa gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag
    42001 caacgggata tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat
    42061 ttttggatca tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt
    42121 ttgaaacatt tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa
    42181 atccatttgc ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt
    42241 tgtttttgga aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag
    42301 aagatcatta tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa
    42361 atcatctctc aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata
    42421 tcacgcagct attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac
    42481 aggtgacaaa agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc
    42541 ttaatgacta tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt
    42601 ttttcatttg gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa
    42661 ttttttgttt tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt
    42721 aatcttttcc cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg
    42781 cttatcattc attggaaagg acctccctac attatttcat tctacctcta gtttatcatc
    42841 tgttaaggac tagtggaaac gttttgccta acttataagc tgattttttt agcagataag
    42901 cttgcaagta aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag
    42961 taggatagag caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt
    43021 ataaataacc cctgtatcct ttgtggtaca ggggtttgtt ttgtatttgt gccaaaaatg
    43081 tgccaaacta gttatcattt ttatataaac tatcgaatac agcattgatt ttttctttat
    43141 cctgatcttc aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc
    43201 tatgtcctaa ccgttttgat acgctaagta tatttactcc ttgataaagt aaaatagatg
    43261 catgtgtatg ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt
    43321 ttttgcgcaa atatttgctg acggcatttg atgaaactaa tccatttttt aagttgaaaa
    43381 aaacaaaatt atgagggtt
//
LOCUS       DAZKAS010000009        41222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000009 DAZKAS010000000
VERSION     DAZKAS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.009"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..662)
                     /locus_tag="ACG8H7_000380"
     CDS             complement(192..662)
                     /locus_tag="ACG8H7_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFP8982515.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="ACG8H7_000381"
     CDS             992..2632
                     /locus_tag="ACG8H7_000381"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFP8982516.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="ACG8H7_000382"
     CDS             2786..4108
                     /locus_tag="ACG8H7_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFP8982517.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="ACG8H7_000383"
     CDS             complement(4176..4610)
                     /locus_tag="ACG8H7_000383"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8982518.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5512)
                     /locus_tag="ACG8H7_000384"
                     /pseudo
     CDS             complement(4675..5512)
                     /locus_tag="ACG8H7_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(6254..6880)
                     /locus_tag="ACG8H7_000385"
     CDS             complement(6254..6880)
                     /locus_tag="ACG8H7_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982519.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7175..7510)
                     /locus_tag="ACG8H7_000386"
     CDS             complement(7175..7510)
                     /locus_tag="ACG8H7_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP8982520.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7497..7781)
                     /locus_tag="ACG8H7_000387"
     CDS             complement(7497..7781)
                     /locus_tag="ACG8H7_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP8982521.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7837..9360)
                     /locus_tag="ACG8H7_000388"
     CDS             complement(7837..9360)
                     /locus_tag="ACG8H7_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP8982522.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9353..10528)
                     /locus_tag="ACG8H7_000389"
     CDS             complement(9353..10528)
                     /locus_tag="ACG8H7_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP8982523.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10532..10717)
                     /locus_tag="ACG8H7_000390"
     CDS             complement(10532..10717)
                     /locus_tag="ACG8H7_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982524.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10683..12377)
                     /locus_tag="ACG8H7_000391"
     CDS             complement(10683..12377)
                     /locus_tag="ACG8H7_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP8982525.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12374..12847)
                     /locus_tag="ACG8H7_000392"
     CDS             complement(12374..12847)
                     /locus_tag="ACG8H7_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP8982526.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12916..13071)
                     /locus_tag="ACG8H7_000393"
     CDS             complement(12916..13071)
                     /locus_tag="ACG8H7_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFP8982527.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13205..13585)
                     /locus_tag="ACG8H7_000394"
     CDS             complement(13205..13585)
                     /locus_tag="ACG8H7_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP8982528.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13589..13804)
                     /locus_tag="ACG8H7_000395"
     CDS             complement(13589..13804)
                     /locus_tag="ACG8H7_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982529.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13807..14214)
                     /locus_tag="ACG8H7_000396"
     CDS             complement(13807..14214)
                     /locus_tag="ACG8H7_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFP8982530.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14480..15955)
                     /locus_tag="ACG8H7_000397"
     CDS             complement(14480..15955)
                     /locus_tag="ACG8H7_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFP8982531.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15945..16793)
                     /locus_tag="ACG8H7_000398"
     CDS             complement(15945..16793)
                     /locus_tag="ACG8H7_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFP8982532.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16830..17192)
                     /locus_tag="ACG8H7_000399"
     CDS             complement(16830..17192)
                     /locus_tag="ACG8H7_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982533.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17193..17351)
                     /locus_tag="ACG8H7_000400"
     CDS             complement(17193..17351)
                     /locus_tag="ACG8H7_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982534.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(17492..18116)
                     /locus_tag="ACG8H7_000401"
                     /pseudo
     CDS             complement(17492..18116)
                     /locus_tag="ACG8H7_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18113..18424)
                     /locus_tag="ACG8H7_000402"
     CDS             complement(18113..18424)
                     /locus_tag="ACG8H7_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982535.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18468..18761)
                     /locus_tag="ACG8H7_000403"
     CDS             complement(18468..18761)
                     /locus_tag="ACG8H7_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982536.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18955..19602
                     /locus_tag="ACG8H7_000404"
     CDS             18955..19602
                     /locus_tag="ACG8H7_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8982537.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19663..20808
                     /locus_tag="ACG8H7_000405"
     CDS             19663..20808
                     /locus_tag="ACG8H7_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8982538.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20889..21194)
                     /locus_tag="ACG8H7_000406"
     CDS             complement(20889..21194)
                     /locus_tag="ACG8H7_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFP8982539.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="ACG8H7_000407"
     CDS             complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="ACG8H7_000407"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP8982540.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21647..21913)
                     /locus_tag="ACG8H7_000408"
     CDS             complement(21647..21913)
                     /locus_tag="ACG8H7_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFP8982541.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(22168..22893)
                     /locus_tag="ACG8H7_000409"
     CDS             complement(22168..22893)
                     /locus_tag="ACG8H7_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP8982542.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(23254..23324)
                     /locus_tag="ACG8H7_000410"
     tRNA            complement(23254..23324)
                     /locus_tag="ACG8H7_000410"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23290..23292),aa:Gly,seq:ccc)
     gene            23441..24193
                     /locus_tag="ACG8H7_000411"
     CDS             23441..24193
                     /locus_tag="ACG8H7_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP8982543.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            24209..24688
                     /locus_tag="ACG8H7_000412"
     CDS             24209..24688
                     /locus_tag="ACG8H7_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFP8982544.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24939..26519)
                     /locus_tag="ACG8H7_000413"
     CDS             complement(24939..26519)
                     /locus_tag="ACG8H7_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP8982545.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(26512..27255)
                     /locus_tag="ACG8H7_000414"
     CDS             complement(26512..27255)
                     /locus_tag="ACG8H7_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982546.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(27431..28207)
                     /locus_tag="ACG8H7_000415"
     CDS             complement(27431..28207)
                     /locus_tag="ACG8H7_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP8982547.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(28455..29291)
                     /locus_tag="ACG8H7_000416"
     CDS             complement(28455..29291)
                     /locus_tag="ACG8H7_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFP8982548.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(29302..30231)
                     /locus_tag="ACG8H7_000417"
     CDS             complement(29302..30231)
                     /locus_tag="ACG8H7_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP8982549.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            30423..30776
                     /locus_tag="ACG8H7_000418"
     CDS             30423..30776
                     /locus_tag="ACG8H7_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP8982550.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(30850..31506)
                     /locus_tag="ACG8H7_000419"
     CDS             complement(30850..31506)
                     /locus_tag="ACG8H7_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP8982551.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            31732..33666
                     /locus_tag="ACG8H7_000420"
     CDS             31732..33666
                     /locus_tag="ACG8H7_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP8982552.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(33752..34975)
                     /locus_tag="ACG8H7_000421"
     CDS             complement(33752..34975)
                     /locus_tag="ACG8H7_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP8982553.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(34969..36840)
                     /locus_tag="ACG8H7_000422"
     CDS             complement(34969..36840)
                     /locus_tag="ACG8H7_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFP8982554.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(36861..37979)
                     /locus_tag="ACG8H7_000423"
     CDS             complement(36861..37979)
                     /locus_tag="ACG8H7_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP8982555.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(38062..38895)
                     /locus_tag="ACG8H7_000424"
     CDS             complement(38062..38895)
                     /locus_tag="ACG8H7_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982556.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(38888..39805)
                     /locus_tag="ACG8H7_000425"
     CDS             complement(38888..39805)
                     /locus_tag="ACG8H7_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982557.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(40032..41156)
                     /locus_tag="ACG8H7_000426"
     CDS             complement(40032..41156)
                     /locus_tag="ACG8H7_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8982558.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        12361 a         8301 c         6864 g        13696 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     5101 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5161 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5221 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5281 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5341 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5401 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5461 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5521 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5581 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5641 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5701 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5761 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
     5821 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
     5881 tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa caaatggcaa
     5941 aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct gattgaacat
     6001 cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt ttttatatct
     6061 tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgagaa acccatatca
     6121 aagaactata aaggttatca aaacagcgtt tatgactaaa tgaattagag ttatctatca
     6181 ctatttttca agcgatttaa accaaatggc atacaatatg gcatacaaaa acttcaataa
     6241 gccgtggggg tttttaaatt tcttcttgtt ctttaaaatc aatgagtgga gctacataat
     6301 ttgaatccac tttttcaaac gtcacttttt gcatcgacga atgttttctt tcccaagata
     6361 acgtaaatag atttgactct ggacttataa agtctacttc accttctagt attactttct
     6421 tatgcaaata ttcctctaat aaatatagac ttgctaaagc atacaacaca ttttccaaat
     6481 ttgcttcctt ataatttgaa gaacgaccat gttttactct gttatatatt ttccaccaat
     6541 ctggtgtatc ttcaggccac ccatctccaa aaggttttag tgaagtcaat gttaaagaat
     6601 tcataaccct aacaccatta tccaaacaca ttaattcgct agatttaata acagctttaa
     6661 aatcagttat gcttttattg ctactgctat caaatccgca aagttctttg aaaataatct
     6721 cacactccga accaatcgct agtagtagtt tttgatattc aatagagaat gttttgaaat
     6781 tattctcatc gagcatcaca aaatctgtgg tattaataaa ctcattttct aaatgtagat
     6841 aatacttcca ataaaaattt gtaaactctg ctttgtccat taaatcacct cttttgaatt
     6901 ataacaaaaa aaacacgtcc acaacagacg tgttctttga tttctacaca atcagcttgc
     6961 caaaaccatc caatcagtcc tagcatgcct gtattaaaat tgaacgttca tcatctatca
     7021 tctttcttag tccagcatgt ttgcaaccac acctacaatt ttcagtccaa caatagattt
     7081 cgactagcta agtcttgcaa aattcaggca gctaaccctt catttactac cttaattata
     7141 ctaaataccc acaacgttta caagtgtaat cattctaaga aatctgttta gctatgatcg
     7201 ttgtataatc tttaaacgcc gttccttcat tgattgaaat aattttataa ctcttgtttt
     7261 tccaagctac tttcatatcg tttgttagtt cataatcttg atcgtatcga ataataaacg
     7321 tcaacgttcc ttcaagaatg gtacctacac tagcaagcac gtcttttagc atttgtgaac
     7381 gcacattagc ccaacattca aatacagttt cttcactatc caccatctgc ccatcttcat
     7441 cttttacccg ttttgtcttt ttaaacttga ctctctcagt caattcattt acattcttaa
     7501 tgattggcat aataagcacc tctcatttgt tgtactaatg cttgtaccgt tactggtatc
     7561 cgttcgctgc tggcttcttg acggttcaaa taccaatgtt gagtaagtaa tgacaccgcc
     7621 aaatcaaatt gctgataatt atccatatct ttatccgttg cgctactatc aatagcactt
     7681 tttatatatt cgcctgctgt atcaatcaat tgttgaataa agtcatcatc aatagtgtga
     7741 tcgatccgca tacttttttt tatgctatcc aaaattacca ttttatctct cctaatgaaa
     7801 aaaaggcagt gggataaacc gccgcccaat atttttctat acttttggtg ttacaactgg
     7861 cgtaaactca atatacactg aagcattttg atcaattttc ttataatcgt tgcgaacgat
     7921 cactgcaagt ccttgtgagt agtaatcgaa tttttcccat tgagtcgtta cttgatttct
     7981 acgagcaaca aagatagatt gtgcaatatc ccccataatc atagggaatg ttccagcttt
     8041 aggattggca aacaatgtat ctgcaatcaa tactactggc ataccgaata atgatttacc
     8101 actaggtgct gtcacatcgg gttgtaaaat gtaacgtcca tctgaatctt tcaatgtatc
     8161 taggtggtaa tatccgcttt ggtttagaat taccatttta tttaatgctg ggtctaatgc
     8221 cacattgtat agttgtttca aatcgtccaa cgtagcagcc gttttcttag tgaatgtttt
     8281 taataaatcc atgatatgct tattgtcagt gttttctacc aatttcgcta actgatcttt
     8341 gacctcttgt acaatattca ctgctgaatc ttccacaacc tcatttgata aggcaatctt
     8401 accagcacga gtttctactt tataatcaac tgaagtaaac atttcagcgt caatatcacc
     8461 aatttcagca agttcagctt ttgttgctaa cactgcttgt tggttagttg ctactggata
     8521 tttaccttgt ccatttgata ctgtttttac tgttgcatat tgagctaagt tataatttga
     8581 acgtttcaaa tcaaagactt caccgattac ttcttcggga acaactgccg ctgcatttac
     8641 agtagtgact ccatcacgca cttcgccttg tgaacggata tattcttcat acccacgcaa
     8701 ttctgttttt tcgttatcga ttaaagtttt ttccatgtta ttactctcct tttgtgcttt
     8761 agaagtggat tctaagctat tgtttgtttg ttttgcttgg ttgttactca taaacgattc
     8821 gtaagaacgc ttatttactt ggacatttga tgaatcataa gcgggaacgg tcacgacact
     8881 aatttcatta agtgctttga ttttatctat tgatcgttca attgtgccat cttctttttt
     8941 gtcgaatgaa tctaatccaa gcacgaaccc aaaggacata gaatcaacaa ccccttttga
     9001 gatattctca tacacatcat ttgaataggt cgtgtcggtt agctctgctt caaaatgtaa
     9061 tcctgtatca tcgacattca atttcaacgt acctgctttg acactagcta gtggctttga
     9121 ataatcgtgt ccatataata agaacacatt tgataaatcc acctctttta atgcttcggg
     9181 tgtaatcact tctacaaagc cgcctaaatc ttttgacggt tgcccgaatt tcaacgcata
     9241 gcctgaaata gttttgccgt ctgttggttg tttttcattt tctttctcta ctttgggaga
     9301 atcggctgtt agctcagctt cttctgtcag ccgtttttcc ttttcttctt cattattcaa
     9361 ctggtgtaac tccttcctgt tcttttaatt gatatttctc caacgtatct aaatacgtaa
     9421 agttcaaact tgctagtaaa cgatcgccac catccatggg gggtagcccc attttcgacc
     9481 gcccctcatt aatcgtgaga agtgaccctt tgacctgatc caatacattt tttacttttg
     9541 tttcaggatc agtctctagt aactgatcgg aattaaagcg gatattggtt tcaagtttcg
     9601 tatctaattc actcacaaac acattaaagt aatgaatcag cgtactttgg atatactgga
     9661 gattactttg aacggtgcta gaatgttcat tttcaacgcc taaacgctca atcggtacac
     9721 caaacgcctt ggcaatttgt tttgtgttcc aatcattgga attgataagc ttcaaaacat
     9781 cggtgtttat ttctaatgtt ttgtagtcca ttgtttcatc aagaatgatc gttctaagag
     9841 cattatctcc actacttgaa ccgttggctt cttcaaactt ttcacgaatt gcgttctttg
     9901 ctgatccgtc taaatctgac ttatgaacct tcaaaatacc tgacccactg acaccacgag
     9961 taaagaaatt gtgcaatgtg cggttgccag cttgttgaat tttcagttca tcacgtaacg
    10021 cataaagtgg tggtaatcct gttaaaccat cttgggtgaa atatttgaag tgcaaaatat
    10081 cgctagactt cacacgtctt tttttatcac caatctgata agacaacgta ccattatcta
    10141 attgagtaac cgttacactt gagttaggta ataagtgaat ttcttctacc ttttcaccgt
    10201 tgcgtttaat ctctgcaaag ctattaccgt ttagcaacat attgaccgcc aaagcaaact
    10261 ttaaatgcca accatccatt tctgaattag gtttctcgtt tagtaacttc accagttcat
    10321 tatcagcttg tggcatattg tttttgacca attgaatcgg acttgaagca atatcactag
    10381 caataattcg aacggctgca aacacatcac tatttcttaa tgcacgaacg cttgtaaagc
    10441 tggtggtgta atcatccgat tggattgata ccacatgttg taggaaagga tctcccgttg
    10501 tattttgtct cgctttaaaa aatgccatct tttaacctcc cttctgttct gtatttagca
    10561 tgaccgctac cccaataagc actatgccca gcgctaacaa gccatagtag acatttgtta
    10621 gtaaggtgat agcaaccaac acacacacca atccaagaag taaaagaacg gtatgaatgt
    10681 aattagaaag aaaattcttc acttgtataa aattcattgt ctgctttcgc tccttccgtt
    10741 gtggtgaagt aatccattgc aaacacatag gcattgatta atgcagctat tggatcaatt
    10801 ttgttactgt tctttgattt attaatctga atgccgttgt tatccgtttt taaaatagcg
    10861 ttgttcactg catgggtgag aatcgtattt ttattgtgaa taacgttgcc ttcataaact
    10921 tgttctctaa aagttcgggt cggaacgttt agagtaatcg ttccttgtct tacttctaac
    10981 attgggtagt tggctttttc agctttactg ataagcgaat tagcgttata cgggtcataa
    11041 caaatcccca tacattccaa atcattttct tgaatcaaat cttgaataaa ctgaaatact
    11101 tggtcatagt ccacgattcc actttctaat tgagtaatgg aacactcacc agctttttct
    11161 agttctcgat aaggcaaacc gtcgcgcttt tctttgtctt gaagcccata tttggtggct
    11221 acaaagctat gtgaatcaca ataaagttcg ccgttatcta gcggtacgat ccatgaaacg
    11281 ctagttaaat catctgtttt agataaatcc actccgatat aaacgggctt gcctgtaata
    11341 tcttgtggtt ctacttcggt tgcctgccag tcttcagcaa tcatatagct gtcctcactc
    11401 gcttgtctcc ataaattgaa gtttttcacc aatacagaat taaggttatt ttgtttcaaa
    11461 gcaaggttca catcatcttg aatggttgga atcattactt ttttgatttc ttcactttca
    11521 aaaatcggat tggctttgat ccatttctct tgatcgtgaa tttcttcggg atcgtccaat
    11581 tcccatatcg ctataaagta cctgtcggct tgttcttcgc cttttagcac acgatctaac
    11641 aataagtatt cctcgtacat aggaacgttt aaatcaagcc cagcggtact aataacacac
    11701 aataagccgt tcttttgttg ggtcattcct gacttaatga cgttgtacgt tttacgagtt
    11761 tttgcttcgt gccactcgtc aagaattgcg gttgttggtg caaacccatc tagcgtgctg
    11821 gtttcactag ccagtgccat agcgaacgaa ttagagggca agtgctgaat cttagagttc
    11881 ataattttca gttgtggtct taaaaactta ctagacttga ccacgtttct aagtgaatta
    11941 gacagcatat catagcccaa tttagcttgt ttcaaagcgt tacttacaaa caatacttgc
    12001 cttgcttctg ctggttcttg ttccataatc aacgcattgg cagccatgcc acttgcgata
    12061 tacgttttcc cattcttacg gctcatactg gtaaacgctc gattgaatcg cctgtaattc
    12121 ccttctttag tacgccagcc ataaagactg cctagaatga atttttgaaa tcctaacatc
    12181 ttgattgctt taccgtccgt agatggtagc aactcaatga atttaatcgc tttggtggct
    12241 tgttcttcat cgaacacata agggaagcta tctgacttag aacgctccaa atcgcttata
    12301 tgacgctcac acgcttggat aattttcttg ggtgcaatca gcttacctga taggactttc
    12361 tcaatgtaat cattcatgaa ttcatcaact ctttgaacgg gtcttcgggt tcatcatttt
    12421 taaccttgtt caaagctagt tttgatcgtg attctaaggt catacctagt gaggtggctt
    12481 gttttttcaa atcactcata gcttgtgatt gaacccgaac cgctggattg gctttcttta
    12541 ctccgttttc gcatgtgata aacgtacctg attttcttat ctcggcttcg gctgttttca
    12601 ttcgagcaaa ggctaaacaa taggctacca atgaaccata atctgcttca ctcaatggat
    12661 aatctttttt taatgttggt acgatacgat tccattcact tctagcgctc gctgccatcc
    12721 agtcaggggg cgtgatattt attaatggtt gctgcttgaa caattcttct ctagcgtcca
    12781 cacggattgc tttttcttca cttgatatat tgccttttgt atcgtgtaat attttagggt
    12841 gtcccatgtt gtcctccttt cagacaaaag aaaaaggaac gtaagcaatt aagctcacat
    12901 tccttcgttt ttcgatcaga atttttcttt atctaattta tctatataaa tgatttttcc
    12961 gttctgcatt tgaatggtca cttgaccaaa atgaggtaac tcaatggtgc gctgtttccc
    13021 tgttttatca gtaataacaa ttgtattttt caataaatct cctactttca ttatatactt
    13081 tttcacttgt gattacaagt gtaatcgtta tttgtaatta cccttatgcc ttttcacaac
    13141 ggttatttgt accaaaatga gtaggcatcg attgtcagta agtttcacac tatgccccct
    13201 taactcaaag ggtactcttt tctcgtcttt gtctttcatt ggctgtcttg gaattatggt
    13261 gtgcatggca taaagaacga aaattagttt catctaaccg tctattccaa tcatctttta
    13321 gttcaatttt gtgatctaca acatcagctt tccttatcaa gccttcttct aggcaatcct
    13381 cacatactgg atgatttaac cgatacaagt atgaagcctt tcgccatgtt ctactttgat
    13441 agaattgaaa atatttacct tcgctttgct ttctttcttc acgcttaatt gccgtttgct
    13501 ttgctttatg cttcccacaa tacttctgcc tataatcaat caatgtttta catccagcat
    13561 gattacattg ttttttaact gccatagatt accaaccaaa gcgatctcgt tcgtcagaat
    13621 catcttgttc ccattttgat ttgtattcaa tataaggcga aactatcttc aagcacgttc
    13681 cgtctttaaa attaacttta gtgattttat cttcctcaaa actaataaag tctacatctt
    13741 cttgagtgtt tgaatactca atgtgtttct caccatcgaa gtatctaata ctttccatat
    13801 gcataattat tctacctcca ataaattagt tatcaattca tcatctgtta gtagcaaagc
    13861 caagaactta tcatggatgt ttaaaaacaa attatcctca ttcgatgtaa gctctcgttg
    13921 tctcgctcga tcatataggt taagcagaca attaaattca tccattagct cacctgtatc
    13981 atcatagttg ccactatcta ccaatgatcc cactactagc gattgacacc acgctactga
    14041 ctcctgcagc caatcaggtt cttcttccat tatctttaat acttctggat caaatgtgga
    14101 ataatcttct gttaccaatc ccacactaaa tgccagcgtg ttcattagaa accgatccat
    14161 ttcatctgta tattgaaagt tcggtgcttt gtttgttgaa ataatattga gcatttactt
    14221 tcctccttga ttcgtaacac gtggtaacac ctacgtaaca ggtacccgtt accactcaat
    14281 cccaacgggg acaagggttc tagcgttttg gtaacaggta acgggtaaaa tccagtaaca
    14341 ttattcccca gcatattagc taactatttc aaaagtttta aggaaataag gtgttacccc
    14401 gttaccaaat tgctgtatcc cttggggcag taagggtaaa ctagtaacgg gctaggtgtt
    14461 actgacccgt tactacccgt tactttttgt actttccagc tcgattttta ttttcatagg
    14521 catttgactt attgtaaaat cctcgtcgtt tatttctttt tggcagttgc ttccgttccc
    14581 aaccaggaat actgctaata atcaaggcaa tctttttagt ttctgcatct gatcgaccat
    14641 tcaataaatc ctttgcttgt ttatcaaaaa cagcttccaa aatatcagcg gtgagtacgc
    14701 tatcaactaa ataaaactct tgttgcatag tagctccatt atagaaatgc tgtaaatttt
    14761 tctcattaca tttactgtca ggatagttct gataataaga acgtctcgcc cacgctggag
    14821 cttcttccca tttatacggg atttccattt ccaaatattt agtgattgct tctttaattg
    14881 gattctccat cttggcatcc tcttgatagt cttttgcaat ttctaagact tctttatctt
    14941 catcgggact aaaatagatt cgttgtcctt tatcaaaaag cacctttgct tcagctaaca
    15001 cttgaagaaa atattcatcc tttgttttaa acggattcat ctttggctta ttcttacaag
    15061 gcaaaggata aaaacggcgg tttcctgtat catctttcaa atactgccca tcattcgacg
    15121 ttccaataaa tacacattgc cttgcccaag ctatgacatt ccgaccataa ggaggtctaa
    15181 ttttatcgat cttggcactg atgaagtttt ttatctgttc aattttagtt ttgttcattg
    15241 aagataattc tccaagttca ataattacat tgccttgaag ctgcatataa tcatctttct
    15301 tttcacctag cgaatctaat gaatcggtga aaaaatcatc cgtgtaaagt aatgctggaa
    15361 cggtactctt accaatccct tgcggtccat ccaaaactgg aacgatctca aactttattc
    15421 ctggtttata gatacgtgcg actgatccga ctatccaacg ttttgttact tctcgtgtgt
    15481 aaaggttgtc gtctacccct aaaaggtcaa taaacagtgt ttccagtcta ggctgttgat
    15541 cccatttaac tttttcgata cgttctttta ctgggttgaa aacattcttt ctagccacat
    15601 ttactactgc tttgtgtatt ttgttgtcat caaaaactac attgtagttt tcttcaatat
    15661 atgccgttaa ggcatcctca aaactgtctt cccattctcc agcggtacga ttgatccaag
    15721 gagaatcttt taataggtga atgtatccgc taaattcgtt ataagcaatt gttccagcta
    15781 aattgctatc attggttaaa atcaattctg cattcacaac actgttactt ttaatctgat
    15841 cgtctttatt gcatcttaat cgtgacatcc aggttttcaa ttcaatgata ttgtcttcac
    15901 gctcgctggc ttcgggaaag tctaaaatac tatctaattt atgcttatga gccatgagct
    15961 ttccccctca aatctttttt aatcatcgat cgaataattt gattcacttc tttatctttt
    16021 agcggtggtt cattgtacgc atttgctgta taaatcaatt ctgctaaagt ttgattatct
    16081 acaccgtgcc atagtaacca accgcataac ttagttaaat gatcgtttct actaccttcg
    16141 ccagcgcctt gaacaatcat atctaatttt tttgctgtcc ctgtttttgc tacgggtcgg
    16201 tattgagtct ttatttcttg cggttttttt agcagtaggt tcactagcca atcgggagct
    16261 ggtgcaatat tatttagaga tacgttttca ataggttcat atgctctccc aacacttctt
    16321 gaaataatca tagttggcgc tattggaact cctttagtga taatatcaat cccgcttaaa
    16381 cttttacctt tatcatcacg cccgaagata tcagcaaatg cacctgtttc ttgttggata
    16441 attacaccat caggaatttt aaagaagaaa tggattcctt tacctggtgt gatttccgta
    16501 taagtagaag gaaatggatc atacattgaa gcaagttttt tataattctc aacgccgttt
    16561 cgtgtttcag aatgttgatc taggtcaact accaccattt gatgatcttc aagcattaac
    16621 ccaatatttc tagttgcatc ttccgtccac cattcattaa tttgattaga atcgttagtt
    16681 gcatcattcc aaccactaga gccttttatt ggaattttag ttccaggaga taagggataa
    16741 attgaccatc ccgacttttg taattccgtt gctgcttgat aattatttgc caataaacct
    16801 ctcctccttt ttcaaaatac tggtataaat cattcagaca cctccatgag cttcaactgc
    16861 tcaagttcgt atttcaaccc atcccgaaca aattcagcac cttttaattg attctgatac
    16921 tttacccatg tattaaatgt tttcaccctt gcttcttgca tatcagtaat gtttaaccat
    16981 gcggttaaaa tctgattttt tagctcgttg atttctcgac aaattatttt gtgttctgca
    17041 tgttttttga gtacatacaa aactgttagt ttttcattga atgaatttcc aaagtaggtt
    17101 ttcccgatat tattttctag ttcagcgatc ccttgattaa tttcctctaa ttcaaggcta
    17161 tcgagttggt ttaatagttt tgttttgtac atctacttat gccctccagt tcttaattga
    17221 tagtcgtact cgtcataagc agtgaagaga tataacgcta atcctgcaaa gatagctatt
    17281 tttgcaatta atggtaataa gccgaaaatt ccgccgatta aaatgcttcc tataattaag
    17341 tttcgtttca taatggttcc ctccgatttt ttaatatggt ataatcggaa caaagaatat
    17401 cgggttttat cccaattaag ttgcctttat cctgtccgcc aagactgtta tggataaggc
    17461 tttttctttg ttctcgatca tgttaatgct cctaagttgt ctgcaattca gcagaacgat
    17521 tataaatttt ctcactcact gaaaactgca acccatattt ttctttaaca gtcattaatt
    17581 ctactgtctc atctaaaata ggttcacgat caaccaacat ttcaggagtc atatctttct
    17641 tttttaccat tttggaatga ccataaatag ttgatacagc tttattggct atcacattcg
    17701 ctttaatgta gtctactggt tttggttcag taatagcgtg gcttaattta gtcattgctt
    17761 ctttttggtg ttctttgtct agcatgcgga atacttgaaa gccttcaagt cctgtggact
    17821 ggcgtagttc tttgatgatg tcgaaaatcc agtcttgaaa tgcctcggct tcttttttac
    17881 ggcttttgaa tgccatgcgg taaatatttt ttttcgttga tgatgtttac atcttgtgtt
    17941 ctgcccaatg aatcgatgac cttactagta gtaaccccat ctttaggcaa agagtgaatg
    18001 gctctagtaa cctgtttaag acctaacgct tccgcaacat ctttcgccac tgcccaccat
    18061 tcatcattaa tatcgacaaa tcttataatg tgtccgttcc aaatttcagt tttcattcaa
    18121 cgtcctccag ttcaagccct agcaagctcg ctaacaactc aacttgctga tacaaaattt
    18181 cttgatactc ttcaatagaa agtcgttctt taaactctgc ttgtactaaa tcgttcaagc
    18241 cacgcaatac tccgcttacc gttggttctt gtggtagcaa tttttgatag tccatttgtt
    18301 cgtacaaaca ttcaaacgtt tcttctagcg ctttgccttc caagtttgct aattctgccg
    18361 ctgtgtcttc tgtaagaatt gttgataata ctttttcgat tgctttattt tgtgtcgatt
    18421 ccattattat ttcctcctat taatcccttt gcccccacgg cttgcgttta tttttggtat
    18481 tcttccataa agcgatcaca ttcatcttgt gaaattctta cgactccatc aactataatc
    18541 aactttaaac cgttaggaac atatttctgc atcatagtat tgtatgaaac gttcatatac
    18601 ttagcagctt gtgccttgtt catatatttt ggaaactggt actttttttc taataatgaa
    18661 agtaacgttc cttgagtttg ctctacttgt tgaacaactc gatcgtcaat aattttaaaa
    18721 gctggatcat caagcgtgag tttgtcaatt tttaattcca tatttatccc ccctcgttag
    18781 catacatttc tatacaaaga ttttaaaaaa atgcaagagg ctcttgaata catttctata
    18841 cctcttataa actcaatata ccatcatacc ttttcatttg caagaatttt aacatacttt
    18901 tttgaacttt tatgtttcac acatatttaa atcgtgatat agtagagaaa atatatgaat
    18961 ggtggtgaaa atgtggctag aaaaaaatac cccgaagcag tggagacagg acaaagaatt
    19021 agactggcta gaattgaaaa gggactctca atggaacaac ttggaggtaa attatcccca
    19081 ccggccagta aaggtgccgt tagtaattgg gaaaatggct ataatcttcc caataacacc
    19141 aggcttaaac aactttcaaa ggtattagat gtatccacta cctatctatt aaacggatct
    19201 tatacattaa gagatatgca tatgatgccc gaaattaagc gaaatgtgct tgaaaagcaa
    19261 ttgaaaaaaa ctattgaaaa aaataactct ataattgatt ctaatctttc taatgctatc
    19321 aataacatcg aagaagataa cctgactgtc tcacagaaga tttatttaac aaacgcaata
    19381 aaatatctta aaacaggatc tcgaattgaa tttcctgacg ggagtacccc aattgcttat
    19441 ctcgcagctc ttctaaatac gcttttacga tatgactcat taattaatga ttcagaaaaa
    19501 aatacagatg agaaaaaaga gtatcaagaa ttttatgaaa atgatcttct aaaagagttc
    19561 acagctcttg ttactgagct aaataaacac tattcaaaat aataataaac tgcctcctcc
    19621 aaccgccccc acggcttgct acactatgga gggaaaacta ttatggcaac attcaagcaa
    19681 tatgaaacta aaaaaggtaa attttggctc tatgaagcct atcttggaat gaacaaaatg
    19741 actggcaaac ctgataaggt tcgtagacga ggttacaaaa caaaaaaaga agcccagctt
    19801 gccttgtcac gcttgcaagt ggactacgat aaaaacggta ttaaaaaagc gaacaacgaa
    19861 acgtttaaag aagtttatga cttgtggcta gaaacatata aaaccactgt ccgagaagcc
    19921 accttcatga aaactgaaat caagtttaga aagtggattc ttccaaaata tggaaatctt
    19981 cgtgtgaatg aagtcacggt aaaaaaagcc caacaaattg taaatagctg ggctgaaact
    20041 actgaccaat acaaggtact tcattcaact tctaagcgta tttttagcta cgctatcaat
    20101 cttgggatta ttgagactaa tccactagac aacgtattaa tgcctaaaag aaccaccact
    20161 aagcataaag aaaaggtcaa agtttacagt aaagaacaac tgatcacctt gtttgattac
    20221 ctaaacagca aacctgaaac ttatcgaaac gactttgata aaacgttact acgctttctc
    20281 ttttatagcg gggttaggat cagtgaagcc cttgcattga attggtcaga cattgatttt
    20341 caagaaaata cggtcaccat caataaaaca ctaagccaat ccaaaaatgg ctataaaatc
    20401 agtgatccta aaactgattg tagtgctggg gttcttccac tagatgaaag aacaatttct
    20461 atattaaaaa aatggcagat taatcagcgg aaatatatgc tgacacttgg tgtaactgat
    20521 ccgactatga ttttttgtgg tatctataaa gaactaatca cacaccacgc tatttatgca
    20581 agattgatta ctatttgtga aaaagctatg gttccatttc tcggtgtgca tgtagcaaga
    20641 cacactcatg cttcaatgct cttggacgct ggagctacta tggtagaagt tcaaactaga
    20701 ttacggcata caaaaattag tcagacgatc gattgttacg gtcacttggc aaaagaaacg
    20761 aaagaaaaga ctgttgaaaa attagtacaa cacttaaata ttgactagat ggcatacaat
    20821 atggcataca gtaaaaaata aaaaaaggag aaaccctatc atatcaaggt ttctcctagc
    20881 tttatagtct attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
    20941 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
    21001 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
    21061 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
    21121 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
    21181 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
    21241 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
    21301 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
    21361 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
    21421 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
    21481 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
    21541 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
    21601 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
    21661 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
    21721 gaatcaaatt tcgagcatca cgataacgag gaatgtaggc cgggtctcca gaaagcaagt
    21781 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
    21841 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
    21901 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
    21961 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
    22021 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
    22081 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
    22141 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
    22201 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
    22261 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
    22321 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
    22381 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
    22441 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
    22501 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
    22561 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
    22621 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
    22681 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
    22741 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
    22801 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
    22861 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
    22921 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
    22981 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
    23041 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
    23101 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
    23161 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
    23221 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
    23281 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
    23341 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
    23401 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
    23461 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
    23521 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
    23581 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
    23641 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
    23701 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
    23761 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
    23821 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
    23881 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
    23941 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
    24001 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
    24061 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
    24121 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
    24181 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
    24241 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
    24301 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
    24361 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
    24421 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
    24481 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
    24541 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
    24601 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
    24661 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
    24721 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
    24781 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
    24841 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
    24901 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
    24961 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
    25021 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
    25081 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
    25141 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
    25201 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
    25261 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
    25321 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
    25381 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
    25441 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
    25501 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
    25561 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
    25621 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
    25681 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
    25741 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
    25801 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
    25861 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
    25921 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
    25981 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
    26041 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
    26101 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
    26161 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
    26221 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
    26281 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
    26341 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
    26401 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
    26461 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
    26521 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
    26581 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
    26641 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
    26701 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
    26761 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
    26821 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
    26881 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
    26941 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
    27001 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
    27061 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
    27121 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
    27181 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
    27241 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
    27301 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
    27361 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
    27421 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
    27481 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
    27541 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
    27601 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
    27661 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
    27721 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
    27781 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
    27841 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
    27901 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
    27961 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
    28021 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
    28081 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
    28141 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
    28201 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
    28261 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
    28321 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
    28381 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
    28441 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
    28501 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
    28561 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
    28621 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
    28681 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
    28741 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
    28801 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
    28861 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
    28921 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
    28981 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
    29041 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
    29101 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
    29161 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
    29221 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
    29281 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
    29341 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
    29401 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
    29461 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
    29521 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
    29581 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
    29641 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
    29701 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
    29761 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
    29821 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
    29881 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
    29941 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
    30001 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
    30061 agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg
    30121 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
    30181 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
    30241 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
    30301 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
    30361 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
    30421 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
    30481 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
    30541 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
    30601 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
    30661 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
    30721 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
    30781 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
    30841 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
    30901 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
    30961 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
    31021 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
    31081 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
    31141 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
    31201 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
    31261 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
    31321 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
    31381 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
    31441 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
    31501 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
    31561 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
    31621 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
    31681 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
    31741 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
    31801 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
    31861 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
    31921 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
    31981 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
    32041 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
    32101 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
    32161 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
    32221 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
    32281 gacattttga ttctagatga accaacgaac catttagata tcgagacact ttcctggctt
    32341 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
    32401 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
    32461 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
    32521 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
    32581 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
    32641 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
    32701 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
    32761 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
    32821 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
    32881 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
    32941 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
    33001 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
    33061 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
    33121 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    33181 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    33241 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    33301 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    33361 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    33421 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    33481 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    33541 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    33601 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    33661 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    33721 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    33781 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    33841 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    33901 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    33961 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    34021 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    34081 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    34141 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    34201 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    34261 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    34321 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    34381 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    34441 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    34501 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    34561 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    34621 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    34681 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    34741 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    34801 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    34861 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    34921 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    34981 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    35041 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    35101 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    35161 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    35221 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    35281 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    35341 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    35401 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    35461 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    35521 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    35581 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    35641 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    35701 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    35761 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    35821 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    35881 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    35941 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    36001 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    36061 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    36121 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    36181 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    36241 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    36301 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    36361 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    36421 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    36481 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    36541 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    36601 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    36661 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    36721 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    36781 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    36841 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    36901 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    36961 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    37021 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    37081 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    37141 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    37201 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    37261 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    37321 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    37381 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    37441 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    37501 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    37561 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    37621 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    37681 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    37741 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    37801 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    37861 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    37921 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    37981 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    38041 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    38101 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    38161 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    38221 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    38281 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    38341 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    38401 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    38461 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    38521 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    38581 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    38641 agctaaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    38701 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    38761 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    38821 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    38881 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    38941 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    39001 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    39061 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    39121 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    39181 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    39241 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    39301 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    39361 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    39421 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    39481 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    39541 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    39601 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    39661 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    39721 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    39781 tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt
    39841 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    39901 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    39961 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    40021 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    40081 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    40141 aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt
    40201 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    40261 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    40321 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    40381 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    40441 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    40501 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    40561 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    40621 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    40681 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    40741 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    40801 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    40861 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    40921 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    40981 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    41041 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    41101 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    41161 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    41221 tt
//
LOCUS       DAZKAS010000010        40995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000010 DAZKAS010000000
VERSION     DAZKAS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.010"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1422
                     /locus_tag="ACG8H7_000427"
     CDS             82..1422
                     /locus_tag="ACG8H7_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8982559.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1640..2083
                     /locus_tag="ACG8H7_000428"
     CDS             1640..2083
                     /locus_tag="ACG8H7_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP8982560.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2587..4005)
                     /gene="nhaC"
                     /locus_tag="ACG8H7_000429"
     CDS             complement(2587..4005)
                     /gene="nhaC"
                     /locus_tag="ACG8H7_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP8982561.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4408..5631)
                     /locus_tag="ACG8H7_000430"
     CDS             complement(4408..5631)
                     /locus_tag="ACG8H7_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP8982562.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5662..6501)
                     /locus_tag="ACG8H7_000431"
     CDS             complement(5662..6501)
                     /locus_tag="ACG8H7_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP8982563.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6518..7198)
                     /locus_tag="ACG8H7_000432"
     CDS             complement(6518..7198)
                     /locus_tag="ACG8H7_000432"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP8982564.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7200..8273)
                     /locus_tag="ACG8H7_000433"
     CDS             complement(7200..8273)
                     /locus_tag="ACG8H7_000433"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP8982565.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8329..8567)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8610..8843)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8920..9591)
                     /locus_tag="ACG8H7_000434"
     CDS             complement(8920..9591)
                     /locus_tag="ACG8H7_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8982566.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9678..12326)
                     /gene="mgtA"
                     /locus_tag="ACG8H7_000435"
     CDS             complement(9678..12326)
                     /gene="mgtA"
                     /locus_tag="ACG8H7_000435"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP8982567.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12668..12842)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13015..13452
                     /gene="ndk"
                     /locus_tag="ACG8H7_000436"
     CDS             13015..13452
                     /gene="ndk"
                     /locus_tag="ACG8H7_000436"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP8982568.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13474..14340)
                     /locus_tag="ACG8H7_000437"
     CDS             complement(13474..14340)
                     /locus_tag="ACG8H7_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP8982569.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14500..16593)
                     /locus_tag="ACG8H7_000438"
     CDS             complement(14500..16593)
                     /locus_tag="ACG8H7_000438"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP8982570.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17342..17965)
                     /locus_tag="ACG8H7_000439"
     CDS             complement(17342..17965)
                     /locus_tag="ACG8H7_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982571.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18124..19521)
                     /locus_tag="ACG8H7_000440"
     CDS             complement(18124..19521)
                     /locus_tag="ACG8H7_000440"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8982572.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19553..20524)
                     /locus_tag="ACG8H7_000441"
     CDS             complement(19553..20524)
                     /locus_tag="ACG8H7_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8982573.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20898..21323
                     /locus_tag="ACG8H7_000442"
     CDS             20898..21323
                     /locus_tag="ACG8H7_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982574.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21811..22512)
                     /locus_tag="ACG8H7_000443"
     CDS             complement(21811..22512)
                     /locus_tag="ACG8H7_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP8982575.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22528..22968)
                     /locus_tag="ACG8H7_000444"
     CDS             complement(22528..22968)
                     /locus_tag="ACG8H7_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP8982576.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22968..23543)
                     /locus_tag="ACG8H7_000445"
     CDS             complement(22968..23543)
                     /locus_tag="ACG8H7_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFP8982577.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            23784..23993
                     /locus_tag="ACG8H7_000446"
     CDS             23784..23993
                     /locus_tag="ACG8H7_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFP8982578.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(24391..24903)
                     /locus_tag="ACG8H7_000447"
                     /pseudo
     CDS             complement(24391..24903)
                     /locus_tag="ACG8H7_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(25029..25730)
                     /locus_tag="ACG8H7_000448"
     CDS             complement(25029..25730)
                     /locus_tag="ACG8H7_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8982579.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25838..27281)
                     /locus_tag="ACG8H7_000449"
                     /pseudo
     CDS             complement(25838..27281)
                     /locus_tag="ACG8H7_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(27295..29160)
                     /locus_tag="ACG8H7_000450"
     CDS             complement(27295..29160)
                     /locus_tag="ACG8H7_000450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP8982580.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29281..30132)
                     /gene="licT"
                     /locus_tag="ACG8H7_000451"
     CDS             complement(29281..30132)
                     /gene="licT"
                     /locus_tag="ACG8H7_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP8982581.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(30442..31155)
                     /locus_tag="ACG8H7_000452"
     CDS             complement(30442..31155)
                     /locus_tag="ACG8H7_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFP8982582.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31223..31549)
                     /locus_tag="ACG8H7_000453"
     CDS             complement(31223..31549)
                     /locus_tag="ACG8H7_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP8982583.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31672..31899)
                     /locus_tag="ACG8H7_000454"
     CDS             complement(31672..31899)
                     /locus_tag="ACG8H7_000454"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP8982584.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32221..33474)
                     /locus_tag="ACG8H7_000455"
     CDS             complement(32221..33474)
                     /locus_tag="ACG8H7_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP8982585.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33535..34275
                     /locus_tag="ACG8H7_000456"
     CDS             33535..34275
                     /locus_tag="ACG8H7_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP8982586.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34361..35119)
                     /locus_tag="ACG8H7_000457"
     CDS             complement(34361..35119)
                     /locus_tag="ACG8H7_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP8982587.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35116..35580)
                     /locus_tag="ACG8H7_000458"
                     /pseudo
     CDS             complement(35116..35580)
                     /locus_tag="ACG8H7_000458"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(35599..36096)
                     /locus_tag="ACG8H7_000459"
     CDS             complement(35599..36096)
                     /locus_tag="ACG8H7_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFP8982588.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(36164..39331)
                     /locus_tag="ACG8H7_000460"
     CDS             complement(36164..39331)
                     /locus_tag="ACG8H7_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP8982589.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39555..40439)
                     /locus_tag="ACG8H7_000461"
     CDS             complement(39555..40439)
                     /locus_tag="ACG8H7_000461"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP8982590.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(40534..40971)
                     /locus_tag="ACG8H7_000462"
     CDS             complement(40534..40971)
                     /locus_tag="ACG8H7_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982591.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        11723 a         8627 c         6978 g        13667 t
ORIGIN      
        1 gttatcatta gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa
       61 tttaaaagaa agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga
      121 catatcacca tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc
      181 gaagcgattg ggatcgccgg cccttctgta ttgattgctt ttttgattgg tgggctgatt
      241 ttatatatcg tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac
      301 ggattatctg gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg
      361 gtctactggt ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt
      421 ttgcagttgt ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta
      481 actacgatca tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct
      541 tttgctaaaa tcagtgtcat tatcttactg atcatttttg ctacttatct tgtcggtcag
      601 caaatgttgg gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt
      661 actcctcatg gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc
      721 ggttctgaac tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct
      781 aaagcaatca aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg
      841 cttttgattc tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg
      901 gtatttgaaa aaatgaatat cccatttgca gcggatatcg tcaattttgt gattgtcctt
      961 gctttgttct catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt
     1021 ttgaaggatc gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg
     1081 caacgtgctg ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt
     1141 gtcggtgaca aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc
     1201 tggatcatta tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat
     1261 aaaggaatca gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc
     1321 acgaactcca ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt
     1381 tatcaaaaaa agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta
     1441 ttttatttat gtggaataga tggagctatt gaagaaagga gttgtgacaa aagtcttgtt
     1501 gcaactcctt tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc
     1561 ctctgtgcaa aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa
     1621 aaaaaggagt ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc
     1681 aacggtagtt atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt
     1741 cagtcgtaca cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct
     1801 gttgttcatg tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag
     1861 tcttcttgtt tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac
     1921 agcttggatg aaggccatga ttcctcatca ttctatcgct atcctggcaa gtaaacgagc
     1981 tgatttaaaa gatcctgaag taaaaaaatt agcggaagaa atcattcagg cacaagaaga
     2041 agagatcgac aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat
     2101 aaaaaaagag gatgttccag aagttatcag cttcaagaaa taagaagaag ctacttctgg
     2161 aacgccgtga ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2221 acaaaaatag cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     2281 tgaacaccgt ttattaagag gttgtaaaat cagcgttctg acctgagtat tagagagaca
     2341 aatggaaaaa tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga
     2401 ttgaacaccg tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa
     2461 ttttgtgaaa tggctttcca catttttgta taaatttggc ttaataccga gaaactggct
     2521 tttgaatacc gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt
     2581 atgtttttat tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt
     2641 ttttgagatt ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact
     2701 gaaaaatacg aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact
     2761 tccggcaatt ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct
     2821 acctaataca gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg
     2881 cagaataaca gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa
     2941 tgttgtaaaa atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt
     3001 tgagatcaag ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga
     3061 cacagtaggc atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt
     3121 tccagtattt gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg
     3181 tgggttttga ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat
     3241 tgccggcacc tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat
     3301 tgaaatagta aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc
     3361 ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac
     3421 agttgaccac atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc
     3481 tagatttgtt gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat
     3541 agctccaaca accagcgctg gatttaagcc tagtgttgtc ccaataccga aaaatgcaat
     3601 cccaatcgtt gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac
     3661 aacaaataca gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag
     3721 agttggaatg atccctgctt gtatccatac agcaatcaat gaaccgacta aaataaaaat
     3781 aaagattggg atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt
     3841 cgggtatctt tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat
     3901 attaggtgag acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa
     3961 tacaagtgtt gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt
     4021 tttagttgtt cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc
     4081 cgcgattcct cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg
     4141 cggtaccacc ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta
     4201 tggactttcc atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc
     4261 tgaacaaaac acctaaccgc tactgcgtga aaagttgact tgtacgaagt ttaccttcca
     4321 taagatcatt tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat
     4381 aactttcaca cacaagttta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc
     4441 tactatttct tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc
     4501 aagagctgta ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact
     4561 tttagcagca gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac
     4621 cgtaggctgg attgttggta tgacttggct gacatttcct acatcagaag aaccgagact
     4681 tgttctttgt tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa
     4741 gaataagtca tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc
     4801 aaactcaaaa gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc
     4861 atatacttta tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta
     4921 atctggaatg acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc
     4981 ttttggcaat tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa
     5041 agcatttact cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat
     5101 gtcgactgga tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac
     5161 gcataacgct acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga
     5221 tccgttctcg cccccttctt cccctggggt gccatataca cggatttctc caccagtttc
     5281 atcaatgaat tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg
     5341 cccgcatgca tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg
     5401 accgggttta ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt
     5461 ttctacccaa aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc
     5521 atagttactt acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa
     5581 acgatattgg attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc
     5641 ctcctatgct agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga
     5701 tccttttgat gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat
     5761 tttttgatac gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg
     5821 acgtgcatgg tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc
     5881 atctacagct acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt
     5941 ttgggaagca tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac
     6001 agtgggcgct tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag
     6061 aagagctctg ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat
     6121 atcagctaca tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc
     6181 gatcgatact aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg
     6241 gaaagaattc aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt
     6301 aaatttcacg tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca
     6361 tacatctgta tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt
     6421 atctgctttt ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac
     6481 aaaacgaata atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca
     6541 gttgattact gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa
     6601 tcgttacatc tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc
     6661 ctgcacctac tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg
     6721 agacccgaat gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg
     6781 gactggttcc catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt
     6841 tctcgatttg acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt
     6901 tcgcaccaat cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga
     6961 taatgaaagg gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac
     7021 ggttagctag taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa
     7081 tcatcccgcc aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga
     7141 caaattcatc cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt
     7201 tatcttcctc cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt
     7261 atttttacac cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca
     7321 gctaaagtaa cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat
     7381 aaaatattcg ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc
     7441 tgatttccaa tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca
     7501 gaatgggcaa tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct
     7561 ttggtcaatt cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt
     7621 cttccatctt ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga
     7681 gtgatcagga cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc
     7741 tgaagggtcg ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt
     7801 tcattcgcca aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta
     7861 ggataagcag atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga
     7921 cgacgttcct gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata
     7981 gttcggcttt ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt
     8041 cgcaattctt tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct
     8101 gttggtttct gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa
     8161 ccgacgattc cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt
     8221 atctcttttt ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt
     8281 cctccttatc taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct
     8341 tttttgcgtg ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac
     8401 gatcatactc tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat
     8461 gaattgctcg atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct
     8521 ctctgtcaaa ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa
     8581 agtcctttta ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt
     8641 tttcggaagt ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt
     8701 aacggtcgaa cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt
     8761 cagcttttct tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt
     8821 actttccttt tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact
     8881 gaaaagtcaa gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt
     8941 taacatttca ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt
     9001 ttcttcgacg gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc
     9061 tgctcgtgaa gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc
     9121 ttctaatcct gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga
     9181 cacttcgtct ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat
     9241 atctgttgct cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt
     9301 taagtctgcc aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg
     9361 ttgataaaaa tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc
     9421 tgctttcact aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat
     9481 ttcttttggg atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt
     9541 tttttctgtg tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt
     9601 aatcctttcc aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa
     9661 agaaccagcc ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca
     9721 gctgtcacaa gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca
     9781 ggaagtggca tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca
     9841 ataattccta atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt
     9901 actttcggtg tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac
     9961 catcctgcat ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa
    10021 cttccgccca ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca
    10081 aatatcgaac ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat
    10141 ttttttggct cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa
    10201 acatcataga tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg
    10261 aacgtacttg caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg
    10321 atatatttca tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc
    10381 gtcaaatctt tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta
    10441 tctacagaaa taccgatatc agccactttc attgcaggag cgtcattgat accatctcct
    10501 aaaaatccga cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga
    10561 ggattcaatt ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc
    10621 atttccgaga ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac
    10681 actgaacgtg taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct
    10741 ttcagagctt tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa
    10801 ccaatcaaaa ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg
    10861 gtcttttgtg cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca
    10921 agtacggctt cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat
    10981 ttagaaatgt tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct
    11041 ggatcagcaa ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact
    11101 ttttgataat tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca
    11161 tctaacaaat ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca
    11221 cgatcatctt catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc
    11281 aatgtccccg ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc
    11341 aaatttttga tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc
    11401 gtaacgatca ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga
    11461 aaagcttcta gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc
    11521 aatgacataa atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat
    11581 tgaattggtt tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca
    11641 gaaacaatga ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt
    11701 tcgtaacttg tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga
    11761 tagctttccc ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc
    11821 atatccgctg gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct
    11881 tccataggaa tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt
    11941 gatttcagtt tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg
    12001 ctgacgataa ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct
    12061 gctaatacat attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat
    12121 ggcgtgatgt atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc
    12181 gcattttcac cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct
    12241 gatgcatgga atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt
    12301 ttttgtgtat ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt
    12361 tctcgctgct tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc
    12421 tgagaacaga tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg
    12481 tcatccattt gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt
    12541 ttcgaatagt tatttttcca attagaccac ctccttttta tttttcaccg atggggattt
    12601 gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa
    12661 aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc
    12721 tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt
    12781 ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta
    12841 ttcggttttc cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg
    12901 tttttcatac tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt
    12961 ctcattttcc acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa
    13021 agaacacttg ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc
    13081 caacgttttg aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct
    13141 gaattagcaa aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc
    13201 gattacatga cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat
    13261 atcgtccgta agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga
    13321 ggagactacg cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct
    13381 gcggtaaaag agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa
    13441 gcggcgaagt gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag
    13501 cagttttttc tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa
    13561 gaattcttgg tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata
    13621 ataatctgcc atcaatgttt catagatcac tgtacttctt tctttatgtg ctaagctccg
    13681 tttcatatct tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga
    13741 agtcaggttc attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg
    13801 tcgcattctt tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca
    13861 aggaaaatca gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta
    13921 ttcttcaatg attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt
    13981 tttttcgacc atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg
    14041 cctacggtta ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag
    14101 cattcgtctt tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata
    14161 ttctgcaagt gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt
    14221 tttcactgaa tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa
    14281 ttgggactgt gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat
    14341 aatagttctc ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg
    14401 aaaaatacac gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca
    14461 ttagaatgtt gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg
    14521 ttcaacgtct gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga
    14581 aaagacactt gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc
    14641 atttttatag tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg
    14701 caagagattt tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg
    14761 atctttggag atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg
    14821 attattgtaa gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg
    14881 aaaagcaact acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc
    14941 tttatcatcg attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta
    15001 ttcatgattg actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg
    15061 catctgtgcg ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa
    15121 atctttatta tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt
    15181 ttggtaaata ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata
    15241 atgtgccgtt cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc
    15301 gaaattcttg tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta
    15361 aaacggctgt tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa
    15421 tatcccataa cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc
    15481 ataacctaag tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa
    15541 aacagcgctt gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt
    15601 gtctgaccca agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa
    15661 catattttca gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct
    15721 gatcgttgct tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac
    15781 cttcatatta atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc
    15841 tttagcgatc ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa
    15901 aacttcatca atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt
    15961 gattccgtca taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc
    16021 aaatgttcgc tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa
    16081 aataagccct gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt
    16141 gataggtatt ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag
    16201 ccagtagatg acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga
    16261 cactttcgaa aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa
    16321 aataatattc aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa
    16381 cgctggcttt ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat
    16441 tgggttgatc cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc
    16501 atagtaggca atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa
    16561 acgattttgg cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt
    16621 tttcttattt tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt
    16681 tttattcttt ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa
    16741 gcgattaaac taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca
    16801 aaagccagtt tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc
    16861 tccttatttc ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct
    16921 gccaactcct cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat
    16981 tgttccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa
    17041 tcagctgacc ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac
    17101 ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct
    17161 attttttgaa attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata
    17221 cacggtgttc aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
    17281 tattttgccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctgttt
    17341 ttcattgtcc ataggattga tattgtcctt gtgatgactc attcgtaata attgaggctt
    17401 gattttcttc acttcccgtt atcgtttgtc cactgccaga atctgaagga cttgttcctg
    17461 tcgactcgtt tttttgagaa gagacagttg ttttcttgac ttgttcattt gtctttttcc
    17521 attccgctaa acgatcggcg accacttttt tgtctacttt ttcagaataa gcggtgatca
    17581 cttttccatt tttcaaacgg gctactttgt cgccatcatg agtcaaaagc aaaacggtca
    17641 aatgcttgtc tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat
    17701 taataaattg attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt
    17761 aattggcagt tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat
    17821 ctactgattc tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca
    17881 tttttcctga aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga
    17941 gaagaaacgg tctttttttc ctcatgcttt cctccactcc tatttaattt tgactattct
    18001 attatataca aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt aaataattga
    18061 acttcttttt cacaaaatgg taagaatgga gaatttcacg catttgctct caactattct
    18121 ttcttaagcc tcaaatccgt ttttatctga tacgtctttg aaccaatgac ccgatttttt
    18181 gattgttctt tttccatctt gtgctaagtc tactgaaata aagccatatc gattcttata
    18241 tgcattggtc cacgaccagt tatccataca tgtccacata tgatagccta aacaattggc
    18301 accttcttga atggcttgat ggacatattt cagatgagat tggacaaatt cgatccgata
    18361 atcatcgtgg atcatgccat cttctttgat aaatcgtgct tcgttttcta cacccatacc
    18421 gttttcagaa ataaagcagt cgatatttcc ataatgatct cgtacattga tcaaaatatc
    18481 atagattcct tgttcataga tctcccatcc gcgataagga ttcattttac gaccaggcat
    18541 ttcataattg tcgaaataat cctcaggcat tggaccgttt ttagtatcga ttggtgtttc
    18601 ttttgctttt acccggcgtg gctggtaata attgatacct aaaagatcta ctgtattttc
    18661 tttgataacc gtcaaatcct ctgcatggat cacaggcatg agatcaagtt cttttactat
    18721 ctcaatcaat tcacttggaa actcgccttt tacagctgga tcaaggaatg aacgattgaa
    18781 gaacgcatct gctatttgcg ctgctttaac atcttcaggg tttgattcgt ctcttggata
    18841 gcttggtgtc agattcaaga tgataccaat aacaccacct tgctccattt catggtaaga
    18901 tttgatggct aacgctgaag ccaacgcttc atgatagcca acttggatcg cttttttcat
    18961 atctgactct tctggataat gccatcgata taaataacct gcttcgactg gaacgattgg
    19021 ttcattatga gtaaaccatt tttttacttt cgaaccaaac aattcaaaac aacttttggc
    19081 atatttttca taggcttcaa ccgtttctcg gttcaaccat ccgccttttt cttgcagata
    19141 catcggcata tcgaaatgat acagattcac aaagggttcg ataccgtttt caatcaactc
    19201 atcaaaataa cgatgataga aacgaacggc ttcttcgttg attgttccag attcatcagg
    19261 tatcaaacgg ctccattgga tagatgttcg aaatgaatta tgcccagtct gtttcatcaa
    19321 ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc
    19381 aaagaacttt tgcggttctt ttttgtacca ataatcaaaa atgttctctc cttttccatc
    19441 gcctgaaatc cttccttctg tttgtgggcc acttgctgca gaaccccacc aaaaatttgc
    19501 tggaaattga tattttttca tcaaagcacc tctttttctt ttttagttcg ttttagttga
    19561 ttccatgtat gatattttcc atcacaatca ctttttcatt ttcttcctct tcttttttga
    19621 ttcgttttaa cagctgttct gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg
    19681 tgattttcgg aaggatactc agatcgtttt tatctaaata atcatagtga ctgaacgcaa
    19741 tatccttccc gattctgaca ccttcttgat tgaaacattc gatggcacca atcgacatat
    19801 tgtaattgct gacaaaaata gcagttgccc ctttttctaa taatgctttt gccccgtaat
    19861 agccgctttt tctagaatag tctcctgtaa agacctctaa ttggatcgat ggatcatttt
    19921 ggaccgcttc tttggctcct gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag
    19981 caataaatcc gattttttta tgtccttgtt caccatgatg agcgatcact tttgctacac
    20041 tttctcgatc attcactaat attgtatcaa tacctgctgg tccttctgga caattcaatg
    20101 cgataactgg aatactcatt tttgtgagca tttcaaatcc agaccactct tcttcggata
    20161 agaagacaat cagcccatcg atcacatgct tcatcaggta ttcaattttt tgttcaattt
    20221 tttctggctg atcggcaaac ccgcttaata ataagctata cccattttct tcagctactc
    20281 tttcgatccc tgagacaact tccgacccaa aacggctgga aatcccgttt atcaaaagtc
    20341 caatagagaa gcttcgatta ttcttcaacc ctttagcaat gatgttttct ttatagtcca
    20401 acgctgaaat cgcttctgct atttttttca tgttcttttc cttcagttgc tgaccattca
    20461 agtaacggga aactgttccc actgaaacac cagccatctt agccacatca cggatcgatg
    20521 ccatacattc tcactcctgc cagttacatt tgatttctct ttcttgttgt tcgattatga
    20581 tgaccgcatt ctttgcatag tgttgcttgt catcaatatc catataacta aagtcttcag
    20641 tagagcaact tcctctcact attaattata cttaaacaag tatttgaacc ggttcatttt
    20701 taatatagca tttttttaaa agattgcaac acttttttaa aacgctttct gaaagtggcg
    20761 gattttttgc ctttttaaaa ttttattcta aaatttactc tatttgtggg tgttttgtgc
    20821 gtttttctct tccatccgtt tgaagtttta cattccaatg gtataatttc tttatcctat
    20881 caaatggggg aagtattttg aagaaattaa tcgtactatt tattttgtct gcgggacttt
    20941 tgacattgac tggctgtgat ttgtttggcg gctctgacca atcctcggac gaaacaaccg
    21001 attcttctgt cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat acgctaaaag
    21061 tacgtaaaga tatggattta tccgcgggac agttgacatt tacagataaa aaaatggaat
    21121 ggacacgcac gtatcagaca caaggaactg attcttctga atcatctgaa gaaaaaacag
    21181 tgctgacaga tatcaagatc actaccaaag atgacgaata tattatttca ggaaaagaaa
    21241 ataaaaaaga aacaacagtc atatttacaa aaatcggtaa ttaccgtttg aaggatgagg
    21301 atggcaacat ctacagtcta tagtgccgtc tgttggatct tcttttgttt ctccccttct
    21361 tttctgctat agtaaagaag caagatgatt gtaaaggaga cgaaatggat gatcgatagt
    21421 cctgattatt gtacatgtaa taacaacaaa aatatccata cagaatttaa tgaccaagga
    21481 agcttgagca tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa
    21541 tcttatcctg ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact
    21601 cactatggct gatttttatg aacttcaaac tcagcttctt cctttgatca agcttcatca
    21661 gtaaaacaaa cagtggatca tttcagttta caaatcaaag agtaaagatt tatttatttc
    21721 aaatatttga atataaaata accaaacgat agaaaggctt attctacatc acgtagtaat
    21781 ttttctttcc cgtcgtttgg ttttttattt ttagcctaat tcttgacctt ttgcatgctg
    21841 gttatccgta aaatattttc cagcaggctt tccttccaat tcagctaatt tttcttggat
    21901 ctgacggatc accttttttg catcttctgg attttctaca aaatcgaatt tatccccatc
    21961 cacttgtatt ttaggacata catcaaaatc ttcataccat tgattatagc gggtgttcaa
    22021 cgtttcataa taggtatata acgtctcatc gctttctagc tgttcatagt cacgcccacg
    22081 ttttttgata cgttctagca tcgtgtccaa agaaactttg atatggacca tcaaatcagg
    22141 ccgttttttt ggagcaacat catctaactc gtttagcatc gtatctaata agctatcgta
    22201 ctgctgtact tcgatatccg ttacccgacc taaatcagcg tttagatgga acaacaggct
    22261 atcttcatag atggatcggt ctagcacatt gtcatcgttc actaaagcat ttttcattgc
    22321 taaaaatcgc ttgttcaaaa agaagatttg caataaaaaa gcgtactgct caggatttga
    22381 ataaaagagc ggcaatactt cgttatcttc gacattttca taaaatggtt tagaatcaat
    22441 ctcttttgca agcatatccg tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa
    22501 aatcactgac atacaaccct tcctcttcta taagtactcg cctacgtaag tttcaacagg
    22561 caatgtttca aaatcatatt cacgtacagc ttggtctgat tttaaatacg tatggagact
    22621 ttgtgtaatc atcaagttcg taggtaccgt ctcttcttgt acaatgatca tcggtttttt
    22681 caacatagtt gccacaccta attcgtaagc cgttcctgga tcgattgttt gatgctcaaa
    22741 gtcaaggata gcaacaacaa attctgcatt tgtcagttct tccatatctt tttcgtatac
    22801 ttcctgcgcc catccagcgc tgaatagttc ataattgctt tcttggtgat gacgtggaga
    22861 gtagaaactt gtgactgttt tgttttcctc caacgctttt tcgatacgac ttactcgatc
    22921 gatttgctcc tctgaaaaaa atggtcctgc taaatatatt tgactcatta ttttgcctcc
    22981 ttcaataata actgattttt aatagatact gctcgtaatt cattgaccag ttccaatgga
    23041 ttgttggaaa agattacttg atccaaccat tttttatcca taaaatcctc ttttgcaaaa
    23101 gtttctaata attgttgcaa gccgctgtaa taaccgccaa tattcaatat tccgattggc
    23161 ttgtcgggcg tcagaccgat tgctatccaa gaaagaagtt gtgagaattc ctccaatgta
    23221 cctagacctc cagggaaaac gaggacagca tctccctttt caaataataa tacctttctt
    23281 tcatcgatcg tttctgtagg aataaatgta gtaacatctt ttccaggcaa ttcagtttca
    23341 aaaagcccat tcggatagat acctgtgact tttccgttat tttccagtac tgttttacta
    23401 atcactccca taatcccgga gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc
    23461 atgtattttc ctaacgtttg cgcaataaat ttgtaacttt ctttattacc aaaacgggaa
    23521 ccgcaaaaaa cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt
    23581 ctacacgtgt ttttttaaaa taaaagacta gattttgtag ttttttagtg gtatatagga
    23641 tagacgccac tatatatagt atttagaaaa aaatcatttt tcagccattt tatagataaa
    23701 acaaaaataa ataacgtgtt ttcatcgata tgaaatgact tacttataga agatactggg
    23761 atttcgtcct atatgccaat aacgtgtatt attttaaaca acaaatactt gtttctttta
    23821 ttaacgaaaa aatcattttg ttctcgtgtt atgtttcact gataaatgat gtttctctaa
    23881 ataaaaaagc cgacgaacta aacgatcaaa atttgatcat ttttgttcat cagcttctcc
    23941 taaaaatctt tttgttaacg ataaatagct ggatttgtaa aaatatcact tgaatcgtca
    24001 ctgctggaag aaaattcagt gataaccgca cctgttgcgc ctgcctggaa ctagtgtttg
    24061 atattgggct cgaacgtgta ctgatctcta gcatgaagaa taatttcttt ttttactgta
    24121 taataacttt ctttcctttt tggaatagta caggaaagtt tttttgtttc ctttccttcg
    24181 acataaggta taccagaccc tttcgacaac gaaaggttct catttttcct tcatcgtcat
    24241 ttcaaggagg gtgccgatgc tctggacagt ttcgatcgct cctaaaccaa aatctgtata
    24301 ttcgattctt tctaactctg attctttcat accattcctg tctcaaggta catctattgt
    24361 acttttttct tgtaatccat tctgcctctc ctaactaatt gtcaaatcat cccaagatat
    24421 gtcttcttcc tctttttctt cttggatgat ttctgcattc ggatctactg gttttttcaa
    24481 taataaaatg gttattcctg tgacaaaaga accagctaac aatgcaccaa tgaatgccca
    24541 tggtctggtc atcgtagcga ctgcaaacat tcccgatgcc ggaatacggc tatctgcgcc
    24601 ccaaaacatg cttaatccgc cacctactgt cccaccgact cccgttgcta tgacattgcg
    24661 tgccaagtcg ttcaatacga tagggaaaca tccctctgtg atctgacaga tccccatagg
    24721 aaaagcggtc ttcaatgtat cgatttcttg acgattgagt atatttttac gaatgatttt
    24781 ccctaagaaa taagcgatcg tcaagccgaa tggggttacc atcgaagcta aaatcaatac
    24841 agtgattggt tcatagattc cttctgaaaa tcgtgccaca cccccttatc cagaataaat
    24901 ggtaatccag aaacagccgt gtatctgcgg taataagcct aaccaaacga aaaatatgag
    24961 aagctatttt tgtttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
    25021 ttttttattt atgtttcata atcaatatgg taaggatgca gctccgtatg gtagaccgcc
    25081 tccgaatatt caatcatttc cccaatttgc gtagacgatt tccgtatccg tttgatgccg
    25141 atctgtgcgt ctgttttcag aattccttgt tcttcttctg ttaaaaatac tgccgagaaa
    25201 tcatcagaaa atgagtcaat agtatatcct tttttcgtca gcaaacgata caaagattct
    25261 ttgccaaatt cttctctgct gaattgcatc atttcttgtg gaagataatg aacaaaataa
    25321 atataaggct gattgttcaa ataatataag cgcttaaaac aaacagcttg ctcaccgaaa
    25381 aaggaaaaca aaggatcttc cttctttata tggaccaatt gcaaatccag cgtttctttc
    25441 cggacttcat atcgggagtt ctccaagatt tgcgtaaagc ttgccgcttt ggataatcga
    25501 ttgtaaggac gattgcttag aaccgtcgtc ccacgaccac ttttctttgc gacatattca
    25561 tctgatgcca gcagttcgat tgcctttcgg acagtgattt tgctgacttg aaacagttct
    25621 tctaattcac tttctgtcgg caacatcgaa ccgactggat attttcctga gaaaatatct
    25681 tctttaattt tatctgcgac ttccaagtat agtacacgtg tccttttcat aacgtcaccc
    25741 tttgctttac tattactaaa agaatagcac ataaaaaata aaaaaagaac acaaatcaca
    25801 tgtgagaagt gtgttctttt tcattcagct gctttaatta caagtttacg ccatttgagg
    25861 caattacttc tttataccaa tcaaaagatt ttttctttga tcgattcagt gtcccatttc
    25921 cttcatcgtc tagatccaca tagataaagc cgtaacgttt actcatttct cctgttgatg
    25981 cactgacaag atcgatacat ccccaaggcg tatagcccat caaatctaca ccgtctgcta
    26041 ctgcattagc catcgcttcg atatgacgac gtaaataatc gatccgataa tcatcaataa
    26101 tcgtgccatc ctcttccata tgatcaatgg ctccaagacc gttttcaaca atgaataatg
    26161 gtttctggta acgattgtat aattcattca atgcgattct cagcccttct ggatcgatct
    26221 gccagcccca ttcacttgcc tcaagaaacg gattttttac tcctcccagc aagttccctt
    26281 ctgaagttgc tgcttgtgga tcagtctcat tgattgccgt agacatataa taactaaatc
    26341 cgatgtagtc cactggatat tcttttagca actggaagtc tttttccgtt tgctctaatt
    26401 tttccggatc gactccatat tttttatggg tacgcgctgt gtatgcaggg tattctccac
    26461 gtacttggac atccgtgcag aaaaaattga attcttggtt ttggatcatt gttgctgcct
    26521 gattgactgg attggcatcg atgctgtaag ttgttgcata gataatcatg cagccgattt
    26581 ggatatccga acgcaattcg tggccgattt ttacggcttt gctacttgca acgaattgat
    26641 tatgccatgc ttggaaaata ttttggtact ctcctgctcc attgcttttt actaatcctt
    26701 gacttaaagc tggaaagtga aaggccgaat tgatttcatt gaaagtcatc caatatttga
    26761 ctttactgct gtatctctct aatactgtct gagcaaatcg ttcatagaag tcgatcagtt
    26821 ttcgattttt ccagccaccg tattcttttg ccaaatgaag aggcatttcg taatgagaaa
    26881 tcgtgatcac aggttcaata tcatatttta agcactcatc aatcagctga tcataaaatt
    26941 ctaatcccgc ttcgttagga gtaccttcgt ctccatttgg aaaaattcgt gtccaggcaa
    27001 tcgaaaaacg ataacatttg aatcccattt ccgcaaacaa ggcaagatct tctttgaaac
    27061 gatcgtaatg atcgattcct cgatgattcg ggtaaatata tttttcttga tcaatcgtcc
    27121 agtcaaattc ttcactgccg ataatcgcaa agcgctgctt acctccaggc atagcatctg
    27181 caacggatag tccttttccg tctcgatcat aagccccttc taattgattt ggcagcagtt
    27241 gctccacccc ataaaaattt ttctgaaaaa cgtgattgca tcctgatttc ctccttattt
    27301 tacaattgcc agaacatctt cgtttgcaat aatctctttt tggtctaatt caagtatatc
    27361 ggcaaatgca tcagtattcg taatgacaac tggagtgatt acgctatagc cagctgctct
    27421 aattgcttcg atgtcaaaac gaatcaataa atctcctgct ttgacgagat ccccttgttt
    27481 gactaagatc tcaaagccat tcccatttaa ttctactgta tccatcccga tatgaatcaa
    27541 aatctccgct ccgtcagtcg ttgtgatccc tacagcatgt ccagttggaa aaatagtcgt
    27601 gatctctccg tctgcaggtg caactagttc tcctacttct ggatcaatag cgataccttt
    27661 tcccatcacg ccagaagaaa aaacttggtc tggtacttca ctcaatggca gtactttacc
    27721 cgttaaagga ctttttaagg tatgccgtgc tgaagtaatc ggttttcctg cattcgcatg
    27781 tttgttttct aattgattgt cttgtgtttg ttcgtcaaat cctaaaatca atgtacctac
    27841 gaatgcaaga acaaaagcaa ttcctgtagc tattactgct actgtcacat ttgactctac
    27901 accatccacc gtattaatga aagtcggaat cgtcaataag ctgaccaaac tgctggagaa
    27961 agcttttact ccggaaaatc cgataatagc tccaccaata cctccagaaa tacaagcggc
    28021 aatgaatggt ttcttcaaag gtaatgtcac accatacaca gttggttcag tgattccaaa
    28081 taacgaagtc actgtagaag aaatccctaa tgctcgaagt ttcgtatctt ttgttcgcaa
    28141 tgctacagct aaagcagccc caccttgtgc caagatggct ggaagaagca ttggcatcaa
    28201 tacatcaaac ccatattgtt cgatattcaa aaacatgatc ggtacgaatc cccaatgcat
    28261 gccgaacatc acgaatactt gccataaact tcccatgatt agacctgcta cgattggact
    28321 aaatccataa atcgagttga agagaccgcc taaagcatta ccggcaaccg tcccgattgg
    28381 tcctaaagca agaaatgtta atggaaccat cactactaaa acgacaagcg gaactaaaat
    28441 catttgcaga aattgcggaa tcactttttt tacgaatggc tctaatttac tttgaaccca
    28501 tacggctaga atgattggta tgacagaaga agtataacca gatggtgaaa gaatcactgg
    28561 gatcccaaag aaactgatag cttctcccgc accagcaact gctgtgatag ctggatacac
    28621 aagtgccata gcaattgcta ctgctaaaaa ctggttggct ttgaactttt tagctgctgt
    28681 aaaagcaagc atgacaggta agaaggtaaa gacaccatct gctgcagcaa acaatatttg
    28741 ataagtaccg ctatcagctg tcagccagcc catcaccgtt gccaatgata agaatccttt
    28801 caataccccg gcggctgcca tcgcccctag aaaaggtgta aatatactag agataatatc
    28861 gatcagacga ttcaaaatat ttttggactc ttcttttcca tcctccgaaa ctgcaagacc
    28921 ttcctcatcc acgatcgatt gataaacatc tgctacatta ctgccaatca ctacttgata
    28981 ctgaccgcca ctttgtacga cttggatcac gtccgggtct tcttttaact tttgcgtatc
    29041 tgctaaagat tcatctttta atctgaatcg caaccgcgta gcacaatgta ctaaactttt
    29101 gacattcttt tgtcctccta ccgcttccca tacacgacga ccaactgctt gattatccat
    29161 tgttttttcc ttctttcttt tttatttaaa aaataaaaac ccagattgaa aaaaacagtc
    29221 acaaacattc gtgaacctat tttttcaatc caggttttgc ctgccataca gtaacaatcc
    29281 ttatgattgg atgctttttt tctcacttac tactctttga atatgaatcg ttaaataaat
    29341 ctgctctgcg atccccattt cctgtttttg cgtttgtcga agatactcat tgatccgttc
    29401 gacacaatga aacgcttttg gatactttcc ttgaactaat gcgtagagaa attcatcttg
    29461 ctcgtcagat ccttcccctt tgagataacg ctgggcaaaa aactgcagat gagtaatgat
    29521 acgctgataa ttcaaagatt cttcatcgaa cacgatgccg aagaatttac tgatgatact
    29581 cagtatatct cttaccattg tggtagcggt catcgtcact tgcatgtctt cgtttccttg
    29641 ttggctattc acaaaatgaa aggcaataaa accggcttca tcctcagaaa gttccacacc
    29701 cgtcttttgt ttcacgaaca aaagagcctc tttcgctgct tgatattctt tcggataaaa
    29761 tttttttgtt tcaaataata atggattagg cagttggata ttttcttttg ctcgtgtgat
    29821 cgcataatga agatgatcgg tcaatgtcag ataaatatta ggcgaaaatt caacttttag
    29881 tttatcttca gccagttcga ctacttgttt gacccattcg atctcctctg ccggaatatc
    29941 agccatcagc tgctggaatt ggtttttagc aaaagaatct cttaaaacaa attctttttg
    30001 gaccgcttct ggcgaaatct cgtccccaat ctttttttga aaagctagtc cttttcccat
    30061 acaaacgact tcttccccga atccattttt cgaaatgacg acattgttat tcaaaatttt
    30121 atcaattttc attttgcttc gccctctcaa gaaaaataaa aaaacctaga attcgcctac
    30181 tgtttcttag ctgaattctg gttttgcctg ccgtacagta acaatccatt tcttattcaa
    30241 aatcatcata ccatcgaatt tttatttatg caagcctttt catataaaaa ataaaaaaac
    30301 tggaacacaa gtctatctag aaaacaacca aatcagagat gcacctcttt gcgtttctct
    30361 ttttttcagc aaattggttt ttccttatcg attcatatcc tttggctgtt tttaatctgt
    30421 aacgacttat gtcccagtat cctaaaatgt ttttgtctct agattgaaag cagatctcat
    30481 atcatctgtc gcatctttta tcgatgtaac actttccagt tctccttgta caactagtcg
    30541 cgtgacggcg tccatgtcgc cacaagtaat tagtgtgaca atttcttttc cctcgacttc
    30601 attcagtaat tgggtatctg taggagcaac tttttcttta aaaaatgttt tgtatgtata
    30661 cacattttct aaatctgtca aatagatctt ggcacccatc tctaaatttt ctagcggtgt
    30721 aaacagcaat tcagggttcg ttgcacgatg gcttgctaag gcataatttc cttttcccat
    30781 ttcttgatct ggagataatg ttcctgctcc gtaaagtagt gacgtattat ccaagccttt
    30841 gaaaaccggt aaattgattg aaactgaagg gactgcgata ctagcaatca ctggtagatc
    30901 tgttcctctc atttgcgacc tcatgacccc ttctgtcgtc attggttcta ccgcatcaaa
    30961 gtcaaaactg acatctttat cgttattctt tttcagatct tcttttgtta cattcgctat
    31021 agcatattta tctcctgtct ctctgacaaa ataatccttg atctgttcat tgaaaatcaa
    31081 agctagtcct actaataaca agaggaacaa gaggatattg actagccatc tttttgcttt
    31141 tccttttttt ttcattttct ctccctccag actcatttta ctgtttttct tttgctcttt
    31201 ccaacagccg aatgacacat ttctacaact ctgtgtattt ttttcgtaca cctgcgcttt
    31261 tttctacagg atatttcaaa ttatttttga tcaatttttc ttcaatgatc tcatctagat
    31321 ccaggtgaag gttgtctgcc atcatgtatg aatagatcaa tacatctgcc agttcttctt
    31381 tgattcgttc taattgtgtt tcttttactt cttccggcgt tttccattga aaaagttcta
    31441 acaattcaga tgcttccaaa gaaatagaga tagccaaatc tttttcgtta tgaaattgtc
    31501 gccaattacg ttcatctcta aactggttga ctttttccat actattcatt tgcttgcctt
    31561 cttccaatca cttcttactt gattaatcat accatttttt tacataaaaa atagatgatg
    31621 cttttaaaaa aactcctgtt ttcttttatt taaaataaat gtcatagaaa atcaataatt
    31681 taggtagcca ccatgtgtat gcagtagcgt gtcttcttcc cttgaggctt tttgtttttc
    31741 gatacgttct tcttcccaaa cagacaacgc tactagaatc tctcttaatg attttccttc
    31801 ttcggtgagc gagtactcta ctttaggcgg gacttggtta taggcttttc gatggatact
    31861 ttcatcttgt tctaactctc ttaactgctg ggtcagcatt ttttttgtga tcgtagggat
    31921 ttgcttgctt ctccacgcct aaaataagcg atattgctct tccctttatc tggtgtaagc
    31981 aaagcagtta gcattttgac gatcgtgggt tccgcttttc acttcttatc tacttagtat
    32041 catgaagtat actttcgtac taatactaag tagcggaagt gtcaactact ttgtttaact
    32101 acatagcaaa agaaacagaa gggtgacaac ggtcatcacg cttctgtttc ttttgaatat
    32161 gctgtgttgt tgtctctatc tccgtgtaga ctttcctcaa acttatcctt tgacagtaag
    32221 tcagtaggct gatttgctct ttctagatgc cagatacatt tctacaaaaa aatagtgatt
    32281 ctgttttgaa aattcgattt ttagttctgc attttcgtaa acatccaaca tttttttcgc
    32341 actgtataga cctaacccgc catttttttt agaggttttt cctcttttga ccatctccga
    32401 tagcgtcaaa ttcgttggat ttgccttgtt cgctatacta aaacgtgatc cctctttttc
    32461 ttccagcaga ttgatcgtaa tcggactctg atcggctaca cgatgatcga atgcgttaga
    32521 aataaaaata gataaacagc gaataaaaac aaccagatca atcgggattg ttttcacttc
    32581 gacaggaata actacttgga tcgggatttt ctccgattct gctttttcta gaaattttaa
    32641 aaagacaccc ctgacagctg gggatacaat atttgttagc tgttgtattt gcggtttttc
    32701 aaaaatagct gcagaatgat cttctaatat ttctaaaaat tccaacgttt cttttttatt
    32761 ttcctgaaga gtaattttta atgaaaacag tagactttta taatcatgtt tgaattcctc
    32821 tagatgatgg taatattctt catttatttg atacagttga agcgattcct gatgttgtct
    32881 ttcttttact tggaaactga tcaacataat ggaaaaaaac aaagaaaaaa aactgatgac
    32941 tatcgacaaa agagatagaa gcagataatt agatgagcgg aagtgaacac ttcccaatac
    33001 caagtaaatc acgccccagc ttaataaaat acaaatacct ccaaaatagt gttcagttgg
    33061 ataagtaaaa agcaagttga gaaatctcgt ctgtttgtct atacttttta ctaatagata
    33121 gaaaataaat gcgataagta gtgcaaaaaa acttgctgtt ccaagatgtc tctttcctaa
    33181 ataatccatc agatcaaaaa ccagaaaaaa gctgagtcca gtgatagcaa aaaaaagatt
    33241 actgatcaaa cagctgataa acaggttttt cgtagtaaaa taaaaaagaa acatcaataa
    33301 taagccaact aggtaaggaa agctcacttt agggatagga agtaaaagtg tcaccaccat
    33361 caaagctata caaccaaagc tgttttttac tagcagttgt ttcgaaaaga ataaatagat
    33421 acctaaatct aaaatcaaag taaaggtcaa ataaagtgtc actgttgcga ccatcttatt
    33481 cctccattgt tttgtttcaa aatgtacaca tattttttta ataggagcct gcatatgaaa
    33541 aagtatttta tcatagaaga taaccagttt cacagcgaat ggatcatcag agaattggtc
    33601 aatcatcata tcgagaatat cgtgcagctc acttctatct ctcaaatcaa tcagttgatc
    33661 cagttgggca ctattgataa tcacgatgtt ttctttatcg atatcgactt gaagcaatca
    33721 ctcacaggta ttgatgtcgc cgaacaaata cgtaaaaaaa atgcacatag taatattgtt
    33781 tttttcaccg cttatacgaa ttttgcagta gagatcatca atcggaaaat ccttccgatt
    33841 ggatatatca gaaaagacag gaatttatct gaacaaattc gtcaagtgct agaacagatc
    33901 aattggaaag aacagcagat cgtaaatgtc gataaaatct ttattgatca aaaaggtgga
    33961 agtctcgtcc tgtatcctcg ggaaatttta tatattgaaa gtatcaaagg tttgaaaaac
    34021 caagttatgg tcaaaacaac tacagaagat aaattagtga atacttcttt aaaacaattg
    34081 aagacagagt tagaaaagta tgcttacttt ttacttttaa aatcttactg tatcaactta
    34141 tcgcaattgc agaaaattga ttctgctcat tatgtgttag aatttttcaa cggtgactcc
    34201 ttactcgttg gtcgaaagat ctttgaaaag acaaaggaac gtttccatga cttccaaaaa
    34261 actgcttcga gttgatgaat aagagctgag acgtaagcat agcagatttc agggagtggg
    34321 ccttgaatcg gctccactcc ctgaaatcgt tgatatgatt ttacgcttcc tcttctgtag
    34381 gagcatctgt taacgaccat gtgaatgttg aaacgtagtt gtctacagca taattggata
    34441 caggaacaaa taattccact gattgtctcc cttgttccac attctctcct aatgaataaa
    34501 tccatgtgcc aatcccttca tttgctcctg cagttactag atctactgcc tgatctgctg
    34561 ggatttcgat tccttcagcg ccagttccag aaacggtaga tggcattcca ctgctattag
    34621 ttgcgctatt tcgaatcacg gcgtttttga atttcacctt tgaaccagtt agtttattcg
    34681 tatcagatgt tgtaccaata aattctgttc gtttgactga taatttccaa ccggcataat
    34741 ttccgctttt atccgttact tgtacgaaat tctgtacttg tgtttgtgct ccagtactgt
    34801 ccgttccgct ttgtaatgca gcatatactg tttttgctgt acctaacttg attgttccaa
    34861 aatcaagatc tggagcttgg tcaatcgtca gtaatccgga tgttccctga tttggttcgt
    34921 caggtgtaaa aggagtatca ggatcatttg gatcaacagg aggaactgga acaacgtctt
    34981 caggatttgc agcatctggt ttggtcacag aaatcgttcc ttctgtagtg gctgagctct
    35041 tattctcagc aaaaacactt tgtgtacata ataatagact tccagctaga acggtactta
    35101 atgcaattgt ttttttcatt tctttttcct tctttctttt aaaataaata atccagcaaa
    35161 taagccaata agaatgactc caacaagtat cagccatcgc cagtcaaatg tttctggctg
    35221 attttttaaa tcaatatcct tttcattcaa ttggtccgct gcatcatcag ttatttcaaa
    35281 atcggtatgg agattccagc gtttttcttt ccaagcaccc ttcacaaccg cttgataatg
    35341 tcccggagct aatttttttc cttctaatgg gacaagtaaa tcaaaatttg tattgggtgc
    35401 tacatctatt ttttcttttt ttattgaaag gtaaggctct tttgtttctt ttttgtagat
    35461 ttcaaccgat gtagtcaagc cgaacatata agaagcattt ggattttgaa gattcgcctt
    35521 aatcacattt cttccatcgc tttgatccgc ataagctttt tttaatttca aatctgggga
    35581 agactctttt tcgctatttc aaatcaagat tgctttcact tgagaaaact gattgacgac
    35641 accaactgta cttttgttag aaacgttctc ttttttttct tgaaacgtga tccctcctgc
    35701 gaccacacca gccagctctt tttctggcgc tttcagtgaa agtgtctcag tgaccgcact
    35761 ttgaggagga acagttattt ctcccactgt ttttgctaag tttttcatat cataacttaa
    35821 tgtttcatca aatttacttt ttgattctcc atattcgatc acaccgtttg aattcgtcgt
    35881 agcggagtgg atcaatgtct caactgttac tgatttatct gttcggttaa agaccgtgta
    35941 ggggatttcc tgtgttttcc caggctccat ccttacattt atatatgttt tgttcttatc
    36001 aatctgatta gccggtaatt caaaatctac cgcaaaattg aattcggagg catggacagg
    36061 tgtctgccaa aggatgaatc caaggagaag gatcataagt aaagagcagt tgtttcgatg
    36121 acttttattc catattcgat tcatcaatca tcctccttga tctttattct gtcggcgcat
    36181 ctgctaatgt ccatgttatc tctgctgagt aattaccagc tatctgattt tctttggtta
    36241 agacaagttt aaacgcccga ccgttattta aatattctga ataatctgct tgcacattcg
    36301 ttgaattttg tcctctgaca atttccagtt cttcttcatt cactaattga tactcctctc
    36361 ctttcttgaa atataggaca tcctgcatag aaaatcctgc atcatttgtg aaaggctgag
    36421 ataattttat cgttaagatc cacggatttt tcgtttctcg ctgatcataa atccataatt
    36481 tttcctgtat atccatttgg ccaaatgctt cttgtgtcaa accagttgct ttgatctgac
    36541 caaaatcgag tttggaaggg gcaagaaatg agagaccacc ttctactacg atcttcgtag
    36601 caggtgcaaa aggcggggca tcatgataaa caactggcgt atcacttgga ttgtgattcc
    36661 caatttcatc gttggtactt ggcttgttct gcacaccctt agcagtagct tctcctgctt
    36721 ggtcgtttaa tacaatttct aatacatctc ctgcaaaaag ttgattttct gggatagaaa
    36781 gaacaaattc ccctgtatca tcaatagttg tttctgacaa aacagcttga ttttttttca
    36841 atgaaaccaa ggctcctgct tcacctgtac ctgtgatcgt gcgagaagct gcattgatca
    36901 atgtggtact taaattagca ggttcaggag gaatcacatc gactgtcgtt ttggcagagt
    36961 ccagttctgt cacttcttgg ccacctctta cttttttagc agcaacaact tgcacttgat
    37021 cctccgtttt taatctctca tcattgggta cttgaacttc gaacaagccg ccttgttcgc
    37081 cttctcccca tgggttatat cctgcccctt cattttcatc tgtttgtcct tgcgtgagag
    37141 cagtaatttc tacagatgat ttcgtcgtat ctgcgtaagt aatacgtaaa gtcacttcta
    37201 cttcgcctgt ccaagcatct cttggctttg cctcgacacc ttcgggaaca acgacatgcc
    37261 catagatttt tttatctgca ttcgttggca ctcttaagga ttcaatcatc gggagctgat
    37321 tattggcact gattcgatta taatttttca ttccggcacc tgtctgagca aattcatttg
    37381 ttaaacctgc actagggctt gatacgaaat tgcctaagtc ggtcccattg atcgataagt
    37441 ctgtcatatc agaaaaataa tccgggtctt tttcaaagtt atcgaatgtt gcgcctgttt
    37501 ttcgccataa agcgatttct gaatgggcga tctttaaaga agaatctcca tttccttgga
    37561 atacccagcc gcctatgcca ttggtctcgc ttccaatccg tttgttttgg aaatcaaaat
    37621 aggtaggaga ttctaagtcc acattcaaca catccccact gatgatgccg gtcttatctt
    37681 cccctgtttt cccgaccatg actaaataag ttttttcacc tgccgtaaaa tttaaatcaa
    37741 tatctccagt tgtatctaca gttgctccgg aatctgcgcg tatatcgata tttgattttt
    37801 ctccagtgac tgtaaaatca gctgaacctg gactttctag aaaaccagaa gcgtcataaa
    37861 attggatcgc ttgatttcct tgattgagtc tgttcacgta agatttgcca tcacctgtat
    37921 ttcttaccct caatgagcct ccattggtta ctgtcagctt ttgcgtacca ttctcaaaac
    37981 gaactgcgct ggaacgccca ctctgcttat cgatgtcgat aactgatccg gctccttcta
    38041 cgatcaaaga aaggcctttt cctacaaaac gcatcccatt gttcccgggt tgatcgtcat
    38101 ctccagccat tttgaccgag atttgtccac cacttgatac atggatttga ctagaatctc
    38161 cttgcaagcg aaatccgccc gtagttgctg tattgatggt gattttccca ccattcgata
    38221 cagtgacttt cgatcctgaa cctctcattt ggatgattcc gcgattgttg tcattggtaa
    38281 tattgatctt tgtatttacc gaaaattcgc ttcccgaacc actgacttca aacatgacat
    38341 taccaggtag cgtatcgact gcctctcctt catcgctaac gccatctgcc cgatcataat
    38401 caagagagac ctttgaaccg cttaagatat ggattcctgg cgctgttttt ccactaccag
    38461 tctcatctcc tgaatctaga aaaccgtaga atttccctag atacggtcca taaagttgac
    38521 taccattgat cacattcaat tgatagcttc tacaaaaata ggctttaccc ggagcggcat
    38581 tcagtccttc cttcactggt tggacatcca acacaccgga aacaccatct aatgtgacag
    38641 ttgcacgagg agcgtaaatc agtcctaatt tggacccatc atacaaatca agtgtcccgg
    38701 taaattttat ttctcccgta aaatgatatc tgcttcccca aaaatcggtc atataagcaa
    38761 tgataatggc atttccatcg atatttggtg tcaatccacc tggatgtgca gctgttcctt
    38821 taaaattctg gaactcgatc gactgaggta tctcattgat ataaatatgg tgtgtgccga
    38881 aattgtgagc gaatccacca aaatcaataa cgatatctcg tttgatctct aattccctgt
    38941 ctaaagtcaa aggctgaatc agtcggatct tagttactga ttcgtctgcg agaagctgcg
    39001 ccagttcttc tgttgtggca gcttcgctgt acccatccgc tgaaacagct cttgccagtg
    39061 ccgcttcact gctatcagac gtttgttcag ctacttctga ggtctcactt gtcttatttt
    39121 cttcttgcct tttattagta gaagcctcac tttgagattc tgtagtttca gaagtaatcg
    39181 attcttctga aaaagtagtc ttgtcaaaac tttgattctc tgattgcgtt gattcgtttg
    39241 ttttctctac atcggcaaaa acggatgtca ccatcaagag agggaagaca aacaaaatcg
    39301 acgcaatcag cttttttaca cattgattca ttctccacca tcctctctga cgttttgaca
    39361 attttatttt atcgttttaa tctatttcca attgtacttt catatttaaa atatgtattt
    39421 ttatggaaaa atttttctat taggtgtaaa tttctttttt gtacaaaaaa aaggctgtga
    39481 caaaagtctt gtcacagtcc cttatcccga acaagcggtg ttctcccgcc aactcctcgg
    39541 tcacaacctc cattttaaca ataaacctgt aaatgtgagg gcaatacttt gattgtcaca
    39601 ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc atcagctaat
    39661 gacacaacta attgctcaca agtaaggtag gctagatttt tcgtagattc atctactcct
    39721 tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt taaataaacc
    39781 aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa accgccgact
    39841 gagttagtta gtcctatcag caaggtactg ctctcaattt cttccttctt tccatcaact
    39901 tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga catgaaataa
    39961 gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattga ttcagggata
    40021 gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat atccaatggc
    40081 ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg tatccctaat
    40141 gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct
    40201 tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat gacaaacaca
    40261 ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt
    40321 gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc taattgctca
    40381 aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac ttttttcatc
    40441 actttctttc ccttctgatt gatttcttta atataacgat agcataaaga aaaaaattag
    40501 gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat aaaacatcca
    40561 tactatgaaa ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa atgataggca
    40621 ttgtatacct aataaagtaa aaatcttagg tggcagataa aaacacaaaa atagcaagag
    40681 aatgtaatag cgtttcttcc actttttgtt atcagacctt atagcgatca gaggaatacc
    40741 aaatgcataa ctgaaaaact cttgatgtac tgttaatatg tcacggcctt gaataataaa
    40801 tgcgacaatc cccgctataa ttagtgtgcc taacaccaaa acggaaaatt gatgaatata
    40861 ttttatatac aaatgatcca ttgtacttcc tgccttttta tttgtgagaa aaacaaatcc
    40921 gtatacgtga ataacttcta gaaataaaag aaatttgcaa aaccactcca tttatctact
    40981 tctacgtgca ttttt
//
LOCUS       DAZKAS010000011        38914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000011 DAZKAS010000000
VERSION     DAZKAS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.011"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..2389)
                     /locus_tag="ACG8H7_000463"
     CDS             complement(8..2389)
                     /locus_tag="ACG8H7_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP8982592.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACG8H7_000464"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="ACG8H7_000464"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFP8982593.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="ACG8H7_000465"
     CDS             3090..3629
                     /locus_tag="ACG8H7_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFP8982594.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="ACG8H7_000466"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="ACG8H7_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFP8982595.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="ACG8H7_000467"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8H7_000467"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="ACG8H7_000468"
     CDS             4736..5902
                     /locus_tag="ACG8H7_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFP8982596.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="ACG8H7_000469"
     CDS             5948..7444
                     /locus_tag="ACG8H7_000469"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFP8982597.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="ACG8H7_000470"
     CDS             7525..8208
                     /locus_tag="ACG8H7_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP8982598.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="ACG8H7_000471"
     CDS             8853..10043
                     /locus_tag="ACG8H7_000471"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP8982599.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="ACG8H7_000472"
     CDS             10145..11098
                     /locus_tag="ACG8H7_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP8982600.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="ACG8H7_000473"
     CDS             11167..12516
                     /locus_tag="ACG8H7_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP8982601.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="ACG8H7_000474"
     CDS             complement(12699..13022)
                     /locus_tag="ACG8H7_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982602.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="ACG8H7_000475"
     CDS             13151..14314
                     /locus_tag="ACG8H7_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP8982603.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="ACG8H7_000476"
     CDS             14384..15733
                     /locus_tag="ACG8H7_000476"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP8982604.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="ACG8H7_000477"
     CDS             complement(15778..16209)
                     /locus_tag="ACG8H7_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8982605.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="ACG8H7_000478"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="ACG8H7_000478"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP8982606.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="ACG8H7_000479"
     CDS             19439..20035
                     /locus_tag="ACG8H7_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP8982607.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="ACG8H7_000480"
     CDS             20157..21305
                     /locus_tag="ACG8H7_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP8982608.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="ACG8H7_000481"
     CDS             complement(21343..21756)
                     /locus_tag="ACG8H7_000481"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFP8982609.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="ACG8H7_000482"
     CDS             21835..22266
                     /locus_tag="ACG8H7_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFP8982610.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="ACG8H7_000483"
     CDS             complement(22365..22598)
                     /locus_tag="ACG8H7_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8982611.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="ACG8H7_000484"
     CDS             22781..24451
                     /locus_tag="ACG8H7_000484"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFP8982612.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="ACG8H7_000485"
     CDS             24598..24984
                     /locus_tag="ACG8H7_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982613.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="ACG8H7_000486"
     CDS             24971..25669
                     /locus_tag="ACG8H7_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFP8982614.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /gene="lspA"
                     /locus_tag="ACG8H7_000487"
     CDS             25674..26138
                     /gene="lspA"
                     /locus_tag="ACG8H7_000487"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP8982615.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="ACG8H7_000488"
     CDS             26135..27046
                     /locus_tag="ACG8H7_000488"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP8982616.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="ACG8H7_000489"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="ACG8H7_000489"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFP8982617.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="ACG8H7_000490"
     CDS             27834..29117
                     /locus_tag="ACG8H7_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFP8982618.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="ACG8H7_000491"
     CDS             29130..30056
                     /locus_tag="ACG8H7_000491"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFP8982619.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="ACG8H7_000492"
     CDS             30075..31355
                     /locus_tag="ACG8H7_000492"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP8982620.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /locus_tag="ACG8H7_000493"
     CDS             31360..32436
                     /locus_tag="ACG8H7_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFP8982621.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="ACG8H7_000494"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="ACG8H7_000494"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP8982622.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="ACG8H7_000495"
     CDS             35680..36456
                     /locus_tag="ACG8H7_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFP8982623.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="ACG8H7_000496"
     CDS             36453..37385
                     /locus_tag="ACG8H7_000496"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFP8982624.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="ACG8H7_000497"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="ACG8H7_000497"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP8982625.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38731
                     /gene="pyrE"
                     /locus_tag="ACG8H7_000498"
                     /pseudo
     CDS             38105..38731
                     /gene="pyrE"
                     /locus_tag="ACG8H7_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
BASE COUNT        12516 a         6895 c         8490 g        11013 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aaga
//
LOCUS       DAZKAS010000012        36128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000012 DAZKAS010000000
VERSION     DAZKAS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.012"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..847
                     /gene="purR"
                     /locus_tag="ACG8H7_000499"
     CDS             29..847
                     /gene="purR"
                     /locus_tag="ACG8H7_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFP8982626.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1350..2723
                     /gene="glmU"
                     /locus_tag="ACG8H7_000500"
     CDS             1350..2723
                     /gene="glmU"
                     /locus_tag="ACG8H7_000500"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP8982627.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2817..3788
                     /locus_tag="ACG8H7_000501"
     CDS             2817..3788
                     /locus_tag="ACG8H7_000501"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP8982628.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3794..3896
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3962..4531
                     /gene="thiT"
                     /locus_tag="ACG8H7_000502"
     CDS             3962..4531
                     /gene="thiT"
                     /locus_tag="ACG8H7_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFP8982629.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4612..5385)
                     /locus_tag="ACG8H7_000503"
     CDS             complement(4612..5385)
                     /locus_tag="ACG8H7_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982630.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5652..7352
                     /locus_tag="ACG8H7_000504"
     CDS             5652..7352
                     /locus_tag="ACG8H7_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP8982631.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7322..7540
                     /locus_tag="ACG8H7_000505"
     CDS             7322..7540
                     /locus_tag="ACG8H7_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFP8982632.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7560..8450
                     /locus_tag="ACG8H7_000506"
     CDS             7560..8450
                     /locus_tag="ACG8H7_000506"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP8982633.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8468..8752
                     /locus_tag="ACG8H7_000507"
     CDS             8468..8752
                     /locus_tag="ACG8H7_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982634.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9239..9853
                     /gene="gmk"
                     /locus_tag="ACG8H7_000508"
     CDS             9239..9853
                     /gene="gmk"
                     /locus_tag="ACG8H7_000508"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP8982635.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9858..10163
                     /gene="rpoZ"
                     /locus_tag="ACG8H7_000509"
     CDS             9858..10163
                     /gene="rpoZ"
                     /locus_tag="ACG8H7_000509"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP8982636.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10416..12824
                     /gene="priA"
                     /locus_tag="ACG8H7_000510"
     CDS             10416..12824
                     /gene="priA"
                     /locus_tag="ACG8H7_000510"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP8982637.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12839..13330
                     /gene="def"
                     /locus_tag="ACG8H7_000511"
     CDS             12839..13330
                     /gene="def"
                     /locus_tag="ACG8H7_000511"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP8982638.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13323..14261
                     /gene="fmt"
                     /locus_tag="ACG8H7_000512"
     CDS             13323..14261
                     /gene="fmt"
                     /locus_tag="ACG8H7_000512"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP8982639.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14261..15619
                     /gene="rsmB"
                     /locus_tag="ACG8H7_000513"
     CDS             14261..15619
                     /gene="rsmB"
                     /locus_tag="ACG8H7_000513"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP8982640.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15632..16372
                     /locus_tag="ACG8H7_000514"
     CDS             15632..16372
                     /locus_tag="ACG8H7_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFP8982641.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16369..18438
                     /gene="pknB"
                     /locus_tag="ACG8H7_000515"
     CDS             16369..18438
                     /gene="pknB"
                     /locus_tag="ACG8H7_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFP8982642.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18603..19502
                     /gene="rsgA"
                     /locus_tag="ACG8H7_000516"
     CDS             18603..19502
                     /gene="rsgA"
                     /locus_tag="ACG8H7_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFP8982643.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19515..20165
                     /gene="rpe"
                     /locus_tag="ACG8H7_000517"
     CDS             19515..20165
                     /gene="rpe"
                     /locus_tag="ACG8H7_000517"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP8982644.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20166..20804
                     /locus_tag="ACG8H7_000518"
     CDS             20166..20804
                     /locus_tag="ACG8H7_000518"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFP8982645.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20959..21813
                     /gene="mreC"
                     /locus_tag="ACG8H7_000519"
     CDS             20959..21813
                     /gene="mreC"
                     /locus_tag="ACG8H7_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP8982646.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21817..22326
                     /gene="mreD"
                     /locus_tag="ACG8H7_000520"
     CDS             21817..22326
                     /gene="mreD"
                     /locus_tag="ACG8H7_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP8982647.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22581..24155
                     /locus_tag="ACG8H7_000521"
     CDS             22581..24155
                     /locus_tag="ACG8H7_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP8982648.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(24223..26022)
                     /locus_tag="ACG8H7_000522"
     CDS             complement(24223..26022)
                     /locus_tag="ACG8H7_000522"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFP8982649.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            26145..26651
                     /locus_tag="ACG8H7_000523"
     CDS             26145..26651
                     /locus_tag="ACG8H7_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP8982650.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            26730..27452
                     /locus_tag="ACG8H7_000524"
     CDS             26730..27452
                     /locus_tag="ACG8H7_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8982651.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(27530..28930)
                     /locus_tag="ACG8H7_000525"
     CDS             complement(27530..28930)
                     /locus_tag="ACG8H7_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFP8982652.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(29100..30074)
                     /locus_tag="ACG8H7_000526"
     CDS             complement(29100..30074)
                     /locus_tag="ACG8H7_000526"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP8982653.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            30427..30987
                     /gene="pth"
                     /locus_tag="ACG8H7_000527"
     CDS             30427..30987
                     /gene="pth"
                     /locus_tag="ACG8H7_000527"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP8982654.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            31004..34525
                     /gene="mfd"
                     /locus_tag="ACG8H7_000528"
     CDS             31004..34525
                     /gene="mfd"
                     /locus_tag="ACG8H7_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP8982655.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            34540..>36128
                     /locus_tag="ACG8H7_000529"
     CDS             34540..>36128
                     /locus_tag="ACG8H7_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP8982656.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
BASE COUNT        11710 a         6611 c         7775 g        10032 t
ORIGIN      
        1 taaaatttct taaaaggagt tggaataggt gaaagtacgt cgcagtgaac gtctagttga
       61 catgacacag tatttattga atcacccgca tgaattgatt tccttaacaa gctttgccga
      121 acgttatgaa tctgctaaat cttctatcag tgaagatttg ggaatcatca agaaaacatt
      181 taaagaacga gggtatggaa ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt
      241 catccctgaa atttcatatg aagaggcgaa aaaatatatt gaatctctag cagaacgtct
      301 atcagaacaa gaccgtctgc ttccaggagg atatgtttat ctctctgatt tgttaggaga
      361 acctgaactg ctgcgtcaag ttggaagaat cattgcttct aaataccttg gtgaaaaaat
      421 cgatgctgta atgactgtag ccacaaaagg cgtacctatt gcccaagctg tgtcctatta
      481 tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa ataacagaag gatcaacagt
      541 aagtgtcaac tatgtgtctg gatcttctga aagaattgaa aagatggaac tgtctaagag
      601 aagtttgaaa cgtggttcac gtgtccttgt agtggatgat ttcatgaaag gcggcggaac
      661 agtcaacgga atgaagagct tgattgaaga attcgaatca gaacttgttg gcatcactgt
      721 atttgccgaa tcaaaattca acggtcatcg cgccatcgat gattatacgt cactgcttta
      781 tgtcaaagat gtagatactc aaacgaaaaa tattacggtt gtgccaggaa attatttttc
      841 agaataagag gttgtgaaaa agccgcttag tttcgagtag taagaaaaat tttgtgaaaa
      901 tggctttcca cattttttca taaatttggc ttaataccga gaaactggct tttgaacacc
      961 gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa taaacggaaa
     1021 aattgctcct catattttgc cgtttattga gctaataccg aaggtcagct gattgaaaaa
     1081 cgtttaatga agaggttgta agcctaccgt ttaactctaa gtcacaagaa acgatttcaa
     1141 aaaacagctt ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt ggcaggagag
     1201 caccggttac caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc
     1261 tgggataaaa gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa
     1321 tcagcaaact atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg
     1381 cagggaaagg tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta
     1441 aacctatggt agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta
     1501 cgatcgttgg acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg
     1561 cgttacaagc agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg
     1621 aaggaaaaaa aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa
     1681 cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg
     1741 cacaagcaga aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag
     1801 aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata
     1861 caggaactta ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa
     1921 acaatgctca aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc
     1981 atacagttgc tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga
     2041 tcgcgttagc taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca
     2101 atggcgtaag ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc
     2161 cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt
     2221 gtgtcatcgg gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg
     2281 aaaactcagt cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac
     2341 atttacgacc aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga
     2401 aaaatgctca aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta
     2461 cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga
     2521 acaaacatca tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg
     2581 gacctgtcga ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata
     2641 ttccagaata tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa
     2701 aaaaactccc ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat
     2761 gactactatt tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt
     2821 caaaacatta ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag
     2881 cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta
     2941 gtgatggaga aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta
     3001 ttcaatcaac gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg
     3061 cactgaaacg agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc
     3121 gtcaagaccg taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga
     3181 ttcaaaaagc tggtgcaaca agaatgctga ctttggacct tcatgcagtc caaatccaag
     3241 gtttctttga tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct
     3301 tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca
     3361 cacgggcacg taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc
     3421 gtccaaaagc aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt
     3481 gtgtactgat cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt
     3541 tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc
     3601 ctgctttaca aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct
     3661 atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg
     3721 gagatgcaat caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa
     3781 ataaataata aataaactca caaaggggag tccaattggg ctgagattga atctatttct
     3841 aaacccttcg tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg
     3901 actattttcg aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt
     3961 tatgggaaaa aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt
     4021 gtcgttgatt ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt
     4081 gactcttttt gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg
     4141 catcctacat tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga
     4201 gtacccgatt gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa
     4261 aggctatgta gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat
     4321 tggcgcactt tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta
     4381 tgcactatgg gggatgaatc cttggctctt ttctttcgta atgaatggtg ccagcgggtt
     4441 gagtacagct attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat
     4501 ttttgtgcca gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag
     4561 aatcatgtcc tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa
     4621 tggcagataa gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta
     4681 gattaaagta gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata
     4741 atcttcaatc acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa
     4801 agaaaaaatg atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat
     4861 tttttcaaca ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa
     4921 gatcgaactt aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat
     4981 ccgattcagc tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa
     5041 cggcgaatga agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg
     5101 tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc
     5161 acgcctgtta cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt
     5221 cagccctgta tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt
     5281 ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac
     5341 agcattactt gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt
     5401 atttcctctt aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat
     5461 tttaaaattc cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt
     5521 ttgtgatttt tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc
     5581 tacaaagtcg aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa
     5641 aggagagcgc gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc
     5701 ctatccagca agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg
     5761 ctttttggcg ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg
     5821 tctattttac tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag
     5881 gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta
     5941 ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt
     6001 ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag
     6061 cacttgtttt aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc
     6121 tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg
     6181 gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga
     6241 caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct
     6301 atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc
     6361 cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa
     6421 tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc
     6481 aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg
     6541 ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa
     6601 tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag
     6661 ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa
     6721 cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac
     6781 aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat
     6841 ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag
     6901 aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt
     6961 tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa
     7021 aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct
     7081 gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt caattaagta
     7141 ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa
     7201 ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc
     7261 ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac
     7321 catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa
     7381 caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga
     7441 tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga
     7501 agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa
     7561 tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata
     7621 ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc
     7681 tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa
     7741 tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact
     7801 gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta
     7861 gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgtgctggac gaaaacttcg
     7921 taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata
     7981 cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag
     8041 ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt
     8101 ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc
     8161 ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg
     8221 gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta
     8281 tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg
     8341 aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt
     8401 ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt
     8461 agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa
     8521 acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta
     8581 taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa
     8641 atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg
     8701 aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg
     8761 ctgaacaaag aaatacaata aataaacaga attggatttc ttcgcttagc ttctttgctc
     8821 taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata
     8881 agtgatgttc aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa atgtgagaaa
     8941 ctattttcac aaaatttttc ttcatactcg aaactaatcg gcttcttcac gacctcttat
     9001 acacggcgtt caaaagcttg catctgcgat aataacccca accaaacgaa aaacatgaga
     9061 agctgtttat gtttggctgt tcttattact tgatgcagga cagctttttt acgacctctt
     9121 ggtacagggt agctttttta ccaatttcta gctataatta aagataacct tgaaagttgc
     9181 agaaaaaaag tgcaaaataa acgataagta caaaaagact agttgaaagg acgtcgtcat
     9241 gtcagagcgt ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa aaggcacagt
     9301 acgaaaagca atttttgaga gtgaggataa tgattttcag tattcgatct caatgactac
     9361 ccgtaaaatg cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa gcaaagaaga
     9421 gtttgaatca atgatcgaag ctggagagat gctggaatac gcagaatatg tcgggaatta
     9481 ttatggaact cctttgtcat atgtcaacaa gacattggat gaaggaaaag atgttttctt
     9541 ggagattgaa gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg gtgtattcat
     9601 ctttttgact ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc gaggaacaga
     9661 tgcagatgaa gtaatcgaaa aacgaatgaa agttgcaaaa gaagaaatcg aaatgatggc
     9721 tctttacgac tatgctgttg taaatgatga agtgccgcta gcagttgaac gaatcaaaaa
     9781 cattattgcc agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata ttaaaatgtt
     9841 aaaggagatg tagtcttatg atgttaaaac cttcaattga ctcactatta gatcgtgtaa
     9901 attcaaaata ttctctagtg attttagcaa gtaaacgcgc gcatgaattg gatgcaagtg
     9961 cacagcctac tctagattct tttgattcag taaaaagcgt cggtcaagct ttagaagaaa
    10021 tcgatgcagg aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa cgtttgaaga
    10081 tggaagaaga ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg gaatcacgta
    10141 tccgtgaaga gcaaaaaatg taataaagaa aaatagagcg aacatctaaa aagaaaagca
    10201 aacccagctg tttccttttc aaacattcgc cacttattgt ctggtagaaa aggctgtgtc
    10261 tgaacttttt gacacagttc ttttttttct tatctgctgt taattttttg ttaggatttg
    10321 gcagaagata agacaaatcc atcgatctct agtaaaatag gcaaggaact ttttgtcgct
    10381 tgataagaaa agacaaaaaa aggaggaaac gaaccatgac attaacagct gaagtcattg
    10441 tggacgtacc tactatgcag accgatcagc cattcactta tttggttccc tctgaaatgg
    10501 aaacagcttt gcaagtcggg atgcgagtag aagtcccttt tggtaatggc aatcgtcatg
    10561 tccaaggatt tgttatggct atccaaaaat cagaagaatc agctaatcca tctttgaagg
    10621 cagttattcg tttgctagac cttgctccgg tggtcaatga agaattactt tcattagctg
    10681 attatatgaa aaaaattact tacgcattca aaatcacttg tctacaaacg atgcttccga
    10741 gtgtgatgaa agcagaatat gataaattga tctatccttt ggcagacaca ccagaagttc
    10801 aggtattgtt tgatcagcga gaagtgattt cctggaagga agcagaagaa gaaggcagcc
    10861 tttctcaatt gattcgctgg cgccaagagc aattagtaga tatcaaatat gaagtccata
    10921 cacgaaataa agtaaaaacg attcgtttgg tccgttcttt gttaactgaa aagcaaatcg
    10981 aagaagaatg ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt cttttgtgtt
    11041 tatctgaact gagccaagaa gaaccaattg cttatttcaa aaataaagga atcagtactg
    11101 ctgtcttgaa tcaaggaaaa gaaaagggct ggctggagtt tatagaatca gaacgttatc
    11161 gcgaccctta taaagaccga gtatttgacc aaacgacggc attggaactg aatgcagaac
    11221 aaaaaaatgc tgtcgaacaa atcatcacag caggtcagca gcaaaaagat caggtctttt
    11281 tgctggaggg aatcactggc agcgggaaaa cagaagtata tcttcaggca atcgctgatg
    11341 ttttgtcaga aaataaaacg gctattatgt tagtgcctga aatcgcattg actccgcaaa
    11401 tggtcgagcg attcaaagga cgcttcggag aatcagttgc ggtcttacat agcggacttt
    11461 cccaaggtga aaaatatgat gaatggcgca agattgagcg ggaagaagcg caagtagtag
    11521 taggcgcacg atctgctatt tttgccccgc tgaaaaatat cgggctgatc attatcgatg
    11581 aagaacatga atcaagttac aaacaagatg agacaccgcg ttatcatgcg agagatctag
    11641 caatttggcg cagtaaatat catcattgcc caatagttct aggaagcgcg acgccctctt
    11701 tagaatcaag agcacgagct caaaaaggcg tttatcagtt attgcagtta aaccaccgag
    11761 ccaaagcagc tgcacagctt ccagctattg agatcgtaga tatgcgtgaa gagtttcaga
    11821 atcatcggac ctctactttt tctgctaatt tgcaggaaaa aatacaaaat cgattagaca
    11881 aaaaagaaca aactgtccta ctgctcaatc gtcgaggata ttcgtctttt gtgatgtgta
    11941 gggactgtgg atttgttctt ccatgtccta attgcgatat ctctcttaca ctgcatatgg
    12001 atacacgttc aatgcgctgc cattattgcg gacatgaaga acctattccc aatcgctgtc
    12061 ctaattgtgg cggcaataaa atccgctact atggaacagg tactcaaaaa gtagaagagg
    12121 aactccgaga actttatccg caagctagga ttttgcgtat ggatgtagat accactcgaa
    12181 gaaaaggggc acacgaacag attttacaaa aattcggtgc aaaagaagca gatatattat
    12241 tagggacaca aatgattgct aaaggtttgg actttccaga agtgacatta gttggggtgt
    12301 tgaatgctga tacctccctg aatttaccgg attttcgttc tagtgagcat acattccaat
    12361 tgcttaccca agtttcagga cgagcagggc gagcggaaaa agcaggcgaa gtcgtgatcc
    12421 aaacattcaa tcctcaacat tacgccatag aattggctaa aaaacaaaat tatgagcagt
    12481 tttatcagca ggaaatgcat gtacggcatc gtggaggcta tcctccttat tactttactg
    12541 tgaaaataac cgccagccat cctgaagaac aagtagcggc aaagaaaatc tttcagattg
    12601 ccaatcagct aaaagaagtg ttaagccctc aaagcttgct attagggcca acgcccagca
    12661 tgattttgag agtgaaaaac cgatattatt atcaactaat catcaaatat aaacatgaac
    12721 cgaatcttcc tcatgttttg gatgaaattt tgaatggaag ccagaaagaa cagcgacaag
    12781 gtctttttgt ggcaattgac aacgaaccat taaactttat ttaagaaagg gccaaattat
    12841 gcgctatcct atattgatcc accctaatga taaattaaaa agaactgctc agccaatcga
    12901 tgtaatcacg gacgagacaa tcgctttatt agacaattta tatgagacaa tgattgcgaa
    12961 tgacggaata gggatcgctg ctcctcaagt aggacaaaac aagagaattg ctgtgatcga
    13021 agtagatgaa ggcgagaaat ttgaactgat taacccagaa atcatcgaag caaaaggtga
    13081 aagccttgac gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg tcaagcgagc
    13141 agatgaagtg accgtgcgtt attatgatcg agacggagaa gagatagaag tgacggcttt
    13201 tggctattta gcccgtgcct tccagcatga aatcgatcac ttagacggta tcttgttcat
    13261 tgagaaaatg atacagcaga tcccggaaga agaacttgaa gaatatatgg aggaacatct
    13321 tgatgactaa gctagtattt atgggcacac ctgctttttc tgtgccgatt cttgaagggc
    13381 tgatagaatc aggttacgag atccaggcag tggtaacaca gcccgaccgt ccagttgggc
    13441 ggaaacgagt aattacgccg acaccggtaa aaaaagcggc tctgaagcac ggtatccgtg
    13501 tgctgcagcc agagaaaatc tccggttcgc cagaaatgga agaaatcatt gaattggtgc
    13561 ctgatgtgat cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta ttgcaagtac
    13621 caaaattagg ggcaatcaat gtccatgctt ctttacttcc taaatatcgg ggaggtgcgc
    13681 ctgttcatta tgccatcatg aatggggaga aagaaactgg tgtaacgatc atggaaatga
    13741 tcaaaaaaat ggatgcaggc ggaatctatg cgcaagaaag cataccaatc actaaacaag
    13801 atgatgtagg gacgatgttt gaaaagctta gccttttagg aagaaagcta ttgctagaaa
    13861 cattgcccaa tattttggat ggacaaaaac cagtacctca agacgagtca gaagcaactt
    13921 tttcgccaaa catcacacgg gaacaagaag caatcgattg gcataaaact gcagaagaaa
    13981 ttgacaacca agtacgcgga atgcgtcctt ggccaattgc attcacgacc tatgagggca
    14041 tacgctggaa attattatcg gttgaagtat tggcagaaac tacagacaaa gcacctggta
    14101 cgatcatccg gaaagacaaa aaaaatctat ggattgcttg cggtaaacag accgtcttag
    14161 ccatcaatga attacagcca gctggtaaag gaaaacaggc cgtacatgat ttccttaacg
    14221 gaagcggtca gcaagttcag attggtcagc aggtgaaata atgaaaaaaa tccctaaaaa
    14281 aatgaaaaaa tcagtacgat atatggcatt actgacatta gagcgagtga acaagggtgg
    14341 cgcctactcg aatcttttgt tgaatgagat gatcaaacaa agcgagctaa atcaaaaaga
    14401 tgtgggttta tttacagaat tgatctatgg tacgattagc catcagcgac tattggaatt
    14461 ttacgttgca ccattgatcg ctaaagcaaa aaaagtggat gactgggtca agatgcttct
    14521 ttatctttct gtataccaat tagaattttt agataaagtc cctgcacatg ctattttaaa
    14581 cgaggcggtc gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat ttgtaaatgg
    14641 tgtcttgagg aattatcagc gtcaaggagc accagatctg caaggcattg ctgatcctat
    14701 cgaaagactt tctgtcaaaa tcagcctgcc tctatggatg acgaaacgac tggtggatca
    14761 aatcggatac gaagaaactg aaaagctcgg tttatcgcta tatcagccta gccatgctag
    14821 tgcccgagta gatacacgcc gactttccag agatcaggcc atcactgttt tgcaagaaga
    14881 agagatcaca gctgaaaaaa gccaagtcag tccatacggt atagtagcga aaaaagggca
    14941 tttagcttct agctcattgt tccacgatgg cgtcatgacg atccaagatg aaagttcgat
    15001 gcttgtagcg ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg cctgtgcagc
    15061 acccggaggt aagacgacgc atattgcttc ttttttagat ccgtcatgcg gtggagaagt
    15121 ggtcgcttta gatgtacacg aacacaaagt caaactcatt gaagataatg cagcacgtct
    15181 aggtgtctct tctgctgtct acgctcaaaa gatggatgct agagaagtca gtaaacagtt
    15241 cgaagcagag cagtttgatc ggattttagt agacgcgcct tgttcaggtc ttgggctgct
    15301 tcggcgaaaa ccagatattc gttataaaaa acagccggat gaattagcga atttaccaaa
    15361 aatccaattg gaaattttag aaagctgcgc accaacttta aagtcttccg gtattctgac
    15421 ttatagcact tgtacgatct taaaagaaga aaaccaagac gttgtttctg ctttcttgga
    15481 aagacaccct gattttgaga ggattgatgt tttgaccaat cctatgctac aatcatcaat
    15541 cgaagagaaa atgttgactt tgtatccgca tcagttttac acagatggat ttttcatttg
    15601 ttgtttgcgc aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat cagatgtagg
    15661 aagacgacgt aacacaaatc aagactatgc cagtgtattt accagccgtt caggtctcaa
    15721 gcttgctgta ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg ccagtcagat
    15781 ggcggtaacg aatctaggcg aagcttggga aaaacaagaa gctgttgacg atgaaaaaat
    15841 cgcccaatgg ttcattcaat cgattcaaga agaaaatatg ctgatttatc agcgtggaca
    15901 agaacagccg gaatataacg gtatgggaac aacaatcgta gcggccgctc tgtcagatga
    15961 ctggttcacg attgccaatg ttggggatag cagagcttat ttgattcgtg aagataaaat
    16021 catgcaattg acggaggatc attctttagt caatgaacta gtcaaatcag gagaaatcag
    16081 cgaagaaatg gcagtgaacc atccaaggaa aaatatttta acacgttctg tcggcatgcc
    16141 tggaacagtc gaagtagatg tttctactca tctttggcaa ctaaaagatc gactgctgtt
    16201 atgttcagat ggcttgacga acatgctgtc tgaagaagaa ataggaacga tcatcaatca
    16261 agaaggccca ttgtctcata aagtaactga gctgattgat cgtgccaatg aagcaggcgg
    16321 agcggataat atcaccgttc ttcttatcga atataaggag gacagtgtat gatcgagttg
    16381 ggcaaaaaat taaatggccg ttatcaaatc actggaaata tcggtagcgg tggtatggca
    16441 aatgtatttt tggcgcacga tttaatcttg gatcgtgatg tagcagttaa agtcttacga
    16501 tttgactttc aaaatgacca gactgcgatt cgacgatttc agagagaagc tttagcagca
    16561 acagaactcg ttcatccaaa cattgttagt gtttacgatg ttggtgaaga agacaacatg
    16621 cagtatctgg tgatggaata cgtcaaagga atggacttga aacgatatat ccaaacacat
    16681 tatcctgttc cttatgaaac agcagtaaat attatgcagc agatcctttc tgctatttct
    16741 ttagctcata accatcaaat catccaccga gatttgaaac cccaaaacgt actgatcgat
    16801 aatgaaggtg ttgtaaaaat cacggatttc gggattgcta tcgctttatc tgaaacatcg
    16861 atcacgcaga cgaatacgat gctaggatct gttcattatc tgtcccctga gcaggccaga
    16921 ggaagtatgg caacgaagca gtcagatatt tatgctttag ggatcatcct ttatgagatg
    16981 ttgacgggta gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt aaaacatttt
    17041 caagatgatc ttccatctat aaaagcactt gatccaaatg tgccacaagc actggaaaac
    17101 gtgattttac gggcaacggc taaagaacct gcagaccgtt ataagtcagc cgaggaaatg
    17161 tctgatgatc tttctacagt attatcacca gctcgtgcaa acgaagaaaa atggcagcct
    17221 catgtgatgg ataatgaaac aaaagttatc acacccctta cggaagatgc accaatgcct
    17281 gattcattca aatctatgcc tctgccaaaa gacaagccag aaaacgaaac cttaccagag
    17341 gaggaagaag ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg gctgatccta
    17401 ttagctattc ttgctgtttt aggaatcgga acagctgtat tctttgcttc tggcgggcga
    17461 ggagaagttg ttgttcctga tgtttctgat ctttctgaag cttctgctag agaaacactc
    17521 gcaaaagcga aattagaagt agcaacaaaa acagaagaaa ttgcagatga caaaatcgaa
    17581 gaaggtaatg tagtgaagac agacccggca gcgggaacga cagtcaagca aaaacgcgaa
    17641 gtcactctgt atattagtag cggcacgaaa aaaatcaaat tagatgatta cagtggtaaa
    17701 agttatgaag atgccgaagc aagtttgaaa gaattagggt tttctgctga cttgattcaa
    17761 aaagaagagg aattcagttc agaggtagaa agcggaatga tcatttcgca atctccatca
    17821 gaagatacag aagtcgatcc taaaacagac acgattactt tcacagtcag caaaggacca
    17881 gaaacagtga ctttgagtag tcatgtagga gaaaactacg aaactgttcg taattggctt
    17941 gttacacaag gattcaatat caataatatt agtgctactt atgactatag cgacactgta
    18001 ccatcaggtt cgatcatcag tcaggatcca gcctcgggtg aagttgtagc agaagaaact
    18061 tacatcaatt ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat ctccggctat
    18121 acaaaaagcg aagcacaaag ttatttagct agtgtcggtg cagaatatat cgggcatgag
    18181 acttccgaat attcaaacac ggtggataaa gataaagtga ttcgtacagt accaggggca
    18241 ggaacgacaa ttaccaaagg aatggttgtg aacgtaattt actctaaagg accagatcca
    18301 gcgtcttcta gttctgaaaa agactcttca gacagttcta gcagctcaag tagctccagt
    18361 agcacaagca actcttcaaa agaagaatct tccagctcca caagttcatc agctagcacc
    18421 tcagaaagtt caaaataagt gctgaataaa atatatcaga agggaatgcg acattgattt
    18481 gttgcattct cttttttgtg tttctatctc ttttgatcaa ttttcaagcc atatactttg
    18541 agaagtttgc atggaataac acctactttc ctcttcgtat ggtaaaataa acaaaaacgg
    18601 aggtggcact tatcaaagga caaatcagaa aagcaatcag cggtttttac tatgtatacg
    18661 caaacggaga gacctatcaa acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc
    18721 cgcttgtcgg agatcaggtg atctttgaga gtgaaaatca aacagacggc tatcttctgg
    18781 aaatcttgcc gagaaaaaat caattagttc gcccaccagt cacaaatgtt gatcttggaa
    18841 tcattgtcac aagtatcgta gagcctgcat tttcttataa tctacttgac cgctttctag
    18901 ttaccttgga atacgagtcg atcgaaccga ttattttttt gacaaagacc gacttgtcag
    18961 acgacccaga aaaaatcgaa gagatcaagc aaacctatac taaaataggc tatccggtga
    19021 ttgtacccaa tcatacgggt gatacgacag aactgatccg ttacttccca gaacggctat
    19081 cggtctttat gggtcagtct ggagcaggaa aatcgacgct actgaatcaa atatcaccgg
    19141 aactgaattt agcaacagga gagatttctg atgcacttgg aagaggacgg catacaacac
    19201 gtcatgtgga actgattcct atctatggcg ggttagtagc agatacacca ggatttagct
    19261 cgattgactt tttgacaatt gaagcaggag aactgccgag acaatttcct gaatttgtag
    19321 aagcttctgt ccattgcaaa tttagagagt gcatgcatgc taaagaacct ggctgcgaag
    19381 taaaacatag agtagaaaca ggtggtatcg cacagacacg ttacgacaat tatttgcaat
    19441 tcttacaaga aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat
    19501 aggaggaaac aagaatgaag atagcaccat ccattttgag tgcagatttt gccaatttac
    19561 aaagagacat tgagttagtg gaaaaaggag gcgcagatta catccacgta gatgtaatgg
    19621 atggccgatt tgtcccaaat atcacattag ggccaaatat tgttcaggca atccgtcctg
    19681 ttactaaatt accgttagac gtccatctga tgatcgttga tccagaaaac tacattccag
    19741 catttgcaaa agctggagca gatatcatta ctgtccacgt cgaagcaact cctcatattc
    19801 atcgggcact tcaaatgaca aaaagcttag gagttaaagc tggagtagtt atcaatccag
    19861 gaacaccagt ctccatgatc aaacatgttt tgcctttggc tgatcaagta ctagtcatga
    19921 ctgtcaatcc tgggttcggc ggacaaagct ttattgaaga aacagtagaa aaaattacgg
    19981 aactcgctga tctacgtaga gagaacaatt ggacttatgc aatcgaagta gatggcggaa
    20041 tcgttccaga aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc gttgcaggat
    20101 catatatcta tggagccgaa aatccagtcg gacaaatcga acgattaaaa gaggcattag
    20161 actaaatgaa aattttatta gctgctggag gtaatccgca aaactggccg cagtctctgc
    20221 tgtccaaata cgatgcctat gtcgggatag accgtggtgc gctgtacctg cttcaagcag
    20281 gctacccagt agatctggca gtgggtgatt ttgattcatt gtcgaacgaa gagcagaagg
    20341 aagtatttgc aaaagccaaa gagtataccc gttcccctgc agaaaaagac gatacagata
    20401 cacagttagg tatcttaaaa acatttgaac gttacccgca agcggaagtc actttgattg
    20461 gagctacggg cggaagattg gatcatcttt tagccaatct ctggttaggt atggaacctc
    20521 gcttcaaacc ctttgcccat cagcttatta tcagagattg tcaaaattat ttgtcttatt
    20581 atttgcctgg agagcatata gtaagacaga taccagatat gaagtattta gcttattgct
    20641 gcttgactcc cgtctcgaat cttacattaa aaggcagcaa gtatgtattg gaaaataaag
    20701 aagtagaata tccgacatct tatgcaagta acgaatttct ttcagatacc gcagaatttt
    20761 ctttttcaga tggtatgatt gcagttattc aaagcaaaga ttgatccata aacaaataaa
    20821 aaaatttgtc tttcaggcta aaaatgacag aatctattat ttgctaaaaa aaagcttaag
    20881 aggagcaact taccttttgt ttcacggcat ttagtgataa aattgaacac aagagtctaa
    20941 ttaattcgag gtgtctttgt gaagaagttc aatcctaata aaaatattat tattaccttg
    21001 gttattgtta tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa
    21061 ggaaagccca atttcttcca atccatcgtt aatgacagtg ttgcaatggt tgataaaaca
    21121 atctctgccc cagtacggtg ggtacgaaat ggagtagatt ctgtccataa tcttttttca
    21181 acttatgccg aaaatgagcg gctaaaagaa aaaatcgata gctatgatga cgtttcacag
    21241 caaaacaaga attacgagag agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag
    21301 acgctgacta gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc tgacagttgg
    21361 caggacatgc tgatcgtaga caagggttct aaagacggta tcgaagcaaa tatggcagtc
    21421 atgtcgcaaa aaggattgat tggccgagtg atcgaggtca atacggcttc gtctaaaatc
    21481 gaattactgt catcctctaa tgaaagctcc aatcattttc cagtacgggt atcttcggct
    21541 aatggcgaag cgtttggttt gcttaaaaac tatgatgaaa agctccatgc cttagtggtg
    21601 acccaattaa ctggtgatac ggatatcaaa gaaggggatg ttgtccagac atccggtctt
    21661 ggagggaatt ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa accagatagt
    21721 tatgggctgg atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt
    21781 gtgacgattg tccaacggct agcggaggat tagtccatgt tgaagaaaga aacaatgaag
    21841 tactatctgc caatcgtttt gttctttttg atgttgatag atggtcattt aacaagaatg
    21901 ctaggggagt ggtcgaaagg cacctatatg tcaaatgccc actttctgat attggcatta
    21961 ttatgttgca gtatggcgtt tgaaaaacgt tatttactga ttaccacgat tgttctcggg
    22021 gctatctatg acgcttacta tattggcgtt atcggtatct atgcagtagc tctcccttta
    22081 attgtatggt tgatgtatgt aatgaaagac gttatccatg tcaacatctt tactgaattt
    22141 ttcagtatga tcatctttgt cacgggttat gaattgttta cgatggtggt ccagttgatt
    22201 tttaaattag cagtagtaaa taacacgtat tttattacaa ggtttttagg acctacactg
    22261 ctgttgaaca tgattatatt tgtattattc atttttccct ttaagaaatt attcagtgat
    22321 gaataatttc tttttttatt tttaggtcga aagcgaaaaa gaatcgttgt tatcttaaga
    22381 aatgctagac ttaacgcggc tttttcatga tcattttctg aaacgaacag ttttgtcatt
    22441 tttaaaagtt gttttgaaac atttttgtaa cataactatt atatcgttga cacactgcta
    22501 tggtacaatt tttatatcgt taagaaagac ttaaatatat ttagatattt attaaattaa
    22561 gccagtcgga ggaatgaaga gtgaaaaaga gtttaatatc agcagtaatg gtatgttcaa
    22621 tgaccttgac tgccgtagca tcaccgatcg ctgccgcggc ggacgatttt gattctcaga
    22681 tacaacagca agatcaaaag attgcagact tgaaaaatca acaagcagat gcacaatcac
    22741 aaatcgatgc cttagaaagc caagtttctg aaatcaacac acaagcacaa gacctattag
    22801 caaaacaaga tacattgcgt caagaatctg cacaattagt aaaagacatt gcagatttac
    22861 aagaacgtat tgaaaaacgt gaagatacaa tccaaaaaca agcacgcgaa gcacaagtaa
    22921 gcaacacaag ctcaaactac atcgatgctg tattgaatgc tgattcatta gcagatgcta
    22981 ttggacgcgt tcaagcaatg acaacgatgg taaaagcaaa caacgacttg atggaacaac
    23041 aaaaacaaga taaaaaagct gtagaagaca aaaaagctga aaacgatgct aaactaaaag
    23101 aattagctga aaaccaagct gctttggaat ctcaaaaagg cgatctttta tctaaacaag
    23161 ctgatttgaa cgtactgaaa acttcattag ctgctgaaca agcaactgct gaagacaaga
    23221 aagctgactt gaaccgtcaa aaagcagaag ctgaagcaga gcaagctcgt attcgtgaac
    23281 aacaacgtct agcagaacaa gctcgtcaac aagctgcaca agaaaaagct gaaaaagaag
    23341 cacgcgaaca agcagaggct gaagcacaag ctactcaagc ttcatctact gcacaaagct
    23401 cagcaacaga agaatcatca gctacacaat cttcaatgac tgaagaatca tcatcagcta
    23461 cacaatcttc agcaacagaa gaatcaacaa cacctgaatc ttcaacagaa gaatcaacag
    23521 cgcctgaatc ttcagcaaca gaagaatcaa caacagcgcc tgaatcttca gcaacagaag
    23581 aatcaacaac agtgcctgaa tcttcagcaa cagaagaatc aacaacagtg cctgaatctt
    23641 caacaacaga agaatcaaca acacctgcgc ctacaacacc atcaacagat caaagtgttg
    23701 atacaggaaa cggcacagga agtagtactc cggctccaac gccaacacca acacctgaac
    23761 aaccaaaacc tgtaacacct gctccagctc cttctggaag tgtgaatggt gcagcaatcg
    23821 tagcagaagc ttacaaatat attggtactc catatgtttg gggcggtaaa gatccaagtg
    23881 gctttgactg ctcagggttc acacgctatg tttacatgca agtaactggt cgtgacatcg
    23941 gtggttggac agttcctcaa gaatcagctg gaacaaaaat ttcagtttct caagcaaaag
    24001 ctggagactt gttattctgg gggtcacaag gcggaactta ccacgtagcg attgctttag
    24061 gcggaggaca atatatccat gctcctcaac caggcgaaag cgtaaaagtt ggttcagttc
    24121 aatggtttgc ccctgacttt gctgtcagca tgtaagaatg attaatcaaa aagagttagc
    24181 ccattctgga ctaactcttt ttttattgtg ataaaataga aatcagataa attcttctgt
    24241 caattccatg aacgtttgga tatcttcagt caccatgtcg atacctgctt gccaaaaatc
    24301 tggttgggta agatcgacat tcaaatgttt cttcgcaaga tcttccgtcg tcattgaggc
    24361 agtatcgcgt agtaaatgga tatattcttc ttcaaaattt gctccctttt ggttggcgta
    24421 agcatagatg cccaaactaa agaggtagcc aaatgtgtat gggaagttat aaaacggtac
    24481 gtcatcaata aagaaatgca attttgcagc ccagaaatat ggatggtaag tagctaaagc
    24541 tccttgatag ccttcttttt gcgcctcaac catcatctct gtgatcttat cttctgagac
    24601 taatccttct tgccgtgctt catagaatcg attttcaaaa atgaatcggg aatggatatt
    24661 catgaacata gctaaagcat tttgcagttt tgtatctagc aaattgattt tttcttcttt
    24721 tgtttttgcg acttttaatg tagcatcggc tacgatcagt tctgcgaatg tacttgctgt
    24781 ttctgcgaca ttcatggcat attcgcgatt taaagaaggt aaatcccaca tcgtgctgct
    24841 atggaaagca tgtcctagtt catgagcaag tgtcgcaacc tcattaacag aattactgta
    24901 ggtcataaag atccttgatt cttttgtttc tggtaactca gtacaataac caccaggacg
    24961 tttcccagga cgatcttctg cttcgatcca gctcttttca aaagcagatt gggcaaattt
    25021 agccattttt ggactgaatt tgttaaagtt ttcgataata aatgctgctg cttcgtcaaa
    25081 actaaaggtt ttttctttca aatcgcctaa aataataggt gcatcttgat cttgccattc
    25141 cattttttct ttaccgaaaa gattagcttt tctagtcaag taattaacaa ggggttgttt
    25201 gtttttctgg attgtatccc acatgacaga caatgtttct ttcttcaacc gattgtactc
    25261 taatggtttt tgtagaaaat ctgtgacacc atgaagttca tagtcagaaa gtctaaaacc
    25321 atctaaatga ttcaaagtat ccgtgaagag gggtgccttt tctttccagg cttcttccca
    25381 tgctgcgaaa agtgtttctc tgacttttgg atctggatcg cccatcatct tattgaaggc
    25441 ttgtccagca gataattcga ctacttgtcc atcttgttcg aagggaatcg aaatgctggc
    25501 tacaatcgta tcataatgac tgctccaagc atttagacca tctaaagaaa gcgtatttat
    25561 aatgttttct tcagcttctg ataataattg tgagccatca cgacgaatct cgtttaaacg
    25621 aaaagcaatt gtttcaaacg aagattgaga aagcatctga ttccaatcat tgtcagatat
    25681 ttctgcaaat ttctttgaca atacagtttc ggctgattgc agacgaggca gcattgcgta
    25741 aagcttgcct gataagacct ttgcatctga atcattgaca ttggcagaaa gcaatgcagt
    25801 aatatagctg ctgcattggg taaaaccatt cgtaatcgtt tcttgcaatt gaagaatagc
    25861 gtttaactgc tctgcattgt cagaactagg tgaccatttt gttacgcggt gataatactc
    25921 gttcatttgg tcttccaatt gcttcatccg ttggttcaaa gcatcagaat gactgcctcc
    25981 ggaaaaaata gaatctagat tccaatttaa tgaataactc atgaaaaaac tcccttcata
    26041 ttgtaataca aaattaagta tagcactttt ttatggaaga agtctctatg aaaatgattg
    26101 ttgtttcttc aaaaaaagtc taagataaaa tacagaggtg acttatgaat tcttcaaaaa
    26161 aacaaatgaa aaacggaaat atttatttag taatcgcact gatagtcgtt ataatcttat
    26221 tttacagttc ttctcaaact tacggacagc aatcacaagt ttcgcgatta gaaagctggc
    26281 tgcctaatca gccatttaaa gacacattag cgggtatacg ttttaattat ggtggaagta
    26341 tggtgagtat caaccatctg ggctacgcca aatttttaga gttcttttta aggaagggcg
    26401 cacatttcgg tacctatttt attttgggag gcagcttgtt tttaggtgtt ttccccaagc
    26461 taaaaatttg gtggctgaca gcgatccttg cttggctttc agcaacaggg tacgcaggtt
    26521 tggacgaatt ccatcagatg atgaccggtg gacgaacacc tatgttccaa gatgtcatgt
    26581 tggattcgat cggtgctctg actgctgtga tattatgtgt gctttttgcg gcattcaaga
    26641 agcgtttata gattggctgt ggctttgttt ttttgtgaaa tagtactaga atagaagtgt
    26701 attctcaaaa gaacaaggga ggaaaaatca tgtcaggaca tagtaaatgg cataatatcc
    26761 aaggaagaaa aaatgcccaa gatgcaaaac gagggaaagt ctttcagaaa ctttctcgtg
    26821 aaatttatat ggcagctaaa gcaggcggtc ctgatccatc tatgaatcca gctttgcggc
    26881 ttgttgtgga caaagcaaaa gctgcaaata tgcctaacga caatgtggaa cgggcgatta
    26941 aaaaggctac gagtgccgta gatgaaacga attacgatga aatcacgtat gaaggatatg
    27001 gtccgggtgg agtaggaatc ttagtccatg cattgacaga taaccgtaat cggacagcga
    27061 caaatgtccg agtagctttt actcgaaacg gtggatcact gggagaaacg ggttcagtca
    27121 gttacttgtt tgaccgcaaa ggatatatcg ctattgaacg cgatgggttg actgttgatg
    27181 aagagacgat gtttgaggat gtattagagg cgggggcaga agatctgcaa acttctcctg
    27241 aagtattcga gatttatact gcaccagaag attttgctgc tgtacgagat gagttggaaa
    27301 aagagttcac tttggcgcaa gcagaattga cgatgattcc acaaacgacg attgatttaa
    27361 atgaggaaca aaaagagcaa ttggaacgtc tagtagataa gctagaagac gatgatgatg
    27421 tttcagaagt ctttactgct gctgaaatgt aagtagcaca ttgttagata ataaaaagaa
    27481 agcacctgta ccaatcgtac gggtgctttc tttttatata ttatttttgt tagaagacca
    27541 atacaggcgt tcctttttcg accatcccga ataattcctt cattacactt ggtggggtgt
    27601 tgatgcagcc gtgtgaaccg cgggttttcc ataagtctcc accgtattca ggttgccagt
    27661 ctgaatcgtg gataccaaca cctgtccagt caatcggcat ccagtagttt accggactct
    27721 cgtaaggtgt tccatcgtca tttgttcctt ttaaagttgc atcttcttct ttattccaaa
    27781 catagaagac tccagcaggt gtaggtgtag ttggctttcc ggaaacgata tctgtttcaa
    27841 gggccacttt tccgtctttg taataccaca tatgctggtt ctccagatca acttcgatat
    27901 acgtatcttc gatcaatgga tgatcagcgg ttgtgccacc ttgcacaatg ggtgaacgag
    27961 taaaatcttg gcctgccaga atcgcttttt tcaatgcttc tgtctcactg tctgtttgga
    28021 tcgtccaact gtaagtgcca actgggaccg taacttctcc tctttttgta cttttaaatt
    28081 ttgtgtcatt cgtacttgtg ttatactttg tgcctaaatc tgttacatat tgacgtactt
    28141 gttctgtatc taaatcgact tttccatcat tgtaggtcag ccaactcata atatcacttg
    28201 atgggatctg gaaagtctca ccgtttatac tgtaagtagc ttttacattt gcgatagcat
    28261 tcatggatgc gagttgttct ttcagtgagc tgtcttcaga tgttactttt ggtttttctt
    28321 tgaattcagt caattcaatt gtatctttac cagagtttac cgcacttttt aattgtttga
    28381 ccgcagcatc gacgtcgatc gtatttccat tgacttcagg cttgataacg aaagagtcac
    28441 ctgctttttc gatcgttgca tcagtagtag gtgtccggtc tttgttcaaa tcagtcagct
    28501 tagttgtcaa tgtctgaacg gtagtgtcta gtttttggtg gttttgactg acaggatcta
    28561 tttcttgttt ttctgccgct gagacataag tcattcccca agtccatccg ttttgttcat
    28621 ttaggatcgg agaaagctcc gaagtgaaat catctttgta gccgagctcg gattttggta
    28681 cttcctgcca gacttttcca tcttctttaa tagccagttt tttatcagaa taagcttctt
    28741 ttaatttttc atttgctttt tcgactgtta gattgctgac atttattcca tttatttctg
    28801 tattcggcaa aaaatgcttt gtgtagtatg tactgcgata ggtataaaag ctgatcagca
    28861 ctaagagaac accaagaact gaaagcaaag caacatgttt cgattttttt cgatgcgaag
    28921 atcttgtcat atcgacaact ccttaagtaa aagtttaggg tggaacggtc tcatgatagc
    28981 ataatttaga ggaaaagacg aaggttatct ctactttatg aaaaaattta gaaaccataa
    29041 gggtgataaa ataaaaaaac ggaaaacacc gaatagatgt tttccgtcat gttcaaagat
    29101 tattcagcgt ctagtttagc aaaagcttca tctagaattt cttttaattg cttagcagaa
    29161 gcagccatac ggtcttgctc gctgtcactt aaaggaattt cgattacttt ttggatacct
    29221 tgacggttga taacagcagg tgcaccaatg tagatgtcgt tcaatccata ttcgccattc
    29281 atgaatactg ataaaggaag aacagcattt tcatcatcta agatggcacg agtgatacgt
    29341 gctaatgcta ctgcgatacc atagaaagta gcgccttttt tctcgataat tgtgtaagca
    29401 gcgtcacgta cgccaaagaa taggttaacc atagcttctt catcgatttc tgggttgttt
    29461 tttacccatt catagatttg caagccggca acatttgcat gtgaccaaac tgggaattct
    29521 gaatctccgt gttcacctaa gatgtaagca tggacgttac gtgcatcaac gtcaactaat
    29581 tccgcaagtg cttgacggaa acgagctgag tcaagagagg tacctgaacc gattacgcgt
    29641 tctttaggga aacctgagaa tttccaagtt gagtaagtca aaatatctac tgggttagcg
    29701 gcaactaaaa agataccgtt aaatcctgat tcaacgattt gtgtgacaac atcgcggttg
    29761 atttttaggt ttttgtttac taagtctaaa cgagtttcac ctggtttttg tggtgcgcca
    29821 gctgtaataa caactaagtc agcatcatgt gcgtctgcgt aagtagcaga atagattttt
    29881 ttaggtgaag taaatgccaa agcgtgagac aagtcaattg cgtctccttc agttttagca
    29941 gtattgatat ccacaatacc tacttcttga gcgatatttt gagtaactag agcaaaggca
    30001 tagctagaac ctacggcgcc gtctccaaca agaataactt tttgatgatc ttttttttct
    30061 gcagctgcag tcatttagta tcaatccttc cattgatttg ttttttccca tgcttatgct
    30121 aacatttctc aagccccatg tcacgctatt tggctataaa atataacaga taacaaggtt
    30181 gtattatatg taaaagctta catgtgcgtt atttcactat ttttatatat caattatttc
    30241 attaaacttt gaagaaattt ttaatttctt tatcatttat taacgattct cttttagccg
    30301 aaacgccttg ttttgtgtta tactatgcat tgacaagtgt aaaaaagaaa aaagctcgat
    30361 ggactggacc gctaattagc agtccagcct tttgtgctgc tttttagaat taggtggaaa
    30421 acaaaaatga aaatgatcgt cgggttagga aacccaggta ccaaatacca atatacaaaa
    30481 cacaatatcg gctttatggt cgtggataaa attgcacgcg agcatcaagc tactttcaaa
    30541 aaaaatccgt tcgaagcaga agttgccgaa ttttttcata atggagaaaa gatcctttta
    30601 gtcaaaccac aaacattcat gaatgaatct ggtcgtgcag tcggcccact aatgacttat
    30661 tttgggattt atccagagga attagtcgta atctacgatg atttggatct ggcagtggga
    30721 aaaatccggc tccgtcaaaa gggaagtgcc ggcgggcata acggtatcaa aagtattatt
    30781 tcccatttga atacgaatgt ctttgatcga atcaaggtgg gaatcggcag gccagaaggg
    30841 aaaaaaacag tagtccagca tgtgcttagt cctttcagca aagaaaatca gcctttgata
    30901 gaagaaagca tgtgtcaatc tgttaaagca gtcgaatatc tcatagaagg gcattcgttc
    30961 gtggatgcga tgaatcgatt caattgagga aggaggaaaa ggaatggata ttattcaatt
    31021 agtcggaaaa gatccgcaag taaaagaatg ggtcagcaat ttagaagaaa aaatgcctag
    31081 acaactaatc acaggattag cgggctcagc gaaaacactt ctatttgctc gagcgtttaa
    31141 gaaaacaaat aaaaacatga ttattctgat gccaaatttg tattatgcga atcagttagc
    31201 agaagatctg caacatgtga ttccgcaaga tcagatccat ttatttccag tggatgaagt
    31261 gctttctgct gaaatggcct tttcttctcc agaagcgcga ggcgagagag ttgcaacgct
    31321 taacgcactt caagaaaaaa aagcaggtat ctatttgttg ccagtcgcat ctttgcacaa
    31381 acgactgccg aataagaaaa cttggctaca ggcgcaattg aattggcata ttggtgatga
    31441 gatagacgtg gaaaatcttc ctcgccaatt gactttgatg ggctatgtac gagaagagat
    31501 ggtcgcaaaa ccgggagaat tcagtattcg aggaagcatt atcgatattt acccgttaac
    31561 gacaacctat cctgttcgtg tagaactatt tgatgtagag atagattcta tgcggtattt
    31621 tgaagcagaa acacaacgct caatcgaaaa aacggaagaa atctgggttt ccccaacgag
    31681 tgaacaagtc tacggaccag aggatctaga acatggaata gagcaaatgc aaaaaatgct
    31741 agaaaaacgg cttgctgcga ccacagaaga agcagaccgc gagtttttgc aagattactt
    31801 cggtcagctg acttcaagct ggcaagcagg tgtcccgaca gaagaagcaa aattctactc
    31861 agatcttctt taccaagaat ctgtgacgat cttggattat ttcccttcaa atagtttact
    31921 agtcgttgat gattatcagc gaatcatgga aacaaatcgg gaaatcgaaa gggaagcggc
    31981 agaatggcag actcaaaaaa tcagcgaatt gcgggtgttt tctgagcaga cattttcagc
    32041 agatatacaa aaaatcgtgc agaaggaaaa gttcaccaca acctttttct cactcttcca
    32101 aaaagggatg ggaaatcttc gtttccaagc aatccataat tttcaatatc ggacgatgca
    32161 acagttcttc ggacaaatgc cattgctgaa aacagaaatg gaccgttggc gcaagcaaaa
    32221 acagactgtt gtgatcttta ttccgacaaa ggacagaata cgcaaagcag aacaaatgct
    32281 tcgagatgaa gacatattag tagtagaaac agaaaaaaat gcattgatca gaggacaggt
    32341 acaattagtt gaaggagctt tgcaggctgg ttttgaactt cctcaagaaa aaatagtagc
    32401 tatcacagaa aaagaaattt tccaaaaaac gacgaaaaaa caaacgagac gtcaaacagt
    32461 aacaaacgca gagcgattga aaagctataa tgagttaaaa gcaggagatt acgttgtcca
    32521 tgccaatcat ggtatcggga aatacattgg gatggaaacg ctagaagtag atggcgttca
    32581 ccaagactat atgactattt tgtatcaaaa cgacgacaaa ctgtttattc ctgttactca
    32641 actgaatctg atccaaaaat atgtcgcctc tgaagccaaa gcgcctcgca tcaataagct
    32701 aggcggaagt gaatggacga aaaccaagcg aaaagtctct tcaaaaatcg aggatatcgc
    32761 tgacgatcta atcaaattat atgccgcaag agagtcagaa aaaggatatg cttttggacc
    32821 tgatgacgcc taccaaaaag aattcgaaaa tgccttcccc tactctgaaa ctgatgacca
    32881 gttgagaagt gcagcggaga tcaaacggga tatggaaaaa gaaaaaccaa tggatcgact
    32941 actcgtaggt gatgttggtt atgggaaaac agaagtagcg cttcgagcgg cctttaaagc
    33001 gataaaggaa agcaaacaag tagccttttt agttccaaca acgatcttgg cacagcagca
    33061 ctatgaaacg atgctggaaa gatttgaagg tttccccgta aatatcgggc tattaagtcg
    33121 tttcagaaca agaaagcaac aaaaagaaac catcgaacaa ctgcgtacag gtcaagttga
    33181 tattgttgta ggaacccacc gaattttgtc aaaagatatt gaatttagcg atttgggtct
    33241 actgatcatt gacgaagaac aacgttttgg tgtaaaacat aaggaacgat tgaaacagtt
    33301 acgtgcacaa gtggatgttt tgactctgac tgcaacaccg attccgagaa ctttgcatat
    33361 gtcgatgtta ggcgtgcggg atctttcagt aatcgaaaca ccgcctgcga atcgttatcc
    33421 gatccaaacg tatgttatgg aaaaaaatcc tggggctatt cgcgaagcaa tccatcgaga
    33481 aatgggacga ggtggacaag tattctattt atataatcgg gtagaaacga tcgaacaaaa
    33541 agtagaagaa atccaagaac tcgttccgga agcacggatc ggttatgctc atgggcaaat
    33601 gacagaggcg caattagaaa atacgctttt tgaatttatt gaaggtcaat atgatgttct
    33661 tgtcactaca accatcattg aaacaggagt agatatcccg aacgcaaata cgttgttcgt
    33721 tgagaacgct gattatatgg gattatccac gttataccaa ttgcgagggc gggtaggaag
    33781 aagcagccgt gtcgcatatg cttactttat gtatgagcag cagaagatcc ttaacgaagt
    33841 cagtgaaaaa cgtcttcagg caatcaaaga ttttacagaa ctgggatctg gattcaagat
    33901 tgcgatgcgg gatctttcca ttcgcggtgc aggtaatctc ttaggtgctc agcagcatgg
    33961 atttattgat gcagtcggtt tcgatatgta ttctcaaatg ctaagcgaag ctgtttctcg
    34021 taaacaagga aagaatagtc aagtggagaa gaccactgtt gagatcgacc tcggtgtcga
    34081 tgcgtatcta cctgaaacat acgtggcaga tcaacggcag aaaatcgaga tctataaacg
    34141 aattcgtgaa cttgattcgc aagaaatgct agatgaacta gaagatgatc tgctcgaccg
    34201 ttttggagaa tatccagaag aagtagcaca tctcttgaca attgggcaga tcaaaatgga
    34261 tggcgatcga gcgctcctag aaacgataag gaaacaggag gcgcatatag tctttactct
    34321 aagtaaagtt ggaacaaaac gctatagtgt ggaacagtta ttcgaagcac tgacagcgac
    34381 aaaactaaaa gccagtctag gcgttgaaaa agaacagatg gtgatacgct tgacgatccc
    34441 caaccaaatg gaagaagcag tatggcttca ggaaatccaa aagtttgtca aagcgttgcg
    34501 tgagcaaaaa tatctgtcag cgtaggaagg agcaatgatt tggatcataa agaaatgcgg
    34561 cgcgtcatgc aaggagcatt tgtattgacg attgcttctt tcatagcaaa agtattaagt
    34621 gccctttacc gaattcccct ccaaaactta gtcggcgacg aaggttttta tgtttatcag
    34681 caagtttatc caatttatgg gatagcaatg actttagctt tatccggtct tccgcagttc
    34741 atatcaaagt acgttgcaga aagaaagaat cctttcgagc gaaagcaagc tttgcaaaat
    34801 ctttatccct tagtattttg gacaggtgtc tttttgtggt tgtttgtctt tgcatttagc
    34861 agctggattg cttggatgat gggagacata caattgaaac cgctgatcca agtagtttcc
    34921 tttacctttt tgctgatgcc tggtctatct ttttatcgag ggaatttcca aggaagattc
    34981 ttgatggaac caactgctgt ttctcaagta gttgagcagt tcgttcgtgt ttttgtgatt
    35041 gtctgtagtg cgtttgcttt tagaaagctt ggatggacgg tgtaccaaac agggacggca
    35101 gcaatgagtg gagctgtttt tggcggttta tgcgcatatg ggatccttca ctactatgaa
    35161 caaaagattc atggaggagc gttaagcttc cgacggtttc cattgttcaa acagccgtca
    35221 aagcccttga tccggcgttt tctggtagaa ggcggactgg tttcgattta tagtggattg
    35281 cttatctttt tccagctgat cgactctttc tttgtaaaaa atgctttgga agtttacgga
    35341 ctgtcagaat acggtgcgaa gatagcaaaa ggggtctatg atcgaggtca gcctttagta
    35401 caattaggtt tagtgattgc cactgcatta agtgcgacat tcctgcctgc gttgactcgt
    35461 catttgacca accggatcta tcgtcagttt ttacagacag ccaaaatcta tcttagattg
    35521 acgaccgctt tagcattagc tgcgtctttg ggattagcct tgctgctccc ttatatcaat
    35581 tatgcgttgt ttaaagatta tgcgggaaat gcagcgcttg tattatttgt tttttccatt
    35641 gctttcactg cagtcatcca agcttatcaa agtattgctc aaagcaagaa ctcgtttcgc
    35701 ccttctttaa aaggcgcagg atggggacta ttggtaaaag gattgactac ttcttttctg
    35761 accggtttat taggaacagc aggtgcaagc ctgtctaccc ttttaggatt aggtgtgacg
    35821 ctttggtatt ttgttcgaat tgaaacaaaa gagctaaatg ctttttggaa agaacggcat
    35881 tttggacgga agttgatgtt cagccttctt tgtatgacag tagtactgct gctgtattat
    35941 ggtgtattga atatagtatt tggtcctgta actcatcgtt ccactgcttt ttttgtcagc
    36001 gtgataggag ttgggatcgg ggcttctgtg ttcatttatg cagtgattcg tcttcgtctg
    36061 tttacagtac gagaatggct aatgttgcct tttggtaaga aaatcctgcg attaaataaa
    36121 aaaattaa
//
LOCUS       DAZKAS010000013        34491 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000013 DAZKAS010000000
VERSION     DAZKAS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.013"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8H7_000530"
     CDS             212..604
                     /locus_tag="ACG8H7_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8982657.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8H7_000531"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8H7_000531"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFP8982658.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8H7_000532"
     CDS             2698..2943
                     /locus_tag="ACG8H7_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982659.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8H7_000533"
     CDS             3165..4775
                     /locus_tag="ACG8H7_000533"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFP8982660.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8H7_000534"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8H7_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982661.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8H7_000535"
     CDS             5067..5936
                     /locus_tag="ACG8H7_000535"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP8982662.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8H7_000536"
     CDS             6101..7372
                     /locus_tag="ACG8H7_000536"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP8982663.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8H7_000537"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8H7_000537"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP8982664.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8H7_000538"
     CDS             8763..9026
                     /locus_tag="ACG8H7_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP8982665.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8H7_000539"
     CDS             9471..9920
                     /locus_tag="ACG8H7_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8982666.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8H7_000540"
     CDS             9977..11395
                     /locus_tag="ACG8H7_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP8982667.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8H7_000541"
     CDS             11395..12255
                     /locus_tag="ACG8H7_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP8982668.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8H7_000542"
     CDS             12429..13202
                     /locus_tag="ACG8H7_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP8982669.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8H7_000543"
     CDS             13349..13714
                     /locus_tag="ACG8H7_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFP8982670.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8H7_000544"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8H7_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8982671.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8H7_000545"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8H7_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982672.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8H7_000546"
     CDS             15560..16216
                     /locus_tag="ACG8H7_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP8982673.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8H7_000547"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8H7_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP8982674.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8H7_000548"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8H7_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFP8982675.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8H7_000549"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8H7_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP8982676.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8H7_000550"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8H7_000550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP8982677.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8H7_000551"
     CDS             21888..22202
                     /locus_tag="ACG8H7_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP8982678.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8H7_000552"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8H7_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP8982679.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8H7_000553"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8H7_000553"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP8982680.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8H7_000554"
     CDS             23546..23875
                     /locus_tag="ACG8H7_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFP8982681.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8H7_000555"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8H7_000555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP8982682.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8H7_000556"
     CDS             24863..25687
                     /locus_tag="ACG8H7_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFP8982683.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8H7_000557"
     CDS             25680..26027
                     /locus_tag="ACG8H7_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFP8982684.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8H7_000558"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8H7_000558"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP8982685.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8H7_000559"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8H7_000559"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP8982686.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8H7_000560"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8H7_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFP8982687.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8H7_000561"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8H7_000561"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP8982688.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8H7_000562"
     CDS             31684..32451
                     /locus_tag="ACG8H7_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982689.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8H7_000563"
     CDS             32441..34483
                     /locus_tag="ACG8H7_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982690.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11083 a         6119 c         7246 g        10043 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg a
//
LOCUS       DAZKAS010000014        34485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000014 DAZKAS010000000
VERSION     DAZKAS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.014"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..1462)
                     /locus_tag="ACG8H7_000564"
     CDS             complement(176..1462)
                     /locus_tag="ACG8H7_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP8982691.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="ACG8H7_000565"
     CDS             complement(1455..2723)
                     /locus_tag="ACG8H7_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP8982692.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="ACG8H7_000566"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="ACG8H7_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP8982693.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3496..3942)
                     /locus_tag="ACG8H7_000567"
     CDS             complement(3496..3942)
                     /locus_tag="ACG8H7_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8982694.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4035..4280)
                     /locus_tag="ACG8H7_000568"
     CDS             complement(4035..4280)
                     /locus_tag="ACG8H7_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFP8982695.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4395..4973)
                     /locus_tag="ACG8H7_000569"
     CDS             complement(4395..4973)
                     /locus_tag="ACG8H7_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP8982696.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5050..5580)
                     /locus_tag="ACG8H7_000570"
     CDS             complement(5050..5580)
                     /locus_tag="ACG8H7_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFP8982697.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5584..6444)
                     /gene="rlmB"
                     /locus_tag="ACG8H7_000571"
     CDS             complement(5584..6444)
                     /gene="rlmB"
                     /locus_tag="ACG8H7_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP8982698.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6437..6832)
                     /locus_tag="ACG8H7_000572"
     CDS             complement(6437..6832)
                     /locus_tag="ACG8H7_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFP8982699.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6829..8241)
                     /gene="cysS"
                     /locus_tag="ACG8H7_000573"
     CDS             complement(6829..8241)
                     /gene="cysS"
                     /locus_tag="ACG8H7_000573"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP8982700.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8238..8774)
                     /gene="epsC"
                     /locus_tag="ACG8H7_000574"
     CDS             complement(8238..8774)
                     /gene="epsC"
                     /locus_tag="ACG8H7_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFP8982701.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8860..9099)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9096..10556)
                     /gene="gltX"
                     /locus_tag="ACG8H7_000575"
     CDS             complement(9096..10556)
                     /gene="gltX"
                     /locus_tag="ACG8H7_000575"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP8982702.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10726..11883)
                     /locus_tag="ACG8H7_000576"
     CDS             complement(10726..11883)
                     /locus_tag="ACG8H7_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFP8982703.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11917..13287)
                     /gene="radA"
                     /locus_tag="ACG8H7_000577"
     CDS             complement(11917..13287)
                     /gene="radA"
                     /locus_tag="ACG8H7_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP8982704.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13350..13877)
                     /locus_tag="ACG8H7_000578"
     CDS             complement(13350..13877)
                     /locus_tag="ACG8H7_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP8982705.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14107..14913
                     /gene="proB"
                     /locus_tag="ACG8H7_000579"
     CDS             14107..14913
                     /gene="proB"
                     /locus_tag="ACG8H7_000579"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP8982706.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14910..16157
                     /locus_tag="ACG8H7_000580"
     CDS             14910..16157
                     /locus_tag="ACG8H7_000580"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP8982707.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16233..16577
                     /locus_tag="ACG8H7_000581"
     CDS             16233..16577
                     /locus_tag="ACG8H7_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982708.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16694..17020)
                     /locus_tag="ACG8H7_000582"
     CDS             complement(16694..17020)
                     /locus_tag="ACG8H7_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982709.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17635..18837)
                     /locus_tag="ACG8H7_000583"
     CDS             complement(17635..18837)
                     /locus_tag="ACG8H7_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8982710.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18954..19469
                     /locus_tag="ACG8H7_000584"
     CDS             18954..19469
                     /locus_tag="ACG8H7_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8982711.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19668..20114)
                     /locus_tag="ACG8H7_000585"
     CDS             complement(19668..20114)
                     /locus_tag="ACG8H7_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP8982712.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20161..21639)
                     /gene="msr(C)"
                     /locus_tag="ACG8H7_000586"
     CDS             complement(20161..21639)
                     /gene="msr(C)"
                     /locus_tag="ACG8H7_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP8982713.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21993..22622)
                     /locus_tag="ACG8H7_000587"
     CDS             complement(21993..22622)
                     /locus_tag="ACG8H7_000587"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP8982714.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22606..23070)
                     /locus_tag="ACG8H7_000588"
     CDS             complement(22606..23070)
                     /locus_tag="ACG8H7_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8982715.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23557..24825)
                     /locus_tag="ACG8H7_000589"
     CDS             complement(23557..24825)
                     /locus_tag="ACG8H7_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFP8982716.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25056..25793)
                     /locus_tag="ACG8H7_000590"
     CDS             complement(25056..25793)
                     /locus_tag="ACG8H7_000590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8982717.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25860..26429)
                     /locus_tag="ACG8H7_000591"
     CDS             complement(25860..26429)
                     /locus_tag="ACG8H7_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFP8982718.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26557..27429
                     /locus_tag="ACG8H7_000592"
     CDS             26557..27429
                     /locus_tag="ACG8H7_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8982719.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27771..28643
                     /locus_tag="ACG8H7_000593"
     CDS             27771..28643
                     /locus_tag="ACG8H7_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8982720.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28640..30598
                     /gene="nagE"
                     /locus_tag="ACG8H7_000594"
     CDS             28640..30598
                     /gene="nagE"
                     /locus_tag="ACG8H7_000594"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFP8982721.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30930..31781)
                     /locus_tag="ACG8H7_000595"
     CDS             complement(30930..31781)
                     /locus_tag="ACG8H7_000595"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8982722.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31904..32371
                     /locus_tag="ACG8H7_000596"
     CDS             31904..32371
                     /locus_tag="ACG8H7_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFP8982723.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32634..33116)
                     /locus_tag="ACG8H7_000597"
     CDS             complement(32634..33116)
                     /locus_tag="ACG8H7_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP8982724.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(33216..34079)
                     /locus_tag="ACG8H7_000598"
     CDS             complement(33216..34079)
                     /locus_tag="ACG8H7_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP8982725.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9848 a         7261 c         6215 g        11161 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaaagaaag ccgattttca gtgtcggctt
     3481 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
     3541 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
     3601 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
     3661 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
     3721 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
     3781 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
     3841 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
     3901 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
     3961 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
     4021 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
     4081 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
     4141 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
     4201 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
     4261 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
     4321 aattttcaag tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt
     4381 aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt
     4441 actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct
     4501 acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg
     4561 atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc
     4621 aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact
     4681 tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga
     4741 ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt
     4801 ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt
     4861 cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca
     4921 gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa
     4981 ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag
     5041 aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa
     5101 ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc
     5161 ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg
     5221 taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac
     5281 ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc
     5341 gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg
     5401 aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga
     5461 taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac
     5521 aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat
     5581 ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact
     5641 ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt
     5701 tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt
     5761 ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt
     5821 ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc
     5881 gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg
     5941 tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg
     6001 tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg
     6061 ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg
     6121 atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac
     6181 agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg
     6241 aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa
     6301 gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt
     6361 ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg
     6421 tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca
     6481 attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc
     6541 ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat
     6601 ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt
     6661 gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct
     6721 tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg
     6781 catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct
     6841 tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc
     6901 acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat
     6961 caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga
     7021 aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg
     7081 gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc
     7141 attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat
     7201 tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc
     7261 aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta
     7321 gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac
     7381 gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc
     7441 gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt
     7501 tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc
     7561 accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg
     7621 ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg
     7681 tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg
     7741 acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa
     7801 tttacgggta cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa
     7861 cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc
     7921 aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac
     7981 ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt
     8041 gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc
     8101 gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca
     8161 gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa
     8221 tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact
     8281 tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct
     8341 gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag
     8401 agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct
     8461 aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg
     8521 attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca
     8581 acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc
     8641 tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa
     8701 acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg
     8761 gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc
     8821 ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg
     8881 gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct
     8941 tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact
     9001 cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta
     9061 atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt
     9121 gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt
     9181 tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct
     9241 ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct
     9301 agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt
     9361 gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct
     9421 gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc
     9481 ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca
     9541 gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc
     9601 gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct
     9661 tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg
     9721 aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat
     9781 tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg
     9841 aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca
     9901 atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca
     9961 gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta
    10021 tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga
    10081 ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca
    10141 ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct
    10201 tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca
    10261 agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg
    10321 tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc
    10381 aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata
    10441 ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt
    10501 ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc
    10561 ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct
    10621 caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta
    10681 aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt
    10741 agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc
    10801 aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc
    10861 attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac
    10921 tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat
    10981 gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa
    11041 ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc
    11101 caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc
    11161 gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg
    11221 cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt
    11281 agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc
    11341 aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc
    11401 taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc
    11461 aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa
    11521 gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag
    11581 aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag
    11641 aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat
    11701 atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt
    11761 aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa
    11821 aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg
    11881 catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt
    11941 ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa
    12001 gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg
    12061 ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc
    12121 cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc
    12181 aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg
    12241 aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc
    12301 aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat
    12361 ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc
    12421 gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc
    12481 atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa
    12541 aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc
    12601 taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc
    12661 tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac
    12721 acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac
    12781 ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta
    12841 tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc
    12901 gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac
    12961 ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc
    13021 tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg
    13081 ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact
    13141 tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt
    13201 tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac
    13261 aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca
    13321 ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga
    13381 cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg
    13441 atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg
    13501 atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt
    13561 gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca
    13621 tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca
    13681 ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct
    13741 gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca
    13801 tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca
    13861 aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc
    13921 gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac
    13981 cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg
    14041 actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg
    14101 tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt
    14161 acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc
    14221 ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc
    14281 gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct
    14341 gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat
    14401 aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt
    14461 aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac
    14521 gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat
    14581 cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat
    14641 atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat
    14701 atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga
    14761 acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca
    14821 atgattttag caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa
    14881 gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg
    14941 caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg
    15001 ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag
    15061 acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga
    15121 cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc
    15181 ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc
    15241 gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg
    15301 cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat
    15361 tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat
    15421 ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc
    15481 gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag
    15541 tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata
    15601 tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc
    15661 gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt
    15721 tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc
    15781 gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat
    15841 atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata
    15901 tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc
    15961 aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta
    16021 cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg
    16081 cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg
    16141 gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg
    16201 taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta
    16261 tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct
    16321 ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca
    16381 aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct
    16441 tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc
    16501 tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga
    16561 atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa
    16621 aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat
    16681 aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa
    16741 atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg
    16801 ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca
    16861 taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca
    16921 attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa
    16981 cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt
    17041 ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa
    17101 aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct
    17161 gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat
    17221 tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa
    17281 tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt
    17341 ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca
    17401 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
    17461 ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc
    17521 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg
    17581 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga tttcttattc
    17641 ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac
    17701 aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc
    17761 aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac
    17821 gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc
    17881 ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc
    17941 tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt
    18001 cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc
    18061 ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc
    18121 atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg
    18181 aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt
    18241 ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac
    18301 cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac
    18361 agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac
    18421 taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca
    18481 gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa
    18541 tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa
    18601 gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt
    18661 cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag
    18721 acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc
    18781 ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc
    18841 tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg
    18901 tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata
    18961 ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga
    19021 agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca
    19081 agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga
    19141 tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc
    19201 ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata
    19261 aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa
    19321 aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg
    19381 ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa
    19441 gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta
    19501 ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg
    19561 aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac
    19621 tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt
    19681 tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa
    19741 gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt
    19801 ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag
    19861 catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca
    19921 atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca
    19981 ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac
    20041 gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt
    20101 ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat
    20161 ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc
    20221 agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat
    20281 ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc
    20341 taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc
    20401 tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt
    20461 cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat
    20521 ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg
    20581 agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga
    20641 accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac
    20701 cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct
    20761 gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt
    20821 tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt
    20881 ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt
    20941 ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt
    21001 ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc
    21061 caattccttt tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc
    21121 ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa
    21181 aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc
    21241 tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat
    21301 caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct
    21361 taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa
    21421 ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc
    21481 accggctatt agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat
    21541 acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct
    21601 gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac tcctatttca
    21661 tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg
    21721 catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata
    21781 gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg
    21841 tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat
    21901 cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa
    21961 gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt
    22021 gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa aataataact
    22081 ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca
    22141 ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct
    22201 aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa
    22261 ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa
    22321 gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc
    22381 attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct
    22441 tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt
    22501 gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca
    22561 tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc
    22621 attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc
    22681 taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc
    22741 tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc
    22801 tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc
    22861 caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact
    22921 gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact
    22981 tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata
    23041 taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc
    23101 aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta
    23161 ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg
    23221 gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat
    23281 agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt
    23341 ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa
    23401 gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct
    23461 gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta
    23521 ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc
    23581 ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg
    23641 atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga
    23701 ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg
    23761 tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc
    23821 gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt
    23881 tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata
    23941 catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac
    24001 attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt
    24061 agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg
    24121 ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg
    24181 atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg
    24241 catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc
    24301 agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga
    24361 tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa
    24421 cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa
    24481 ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt
    24541 gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg
    24601 tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt
    24661 agctgactgc aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt
    24721 ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag
    24781 atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg
    24841 aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa gtcaatgtcc
    24901 atatttaaat aacatttgtt tctttttaat aaatttgctt tttctgcata aaaagacgta
    24961 aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa taactattta
    25021 acaagtaatt ctctctggat attatccgtt atggattata cttcctgtgt cgtcggacgg
    25081 ataatcatgt tgctgacaga catccggtct ggcgtttcta tcgcaaatgc tacagcagaa
    25141 gctatatctt cagatgttaa tccccattct ttttgcgctt catgaagttc aagagcagcc
    25201 tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga gatgatcgta
    25261 gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc acggacagca
    25321 aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc cacagaatca
    25381 gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc ggaaattcca
    25441 tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg gtgaccttca
    25501 actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat tcgaccgaac
    25561 ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac atctgctgtt
    25621 tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg atcttctcgg
    25681 cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt tgcttcaccg
    25741 attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt cattctattc
    25801 tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca tcttactgtt
    25861 caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac gatggtattg
    25921 tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc gccaatcgtt
    25981 acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat gtggattggc
    26041 tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt gatgaggttg
    26101 acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc aacgaacatc
    26161 acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa atctgtataa
    26221 aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt ttctagccgt
    26281 ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg ctcttcgttt
    26341 tccgcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat ctctttattg
    26401 acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct tactttataa
    26461 cggacggctt atgatgtcta atacttataa aacatactta actattcctt ttaactatac
    26521 ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat actgacgtat
    26581 tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct gcatatcacg
    26641 caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt gacacttttc
    26701 actcgaggct cccgagagat acagttgacg gattctggtc gatatcttta ttcaaaagga
    26761 aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga cgcaatcatc
    26821 tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat ttctgatagc
    26881 cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag cgggaatgca
    26941 gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt aatcgaaccg
    27001 acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg gggacttttg
    27061 atacatcgga atcatccttt agcaaagcaa acagtcatcc atccgcagga actgaaaact
    27121 gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga atggctaggt
    27181 ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa tgcttctttg
    27241 atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa tacttatggg
    27301 acggaaaaag tattcatccc actcgatccg ccattacaag cttcactgaa tctcatttgg
    27361 aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct aacggagaaa
    27421 tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa taagttaagc
    27481 aaaagatcca atgattaaat aacataagtt tcttaatttg ttccttcctt ttatttctga
    27541 taattccagt aaattactat aattataaaa aaagaattga caaaacataa ctgtctatat
    27601 aaaattgatt acgttaacaa taattcgttt catgtgacta gatcatacta ggcatgaaaa
    27661 atctctgagg aacatggttt ccctaggagg tttttcgtgt ctatttttgt ttggccgcaa
    27721 tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa gggtgcgcgc atgaaaatca
    27781 aaaagatctt taatcaaaat gcagtattgg tagatgacgg tggacaggaa aaagtagcta
    27841 ttggaaaagg gattggattt gacagaaaaa gaaatgattt gatttttgat cgtgatgtcg
    27901 aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt gcaaaattta cttaatcaga
    27961 ttgatgaacg ctttttattt gcagcagaaa cgatcattga tcatgcggaa actgttttga
    28021 tggaaaagtt gaatgaacat gtattgatcg ctttgaccga ccacattgct ttttctgcgg
    28081 agaacattaa taacggtatc gtcatccgca acaaactttt aagagaaatc gaagtgcttt
    28141 acagtgatga attcagtatt gctcaatggg cagtagagta tctgacaaaa gagcttggga
    28201 ttccatatac ttatgacgaa gcaggatata tcgcaatcca tattcacagc ggacgaagcg
    28261 gtcggaacaa taaccatcgc agtatccgtg aagtgacgat tgtatctgac atcatccatt
    28321 tagtggaatc agaattggaa acagatatcc attcagaacc attttcactg aattattcaa
    28381 gactggtgaa tcatttacga ctgcttctac aacgaaccca tgcgcaacaa tatgctgttt
    28441 tagacacgga aatcgtagaa atggtcaaac gaaaataccc agaaagctat aagatttcta
    28501 aaaaaatccg tgtgttgctg accaaagaat accagctcgc gatcacgaaa gaagaactgg
    28561 gttacttggc catccatatc gagcgtttaa gaagcgcaat cgtacaaaca aatgttaaca
    28621 atcatgagga ggaaaaaaat tgaaagcata tctacaacga atgggacgtt cccttcaact
    28681 tccagttgcg gtcttacctg ctgctgcttt actagtaggg atcggaaact ggtgggcatc
    28741 ttatagtaac gacattgtcg ctcatttttt acaagctggc ggtaacgcag ttttaggtca
    28801 attacctttg ctgtttgccg ttgggttagc actagggatg tcagtggaca aatcaggtgc
    28861 tgctgccttg gcaggtttgg tcgcatttga agttccagta aatgttttaa aaactgattc
    28921 agtagctact ttattgaata tcaaaccaga atcagttgat ccatcttttg ctcagatcag
    28981 taacgtattt attggtatta ttgccggatt gattgcggca gctctttata atcgtttcca
    29041 cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc aagcgtctag tgccaatctt
    29101 ggctgccttt gtcatgttgc tgatcagtgc agtgctactg ctcgtatggc ctccggttta
    29161 ctctgcttta gtttcttttg ctaaattcat tttgagttta ggctggggtg gtgcaggtct
    29221 ttatggcttc ttcaaccgtt tactgatccc tacaggattg catcaagcaa tgaactccgt
    29281 gttctggttt gacgtagcag gaatcaatga tattggtaaa tttttggctt ctgaaggaac
    29341 aaaaggcgta acaggaatgt atcaagctgg atttttccca atcatgatgt tcgggcttcc
    29401 agcaggagca tatgctattt acagaaatgc acgtccagaa aacaaagcaa aaacagcttc
    29461 cttgatgtta gcagcagctt ttgcttcatt ctttactggt gtaacagaac cacttgaatt
    29521 ctcatttatg ttcgttgctt ggccattata tgtcttgcat gcaattttca ctgggatttc
    29581 tttagcagtg agtgcattct tccattggac agcaggattt gcctttagtg ccggatttgt
    29641 cgattacttc ctatctttga aaaatccgat tgcgaatcag ccattgatgt tgatcgtcca
    29701 aggattagta acagcagtga tttactactt tggctttaac tttgctatca aaaaattcca
    29761 tctgatgact cctggccgcg aggaagcaga tctttcagat gacgatactg caacgactaa
    29821 ttcagacaat aaatatgcgg cacaagcaag caaaatctac gctgcattag gcggagccga
    29881 caatgtaact tcgatcgata actgtactac acgccttcgt ttgcaagtca aagatacagg
    29941 cctgatcgat caaaataaaa tcaaagccac tggcgtacct ggtatgaaga tcatcgacgg
    30001 aaaaaatgcg caagtcatcg taggtacaga agtacaattc gttgcagatg agatggcaaa
    30061 actacatggt ggtgctgcaa cacgacctgc acaaacaaac accgtggtta aaacagaaac
    30121 atttgccggt ggaacgatcg aacaagatat ttacgctatc gcaaatggga aactgattcc
    30181 aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg atgggtgacg gctatgctgt
    30241 attaccagaa aatggagaaa tcttctctcc aatcgctggt acgatcacaa acattttccc
    30301 aacgaaacat gctgtcggaa tccaaacaga tgctggtatc gaagtcttgc ttcatatggg
    30361 catcaacact gttgacttaa aaggcgaacc attcacttta tatgtaaaag aaggtcaaaa
    30421 agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc gctattcaat ctgcaggcaa
    30481 aaacacggat atggtcgttg tattcacaaa tggcgacaaa gtccaaagct tagaaatcga
    30541 acccgcacgt gatgtaaaag caaatgataa aatcggctct gtttccaaca aagcctgagc
    30601 catcgtcttc cccagacttt tttatcgata aacaaacagg agctgagaca aaaatcttgt
    30661 ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc aactccgcgg taataagtcc
    30721 agccaaacgt aacatatgaa gagctatttt ttatttgact cttcttatta cttgatgcag
    30781 aacagctttt ttacaacctc tgataaacgg tgttcaaaaa ccagtttatc ggtatgaagt
    30841 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttcat actcaaaact
    30901 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    30961 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    31021 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    31081 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    31141 tttttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    31201 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    31261 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    31321 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    31381 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    31441 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    31501 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    31561 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    31621 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    31681 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    31741 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    31801 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    31861 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    31921 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    31981 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    32041 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    32101 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    32161 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    32221 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    32281 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    32341 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    32401 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    32461 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    32521 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    32581 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    32641 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    32701 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    32761 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgattaca
    32821 acaagatacg ttccttttgg gaatgggatg attcgggtag tgtttggaac atcctgactc
    32881 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    32941 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    33001 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    33061 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    33121 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    33181 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    33241 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    33301 gcttcgacta actcataatc tgaagaagga agccattctg catagatttt cccccaagta
    33361 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    33421 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    33481 tgcatcaaac tgcccttttc ttccaagcgg gctccatcaa atccataaga aacgttgatt
    33541 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    33601 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    33661 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    33721 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    33781 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    33841 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctaggag atcgcgatta
    33901 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    33961 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    34021 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    34081 tttatcttcc tccttgcttg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    34141 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    34201 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    34261 aataaatttt ggggaagaca cattctaagt aaacaaagcg gtaaaaatat cttttattcc
    34321 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    34381 gaacgctgct tggatttttc tttgcgatac atcatacctc ttttatcaat tggaacaatt
    34441 atttatttct aacaaaatca cttcattttt gtcagataaa taaaa
//
LOCUS       DAZKAS010000015        32039 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000015 DAZKAS010000000
VERSION     DAZKAS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.015"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..414
                     /locus_tag="ACG8H7_000599"
     CDS             <1..414
                     /locus_tag="ACG8H7_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFP8982726.1"
                     /translation="LYTDTKTIREYFGIEYSSNLGDVLFQFSQTLARFIPTEVSEKKN
                     EDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKRSPFNDNKTRLYRPSLYE
                     RLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            486..881
                     /locus_tag="ACG8H7_000600"
     CDS             486..881
                     /locus_tag="ACG8H7_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8982727.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            865..3318
                     /locus_tag="ACG8H7_000601"
     CDS             865..3318
                     /locus_tag="ACG8H7_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8982728.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            3315..5342
                     /locus_tag="ACG8H7_000602"
     CDS             3315..5342
                     /locus_tag="ACG8H7_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP8982729.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5339..6361
                     /locus_tag="ACG8H7_000603"
     CDS             5339..6361
                     /locus_tag="ACG8H7_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFP8982730.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            6378..7307
                     /locus_tag="ACG8H7_000604"
     CDS             6378..7307
                     /locus_tag="ACG8H7_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8982731.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            7621..7668
                     /locus_tag="ACG8H7_000605"
                     /pseudo
     CDS             7621..7668
                     /locus_tag="ACG8H7_000605"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7684..8484
                     /locus_tag="ACG8H7_000606"
                     /pseudo
     CDS             7684..8484
                     /locus_tag="ACG8H7_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            8722..8958
                     /locus_tag="ACG8H7_000607"
     CDS             8722..8958
                     /locus_tag="ACG8H7_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982732.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            8965..10917
                     /locus_tag="ACG8H7_000608"
     CDS             8965..10917
                     /locus_tag="ACG8H7_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP8982733.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(10989..11486)
                     /gene="dfrG"
                     /locus_tag="ACG8H7_000609"
     CDS             complement(10989..11486)
                     /gene="dfrG"
                     /locus_tag="ACG8H7_000609"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP8982734.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            11761..12888
                     /locus_tag="ACG8H7_000610"
                     /pseudo
     CDS             11761..12888
                     /locus_tag="ACG8H7_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            12986..13174
                     /locus_tag="ACG8H7_000611"
     CDS             12986..13174
                     /locus_tag="ACG8H7_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP8982735.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(13232..13585)
                     /locus_tag="ACG8H7_000612"
     CDS             complement(13232..13585)
                     /locus_tag="ACG8H7_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982736.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            14116..14538
                     /locus_tag="ACG8H7_000613"
     CDS             14116..14538
                     /locus_tag="ACG8H7_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP8982737.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            14535..14765
                     /locus_tag="ACG8H7_000614"
     CDS             14535..14765
                     /locus_tag="ACG8H7_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982738.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            15263..15463
                     /locus_tag="ACG8H7_000615"
     CDS             15263..15463
                     /locus_tag="ACG8H7_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFP8982739.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            15490..16683
                     /locus_tag="ACG8H7_000616"
     CDS             15490..16683
                     /locus_tag="ACG8H7_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8982740.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(16751..18316)
                     /gene="guaA"
                     /locus_tag="ACG8H7_000617"
     CDS             complement(16751..18316)
                     /gene="guaA"
                     /locus_tag="ACG8H7_000617"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP8982741.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(18375..19298)
                     /gene="coaA"
                     /locus_tag="ACG8H7_000618"
     CDS             complement(18375..19298)
                     /gene="coaA"
                     /locus_tag="ACG8H7_000618"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP8982742.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            19494..20099
                     /locus_tag="ACG8H7_000619"
     CDS             19494..20099
                     /locus_tag="ACG8H7_000619"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8982743.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            20407..21423
                     /locus_tag="ACG8H7_000620"
     CDS             20407..21423
                     /locus_tag="ACG8H7_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8982744.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            21564..22865
                     /locus_tag="ACG8H7_000621"
     CDS             21564..22865
                     /locus_tag="ACG8H7_000621"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFP8982745.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            22911..23573
                     /gene="deoC"
                     /locus_tag="ACG8H7_000622"
     CDS             22911..23573
                     /gene="deoC"
                     /locus_tag="ACG8H7_000622"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP8982746.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            23594..23992
                     /locus_tag="ACG8H7_000623"
     CDS             23594..23992
                     /locus_tag="ACG8H7_000623"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP8982747.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            24058..25140
                     /locus_tag="ACG8H7_000624"
     CDS             24058..25140
                     /locus_tag="ACG8H7_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8982748.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            25352..26914
                     /locus_tag="ACG8H7_000625"
     CDS             25352..26914
                     /locus_tag="ACG8H7_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982749.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            26907..28040
                     /locus_tag="ACG8H7_000626"
     CDS             26907..28040
                     /locus_tag="ACG8H7_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982750.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            28040..28996
                     /locus_tag="ACG8H7_000627"
     CDS             28040..28996
                     /locus_tag="ACG8H7_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982751.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            29095..30261
                     /gene="deoB"
                     /locus_tag="ACG8H7_000628"
     CDS             29095..30261
                     /gene="deoB"
                     /locus_tag="ACG8H7_000628"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP8982752.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            30285..31103
                     /locus_tag="ACG8H7_000629"
     CDS             30285..31103
                     /locus_tag="ACG8H7_000629"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP8982753.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            31120..31827
                     /gene="deoD"
                     /locus_tag="ACG8H7_000630"
     CDS             31120..31827
                     /gene="deoD"
                     /locus_tag="ACG8H7_000630"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP8982754.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        10508 a         5630 c         6617 g         9284 t
ORIGIN      
        1 ttatatacag acacgaaaac aataagagaa tattttggta tcgaatacag tagtaacttg
       61 ggagatgttt tatttcaatt tagccaaact cttgctcgat ttattccgac agaagttagt
      121 gagaagaaaa atgaagacca aaaagaagcc atgtgtttct cattaagtca aagtgattca
      181 gaagatccca gaaaaaaata ttgtttttca gttagaagaa atcctttaaa gggaaatggt
      241 gagctgggaa aacgaagtcc ttttaatgac aataaaacga gattgtatag acccagctta
      301 tatgaacgat tagggtcaga taccaattta agtttcagat attcaatgaa tccagatgat
      361 gaagaaactg atgaaggtat tattgcaaaa tggacaaaaa ataaaattga atagttaaag
      421 cattgaaaga gcaatcacct attttgattg ttctttttct atttttacgg aaaggacgtg
      481 aagacatgaa aaaaataaga agttacacca gtatttggtc agtagaaaaa gtgatttatg
      541 cgattaatga tttacaactc cctttcccaa ttacctttac gcaaatgaca tggtttatcg
      601 tttctttgtt tgtcgtcata ctctttggca gtttaccacc actttctctg attgacggag
      661 cactactcaa atacttaggg attcccattg ttctcactgg gttcatgtgc caaaagacct
      721 ttgacggaaa gaaaccctat ggatttctaa agtctatcat tacctatgct ctacgcccta
      781 aagtaaccta cgctggcaaa caaatacgat taaaaaaaga aacaatgaat gaatcgatta
      841 cgattgtaag gagtgaacaa ctagatggca tatccgatta aatatatgga agataatctc
      901 gtatttaatc atgatggcga agtattcgct tattatgaat taattcccta taattacagt
      961 tttttaagtt ccgatgaaaa aattcaagtt catgataatt tctgtcaatt gattgcccaa
     1021 aatcgtgatg gcaagattca tgctctgcaa ctagcaaccg aatcaagtat tcgagccaca
     1081 caggaacaat cgaaaaaaga aatcactgga cgattaaaag agatagctta cgaaaaaata
     1141 gatgaacaga ccgaagcgtt aatttctatg attggtgaca atcaagtaga ctatcgcttt
     1201 tttattggtt ttaaactcta cctcaatgaa caggaagtat ccgtaaaaaa tgttgggaaa
     1261 gaaattcaaa aagcatttgc ggaattcttt catggtgtga atcacaaatt aatgggcgat
     1321 tttgtttcta tgcctatggc ggaaatcgaa cgcttttcta agatggaaca gttgttagaa
     1381 agtaaattat ccagacgctt tcagattcgt aaattggaga aagatgattt tggttatatt
     1441 attgaacatc tttacgggca aacaggtgtc ccttttgatg actacgacta ccatttaccg
     1501 aaacgatacg aagataaaaa agtgttggta aagaaatacg atattctcaa acccactcgc
     1561 tgtttaatcg aagaaaatca acgctattta cggattgaac aggaagatac cactacttat
     1621 gtggcgtatt ttacaattaa tagtattgtt ggtgaattgg atttccctaa tagtgaaatt
     1681 ttttattacc agcaacagca gtttactttc cccgttgata ccagtatgaa tgttgaaatt
     1741 gtcgccaata aaaaagcctt atctaccgtt cgcaataaaa agaaagaact gaaagattta
     1801 gatagccacg catgggaaag cgacaacgaa accagtacta atgtggtgga cgctttagaa
     1861 tctgtcgatg aattagaaag taatttagac caaaccaaag aatctatgta caaactaagc
     1921 tatgtggtac gggtatcagc aaagaatttg gacgagctga aacaacgttg tgatgaagta
     1981 aaagattttt atgatgatct gtctatcaaa ttagtacgac cttttggcga tatgctaggc
     2041 ttgcataatg aatttattcc ctcaagcaag cgatatatca atgactatat tcaatatgtc
     2101 acgtcggatt ttttggctgg tcttggtttc ggtgctacac aaatgattgg tgaaaattct
     2161 ggtatttacg tcggttacaa tctcgataca ggcagaaata tttatctcca acctagcctt
     2221 gcaagtcaag gaattaaagg ctctgtaaca aatgcattag cgtccgcttt tattggttca
     2281 ttaggtggcg gaaaatcatt ctctaacaat atgctggtct attattcagt actctttggc
     2341 ggacaggcaa ccattgttga cccaaaagct gaacgtggtg aatggaaaga aaccttacct
     2401 gatattgccc atgaaatcaa tattgttaat ctcacgagtg agaacgacaa taaagggtta
     2461 cttgaccctt atgtgatttt aaaactcccg aaagattcag aaagccttgc cattgatatt
     2521 ctcacgttct taacaggtat ttccagccga gatagcgaga agtttcctgt cctgcgtaaa
     2581 gctattcgga gtgtaactca aagcaaacaa cgagggttgt tattggtgat tgatgaattg
     2641 cgaaaagaag atacgcccat cagcaatagt attgctgacc atatcgaaag ctttgtcgat
     2701 tatgattttg cccatctgtt attcagtgat ggcagtgtca cccaatccat tagcttggaa
     2761 aaacaactca atattattca agtggcagat ttggtcttac ccgattccaa tagctctttt
     2821 gaagaataca ctaccatgga attattaagt gtggcaatgc tgattgtcat tagtaccttt
     2881 gctttagact ttatccactc tgaccgttcc atttttaaaa ttgttgacct tgatgaggct
     2941 tggagttttt tacaagtggc acaaggaaaa gctctatcta tgcgattggt tcgagcagga
     3001 cgtgccatga atgcaggggt ctatttcgtt actcaaaacg cggacgattt actagatgaa
     3061 aagatgaaga ataatattgg cttgaaattt gcctttcgtt ccactgacct agtggaaatc
     3121 aaaaagacct tagaattttt tggtgtggac aaggaagatg aaaacaatca gaaacgctta
     3181 cgtgaattag aaaatggaca atgtttagtt tcagacatct atggtcgtgt aggggttatg
     3241 cagtttcacc cgatttttga agagttgctt catgcctttg ataccagacc gcctgtgcga
     3301 aatgaggtgg aagaatgaca caaaaacaaa agaaatttct tcactatagt tggataacgc
     3361 tcctttgtgt gggcgttatt ttacttttat taggcacgct gggcaatgca gtacaagcga
     3421 caggcttagt tgatgaaacc attgatacaa gcaatgaata ctccaagtat ggtttgaatc
     3481 aatatcagct tgattattat gtggacaata gctggggctg gcttccatgg aattggagtg
     3541 atggcatagg acaatcggtg atgtatggat tgtatgccat tactaatttt atttggacaa
     3601 tttctcttta tctatcgaac gccacaggtt atttggtgaa agaagcctat gccttagatt
     3661 tcattagtca aacatcccaa gcaattggcg aaaacatgca gacgattgca ggcattacac
     3721 cgaatggctt ttccagttca gggttttatg tcggtttcct ccttcttttt attctcattg
     3781 ttggaatcta tgttgcctat acaggattgg ttaagcgaga aacgacgaaa gtgatacgag
     3841 ctgttttaaa ctttgtcgtg gtgtttattc tatcggcttc ctttattgcg tatgcacccg
     3901 attatattgc gaaaatcaat gatttttcca aagatgtcag taccgccagt ctgaatattg
     3961 ggacaaaaat tgttcttcct aacacggata gtcaagggaa agacagtgtg gacatgatac
     4021 gtgacagctt attctctgtt caagtaaagc aaccatggct attgcttcaa tatggctcca
     4081 ctgatgtaga agcgttaggg gaagaacgtg tggaaagttt attgtctacc agtccagata
     4141 caaacaatgg tgaagataga gaaaatgtag tgattgaaga aattgaagat catgacaata
     4201 cctatttaac cctaccgaag acgatttcca gattaggaac agtcttcttt ttgtttgtat
     4261 tcaatattgg tatctcgatt tttgttttcc ttctaacagg aatcatgatt ttttcgcaag
     4321 tgctatttat tattttcgct atgtttttgc caatcagttt cctattaagt atgttgccga
     4381 gttttgatgg catgggcagg catgccatta ccaagttatt taatgtgatt atgacaaggg
     4441 ctggtattac cttgattatt accgttgcct ttagtatttc cactatgctt tattcgctgt
     4501 cagcaagttc tccctttttc atgattatgt ttttacaaat tgtgaccttt gcagggattt
     4561 attttaaact gggcgattta atgagcctat tcactttaca aagcaacgac tctcaaaaca
     4621 tgggcagaca agtgtttcgc agacctcgtc aaatgatgaa tcgacaaacc agaagatttc
     4681 aacgcagtat gagtaagatt tttcgtggaa aagcagggtc aacgaagcaa aagacaatga
     4741 caacaaagca gaatcaaacc aataaagcta accatactcg tcaaaatgaa cggactacac
     4801 caaagaaaga aacagcactt tccaatgtga aaaagcaaca aggcggtatc gggcaacgat
     4861 taggtgaaaa gacagcccat gtattggaca cgaaaaatcg tctagtcaat aaaagaaagc
     4921 aggcaaaaca acagatacag gcgacaccaa cccatgtcaa atatgccctt catcaaggaa
     4981 aagagaatgt cactcataat gtaacagact ttaaacaatc tatggcagat accaaagctc
     5041 aacgcaaaca acagcgtatg aaccagcagg agcaaaaacg aaagaacatt gctcaaaagc
     5101 gtttggagct ggataaagca aaagaaaaaa gacgaaaaga aaacccatca tctcctgttc
     5161 agaataacca gcagcatgtt cgcaagcgtc agcaaaccgt cacgcctgtt ttgtcacaaa
     5221 acactaaacg tgaaaatcat cagcgaccat tgaaaaaaga gcaaaaacaa aagacaccac
     5281 cgaagcaatt caatcagagg aagctaaata aaagaacgga taagcgagga caacaaacat
     5341 gaagctaaaa ataacagtga tttgtagtgt ggtcttctct ctcttctttt ttctcttatt
     5401 attcctagtc attttctttg cagatgatac ggacagtggc gaaaacaaca aggattcttc
     5461 tattccacaa ggcggtgtga ccgtttcacc agaagtgttg gcacaccgtc cgttgattga
     5521 aaaatatggt aaggaatatg ggattgaaga ctatgtttct tatatcttag ccattatgca
     5581 agtggaatcg ggcggtacag cagaagatgt tatgcaaagt tccgaatctt tgggtttacc
     5641 gcccaacagt ttgagtaccg aagagtccat caaacaaggg gttaaatatt tcagtgaatt
     5701 attaaccagt gcagaacaac aaggagtgga tatagattct gttatccaat cttataatta
     5761 tggcggtggc tttttaaatt atgtgaggag tcatgggaaa aaatatacct atgaattagc
     5821 cgaacagttt tctaaagaga agtcgggtgg tcaaaaagca gactatccca atcctatagc
     5881 catacctgtg aatggtggtt ggcgatataa ctatggaaat cagttttatg ttcagttggt
     5941 ttcacaatac ctaaccgaca cttccccaac agaatttgat gatgaaaccg tgcaagtcat
     6001 tatggacgaa gccttgaaat acgaagggtt tccctatgtc tttggagggg cgtcgcctac
     6061 cacctctttt gattgtagtg gcttaataca atgggtgtat gacaaagctg gcatttctct
     6121 ccctcgtgtc gcccaagatc agtatgacgc tacgcaagaa atctctatgg aagaagccca
     6181 agcaggcgac cttatcttct ttcattcaac ctataatgca ggcacatatg tgactcatgt
     6241 ggcgatatat ttagagggaa atcgctttta tcatgcaggc gacccaatcg gttatggcga
     6301 cttaagcagt cgttattggc aagaccatct gattggggct agacgtgtta ttcataacta
     6361 agaaaggaat gaatcaaatg attataaaaa tcgagcgaaa agaaaagaag gagaaaccaa
     6421 agaaatctaa aaaaccgagc aagcaaagaa aagttccgat gattaaagtt ggcacgcata
     6481 agaaattaac ctttgtttta tgggttttat tgattggtag tgtcggattt ggaatctata
     6541 aaaattttac tgccattgac actcatactg tccgtgaaac agaaatcatt aaacagcaga
     6601 ttgtcgatac caaccaagta gaaagttttg tgaaatcctt tgcgaaagac tacttttcgt
     6661 ggcaacaatc ccaagaagcc attgataaac gaaatgaaaa attgacccat tatttgacgg
     6721 aagaattgca ggtattaaat gaagaaatga ttcgtaagga tattcctact agttctagtg
     6781 tgaacgatat acaggtgtgg caggtttctc aagtgaatga gaacaccttt gaggtactgt
     6841 tttctgttga acaagttatt accgaagaca aagacaagga aactatatcg tctagctttc
     6901 atgtggttgt ccatatagat gaatcagata atatggttat tatcaaaaat ccgacaatga
     6961 gtaagaaacc tcaaaaatcg gactaccaac ccaaacagct agaaagtgac catacagtag
     7021 atacagaaac gatggacgaa atcactagct ttttagaaac gttcttccag ctctatccta
     7081 ccgccacaga aaaagaattg acttactatg taagtaatca tgtgttacct atgattaaca
     7141 aggaatatgt tttcgaggaa ttggtgaatc ctatctttac cagaaaagat aaccaagtaa
     7201 tcgtgaatgt cgctgtgaaa tatttagatc aagaaacaaa ggcaacccaa atctcgcaat
     7261 ttgagttgat attagaaaaa caagataatt ggaagattgt aaaataacaa atattggtac
     7321 atgattacag atactttgta atcatgtact ctttttgata aaaaattgga gattccttta
     7381 caaatatgct cttacgtgct attatttaag tgactattta aaaggagtta ataaatatgc
     7441 ggcaaggtat tcttaaataa actgtcaatt tgatagcggg aacaaataat tagatgtcct
     7501 tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca tgtgattcta
     7561 aagtatccag agaatatctg tatgctttgt atacctatgg ttatgcataa aaatcccagt
     7621 gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat ggaggaaaat
     7681 cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcaggaaa aactacctta
     7741 acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt ggacaaaggt
     7801 acaacgagga cggataatac gcttttagaa cgtcagagag gaattacaat tcagacagga
     7861 ataacctctt ttcagtggga aaatacgaag gtgaacatca tagacacgcc aggacatatg
     7921 gatttcttag cagaagtata tcgttcatta tcagttttag atggggcaat tctactgatt
     7981 tctgcaaaag atggcgtaca agcacaaact cgtatattat ttcatgcact taggaaaatg
     8041 gggattccca caatcttttt tatcaataag attgaccaaa atggaattga tttatcaacg
     8101 gtttatcagg atattaaaga gaaactttct gccgaaattg taatcaaaca gaaggtagaa
     8161 ctgtatccta atatgtgtgt gacgaacttt accgaatctg aacaatggga tacggtaata
     8221 gagggaaacg atgacctttt agagaaatat atgtccggta aatcattaga agcattggaa
     8281 ctcgaacaag aggaaagcat aagatttcag aattgttctc tgttccctct ttatcatgga
     8341 agtgcaaaaa gtaatatagg gattgataac cttatagaag ttattactaa taaattttat
     8401 tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat tgaatataca
     8461 aaaaaaagac aacgtcttgc atattaagta gttcagtttt ggagtacaaa aagtgaaaat
     8521 gtaaacctaa atgagtatca aattgcaact gaatttgata ctcgtttttt attgttaaat
     8581 caacgtttgt tgttaattta caccacaaaa tgtaagttaa aatgtatata aaaatgcgaa
     8641 cgtgaaataa ttattgtagg aatgcaaatt ggtgtgttaa aatggttaca atttactaag
     8701 caaaaagggg tgcattttcc tatgcaattt cttgagccaa aaaataagaa cgctaagtct
     8761 gtggattggg aaatatcaga gcaagtaagg gtgatagtta agcaatacgc tgaatatgca
     8821 gagcgtactg aaagcgaagc agtggatgaa ttcttattga acattttaga cgataagaag
     8881 tttattgaat ggattgccaa caagcgaagt aataagcgga ttgttgaaaa aatgggtatc
     8941 aaagatagag tgggttgata gacaatgacc aagcttaaaa gattagccac gaaggaagat
     9001 gaaattgtag atgttaaaat tccgatttca aacgaagaat taaaagatag ggctaaacaa
     9061 tatgatttat taactccaaa acgatttgca acaaggtata acaaaatgtt gttcttgcca
     9121 acgtctttta aatggaacgg ttctgaatat cccattcaat ataattactg catcaatccg
     9181 ttttgttgta attttgggaa agaacagcac aaattcaaag atgtaaaagg gaaaccaagt
     9241 cgatataaaa tgactggtag ttccaaagat aaaggtcata aaggtatgta ctgcaatgac
     9301 aatcctattg gcagaggtgt atcgcaaaac tgtacagtaa cccctttatc taattggtct
     9361 gttgttgaag aaattaaacg ccttatagaa atcaatagca tccaagatgt tgagccagat
     9421 tatcaatttc ataaagaagg ctgctcggaa gaagaatcaa ccccattcaa tgagccgaag
     9481 caattttaca aaagggggaa aagtaggggc aaatctcaac gctatcaatg taaggcgtgt
     9541 aagaagttca ctaatgttct accaaaaaga gaagaaacca ctacttatca ccaacaaaag
     9601 aacaccattc ttccaatgtt cgctaagatg gttgttggaa gggtttcagt cagtcgtact
     9661 tgtgatattt taggaattgg agttggaacc tactatcata agctggagtg gctgtatagg
     9721 cgttgtttgg agtttttaga acgatatgag acacaacccc ttcaaacaaa gaaatttaac
     9781 gagatgtggc ttaatacgga caagatgcac tactacctaa ataacgtccg taagaaagga
     9841 caaggttcaa agaaatatac ggggtttgaa gaccttaata tgcaaacgta tatagttgta
     9901 tctgctgagg tcttatcaag atatgtgttc cgttctgatg ttgcctatga ttggaatatt
     9961 tcaatggatg aattaaatga agatacaaga aaattcaaag aagaccatct caatactttt
    10021 agtcgtaaaa atgataggtt agattggtct tattatccac aagaaccatc cgctaacgat
    10081 agcgaaaata ggaatgctta tcttcacgaa ttagggaaaa ttactaatag gagtagattt
    10141 gtcgatggtt taaatgttga tgctccttat actacaactg ctcactattg gttaattaaa
    10201 caaatggtta atgccgacga atggcgaatg attagtgatg atgacttctc tattagaaac
    10261 gccttttata gagtattcac gaaagaatta aggttgagcg atgcacatca ttttatttgc
    10321 caagttaaca agacgaaaag cagaaaacaa tgcttaaaag aattcggaca agcaaaagca
    10381 gaactgctcg attggggaga tatcagagga ttcaaaacaa agtttctacg gactttggca
    10441 tctcattatc taactgaatt actaacaagt catcaattcc acgaagaggc tattagtaaa
    10501 gatggagaaa gatatcgcaa atatgccgat aaccctatca aacatccatt agccactaag
    10561 gataaaggat tttattcagt tgattgtaga acggatttat ctgctctgga accaaacgaa
    10621 atcgcaaaga tgctattaaa tgttaatgac cattcgacca atagctttat tcaacagata
    10681 agacgttata tatcatctct tgaaagacca ttaacaacag ctcgtggtga taagaagagc
    10741 tatatttacg ctaacttcaa tccgaagtat gctcaatttg cgattacaat tctaagaact
    10801 tactacaact tttgtcgccc ttttaaatca gcggataaaa aggttttgac acctgctcaa
    10861 agattaggga ttacagataa acaatttgat tggaaagata taatttattt caaatgacaa
    10921 aaagagcttg gagtaatccc aagctcttgt gctttatctt tactgttctt cttcaattaa
    10981 cgcacccgtt aactcaataa gttttttctt tcatatacat gaaaatagta gttatacgga
    11041 tttttatcat tctttatccc tttttgggca aatacctcat tccattcctc ataattcact
    11101 tctggaaaaa aagtatctcc ttcgaattca tgatgtattt ttgtgatgta cattttctca
    11161 acataaggga aaaacaaatt ataaatctgt tctcctccga aaataaaaat ttcttcttcg
    11221 tttttacata actcaaaaac atcttctatt gaatgaacaa tttcacaacc attaaaggta
    11281 aaccccttat ctctcgtcag aataatattt cttctgtcag gtaaggctct tccgattgat
    11341 tcaaggttct tcctacctaa tattatcgga tgtccctttg tagtattttt aacatattcc
    11401 cagtccttgg gaatcctcca aggaatgtca ttctctttgc caatcactct attcttatcc
    11461 atcgcagcaa tcaaagaaac tttcatttat aacacagcct ttttaactaa tttaccatct
    11521 aatttcttat ttctatcaaa aaaccttttc tcattatcct gcccgttagt tgaataagca
    11581 attttttcta taagttatat tatcataata ccacaaaatc acaaattttc attcaattat
    11641 cattttcaat tgcattttat aaatcatttt gtatgtaatt tggtatgtaa gttaaaatga
    11701 aaaaagtagt ccattttgaa gtacattttg tcattttgta ctccaaaact gaactactta
    11761 cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca
    11821 gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag
    11881 attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat
    11941 agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct
    12001 ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc
    12061 cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat
    12121 gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa
    12181 gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg
    12241 ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc
    12301 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt
    12361 tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggt
    12421 tgcgaacaag gattatatgg ttggaatgtg acggattgta aaatctgttt taagtacggt
    12481 ttatactata gccctgttag tactccagca gattttcgga tgcttactcc tattgtactg
    12541 gagcaagcct ttagaaaagc tggaacagaa ttgttagagc catatcttag ttttaaagtt
    12601 tatgcaccac aggaatatct ttcacgggca tataacgatg ctcccaaata ttgtgcaaat
    12661 atcgtaaata ctcaactgaa aaataatgag gtcattatta ttggagaaat tcctgctcga
    12721 tgtattcaag attatcgcaa tgatttaact ttttttacaa atgggcttag tgtttgttta
    12781 gcagagctaa aaggatatca ggttaccact ggcgaacctg tttgccagac ccgtcgtcta
    12841 aatagtcgga tagataaagt aagatatatg ttcaataaaa taacttagtg cgttttatgt
    12901 tgttatataa atatggtttc ttattaaata agatgaaata ttctttaata tagatttgaa
    12961 ttaaagtgga aaggaggaga ttgttattat aaactacaag tggatattgt gtcctatttg
    13021 tggaaataaa acaagactac gaatacgagt ggatactata cttaaaaatt tccctttata
    13081 ctgccccaaa tgtaagaacg aaactttaat taatgttcaa aaaatgaata taataacaat
    13141 caaagagcca gacgccaaga cgcagagccg ataatttgag aaatgaaact ctcatcttat
    13201 cggctctttt tgtttatctg aattttactg actagccttc aatatttctt gcttcattga
    13261 taccttttgc tagtgcttcc attaaggata gttctttgtc tgtaaagcta tccatgtatt
    13321 tttctacctg taaccttcga gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat
    13381 caatagatac atggagtaat gatacaagat catacagcac ttgtgtactt gggtgttgtc
    13441 ctttgttttc tatatttgtt aaataacgtg ggtcaatttc aatcattgtt cccacttgtt
    13501 cacgagtgag accccgtttc attcgagcct ctttaatcgc taaaccaacg gctctaaaat
    13561 cgtatttatc ttctttttta cgcatactaa atcacctcta tacattttac tgttcctata
    13621 taaccataaa caggtataga aaaacatgtt atatagttta tgagttccta tagaacactg
    13681 tggaatttat taatgcgatg aatccaattt caaatagaaa ggtcgctgat tttatttacc
    13741 tatacaactt tataaaattt agtcatactc ctagaaagta gataaaattg gcgacttaat
    13801 ggagggaaaa gtcgccatga gaaagataat ctgtcatcta gttctcttaa ttaagttctt
    13861 atcaaaaaaa cttatccacc tgtactggat agatatgacc atcaatgtgg ttttgcagat
    13921 tacatagata gaaaaatcaa agcatatgaa cagagaaaac aatctgttta tgtgcttttt
    13981 tggtcactta ataaatatct tttaggattt ctcttcattt atgcaacaaa tcccccgttc
    14041 gtgtcggggt ttagtatgaa gggagataaa gaagcacatg ccactccctt tcacgacaga
    14101 aagggggtga aaagcatgaa accatcttct tttcaagaaa ccatagaaaa tcagtttgac
    14161 tatatctgca aacaagttat tgaagatgaa cgaaaagatt acttcaaaca tctagacaga
    14221 ctatcagcaa aagaagtctc gttttctgat atagataact accttgtcaa ctctttttct
    14281 actgttgata cgtacgtcac tgatttccaa ctatttaaat tatttggatt aagaatcaat
    14341 gttgaaagtg atttattaag tgaagccctg cgtcatcttt cagagaaaaa acggaacatt
    14401 attttacttc attactttat ggatatgagt gatgtggaga ttgcggagtt actaaatttg
    14461 aatcgttcga ctgtttatcg tcatcgaata agtggactag ccatgattaa agaattcatg
    14521 aaggagtgta aagaatgaaa acagcttatc caagggttcc cttccccctc attgtaaagg
    14581 cgacagatgg cgacgtggaa gcaattaatc agattgttaa acattatcga gggtatacct
    14641 caaaacgttc attacgtcgc atgacagacg aatatggaaa ttctcatatg gttattgatg
    14701 aaaccctacg tggcagaatg gaaacgaaac tcatctctaa gattttatcg ttcgaaatta
    14761 aatagtccca ttttttcgtg gaagcgtgtc gaatcgtatc cacgcttccc gatttgggag
    14821 gtttgttatt ccatcaaagc atattgtgtg ttcagtaggt tttgataggc taacaagcca
    14881 aacgttcttt gacaaccaaa taaaggcaat cgaatacgtt tcggtaagaa aagagctgat
    14941 gaactaacac gccatgacct acaaaattag tgagcgattc atgttagtaa ttcgaaagta
    15001 atctttagca ggattgctgc catgactttc ttatcgtgat aatgatactc ccgtatagcc
    15061 atagtccgag cgttagaagc gtcacaggca atgggtacgg ctacatgaga accatgcaga
    15121 agtgaaattc ctgtgagctt tgctagagct gttcgattgc taagatattc ttctcttgtc
    15181 agtgtattat tataaaacta tttgatataa ttataggtga atattggagt tttcctagcc
    15241 tataattggg aaaggagtaa taatgataac ggtcacaaaa aaggatttag ttgagctagg
    15301 atatggaact tctttttcag cagacatcat aaaaaaagca aaagaattga tgattacaaa
    15361 agggcatatc tactatcaat ctcgaaagct agatagagtc cctcgtgaag ctgttgaaga
    15421 aattctagga attcggtttt caaatgaaaa caggtctgag tagtttttgc acagtaagga
    15481 gtaaaaacta tggttaaaga cccaataaaa aaagcaaaga atggaaccta ttattttcgt
    15541 gcaaatctag gttatgattc taaagggaaa aaaattcaaa aatatcgtag tggattcact
    15601 actaaaaaag aagcaagaga agaatattcg aaactgctat tgatgaaacc agaagaatta
    15661 agtgagaata aggataaaat gacgtttaaa cattatatct ttgaaatttt ccttccatgg
    15721 tataagacac aggtaaagct cagaacatat gaaaatcgct taccaactat taagaagcat
    15781 ttctcttatt ttgataaaat ggctgtttcc gatattgagc ctattgatgt gcaaaattgg
    15841 caactaaaat tatcaaagaa gtgtaagtct tcttatgtaa gagctgttca aggactattt
    15901 tcagttgcta tggatcgagc aattgtcctt gggataacca ccacaaatcc atctaagatt
    15961 attggcaatg tgaaaaagca aaagtctaaa attgaatttt ggacaaaaga agaatttgaa
    16021 aaagtaatct ctctaattta caaagaagat tactatcaac actttttatt tatctctctg
    16081 tggtttttat ttatgactgg aatgcgaatt ggtgaagcta ctgccattca atgggaagat
    16141 attgattttg actcaggagt actaaccatc aacaaaacac tctattacaa gaatcaatcg
    16201 aattatagat ttgttgaacc aaaaacaaag gcgagcgttc gtcatattgt attagataaa
    16261 tgcacattaa cttatttatc cgaatggaag gaagtacaac aaaatctaat tcagacagac
    16321 tttgtaatga gctataacgg tattcctacc caaaaacata cactatcata tgctattgaa
    16381 cgctatgcta agctggctgg tatccatcga atcagaatcc atgggttacg gcattctcat
    16441 gcttccttgt taatcagtat gggagaaaat ccacttatca ttaaagatcg tctaggacac
    16501 gaagatattg agacgacttt aggaacgtat ggacatcttt atcctaacag taatttcgag
    16561 gtagctaaca aattaaatgg agccatttcc tttaaagaag caaaacaaaa ttatgatact
    16621 tcaccaaaga atcaatttac tgtagactac ttgcgaaaca aagaaatgaa aaaagtgcaa
    16681 taaaagtgca gtaaaaagat aagaagagcc agaaaccctt ggtataagag gatttctagc
    16741 tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg tcatacacga
    16801 tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc aagacttccc
    16861 atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca cggataccga
    16921 ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc cctggaagaa
    16981 cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga
    17041 tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca agaacacgaa
    17101 taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc atgcctaact
    17161 gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg atcaattcaa
    17221 attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc tgtgctgttt
    17281 ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa gagacgcctt
    17341 cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca ttcccaatga
    17401 ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag cgatctcttg
    17461 catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt acttgttccg
    17521 cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg
    17581 ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga ccaagcaaga
    17641 cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata aagttatcca
    17701 ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc aattcgattc
    17761 cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta cgtgttgtgt
    17821 cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag ccttcaggaa
    17881 ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt gtttctttaa
    17941 acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg cggtttttag
    18001 caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag caaatcccta
    18061 atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc tcatcatata
    18121 cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt acttcttctg
    18181 cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata cgtcttgtga
    18241 tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg ttcgtcatat
    18301 cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca aaatagtcaa
    18361 actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg attttcagtt
    18421 ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg taaatttttc
    18481 aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg atctccgata
    18541 gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa cgcaccaaaa
    18601 cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac agaaaaatca
    18661 aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt gatcccctca
    18721 acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat atcatacaca
    18781 tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg gttcaagaaa
    18841 tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat gattccacgc
    18901 tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt
    18961 cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact ttttccaacg
    19021 gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt atgaagaaat
    19081 aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat cagatgagca
    19141 agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa acttttgata
    19201 ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc tcgccattct
    19261 tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg cgttctccca
    19321 tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga gagattgccg
    19381 atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga cttgcgcacg
    19441 gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa gacatgacaa
    19501 atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg gcttttgagt
    19561 taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt gaaacagtgg
    19621 attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct gatgaaggaa
    19681 agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca tttgcgacac
    19741 agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg caaggaaacg
    19801 caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat gaagcagttc
    19861 atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga aaaaaagttg
    19921 tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca ctgacaattg
    19981 tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa acatttggta
    20041 atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg aaagaataac
    20101 tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta cgggtacgta
    20161 tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga
    20221 aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca gaacttgtat
    20281 agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac tttccttttt
    20341 tctctattct tttcttgggg gttttggtac actgataggg aatttagata gaagggagcc
    20401 aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc agtaacaact
    20461 gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag aaaaaaagtg
    20521 ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa tgtgatcaat
    20581 cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt cttctccaaa
    20641 ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat gttatgcaat
    20701 tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca ccgctcagtg
    20761 gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt agacagcggt
    20821 ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg ggataacgga
    20881 cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat
    20941 ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt agaagaacgt
    21001 ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg gaaatttgtt
    21061 gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt actcaaacat
    21121 aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg
    21181 atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt cgatggatta
    21241 aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag ttttgatatt
    21301 ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag aaaagttccg
    21361 aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt gacaaagggc
    21421 taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg ctttttttat
    21481 ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt ataataccga
    21541 atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa aagagggatg
    21601 gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg aatggaaaga
    21661 ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac atgacaaatg
    21721 aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc gatttgagtt
    21781 ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac acgaccacat
    21841 tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg
    21901 gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga tttcagatcg
    21961 agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg gtaattggtc
    22021 aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga
    22081 ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc gcggcaggag
    22141 cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag aacatcgaag
    22201 atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac cgagaaacag
    22261 tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat agtctagaag
    22321 ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta gaaatgtgct
    22381 acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc gatgaagcaa
    22441 gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga
    22501 tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt ttgactgcac
    22561 gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg
    22621 aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg
    22681 atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca aaaggtgagt
    22741 cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa ttattatata
    22801 aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac atcatcacag
    22861 aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa atggaactaa
    22921 accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct gtgatgcaaa
    22981 ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca gcatgggttg
    23041 cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta atcgggtttc
    23101 ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc atcaataacg
    23161 gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac
    23221 aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag
    23281 ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc gaaatagcaa
    23341 aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc ggagcaaagg
    23401 tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc aaagcttccg
    23461 gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct acaagaatcg
    23521 gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat taattttttc
    23581 aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta ttgaagcgtt
    23641 agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg taacggaatc
    23701 aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa cgaattgtgc
    23761 cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta cgcatctagt
    23821 cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac aagtaatggc
    23881 agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg tgacgaaggc
    23941 aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat aactcatgtt
    24001 cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt tacagaaatg
    24061 aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt atttttaggt
    24121 gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc agacacaaca
    24181 actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa ccagtcagct
    24241 tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa tgatggcttc
    24301 caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca agcattgaat
    24361 gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat ccaagaacaa
    24421 gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga tggattagat
    24481 aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg tgttgcagca
    24541 gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc
    24601 gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga attaggaaaa
    24661 gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa aggacgttca
    24721 attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc aggcggtaca
    24781 ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg
    24841 gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa cggcgaaaag
    24901 aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga agatttggca
    24961 caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg attgaaagaa
    25021 gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc agttgatgaa
    25081 gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga agaaaactaa
    25141 aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg attagaacaa
    25201 gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca actccctgaa
    25261 gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca atcgttgccc
    25321 tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat
    25381 gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca atttacaagt
    25441 aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat ctacattgat
    25501 gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc gtgggaaaga
    25561 agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg tccatcagca
    25621 ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa gcgagcctag
    25681 tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag tttctgaaca
    25741 gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg gaatggaaca
    25801 acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt ttgatgagcc
    25861 gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga aggaattagt
    25921 caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca aagcagtagc
    25981 tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt
    26041 aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca aaacgatgaa
    26101 aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa
    26161 tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg
    26221 tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga ctggtttgcg
    26281 aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata aaaaaccccg
    26341 aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat acgggttagt
    26401 cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa aaccttacag
    26461 taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat tgatcgaaga
    26521 atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag gcggaaatca
    26581 gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa
    26641 tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc tgatcgaaca
    26701 aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt
    26761 ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg atccgaaaga
    26821 aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag aagcacgaaa
    26881 agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc cgtaatttat
    26941 tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg atggctgctt
    27001 ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt ggttcgcaaa
    27061 gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca ttaggttttt
    27121 ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca ctttgcgggt
    27181 ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt gttttgcttc
    27241 cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt ccaggtttac
    27301 ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac tatatcttat
    27361 tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc tcaagtttgg
    27421 actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta acacaagctt
    27481 ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt attttcgttt
    27541 ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga ttgaatcctt
    27601 ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg atggtcattt
    27661 ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct
    27721 ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc ttgcttggtt
    27781 ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg aagatcggtg
    27841 gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt
    27901 tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct aaagtcttcc
    27961 aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg aatcaaaacc
    28021 atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc ttacttctac
    28081 actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt ctgaacgaag
    28141 cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta gttccatcgt
    28201 tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg ctattttagt
    28261 gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag tcagtttgcg
    28321 tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt taggtgtttt
    28381 cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga atctggggta
    28441 tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct ttaaaaatac
    28501 attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga tcatcttcaa
    28561 gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg ccgacacatt
    28621 aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct tgttaggcgg
    28681 tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt
    28741 tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc ctttaggggc
    28801 aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg gcgcacaatt
    28861 accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt tgctgacaat
    28921 cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg ggaagaacta
    28981 tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg
    29041 tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat aatcatgttt
    29101 aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc agatgccgat
    29161 aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc aggattgaac
    29221 gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg tgtggaatca
    29281 gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg
    29341 atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg tgtgtttcct
    29401 aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg taaagtcgta
    29461 tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca ccaaatgaaa
    29521 acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa
    29581 gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga tataacaaaa
    29641 gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga accaggagac
    29701 ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca cacagtcctt
    29761 gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa cgatattttc
    29821 aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg cgttgaccag
    29881 ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt agtagatttt
    29941 gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat tgaagaattt
    30001 gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct aatgattaca
    30061 gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga atatgtaccg
    30121 ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg atattattca
    30181 gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc
    30241 ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc aagttaagcg
    30301 agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa attgattttg
    30361 gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat gctgtaatga
    30421 tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat gcaggaaagc
    30481 tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga ttccactact
    30541 atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc ggactagggg
    30601 cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag
    30661 atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg atcggtccga
    30721 atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg cctgactatc
    30781 aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa ggcgtatata
    30841 tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct cggacaatag
    30901 gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct cacagcggat
    30961 taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa gcgaatctga
    31021 gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa gcattggtta
    31081 aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca tattgaagcg
    31141 aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg agctaaatat
    31201 attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg tatgttaggc
    31261 tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat gggaatgcca
    31321 tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa attgattcga
    31381 gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt actagcacaa
    31441 ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca ttttcctcca
    31501 atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa gggctatacg
    31561 gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt gacagagacg
    31621 ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc tctttattac
    31681 ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga ccatttgatc
    31741 accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat gatcgaagta
    31801 ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct gcattaagta
    31861 ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga cttattattg
    31921 caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt ttaactctga
    31981 gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtg
//
LOCUS       DAZKAS010000016        31938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000016 DAZKAS010000000
VERSION     DAZKAS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.016"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..456
                     /locus_tag="ACG8H7_000631"
     CDS             214..456
                     /locus_tag="ACG8H7_000631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982755.1"
                     /translation="MSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFK
                     RYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(826..1440)
                     /locus_tag="ACG8H7_000632"
                     /pseudo
     CDS             complement(826..1440)
                     /locus_tag="ACG8H7_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1680..1925
                     /locus_tag="ACG8H7_000633"
     CDS             1680..1925
                     /locus_tag="ACG8H7_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982756.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2023..2244
                     /locus_tag="ACG8H7_000634"
     CDS             2023..2244
                     /locus_tag="ACG8H7_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982757.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            2566..3246
                     /locus_tag="ACG8H7_000635"
                     /pseudo
     CDS             2566..3246
                     /locus_tag="ACG8H7_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            3386..3925
                     /locus_tag="ACG8H7_000636"
     CDS             3386..3925
                     /locus_tag="ACG8H7_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982758.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            4945..6114
                     /locus_tag="ACG8H7_000637"
     CDS             4945..6114
                     /locus_tag="ACG8H7_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP8982759.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            6587..7141
                     /locus_tag="ACG8H7_000638"
     CDS             6587..7141
                     /locus_tag="ACG8H7_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8982760.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(7314..7751)
                     /locus_tag="ACG8H7_000639"
     CDS             complement(7314..7751)
                     /locus_tag="ACG8H7_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8982761.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            7799..8746
                     /locus_tag="ACG8H7_000640"
                     /pseudo
     CDS             7799..8746
                     /locus_tag="ACG8H7_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            9017..9452
                     /locus_tag="ACG8H7_000641"
                     /pseudo
     CDS             9017..9452
                     /locus_tag="ACG8H7_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            9468..10301
                     /locus_tag="ACG8H7_000642"
     CDS             9468..10301
                     /locus_tag="ACG8H7_000642"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8982762.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            10546..11571
                     /locus_tag="ACG8H7_000643"
     CDS             10546..11571
                     /locus_tag="ACG8H7_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8982763.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            11709..11834
                     /locus_tag="ACG8H7_000644"
     CDS             11709..11834
                     /locus_tag="ACG8H7_000644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982764.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            11859..12974
                     /locus_tag="ACG8H7_000645"
                     /pseudo
     CDS             11859..12974
                     /locus_tag="ACG8H7_000645"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            13057..13953
                     /locus_tag="ACG8H7_000646"
                     /pseudo
     CDS             13057..13953
                     /locus_tag="ACG8H7_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            13969..14793
                     /locus_tag="ACG8H7_000647"
     CDS             13969..14793
                     /locus_tag="ACG8H7_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982765.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            14825..16135
                     /locus_tag="ACG8H7_000648"
     CDS             14825..16135
                     /locus_tag="ACG8H7_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFP8982766.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            16283..17542
                     /locus_tag="ACG8H7_000649"
     CDS             16283..17542
                     /locus_tag="ACG8H7_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8982767.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            17552..18424
                     /locus_tag="ACG8H7_000650"
     CDS             17552..18424
                     /locus_tag="ACG8H7_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982768.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            18436..19266
                     /locus_tag="ACG8H7_000651"
     CDS             18436..19266
                     /locus_tag="ACG8H7_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982769.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            19306..21489
                     /locus_tag="ACG8H7_000652"
     CDS             19306..21489
                     /locus_tag="ACG8H7_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP8982770.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            21550..22770
                     /locus_tag="ACG8H7_000653"
     CDS             21550..22770
                     /locus_tag="ACG8H7_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFP8982771.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            22767..24227
                     /gene="gtfA"
                     /locus_tag="ACG8H7_000654"
     CDS             22767..24227
                     /gene="gtfA"
                     /locus_tag="ACG8H7_000654"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFP8982772.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            24304..25410
                     /locus_tag="ACG8H7_000655"
                     /pseudo
     CDS             24304..25410
                     /locus_tag="ACG8H7_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            25732..25941
                     /locus_tag="ACG8H7_000656"
     CDS             25732..25941
                     /locus_tag="ACG8H7_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP8982773.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            25934..26083
                     /locus_tag="ACG8H7_000657"
     CDS             25934..26083
                     /locus_tag="ACG8H7_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP8982774.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            26237..26902
                     /locus_tag="ACG8H7_000658"
     CDS             26237..26902
                     /locus_tag="ACG8H7_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8982775.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(27186..28271)
                     /gene="xerS"
                     /locus_tag="ACG8H7_000659"
     CDS             complement(27186..28271)
                     /gene="xerS"
                     /locus_tag="ACG8H7_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFP8982776.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            28605..28838
                     /locus_tag="ACG8H7_000660"
     CDS             28605..28838
                     /locus_tag="ACG8H7_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFP8982777.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            28835..29242
                     /locus_tag="ACG8H7_000661"
     CDS             28835..29242
                     /locus_tag="ACG8H7_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP8982778.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(30379..30897)
                     /locus_tag="ACG8H7_000662"
     CDS             complement(30379..30897)
                     /locus_tag="ACG8H7_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFP8982779.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            31264..31512
                     /locus_tag="ACG8H7_000663"
     CDS             31264..31512
                     /locus_tag="ACG8H7_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982780.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT        10984 a         4803 c         5842 g        10309 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatcc aatagaattt gacaatgatg aaagattgct gctaatatat
      121 gcgtttatgg atgaaaattc tatcttttca actgataatt caacttatag aaaagtagtt
      181 caatgggaaa gtgataacaa tttaaggagt tacttgagtg atagagtatt tgatgtacta
      241 gaaaaattaa gtaaatcggg aattttaaag gaagaatacg attcttcttt tgatgagtat
      301 ccatcacgct atattttgat ttcagaggac tataagaaat ttaaaaggta ttttttaaaa
      361 aatgaaaaaa agatatctgt tattatggag aaatacaaag ctccaataca ggctgatcca
      421 ttttccaatt tggatgaatc agatttaccg ttttaaattt taaatagata atttcatttt
      481 ttagtagcag aggtataaac ttttttaatg tttgaattga aaagaaatgc taataggtcg
      541 actttatgtt tctcttgtgc aatcgtaaat gcgggggacc ataaaattga tctattatcg
      601 agtgtgtata aaataaaatt caaatcttat tagtttacat aagatacatt atactaagtt
      661 gtcaatgtta aatgccttta aataaggacg atttatgcat caattatatt tatgttaact
      721 aaattctttt taacataaat gtaatcaact tagctagatg aatctaaaat gattgtctgt
      781 attgatgaca aagctaatta ataattacgt gattcaaaaa agaaatcata caattagttg
      841 gtcgatttct ttttttattc ggtaaattgt ttgcctagtc acattgtagt ctgttgcaat
      901 ctttgagata gcaaccccat tctcaagatc ctgaacgata ctcctataga caaggcgacg
      961 ttgaggatct ttggtgtctg cactgtataa cttaggacga cctttataga cgccgtttgc
     1021 tttagcgatt ttaattcctt gtgcctggcg tcgtttcgat tcagtacgtt cttgttcagc
     1081 aatcatggct gagatttaaa tgattaagtc tttgatgaat ttatctagga gaggatttcc
     1141 aattgcttca gccatgatcg gtaaactggt gataataagt cgcacattct tcttctttag
     1201 ataattcact gaatcgataa tctcgtcata attgcgtcca agtcgatcaa tggcttctac
     1261 aataaaaata tcttgctctc tgacaaaatc taaagcttcc tggaaaatcg gccgatgact
     1321 aatcgtcgct ccagattgtt tttcgataaa aattttttca gcgccaaact ttttcatttc
     1381 ttcaatttga cgttgttcat tttgatcgat ggatgaaact ctaatataag caattttcaa
     1441 atttattccc tacttttcta taacgtttat aatacaccct aatcatacgt tagaactcta
     1501 aatttttcaa tgttatattt ttgtctattt agggtaaact ctaaatagac aaacggtttt
     1561 tgaattctaa aagttaaata gtttataata gaagaaaaag aatggggtga cgatttctta
     1621 gattgaaaag gaaagataaa tattatagat tattactttt gacagctgaa gcaggatgga
     1681 tggattttcc agaagttgta aaagaagttc aagagatagg tgcaactatt tctgatagcc
     1741 gctcctctcc tatgaactat caggatgccc atggaattaa aataattgat caagagagaa
     1801 atgtcagaaa atacacaaca ataaatgagt gtgtaattaa tgaaaatctt tgtagggcac
     1861 cgataacgaa acacattaag aatagaagca aagcaaaaga cggaagaact tttactacct
     1921 tctaagtttt tttcatattt gtttgctata cttagatcta aggaattaga ctttcgctgc
     1981 ccattattta agtttgaggt agcgtcttgc gagggatcaa taatggaaga agaaaaaata
     2041 tttgaaaaac gctgggaatt ggcttcagtt gaacaaagag caaggtacca taatctaatg
     2101 tcctcttacc gtaatattga ttggacttac aaagaaaaga agtacttgct ttggttatgt
     2161 cagttagatg taaatacctt tgaaacattt gaagtgattc tagataagat taaaaacagc
     2221 aatgaaaaaa gagctgatct ataatggttc gctcttttta ttttattaac tctttggtta
     2281 ctatatgtaa aatacagctt catattacaa taaatttcag gatacaaatt tatttaagca
     2341 tatataaata taattgaaat aggacataaa tatatgagta tgagaaaggt gattccatgt
     2401 ttaataaatg ggcaaaccac ccaatgaact cgagaggtag agataatttg aaggagacgg
     2461 acaagttctg ggaagaagta tatagtaaaa acggttctgt tgcaaagttt tccaaaaaat
     2521 ctattttagt gtaaaattga gaaaaaagac agagaggaca gagtaatgaa tcattttaaa
     2581 ggcaaacaat tcaaaaaaga cgtcattatt gtcgctgttg gttactacct gcgttacaat
     2641 ctaagctatc gtgaagttca ggaattgtta tatgatcgtg gaataaatgt ttgtcatact
     2701 acgatttatc gttgggtata agagtacagc aaagtcctct attatctttg gaagaagaaa
     2761 aatagacaat ccttctattc atggaaaatg gacgaaacct atatcaaaat taagggacgt
     2821 tggcattatc tttatcgtgc aattgatgcg gacggcttaa ccttagatat ctggttacga
     2881 aagaaacggg atacgcaagc agcctatgct ttcttaaaac gactccataa acagtttggt
     2941 gagccgaaag caattgtgac cgataaagca ccttctcttg gctccgcctt tagaaagtta
     3001 cagagtgtgg gtttatatac taagacagag caccgaactg tgaagtatct taacaattta
     3061 atagaacaag accatcgacc tattaaacga cgaaataaat tttatcaaag tctccgtaca
     3121 gcctcttcca cgattaaggg catggagacc attcgaggaa tatataaaaa gaaccgaaga
     3181 aatggaacgc tcttcggctt ttcggtgtct actgaaatca aggtattaat gggaataaca
     3241 gcctaagata tttggagttc agagagggcg cgtttgattt tcaaacttcg caacagaacc
     3301 gagaaccttg ggttttcaag tgctttttgg ttcattaatc ttaaccatag tgtttttaaa
     3361 gtggaaaaca agcaacaaaa aaagtatgtg gagccttgta ggaatagttc ttattatttt
     3421 tactgtgact gcagcaacag aagcaaatta ttcgcataaa attcagtcgt ataaaggtcg
     3481 tttagttatg accaaaaaag atgttacacg ttatacatcc tcaagaactg gaggcctata
     3541 tgactatgca ccattagatg cattgaaata taaagatcat ttaaagaaac atgaagtaaa
     3601 agttggtgat cggtgggaga atgcatctca taaagcatat aattccaaaa tagtctatac
     3661 agtagaagat gccgatgagc aaaatatcac attacctgta ttcaattatt ggggaacagt
     3721 tgtgaaagta aatgggaaaa agagcactat agaaaatgac aatggtttag tgtctattaa
     3781 aggtacgggg aaaagaacta tagttgaggt ttcatctacg tatccaacta cgatgattct
     3841 atctttatta attagttcaa taacttttat attcttattt acacgttatt taataagatg
     3901 ctgcggtgaa atgaaaatag attagataga tattataaat atgttgattc tattttcata
     3961 gcaatggaga aaatacatta gcaatatata aaagaaggta tcaagagtca cagtgaattt
     4021 attccgaaaa gtgaaaagag caaatataaa atttaatgaa aatatagaac taaataggaa
     4081 aacgtagacg taattttcta cgttttcctt ttcatatata ttaaatatta cggaaaatta
     4141 tataaactaa tttagtgttt tgtttgtaat tcctaatttt gctccacttt tagataattt
     4201 ttaggtctat ttaactcatc agacattcta tgctcatttt tctccaaatt tgtttcaact
     4261 ctttcgtact taattttgcg tatttgaaaa ttcttctttt agagtacttt tattacgata
     4321 ctagtatgat atatatctat agcaaacagt taaaatcttc ctaaaataag cgaatagcat
     4381 tcctaaaacc tataaaattt ttagatgaag cgacaagtgg acaattaata aactacttga
     4441 acatgaaatt atggatcacc tttcaaattt agaaaatgga attcttatac ttattgcgca
     4501 acatttattg attgcaaaat tatgtgacaa aaaaaactag tagaagaaag aataatggct
     4561 tgataataaa gtactaccta ttaaaggtag tactttattt aatttagtat aaattaacat
     4621 actactatat tttaggtttc atattatata aattaataat atactaccat ttgttggtag
     4681 tataatttaa aggtaataag ttattctttt attactatgt ttgggtttta tagcaaagaa
     4741 agtttaataa gagtttggac ctagcaaaca atattaatga ggtcattaaa tcttattatc
     4801 tttagtgata taacgaaata tctacacaga caaaatgata gcgtaaaata ttttgtgtaa
     4861 atagatattt agggtagaaa aaaagaaagt ccattctgta aaattaaagt taccacaacc
     4921 gaaattttat aggaggactt tctcatgccc aattttacta cagaaattat ggaaacacta
     4981 atcaataaag gtgatttaga tgaattattt cgatgtcatt tagaattagc tgttaattca
     5041 ttattgcagg ctgaattaac agcttttctt gattatgaaa aatatgatcg tgctgggttc
     5101 aattcaggta attcccgtaa tgggaattat tcacgttcat tcagaacaga gtatggagaa
     5161 ttaaatttgg taattcctag agatagaaat ggtaagtttt ctcaacaaac tttacctgct
     5221 tataaaagaa ccaatgactc tttagaaacc actattattc agctatttca aaaaggaatt
     5281 actatgtctg aaatctctga attgattgaa aaaatgtacg ggcatcacta tacgccacaa
     5341 accatttcaa atatgactaa aatagtttct gaagatatta ttgcctttaa agaaaggtcc
     5401 ttagaatcca gatactctgt catttttatg gatgctactc acattccttt aaaaagacaa
     5461 actgtttcaa aagaagcagt ttatattgta attggcattc gattagatgg aaccaaagag
     5521 gttcttgggt ttagtcttgc tccaactgaa tctgcttatg tttggaaaga gattcttcaa
     5581 gacctaaaag accgtggttt aaaagaggtt ttattagtcg taactgatgg tttaagtggc
     5641 attaacgata gtattcatag tgtttatcca aatgctcaat ttcaacaatg ttgtgtccat
     5701 atatctagaa atatcgctca taaggttcgt gttagtgatc gacaagaaat ctgtagcgat
     5761 ttcaaattag tttatcaagc aagttcaaaa gaagaagcaa ataatcaaat aaggtttatg
     5821 atagataaat ggaaaaagca gtatccacga gtggttaaat tgctcatgaa tcctgctata
     5881 ttaacctttt ataatttccc tccgtcaatc agaagaacta tttattcaac taatttgatt
     5941 gaaggtttta acaaacaatt aaagaaatat accaaaagaa aagagcaatt tcctaatgaa
     6001 gaatctctgg agagatttct agtatctcaa ttcaacaact ataatcaaaa gtttctatgc
     6061 cgagttcata aaggttttaa agaaatacat gatacattag aatcaatgtt ttaacttgat
     6121 caacagaatg ggcttttcca tttacacaaa attattgacg ctcccgacaa aattatgatc
     6181 ttgcgtaaag ttataaaata ttaataaaaa tattttatta atgaaaagct tggtagcacg
     6241 gttattcata tgctaattta tttcacttta tagtaagggg agttcttagt atattgttca
     6301 aacttaaatt tcatataatt taattatgta aattagctat tctaagaact tctacggata
     6361 actgcccgtc taaaatttaa ataagagctt agaatcttca tataagtaag gttaattttt
     6421 ttgtacaaaa aagtcggaat attctgagtt actgaaatga tataaaatca taaactaaat
     6481 cccttgattt tattgacttt caaaagttta tatatataag ttaatgatta tggtaaaata
     6541 tatacataat cattaactca gaatattttt aagaagggaa acaatagtga gctataatgt
     6601 acaaccgtta aggacaaatg aagagataca agattttcag ttttggctaa gaagaacaag
     6661 taatccggaa agagatagtt ttttattttt gtttggaatc aataatgggt tacgtatgtc
     6721 agatattata ggattaaaag ttggagatat acgaggcaaa agtaagccta ttattgttga
     6781 acataaaaca ggtaagcgaa aaccgatttt tatagataat ttgagagaag aaattttatt
     6841 atatactgaa ggtaaagaag aaaatgattg gttatttccg agtcggcaac aaggtcgaca
     6901 tataacaagg gatcgtgtct atcagatata tgcagatata gcagaaaaat taggacgaga
     6961 tgatatagga acacatacat taagaaaaac ttttgggtat cactactaca agaaaactag
     7021 agacattgct accttaatgt ttatttttaa tcatagctcg caagcaatta caaaaaggta
     7081 cattggtata actgaagatg aaataggagc atcacttcgt ggatttaaac taggtgtata
     7141 atttaaaaat ttttataaac agtaaagaca aagattaaaa gctattttta tctagaacga
     7201 ttttatttca taaagttcgc ggaataaaag ctggggttac tggtacttgg tataggttcg
     7261 tgttatataa actaagtaat taaataacat ttttaggttg gaaaaggatg aaattaaaaa
     7321 aagagaatca atacggtctc aaaaacgcaa acgctgcgga ataagaaaaa tactgataac
     7381 tcaacgttta gcagactatc taatagggat agtctgcttt tttcgttttt gaccatactt
     7441 ttgaccttct gcaaacttcc ttgcttttcc tctgtagaac tcatgaaatc tgcaaaacga
     7501 attgccgttt cctctgtctg ctttttcgcg atatgggtgt atatatcatc catagtggtt
     7561 ttaatatctg tatgtccaag tcgaacatac acttctttga cagacgcacc agattcaaag
     7621 agcagactac aatgggtatg acgaaaacca tgaagtgtga tatgttttaa gttattctct
     7681 ttaatgatga ttttcaaatt gtgatttaca tagtctaaat aaaggggctt attttcttct
     7741 gatgaaaaca aaagttgttc accatctttt ataggtttga ttctcatttt tagatattat
     7801 gcccgcacgt attcatcaca tttctattgt gaatcgcttt attcgaccga catttgactt
     7861 ttatcataat atactcggtt taaaattact aatgaaaaca attaatcaag acgatcatac
     7921 catgtatcac ctgttttttt cagataatca ccaccgtgtg ggaactgaac tgactttttt
     7981 tgaagtacaa gaagggaaca accagttttt tggaaccaat acgattgaac gaacgatact
     8041 aaaagttcct tccgaagcat cattgcattt ttgggaaata tattttgaaa cacaaggcgt
     8101 ttgccattat ggaatcgaat cattttctgg acgacctatc ttacgttttg aagggccaga
     8161 tgcaacacga ttagctttag ttcccttacg cgaatttgaa gatattgatg attatttccc
     8221 gtatgtcaca gaccagatcc ctacagagca tgccatttta ggaattgatg ccattcaact
     8281 acgcgtacaa tattccgatg cgactgaacg agagttattg tcggtaggct ggtagcacaa
     8341 agaatacgtc agtttctttt cgggtcatca acgagtgaca atcttagaaa atcaagacac
     8401 tcagttctat caagaagttc agattattca agatcgtgag aatccagttg ccattgaagg
     8461 gattggtagc gtccatcatg ttgcgtttgg cgtagaaaat aaatctgatt tacaacgcat
     8521 cgacaaacaa ttacaagagc gcaattttat aaattcagga ataaaagatc gtgaattttt
     8581 tgtatcactt tattatcgtg acccaaatca attattgatt gaaattgcta caggggaagg
     8641 aaacctagat gcaaaagcgt atgaaaatca aagtccacgc tttgaagaaa ttcctttatt
     8701 tttaccgcag tacttaaatt tcattcgtga acaagtggag caataattag ctaatcaaat
     8761 ccatgacgaa gagtaagagg ttgactcata agtcaaaaaa taaagaaaaa cgcatcaaat
     8821 ccattctaaa gaatgttgat ttgatgcggt tcctattttt ataaactatc attttcgact
     8881 aataaaacta cttatgagtc agcctctctt tttttcttga tttttatacc tctttctagg
     8941 taaactaaac atctgccaaa aataccgtgt ctcattgaaa cattctcaac tatttcaaaa
     9001 taaaggggag taccaaatga acgagtatat tgaaattatc cacgcaagag aaacaactta
     9061 aaaatgtcag tctcaaaatt cccaaaaata aaattagtat ttttaccagc gtatctggtt
     9121 caggaaaatc atcgattgtt tttgatacta ttacccaaga agctggtcgt caactgaatg
     9181 acacctatga tagttttacc cgtttatttt tacctaaata cagtcgtcca gaggttgatg
     9241 agattgataa tctttctacg gcgattgtgg tcaatcaaaa gaagattggt ggcagtgcgc
     9301 gctcaacgtt agggacgatt actaacatca atgcgttatt tcgtgtgttg ttttcatgtt
     9361 ttgcaacacc tagtttagga tatgcaaatg cttattcatt taatgatcct agtggaatgt
     9421 gttctgtctg tcagggaatc ggtaagacga ttgcattttt aataggattg ctttattttt
     9481 cggctattga taatgctgat tttgtatttt ctgatccatt taccgcagaa gttttgtctg
     9541 gaattgagga acaggcaaag aatcatggtt attttgtatt ggtgcataat gttaattcag
     9601 cagaagatgt taaaactatt cagagaaatt ggcgttttga ggggttcatt gttgttggtg
     9661 tacgtgtaaa agaatttttg gaattaaata acgtttttga tggtcctgtt gtttatattg
     9721 atacgcatct ttcaaaagag acattaagag aacttgaaca acaaatgaat cgtttatttg
     9781 taaatacaaa tgattaccaa gctagtacat tggcagtgga acatttgatt cattatggtc
     9841 atgagaaaat agcgtttctt tcttttgact acattgtagg tgaaccaagt gttattcaag
     9901 aacgacatca agcttatatc gatacattaa agaaatatca tttaaccttt aaatcgcatt
     9961 ggatatataa caactctgaa tttgagcgaa tttatcaaga attagctgaa tttactgcaa
    10021 ttgttgttac tggagattat ttagcggcta aattagtaaa atttttaaaa aataagaata
    10081 aacgagtgat ggaacaatta tcgattattg gatttgatga tattactttt gcagaattaa
    10141 tggatcctgc gttgacaaca atacgactaa atccaggtaa caagggggcg attgcttttc
    10201 accaactgat tggactggcc aaacaagaag aaataaaaaa tcaagtacta ctattagagg
    10261 gagaattgat ttctcgagac tctgttcaca aaatcaaata aaaaagctct gtgaaataag
    10321 tatagttaac aataaaatat ccgaacgata gagagaatga actgtgatct agcagttcct
    10381 cgtcgcaaat tcactagaat tagcattatt tactctaaat tgttcggata ttttttcttg
    10441 tttttaatta tgattacagt gcctattttt aacgaaattt gcttattttc aaaatgtatg
    10501 attatatgtt agtaaacact ttcaaatatg aaaagaggag ctattatggt aggaattagg
    10561 gatgttgcaa aacgggcggg tgtgtccatt gcaacagtat cgcgcgtgct aaatgaggat
    10621 ccgacacttt cagtaaccga tgaaaccaaa cgaaagattg cagaatcggt taatttttat
    10681 cattatgtca aaaaaagtcc cgttgcaaaa aatttaacaa atattgcatt aataacgaca
    10741 gtttctgaag tagatgagtt ggaagatccc tattttcgaa cgattcgcag agggattcaa
    10801 gtcgaagcag aacaaagcaa agtcaatttg aagaaaatta ttcgtttgtc agaaacaccg
    10861 ttggagcaag acgatttgct aaaatgtcaa ggtgtgttga ttattgggca ggtgctatct
    10921 tctgtgattg aagaagtatc agcaattaat tcgaatgtcg tagtgattga tgaccctagt
    10981 attgaagctg aagtagatgc agtttatact gatttaacaa aagcaacgaa tcagcattta
    11041 gatcgattgt ataaaaaagg acaccgaaac attgcgttta tcggaggagg acgtgttcgt
    11101 ttagatagcc aaggaaataa acacgtgtca gatgatgatt atcgaaaaat agcctatgag
    11161 acgtggatga aacaaaaagg attagaaaaa tatagtcaaa tttatttaga aggctggacc
    11221 acacttgacg gaatgcaggc agcagattta cttttgaaaa aacatgctaa agataagttg
    11281 ccgactgcta tcatggtagg aaatgatcct attgctgtgg gggtttatcg ttcacttcaa
    11341 aaaaatggtc tacatattcc agaagatatt tcgattgtga gttttgataa tattgaagtt
    11401 gctgaatttt taacacctgc attaaatacg gtgaatgtga atacagaaga aattggaaaa
    11461 ctagcagttc gcatggttaa tgaacgaata aaagaagtta gaaatgtttc gatcaaagtg
    11521 attgtttcta ataatattat tgttagagaa agtgaaaaaa atatcgatta aaaaagtaaa
    11581 catccaaaaa agggtgttta cttttttgtt tactaatatt tttagtaaaa aatctcgaca
    11641 aaacattaag taaacagtta tggtgttgtt aacaaaaaga aaacgcattc aaaacaggag
    11701 gaattaagat gaaacatttc aaaaaaatag ctttaacagg gatagctgtt ttaactggga
    11761 cattactcgc agcatgtggt gggggagata gtaattctaa agacgggcaa gtaactttaa
    11821 gctatgcgag ttgagatact gaccaagctc caggactaag aaaaatgtta gatgaatttg
    11881 aaaaacaaaa tcctgatatc aaagtagaaa tggagacaac gccttgggat caatattggg
    11941 ttaaattaga agctgcaaca acgggaggta atatgccaga tgtagtaacc atgcactcta
    12001 gcgaatccta taaatatatg tcacagaatg ctttgatggg acttgatgat gttatcaaag
    12061 aaaatgatat tgacatggga aactttactg aaggaattgc tgatttctat acgcttgaag
    12121 acgaactata cgcaatccct aaagatgctt ctgtagtggg gctatggtac aacaaagaat
    12181 tatttgataa tgcaggtgtc gattatccag atgaaacatg gacatgggaa acactcagac
    12241 aagctgcaat tgatttaaca gatgaatcaa aaggcatcta tggttttgca tcacatattg
    12301 gaacagagac tggctactgg ccatttattt atcaaaatgg gggagaagta tttgccgatg
    12361 acaatacaaa atcaggtttg aataccccag aagcacaaga agctttagct ttttatcgag
    12421 atattgcact tgttgatcat gcttcaccaa caattacaga attacaagat cctgataagt
    12481 taagtcgttt tcaatcaggt agattagcaa tgatgattga aggaaactgg tttgcaccgg
    12541 gatttttgaa aaatgactat ctagttgaaa atgttgcttc tgccgtgtta cctaagggaa
    12601 aagttaatgc aacaattaca aatggacttg gttgggctgc cagtgcgagt acaaaatacc
    12661 cagaagaagt gaaaaaacta atggcatttt tagctagtga ggacgccaat aaaatccaag
    12721 cggaaacggg ggcatcaatc ccagcactaa aaggctttga agacgagtgg gctgcttctt
    12781 cagatacaat tgatttgagt gcttttggaa aaatgttgga gtatggagtc cctcgtccat
    12841 acaataaaga tggattacga gctgaacaag cagaagttca aattattaac cgtattctgt
    12901 ctggtgaagt aactgttgaa gatggaaccg ctcaaattga aaaagaagtc aatgcgatat
    12961 tgagcggaaa ttaaattagt taattataca atatacaggc gaaaggattc tatttcgtct
    13021 gtatattaat ggatgaatac caaaaagggg gatattatga aaaatcgaat ttttggaaaa
    13081 tatcgtaaag atcaagtgat atggggctat atttttattt ttccaacggc attaggatta
    13141 attttattaa atatatggcc agcaattcaa acagcaattt taagttttca aaaaacggta
    13201 gggttcggtg atactgaatg ggtcggatta agaaattacc aaaaaatgtt tgcagacggt
    13261 gaagtttttc agtctttatt aaacacatta atttatgcag tggtttcgat acctagtatt
    13321 gttgtattat ccttgttggt ggcagtttta atgaatagaa aagttaaagg attgtcgatt
    13381 tatcggacaa tttattttct accagtcgtt gctgcaccat ccgcagtcgc aatgatttgg
    13441 cgctggatgt tcaataatga ttatggactg attaataaca tgttggctaa aataggtctc
    13501 gatggaccag catggttaac ggatccatcg attgcgattt attctattat cattgtagga
    13561 gtttggagtg ctattggtta taacatggtg ttattactag ccggcttata agaaattcct
    13621 aaagattatt atgaagcagc ggaaattgat ggtgctagtc caatccagca gtttttcaac
    13681 attacgttac cattagttac accaacatta ttttttgtag ttgtaacaac agttatcaat
    13741 gctttccaag tatttgatgt tatttttatg atgattactc ctaatagtac cgccatgttt
    13801 aaatcacaat cattagccta tttattctat aaacattcat ttattttaaa tgacaaggga
    13861 tatggttcgg caatcgttat gttcctttta gtgattatat taattattac tgcaattcaa
    13921 atgaagttgc agaaaaagtg ggtaaactat taaggaggct atcaaattat gaaaaataaa
    13981 tttttttcaa atgcgccaat ccatatcatg cttattttag gtggtattgc aatggcatta
    14041 ccttttattt ggatgatttt aacttcaggg aaaacgttgg ttgaatctac ccaaattcca
    14101 cctacaattt tccctgagaa atttcagcta agtaactatc aacaagtact tgatttatta
    14161 ccattcatgg ctttttatat caatacattt ttaatggttg ttggacgagt gattggctcg
    14221 gttttatttt cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc agggaaaaat
    14281 atccttttca gcttagtact tattcaaatg atgattcccg gttccatttt tattactcca
    14341 cagtatttat tagcacaaaa attgggtatt ttaaatacaa ttccagcatt aatattacca
    14401 ggggtagtta gtgcgtttgg tacgttttta ttaagacaat ttttcttagg tttacctgat
    14461 gaattggagg aagcggccta tttagaaggt gccaatattt ggacgatctt tttcaaaatt
    14521 atgttgccgc ttgctaaatc aggattggtt gcacttggaa tattcacagc gttgtttgca
    14581 tttaaagatt taatgtggcc attgattgtc aatacttcaa ttgataagat gcctttagct
    14641 ccgggattag caaacttaca aggacaatat gccacgaact tcccacaatt aatggcaggt
    14701 tcgttattag caatttggcc aatagttgtg atattcatcg ttttccaaag aaaattcatt
    14761 gaaggaattg ctacaacagg tagtaaatta taaaactatt aattagtgat tggagagtaa
    14821 gtacatgaca aaaattacat ttattggtgc aggaagtact atttttgcaa aaaacgtatt
    14881 aggcgatgcg atgttaactc caagtctaca agatgcacat attgcattgt acgatattga
    14941 tgaaaaacga ctaaaagaat caaatttaat gcttaaaaat attaataaaa attcgaatga
    15001 aaatcgtgct gatattgttt cttattatga tcgcaaagaa gcgttaagag aggctgattt
    15061 tgttatcaat gctattcaag tgggtggtta taaaccttca acagtgattg attttgatat
    15121 tcctaaaaaa tatgggttgc gacaaactat tggtgacacc atggggattg gtgggatttt
    15181 tagaacccta cggacactac cagtaatgtt agactttgct aaagaaatgg aagaagtttg
    15241 tccaaatgct tggttattga actatacaaa tccgatggcg atgttaacca tgggtgtttt
    15301 aaaagcgacg agtattaaga ctgttggttt atgtcatagc gttcaagtat gcgtaccaga
    15361 attgtttgag catttaggaa tagccgatca atatgactta aatgactttc aatggaaaat
    15421 tgcaggcatt aatcatatgg cattcttgtt ggaaattaca aaaaatgggg aagatttcta
    15481 tccaacaatt cgtcagttag ctgcagaaaa accaaatcca catccagatt ctgtacgttt
    15541 tgaattgttg aaacgttttg gttactatat tacagaatca agtgaacaca atgcagaata
    15601 ccatccatac tttattaaat caaaataccc agaattgatt gacgaattag ggattccgtt
    15661 ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca tgcgagagga
    15721 cattgtaaat aatggggagt taacacatac gagaagccgt gaatatgctt catatatcat
    15781 ggattctatt acgacaggaa ctccgtcagt gattgcagga aatgtcttaa ataaaggctt
    15841 aatcacaaac ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg ataaaaatgg
    15901 cgtgcaacca acctatgtcg gtgaattgcc aacacaatta gctgctttaa atcggacgaa
    15961 tatcaatgtt caagaactga cagtagaagc cgctttaaca ctagataaat caaaaattta
    16021 tcaagcagcg tatttagatc cacatctatc atctgaattg tctttaacag atatcaaaaa
    16081 tttagtggat gatttaatcg aagcacatgg ggactattta cctgaatata attaaaaaga
    16141 agctgaaaga gtaaggaatt ttcaattcct tactcttttc ttagttaaat ttagtaaaaa
    16201 tattgacaaa acacttagta aacagttaca ttagtgttaa caaaaagaaa acgcattcaa
    16261 attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag cttgtatagc
    16321 actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg caagcggtaa
    16381 ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc aggagattgc
    16441 taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg taccgaatgc
    16501 tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca ttaatcttta
    16561 tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg acttaaccga
    16621 agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta ttgaggatcg
    16681 tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca aaactgcttt
    16741 tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa tagtagctga
    16801 gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct ggactttaaa
    16861 tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag cgaacaatgt
    16921 ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc aaaaagattt
    16981 taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc aaggtgccgg
    17041 ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc cgaatggttc
    17101 atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa cttttccttt
    17161 tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag ccttatcagt
    17221 ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata tgacaacacc
    17281 tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg agggcgttat
    17341 cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta ctgatcgtca
    17401 tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct taacaacaaa
    17461 ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt tctttaatcc
    17521 aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt taaatcgtta
    17581 ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt tctactttcc
    17641 aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt ataattttga
    17701 ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta tcaaatcgat
    17761 tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt ttggtatttt
    17821 gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag catggttctt
    17881 ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa tttttaacta
    17941 tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca gtcttttagg
    18001 aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag gaattgccat
    18061 gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta ttgaagctgc
    18121 tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca cctacttatt
    18181 accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag catttgatca
    18241 aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg ggttacttgt
    18301 ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg ctttagtctt
    18361 atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa aatttgaaat
    18421 ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata cttgctttta
    18481 ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg ttcatttaaa
    18541 acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac tttggaaaat
    18601 ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc aatcatcaca
    18661 atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc actagcaaga
    18721 aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt aggaattttt
    18781 gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat tggtttagca
    18841 aatatgtggg gattaatctt actctatctc acttatgccg taccacagac actattttta
    18901 tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc tgaaattgat
    18961 ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa accaatgcat
    19021 gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct accattatta
    19081 attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa ttatacggga
    19141 caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat tgtcacgatt
    19201 acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa tggagcagtg
    19261 aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat tatatttgac
    19321 aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt aggaatcgaa
    19381 gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta ttctggtggg
    19441 tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc gcaagatcgt
    19501 ttattatctt tagacacatt accattagaa tttccaggat atggacatgg tgattttaga
    19561 gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt tcgttatgat
    19621 agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt atatacggaa
    19681 gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag tggattgact
    19741 ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc tacgcaatta
    19801 gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc aattgatttt
    19861 ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga acgtcaaatg
    19921 actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg atcaagcagc
    19981 catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt tcaaggagag
    20041 gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca aaaagatcca
    20101 tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg gcaactaaaa
    20161 actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga aggtctgaat
    20221 acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg aaactatcgt
    20281 ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga ttttaacgaa
    20341 gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat gtttgtttta
    20401 gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga ttgggttgaa
    20461 acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca tgataacggg
    20521 atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga actatttcga
    20581 gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag tcgaaatcaa
    20641 tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca aatgacggcg
    20701 atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa tatgacagaa
    20761 gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg ttatatgtta
    20821 gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt atttgaaagt
    20881 tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc acaaacttgg
    20941 acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac cagcttagtg
    21001 tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca aacaggaaga
    21061 gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg ttatgagcta
    21121 gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt tgccttttat
    21181 aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag tccgtttgaa
    21241 ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat ggtcttttat
    21301 ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc aggattagca
    21361 gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcgatgaatt gatgaatctc
    21421 ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt ttatttgaaa
    21481 cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact cattccaaac
    21541 gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt gaattgggaa
    21601 aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga taaaggacta
    21661 ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta tggcatcttt
    21721 gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa tcagtcgggc
    21781 attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa tagtatgacg
    21841 gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga agatggctat
    21901 tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac tggtgaatgg
    21961 tttaaagata gccaatggac atggacggat atgcgtacca atatttttac gaatcaatta
    22021 gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca accacgttca
    22081 attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat gttggcgacg
    22141 ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact aggaatgacc
    22201 aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc tcagtataaa
    22261 cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa taaacgaagt
    22321 cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag attctcaact
    22381 tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc agaagctcag
    22441 ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt aagaaaaaat
    22501 agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga taaaaatgaa
    22561 catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat cttaaacgtt
    22621 acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt aatcaataat
    22681 tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt tgaaagtatt
    22741 gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg ctcatcacct
    22801 atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac aaacatttac
    22861 aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt gatcgaggct
    22921 ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa gatgtggaag
    22981 cattaggtga agattattat ttgatgtttg attttatgat taatcacatt tctcgtgaat
    23041 caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa gaaatgttta
    23101 tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt gatttaatct
    23161 acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt acgacagaag
    23221 aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa gaagttgtga
    23281 aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct ttaattcgat
    23341 tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc gtggaaccag
    23401 atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac gtcgaattat
    23461 taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat gattattata
    23521 tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga aaatcagaac
    23581 gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg gatacgcatg
    23641 atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg gattacacct
    23701 ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt gccaattaca
    23761 ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc gataatgacc
    23821 gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca caaatttatt
    23881 acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg aaagaagggc
    23941 gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg caacggccag
    24001 tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg tttgatttgg
    24061 atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca cgtaaaaata
    24121 aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa tttatcattg
    24181 agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct attccataga
    24241 acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa aaaggactca
    24301 tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc cataaaatta
    24361 ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta tcgtaaaaaa
    24421 ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga aaaagaaagt
    24481 ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac cgatgtgtca
    24541 tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa agtgttagaa
    24601 gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac caagcgaaat
    24661 catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca taagtgggcg
    24721 actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta tatggacgat
    24781 actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga tctcaatcaa
    24841 ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg gtattaggac
    24901 tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg gattaaaaaa
    24961 aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga aattctacga
    25021 gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg actagtcact
    25081 gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga cgtctcttca
    25141 aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt caagcacatc
    25201 aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt gcagaatcaa
    25261 ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga gacgaaaacg
    25321 gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca gccctttgaa
    25381 tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg ttacttttgg
    25441 ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg cgtcagcaca
    25501 gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa tgttttatgc
    25561 aacactagtg atatttttta gaattagatt tctaatgtgc attttttgag tattaaggag
    25621 caagtataca aagatttata aagctgatta aatttgaata aaaatatgca ggaacgctac
    25681 tatttttagt agtgttcttt tttatatttg aataaaaaat gtggtggtta aatggtggtt
    25741 ataacaaaaa atgaattaat gaaattaggt tatgggaagt atcagtctga gagaatcata
    25801 cgacaggcaa aagcaattat ggtttcaaaa agctatacct attacgaaaa tagaagatta
    25861 ggactagtac ctgttgaatc tgtagaagaa gttttaggta tgaagataga ggtagaggag
    25921 gattttcaga atcatggtta attttaaaaa agataaaaat gggaaatatc attttactgc
    25981 tagtttggga ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag aggggttaaa
    26041 acagttaaag aagaaaaaaa gcgtataccg aacttgtttc taattatgga aaagaagcag
    26101 aaacttctca aagtactatg ttgtttgagg agttttttta taaattattc ctaccttatt
    26161 atcaagggaa agtagaagaa agaacatata aaaatcggtt atctacaatc aaaaaaactt
    26221 ttcttatttc tttaaaatgg agttaagaga aatacgtcca attgacattc aaaagtggca
    26281 aaataattta ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg ggatttttca
    26341 gatgtcatta gatcgagcag ttgttttggg actacttgaa aaaaatcctg caaaagttat
    26401 tggaaatgtg aagaaaagga aaactacagt tgatttttgg acaaaagaag aatttacaaa
    26461 agttatttca actttttata ttgatgattt gtaccaacat tttggtttta tttgtatgtg
    26521 gttacttttt atgaccgtta tgcgtttggg agaagccact gcattaactt ggaaagatat
    26581 tgattttgaa aataaaaact tgcgtgtgaa taaaacactc tattataaga gagccaaaac
    26641 ctatgagttt gtagaacccg aaacggcttc tagtattcgt acaatcgaac tagacgacaa
    26701 tacaattacc tttttaaaag cttggaagga gcgtcaagtt tcaataggag gcatgtcgtt
    26761 tgtactttct tataatggaa cacctactca gaaatataca atccgtcata ttatgagaag
    26821 acatgcaacg atcgttggtg ttcatataat ccgcatacac ggattaaggc atagtcatgc
    26881 ttctttgctt atatcacaat aaaaagatgc acttcgaatt aaagaaaggt taaaacatgc
    26941 tgatgtacaa acgactttag gaacttatgg tcatttgtat ctaaattcat tgagagagat
    27001 aacaaatgat cccacagatt atatccaaac taatttttct aaaatcaaac cgacacctat
    27061 ttacgataat aaaaaataaa tgataaacaa aagtaggatc acaccaaaag aaacacacta
    27121 gaaaaactat aatacaggct tttctagcgt gttgtttctt atttccgcct atttatacat
    27181 tatactcata aagcatttaa tgcatcgatc gtagccgcat tgtctatatg ggtatataaa
    27241 gttgttgtat tggtacttga atgtcccaat tgttgggcag ttaataccaa tgaatttgtc
    27301 tgttggtaga gtctagtagc taatgtatga cgcagtttgt gagggctgac ttgaattcta
    27361 aagccttttg aatattttgc taccatccgt tcgattgcaa taccggatat acgcttggct
    27421 tctccttggt aaattgctag aaataaagct ttttcgtttt ctgttacttt atatcgttct
    27481 ttccgaatag ctagataatt agctaaatat tccattgcga atgctgctat attgacagca
    27541 tctcttttat ttccttttct agttactacc acattattct tatctaaaga taggtcatct
    27601 aaattaatat ttataacctc ggagagtctc aatccagatc ctaaaatcag tgcattgatc
    27661 gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttact caaagtattt
    27721 ttatattcat tggcaatata atctagataa ttttgtgtat ccttatctag aaataatttt
    27781 ttctcaatgg cattggcacg tgcagctaac gtagttttat ctgtgatggt tgctacttga
    27841 tccataggat tattgtctaa gtatggtttt cctagaggat tattggaact tttcgaaaga
    27901 tatttaaata gaacctttaa tgccgtgata tttctattga tagtagaagt agatagacct
    27961 aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt ttcttttgtg
    28021 cgacgtttca aatgagattt gtacaagttt aggtcatagg cagttaactg tcttatcgag
    28081 gtgatagaag tatcaggaac gatagactca ctcagaagcc attcataaaa gagcttataa
    28141 ttttttagat attctaaagt ggttcgaggt gattgatctt tatcctcacg atcttgaaca
    28201 aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg atatatttct
    28261 gtatttttca tgtcattcat cctttcaata agtaggggag aagagtatct ctataattgg
    28321 actataccaa ttatagaatg ttttgtcaat tggtatgctc taaaatttat taatttgttt
    28381 caaaaaaagc atataaacag gtatacgttt aataatttaa aataaatggt atacataaat
    28441 agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt aattatatag
    28501 aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt taaaatcaac
    28561 aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca attaaagaaa
    28621 gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag tttcttaaag
    28681 aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt cgagcggcaa
    28741 ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa gcattaactg
    28801 aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc tagaagccaa
    28861 agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg aaccccttgg
    28921 tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct cagcttttgg
    28981 tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac aattgataaa
    29041 gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt ttgtcttttt
    29101 gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt tagcggtata
    29161 tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca acactataaa
    29221 gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt tattagctgc
    29281 tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa ctatatagaa
    29341 aacgtaaatt ttctatatag ttggattaag ataacgacta tttattttat catattttta
    29401 ctatacccta tacttttact tggtatattt atgaataatc gcaaatttac ccctatacta
    29461 ctagtttgta agttagatta ataaaaatac taaagttatt tgttctttta ttaaatacac
    29521 ctacaactta taaattgatg gtatttagat aacacgaaag aataaaaata acagctgata
    29581 gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat gaacttgctt
    29641 ctgaaaactt atgaaatgaa agacctttta catcttgcag aaatcggtac tgggattaaa
    29701 ctaagcaata aaaactaatg ttcccctcat tctttgaact tctactattt gatttattta
    29761 ttctgataaa tgacgatttc accttcagga tcttcaaatc ccgtatagaa tttatttaag
    29821 atctcttcag tatcagtaat aggataggcg ataggccaca taccctttga tggttagaat
    29881 aaactgctca aaatctgtat cgtatgttgg ctttagcata ttaacaaagg ctatctccaa
    29941 attataaacc ataagcgttg aatcgttctt aacaatttca attcttccta acccaaagtc
    30001 agaagatata attaccggac ttgccttacc cttcatgtgg gttgttgaga ttccatattt
    30061 aaagggaatt gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa aaaaatcttt
    30121 cccctgctag tctcctaagt ccctttgtag cggatatggc accataatct cattttatcg
    30181 aagtgagtca tggtttatct gttggccaac caaaattgat acaatctaac gatgcaccct
    30241 ctacggcatt ctaggggagt gcacttgcat tcacccaata aaaaccggca gttagcaaag
    30301 aacttatgta gtcgtatgtt cttactgtta acatacaaga ggtctccttt ccacaattgc
    30361 ttagcagtaa acgggaattt acaaagctaa tctcttccat tttcttatct ttgttctaaa
    30421 agtcccaaat ggagcaactg tatttacatg aataaattta tatacttccc atacagccgt
    30481 tttggttgcc tcgtctgccc attttctttt gtgtgcagtg aatagatctt catcactcat
    30541 tgaatcaatc atcatgttaa tatcatcaat attacgattg agaatttcct ctaattcttt
    30601 caatggtaga tgtgcatagg tatttgtaaa ccacttatat aactctccta actgattcca
    30661 tttaaattga tcagaaggcg ttttaacaga taatccattt ctttcatcat tttcccattt
    30721 tagaattaaa ttcgtccaac caacttgata tgctaaattt tcagcaggtg ttctatccac
    30781 ttcctcaact cttttatcct tcaattcttc aggaatagtt tcaaattcta aaatatattt
    30841 agtataagtc ctatttattt ctttttttaa ttcttcttta tttttatatg ttctcattat
    30901 attacctcca taaattccta attgtctcct ttaattcatc tataatttca tccacgatat
    30961 tttaatccgt tctacaatgc atatctgata tgcattgact gtctaactca tatgtgaatg
    31021 atttataaaa tatcccaacg tctcatctaa agaaatgttg atcgtcagca aggagctaaa
    31081 aaatacgaat atatttcttt tgtagtaaaa tcggactggt ttgcatatca ttttcttcgt
    31141 aaatatcaaa atcatgttaa ctactatcaa ttactaataa aaactaccac tttaagcgtt
    31201 aagaactgtt aagtttagtt aagtgaggat agttcacaaa atccattgtt aaacttcacg
    31261 aaagtggtaa aactcaaaat gctttatata ggattacagc atcgctcttt cttcgattgc
    31321 gcgttgggta aaaaatatta tgaagtaaaa attgacgatg acacgttcct tacggctcat
    31381 cagatgaaac agctacaaaa acgaaatgat caactcgaag aaaatttaat tctaaaaaag
    31441 cgattgccat attcacgcct cgctcgacga actattaaag gtcaagattt acctgaagat
    31501 catccttttt aggaaattga agctttttat gttgcacaat gcatttataa tactacaatg
    31561 atgttttcac cagatattct tctagggtgc ggtgtaatga aacagataca cttgcaaaag
    31621 aaaattcacc gcgaatttac taaaattatg aataattatg ttaatcttcc gaaatttttt
    31681 tccaaaatta agggatcaag ctggtgttat agattgttta aggttggcaa aaaaataact
    31741 tctttttaaa caattataga aaaccgatac tctgttaagt tggttgacac ggtgtcgatt
    31801 tttatgactt tcttattctg aaacttcact aacggacagt acctaaaaag tctatagtat
    31861 ggacgtttac gagtactatt ttatcaaaaa tgctgctcat ttcaacttat atgttttagt
    31921 gaactacccc cacttagc
//
LOCUS       DAZKAS010000017        30589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000017 DAZKAS010000000
VERSION     DAZKAS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.017"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            363..731
                     /locus_tag="ACG8H7_000664"
     CDS             363..731
                     /locus_tag="ACG8H7_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP8982781.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="ACG8H7_000665"
     CDS             911..1852
                     /locus_tag="ACG8H7_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP8982782.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="ACG8H7_000666"
     CDS             1921..2652
                     /locus_tag="ACG8H7_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8982783.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="ACG8H7_000667"
     CDS             2796..4976
                     /locus_tag="ACG8H7_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP8982784.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="ACG8H7_000668"
     CDS             4996..5478
                     /locus_tag="ACG8H7_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFP8982785.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="ACG8H7_000669"
     CDS             5494..6255
                     /locus_tag="ACG8H7_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP8982786.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /gene="murC"
                     /locus_tag="ACG8H7_000670"
     CDS             6386..7720
                     /gene="murC"
                     /locus_tag="ACG8H7_000670"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP8982787.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="ACG8H7_000671"
     CDS             8027..8512
                     /locus_tag="ACG8H7_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFP8982788.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="ACG8H7_000672"
     CDS             8505..9188
                     /locus_tag="ACG8H7_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP8982789.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="ACG8H7_000673"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="ACG8H7_000673"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP8982790.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8H7_000674"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8H7_000674"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFP8982791.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8H7_000675"
     CDS             12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8H7_000675"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP8982792.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="ACG8H7_000676"
     CDS             13485..13817
                     /locus_tag="ACG8H7_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP8982793.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="ACG8H7_000677"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="ACG8H7_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP8982794.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="ACG8H7_000678"
     CDS             14643..16016
                     /locus_tag="ACG8H7_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP8982795.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="ACG8H7_000679"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="ACG8H7_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFP8982796.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="ACG8H7_000680"
     CDS             17071..17511
                     /locus_tag="ACG8H7_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP8982797.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="ACG8H7_000681"
     CDS             17655..18356
                     /locus_tag="ACG8H7_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFP8982798.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="ACG8H7_000682"
     CDS             18353..19474
                     /locus_tag="ACG8H7_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP8982799.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="ACG8H7_000683"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="ACG8H7_000683"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP8982800.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="ACG8H7_000684"
     CDS             20853..21761
                     /locus_tag="ACG8H7_000684"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP8982801.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="ACG8H7_000685"
     CDS             21796..22068
                     /locus_tag="ACG8H7_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFP8982802.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="ACG8H7_000686"
     CDS             22079..23125
                     /locus_tag="ACG8H7_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFP8982803.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23376..25985
                     /gene="clpB"
                     /locus_tag="ACG8H7_000687"
     CDS             23376..25985
                     /gene="clpB"
                     /locus_tag="ACG8H7_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP8982804.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26136..26822)
                     /locus_tag="ACG8H7_000688"
     CDS             complement(26136..26822)
                     /locus_tag="ACG8H7_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982805.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26800..27294)
                     /locus_tag="ACG8H7_000689"
     CDS             complement(26800..27294)
                     /locus_tag="ACG8H7_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFP8982806.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27314..28534)
                     /locus_tag="ACG8H7_000690"
     CDS             complement(27314..28534)
                     /locus_tag="ACG8H7_000690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8982807.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28681..30516
                     /gene="lepA"
                     /locus_tag="ACG8H7_000691"
     CDS             28681..30516
                     /gene="lepA"
                     /locus_tag="ACG8H7_000691"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP8982808.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        10250 a         5445 c         6593 g         8301 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt gtttttttaa gatttatata agtcgagaaa aaaagtcaaa aaactatgtg
    23281 tatattttga ccaaaattga ccaaagcgat ataatagttt ttgtaaagat caaagaaata
    23341 agtgatctta cctttaatta ggagggtata cctgtatgaa tattgagaaa atgactacga
    23401 cgcttcaaga agcgattgct gaagctcaac agattgcggt cactcgaaaa catcaagata
    23461 tcgacattgc ccatgtatgg aaaatctttt tacaatctaa tcattttggt cgaaatttct
    23521 atacggatgc cggattggat gtcgaatctt ttgaacatga aattgatcgt ctactggatg
    23581 agtatccagt tgtttctggt ggaaacgtcc aatatggaca aaacctcagc cagaatttct
    23641 ttcgattatt gaatgaagca gaccaaatac gtgaaagctt cggcgacgag tttctttcaa
    23701 cggaagtcgt tattttagcg ttgatgaaat taaaaaatta tccgttaact gtttatttga
    23761 ataaaaacgg attatctgaa aaagaattgc gtaaaaacat cgaagagatg agaggaggag
    23821 aaagagtgac ctcacaaaac caagaagaac aatacaaagc attagaaaaa tacggcgttg
    23881 atttagtaca gcaagtcaaa agcggaaaaa tggatccaat catcggtcgt gatgaagaaa
    23941 tccgtgatgt catccgtatt ctttcacgaa aaacaaaaaa taatcctgtt ctgataggag
    24001 aacccggtgt agggaaaacg gcaatcgttg aagggcttgc tcaacgtatc gtacgcaaag
    24061 atgtgcctga aaatttaaaa gataaaacga ttttttcatt agatatgggt gcattgatcg
    24121 ctggagcaaa attccgtggt gaatttgaag aaagactaaa agcagtcttg aaagaagtca
    24181 aaaaaagtga tggacgtatc ctcttgttca tcgatgaaat ccacaatatc gtaggggcag
    24241 gaaaaacaga aggaagtatg gatgccggaa atctgttgaa accaatgctt gctcgcggtg
    24301 aattacattt gatcggtgcc acaactttag atgagtaccg tcaatatatg gaaaaagaca
    24361 aagcgttgga acgtcgcttt caaaaggtat tagtcaaaga accaacagtg gaagatacga
    24421 tcagtattct gcgtggatta aaagaacgtt ttgagatcca ccatggtgtt aacatccatg
    24481 acaacgcatt agtagcagca gctactttat ctgaccgtta tattactgat cggttcttac
    24541 cggacaaagc aatcgatttg atcgatgaag ccagtgcaac gattcgggta gaaatgaact
    24601 ctatgccaac ggaattagat caagttacta gacgtttgat gcagttggaa atcgaagaag
    24661 ctgcactgaa aaaagaatca gatgatgcaa gtaaaaaacg cttgaaaaat ttgcaagaag
    24721 aattagctga acttcgtgaa gaagcaaatg cgatgaagat gcagtgggaa acagaaaaag
    24781 aagaagtcaa ttctgtttca gcaaaacgtg ctgaaatcga taaagcaaaa cacgaattag
    24841 aagatgcaga aaataactat gatttagaac gcgctgctgt attgcgtcat ggtacgatac
    24901 ctcaattaga aaaagaattg aaggaattag aagctaaagc aaaagacagc gagattaaga
    24961 tggttcaaga gtccgttacc gaaaatgaga ttgctcaagt cgttggcaga ttaacgggta
    25021 tccccgtaac gaaacttgta gaaggtgaac gagaaaaact aattaaattg aatgaaacac
    25081 ttcataaacg agtgattggt caagatgaag cagttgatgc ggtcagtgac gcggtaattc
    25141 gttcacgggc tggattgcaa gacccaaatc gtcctctagg ttctttcctt ttcctaggac
    25201 caactggtgt cggaaaaacc gaattagcga aagcgttagc tgagaacttg tttgactcag
    25261 aagatcatat ggtacggatc gatatgagtg aatatatgga aaaacatgct gtgtctcgtc
    25321 tagtcggagc tcctccaggc tatgtaggtt acgaagaagg tggtcaatta acggaagctg
    25381 tcagacgaaa tccttataca atcgtcttgt tggatgaaat cgaaaaagcg catcccgatg
    25441 tcttcaatat cttgttacaa gtcctagatg acggacgctt gactgattca aaagggcgag
    25501 tagtagattt caaaaatacg gttctgatca tgaccagcaa tatcggttca cagttactgc
    25561 tggaaggcgt gactgctgat ggaacgattc cagaagcagt ggctgaacaa gtcaacacgc
    25621 tattacgtgg aaacttcaaa ccagaattct tgaaccgaat cgacgacacg attttattta
    25681 caccattgag cctagacaac gtgaaaggaa tcgttgacaa aatggtggcg caattggcac
    25741 aacgtctgga acatcaagaa atcctgctga caatcagtga tgaagccaaa acgtggattg
    25801 ctgaaaatgc ttatgaacca gcatatggtg caagaccatt gaaacgattt atcactaagg
    25861 aagtagaaac accactagct aaagaaatcg ttgcaggaca tgttatgcct aaatcaaaag
    25921 taacgattac tttacttgat ggacaacttc atttcaaaac agaagagcta gaagaaatcg
    25981 tctaatcttc cattgagact ataaaagaaa aaaggggtct gacacaacta gtgtcagacc
    26041 tccttttttc aaataaacag tgttccagaa gtagtttctt cgaaaaataa gccgaaattt
    26101 cttgaagctg cccgcttctg tcacaacctc ttttattagg tatttttcac tttttctaat
    26161 ttataacttt caaagtaacg ctcatctttt ttactttgag accatgcaaa caatcccatt
    26221 aaggcagcac ctatcgtatt tgtcagcaag tcgcccatcg tatccatcaa tgctgctcgt
    26281 ccgacaaaca gtgtgccatc agaagcagca aaacgttgaa gattcatgcc taagaactgg
    26341 tcgcataaga attcccagaa ctcccaaaac acgccgcaaa gtccagcaaa cgcaaagccg
    26401 aataagagga acaaccaagg actggttttt gatatctcag catctttcat caagtaacca
    26461 atcaatccat agccaagagc tgtcaggacc attggactca ccgcatgtaa gattttgtcc
    26521 caaaaagaga tgatactgat caaatgcatc cctgtgccta aaaagacaga gatgaacaag
    26581 aaaaaccagt aaaacaaaac gattgctgct ggtattttaa gtttgaaaac tttcaaaatg
    26641 acttgtggca tgaaaatcaa tgcaatccct aataaagatt ccacgataaa tgtgattcct
    26701 tgagaagtcg aatatttttg tgtgataaat tcataaaaat tatagcagac aacgaataca
    26761 ccaaaagcaa gtaaagctat ttgcaccttt ttgatttctt taatcactga aagtccctcc
    26821 atctaaaaat ctttttactt ctgaaagcaa ctcaatttct gaagaaacca cttttccgtt
    26881 caatttgatc aaacctattg tatataagtt caggtagtga aattgatttt cagctgtttc
    26941 ttgtagtgca gcgatttttt tcgggtgggt tccgccttgc tgtcgagtat cagtatataa
    27001 gccgatcact gggatgcctt tcgcataagc cacaccgatt tccgatgcta ccccggcatc
    27061 gatcgtcagc ccatctagta aagcaatcat taaatcacta gccaacactt tttctgtgtc
    27121 agcaagagca atcattcgac tgtctgcata agcagttttg tcattgattg cttcattttc
    27181 ttgaggaaga tagatcgaaa gctcagaaga gagctctctg atttctttaa ctaactgttg
    27241 gttatataac aaatcacttt tagcaaacat tggtgcggca aaataaattt tcatctaaac
    27301 atcatccttt ttatcaaaaa taatcgctaa agtaagcttc catgtttgga atgatacgct
    27361 ctagtgactt cagtgtttct tcatctgttt ctaaccgttc gattcttgcc aaataagaag
    27421 cacggcgata attcatcatc aagcaagtca atgtttggat atccagtcga acggattttc
    27481 ctataggttc atttaagaca cggacttgtc cctgttcatc ccatgttaaa gcgaagacac
    27541 cattattcca aggagcgaca ggatcttcaa tgatgaaatg aaaagcatca gctgttccaa
    27601 caaacggaaa ccgttgaagg aattctttta catcaacgat ccgagccata aaatatggtt
    27661 cgatctgctc tttgatctga ctatcttcaa tcaaaaatgc taaaggctca tttttataaa
    27721 tatctccatg tacccagtag atcatggaaa aatgtgcgga aataaagttc cataagccat
    27781 ttcgagcttc ttgatcaaga tagaacattt cttttacgtg aaatatttct tcagctaccc
    27841 agtagaacag aacacctctt ggttcatggt tgctatcgta gtagacagcg gctgtgcgct
    27901 catcttcatt ttcgaaacgc caatactctt cccaatgaaa atcactgcgg aataaagcac
    27961 catggttttg ttgggcaaat ttgtcataaa cggaaaatac gtctggatga tcaactggta
    28021 atcgctcaac cataccactt actgggattt gtttaggtag ctgtgtatct ctgattttaa
    28081 aggaaagttt atcagacatg atttcccatc ctttacgccg atagtagggg atactgtaag
    28141 gatacaaata agagatccat tgcttattct tgcgcatatt atccaatgca acgataatta
    28201 gatcttgcat caatccatgg ttagcatatt ctggataagt tccaactcca gtcacaccac
    28261 ccattttata acgtgcaccg tgaatattga cttcacaagg atagatagcg atttgagaga
    28321 tcagtttatt ttcgtggaac catccgaaca ctttcgatac ttccaaaatc ggttttttgg
    28381 aacggataaa agctcgttta ttctcaaacc cgctttcctc gatgtctgct tcagtcactt
    28441 ggaacacata ggaaagcaat tcattgaatt gatcgatatg ttcttgagtg accggtttta
    28501 attcaagatt ttctctaaaa tctttttgat ccattttttc atctccattt attagcttga
    28561 taacagtata gcaaaattct acaaaaaaaa cttctatagc tgactaatta aaaactcttt
    28621 aaattactgc ttttcgttgg tataatggaa agtgctgatt atatgaaaag aggagacgct
    28681 atggatataa agaaaatgaa agaacgccaa gagcagattc gtaatttttc gatcatcgct
    28741 catatcgacc atgggaaatc tacattggcc gatcgtattt tagaaatgac gcacactgtg
    28801 acgtctcgcg aaatgcaaga tcagctgtta gactcgatgg atttggaacg tgaacgtgga
    28861 atcacgatta aattaaatgc tgtagaactc aattacacag cgaaaaatgg agaaacatat
    28921 atctttcact tgattgacac cccagggcac gtcgacttca cctatgaagt ctctcgaagc
    28981 cttgctgcct gtgagggtgc cgttttagtg gtcgatgcag cacaaggaat cgaagcacaa
    29041 acattggcca atgtttattt agcgttagac aatgatttgg agatcctgcc agtcatcaat
    29101 aaaatcgacc tacccgcagc agatcccgaa cgtgtccgtc aagaaatcga agacgtaatc
    29161 ggaatcgatg ctagtgaagc tgtattagcc agtgccaaat cagggattgg gattgaagat
    29221 attctcgagc agatcgtgga atatgttcct gcaccaagtg gagacttaga tgcaccgcta
    29281 aaagctttga tctttgactc tatttatgat agttaccgcg gtgttgttct aaatgtccgg
    29341 atcacggacg gtatggtcaa acccggagac aaaatcaaac tgatgagtaa tggcaaaaca
    29401 tttgatgtta cagaggtcgg ggtcttctcg ccaaaagcag ttgctagaga ctttttgatg
    29461 gtcggtgatg tcggttatat cacagcaagt atcaaaactg tccaagatac acgagtaggg
    29521 gatacagtaa cgttagcaga caatcctgcc gctgaagctt taccaggtta ccgaaaaatg
    29581 aatccgatgg tttattgtgg tctttatcca attgatactt ctcgttacaa cgatttgcgg
    29641 gaagctttag aaaaactgca attgaatgat gctgctttac aattcgaacc agaaacctct
    29701 caagcattag gttttgggtt ccgctgtgga ttcctcggac ttcttcatat ggatgtcgtc
    29761 caagaacgct tggaacggga attcaatcta gaattgatca cgactgcacc atccgttatt
    29821 taccatgtta ataaaacgga tggtacgact gctacagtag ataacccggc agattttcct
    29881 gaacctgtaa cgattcagga tgtagaagaa ccattcgtca aagcacagat catggtccca
    29941 aatgattttg tcggtgcagt aatggaactt tctcaacgaa aacggggaga attcattacg
    30001 atggattatt tagatgatta ccgagtaaat gtcgtctata atattccgct ttctgaaatt
    30061 gtctttgact ttttcgacaa attgaaatca agcacgaaag gctatgcctc attggattat
    30121 gaaatgtctg gttatcaaaa aagcaagtta gtgaagatgg acatcttgct gaatggagaa
    30181 aaagtcgatg cattaagctt tatcgttcac cgagactttg cctatgaacg tggaaaagca
    30241 atcgttgaaa aactgaaaaa actaatccca cgccaacaat ttgaggtgcc aatccaagca
    30301 gctattggac aaaaaatcgt tgcccgttca gacatcaaag ccttgcgtaa aaacgtcttg
    30361 gctaaatgtt atggtggaga tgtttctcgt aaacgtaaac tcttagagaa acaaaaagaa
    30421 gggaagaagc ggatgaaaca aatcggctcc gtcgaagttc cgcaagaagc cttcatggcc
    30481 gttctgaaaa tggacgagga cgaaccgaag aaatagcaag ggtttgtttg aaaacagtgt
    30541 aaaaaataaa ccatgtttac atggatttgc caccggtttg ccaccggtg
//
LOCUS       DAZKAS010000018        30497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000018 DAZKAS010000000
VERSION     DAZKAS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.018"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..135)
                     /locus_tag="ACG8H7_000692"
     CDS             complement(<1..135)
                     /locus_tag="ACG8H7_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP8982809.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     L"
     gene            complement(137..334)
                     /locus_tag="ACG8H7_000693"
     CDS             complement(137..334)
                     /locus_tag="ACG8H7_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP8982810.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(728..2455)
                     /locus_tag="ACG8H7_000694"
     CDS             complement(728..2455)
                     /locus_tag="ACG8H7_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFP8982811.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2448..3641)
                     /locus_tag="ACG8H7_000695"
     CDS             complement(2448..3641)
                     /locus_tag="ACG8H7_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8982812.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3828..4472)
                     /locus_tag="ACG8H7_000696"
     CDS             complement(3828..4472)
                     /locus_tag="ACG8H7_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8982813.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4566..4700)
                     /locus_tag="ACG8H7_000697"
     CDS             complement(4566..4700)
                     /locus_tag="ACG8H7_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFP8982814.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4702..6834)
                     /gene="feoB"
                     /locus_tag="ACG8H7_000698"
     CDS             complement(4702..6834)
                     /gene="feoB"
                     /locus_tag="ACG8H7_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFP8982815.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6831..7304)
                     /locus_tag="ACG8H7_000699"
     CDS             complement(6831..7304)
                     /locus_tag="ACG8H7_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFP8982816.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7705..7929
                     /gene="nrdH"
                     /locus_tag="ACG8H7_000700"
     CDS             7705..7929
                     /gene="nrdH"
                     /locus_tag="ACG8H7_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP8982817.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8088..10247
                     /gene="nrdE"
                     /locus_tag="ACG8H7_000701"
     CDS             8088..10247
                     /gene="nrdE"
                     /locus_tag="ACG8H7_000701"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP8982818.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10297..11262
                     /gene="nrdF"
                     /locus_tag="ACG8H7_000702"
     CDS             10297..11262
                     /gene="nrdF"
                     /locus_tag="ACG8H7_000702"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP8982819.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11351..12490)
                     /locus_tag="ACG8H7_000703"
     CDS             complement(11351..12490)
                     /locus_tag="ACG8H7_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8982820.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12799..13512)
                     /locus_tag="ACG8H7_000704"
     CDS             complement(12799..13512)
                     /locus_tag="ACG8H7_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP8982821.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13766..14467)
                     /gene="nagB"
                     /locus_tag="ACG8H7_000705"
     CDS             complement(13766..14467)
                     /gene="nagB"
                     /locus_tag="ACG8H7_000705"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP8982822.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14701..16233
                     /locus_tag="ACG8H7_000706"
     CDS             14701..16233
                     /locus_tag="ACG8H7_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP8982823.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16424..17626)
                     /locus_tag="ACG8H7_000707"
     CDS             complement(16424..17626)
                     /locus_tag="ACG8H7_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8982824.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17708..18397)
                     /locus_tag="ACG8H7_000708"
     CDS             complement(17708..18397)
                     /locus_tag="ACG8H7_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP8982825.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18503..18871)
                     /gene="rplL"
                     /locus_tag="ACG8H7_000709"
     CDS             complement(18503..18871)
                     /gene="rplL"
                     /locus_tag="ACG8H7_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP8982826.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18924..19427)
                     /gene="rplJ"
                     /locus_tag="ACG8H7_000710"
     CDS             complement(18924..19427)
                     /gene="rplJ"
                     /locus_tag="ACG8H7_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP8982827.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19631..19758)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19837..20526)
                     /gene="rplA"
                     /locus_tag="ACG8H7_000711"
     CDS             complement(19837..20526)
                     /gene="rplA"
                     /locus_tag="ACG8H7_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP8982828.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20630..21052)
                     /gene="rplK"
                     /locus_tag="ACG8H7_000712"
     CDS             complement(20630..21052)
                     /gene="rplK"
                     /locus_tag="ACG8H7_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP8982829.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21395..22063
                     /gene="sdaAB"
                     /locus_tag="ACG8H7_000713"
     CDS             21395..22063
                     /gene="sdaAB"
                     /locus_tag="ACG8H7_000713"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFP8982830.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22076..22948
                     /gene="sdaAA"
                     /locus_tag="ACG8H7_000714"
     CDS             22076..22948
                     /gene="sdaAA"
                     /locus_tag="ACG8H7_000714"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFP8982831.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23039..24139)
                     /locus_tag="ACG8H7_000715"
                     /pseudo
     CDS             complement(23039..24139)
                     /locus_tag="ACG8H7_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24231..25118)
                     /locus_tag="ACG8H7_000716"
     CDS             complement(24231..25118)
                     /locus_tag="ACG8H7_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8982832.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25195..25740)
                     /gene="nusG"
                     /locus_tag="ACG8H7_000717"
     CDS             complement(25195..25740)
                     /gene="nusG"
                     /locus_tag="ACG8H7_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP8982833.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25841..26011)
                     /gene="secE"
                     /locus_tag="ACG8H7_000718"
     CDS             complement(25841..26011)
                     /gene="secE"
                     /locus_tag="ACG8H7_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP8982834.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26035..26187)
                     /gene="rpmG"
                     /locus_tag="ACG8H7_000719"
     CDS             complement(26035..26187)
                     /gene="rpmG"
                     /locus_tag="ACG8H7_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP8982835.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26349..26993
                     /gene="cbpA"
                     /locus_tag="ACG8H7_000720"
     CDS             26349..26993
                     /gene="cbpA"
                     /locus_tag="ACG8H7_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFP8982836.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27049..27552)
                     /locus_tag="ACG8H7_000721"
     CDS             complement(27049..27552)
                     /locus_tag="ACG8H7_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP8982837.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27565..27611)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28149..29114
                     /locus_tag="ACG8H7_000722"
     CDS             28149..29114
                     /locus_tag="ACG8H7_000722"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP8982838.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29213..30385)
                     /locus_tag="ACG8H7_000723"
     CDS             complement(29213..30385)
                     /locus_tag="ACG8H7_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982839.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30372..>30497)
                     /locus_tag="ACG8H7_000724"
     CDS             complement(30372..>30497)
                     /locus_tag="ACG8H7_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982840.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9303 a         6264 c         5401 g         9529 t
ORIGIN      
        1 taattcatat ttttgttttt ctaattttaa atataccttt tgtaaaacct caagaatatc
       61 agaatattta ggatctttac ttatagatat atcattatat aattcatgaa caatttgctt
      121 agcatttttt ttcattttag cacttccctg gaattgctcc acctaaaaaa ccacctatag
      181 acattcctgc aatacaagta gttgccttgg cccaatcgac cgtacattta tttttagtgc
      241 aatacactcc atttccataa tattttccac tatgagtggt acccccatag ataagttgtg
      301 tctctttaat agacaaaatt tttaaatgtt tcataatcta atcaccccta aattaatgtt
      361 tttagactat tattctaaca ttcattaaat ttcaaaaaac aattccttct tgaaacgtag
      421 caaaataatt cctgaataag aatttaatca cttttaagta tataaactat gtatattctt
      481 taatattttt tgagaatgca cttcgtacat aataaacttt aatcacatta gtctttcata
      541 cctacgatcg atctctttga ctcgcaccca taaattcata atagtttgtc ggcgtctttg
      601 gacgtttcga agatgagcta cgcattctct ttttttatag acaaaaaaga tagagacgaa
      661 catggagctt ccaaatttgg atatttgatc caattttgga agctccattt atttcgtgtc
      721 tgtcttttta ttttttccag ctttcgacta attcttggtt ctctttgatc cagtcttttg
      781 ctgcttgctg tggatctttt ccattgttga tttccaacat aactttttcc atatcttttt
      841 ctgaccaatg gaaattgtct aatactttgt aagcttctgg ctggtcttct ttcaatcctt
      901 ttcgaaccat cgtatggatc tgctcttcgc ttcccattcc gttttcagga tcttctaagt
      961 atttcagatc atatttggca aacatccaat gaggtgtcca tcctgtaact acaattggtt
     1021 cgtgtttttt gatcgcttga cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta
     1081 ctttccagtc tttcaagttg tcatattttt ggatcgtgtt ttcagcagcc gtaactacac
     1141 cagctcctgg ctcgattcct atgattttct tacctgcctg atcggttaac tcatcgatcg
     1201 aatttacatc catgtaggct ggcactgcta aaccaacttt tgctcctgtc aaattggcac
     1261 ctaggtcttc gacttgatct ttatattgtg catattgtga accatgagtt ttaggtagcc
     1321 aagctgaaac catggcatct gactcgccat ttgaaacaga tttccacatg attgaattat
     1381 ctaatggcgt catcgttaca tcatagccca tttgtttcaa tacttcaccg actacatttg
     1441 ttgaagcaac ttctgtatcc cattcaacat aagataagtt gatacgtttt gtttctttgg
     1501 cagatgaaaa actccctata ccaatcgcac ctaagatcac tactactaca gcaccaatga
     1561 tcagtccttg acgtttttta cttttctgtg ttgttgctcc tgcagctttt ttattgtttg
     1621 gctggttcaa atgctgtgta aaacggtcaa tgatgattgc taagataact aatgcaaccc
     1681 cgttgacaaa tccattcccg acttgtgctc tttgcaatgc tgataatacg ccgcgtccta
     1741 agccgggtgc accgatcata gaggcaataa cgaccattga aagtgacagc attgttgttt
     1801 gattgatccc tgccatgatc gtacttttag ctaaaggcaa ttctagtttg aatagttttt
     1861 gacgacttgt gctcccgaat gaatcagaag cttctactaa ttctttagga acttgtcgaa
     1921 tccctaaatt cgtaaaacga acggttggtg gcagagcaaa gatgactgag gcaaataccc
     1981 ctggaaccat cccgataccg aaaaatgcaa ctgctggaat caaataaacg aatcctggca
     2041 ttgtctgcat aaagtcaagg atcggtttga taatactttg ggctttactg ctttttgcca
     2101 tcaagatccc taatggtacc ccaatgatga tcgagatcac actagataaa agaacaagtg
     2161 tgacagtatt catcaaatca ttccatagac cttggttata aataaaccat agaccgatta
     2221 atgtgaacaa cgtaagtcct ggacgtttct tagaaataaa gaaagctgct accgtcaata
     2281 acaagataaa caaaggagct ggaatcacta acagtaaatt tgtaatcccc gacatcactg
     2341 cctgtccaat cgtttgtaaa aatgaaaata ggccagaaaa agttgtcgta aaccattctg
     2401 tgatattttc tacccagttt gccactggta attgatagtt caatagatta ttcatgttca
     2461 ctcacctcgc ttgtttctgc taatgcttca atcacactac ctcggatgac gacacctttt
     2521 aattttccat cctctacaac tgcaagtgga gtcggtgagt cataaataat attgaaaata
     2581 tcacttacca ccatgtcttt cccaatcacc gtcacatttt catctaggaa gtcgatcaat
     2641 ggacgttttt gcttcctagc ttctaacgct tgatctgcag tgatgatccc tttcaggtgt
     2701 ctctttctgt caacagccat cagcatgctg acttcttcat tacgcatcct tgtcaatgca
     2761 acgtttggtc catcagattc gatattcgtc gtcaatgcag gtaccatgat gttttgagcc
     2821 gtaagcactt tggaacgatc gacttcttcc acaaattcac gtacataatc atttgctgga
     2881 ttggtcaata tttcttcacc tgttccgatc tgcatgattt cgccgtcttt catcaaggcg
     2941 attctgtccc caatccgtaa agcttcattc aaatcatggg taatgaaaat aatcgttttt
     3001 tgaacattgg cttgcaggtc cagtaattcg tcttgcattt ctcgccggat cagcggatca
     3061 agagccgaga acgcttcgtc catcagcaag atctcaggat catttgctaa agctctggct
     3121 aggccaaccc gttgttgcat cccaccagat aactgactag gatattggtc tttaaatgaa
     3181 agcaaactag aattttctaa tgctttttct gctttttctt gtcgttcttc tttaggtaca
     3241 ccacggactt ctaacccgta ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg
     3301 aagttttgga aaaccatatt gattttatga cgacggactt ctcgcaattc ttctttgctt
     3361 aacttcgata catcttctcc atcaatatag atactgcccg aagttggctc aatcagacga
     3421 ttcaatagac ggatcaatgt ggatttccca ctcccggaaa gccccatgat cacgaagatt
     3481 tctccttctt ttacgtcaaa attcacgtca tacacaccaa ccgttgcacc ggttttcttt
     3541 aaaatctccg ttttcggctg gtgatcattc atcatatcta aagcggcttg tgtcttttta
     3601 ccaaatatct ttgttaaatg ttttacttct acttttacca aacaaattct ccttttttat
     3661 gtaaattcat ttttctttct gaaaaaagtg accacactat gttaacatca tgtagtcact
     3721 ttgtcaaaaa tgaaagaaat ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt
     3781 aaataaaagg tttagccaag ttaatatttt tttaatattt cacacaatca ttctgcaagc
     3841 tgatgctccg ggtaaaaaaa gtttgtcata cgctgtagtg tatattcatt tccaataaaa
     3901 tgaacgatgt cccctgccgt aaatttagca taggggccag gtgaaacaag cagttcttca
     3961 ttatgagaaa ttgcgataac tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt
     4021 gaacggtcca gatgcattgc tttttctgat aaagataatt caaacggaag taaaggattc
     4081 atcttatgta ttcgcttcgt ttgattgacc agctgatcca ataattgaga aaaatgattc
     4141 agctcatctt gttgcttctc cacacttgcc atgatttctg cttttgtatc ttggatggat
     4201 tgtacatctt gatattgcaa taggaaatct tttgcttttt ccttcgaagc aacaaatgcc
     4261 ccacttccat gccgtacttc catgattcca agatctacca gcacattgat agccttgcgg
     4321 gtagtctcag gagaagcatt gtacgtactg gcaagtgttg agcgggcatg gatcttctca
     4381 cccactttat atcggttttc aacgatacgt tcggctaaat caacagcaat ctgctggtag
     4441 cgcgggcgga cgattttttt ctttccagac attttctttc ctctttcttt ttttactttc
     4501 cctcatctta ctatgctcta gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg
     4561 actgatcatc gcttgatctg ttcctttttt actgggcaag ctgtttgaca gtcatcacaa
     4621 ttcttaccgg attttaaacg agtatagaca attgcgcctg cacttccaaa gattaacaca
     4681 cttaataaga atgtcgccat cctattcctc tccttctaat gacaatatag tataaaactg
     4741 gttcttggat tttggctgac ggaacaaaac ataaacgagt cctatcatca gtaaaatagc
     4801 aagatacgtc cctaacgcta cctgtccacc ctccaataag tgtcccaact gataaatcac
     4861 aaaactaact aaataagcta aaccgcattg atagcctatg gcgatccaag tccatttctt
     4921 ggcattcatt tcccgatgga tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt
     4981 gaaggctaac aatgaataag ctgctgcggg agtatatgtg tgttgtagca cttgccagat
     5041 ttctctgcca ttttcggata cttccgcatg accaaacaaa ataccaaatg tgccaataat
     5101 attttcttta gcgaccaatc ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc
     5161 aagcggctga aaaacaactg ccaggattcg cccgaaaaag gctaagatac tttggtcttc
     5221 agaaacaggt tgagcataaa aattataaga agagctgaac cagatcacga tactagaaac
     5281 aaagataatc gttcctgctt ttttaacaaa agacttgctg cggtcatagg tttgtcttag
     5341 gaccgctgac cattgaggaa aatgatatgc gggcaattcc atgataaaag gagctgtatc
     5401 tccagcaaac aaacgtgttt ttttcaagct gattccagag aaaataattg cagtcatacc
     5461 aagaaaatag gcagaaggag ctacccaaga ttgatgagga aaaaaggccc ctgctactaa
     5521 ggcaataatc ggcaactttg cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg
     5581 acgatcttgc tcgttttcaa tcgtgcgact ggccatgacg cctggcactc cacatcctgt
     5641 agcaatcagc ataggaatga aggattttcc tgaaagaccg aatttcctga aaagtcgatc
     5701 catgacaaaa gcgatcctcg ccatatatcc gcagtcttcc agaaagctca agcataaaaa
     5761 caatataatc aattgcggta gaaatcctaa cactgctccc acgcctgcga tgatgccgtt
     5821 caagatcagc tcctgcatcc atcccgctac ttgccattca gctaacaatc tgccaacatg
     5881 ttctggtaac catgtaccaa acaatgtgtc attgatccaa tctgtcccca ttgttcctac
     5941 tgtttgaata gccagataat agatcagcca catcacaaat gcgaagatcg gcaaggctaa
     6001 ccaacgattc gttgcaatct gatcgatttt gtcactcatc gataattgga aagaatcatt
     6061 tgacacaaca cagagtgtac atagttgggt aataaattca taacgttcat tgaccagaat
     6121 cgtctcacta tcgtcgtcca atagtttctc tgttaactgg atcagttgct cgatctcttt
     6181 ttgctgagaa actgctatct cgtaatcctc tcttaccttc tgatctcgtt caaaatattt
     6241 cattgcatac cagcgtttct tgccgctttg ttctcctagt acatcttcaa tttcatgtaa
     6301 agctgcttct aaacgtggat catatgtaca gggaacagga atagaaggcc gcttttttgc
     6361 agactcctga atcttttcga ttagtttttc gattccttgc tttttcaata cgctgattgg
     6421 taaaactggt acaccaagac cataggaaag cttttctaga ttgatttttt ttccttcttt
     6481 tttgatgaca tccatcatat tcagaccaat gatcatagga atgtctaaat ccaataactg
     6541 cgtagttaaa taaagatttc gttctaaatt ggtagcatca ataatgttga tgatcagttc
     6601 aggctgttcc tgaaccaaat aatctcttgc aatcacttct tctggtgtat aaggagacaa
     6661 agaatagatg ccgggtaggt cctgaatatg aatgttcgga ttttttttca acgatccttc
     6721 ttttttttct actgttacgc ccggccaatt ccctacatat tgattcgaac cagtcaagag
     6781 attgaacaag ctagttttcc cgctgtttgg atttccgact aacgcgatat tcattctttt
     6841 tcctcccttt gtatcaagat tagttcggcc tctgactttc gtaacgtcag ttcataccct
     6901 cgcactgtca gttcgattgg atctcccaga ggggctagtt ttcggataac tatttttaca
     6961 tttcgcgtca atcccatatc cattaaccgt cgcttcactg cgccttgtcc gtggattgct
     7021 acgacgatcc cagtctctcc tgcttttaat tgatcaagag aagtccagct ttcagaggaa
     7081 gcagtctttt tattgacaaa gatttgacct agtatttccc gacttactgc cactcgatga
     7141 ctttttacca gtaaaatggc attgttgcct gataaattga cgacgacgat tttacttccg
     7201 gcaacgatcc cgatattttg caaatggcgt tttgtttttt cttctgtttc gattcgttcg
     7261 acttgataaa cgaaattctg ctttgcctgc gctaaggtct gcatattgat tcctctcttg
     7321 tgaaataaat catgtattaa aatcattcat tatatgttct aattttctta cgtgttacgc
     7381 aaataaatga gaatcattct cacatagaat actctcggta attgagaatg tcaattatac
     7441 ttgtgatatt atgagcgaat ctttagtatt gatagataaa aatgagaaaa aaatatttct
     7501 tcatagtttt ccacaatttt ttctctatta aatttgctat tttcccacac gatacttgtt
     7561 ttcagctctt ttttcaaaaa cacaaaactt agtattccac agtttccaca acacaatatg
     7621 tagtgtttgt tgtttgactt ttttactaag agttttatac tatctattat agataataag
     7681 aatgtgttag aggaggcact caccatgaac gtaaaaatct tttctaaaaa taattgtatg
     7741 caatgtaaga tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat
     7801 atcgatgaac agcctgaagc agtagactgg ttgaaagcac aaggattcca aagtgttcct
     7861 gtcattactt ctgatgcaga aacagttatc ggattccgtc ctgatcagtt gcgtaaactt
     7921 gccagctaac atgactaaaa gaatcaaaac gtgatacatg gatttgaagg gcttgagaca
     7981 gcaccagttt ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac aatgcaatga
     8041 tttgcaatca agcatcatca aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa
     8101 atttacaaga tgtcagttat ttcaagctga ataatgaaat caatcggcca gtcaatggtc
     8161 aaatcccttt gaataaggat aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac
     8221 caaatacgat gacttttcta tctattatgg aaaaaatcaa ttacttgatc gatcaagaat
     8281 atatagaagc agaattcatt caattgtatt caccatcatt tattgaaaag ctatatcaat
     8341 ttttggacga gcaacatttt tctttcaaat cttttatggc tgcttacaaa ttttattctc
     8401 aatatgcgtt gaaaaccaac gacggttccg cttatctgga aacttatgaa gatcgagtag
     8461 ccttcaatgc gctttacttt gcgaacgggg atgaagaatt agcacttgct cttgcagacg
     8521 aaatgatcca tcaacgatat cagccagcta caccttcttt cttgaatgct ggacgtaaac
     8581 gtcggggcga attggtgtct tgtttcttga ctcaagtcac tgacgatatg aactctatcg
     8641 ggcgctcaat caactctgct ttgcaattgt cccgtattgg tggcggcgta gggattactt
     8701 tatcaaatct tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct gcaagtggtg
     8761 tcgttccagt catgaaatta ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc
     8821 gtcaaggagc tggcgtggtt tatttgaacg ttttccatcc agacattatt gctttccttt
     8881 cagcaaaaaa agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga
     8941 tcgtacctga taaattttat gaattgacac gtaaaaacga agatatgtat ttatttagtc
     9001 catatagtgt cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt acagctgagt
     9061 atgatcatct agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg
     9121 aaaatgaaat ctcaaaatta caacaagaat ctggctatcc ttacgtaatc aatattgata
     9181 cagcgaaccg cgagaatcca atcgatggca agatcatcat gagtaattta tgttcagaga
     9241 ttttacaagt tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa gtattaggaa
     9301 cggatatctc atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa agccctaact
     9361 ttggtcaatc tgtgcgtacg atgactcgtg cattgacatt tattacagat gcatctgata
     9421 tcgatgtcgt accaccgatc caaaacggaa ataagctgaa tcatacgatt ggattaggtg
     9481 ccatgggctt acacacttat cttgcaaaag aacaaatgga atatggctca gaggattcat
     9541 tagactttac aaatatttac ttcatgctgt tgaattattg gacacttgtt gaaagcaaca
     9601 atatcgctcg tgaaagaaaa caagttttcc ataattttga aaaatcagct tatgctgacg
     9661 gcacttattt cgaaaaatac gtaactggag aattccagcc aaaatcagaa aaagtcaaag
     9721 cattgtttga aggcattttt atcccaactg ctgaagattg gaatgctttg aaacaagcag
     9781 taatgaaaga tggactgtat catcaaaatc gcttggcagt agcaccaaat ggctcaattt
     9841 catatatcaa cgatacgagt gcaagtattc atcctatcac tcgcatgatc gaagaacgcc
     9901 aagagaagaa aatcggtaaa atttattacc cagcgcctta tctatccaat gagacgattc
     9961 cgtattacac ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt tatgcaatgg
    10021 cacaacaaca cgtcgatcaa ggcatgagtt tgactttatt catgcgttct gaaatcccag
    10081 aaggcattta tgaatggaag acaacaacga agcaaacgac acgtgatttg aatattttaa
    10141 gacattacgc tttccacaaa ggaatcaaat ccatttatta cgtacgtacc tttactgatg
    10201 atgcagaaga aatcggaagc aatcaatgtg aaagctgcgt gatctaatat catagataga
    10261 ttcgcttatt catattttaa ttaaaggagc attctcatgt cagaaacata ttatgaagca
    10321 ataaactgga atgaaattga agatatcatt gataaatcca cttgggagaa gctgacagag
    10381 caattttggc tagatacacg tatcccactt tcaaatgacc tagatgattg gcgtacattg
    10441 tctcaattgg aaaaagatac agtaggacat gtatttggcg gtttgacact gctagatact
    10501 gtccaatctg aaagtggcat ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa
    10561 gccgttttga acaatattca attcatggaa tccgttcatg ccaaaagtta ttcttctatt
    10621 tttagtacac tgaatacgaa aaaagaaatc gaagaaattt ttgactggac aaatacgaat
    10681 ccttatctgc aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg tacaccgcta
    10741 gaaaagaaaa ttgccagcgt ctttttagaa acattcttgt tctattcagg tttttatact
    10801 cctctttatt atttaggaaa caacaaattg gctaacgtag cagagatcat caaactaatc
    10861 atccgagatg aatctgtaca tggaacctac atcggttaca aattccaatt aggattcaac
    10921 gagttacctg aagcagaaca agaaacgtta aaagactgga tgtacaatct tctttatgag
    10981 ctttacgaaa acgaagaacg ttatacagaa gaattatacg atccaatcgg ctggacagaa
    11041 gaagtcaaga cattcctacg ctataatgcc aacaaagcat tgatgaacct agggatggat
    11101 ccactctttc cagatactgc caatgatgtc aacccaatcg taatgaacgg gatttcaaca
    11161 ggcacaagca accatgactt cttctcacaa gtcggaaacg gctatttgtt aggtcatgta
    11221 gaagcgatga aagacgacga ttatttgatt ggattggaat aaggatttat tttaattcaa
    11281 aaatttttcg aaaaataaaa agagtttagc gtacagatat tttaaatgta cactgaactc
    11341 ttttttatgc ttattcactt gtttcttcca gcacttttgg actgtctaac ttttccgaaa
    11401 catgttccat tcttctcaat aaatagattt tccggatatt taatattatc gtcttaatca
    11461 ctgcatagac aggtatgcaa atcagcatgc ctaatattcc tgcgacactt cctgctccaa
    11521 tcaataccaa gatgatcgtc aaaggatgga tattcattga tttcccaaat aacaacggct
    11581 tgatcaaatt tccatctaat tgttggatca ctaaaatcga caacgccata tacaaagctt
    11641 gaaatggaga gataaacaaa ccgacaatga ttgctggtac cgctccaata aaagggccga
    11701 catacgggat aatattcgtg attccgcaaa tgaccgcaag caacaagcta taaggctgat
    11761 cgaatattct cattgccaaa aagctcatta caccaataat caaagcatct aagatcgtac
    11821 tgctgatata ggcagataat gtttcgttta attcttttac cgtttggcgt aactctttac
    11881 gaatcgcttt cggaaagaag tgagacaacg catcaataaa tttgtgccca tccttgaaca
    11941 taaatagcaa aataaatgga acggtaaata acaaaataaa aaattgcatc ataaatgaaa
    12001 tgatttttga gacacttgca gataaactga ctaatatgat attgaacaaa ttactaagag
    12061 ataaatttgc tgatgctaat tgttcttcca aattgaaatt tttaaactct tcccgctgcg
    12121 ccaattcatt taaccatttg cttgtttcat ctgcatacat tggcaagctc tgggtcaatt
    12181 gaattgtctg ttccattaac tgaggcacta cattcatgac agttaaaaca actaaaatga
    12241 ttacgaccaa aaacgtcaat aaaaaaccga acactctcgg gatccgatgc ttttcgagaa
    12301 aaacaacgat tggatcgaac atataataga taaaaccagc aacaagcaat ggtaaaaaaa
    12361 ctgccgaaag cattttcaat attggactaa aaatattcgg catttgtaaa ataaagtaca
    12421 cgccgatgat cgtgagtact agctccactg tccagaaaaa taattttgat tgtttaaaac
    12481 gtgattgcat acatctcctc caaggtcctt tgatacttct attctatcat ttaacaaaga
    12541 gttacgcgct gagattttca tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg
    12601 agtagacgag tacttaaaca aaaacacggt ttggagtagt gacaaaaatt atactgcagt
    12661 ccctttttcc aaataaacgg tgttcaaaag cttgcatctt cgtaataagc ccagccaaac
    12721 gaaaaatata aggaactatt tttatttggc tgggcttatt acttgatgta ggacatctat
    12781 tttacaacct cagatcattt attgcgcaat actgactaaa tgcaactttg tgatattttt
    12841 gtgtacggac aactcctcga tcacatcggc ataagtgagc ccttttggaa gatcaatcgt
    12901 atagatattt ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc
    12961 gattacgata tttttctctt caaaataatc ggtaagaaaa tctttggttt cttttctatg
    13021 gataaattgg atttctaact ttttagtcgt aggtacatgg atcacgcgtt tcactactgt
    13081 gagtgcaata gcaatcccga taaaactgat gatcgcaatg gaatagtaac ccatgccgat
    13141 ggcgatccct aatccagcaa ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga
    13201 ttgtttcgtg acgatgatcg ttcccgcgcc taagaaaccg acaccactca ccacttgagc
    13261 aatcagcctt gcttcatctg aacgaatcgt tccagcaagc tgtggatact ctaaagcatc
    13321 tcttaatgca ccactagcga tctcaacttg gatcaatgca atgatacatg ctcccataca
    13381 aactaaaata tgcgtacgca tacctgctgg acgattttta tattgtcgtt caaaaccgat
    13441 tgctccgcca aatagcatag cgatccctaa acgtaaaata atctctggaa ttgtcaatac
    13501 catatcattc atctaaacac cctccagtcc aatatatcat gtctgaaagg agcagacaaa
    13561 aaaagtgcaa tttttacctc ttacgaaaaa aggactgtga caaaattttt gtcatagtcc
    13621 tttttatgaa taaacggtgt tcaatcagct gaccctcggt attactcaac aaacggcaaa
    13681 atatggggag ctatttttcc tttgtcttct ctaatactca ggtcaaaacg ctgatttcac
    13741 aacctcttta gcctactatt gaaaattaca acttacttgc tgcttcttca tcgatgatca
    13801 caacaacatt gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt acaggtcctt
    13861 caatcatccc tttgattgca tcagctttgc tttcgccaaa tgccatcaag acgatttctt
    13921 tacttttcat aattgaaccg attcccattg aaacagcttt tgttggtaca tcttctgctt
    13981 tatcgaagaa tcgtttgttt gcattgattg ttgattctgt taagtcaact actgaagtcg
    14041 ttccgtcaaa tgaagcacca ggttcattga aaccgatatg tccattttgc ccgattccta
    14101 agatttggat atcgattgga tgtgcatcga tgatttgatc atactttttg catgctgctt
    14161 ccagatcttc tgcttttcca tctggtacaa acgtttcttt gaatggtttt ttatcaaata
    14221 attggacatc catgaaatga cggtagcttt gttcatctga accgcctagt ccaacgtact
    14281 catctaagtt tacagaagtc atttcactga aatccaaatc gcttgaagtc atttctttat
    14341 ataaagtaac tggtgtgctt cctgttgcca atcctaatac ttttgcacct ttgttcatac
    14401 cttctttgat gatttcaaat gctttttttc cgccttcttc agcgtttttt acacgaatga
    14461 tttccatagt gattgctcct ttatattttg gtatagccca attatagcat tctttttttc
    14521 tttttacaat aattagtata gaccaaattg catttttttc attttcttga caataaatag
    14581 gaatccgtgt taggtttcat aagatggttc aatcaaacaa tccatgttgc atgaaaacca
    14641 agcaagtgat tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa aggagttatt
    14701 atgagtatac tgacattaaa caacattacc cagcaatttg gcgacaaaat cctctatgaa
    14761 ggtgtcgatc tgcaattaaa tgctggcgaa catcttgggc tgattggaca aaacggagcc
    14821 ggtaaaagta cattgattaa aatcatcact ggtgagatct taccagatga cggacagatc
    14881 cattggcaaa aaaatattca caaaggttac ctcgatcaat atgtggaagt cgatgaaaca
    14941 ttaacaatcg aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa
    15001 attgcttttc tttaccaaac atatagcgag accttcgatg attccttgct ggaaaaagct
    15061 gggaaactcc aaacggaatt agaccaagga gtgttttatc aaatcgacac cttagttgct
    15121 gagatgagca gcgggttggg gatcgatgtc ttaggtttag attcactgtt gaaaaatcta
    15181 agtggtggac aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa cccagatgtt
    15241 ctgatcttag atgaaccaac caatcactta gatgatcagc atatccaatg gctgacgcag
    15301 tttcttcagg attttgacgg tgcgtatatc gtgatttccc atgaccaaga atttcttgat
    15361 cagatcacca cccatatcgc agacatcgag tttggaaaga ttactaagta tactggccat
    15421 ctaaaacaag cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat
    15481 gctcaacaaa aacaaatcga aaagaccgaa gcttatattc gaaaatacaa agccggttcc
    15541 cgatcaacga tggcaaaaag ccgacaaaaa caattagata aaatcgaacg gctgacgcct
    15601 ccagcttcag caagtaaagc tgtctttgat tttccattct cgcctattgt cacgacatta
    15661 gcagtggaaa caactgattt agtcatcgga tatgatcgac cgctacttgc acccatcaat
    15721 ttgaccatcc gctttggtga aaatattgcc attcgaggct tcaacgggat tggaaaatct
    15781 actttattaa aaacactgat cggtgagatt gaaaaaataa gtggcgattt ccacttccca
    15841 gataacacaa aaatcaatta cttttcacaa gatctgtact gggaaaatcc attagaaaca
    15901 ccactacaat atttaagcag tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa
    15961 ctagctaaag cagggttagt taatcaatta gcaagtgaac ctctcactac attaagtggt
    16021 ggggaacaaa ctaaggtgaa acttgcacag ttgacaatga accaaggtaa tttactgatt
    16081 ctcgatgaac cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca agacgggata
    16141 cagaaatttc caggaacagt aatcatcgtc tctcatgaac aagaatttta tcaagatctt
    16201 gtgcaacgag tcattgagat tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag
    16261 gagctgtgac aaaagttttg tcacaactcc ttcttctgaa taaatggtgg ccaattagct
    16321 gaccttcggt attagctcaa aaaacggcaa aatatgagga gctattttta tttggctatt
    16381 cttattactt gatgcagaac agcttttcac aacctctttt cgtctatttt ttattttcga
    16441 gatttctctt tcctttaaaa acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa
    16501 gcccgctaac cgttacacct aaccagccat actgctgcca cattgtcgtc cctagcaatg
    16561 aaccagccgc acctccaata aagtacataa acataaatac agtattattt cggttgcttg
    16621 ctgcttcacc tagcccttgt actcgtgttt gattggcgac ttgaccaaat tgattcccga
    16681 cgtctaataa aataatagct aaaagcaaaa tggccaaatg gcttccgcca accaataaag
    16741 caataaagct tattgtttgc ataacaagtc ccatatacac gatttttctt tcagaataag
    16801 aatctgccag tcgtcccacg ataggtgcag caactgctcc agacaaacca aaaatcgcta
    16861 atatccctgc ttcgaatgtt ccccaattgt aggcgggact gctgatataa aaaatcaacg
    16921 tagaccaaaa gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc gcttctctta
    16981 atagacgttg agaagcaact aaatgcggca aacttttaag tgaatcaagg tatgaaacag
    17041 ctgtactact ttgctggaca gaacgcggca ttttccagta aagcagaagt gtaagaattc
    17101 cgactgcaca taaagcaaat aaatagatca tccgccaaga aaaaagactg gcaacaatac
    17161 cagataccgt ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat gtacccatga
    17221 ccttccctct tgcttttggt cccgctagta ccgcaccgta aggaatgatg atttgcggca
    17281 cgatcgaaag taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac gaaggcgcaa
    17341 aaaaagcggc caacagagag acggcagata acgctgccat tcgaatgatc aattttggac
    17401 gatccaccac gtcccctaaa ggaactaaaa acagtaaccc taacgcatag cctaattggg
    17461 tcaacatcgt caatatcccg actgcacttg tgctaacaga taaactagct gctacttttg
    17521 ttccaatcgg ctggatgtaa tacatattgg caacaacaac gccacaagtg attgataata
    17581 gtaaaataag cgaagttgtt aaaggttttt tttcattact cttcattgat ttcccccatt
    17641 tcctatctta gtttacaact tatggaaaag attggaaata gaaaaataca actattcgcc
    17701 acacttatta tttgtgcttt tgttttcctt gttttttctt gcgatcttta taaatgaaga
    17761 caactaaccc tactagcaca gtaacacttg caaccaacgt gactgcagtt ttgttatatc
    17821 cgtgaaacag agcttctcca ccacacgcat acaacacaga cgaagggatg actccaatca
    17881 cgatcgacaa tatgatttgc tgtagaggca atttcatcgc atctgcagca aaattgacca
    17941 tagaagatgg aataaccggt atcatatagc ctaacattac acctattttt ggatgtttca
    18001 tttgtcggat tgcttgcacc caatgattcg tttcggtttt cttatctaaa aattttaagc
    18061 gatgcataag gaaaattgca gtgatgttac caagagcatt acccaaaatg ttgatgatcg
    18121 cacctgtcag tggtccaaag ctcaaaccag caatgactcc aactactgaa gtaggaatac
    18181 ctggaacagc acacatcacc gctgtcaata cgatcaagag tatcgatgca aaagtcccat
    18241 gcgaacgaat atgtgcgagt agctctgcct tactcgctct cggatttaaa aataattgga
    18301 tgtctgtctg atattctaaa tacaaccgat agcccactac cagtatcaga atgattccca
    18361 acgagatcaa tatcaatcgg attattttct ttttcatatt tcctcctgaa aaaaatctat
    18421 attcattaaa taatagttta acataaaaac aaaaaagcac cgaaatccaa cataggatct
    18481 cagtgctttt aaatagtaaa aattatttaa cagttacaga tgcgccaact tcttcaagtt
    18541 tagctttcaa ttcttcagct tcttctttag atacgccttc tttaactggt gcaggagcgc
    18601 catcaactac agctttagct tctttcaagc ctaaaccagt tgcttcacga actgctttga
    18661 taactttaac tttttggtct ccagctgcag ttaattctac agtgaattct gtttgttctt
    18721 cagcagcagc agcgccacca gcagcagcta cagctacagg agcagcagca gatacgccaa
    18781 attcttcttc aatagcttta actaggtcgt taagttctag gatagttgct tcttttaact
    18841 cagcaacgat gttttcaatg ttcaatgcca ttataatttc ctccgtttaa ttgttctttt
    18901 ttatttaatg ctttatgcgg caattaagct gcatctccat cttctttgtc tgccactgct
    18961 ttgacagcgt aagccacgtt gcggactggc gcttgtagta cagatagcaa catagataga
    19021 agtccttcgc gatttggcaa ttttgccaat gctgtgattt cttccaatga agagacttta
    19081 ccttcgatga taccgccttt gatttctaat gcttttgctt ctttagcaaa ttcgtccatg
    19141 attttcgctg gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg tccagtgaat
    19201 acttcgtcca tgccttcaag gccagctttt ttagctgcac gtgaaagaat agaatttttg
    19261 ataactttca tttctacgtt agcatcacga agttgtttac gtaaacgtgt tacttcatca
    19321 actgttaatc cgcggtagtc aacgactact actgatgcag ctgctgtaaa tttttcgctt
    19381 acttcatcaa cgattgctgc tttttttgcg ataattgctt cactcatttt tgagtttcac
    19441 ctcctgaatt ttgatggaag agttttttgc actgaaaagg caagtcactt ttatagttac
    19501 tttgctcaac cagtattatt catcacctat tcacttcgtt catagtgatt ctactcgctt
    19561 tgctcgaaag gaaactcctt tcgctttgct caaccagtat tgttcatcat ctcttcactt
    19621 cgttcatagt gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga
    19681 atatattcaa taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg
    19741 tacaagttaa ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta
    19801 tttttcagtt ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg
    19861 gataccaggt ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc
    19921 tgatggttta gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc
    19981 atcgtcaaat gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta
    20041 agttacttta ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc
    20101 agttttaggg tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt
    20161 agccatcata tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt
    20221 ttgaaccatg tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt
    20281 gtctccttta gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac
    20341 agcaccacgg atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc
    20401 aactgttgca tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac
    20461 aggataaact ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt
    20521 agccattttg gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata
    20581 tctttcaata tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag
    20641 tgatccccat gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg
    20701 cgtttaggtc ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg
    20761 caactttatt tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata
    20821 gaacagcagc tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa
    20881 taacaaccgg aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga
    20941 atcccatgat attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg
    21001 ctttacctgc aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata
    21061 cctccttaag tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc
    21121 tgatctgaaa aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca
    21181 aatttatttg ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc
    21241 taaatttatt ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt
    21301 gcgctagcat ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac
    21361 aaagattcct acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt
    21421 ctttgatatt attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc
    21481 tagaatcggg aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta
    21541 tctatatgaa tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg
    21601 cggcctgtta gggatggaac ccgatgatga acgtttagct gattctttga aattggcata
    21661 tgaagccggt atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc
    21721 tgtaaagatg cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg
    21781 cggtggcaat atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac
    21841 tcctaccttg atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat
    21901 actttctgag accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga
    21961 aaatgccatt atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact
    22021 caaacagctg ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt
    22081 tttatcgatt gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat
    22141 gattgcagtt gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga
    22201 aaaaaacctc tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt
    22261 cacaggaatt accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt
    22321 tttaagtgga gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa
    22381 tgcaaaaatg gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg
    22441 tgtgctgatg gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt
    22501 atttaccgct ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga
    22561 agggggatgt caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg
    22621 tgcaaaaaat gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat
    22681 gatgggactg atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa
    22741 tgctttaggc tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag
    22801 tgtcattccg ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc
    22861 gattttcaaa gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc
    22921 ggaagagatt ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt
    22981 agttatcttc ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt
    23041 aatccatcaa tttcgtattt tttacttcat cgatcgtttt tgttcctgca agttgcatcg
    23101 tgatcgacaa ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta
    23161 gattcaaccc ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag
    23221 ctttgaagac atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt
    23281 tgactacttc ggcaattttt ggcaatactt caaaggacgc aggacctcca tctagttgac
    23341 gtccaccatg attggatacc cagataccat ctgcccctgc agagatagcg attactgcat
    23401 cttctggcga ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt
    23461 tgatgtcatc tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc
    23521 cttcgccatt accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg
    23581 gaaattggaa ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg
    23641 ttaaaatgat agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc
    23701 tgtcgtcttt actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg
    23761 cgtctttgat tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta
    23821 ctcctttagc tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg
    23881 cttggataat cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt
    23941 cgattccgcg taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt
    24001 tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc
    24061 gctcttttac acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat
    24121 tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat
    24181 actaaactta caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc
    24241 caaataaatc gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag
    24301 gcattttcac ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca
    24361 tagaccgcta atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt
    24421 tgaccagcaa tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa
    24481 tcttgatagc ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc
    24541 gcactatcat cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt
    24601 ggcaatgacg tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg
    24661 gaatgcccca ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa
    24721 gttttgatcc attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg
    24781 accatcggat tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt
    24841 cctactgtta aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc
    24901 cctttaaagg aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt
    24961 ttttctatca ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt
    25021 atcttcgtcg tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca
    25081 gttgcacgtt tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag
    25141 aaaaaagaaa gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc
    25201 gattttatct acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat
    25261 gttgactttc aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc
    25321 aaatgcgcct tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg
    25381 caggctcata ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg
    25441 tttgcttccg gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac
    25501 ataccatgaa gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg
    25561 aacgatttct ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac
    25621 acggaaaata aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac
    25681 tttgttttca tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat
    25741 tgaaggctcc tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt
    25801 cgcttcatta tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa
    25861 ataagctttg gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt
    25921 cgatgactac taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga
    25981 tttcatcttt tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt
    26041 cgtttcttta tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg
    26101 ttcgccgctt tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca
    26161 agcgagtgct gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta
    26221 tacttcataa cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt
    26281 gttttggata aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag
    26341 gtatagcgat gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag
    26401 agactagtac attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc
    26461 cgatcttaga ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc
    26521 gtcataaagc aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta
    26581 caaagttcat ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc
    26641 cttatattgc tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt
    26701 tgcttaatat gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg
    26761 cttccacagg aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca
    26821 gcattgccag ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga
    26881 tcacgctacc ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga
    26941 aaaagaatta taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa
    27001 gcgaaaaaag aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt
    27061 ttcaaaatca agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat
    27121 aaactcactt aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac
    27181 taacatataa gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg
    27241 catctttaca tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt
    27301 ttggccgcca ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat
    27361 gaccccgcct aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact
    27421 ttctgaaata cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc
    27481 tagataaaga gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt
    27541 cgttttttgc attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga
    27601 accgcggtta tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc
    27661 ttatctgctt ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg
    27721 atatggtata aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg
    27781 atctgcaaga acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga
    27841 gcaacctctt actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa
    27901 ccttggaact tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt
    27961 ttcggtcttc ggaccttttt cattttccta gacttctgct ataataacca agaattaatt
    28021 ttgtaacaga tttttttgaa aaaacaagtt tctttcattc aaaaaataga aaaagcttgc
    28081 taattattgg ttaaccagca aacaggagta ttaaaatatc ttgttttatt gaatagcagg
    28141 aggaaatcat gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg
    28201 gctataaagt agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac
    28261 ttcattatag tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa
    28321 cattgattcc ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca
    28381 tcgtaggagg tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag
    28441 aattcccagt tgcttatatt ccagcaggtt ctggagatga ttttgctcga ggggcaggcc
    28501 ttccaaaaaa tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag
    28561 tccacataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt
    28621 tcggtatagg attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc
    28681 gattgaacaa atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat
    28741 tcactcaaaa aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa
    28801 acgcattttt atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc
    28861 caactgctga tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct
    28921 ttaaaatctt gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt
    28981 tccatcacta cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag
    29041 aagatggaga agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt
    29101 tgttttggtg ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg
    29161 gaaaaaagga ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct
    29221 ttgcccaatt gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg
    29281 aaaacaccgc agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg
    29341 tttgattgta actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg
    29401 gtgtgaatgc cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc
    29461 caccgataaa gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt
    29521 tttttaccaa tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag
    29581 tatttaaaat atgataccat accgttatat cccacgaggt gtggatcact aacagaacca
    29641 ttagaagtaa gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt
    29701 tgcgattcaa gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc
    29761 gattaaatgc tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag
    29821 acaagaaatt aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac
    29881 ttagctcaac ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga
    29941 ttgttttatc ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg
    30001 taacaactgt tttagaaaag gcatctgggc gactctgtat cttgatcgtc ggcatctttc
    30061 ctacttcgac ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa
    30121 aataaagagc atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct
    30181 tgtcattcaa atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg
    30241 cgattttcgc atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg
    30301 tggcaacaac agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg
    30361 tccaaatcgt tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac
    30421 gcaaaaagga aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca
    30481 gtgtagaaat aaagttt
//
LOCUS       DAZKAS010000019        30457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000019 DAZKAS010000000
VERSION     DAZKAS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.019"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG8H7_000725"
     CDS             288..695
                     /locus_tag="ACG8H7_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982841.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG8H7_000726"
     CDS             709..1110
                     /locus_tag="ACG8H7_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982842.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACG8H7_000727"
     CDS             1112..1483
                     /locus_tag="ACG8H7_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982843.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1821)
                     /locus_tag="ACG8H7_000728"
     CDS             complement(1519..1821)
                     /locus_tag="ACG8H7_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP8982844.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2079..2192)
                     /locus_tag="ACG8H7_000729"
                     /pseudo
     CDS             complement(2079..2192)
                     /locus_tag="ACG8H7_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2575..3807)
                     /locus_tag="ACG8H7_000730"
     CDS             complement(2575..3807)
                     /locus_tag="ACG8H7_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP8982845.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4061..4630
                     /locus_tag="ACG8H7_000731"
     CDS             4061..4630
                     /locus_tag="ACG8H7_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8982846.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4808..5248)
                     /locus_tag="ACG8H7_000732"
     CDS             complement(4808..5248)
                     /locus_tag="ACG8H7_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP8982847.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5406..6170
                     /gene="map"
                     /locus_tag="ACG8H7_000733"
     CDS             5406..6170
                     /gene="map"
                     /locus_tag="ACG8H7_000733"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP8982848.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6202..7125
                     /locus_tag="ACG8H7_000734"
     CDS             6202..7125
                     /locus_tag="ACG8H7_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFP8982849.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7201..8340
                     /locus_tag="ACG8H7_000735"
     CDS             7201..8340
                     /locus_tag="ACG8H7_000735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFP8982850.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8333..9133
                     /locus_tag="ACG8H7_000736"
     CDS             8333..9133
                     /locus_tag="ACG8H7_000736"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8982851.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9133..9960
                     /locus_tag="ACG8H7_000737"
     CDS             9133..9960
                     /locus_tag="ACG8H7_000737"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8982852.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9965..10672
                     /locus_tag="ACG8H7_000738"
     CDS             9965..10672
                     /locus_tag="ACG8H7_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8982853.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            10772..11638
                     /gene="rfbA"
                     /locus_tag="ACG8H7_000739"
     CDS             10772..11638
                     /gene="rfbA"
                     /locus_tag="ACG8H7_000739"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP8982854.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11652..12224
                     /gene="rfbC"
                     /locus_tag="ACG8H7_000740"
     CDS             11652..12224
                     /gene="rfbC"
                     /locus_tag="ACG8H7_000740"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP8982855.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12246..13274
                     /gene="rfbB"
                     /locus_tag="ACG8H7_000741"
     CDS             12246..13274
                     /gene="rfbB"
                     /locus_tag="ACG8H7_000741"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP8982856.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13372..14223
                     /gene="rfbD"
                     /locus_tag="ACG8H7_000742"
     CDS             13372..14223
                     /gene="rfbD"
                     /locus_tag="ACG8H7_000742"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP8982857.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14258..16291
                     /locus_tag="ACG8H7_000743"
     CDS             14258..16291
                     /locus_tag="ACG8H7_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982858.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16335..17615
                     /locus_tag="ACG8H7_000744"
     CDS             16335..17615
                     /locus_tag="ACG8H7_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFP8982859.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17825..18631
                     /locus_tag="ACG8H7_000745"
     CDS             17825..18631
                     /locus_tag="ACG8H7_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8982860.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18643..19863
                     /locus_tag="ACG8H7_000746"
     CDS             18643..19863
                     /locus_tag="ACG8H7_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982861.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSNGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19853..21439
                     /locus_tag="ACG8H7_000747"
     CDS             19853..21439
                     /locus_tag="ACG8H7_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8982862.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21478..23616
                     /locus_tag="ACG8H7_000748"
     CDS             21478..23616
                     /locus_tag="ACG8H7_000748"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8982863.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(23984..24967)
                     /locus_tag="ACG8H7_000749"
     CDS             complement(23984..24967)
                     /locus_tag="ACG8H7_000749"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP8982864.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            25345..26736
                     /locus_tag="ACG8H7_000750"
     CDS             25345..26736
                     /locus_tag="ACG8H7_000750"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP8982865.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            26752..27792
                     /locus_tag="ACG8H7_000751"
     CDS             26752..27792
                     /locus_tag="ACG8H7_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8982866.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            27811..28896
                     /locus_tag="ACG8H7_000752"
     CDS             27811..28896
                     /locus_tag="ACG8H7_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP8982867.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            28929..29891
                     /locus_tag="ACG8H7_000753"
     CDS             28929..29891
                     /locus_tag="ACG8H7_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFP8982868.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            29884..>30457
                     /locus_tag="ACG8H7_000754"
     CDS             29884..>30457
                     /locus_tag="ACG8H7_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HFP8982869.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSII"
BASE COUNT         9932 a         4936 c         6044 g         9545 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
     1801 tgtttttcct tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct
     1861 ttttgtgaag gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta
     1921 tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag
     1981 atttcctgca ataacaaaca caacagaaga cgtgtaatag ttagtcagga acgtacaaat
     2041 aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct
     2101 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
     2161 atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa
     2221 ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag
     2281 attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt
     2341 tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga
     2401 tgacttttta ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa
     2461 agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct
     2521 tagatccttt attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc
     2581 ccaatcgcca tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc
     2641 catcttgtca gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc
     2701 tgctaattct tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc
     2761 tagtgctaag tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga
     2821 ttgagaaata ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc
     2881 atctgtatca agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaacaac
     2941 ttttccatct ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg
     3001 ctttgtactt gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc
     3061 cgtcggcata tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca
     3121 aagatggttt ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgaagggc
     3181 agaaaattgt tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt
     3241 ccatgctaag acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa
     3301 tgcctcaact tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc
     3361 agccgcagcc acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat
     3421 tagtgctttg ccattcgctg attgataaga cgctactcga tggctatcta caccagcaaa
     3481 tgcatcggga tcggcagaaa caacgctgat cctgcttgct cctttttcaa gccattcatc
     3541 cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag
     3601 aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt
     3661 ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac
     3721 aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt
     3781 tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta
     3841 actattagca tgataccatt taattatgat tcaaacaatg aggctttaaa taaaaaaagg
     3901 aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt
     3961 gaagaaaatc aaaaactaat tttgcatatc aaaaaaacat gttacaatga aattaatcaa
     4021 tggtggaagc gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat
     4081 tactagagaa cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg
     4141 atttactgcg agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga
     4201 gtttaaaaat atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca
     4261 atatgaagtg ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta
     4321 tatccatttt gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg
     4381 tggaggaaaa gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga
     4441 tccagcttat gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat
     4501 catagctact gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca
     4561 gattgaaaag atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga
     4621 aatcgattga atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa
     4681 taaggaaaag ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca
     4741 tgaatgcaat gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc
     4801 aagtatatta aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt
     4861 cttcttcagc attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg
     4921 caaaagcagc atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag
     4981 atccgcagac accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt
     5041 ctacgatctc atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat
     5101 ctgcctcctc gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat
     5161 ccagattttc aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc
     5221 tagcatatac gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa
     5281 ttatgaatta taccaaaaca tcattgaaaa gaaaataaaa cctaaagaca cctggggaat
     5341 gtcatttggc taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg
     5401 aaaatatgat tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt
     5461 tattagcaga tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg
     5521 atattgaggt atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg
     5581 gctttgaagg gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat
     5641 tccctcgtaa aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt
     5701 tgaaaggcgg catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga
     5761 tcgaccgatt gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag
     5821 tagggaatcg gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc
     5881 tagcgatcgt tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag
     5941 cagttcctca ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca
     6001 cgatcgaacc tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga
     6061 ctgcgtatac aagagacggc ggactttctt gccaatatga acatacattg gctattacta
     6121 aagatggtcc aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt
     6181 caataggtac gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg
     6241 atgcgcttta ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct
     6301 gtcgtcgttg cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat
     6361 attttaccgt ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt
     6421 cctgagaccg tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca
     6481 ggaggcttgt tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat
     6541 gctttgcaaa ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc
     6601 gtccgtttct tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact
     6661 atcgttcttg ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc
     6721 gtgaacttta tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt
     6781 gtcatcatgt tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc
     6841 attccgggca cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc
     6901 tatgctaggt attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta
     6961 ctcatgcttt ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca
     7021 gttgttcaag aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa
     7081 tggttcaata aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta
     7141 ttgtaaattt atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat
     7201 atgcttgttt cttttagtat ggttataaga ttttttctga caattttatt atcactgata
     7261 ttaacacctg tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa
     7321 agaagaataa ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt
     7381 gttatatcga ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc
     7441 ttgattgctt ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca
     7501 cctaaacaaa aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat
     7561 atacgtattg attccttcac gcttcctttt attggatata tccaactagg ctggcttagt
     7621 tttcccgtaa cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt
     7681 ttggatgggc tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga
     7741 tattttttcc tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca
     7801 agtattatcg gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca
     7861 ggagctctat tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg
     7921 acttttgttt catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt
     7981 tttgctatca ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat
     8041 cttcatcacc gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat
     8101 gcgatggctt tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga
     8161 acgatcgtat tgattattgc catgttgttt gctgcaattt tattatttga attgattggt
     8221 ctgatcaatg agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag
     8281 taccgtacgc aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa
     8341 caaattaaac cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg
     8401 gacactagat aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt
     8461 tgacaacaat tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta
     8521 catcgatatc tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct
     8581 gatagctagc gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga
     8641 gacagttaca gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc
     8701 taagattctt aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt
     8761 tgattacgtt ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact
     8821 ggctgtgttt gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc
     8881 aggtagtttt atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg
     8941 tttcttcatg tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa
     9001 gttattgtac acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa
     9061 tgcaaaactg tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg
     9121 gcgttttttc tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc
     9181 agtcaatctc cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt
     9241 attatttacg ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc
     9301 gtttatcatc atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct
     9361 caagcagatg aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac
     9421 gattattttg aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg
     9481 atgatttttt ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt
     9541 acagctcaag cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca
     9601 ggtgttgcct ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct
     9661 ttagattttt tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag
     9721 atccttcctg tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt
     9781 aatcgatacc gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg
     9841 catttatcca gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc
     9901 aaaaaccggc atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga
     9961 attcgtgcgt tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt
    10021 agatagtatt ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc
    10081 agtagatgat acatgggata tattaactga atacgcagaa aaagataagc gaatccagct
    10141 tttttcgggg cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa
    10201 aggagatcta atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa
    10261 gatcactgac tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat
    10321 tgtggacagc gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga
    10381 aggatggctt aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc
    10441 ttctttaaaa cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat
    10501 tggtttgctg ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag
    10561 acatgattta aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg
    10621 gcgtgttcat ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat
    10681 gtttaaatcc ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga
    10741 aatttgaaat caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag
    10801 cggaacacgt ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga
    10861 caaaccaatg atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct
    10921 gattatttca acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga
    10981 tttaggactg cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt
    11041 tatcattgga gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat
    11101 ttactatggt ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc
    11161 gacagtattt ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga
    11221 gaatatgcaa gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt
    11281 aactggcctt tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc
    11341 agaacgtgga gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact
    11401 atccgtagaa cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt
    11461 gcttgaagca tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg
    11521 tttggaagaa attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc
    11581 acagccattg aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg
    11641 agtccttaca gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg
    11701 acgtctttgg cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag
    11761 aacatggatt gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg
    11821 tccttcgggg gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag
    11881 tcactggagc agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc
    11941 aatgggacgg atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat
    12001 atgcccatgg attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt
    12061 actataatgc agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt
    12121 ggccaattga tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa
    12181 aagaatttga agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt
    12241 ttcttatgaa aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc
    12301 attacgtggt aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg
    12361 caggaaacaa agagaacttg gccggacttc ctagcgatcg cgtagaattg gtcgtaggag
    12421 atgttgcaga tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt
    12481 acgcggcaga atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga
    12541 atatcattgg tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc
    12601 acgtatctac tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg
    12661 gggaaggcga aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt
    12721 cttctacaaa agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac
    12781 gtgcaaccat ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta
    12841 ttcctcgcca agtaacgagt atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa
    12901 agaatgttcg tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga
    12961 cgaaaggcag aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga
    13021 ccgtgatgga attgatctta gaaatgatgg gccaaccaaa agacgcatac gaccatgtaa
    13081 acgaccgcgc agggcacgat ttgcgttatg caatcgattc gacaaaattg cgagaagagc
    13141 taggttggca gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt
    13201 acgaagaaca tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca
    13261 aaaatggaca ataatacaga ggaaatttca ctgtattaat ggaaaaacac atgtgaggca
    13321 ggcgctcgcc tgtctctttt ttaaaataaa aatcaaggag cggataacca tatgttttta
    13381 ttaactggag gaaatggaca attaggtaca gaattgcgcc atttattaga tgaaaaaaat
    13441 ctgccatatg tttcaacaga tgcaaaagac ttggatatca cagatgctga gaaaacaatg
    13501 gcttatatta cagaattaaa acccgatgtt attttccatt gcgcagctta cacagcagta
    13561 gataaagcag aagaagaagc aaaagaatta gatgagaaaa tcaatgtcga tggcacaaga
    13621 aatgtagcac ttgcagcaaa agctgtgaat gcacaattcg tctatatcag tacagactat
    13681 gttttcgatg gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta
    13741 aatgaatatg gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac
    13801 tattatatca tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg
    13861 atgcaacgcc tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga
    13921 ccaacttgga cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca
    13981 tttggtattt atcatttgtc gaacgaaaat agctgttctt ggtatgagtt cgcaaaagaa
    14041 atattgaaag atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa
    14101 gcaacacgtc cgcagtattc agtcatgagc ctgaaaaaga cagaagcgtt aggttttgtc
    14161 ataccaactt ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag
    14221 taatttcctt tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc
    14281 acgacgaaaa gaaaagccaa aagcgaatca ggcagattcg aatttggtgc ttgacctgtt
    14341 tctaaaactc cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt
    14401 aaacggaagt tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa
    14461 aactgatgtg atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac
    14521 acctttttac ctctcacaag ctgaagagga cttttcgttg gtgaacgagg attattcatt
    14581 aaacggacaa aacatgatca ttgcgtataa ttctcctgta aaggatatta cggttatcgg
    14641 aaaaccattc aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg
    14701 ggcgatgaaa ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa
    14761 gaggaaaaaa gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca
    14821 gtggtggttc atgcaacatg tgggcgattt gattttcttt actttgggac tgatgattgc
    14881 tttttatcat tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt
    14941 ggtggttatt ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct
    15001 ggcgtacttg ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc
    15061 atgggatctg ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat
    15121 ggttcatttc tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc
    15181 aggaaaacgg gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac
    15241 aaattggaaa attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt
    15301 ctatcacttt tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga
    15361 tagcgaacaa aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg
    15421 gattaccgtt gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc
    15481 attaagagcc atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc
    15541 ttatatttgg agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa
    15601 tgcatggtct tatttctatg cgattacgca ttcgaaaatg gataattatt tttcggattt
    15661 tgaaaccatc gcagttgctg tgttgggaac agtgatttta gcttttgcat tatttggctg
    15721 gagacgcttg ttttacctcg cttttgtcgc cttagttatc gtatctggct ttttcgtcaa
    15781 tccagtcgta gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa
    15841 aatcgatcaa caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac
    15901 cccaacattg ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg
    15961 gaagaaaatc gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag
    16021 agcttttatc gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt
    16081 cacgttgaat tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc
    16141 attaactgat tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa
    16201 agacggttat atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca
    16261 aactcaggaa aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat
    16321 tagtagaaag gagagtgaag ataattcaag caatcgtaca aaaatttgtc aacaaaatga
    16381 gtgtcggttt cttgcttttg ttgctgacgc tagtcttttt tgtttggaca gcaggggtag
    16441 gttttccagc aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg
    16501 ctattttgct cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg
    16561 ttgcgcttgc ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat
    16621 ttggagactt actgtttcct ttggttattt tattttgttt agtaattaaa tggatcgagt
    16681 ggacaaaatg ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt
    16741 tatatcgtgt atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca
    16801 attcgctttc tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg
    16861 ctttattgat cagtggattt gccagttctt ttgaacgatg gtatgttcga atcatcggtg
    16921 ggatagcagt cgttgcagcc tttttaggta tttgggtctg ccaatctaga ggtgcattag
    16981 tcgcgttgat tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc
    17041 gtgcgccatt tattgcctac acggttacga ttcttttagc tttgccgatt tcttatctgg
    17101 cggctgtctc tgaaaaagtc aatttgttta ccgatcgaga agatatttgg cataaattct
    17161 atcagacgct aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc
    17221 agcgaggaaa ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg
    17281 gattgatagg atttgggatt gctgctttgc tgcttatcct ttttattggt cgcttaacgt
    17341 tgaaagcaga tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga
    17401 tgcagtcatt tatggaggat acattgactt catacccatg ggttccgatt atttatatcc
    17461 tgttggctat ggccgttcat cgatatgatg aaccagaagt tgcggaggaa ttctatgtta
    17521 tggaagaggc atatgacgat atgacttatg atgatacatc ttatgaggaa caaccagata
    17581 gcgctcattc ttccaggatc caacgctatc attgataggc acaaaatacg agagaggctg
    17641 tgacaaccgt cgcagccttt tttggtttat tgcaattgtc tagcatcttc tgtaaaatag
    17701 aaacgttaga gaaaatcttg tatcatagaa aattaagaaa gaaataagaa cgcagctcgg
    17761 taccaaaaat atggtaaaat ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac
    17821 acaaatgttt aaagatttgt atttatttat gaaggacatt tatcagaaca gaaaattatt
    17881 gctacagttt tcgatcaatg acttcaaatc aagatatgca ggatcgtttt taggaatctt
    17941 atgggcgttt atcaatccgc tatttactgt attgatttat tggctggtat ttggtgttgg
    18001 actgaaagca caaatgatcg atgcaaaata cccatttatc gtatatttag ttaccgggat
    18061 gattccctgg ttcttcttct cagatgcatt tgcatcaact acggttgttt ttcgggaata
    18121 tacttattta gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct
    18181 atccaatctg tacacccatt tattcttcat tctgattggc atagctgtcg cgcttataaa
    18241 tggtatatat ccttcagcaa tgtctttaca gctgatttat tatttatttt gcatgtgtgc
    18301 tttcttgaca ggattgactt ggctgacggc atcgatccaa ccatttttac ctgatatcat
    18361 gcaattaatt acgatcgtgc tgcagttgat catgtggaca ttaccgatat tatggagccc
    18421 taatcagttt gattcaggtc ttgtaagcat attgaaatta aatccgctgt actatgttgt
    18481 tcaaggttat cgtgagtcat ttttaagtga agcttggttc tgggagcatg gaacatatac
    18541 tttgtatttc tgggttttca cgttagtaat gttcgtaatc ggctctgcag tgttccgtag
    18601 attgaaacca catttttcag atgtgctgta aggagaatag ctatgacaga atatgcaatt
    18661 agactaaaag atatcacaaa atcttataat atgtacgcaa aaccttcgga tcgatttcgt
    18721 gaagccctca atccttttaa gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg
    18781 aatgtagaaa taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa
    18841 tctacaatgt tgaaaatcat tacgggagta ctgacaccaa catccggcac gatggaaatc
    18901 gaaggaaaaa tctcagcttt gctagaacta ggatcaggat tcaatcctga gtattctggc
    18961 tatgaaaata tctatttaaa tggaatggtt ctaggatttt caagagaaga agtcgatgaa
    19021 atggtcgatg atatcattga atttgctgac atcggagacc atatttatca acctgtaaaa
    19081 acttattcaa acgggatgtt cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc
    19141 gatattttga ttgtggatga agcattggca gtaggcgatc ttgaatttca actaaaatgt
    19201 atggaaaaat ttacagaaat caagaactcg ggaaagacga ttttattcgt ttctcatgat
    19261 atcagttcga ttcgtcgttt ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc
    19321 gaatatggcg atacgatgga agtaacgacg aattatgaaa actttttaaa gaaaaaatca
    19381 gttaaaacag tggatcggaa taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc
    19441 gacgtgacga aagcggaatt gttggataaa gacaaaaatc cggtggatat gctggaacaa
    19501 ggagaaaaac tatacgttaa agtaacttat gaagtaaaag atgatacaat caaaaatccg
    19561 gttttaggaa ttgcgctacg aacagtcgac aatttttatg tatgcggact taatactcta
    19621 ctagatggta tcaagattcc ttggaaaaaa ggagagaata tcttttatct ggagtatgaa
    19681 aagttaggtt tgttagctgg tgaatattat tttgacgttg cggtctttga agaaaatgca
    19741 acggttcctt tggtatataa gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc
    19801 ggagaaggca tcgtggtctt agaccataaa tgggaagaag ggactcatag caatgaaata
    19861 tgattttgaa atggacctag atgaacaaag cagtgtggga aaaattgccg cacagatcaa
    19921 accaggaagt aaagttttag agtttggtcc cggaaacggc cgattaacga aacatttaat
    19981 cggagcaaaa caatgtgaag tgagcattgt tgagttagat aaggaattgt tcgattttgt
    20041 cagcgaattt gctcaagatg gattttatgg tgatatcgaa agttttgagt gggcaaacta
    20101 ttatgctgga cagacatttg attacgtctt gtttgcggat gttttagaac atttggtaga
    20161 tcctggaaaa acattgaaga aagtcagaga atttttgaat gaagaaggag agatcctgat
    20221 tacattccct aacttggcgc ataattctgt catgatcgat ttgttcaata accaattgcc
    20281 gtgggcttct tatggtctcc tagatgaaac ccataattct ttttatactc atgatggctt
    20341 ccaaaaagtt tttgaaaaag cgggattatt cattaatatt gaagattatt tgtacttagc
    20401 tgtaggtgat acagaattaa aatcaactta tgaagaattg ccggaagctg tacgctatga
    20461 tttcaaaatg cgaccatttg gtgaagtcta ccaatacttt ttctcattga tgaaacatcc
    20521 agtagcacaa agttccattg cagagccaca aaattctaat tatgtaaaag tgttagaagt
    20581 aacccagcaa acgaaacaag atgaaacaat ccaacaaata ccattcaata atttcacagg
    20641 ggaaaatgag acactaagtt tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt
    20701 cgcgcaacaa cctagcttta tcgaattcag cgcagaagct gccggagaaa aactaacatt
    20761 tatcgattcg aatgctgtag tcaaaacagt aaatgattgt tatttgtttg atgggaaaga
    20821 attaccagag ttcgttctaa cggatatttc tgggaaagaa gtgactatcc attgtcacta
    20881 tcgctttatc ggggaattga caccaacaat gaaagaattg ttggaaacga ttcgtcccat
    20941 ggctgaagtc acaaagcagc tttctgagaa aaatgacgaa ttagaaagag aaaatcatca
    21001 tcttcttgag gaaaatacaa gactagacca tacgttaaaa acaaccacaa atcgctattg
    21061 cacattgttg gaaagtgatg aatggacaat aaaacataga ctaggacgac gtaagaaaga
    21121 aacttcgaaa aaaatccaag aaaaagaact ttctatatgt attgacgaga aaatctggga
    21181 tgcagaaaca aaaatattaa aaatcatcgg atggggaatc gctaattcgg atcgccagcc
    21241 cttgtcctat aagttatcag ctgaccaatc gccatttttc gaagcaattc ctgtttcacg
    21301 agaagaagtc aatcaagcag aacagctcgc aaaaggaaca gaagcgggat ttgagttacg
    21361 aatcctttgc gagcaagaac gttcattctt agtggaagca gtagctcaaa atggccaatc
    21421 atggatcatt gaaatgtaaa taatgattaa cttataaaga taaggagtaa acgaatcgtg
    21481 caaaaaaaag aaattgccgt atttatcgat caaatcactc gtgaacgggc cactggcgat
    21541 ttgcttattg tgggctgggc aattgatgaa gtgaccaaag aaatccctac gataaaggta
    21601 gaaaaagaaa atgtgattgc tgaagcaaca catgtcgtaa gattggatat caatcattta
    21661 tataatcttg atgttaagac ccaaagcggt ttcaaggtac gtttatcagg gaaaatgaga
    21721 ggaaaagcca tcttagactt ccaaacagcg agacatcaaa atggcatagc tgtgaaatta
    21781 aacggtaaat acccttatga tgacggcatt gaatcttcat gggaaagaaa aaaaagatta
    21841 ctgaaaaaag ggataaatta tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc
    21901 aaattggaat tgaagccagg atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa
    21961 ccagatttga aagagcaaag gaaagaagca caaggatttg cgtaccgtcc attgatttcc
    22021 atacttatgc ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat tgattcggta
    22081 ctggatcaaa cgtatgatca ttgggaacta tgtatcagtg atgatgcttc tactgatcca
    22141 gctatccgaa agtgtctgga atcctatcaa gcaaaagatg atagaatcaa agtcgttttc
    22201 cgaaaagaaa atggacacat cagtctggca actaattctg cattggaaat agcagaagga
    22261 gaatttatag cactcttaga taatgatgat gaattaccgc catttgcatt atatgaagtg
    22321 gcaaaagtgc taaacgtaca tccagaactg gatgtgatct acagtgacga agataaaatc
    22381 gatgcagatg gcaatcgctt tgatccgcat ttcaaagcag actggtcgcc tgatacattg
    22441 atgggaaata attatatctc tcacttaggc gtatatcgaa caagcattgt taaagaactt
    22501 ggcggtttcc gtaaaggcta tgaaggttct caagattatg atttggtcct tcgtgtgaca
    22561 gaacaaatcc ctgcagatca tatttaccac attgaccgag tcttgtatca ttggcggacg
    22621 atcccaggat caactgcaag taatggcgaa gccaaaagtt atatctatga ttccggagtg
    22681 aaagcattga cagatgccct gtctagaaga aacatcaaag gaagcgtacg tcctggcaga
    22741 atttctggat tttatgagat tgcttatgac gtcttgcaag aggatttggt cagtgtgatc
    22801 attccaacga agaatggcta tgaagatcta aaaacttgtg tggattcgat catcgaaaaa
    22861 acgtcttatt caaattatga aatcattatt gcggataacg gaagtacaga tccaaaaatg
    22921 caagaattat ttgcagaata caagcatcag ctgaaagacc gctttatcgt agaattgatt
    22981 gacattccat tcaattattc tcgaattaac aatttagcag cagaaaaagc aaacggcaaa
    23041 tactttttat tcttgaataa cgatacagaa gtgattgagc cagattggat gacggtgatg
    23101 gtttcttatg cacagtttga tcgaatcggc tgtgtaggag caaaactgtt ttatccagat
    23161 gatacaacgc agcatgcagg agtattatta ggaatcggtg gtgttgcagg gcatgcattg
    23221 aataactatg atcgaacaca ttgtggttat ttcggccgat tggtcattga cgtcaattac
    23281 ttagcagtca ctgcagcttg tatgatggtc aaggcagctg acttcaatgc tgttaacggg
    23341 tttgatgaaa cattagaagt agcattcaac gatgttgact tgtgcttgaa agtttatgaa
    23401 ctgggtcgtt acaatgtcta tgcacatcaa gcagaattgt accatttcga atctaaatca
    23461 agaggttatg aagatactcc agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg
    23521 caagataaat ggccagcata tattgcgcat gatccatttt acaatgataa tttaacaaaa
    23581 gacggaatcg gcgacttttc gttgagacca gattaaacta taataaaaag ctgttatgtt
    23641 gtgattcgct cgcacataac ggctttttat agttttttgt caaaaaaagg cagaaggtgc
    23701 cgactcaaaa aaataagtcg aggttgggac agaagtgttt aactccgaga aataagaaga
    23761 aattcacgaa aattgctttt caaatttttg tgaatttcgg cttatttaag aagaagttgc
    23821 ttctgctccc accgtttatt cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt
    23881 cccacgctct tattcatgct cagagcgcca atagatacaa aatcatagcc aaagtttcac
    23941 gaaccctagc tgtcaaagaa tcgatatttt attttaatat aggctatttt aaaataaatg
    24001 gtttaaggta ttcctctaca aaagccactc tatcaattat catgggttta aaataagaag
    24061 tgatggagat aaccagattg ttcttagcgt caatataaag aacatttcca ctattaccaa
    24121 ttgctgaata tatttcgttc tcagaatcta atacccacca taaatagcca tatctcatat
    24181 tttgtaagtc ttctaaagca ctggttctag gtttgaacat ctcgtttatc cacatgggtg
    24241 gtacgatttg ttttccttta tacatccctt tatttaggca taatacacca atttttgcca
    24301 gatcacttgc cgataaacac aaaccatatc ctgccgtccc gatatttagt ggatcacaaa
    24361 accagacatt ggttttagga gacttatcca ttgtaaactg cttatgttct tcagctgttt
    24421 cagcataata attgacatgg tttggtatcc ctataggatc aaatagatac ttattggcaa
    24481 aatcaacagt tgttagctta cttgtctgat acaacaagcc agttaatatg tgtaaacaga
    24541 cagtttgata gttgaattcg ccggttatac cttttcttcc tcccaataaa tctagtgatg
    24601 tttttgtcca atcttcactt gaacagactt ttgtccatgg atcgccttta catttgtagg
    24661 gtgctttcat tgttagcaag tgcttgattg taatagcgta aatagttttc tccccgcgtt
    24721 tcactttata ctctggaaaa taatctaata ctttatcatc gatacttttg attaatcctt
    24781 gctctaaagc aatgccaatc agcaaagata caatactttt cgttgctgac atgatatgac
    24841 aagtatcgtc ttttttataa ccattccatt catctgtata aacttctacc ccatccttat
    24901 atgcgacaat ttgacatata tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt
    24961 tattcataag atattacctc actttcttct ataagcataa tgtcattaaa aatcgatttc
    25021 aattttttcg attcttcacg taatacaggt attcttatgg actagacagc gctttgggaa
    25081 gatggattct ctgaaaaata taaaggttca ctttatgagt ggataaaaaa agcagcaaat
    25141 acttcgactt ctttaattta ccaacattag tgaaaatcga ttcatctgga gatttgtaat
    25201 ttagagtcct tgtcttgatt agaggtgtat aaagcttttt attagacatt tttaaattcc
    25261 attacaattt gcttataaac taggttttta aaggtgacta tggtaagata atagagatat
    25321 tattttgtga ttaggagtgt atatatgaat aaaaatggag aatggagtgc tgcacgacgg
    25381 atattcatta tattaatgga tgttattgta tacaatgcag caatatatag tagtttctta
    25441 ctgaagtttc gtggagagat cccattacga aatttagaaa ctttccaaca ctcagcaatt
    25501 attatttcag tgatttttat tgcgttgaac attttgttgg gtgcatatgt tttttataat
    25561 cgaatgatca gtgacatcgt gtttgtcaca gtcatcggtc aggtattgat gacactaggg
    25621 atcatggtag tgacttttgc gggccgctgg tttgcttttc cacgtcttgt tatcttactt
    25681 tcatttgttc tcggtacgct tttcttaagt atctatcgta ttctcgtata taaactttat
    25741 ttacgcgtaa gtcgagataa aaaggtcgtc attattgggt tagaaaagga tgtcgcacca
    25801 gcaatccaaa attttggttc aaagaaaaac aacaaacata aagtcgaagc agtcattctt
    25861 gataactatt atgcaaacat caaaaatatg attaaagaca tcgatattgt ctatcttgct
    25921 tctcatatcg aagaaactga aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa
    25981 aaattgtttt tgaatacaac atttgaaaac ttgacattga tcaatccaaa tattatgaat
    26041 ttcgaagatg aaagtattat tgaagcatct ggatttagaa tccaacctga atatgaacta
    26101 ttcaaacgtt tgtttgatat catcgtttca cttgttttat tagttattgc ttcaccattt
    26161 atgttattca cagcaatttt agtcaaagtg acatcaccgg gaccaatcat ctataaacaa
    26221 gtgcggatca caaaaaatca aaaggaattt tcaatctata aattccgctc gatgaccgcc
    26281 acagccgaag caaaatctgg accagtttta gctaagagca atgatgcacg agtaacgcct
    26341 gtaggtaaat ttattcgagc tgtccgcttt gatgaattac cacaaatctt caatgtgctg
    26401 aaaggcgata tgtcgatcgt aggaccacgt cctgaacgtt ctttcttcgt cgatcagttc
    26461 aatgcagaaa acccttatta ctacttacgg cataatgttc gtgctggtat cactgggtat
    26521 gcacaagtct atggaaaata tgtctctgat tacaacagta aattacgttt tgatttactt
    26581 tatatcaaaa aatattcatt gatgcttgat ttaaagatct tgctgcaaac catcaagatc
    26641 ctatttgata aaatgtcctc tcaaggactt gaagaagacg aaaatgtgat ctcattggca
    26701 gaaattgctt ttccgaatga aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat
    26761 ccgaaattca gcataatcgt accagtatac aatgcagaag caactattgg tcgaacgatt
    26821 gatattctaa aagaattgga ttatgaagag tacgaagtcg ttttagtaaa tgatggatca
    26881 acagatcaaa cacaggaaat cattcaagaa ctgattgaaa atgatgaacg ttttgaattg
    26941 attactacga ccaatcaagg gcccggatta gcaagaaacg aaggaataag acagtcaaaa
    27001 ggagaatatc tcttattctt cgatgcagat gacacgccag taaaaacgat cttgtcagat
    27061 tatgatcatt tgctagaaaa gagtccaaaa gcggatcttg ttgttagttc gtttgttttt
    27121 cgtacaatgg acgagggaag aatcgtttcc gaaaaagaaa acttagttac agaatgtcac
    27181 tatctaactc acgaagagtt tataaaagac atgtatcagt taatgaatga acagttgatg
    27241 tatgttgtat ggaacaaatg ctatcgtagc gatattctga aaaataacca aattctattt
    27301 aaaacttata atagttgtga ggatcgaata tttaacttac attattatga atactgtcat
    27361 caagtgatca tgaacccaaa agtagaatat atttatgaat ttgaaggtgg gaaggggatc
    27421 actaatcaat accgacctaa caagttcgaa acatttaaag aattctattc attagcaaat
    27481 actgtaacag acgatattaa taaagacggt atggcggcgc ttcttttaaa agggacaact
    27541 tcagttattt tttctattta tagtacctct gcaagaactt caaaagagaa gaaagacgaa
    27601 gcaaagcaga tattgagtga tccagtgatc cgagaagcca agcaaattgc taagacggat
    27661 tcttcagcaa aaaaaataac aaagttgctt tacaatatgc cagctccact atttttatct
    27721 gctgtaaaag ttggaagctt cgttgaagtg aaaatgccag ggttaatggc tgctttgaag
    27781 cgagtatatt agttaatgga ggaaaatata atgaaaagaa ttttgcattt tcagggacga
    27841 atgggtttag gtggagcaga gtctttcatg atgaatttat atcgtaaaat tgatcgtaca
    27901 aactatcaat ttgatttttt gatttatgaa gattatgcag atgttcaaga ctatcattca
    27961 gagattgaaa gattaggagg ccgtattttt gttgtaccta atcctaagaa aaatattttt
    28021 aaatatttaa tagaagtgaa taagttacta aaaaaagaat ctttttctat agttcacaat
    28081 caggtttatt ttggcggggg aattaattta tggttagcca aaaagaatgg aatcagacag
    28141 agaattgctc atagccatgc aactgaagat ggaaaatctc aaaatattgt aatgaatgta
    28201 ttgcgtaaat ttttaaccaa attgttgtta caaaatgcaa cagactacct tgccgttagt
    28261 caagaagctg gtgaaagtct gtttcaaaac catccctttg agatagttca taatggtatt
    28321 gatttagagc tttatagtaa aaattcagaa gctaaggtta ataagagaaa agaattggat
    28381 atctcaatga gtacttttgt tgtgggaaac attggacgat tagagaaaca aaaaaatcaa
    28441 tcttatttgt tagagatttt tttagaaata ctaaaaatga attctgattc ttacttgatt
    28501 ttagtaggtg aagggtcact tagaaataaa ctgaaagcca aagcagtaga attggatata
    28561 gagaaaagag taaaattttt aggagaaaga aacgatattc ccgagttatt atctgtgatg
    28621 gatgtttttg taatgccttc tctttatgaa ggtctgccaa tggttggatt agaagcacaa
    28681 gcagcaaaat taaaattagt cttagcagat actatttcca aagatacaaa gttaacaaat
    28741 aatgtggatt ttgtatcatt agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa
    28801 gagccgtatg ctaataaaat aacacaagaa ctaaagagtt atgatgtttc gtacactact
    28861 gaacaaatgg agagtatata tagtagagat cattaaaaat gttcaagata ttttaggagt
    28921 gattattttt gaaaatatgt tatttgatat tggcatacaa tgatattggc aacttaaaga
    28981 gattggtcaa tcgattgaac gaaaatgcag tttttattat tcatatagat aaaaaaactg
    29041 atatagaacc atttattgaa gcatttaaat catatgataa tgttgaattt ttatgtaata
    29101 aaaaccgtgt taaaatttat tggggtggtt tttccattgt acaagcagaa atcaatttag
    29161 taaagagagc attacaaaat gagaaatatt taaaatatgt attattatct ggtgctgatt
    29221 atccaatcaa agataatgag tatatttata attattttaa aaagaatagt tcagtggagt
    29281 ttattcgtgg aatagatcta gatcaaatta aacataaaga attttattat aaacatatag
    29341 atgtatatca aaaacatgat tatcctagaa ttaataggaa taacacaact gcatttaaaa
    29401 tttttagagc tataattaat cgttgtctga ggatgattaa acttccccca aaaattagac
    29461 accataaatt tgatttgtat catggttctc aatggtgggc tttgtcgaaa gaatgcctaa
    29521 cagaattaat acaaatgtat gaacaaaatc aacaagacta cctgaatttt aaaataggta
    29581 tgtttgcccc agatgaaaaa ttttttcata cacttttttt taattcctcg ttcaaaaata
    29641 aaaatgttat tggaggacca gatttaccac ttgaattgaa aaatattgaa gaaactactt
    29701 tacaaacttc gaaattagca aatattcata ttattgatcc ttccatgaac aaatggtttg
    29761 atgaaaggga tatagatcaa ataaaagaaa gtgaaaaatt atttgtaaga aaagttaaat
    29821 cggattactc tgataaacta ttaaatagaa ttgataaaga aattttgaaa gttggaaata
    29881 tagatgaata gaatagtacc tgttttagag aagagtttat taagtcgaga tgcgtttcga
    29941 gtaataaatt taatcatttg tctgctttat tttatgccta gtacttttaa tgtaactaat
    30001 atcccgatga aaatagcttt tgcttggggt ggaattatag ttttgtatga ggtttttata
    30061 aagaaaaatt tttttaaaat gcgtttttct tggctactga ttggagcaat gattagtttt
    30121 atcttaacta taatcattaa cagagaaaat tttttaattc catctattta taattttgga
    30181 tatttgctga ttactttgat agtaattttt cctgcagact tttcacaaag tgaagaggaa
    30241 aaaaagaaaa aaatggtgta ttttaataat gttttcatta tcataatttt tttagctgcg
    30301 ctaatatcta tctatcaatt tatttttttg atttcttatc atgtcccaac aggaacgcca
    30361 ggattacttg caagacaagg ttttattgaa catcggttat ttggtgtcta tacaagtcca
    30421 aatgttggag ctatgtttgg atatacttct attattt
//
LOCUS       DAZKAS010000020        28508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000020 DAZKAS010000000
VERSION     DAZKAS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.020"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8H7_000755"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8H7_000755"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP8982870.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACG8H7_000756"
     CDS             complement(937..1518)
                     /locus_tag="ACG8H7_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8982871.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8H7_000757"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8H7_000757"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP8982872.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8H7_000758"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8H7_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP8982873.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8H7_000759"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8H7_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP8982874.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8H7_000760"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8H7_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP8982875.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8H7_000761"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8H7_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP8982876.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8H7_000762"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8H7_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP8982877.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8H7_000763"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8H7_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP8982878.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8H7_000764"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8H7_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP8982879.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8H7_000765"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8H7_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP8982880.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8H7_000766"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8H7_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP8982881.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8H7_000767"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8H7_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP8982882.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8H7_000768"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8H7_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP8982883.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8H7_000769"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8H7_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP8982884.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8H7_000770"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8H7_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP8982885.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8H7_000771"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8H7_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP8982886.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8H7_000772"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8H7_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP8982887.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8H7_000773"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8H7_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP8982888.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8H7_000774"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8H7_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP8982889.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8H7_000775"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8H7_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP8982890.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACG8H7_000776"
     CDS             13972..14157
                     /locus_tag="ACG8H7_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFP8982891.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8H7_000777"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8H7_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP8982892.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8H7_000778"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8H7_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP8982893.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8H7_000779"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8H7_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP8982894.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8H7_000780"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8H7_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP8982895.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8H7_000781"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8H7_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP8982896.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8H7_000782"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8H7_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP8982897.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACG8H7_000783"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACG8H7_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP8982898.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACG8H7_000784"
     CDS             18232..18879
                     /locus_tag="ACG8H7_000784"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP8982899.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACG8H7_000785"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACG8H7_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP8982900.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8H7_000786"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8H7_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP8982901.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8H7_000787"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8H7_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP8982902.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8H7_000788"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8H7_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP8982903.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACG8H7_000789"
     CDS             20317..21255
                     /locus_tag="ACG8H7_000789"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP8982904.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8H7_000790"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8H7_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP8982905.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACG8H7_000791"
     CDS             21935..22774
                     /locus_tag="ACG8H7_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8982906.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACG8H7_000792"
     CDS             22750..23619
                     /locus_tag="ACG8H7_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8982907.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACG8H7_000793"
     CDS             23609..24406
                     /locus_tag="ACG8H7_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFP8982908.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACG8H7_000794"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACG8H7_000794"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP8982909.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="ACG8H7_000795"
     CDS             complement(25688..28357)
                     /locus_tag="ACG8H7_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFP8982910.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         9264 a         5298 c         6049 g         7897 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaa
//
LOCUS       DAZKAS010000021        27951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000021 DAZKAS010000000
VERSION     DAZKAS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.021"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACG8H7_000796"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACG8H7_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP8982911.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACG8H7_000797"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACG8H7_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP8982912.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACG8H7_000798"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACG8H7_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP8982913.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACG8H7_000799"
     CDS             1642..2274
                     /locus_tag="ACG8H7_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP8982914.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACG8H7_000800"
     CDS             complement(2276..3181)
                     /locus_tag="ACG8H7_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP8982915.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACG8H7_000801"
     CDS             complement(3266..3925)
                     /locus_tag="ACG8H7_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP8982916.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACG8H7_000802"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACG8H7_000802"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFP8982917.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACG8H7_000803"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACG8H7_000803"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFP8982918.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACG8H7_000804"
     CDS             6168..7253
                     /locus_tag="ACG8H7_000804"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFP8982919.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACG8H7_000805"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACG8H7_000805"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP8982920.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACG8H7_000806"
     CDS             complement(8563..9366)
                     /locus_tag="ACG8H7_000806"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP8982921.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACG8H7_000807"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACG8H7_000807"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP8982922.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACG8H7_000808"
     CDS             complement(10244..11251)
                     /locus_tag="ACG8H7_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP8982923.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACG8H7_000809"
     CDS             complement(11258..11902)
                     /locus_tag="ACG8H7_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP8982924.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACG8H7_000810"
     CDS             complement(12002..12760)
                     /locus_tag="ACG8H7_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP8982925.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACG8H7_000811"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACG8H7_000811"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP8982926.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACG8H7_000812"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACG8H7_000812"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP8982927.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACG8H7_000813"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACG8H7_000813"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP8982928.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACG8H7_000814"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACG8H7_000814"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP8982929.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACG8H7_000815"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACG8H7_000815"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP8982930.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACG8H7_000816"
     CDS             18158..19027
                     /locus_tag="ACG8H7_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP8982931.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACG8H7_000817"
     CDS             complement(19153..20052)
                     /locus_tag="ACG8H7_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8982932.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACG8H7_000818"
     CDS             complement(20217..21041)
                     /locus_tag="ACG8H7_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP8982933.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACG8H7_000819"
     CDS             complement(21052..22347)
                     /locus_tag="ACG8H7_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP8982934.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACG8H7_000820"
     CDS             complement(22363..22842)
                     /locus_tag="ACG8H7_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP8982935.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACG8H7_000821"
     CDS             complement(22848..23876)
                     /locus_tag="ACG8H7_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP8982936.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACG8H7_000822"
     CDS             complement(23893..24999)
                     /locus_tag="ACG8H7_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP8982937.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACG8H7_000823"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACG8H7_000823"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP8982938.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7883 a         6132 c         5041 g         8895 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg c
//
LOCUS       DAZKAS010000022        27744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000022 DAZKAS010000000
VERSION     DAZKAS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.022"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(249..2141)
                     /locus_tag="ACG8H7_000824"
     CDS             complement(249..2141)
                     /locus_tag="ACG8H7_000824"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982939.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISGQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2138..3865)
                     /locus_tag="ACG8H7_000825"
     CDS             complement(2138..3865)
                     /locus_tag="ACG8H7_000825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8982940.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3881..4531)
                     /locus_tag="ACG8H7_000826"
     CDS             complement(3881..4531)
                     /locus_tag="ACG8H7_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFP8982941.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4735..5031
                     /locus_tag="ACG8H7_000827"
     CDS             4735..5031
                     /locus_tag="ACG8H7_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP8982942.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5203..5823
                     /locus_tag="ACG8H7_000828"
     CDS             5203..5823
                     /locus_tag="ACG8H7_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP8982943.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5915..7081)
                     /locus_tag="ACG8H7_000829"
     CDS             complement(5915..7081)
                     /locus_tag="ACG8H7_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8982944.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7093..7446)
                     /locus_tag="ACG8H7_000830"
     CDS             complement(7093..7446)
                     /locus_tag="ACG8H7_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP8982945.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7574..8596
                     /locus_tag="ACG8H7_000831"
     CDS             7574..8596
                     /locus_tag="ACG8H7_000831"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFP8982946.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8615..8776
                     /locus_tag="ACG8H7_000832"
     CDS             8615..8776
                     /locus_tag="ACG8H7_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982947.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9035..9469)
                     /locus_tag="ACG8H7_000833"
     CDS             complement(9035..9469)
                     /locus_tag="ACG8H7_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982948.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9579..11438)
                     /locus_tag="ACG8H7_000834"
     CDS             complement(9579..11438)
                     /locus_tag="ACG8H7_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP8982949.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11439..11579
                     /locus_tag="ACG8H7_000835"
     CDS             11439..11579
                     /locus_tag="ACG8H7_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFP8982950.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11556..12716)
                     /locus_tag="ACG8H7_000836"
     CDS             complement(11556..12716)
                     /locus_tag="ACG8H7_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP8982951.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13023..14447)
                     /gene="araA"
                     /locus_tag="ACG8H7_000837"
     CDS             complement(13023..14447)
                     /gene="araA"
                     /locus_tag="ACG8H7_000837"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP8982952.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14463..15161)
                     /locus_tag="ACG8H7_000838"
     CDS             complement(14463..15161)
                     /locus_tag="ACG8H7_000838"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP8982953.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15118..16779)
                     /locus_tag="ACG8H7_000839"
     CDS             complement(15118..16779)
                     /locus_tag="ACG8H7_000839"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP8982954.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16997..18397
                     /locus_tag="ACG8H7_000840"
     CDS             16997..18397
                     /locus_tag="ACG8H7_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFP8982955.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18649..19728)
                     /locus_tag="ACG8H7_000841"
     CDS             complement(18649..19728)
                     /locus_tag="ACG8H7_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8982956.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19814..20773)
                     /locus_tag="ACG8H7_000842"
     CDS             complement(19814..20773)
                     /locus_tag="ACG8H7_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP8982957.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20785..22296)
                     /locus_tag="ACG8H7_000843"
     CDS             complement(20785..22296)
                     /locus_tag="ACG8H7_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFP8982958.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22318..22881)
                     /locus_tag="ACG8H7_000844"
     CDS             complement(22318..22881)
                     /locus_tag="ACG8H7_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP8982959.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22887..23750)
                     /locus_tag="ACG8H7_000845"
     CDS             complement(22887..23750)
                     /locus_tag="ACG8H7_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982960.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23764..24660)
                     /locus_tag="ACG8H7_000846"
     CDS             complement(23764..24660)
                     /locus_tag="ACG8H7_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8982961.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24835..26124)
                     /locus_tag="ACG8H7_000847"
     CDS             complement(24835..26124)
                     /locus_tag="ACG8H7_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8982962.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26096..27613)
                     /locus_tag="ACG8H7_000848"
     CDS             complement(26096..27613)
                     /locus_tag="ACG8H7_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFP8982963.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8106 a         5848 c         4792 g         8998 t
ORIGIN      
        1 ctatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg
       61 aagaggttgt aaaatcagcg gtttgacctg agtattagag atacaaaacg gaaaaatagc
      121 ttctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga acaccgttta
      181 ttcggaaaaa gggactgtga cacgactttt gtcacagccc ctaaaaaaaa taataaattt
      241 gtactattct atgaagcttc ttcttcagaa aactgactgt tataaagatc tgcatagaat
      301 ccgtcttttt ccatcaatgt atcgtggttt cctgtttcta cgatcgaacc atgattcatc
      361 acaataatgt tgtctgcatc gcgtattgta gacaagcggt gagcaacaac gaaacttgtc
      421 cggttttcca ataggcggtt catcgctttt tgaatcagta tttctgttcg tgtatctaca
      481 ctagatgttg cttcatcaag gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt
      541 gtgattagct gacgctgccc ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta
      601 tatccttctg gcaatttacg aacaaaatcg tctacgtgag cagctttagc agcctcaatg
      661 atttgttcgt ctgttgcatc gtcgttccca tatttgatat tgtcatagat acttcctgta
      721 aacagccaag tatcttgcag taccattgaa aactgtgcgc gcaattcatc acgtgaaaga
      781 tcacgggtat ctacaccatc gtaacgaata ctacctccag aaacatcgta gaaccgttca
      841 agaaggttga tcagtgtcgt cttccctgca ccagtcggac ctacaatagc gaccatctca
      901 ccaggtttga catccaagtt aaaatcagtc attagcaagt tatctggtgt gtagccgaat
      961 tgaacatttt caaaacgaac tttgtatggt gtatcttttt cttctggaag gccagatttt
     1021 tcattggaca tctcttcttc atccaacact tcaaataccc gttcggctga agcaactgtt
     1081 gattggatag tgttcatcaa acttgcaagt tgagtgattg gttgagagaa ctgtgttgta
     1141 tattgcaaga acgcttgaac atcccctaat gtcatgttcc catttgcaac ttttactccg
     1201 cctagtacag cgacgaacac atagcctaga tttttaacga aattcatcaa cggcatgatg
     1261 attgccgaaa taaattgagc tttccaacca gcggcataca gattctgatt ttctttttca
     1321 aatacttcct ggtctttttc agtatgattg aatgttttaa caatggtatg accaccatat
     1381 gtttcttcta cttggttatt caataaacct aaactttttt gctgatctgc gaaaaacact
     1441 tgtgatttcg gcgctacgat catcacgatg atcaagctta aagggatcat caataacgcg
     1501 actaacgtca attgcccgct gattgaaagc atcatcacga tgaccccaat caatgtgact
     1561 gcactagtta caaattgagt caaattttgc tgcagcgtgc ttgcgatgtt atccatatca
     1621 ttgattgctc gagacatgat atccccattt gtatgtgtat cataatagga aatcggtaca
     1681 cgattcattt tggcttccaa atcatgacgt aattcataaa ctgttcgttg ggaaacacgt
     1741 gtcataataa attgctgtaa aaagttgaat acagcagaaa tcaaatacat cacgattact
     1801 gtcgcaatga tctgcgcgat tttatcaaag tctatcggaa attttgttac ttgttggcct
     1861 tgttgcatct gttgagcgcc agccattaca cctttgaata tttctgtggt tgctttcccc
     1921 aagacctttg gtgtttggat ttggaagatc acggctgcta ttgctagtac aaatacagcg
     1981 atgatcgcaa ccatacgttt agacatatag ccaaacaatc ggcgtaccgt tccccagaaa
     2041 tttttaggct tttcaccttt tacaccgaga tttctgccag gtccaccatg tcctggaccg
     2101 cctgctggtc catgagaggg acgtgtgttt gttttactca tgctaagtcc tcctctctta
     2161 gttgtgaatg aacgatttct tgataagttt cgtttgttgc taatagttct tcatgtgttc
     2221 ctttgccaac cagttttcct tcatctagca caaggatcat atctgcatcc attactgtac
     2281 ttactcgttg agcaactaaa acggtgatac tttctttcat attcgttttc aatgcttggc
     2341 gaagattggc atccgttttg aaatccaacg ctgagaagga atcatcaaac acatagatat
     2401 ctgctgtttt gactaatgcc cgtgcaattg ccagacgctg tctttgtcca ccagagaagt
     2461 ttcctcctcc ttgttccaca tggctatcta agccatcttc catctctgaa acaaaatctt
     2521 tagcttgtgc gatttctaat gctttccaaa tcatttcatc tgttgcatcc ggcttaccat
     2581 actgcatatt gtctcggatc gttccagaga acagaactgc tttttgagga acaaagccca
     2641 ttaattctcg caaattatat tgagtcatgt cactaatgtc cgttccattt atttggatcg
     2701 aaccagattc gatgtcatat aatcgaggga tcaaattgac taaggtcgtt ttacctgaac
     2761 cagtcccccc tatgattgcg acgatctcgc ccgacttggc ttggaaatca atatcttcca
     2821 atgcaagttt ttctgctcca tgatatcgat aattgacatg attgaaggcc aatgttgcgt
     2881 tttttccagc aaaagaaact gttttaggat tttcagggtc atggataccg attttttcat
     2941 ccaacacttc attaatacgg tcagctgaag cttgtgcacg aggcaccatg ataaaaatag
     3001 cggaaagcat cataaagctg atcaagatct gcatcgcata agtcataaat gcaatcagat
     3061 taccgacttc caaagtcata tctgctatgt agtgtccacc aaaccaagtg attgctacgt
     3121 ttgtaccgct catgatcaat gtcatcattg gcatcatcaa tgcgacgatc gtattaactt
     3181 tgattgccgt atttgtataa tctttattcg ctaaatcaaa acgattttct tcaaagcgtg
     3241 tcttatcgaa tgctcgaatc actctgaccc ctgtcaatcc ttctcgaaat accaggttta
     3301 aacggtcagt tttcttttgc atacttttga ataatggaac agcaaagtac atgattccgc
     3361 cgatcaaaat aatcattact gggatgacaa ctaagaagat ttttgtcaac tgatgatctt
     3421 tttgataagc aagtatgctc gcgccaatca acgtgattgg tgcattgatc atcatacgta
     3481 aaaacatttg tgtaaccatt tggatttgag tcacatcatt ggtcgttctt gtgatcaaag
     3541 aggccgttcc atatttatca aacgcatctt ttgtcagact ctctgatttt ttatagacat
     3601 ccgtacgcaa ttgcttaccc agtttctgtg attcacgagt agcaaaaaat gtatttgcga
     3661 tagcagctaa gatactgatc aaagaaaagc cgatcatcac aaaacctgta tgccaaatat
     3721 aatcgacatc tccttgcgct acccctttat caataatatt ggaggtaagt gtcggcaaat
     3781 ataaatctgc cagtacttgg atcaccatga aaactgcagc tgcaattgct gaagtcaagg
     3841 agatccgttt tacgattttt agcatctagt tcccttcttt ctatttatat actccgtttt
     3901 tcagccaaga aagtttcaaa ttgaattcac tgattgcttg ctctactgag taatcttcag
     3961 atgttgaaaa taagcgatac gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt
     4021 ggatcagtaa ttccaattca tgatcatttt ttactttcag cttcgtccga tcgatcaatt
     4081 caagcaactc ttttgcatct tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc
     4141 gactcgtatg aggagcgact tttcttgagg cacggtaatc catgttcata aacaagttac
     4201 gataaaaaga agcattctcg ccttccaaaa tctcataaat cattttagaa aaataggatt
     4261 cgaatccttt gaatagatca ccatcggatt ctttcattag ttgtaacata tctttagagc
     4321 tgtcacgtct cattgtctca aagtaataaa aataaagatc ctctttatct tcaaaatact
     4381 gatagaaact gcctctaggg atatccgcta atttgacgat attagcaatc gaagcttctt
     4441 ttaagggtac tctagaaaac tcgattctcg cagcctcgat tagtcttttt tgtttttctt
     4501 taggcaaatg gaaaaatgtc tgtgtcggca tatggatcct cctttctttc aagatgacac
     4561 attgtcatat gacgctgtgt cattataaca cctcctacct gttttgcaag acatttgcat
     4621 agtttttttc attatcatcc gatacattaa aaaagtgata agaggaatcc ttccatttga
     4681 attaatgaga gttgcccatt aaaatagcca catactaaac aaaggatgag ataaatggcc
     4741 caatcaatta gtgtccaaga cttcaataaa ttgcctctcg atgctgacag catcgtttta
     4801 gatacaagaa ataccttaga ttacacaaaa aaccacttaa gccactcgct ttctatcccc
     4861 gctgctatct tgccaagaca attaaaaaat ctagatccaa agaaacttta ttatattcta
     4921 agctatacag gcaggcgatc agaagcatta gccgatcaac ttactgctaa tggttttcgt
     4981 gctgtttata tcattggtgg gatggaagct cttcagtcga gtgttgcttg aatattgctt
     5041 gttagattca aaagaggctg tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct
     5101 ttttttagtt atttattctt caaccttatc aatatgactt gatagccttt tctttttctc
     5161 atagttataa caatcatagc aataaaagga gaaggtaagc atatggcatc tgttttagta
     5221 gtcaaaggtc atcccctaac tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt
     5281 ttaacaagtt ataaagaaaa tcatccagat gatgaagtga ctgttttgga actttatcgc
     5341 gatgatattc cggaaatcga tgaagaatta ttatcaggtt gggaagcatt acgtgctggt
     5401 gcagaattca cttccttatc agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg
     5461 actgatcagt tccttgctgc tgataaaatc gttatcgcaa acgccttatg gaatctaaat
     5521 atcccaacaa aattaaaagc ttggttcgat accgtcaatg tagctggaaa aacgttccgc
     5581 tatacagaaa acgggccaga aggattagtg acaggtaaaa aagcactgca catccaatct
     5641 aatggcggtg tatacaacgg acaagacttt gcctctcaat atgtaaaagg tattttaaac
     5701 tttgtaggca tcgatcaagt agatcagcta ttcatcgaag gaatcgatta tgaccctgac
     5761 cgtgcagatg agttgatgca aaatgcatta gataaagctg ctgctttagg taaatcattt
     5821 taaaaattga gataaatgca tacaaaagag acagaggaaa aaatcttcaa agcagcattt
     5881 gtaccttgga tattttccca tctgtctctt tttgttattc tttctgcttt ttcaacggat
     5941 tttcttcata aagagaagag attgttgtgt accattcaaa aacatgagta atcactactt
     6001 tagctgctgc ataaacagga atccccaaga tcacaccgat gattccgaac aatttcccag
     6061 aagtcagcag aactaataaa atcgttactg gatggattgc taactgattc cctagtacta
     6121 atggagagat caagcgtcct tctattgtct gctctactgc aaaaacgatc agtactttga
     6181 tgatcatgaa tggacctgct acaatggcta aaaagatagc cggaatcatc gctaaaaatg
     6241 atcctaagta agggatcagg ttcaaaaaac ccgcaataat ccccaacgtg acagcatact
     6301 ctaaaccaat gaccgcaaaa ccaatcataa acatgattgc cacagcaaaa gccacagtta
     6361 gttgtcctct tatataagaa gaaacttgac tattcatttc actcagcaca ttcaacgtcg
     6421 gtttttgcat gcgtgtcggt aaaaatttga caaaataagg tgctaattgt ttaccgtctt
     6481 tcaatagata aaataagata aacggcatcg tcagcagagc cactaaaata gtcgctaccg
     6541 cgccgacaaa acttccaaga ctctgaactg tactcttaga tatatcttgg atcatatcgc
     6601 ctgccgaatt catgaactta tcacctgctg tttccaactg ttcgcgaaat tggttgaaca
     6661 gcgggtcccg caaaatctcg gttaatttat tatcaatcgt atctacatat tgagggaagt
     6721 tactgatgaa gctcactgtt tgttcctgaa tcttagggat gatcactacg ctcccccatg
     6781 cgattagtgc aacaaccaaa acaaacagcc caatgatact gtaaagcctt ggtacttttc
     6841 gcttttcaaa gtaatcaact accggattca tcaagtaata caagattcct gctaaaatga
     6901 taggcagacc tacgattgct aaaaattgcc agattggtgc aaacaaatat gaaacttttg
     6961 taaataaaaa gacgatcagc aatattaaca atacaacaag taaggctgtg actacttgat
     7021 tatttaaaaa ccagcgccaa aaccacgata ccggtctgtt cttcttattt tttccttcca
     7081 taattcctcc tgttagttat aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg
     7141 cggatgaaga tagattccat ttgcaatttg tccttcttct ggacaagcat caaaaatcaa
     7201 tgatacgtga gctactgtct ctaaattccc attggccatc ggaccgacat aatcgatcgt
     7261 atatacctga tcatcaaaag caattgttcc gcctgctttc aaagaaaaag gttcgtcttt
     7321 tgatacttct tgaatcaccg aatattttcg taaggcagct gttgctgttt tatcgaacaa
     7381 aatcagcatg ggttcttcct tgtttattgc ttctgtccca atttctgtaa tctttgcctg
     7441 aatcattgca ctctctcctt taattgcttt caaaaacagt ataacacatt ctcattcttt
     7501 agccgtgtgg aataattatg tgatagaata aaaacgaaaa ctatttcatt acaattagga
     7561 ggaatcagct catatgatcg aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa
     7621 aggaatctat acgatcgacc ttgatacaga aaaagaagaa ttagccaacc tttcattagc
     7681 tgttgaagaa aatagcccta cctatgtagc aaaaagtaaa gctggcaatc tttacacagt
     7741 tactagtgtg gatggattag gtggtgccgg tgcgtacgat aaggattttc attttttgaa
     7801 tgcggtaaca gagtctggcg ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt
     7861 actatacggg gcgaactatc ataaaggtga agtaaatgtc taccatatat taggagatgg
     7921 cgaactgaaa gcagcagatt caatattcca tcaagaagcg acaggtcctc ataaaaacca
     7981 agatcatgcg catgtccact atacggatct gactcctgac caacgattag tcgtatgtga
     8041 tcttggtaca gatcgtgtgt atacttatga tgtgtccgaa aacggcaaat tgaatcttgt
     8101 aactaccttc actgcagagc caggaacagg ccctcgccat cttgtctttc atccaaatgg
     8161 acaattcgct tatttatttg gtgaattaga cagcacagtc cgcgtgcttt cttataataa
     8221 agaaacaggt gcttttgaag aattacagaa aatatcgacc ttgccagaag aattcgacgg
     8281 tgaaaatgga ggggcagcta tccgcatttc aaatgatggc aagttccttt atgcttctaa
     8341 ccgaggacat aactcgattg ctgtattcgc agtttttgaa gatggttccc acattcaaaa
     8401 caagcaaatc atctcaacag aaggggattt tccccgcgat ttcgcactag atccaagcaa
     8461 taattttgtc gtgtgtgcca atcagaacac cgataatctg acattatatc gccgtcatcc
     8521 tgaaacagga ttactggaat tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt
     8581 ttattttgat cgttagtaag aaaaggagaa tgaaatgaat cagcaagacg tattagacag
     8641 actacgggaa gaactaaata ttcctttctt tgatgggaaa atcgaagata aagagtatag
     8701 cgaagaggaa tatcagaaac tcaaagccga tttacagaac tactttgaac aatatgtaca
     8761 gaatgtagaa aactaatcag ctttccatac tatctgaaaa gaacatcagg acaagacttt
     8821 tccgtctatc ctgatgttct tttctacaaa taaaaaaccg ccgagatggt tagactcgac
     8881 ggaaaaggag tatttactcg attttgagta agtacaaggt tgttgttggt tataggtatg
     8941 tcattatcat aaaggatttt gatcactcta tcataaaggt aatattacat ttatattaga
     9001 taactctagt ttttattgtt atcaaagatt tcttttattt tttcaacaac aaattagtca
     9061 ttttttggat ggcagctgtc aattggtctg tcgcttggtc ttgttttgct tcagatgtat
     9121 tgaatagatc ttcatctacc cctgattcaa gatcgatact ttggatcatt tcttcacagc
     9181 ctgctacata tgaaagataa gctttttcaa aatttttatg aatgcccatt gcttttgctg
     9241 gtggacgcaa agtactgact ttcttaagca ttgcccgata tttttttgtt ccttcttcaa
     9301 ataacaggat gatctcttca agagtcgatt tgtccagttc acttgttttt ccttcatcaa
     9361 ttgcgtttct gatcacttca tatttcggat gcattccttc tccgattgct tctgtttcct
     9421 tgacgatttg atcgatggac tgcacataat agccaatatt cggtaacatg ttcgttttcc
     9481 tactttctcc attgttgtta ttatttattc tacttgtttt tatcctcaaa tgacaagtac
     9541 ttgaaaaaat ttaatggttt ctatacagct cgccattctt attcgtgctc aaagaaacca
     9601 tctgctaaac tatattgttt ccctgcgaca atgtcatatt ggatcaattg atattcgtcg
     9661 taaatctctt gttgcgagcg attgaattgc gcttctttat accactgtcc gttttgatag
     9721 tacagttctc gttcaaacgc cggaagttcc tgttctaatg ataaaagcag ctgataaaag
     9781 ccatttgttg tctgattcgt ctgttccatt agattcgctg caaaataaaa cggactggtg
     9841 attgcatcgt gagtcgtttg cttttctaat tctcctttac tgtcgaacat aagaaattgt
     9901 gtttcatgca atgtttgttt gtcatttttt gcttggatcg tatctgaata gatccctgga
     9961 agatgatccc cccaaaaaac aactaaagtc cttctagaaa gattcttcaa ttcttccgtg
    10021 aactgcttca gtgccgtgct gctataggaa atatcttgca gataattctc taagtcaagt
    10081 gtcccacttc catctgaagt ttttgcagga tagtctagat tatcatactt tccatcgtag
    10141 ggcatatgtg tctgcatcgt gactaaatga atgaactgag gtgttttttc ttccttgaga
    10201 agtgtcatta cttctttgta agctgactca tcagaaatat atggattatt ctctatcgta
    10261 tccgtatagg tcatcgtacg ctcgctgatg aactggtcaa atcctaatgt ctgataaaca
    10321 tcctctcttt tatacatcga agtattgtat ggatggatcg ctgtcgtctg gtatgattgt
    10381 gcgtttaacg ctgaaacgat agaaggaagc tgatccattt ttggtacaag catcgtgtaa
    10441 ggggtggtga gttgcgcatt aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg
    10501 atatttgctg ttccaccgcc atagttttga gaaagcatat tgccactata tgtttgatca
    10561 gctacttcat aataatctgc caatggctca ccagatactt ctacgccgtt taatcgacta
    10621 ggatctgaga aactttcact catcacgaac acgatgttcg gcgactcttc ctctacagtt
    10681 ttattttttt catcggctag cttctggtat ttttcagtga tttctttgat cttttctttc
    10741 gaatatcctt caggttcatc cattggttct accttcagat tgtacaaaaa cccaccgata
    10801 aaaccagtgt tatagtagtt cattttctgg ctgtaaggta tccatagtgc cgttttatta
    10861 tatgcttttc gcaacaaatt atcgggattg ttgaaattac tgatataccc tagcagtcca
    10921 attgccacaa gcagtgtcgt taatcggatc aattggaatc gccttccttt tttcaggctt
    10981 cgaaacatgt accagcaaaa caatcctatc acggttcctg cggcagctaa gatcactgta
    11041 aacagcatag tgccggtcat atctttcagc aatccgattt cagtgatcat cttcaaatcg
    11101 tctggataaa tcggttcttg ccgataactc attttcatat aatttgcaaa acctaataca
    11161 ccgatggcta cgacatacgt aagggaacct aaagggacag atcctgctag tgcaatcaga
    11221 aaaataagaa taatcagcaa gacgagacag gctaagaaga atttttccgt atgccaagaa
    11281 aaagcaaatt tcaacgcaag gtctacggat agatcatttt gacaccactg caaaaataaa
    11341 ttactaaaaa caacaagaaa aaagatactg atgattgctg ctgttccttt taaaatcttt
    11401 ttctgtttgg tcgaaagtct gcttatatat cttttcaaat gcttgctcct tcatttctat
    11461 caataaaaga atgttgcttc tcttatttta gttgtacgaa attcgaaagt aaagaacgga
    11521 aagaagaatt aatgaaatcc aaatattctc tttctttatg gcctttctgg tttctttaat
    11581 aagcggacct ttcgtactcc agaaattttt tctagctgtt gtttcacttg attgagctgt
    11641 tcaaaatcag tactgtctat gtcaatcaat atgtagacat gctcattctt atggttacgc
    11701 gatatatctg cgatcgctag ctcgttctca ttcagcagag aaaatatctc tgctaatata
    11761 ttggttttct tttgataaaa tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg
    11821 gatggataat tgaccgcttc tctaaccgta ccgaacaaaa gataatcttt taaggcttca
    11881 actgccaaac gtccgctgtc tgccaaagct tcctgagtcg atccccctag atgaggcaac
    11941 ataagtatac ggtcattttc taagaactct tcttctggaa aatcagtcaa gtatttagcc
    12001 agatgttgtt tttccaatgc ttgcataaca gcatatttat caacaatttc acttcttcct
    12061 acattgataa gtacagcatc ttttttcatt ttttctatat tcgcctgatc gatcaatcct
    12121 tttgtatctt ctgtcaacgg cagcatgact acgatgtaat ccgaggtaga aagaactgtt
    12181 tctaaatctg cttgccgaac ataatccaac tgggcatcgc ggatagaata acccaagaca
    12241 tccatcccta agctatagca acttagcgct acttttgtgc ctatcgctcc aagaccaaga
    12301 agaccgatgg ttttgccttg caattctcgt cccacataag cacttctttt gttttctgcc
    12361 tgttctaaaa tattaggacc tgttagtgtt tgtaccatac gacttgcttc gattatcgga
    12421 cgggaagata agagcaaaca gcacaaaaca agctctttta ctgcattagc atttacacct
    12481 ggtgtattca tgacgatcgt cccgttttca gaagcttttt caacgttgat cgtgttcacc
    12541 ccaacaccag ctcttgaaat tgctagcaaa tctggtgtaa gccattcatc tttcactttc
    12601 gtactacgaa taactattcc ttcggctggc aaatcatcta ttattttgaa atattcccct
    12661 tgtaactttt ctatctcttg ttctggaata gcatcaatca aatgaacgtg atacataaaa
    12721 ccccctcctt actaacaagt atctcgaaat ttctgcaatt agacaactgt ttaccatttg
    12781 acttcctaga agcaaaaaga gcagttagag cagagtaggc tgagtgataa aaataaggct
    12841 atgacaagtt actgccataa gccttatttt cgagagcaaa atattgaggg tgagatatct
    12901 tgataaatgg tgttccgtta gctgacattg ataataagac taaatttcaa aaaagttgaa
    12961 aagcaaattt cgtcaatctc ttcttatttc ttaaaactaa gtggcttttt cacaacctct
    13021 ttttatctaa taaccactgt ttccaactca actaattttg cccacgtttc aatttgatct
    13081 gttgttagat ttagcgagac aacggtatga tgaccgccac ctgcctgaat ccatgaatgg
    13141 acaccttcat ggaaattcgg tttagttttc cataacacac gtgcaacagg taaattaggt
    13201 gcttcagtcg ttggttcaaa ggcttctact tcattgatca gcagtttata atgcgtaccg
    13261 aaatctgcca tcgatacgac taccccttct cctgctttcc catcaaatac aagacgcgcc
    13321 ggatcttctc tatctcccat tgataaagga gaaacaacga ttcttggttt cgtcgcagca
    13381 agcgtcggat ctacttccat catatgggat tccaagattg cttcttggcc actagctagc
    13441 tcgtaagtat aatcttccat gaatcctgta tctttccctc gagccatgat tttcagcaac
    13501 cgatcaatcg ctgcagtttt ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc
    13561 aaacgttgta cagctagtcc aggtagttgt ttcattccat acaggtcttc aaaattagtc
    13621 gtaaaagcgt tatagcctcc ttcttctaag aaacggcgaa taccgatttc ctgctgagct
    13681 tgtactttta catgttcctc ccatgtcttt tcttcataat caccatagtc gaaggtatac
    13741 aattccttgt actcttcaaa taattgatcg acttcttcgt ctgatacgcg atcgattact
    13801 tgtacaagat ctccgatacc aaaataatca acagtccaac caaattgaat ctgtgcttcc
    13861 actttgtctc cttctgtgac acccacgttt ctcatgttgt caccaaaacg agccactttt
    13921 attccaaaac tttcattata ggctactgca acatccatcc aatcagcaat ctctttttgg
    13981 atctcaggtc gtttccagta gccaacgacg attttatttt gtttatttaa tcgagcattg
    14041 atgaacccat attcgcggtc accatgagcg gattgattta aattcatgaa atccatatcg
    14101 atcgttttcc aaggaatgct ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt
    14161 agaagctttg tcccgcggat ccacattttt gctggtgaga aagtatgcat ccaagtgatc
    14221 acacctgcta cttcttctcg ataattgacc tctttcatga tttttgtgat cgtatcagca
    14281 gtaactgcta attcttgaag gcggattgga taaggcaatt ggccattttc attcagttca
    14341 tcgaccattt cttgtgcatg tttctttact tctcgcagtg cttcttcccc atataaatgc
    14401 tgtgatccta caacaaacca aaattctttt tctccaatat tcaacataat tagccactcc
    14461 ttttatttgt tttgtccgta atacgcattt tttccgtgtt ttcgtaaata atgtttatct
    14521 aaaatccgtt gaggcagctc ttctgaaaat gaattgagct gtctggtaaa taaattcatc
    14581 ttgcatactt catctagtac gactgcgttc attaccgcac tttgcgcatc tttcccccaa
    14641 gtaaatggac cgtgtccgtg taaaagtacc cctggaattg ctaagggatc aattttgcgt
    14701 tctttgaatg tttctatgat aacttttcct gtttcatatt cgtacccgct gtcgatttcc
    14761 tgttgtgtca aaaatcgagc acaaggtacc gatccataaa aagtatctgc atgcgtcgtc
    14821 cccatcgcag gaacatccaa tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta
    14881 tggacgattc ctccgatttg aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc
    14941 gaagagggat tgagtttacc ttcaatgata tgattgtcca agtccacaac gaccatgtcg
    15001 tctgctgtca tctcttcata actgactccg ctaggtttga taacgaaata tcgtgtttca
    15061 ggatcaaaag cgctcacatt tccccatgta tatttgacga gtccgtgttt aggcaagtca
    15121 agatttgctt ggtatacttc ttctttcagt tgttccagca ttttctatca ctcctttttc
    15181 ttgcaattga tgaatcgctg ttttttcaat gtcgatcccc tcacggtaac gtctcataaa
    15241 tatctgatag cctgctattt cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc
    15301 aaacacgtct tctgccaaga agttttctaa agacttccca tgtcgatcca tcatataggc
    15361 tgctaataac gcgattcccc acgctccacc ttctccggct gtgtccatca ctgtaactgg
    15421 tgcctccata gcagatgcta gaatccgctg tccaacttct ggtgttttaa aaatcccccc
    15481 atgccccact aaacgatcaa tttggacatt ctcagatttc aatatatcca ttccgattct
    15541 cattgcacca aaagcactgg ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct
    15601 acctggtgta cgtacaaata atggccttcc ttcatttact cctgtaatgt tttcaccgga
    15661 aaaataacca taagaaagta atcccccgca atccgtatcg ccttctagcg ctttgttgaa
    15721 taataccgtg aataatttat ctctgcttat ctccataccc aaagaattgg taaattcttc
    15781 aaaaatactg acccaagcat tgatatccga agagcagtta ttcgtatgaa ccatgcctac
    15841 tagttctcct gaaggtgtag tgaccatgtc gatttccgga tggacatttt ctagttcttt
    15901 ttctaatacg atcatagcga aggcggaagt accagccgaa atgttccctg tccgtttggc
    15961 tacactgttc gtagcgacca taccggttcc tgcatcccct tctggcggac aaaccgggat
    16021 tcctggtctc aaatttcccg tagggtcgat cagataagcc cctgattcgc ttaacttgcc
    16081 agcttcttct cctgctacta gaactttagg caaaatgtga tccaagtcaa tcgctacgtg
    16141 cgctttttcg attaactgac caaaaatatt tttcatttct tgatggtagg ttttcgttga
    16201 aggatcgata ggaaacatgc ctgatgcatc tcctacgcct agtactttct caccagtaag
    16261 ctgccaatgg acataacctg caagcgttgt caaaaaatta atttctttca catgttcttc
    16321 tttattcaaa atagattgat acaaatgagc gatactccat ctctgcggaa tattgtattg
    16381 aaatttttcc gttaatattc gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa
    16441 tggtactagt aactgttcct tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa
    16501 cccaatcgac ccgattgtcg taagattctc gccatagatt tcgaatactt ctgctgccat
    16561 ttttcggtaa ctgacgcgca gtcctttcca aatatcttct aatgaatagg tccagatacc
    16621 attttctagt ttgttttccc actcataact accagcggca ataggagtaa aagatgaatc
    16681 aatcaataca gctttgatcc tagttgatcc aaactcaatc cctaaagatg tacgaccatc
    16741 tatgacatct aacatacttt gcgcatttac ttccttcata atgatttaac tccctccttc
    16801 tcgtcaaacc tgatcaaacc gtctttttag tagcgcttac aatgattatt gtatactttt
    16861 gtacgtacat gtcaagcggt tatttacaag aatacaataa aaaacacttg ttttgtacgt
    16921 acataagtta taaaatgaaa ttgcttacaa aatctgatca accgccttct cgaaattcta
    16981 gaaggagaga atacttatga aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt
    17041 tggcgcattt ggcggcattc tatttggata tgatattggt gttatgaccg gcgccttacc
    17101 ttttttacag catgattggg gtttggcagg taaagcttct ttgataggat ggatcacgtc
    17161 ttcggtcatg ttaggtgcga tccttggcgg ctctttatct ggtcttcttt ctgataagct
    17221 tggacgacga aaaatgattc ttctttctgc acttattttt atggctggtt ctgtcctttc
    17281 tgcatctgcc cctcacaatg gttcttattt tctgattgca gcaaggattc ttctaggttt
    17341 agctgttggc gctgcatctg ccctagttcc tgcttatatg tctgaaatgg ctcctgctag
    17401 actaagggga agattgtcag ggatcaatca agtgatgatt gcttctggca tgctcctttc
    17461 ctatgttgca gattatttgc taaaagtgtt gcctgaaacg atggcttggc gcgtgatgtt
    17521 agggctcgct gctgtccccg cgctgatttt atttttcggt gttttagctc taccagagtc
    17581 accgcgtttt cttatgcaat ctggccgctt ggaagaggca aaaagagtat tgaactatat
    17641 tcggacacca aaagaagctg aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga
    17701 aaagacaacc ggaacttctt ggcatacttt gtttttagaa aaatatcgtt ccttagtcat
    17761 tgccggtatt ggtgtagcag tattccagca atttcaagga gctaatgcta ttttttacta
    17821 tattccgctg atcgtagaaa aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc
    17881 gatcatccaa ggaatcattt tagttgctgg ttctttgctc tttttggtta ttgcagacaa
    17941 gttcaatcgg cgaactttat tgaaaatagg cggtagcgtg atgggacttt cattcatcct
    18001 accagctgtt ttaggaactg tactggatgc tcatacaaat tcgttgttga ttctcttgtt
    18061 tttatgtatt tatgtcgcat tttattcttg cacatgggct ccactgacat gggtaatcat
    18121 aggcgaaata ttcccactag ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa
    18181 ttggattggt tctttcctag ttggcttgct ttttcctgtg atgactgctt ctatgtctca
    18241 agaaatcgtt ttcggcattt ttgggatcat ctgcttttta ggggttttat ttattcaaga
    18301 gatcgtacca gaaactcgtg ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa
    18361 aacttatcct aaacgtattt ctatccatca ttcttaaaga ggttgtgaca gaagtggtca
    18421 gcttcaagaa ataagaagga accgcttagt ttccagtcac aaggaacaat ttcaaaaaac
    18481 agcttctcat gtttgatgaa tattgtgact tgtgaccagt tggtaggaga acaccgttta
    18541 gtggagaggt ggtgaaagaa acgttcagcc ttgagcgatg acaaaagctc tcacaaacat
    18601 tgcgacgagt aaccgtagga gcaataagag gcggtaaaaa cccgtctctt atttttcgtt
    18661 ttctttcact gtttcccgtt caactagtac aggttcatag agaatatttg gctgtgcctg
    18721 tcctttttca atcgttgtga cgatccaccg tgcggcatct tgtcccaact tttcttttgg
    18781 atgcgtcaat gttgtcaatt ttttattccc taaatggctt aaagaagaat cgtctgttcc
    18841 tacgatcgat aggtcatctg gaatatgata gccattttct gtcaacttat ctagcattaa
    18901 actagctatt tgatcgttat agcaaatgat gccatctaaa gcttgttctt ctttcaaccg
    18961 ttgtactgtt ttttcagcga ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc
    19021 cgtcaaaaaa cgcacattat actgttcaca cgcgctgata aaacctttca tcctgtattt
    19081 tccttgaaga tcgtcgattt ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa
    19141 atattctgct gccataaaac cgactttcac atcatctaaa caaatataag aggcatttaa
    19201 ctcttcataa gcagcattga tcatcaccat cggaatgcct tgttctctca aagcaacata
    19261 aagtgctaag tttgggttat actgattact tttcgtaggc tcaacgatca aaccatctac
    19321 cccataagca atcattttct tcaaacactc tttctccaat tcgtgatcgt tattcgtact
    19381 ggctaaaagc aatgaatacc cagattctcg taattcattt tcgattcctc ggataatcga
    19441 cggaaaaata taatcagaga gatatgtggt gatgacaccg atcgtttttg ttctgttcaa
    19501 tggtgttttt tctttcttat agctgtcatc tacatatgtg cccgaccctt tttctttacg
    19561 cagatagccc tcatttacca acacagcgat tgcttgccga atcgtatgtc tgctgacgtc
    19621 atatatttct tgcagcttga attctgaggg aatggttgta tttacttcgt attcctcacg
    19681 aaggatcttt tgttttatct cttctgcaat atcttgatat tttgccattt gccttgcccc
    19741 tttttatttg tacgtacata tatcataacg agtctgcaag gtttattcaa gcaaaaatca
    19801 aggtttcttg tgctcataag gcatacggaa caggttcacc aaattctgga aatccttgct
    19861 cattccacgt aaagcattgc gcgttcgcgt gccgattagg attgtctaac ggatcccctt
    19921 tttggttctt ctctggtcga gcatgataga tcaaaatatc aaagcacccg tcttctgaaa
    19981 ccgtaaacga gttgtgacca ggaccaaatt gcttattttt ttcagaagaa acgaatactg
    20041 gctccgataa tttatgccat gattctttta ataaaagatt cttgtgctca tcagcccaca
    20101 gcaggcccat ggcatagttt tcatctgttg cactaccaga atacgtaatg aatattttcc
    20161 cattgcgagt aaggactgct ggaccttcat ttactgaaaa accccgtttt tcccattcat
    20221 actcaggtat agaaaggagg atctgcttcc cctttaacgt ccaaggatta tccatttcgg
    20281 atagataaag attagaattc ccagtaattt caggatcttg ttgggcccac acataatata
    20341 gctttccttg agaagagaaa gttgttccgt ctaaactgaa agtttccctt ttcgttttta
    20401 tttgtccctt ttctatccaa tctgtattca ttggatctat atgctgattt tcaagtacga
    20461 acatgcggtg atgatgagat tcatcacgta tagttttatg attgcttgca gcaaagtaaa
    20521 tataccattt tccattgata taatggactt caggtgccca gatcaattca ctcatcggtc
    20581 cttcttcatg agcctgccat acaatcaggc ttgcagcatg ttctaattca ttcagtgact
    20641 ttgcccttct taattcaatc acttggtagc ctggaaccga tcctgtaaaa taataataac
    20701 catctgtatg tttatatacc caaggatctg cccgttgccg aacgatagga ttattataaa
    20761 gtgtcttcat cataacaaat ttcctcacat tttctttatt ctaattacat tccaagagta
    20821 tttttctatc cctacattta attcgtcatg tttttctatt gaaaaatagt ttatttcagg
    20881 ttttattttt tctggttctt cacgtgtatt tttgtccaac agatcgctac tggttaatgt
    20941 gtagtgctct ccttgatcta gcaaattgaa atcatgttca atcgttatct tttgttgttc
    21001 atttgtgcga ttgatcaaga aaatagtcag ctgattgccg ttatctacga tcacactgtc
    21061 cacataagga acatttttta tcttttcaca atcataaaca gggcaaatga tttgttgttt
    21121 caatacttga cctttagcat atttcgataa gtgttgcatc acataataag ttggattcct
    21181 ccatgcttgt cctttagcat ctgtcagtat taaaggtatt gtgtttacta acaatgattg
    21241 acaactaatc tttacgcgat ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa
    21301 taatgtatct tccatagtaa agtaacacca atcgatggga ctaccgactt caaaatccct
    21361 atgtcttttt tccggttggg catgtacatt ccattcatca aacgctagat acattgtttt
    21421 ctcacttctc aaatatcctt ttacataatc acaagtagca acgatttctt caatttgacg
    21481 atcgaatcga taagcttttg caaggtatgt cgctgtatca tcacgctcat ttttaggagg
    21541 tttagtcaag tcttcttcat catacttatc aatatagtta tgcaaagcta aataatctac
    21601 attctcataa gcttcttcta atgcgatacg atcccattca gggtacgatg gtaaacgtgg
    21661 cgtagagctt ccaactaaga ttgtttcaat tgaatcatcc actaacttca tgacttttga
    21721 ggcttcattt gctaacctac catattcata agcactctta gcccccactt gccatggacc
    21781 gtctagctca ttccctaaac accataattt aacgccaaaa ggcttttttt gcccattttt
    21841 tattcttaaa tcactccaga aggttccttt tggaaaatta caatattcta ctaaattttt
    21901 tgcttcatca attccttttg ttcctaaatt caccgtgaat atcggtttcg cttgtacatg
    21961 actgatccat ttaaaaaatt cgtggagtcc aaaagaattt ggttctatac ctcgccatgc
    22021 taaatcaatt ttttttggtc gttgttcttt cctacctaca gtatccaccc aatcataacc
    22081 agatgtaaag tttcctccag gatagcgaat gacacctaat tctaattcct ttacataaga
    22141 gaggacatct tgtctaaacc cgtcaccatc agctgtttca tggcccggtt cataaatccc
    22201 tgtatagacg acacttccca tatgctcaac aaatgagcca aacagccgag aactgatctc
    22261 tcctaccgta aaatcctctc tgatttttac tttcacttct tcgtccaaca gctttcccta
    22321 cctttcattt cctatagtca agataataga tacaagtttc catacgccac cagcaaagat
    22381 aaaaataaaa ggagggaaaa gaaatatgag cagtacaaat cctaataaca atacgcaaca
    22441 aagaatattt ctcaaaaaat caaaaaatac tttccatgca ctttgttcaa aaatttgtaa
    22501 aaatgtgata tctttgtttt cagaaatcac atatatccag gataaaagaa acatggaata
    22561 aataagtaaa actacatatc cactaattga aagtacgatt gtctgttctt ctaataattg
    22621 attaacaaga aaataaataa tgatcgttaa cagactaagg aacaagttct taccaaaatg
    22681 ttgctttatt ccttgaaaga aaaaataaat gatatttcct gatccctcac ctttccattg
    22741 cgccattaca taaaaaacag cacatgtagc aggcacgata gtaataacag gcaaacacac
    22801 gattacccag agaataccta ataagatgta atcagaaacc acaatcaact gtccataaaa
    22861 tttagtctca actattttca tcttcctcac tctttcattc cagacatagc tgttccttga
    22921 acaaaatatt tctgtgcaaa aatataaacc actagtaatg gcagcattga aataaccgtt
    22981 ccagccatta caggtccata aaaagaaatg ttctgccctg taaataaagc taaccctgct
    23041 ggtaaagttc tcatttttga actacttgtc aatatcaatg gataaagcaa gtcattccaa
    23101 ctattcatca gcgtgaaaat ccctaatgat aaaagagtag gtttgctcaa tggcaacatg
    23161 attttcaaaa aaatattaaa ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa
    23221 tctggtaaag taataaaaaa ggaacgcatc atgaagatac caaatgcagt tgtcatacga
    23281 ggaatgatca aacctgcata agtatcaagc atgccaagca aattgacttc aataaataga
    23341 ggaatcatga agacttggaa tgggatcatc attgtcagca aaacgaaata gaacaaaact
    23401 gattttcctt tgaaacgcat acgagcaaat gcataccccg caagagaatc aaagaataca
    23461 gaagtgatca ctgtccctcc agcaaaaatc actgtatttt tgatgtaatc taataaaggg
    23521 atcgttgtcc aaaccttaat atagttatct agggtatagc tatctgcaaa tattctaggc
    23581 ggaaatgaga taatgtcttt ttcgtgttta aaagaagaaa taatcagcca caaaaaagga
    23641 aacgcaataa ccaacaatat aactagcata ctaatcgttc gaatgatttt tttaccacta
    23701 actgcttttc gactattcac agtaactgta atcgagcttt ctggtgccat acaggtttcc
    23761 ctcctatttc atttgttttt ctttccgtga catgtacaca ttagtggaga tagctatagt
    23821 tgcaacaatg ataaacaaaa ccgaagacag tgctgatgca tagcccaatt cataaggtgc
    23881 tgtaaatcct cgactgtaaa tatattgaac agctgtttct gtcttaaatt gtgggccacc
    23941 agcggttgca acatatactt ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat
    24001 acaaaaacct aaggagggaa cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt
    24061 tgttgcacca tccatttgag cagattcata caaagaggga gagattccct gaatagcagt
    24121 caagagaatc gtcatagtaa caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg
    24181 catagcaagg gttggatcgc gaaaaaattg agggatcggc aagccgactt gtgaaaacaa
    24241 gtaaggaatc gcccctacat tcggatcaag caacatagaa aaaataatcg caattgacgt
    24301 caatgaacaa ataactggaa tgtaaaatgt cgaacgacaa aaacgattga atctggtatt
    24361 tttagataat gctactgctg ctaataaacc aacgataatt tgtgttggcg tttcgagtac
    24421 ggtgaaatat aaagaatgaa tcaacgaatt aactgctcgt tcatcactaa atatacgtgt
    24481 gaaattttca aaacttacaa aatttcttga tgtaaagaaa atattcaaat ctgtaaaact
    24541 aatagcaaat gttcctaaaa gcggtatgac agtaaataat aataaaacta aaaaagatgg
    24601 agttaaaaac aaatacgcag cacgatcagg acgattccaa tatttggtga atccgttcat
    24661 atagtccacc tcatctttca ttttgataaa atatacaatc ttggtttaaa cgaatctgaa
    24721 taagatatcc gaacaatcgc tgtgattttt gcaataacac ctatctgcaa atactaggtc
    24781 gatcattcag atatctgtat cttgccaaca gtctatcctg tataaagttc tgtactattt
    24841 ttgcagtaat tgattgatgg catcatctgc atcgttcatg agtgtctcag gatcttctcc
    24901 tgctaataat ttttcaatca gtggattgat gatatcgtta ttgatcgcag agatttgggt
    24961 aaaacctggt aaatatggct ctgcatagtc catttgtttc gataattcag atacaatagg
    25021 atcatttttt acttcttcat cttttgctac tgaatccaag taggcaggaa aaccattttc
    25081 gatactccat tttttaccaa tttcagttgt attccaatat ttcaaaaact catagacagc
    25141 ttctttttta ttattatctg tacttgccgg aatggcatat cctacgccat ctaaaatagc
    25201 tgtttttttg tctgaatcac tcccttgtgg taaggtagtc acattataat caatctcgtt
    25261 tgctttcaat ccgttattta accatggccc attgatttca atggctagtt gaccagcatt
    25321 cattaaattg tccatttcgg cgccagaaat cgattctgga ccagtcttgt ctacatggat
    25381 cattttttgg atcgctttta acgttttaag gttctcagga gacgcaaatt cagacttagt
    25441 ataatcatcg ttcactaatt taccgccatt tgctaacatc cagttatata acggtgcagt
    25501 tccatcctta ttgaagacaa aacctgatac atttttacta ctatcagtta attttggtgc
    25561 catttctgtc aattcttccc aagtagtagg tggttgttca gtatctaatc cagcttgttt
    25621 aaacaaatca ttattccaat acatatacat gccttgtacc tgcatgggga caaaatactg
    25681 tttaccttct acttcaccta attcaatagc cgtttcttta aaatcagatt tatctactcc
    25741 atcatattcc cagaaatcgt ctaatggttg taccgctccg ttttctacat actgtgcaaa
    25801 atctggataa tttaatgcaa taaagtcagg tgctttttta gaagaaatcg ctgtaggaag
    25861 tttttcattc aaattggccc aagtcatgat atccatttca attttgatgt ttttatcatt
    25921 tgttttatta aattcattaa cgatttcttg caatatttca ccatcagatg cagtaaaccc
    25981 attccaaaaa gtcaaagtca cttctttatc agaatcagat tctgctgaac ttccgccacc
    26041 gcaagctcct aaaatacctg ttgcagctac caacaaagag ccaattaacc attttttacc
    26101 tttcattttt tgttcctcct tcatctgata aacgacatct gaccacattc caagatagcg
    26161 gtttcaataa acaattgatt ttcccatctt cagcttttac atttgttgaa tgacttggtt
    26221 ttacttgttc acttttagga agattagttg cttttgtatc aaatccactc atttcagaaa
    26281 aatcaatcac tccctctaat gataattcag acaagtgaac agttagattc atttcttctt
    26341 ctccacggtt aactgcaaat atgacaagtt ctttgtcatt tattacggct atactttcta
    26401 aatacggcac ttcttcaaaa tcttccgtcg catagtttgc tgaacgaatt tgtgggctta
    26461 acacctctcc ttgtccatac acagcagctt gcataaatgg ataatagatc gtctgtctcc
    26521 aagcttctcc gtttgtttct gtcctgattg gtgcaataac gtttaccaat tgagcaagac
    26581 aagcaatctt tactctatct gcatgtttca ataaagtgat taatagacag cctaatagta
    26641 atgcatcttc aaaattatag ttatcttcta gtaaaggagg agcaatctgc caaggcgctg
    26701 cttgctcatc ttgttccgct gaatgatacc aaatattcca ttcatcaaag gacaggttga
    26761 tctgcttctt actatgtttc ttcgccttga ctgtatcgca catcgctgtc acttcagaaa
    26821 tgaaccgatc catgtctaag gaacgtgcaa gataatgaga aagatcattt ttttggttgc
    26881 cataatattg atgcagagac aaataatcta tttgatcgta cgcttgatca agaactgtta
    26941 actcccaatc accaaatgta ggcatttgcc gattcgaact tccgcataaa accacctcaa
    27001 ttgaatcatc tacgagtttc atcgccttag ccgtttcctc tgctaaccgt ccatattctt
    27061 ccgctgtttt atgtccaatc tgccaaggac cgtccatttc attccctaaa caccatgtct
    27121 taattccaaa agggctctct tgaccattcg aacgccttaa atcactccaa taagttcctt
    27181 taggaaaatt acaatattcg actaaattac gagctgcatc tactccacga gtacccagat
    27241 tgacagccat attgatttcg gcccccacat gctgacacca gttataaaat tcatgaatcc
    27301 caacttcatt cgtttcaatt gtccgccaag ctaaatccaa ccttctaggt ctatttgcta
    27361 tgggcccaat actgtcttcc caacgaaatc ctgacacaaa atttccacca ggatatctaa
    27421 tcatgggaat gttcaattct tttaccaagt gaatgacgtc ttggcgaaat ccacttttat
    27481 tagctgatgg atgatctggc tgataaatcc cttcatatac tgctcgtccc atatgttcaa
    27541 taaatgaact gtacaaccgc ggatcaactt tgcctacacg cttgacacta ctaatcaaca
    27601 ttgtggcttc cataactgac ctcctttaat ttatacatga ataatacacc tcgttgaaag
    27661 cgttgtcaat aatataaatg ttaaacttac ttatttttta tcgattttaa ttaaccaaaa
    27721 tattaataat gattaaaaca aata
//
LOCUS       DAZKAS010000023        27733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000023 DAZKAS010000000
VERSION     DAZKAS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.023"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..1246)
                     /locus_tag="ACG8H7_000849"
     CDS             complement(167..1246)
                     /locus_tag="ACG8H7_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8982964.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            1354..2757
                     /locus_tag="ACG8H7_000850"
     CDS             1354..2757
                     /locus_tag="ACG8H7_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8982965.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            3106..3738
                     /locus_tag="ACG8H7_000851"
     CDS             3106..3738
                     /locus_tag="ACG8H7_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFP8982966.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4326..5765
                     /locus_tag="ACG8H7_000852"
     CDS             4326..5765
                     /locus_tag="ACG8H7_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8982967.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            6060..6278
                     /locus_tag="ACG8H7_000853"
                     /pseudo
     CDS             6060..6278
                     /locus_tag="ACG8H7_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            6527..6742
                     /locus_tag="ACG8H7_000854"
     CDS             6527..6742
                     /locus_tag="ACG8H7_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982968.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6959..7215)
                     /locus_tag="ACG8H7_000855"
                     /pseudo
     CDS             complement(6959..7215)
                     /locus_tag="ACG8H7_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7402..8313)
                     /locus_tag="ACG8H7_000856"
     CDS             complement(7402..8313)
                     /locus_tag="ACG8H7_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8982969.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8332..9336)
                     /locus_tag="ACG8H7_000857"
     CDS             complement(8332..9336)
                     /locus_tag="ACG8H7_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP8982970.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9333..11456)
                     /locus_tag="ACG8H7_000858"
     CDS             complement(9333..11456)
                     /locus_tag="ACG8H7_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP8982971.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11461..13908)
                     /locus_tag="ACG8H7_000859"
     CDS             complement(11461..13908)
                     /locus_tag="ACG8H7_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8982972.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13895..14284)
                     /locus_tag="ACG8H7_000860"
     CDS             complement(13895..14284)
                     /locus_tag="ACG8H7_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8982973.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14344..14847)
                     /locus_tag="ACG8H7_000861"
     CDS             complement(14344..14847)
                     /locus_tag="ACG8H7_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP8982974.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14860..15084)
                     /locus_tag="ACG8H7_000862"
     CDS             complement(14860..15084)
                     /locus_tag="ACG8H7_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982975.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(15188..17098)
                     /gene="ltrA"
                     /locus_tag="ACG8H7_000863"
     CDS             complement(15188..17098)
                     /gene="ltrA"
                     /locus_tag="ACG8H7_000863"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP8982976.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(17844..17981)
                     /locus_tag="ACG8H7_000864"
     CDS             complement(17844..17981)
                     /locus_tag="ACG8H7_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP8982977.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(17978..19162)
                     /gene="mobT"
                     /locus_tag="ACG8H7_000865"
     CDS             complement(17978..19162)
                     /gene="mobT"
                     /locus_tag="ACG8H7_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP8982978.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(19421..19711)
                     /locus_tag="ACG8H7_000866"
     CDS             complement(19421..19711)
                     /locus_tag="ACG8H7_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982979.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(19701..20843)
                     /gene="dcm"
                     /locus_tag="ACG8H7_000867"
                     /pseudo
     CDS             complement(19701..20843)
                     /gene="dcm"
                     /locus_tag="ACG8H7_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(20919..21083)
                     /locus_tag="ACG8H7_000868"
     CDS             complement(20919..21083)
                     /locus_tag="ACG8H7_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8982980.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(21076..21750)
                     /locus_tag="ACG8H7_000869"
     CDS             complement(21076..21750)
                     /locus_tag="ACG8H7_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP8982981.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(21828..23168)
                     /locus_tag="ACG8H7_000870"
     CDS             complement(21828..23168)
                     /locus_tag="ACG8H7_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP8982982.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(23251..23586)
                     /locus_tag="ACG8H7_000871"
     CDS             complement(23251..23586)
                     /locus_tag="ACG8H7_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFP8982983.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(23596..23970)
                     /locus_tag="ACG8H7_000872"
     CDS             complement(23596..23970)
                     /locus_tag="ACG8H7_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP8982984.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(23983..24297)
                     /locus_tag="ACG8H7_000873"
     CDS             complement(23983..24297)
                     /locus_tag="ACG8H7_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP8982985.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(24410..27637)
                     /gene="fss3"
                     /locus_tag="ACG8H7_000874"
     CDS             complement(24410..27637)
                     /gene="fss3"
                     /locus_tag="ACG8H7_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFP8982986.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7922 a         5833 c         4924 g         9054 t
ORIGIN      
        1 acaggtaact catttgtttg ataccgtaac tgcctcgtta ttctaaatca gtgaggcctt
       61 gataatgaac tgaggtatga tagtcttcag acgattctgg ccccaggcca gctttccaga
      121 catgaagcca tgtcccgagt gaggatcgtt atcaaggaat aatgggtcaa gccaccaacc
      181 gttcttgctt gaatgcctgg acgaatgtat catgtggtgt cgcataaccc aaaattttcc
      241 gcgggtaatc gttcatccaa cgctggatcc gcaaaatctg gttctcgctc acctcactaa
      301 tcgggcttcc cttggggacg aaacggcgaa ttaatttgtg ctggttttcg ctcgtcccac
      361 gctcccaaga agcatagggg tggctgaaat agacgtcagc tacatcttgc agagcatcgt
      421 gtagcccaga aaactccgag ccattgtcgg atgtaacggt cttaaataat tgatcgaagt
      481 tagcgccagc ccgttctctt agctgttgaa ccgcttggtc caccgattcg gtgtccttcc
      541 cgttcaactt taggatgact tcaaagcgtg tctgccgttc agccagcgtc agcagcacgg
      601 catccgactt ggtcttgttt cccactaccg tgtcgatttc ccaatgaccg aacgtttccc
      661 gtgtttcaac ttctttaggg cgttcttcaa ttgacgtccc gagaacacgc ttgttgggac
      721 ggtgtcctgg gcggaccgct ttgattttac gggacagttt ttcaagcaag tccatgttct
      781 ttgttcgcat gattccgcgg tcaatccatt gataaagagc ggttgtgcca ggaatgatgg
      841 cacaatcgaa cagctcgtgt tttctggcaa atccgaccac gacgtccggc gaccattttt
      901 catttaacat tttgtcatct gcccaatcaa tgaaggcatt tgtgttcgcc catttggggc
      961 gtctgccaca gttcagtcgt tgcttttcat agaaggcctg tcctgcatca gcgtcataaa
     1021 cagaatagta ggtgtcatac acttttccat tttgcgtttg gcgttttaac tgggtgatgg
     1081 ttccgcgatc aatctcattg tttatggttt gcggaacacg tcccagcact ttggcaacct
     1141 gacggttgga atagccttcc tttttcagga tagcgatttg gcaacgttct gagtaggaaa
     1201 gatgttttcc cttgcgtgaa actgtgttat catgggtttg cgtcatgtga attcatcctt
     1261 gtctattgag agttgtggta acttcaatat aacatggaat tcacgtggcg ttttttttat
     1321 tttaaatggc taagttcatt ataaaatccg ccaatgaaaa aatctttaaa acttgcatca
     1381 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     1441 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     1501 gtaacagcag agagtagtga acttttgaca acttcatcaa gtgaaagtag tacgacttca
     1561 aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
     1621 tcaacaactt cgtcaagtga aagtagtacg atttcaagta ccgaatcttc aacaacttca
     1681 agtagtacaa gtgaaataac ttcttcgaaa gtgacaaaac caaaccatat agaatcttca
     1741 aaaaaggaag cgccaaaaac ggaaggacaa acaaaaactt tttcgacacc acagcaaata
     1801 gaatcaacag aatctttcca atcacaacca atactatctg gtaatgaaga aattcatttt
     1861 gacaaaaatg aatctgtaga aaattttatt atgaaaattg gtgagtctgc gagaaaaatt
     1921 ggtcagaaat atgatttata tgcttctgtt atgattgcac aagcgatctt agaatctgcc
     1981 agtggtcaaa gccagttagc tcaggcaccg aattacaatt tatttgggat aaaaggaact
     2041 tataatggaa actttgtcat tatggtaaca aacgaagatt tgggtaatgg aacgctttat
     2101 acgacccaat caaaatttag agtgtatgaa aactatgagg aaagctttga agattatgcc
     2161 aaattattaa ctaagggaat tagtggaaat aaagattttt atgcaggagc attaaaagct
     2221 aatagtaaaa catatcgaga agcaactaaa ttccttacag gacggtatgc aacagataca
     2281 caatactatt tgaagctaaa tgaattaatt aaaacctacg atttaaccaa ctatgataaa
     2341 gaagttaaag gtccacaaat gagtaagact ggataccaag tgcctattca taattattca
     2401 ataaccagtt tgtttggaaa tcgtggaaat gaatttcacc gaggacttga tatggcagct
     2461 acacagggtg agccgattca tgcagttaaa gctggtaagg ttgttaaagc agagtttcac
     2521 tactcttggg gaaattacgt ggtgatacaa cacgaagatg gatcaaccgc gttatatgct
     2581 caccagcaac aatacctagt aaaaactgga gataatgttt tgcaaggaca aataattggc
     2641 tatgtaggaa gtactgggaa tagtactggc ccacaccttc atttggaaat ttgcaaagat
     2701 agtagtcttt ctcagagcat gttagttgat ccgaaaacct tattattcgg agactaaaaa
     2761 aggtaatcaa tctgacaaag taacaagaaa atggttggat tcttagacct ttgatcagga
     2821 tattgagact ggaattgata tctcgattac gatgggtttg acaaataaaa caagtccatt
     2881 atcgaatatc gagtgtatta ttgccgtctt tatgctcata ttttgagcag gtttcactag
     2941 atgaaaacaa tttatctatc ttgaggattt cgtgtacgta tcgttcagtc ttgtattgta
     3001 aattagtcat aaaatcagac taagaaaaat tggaaatatt tttttctgat ttattaaaag
     3061 ttaattgaaa ttatatggta cttctttctt gggagggtag gtgcagtgaa ggaaagatat
     3121 ggtcacctta ctgttgttga ggagattact cataatggat ataaaaaata tcgttgccta
     3181 tgtgactgtg ggaatgaaac gattgtatat cgatcaaact tactatcagg tagaaccaaa
     3241 agttgtggat gtggaaatta taaaaatagg cataaatata aaaatttgac aaataaaact
     3301 tttggtaagt tagttgcaat caagccaact aaactgcgaa gtgaaggaac tgttgtttgg
     3361 gaatgtcaat gtctgtgtgg gagaaaaaag ttagtatcgg caaagcgact tctaaaaggt
     3421 gatgtaaaaa gctgtggatg tttgaaatca aaaaagcatc acattgaaaa taaaaaattc
     3481 ggattactta ctgcaaagta tcctttggac aataactgtt catggaaaac aaaatgggtt
     3541 tgtcattgtg agtgtggcag tatgtgtgag ataagttata gtaacttatt ttttggacat
     3601 actcaaagtt gtggatgttt aaaaaggcaa gaaacaagga catgtgttga agggacagta
     3661 atagagtgtc taacgtcaaa attaccgaaa aataatacaa gtggtgttaa gggagtttat
     3721 cgttctaaag agaaatagat tgcgtatata actttaaaaa aaaagaggta ctatttagga
     3781 tcgtatgatt ctattaagag tgcagctcaa gctagaaaag atgctgaaaa taaattgttt
     3841 caacctttga ttgacaaata tcgaggtaat cattaattat tgtggataat aattaacagt
     3901 gatatataaa catctataat atattattca tactattgat atttagtata atcctatgga
     3961 attgaatatc atagcaatgt atttgaaaag caaattggaa aacaaatagg tgcaacacca
     4021 taaatatgaa aaaattggga aaaagtagaa tggaaatttg cttacgtctc acgagatagc
     4081 tcacactatt aatctgaaga acaatgaagt cgacttttag aaattgctag aagaaagtaa
     4141 agcgaattat cactagttac tatagagtaa gttcaaataa agcatctcca acttacatgg
     4201 ttacatgagg atatcaaatc gaaattactc aagcttccta tggctagagt cgcgagaagt
     4261 tgttgggcag tagacatagg ttatattgtt cttagctaca cgatcattag aaaggaaatc
     4321 ctattatgta tagttatcaa gaagatgcaa aagtccaaaa aaatcgctgg tggattttga
     4381 ttgctatatc tatgtttacc ttcatgtcca cattggatgg cagtattgtc aatattgcac
     4441 tacctaccat ctcagatgat atgggcatac caatgaatca agcggaatgg gtagtgtccg
     4501 tttatctgat gattgtctgt tcttgtttgt tgctgtttgg taaaattggc gatagccgtg
     4561 ggaaaatcaa agtatttcgg attggtacgc tgatctttgt cttgggatcg ctgttgtgtg
     4621 ggttcaatca ttcgctaggt attttattgt tcgcacgggt ggtacaagcc attggtgcta
     4681 gtatgactat ggctacaaat gccggaatca tcacggaagt ctttcccata catgaacgtg
     4741 gtcgggcatt gggagcaata ggtgcgtttg tttcattagg ctcgattgct ggaccaggtc
     4801 ttggcggctt gattttgtcc caattttctt gggcgtacat tttttggatc aatgtaccgg
     4861 tgggaatcgt aacgatgttg attgctgaaa aattcttacc aaaagatatc accatgagcg
     4921 gccatacaat cgatggaggg ggctttgctg ccattgcggc tacgattatc agcttttttg
     4981 gagcaatctt tttggggcaa gaaagtggct ttttccatcc agtccccctt gtactgttcg
     5041 taatttcagt gattgccttt gtcagctttt taaaagttga aaagaaagca gcctctccat
     5101 tgatttcttt cggtattttt aagaacaaga tttttaccat gagcttgatt acagccttgt
     5161 tgattttttc ttccaacttc tttgtcaatg tagtgattcc tttttatctg caaaatgctc
     5221 gcgggttgag tcctagctat gctgggctgt tgatgatggt ctttcctttg atgatggtcg
     5281 tcggggcgcc tttaagcggc tacctgaccg accgggttgg cccggagatt ttggtattag
     5341 taggcttggg cttattgtcg gtaacccagg tcatgtatat gtttttgtca gtggactctc
     5401 ctttgtggct atacattgtc gctactgcca tcatgggtgc cggcaatgcc ttgttccagt
     5461 cccccaataa tacgatggtt atgagctcgg tcactatcga aaacttggga ctggcaggct
     5521 cgatgaattc ctttgcccga aatttgggga tggtcagcgg gatttcgctt tctacgacgg
     5581 tcttatatca agcgatgtct gctaagatgg gccagcaagt aactaactat atcgcaaatc
     5641 ggccggatgt ctttttatat ggaatgaaga ttaccttttt aggcagcttt gttttgtgtt
     5701 ttgttgcctt tttcctgact atctggcgta tcaaaaaaca aggaggtatt cagaaaaatg
     5761 tctgaaaaaa ccactgttga agaatttttg gaggcctgcg tatgggtgag tgggatttcc
     5821 atggatgatg gaagatgaag tgaacttcta aaagccgaaa aactcaagta ttatcttcaa
     5881 aatactagtg ctcaatgaga tgcttgattt gatcacaaga agactgcgaa agccgctggc
     5941 aaccgcaagc taggtatccc accgatggac tacgttttag aggctactgg ttatgaatac
     6001 ggagccaacg ctctcatctg cataagaata catcacccag attatgtaaa gattttcgat
     6061 acttctgtca aaaaatcatt aagatagata aatgatttcc gtctagtcaa attggttcag
     6121 aatgtagaca cacagatggt aagaagtcac tcacaattcg agaatggact tgtcctattt
     6181 gccgtataca tcataatcga aacgtcaacg atagtatcaa tattttgacc aagggctatg
     6241 aatacaagca ttgccttaga aaacatcaag aaccttagga acaacgggga tagcttggtt
     6301 aataagagat accgctgttt aggcttgcaa gtatactctt ttatcaagaa ttccgtggct
     6361 ttagtcatga gaggcttaat actatgaaaa ttttctttag catttttgat ttaggttcct
     6421 tttctaatta taataagatg accactacag taacctactg aaatatataa acagccactg
     6481 tagtaccatt ttcaaagtag tctatgagag gagaatcttc attcttatga acgctggaat
     6541 tgcggatatg aatttaataa aaaaaaccct taatgatttt acttccaatt caatatcaaa
     6601 aggtactggt atcaatttaa gcacaataaa aaagctgaaa agtggtgaaa gatcagtgga
     6661 aaagctgaat cttttagatg ctataaaaat aacggaattt gctatgaaaa atggaaaagc
     6721 tgagattgaa atatggagat aattatcgtc tagtgaatta cttgccactt agctgaattt
     6781 aacgatcctt ggtgcttgaa gggggagatt cttgggaata gagcatactt gcaagcctat
     6841 gtctttacta acaacgatat ctcttattaa ccaagctatc cccgtagttc taaggctttt
     6901 acttttatct aagcaataat cgtattcaaa ggaaattttt gacccttaga aagtctcatt
     6961 ttatcagacg tgagcttagg cattgctgaa ttttgttaat tcggttttag gttgtgcttt
     7021 gagcataata tgaaagtatt ctttatcacg atttcattca tctaaggtaa tgtgatgtga
     7081 ttctgaaatt ctttcaaagg cagtttttgc ataagctgac attacatcat caatgacatg
     7141 tttacaatgt ttcactacta aaataagggg gtaaccaaga agaaaaactg aatgattatt
     7201 tgtatctaat tccatggtat atcaaccatt tcctatatag actgattatg acatggatta
     7261 aaataaaaag gcactatgtc ttaattgtcg gttttcgagt taccgaatgg cattcatcac
     7321 ccacctatag aggatgggcg acttctgcct gaaataagtt acactactta taacttctaa
     7381 taaaattaga ttgagtcatt attatttaac atcaacgatt ttccagtttc catttttttg
     7441 taatactaat ttgtactgaa agtagctagt ggtttttgat acattatcaa ggtattttat
     7501 ctttgtactc actagaaaat tttttccgtt ttgtagataa actggattct ctaactccga
     7561 aaactttaag tcaatattga ttggtcgcat cgcaccattt tcaacataat actccaattc
     7621 tttttcagaa gctgatggat aaagtttaaa aaatgtttct aaaaaatcgg tgatttcttc
     7681 cgttgttttg gcatcaattt ctgaatcgtt ttctacttgt tttgcttcat attttgcctt
     7741 gtctggcttt ccaatcattg ttggcaaact agttactaaa tgattcctgt tttcatcttc
     7801 aaagattgtc actcgatatg cagaagaaat tgattgcgtc ttttttccct ctgtaatatg
     7861 ttgctccact aaaaatgaaa cgacaaattc ttccgagtgt ttttcaacat ctagaatttt
     7921 tacagattga acttcggaac tcgtgggaat atctgctcga accatatctt ggattaaagc
     7981 gagaccgttt tctgtaaggt actgttcgag attactcatt ctcttttcaa ttgcttcttt
     8041 gttatttttc catgaaaaat attctttcgc aaaatcctct gtaaaacttt caacgccaga
     8101 agtattcact accttctctt ttataaccac tttttcatga gttgtatgtt gatcaatggc
     8161 ggtgaaattt ttataaacgc caaaagcaag actacaagac aataaaagcc aaagggcaag
     8221 caccattttt cgatgcttac ccacggatac aactcgttct tttttctttt ttgggctctt
     8281 ttccttttga ttccgttcaa tttttatctt cattcttttt cctcttttct cttatttgta
     8341 tcggccagct ccgataagat gctgccgcca atagttttcc gttagattcg tccaccccac
     8401 tggatctcct gcatgaaaca tgcgattctc ccctgcgtaa atgcccacat gggtcacata
     8461 agtaccggca tcataggttg aatgaaagaa aactaaatca cctggtttgg catcttttag
     8521 atcaatatga gtcatggcat catattgatc ctgagccacc cgaggaagtt tcaggtcaac
     8581 tttggcataa caccattggg tcaagccact acaatcaaac cccgtttctg gacttgagcc
     8641 accaaacaca taggccgttc cttcatactt aaatgcctca tcaaaaattt cttggactat
     8701 tttgtcatca aactgagttg tagttaggta ttgcttcact aataaacagt aaaacatatt
     8761 gccataatta taccgccacc caccattttc cttaatcgcg attgggttgg ggtaatcgac
     8821 tttttgacca cctgattttt cctttgaaaa agattcagct aaagcaaaac tatgcttctt
     8881 cccgtttttc ccgacatgat ctagataccc gccaccatag ttataggcct gaataatact
     8941 atcttgatca caccctagtt ctttggaacg cttaaccaaa gacgcaaaat agagacagcc
     9001 ttgtttgatg gatgcctcgg tacttaatga attaggtggt aatccggctg actcagaact
     9061 ttgcataacg tcttccattg tgccaccaga ttccacttga ataatcgcca aaagaattgg
     9121 aatatactct gttatatccg attctttggc atacttttcc accattgcac ggtgtttcaa
     9181 tacttcttct gagacactag ttccgccagg agaagtgcca gaactaccat ttccctcact
     9241 gtcttcacca aacaacaaac ccacacacat caaaataccc ataaaagaga tggtcagaaa
     9301 agttaatgaa ataacaccca ctttcttcaa gttcattttt tctcacctga ccttgatttt
     9361 ggacgatggt tcattctttt ttgtactttt tgattggtct gctttggcag agtggacctc
     9421 tgtttcactt gttggaaaga atttttctta cttgctgatt ggcttttccc ttttgaaatg
     9481 atttcttgac tagttagttt tcgtttgatt tcgggattca gatttgctct cacaatccgc
     9541 tttggtgctt cttttttcag catcatatct ttctgaggtt gagctgcttt atctattgga
     9601 atattcgccc ttctttttcc ctgacgattt gttgcttcac tcagtatctt gcgtttttcg
     9661 tccattttcg catttcttcg ttgtcgttgt ttttctcgca aatccactcg atccgctttt
     9721 tcttggaaca gtccgcgctt gaattcttct ggtgcttttt tcgacttttc gattccttta
     9781 tggagattgt atttcaaatt cgttggcgta ttccttacct gatcctttac ttgtttagtc
     9841 ttatctttca ccaaagcttg ggtatccaaa actttgccga tttttctccc agccaaattc
     9901 attcgggatt gtttcttatt atttgaaact ggattctcct tagttaagtc agatagtatt
     9961 tcgtgatcgt tcggtaatcg tgaattcttt cgtagagaag aaccggatgg cttaaatttg
    10021 gattttgatt gttcctttgc caacttattt ccgactgctg caccaccacc aaaagccaaa
    10081 gctcgtccca catttcgatt gagttgccgc aattttcgat tcattaacat ttgcggtttt
    10141 cgcatgacgc gtcttcccat tgactggcta tcattactct ggaggttgaa catactcata
    10201 atgtcgccca atttgaaata gataccggca aaggtcacaa tttgcagaaa agcaaccatg
    10261 aaaaatggat aggttgccga aatattgaaa aacatcgtgg agatgctaaa tgcagtcgta
    10321 atcaccaaag taacacccgc tcgcatcata atcgtattga ataaacgaat aatcgccttc
    10381 tttcctaaac tttcataagt cggcaacatg gacaataaga aactaattgg cagaaacatc
    10441 gcaaagataa tgaagagaat ttgcgagaac aacataatcc ctgttagaag aaaaacaaag
    10501 aaggaaatgc caatattgaa caataccaaa aagaccacgg tccctaaacg gttcatggtc
    10561 ttggtaatac tgagattcgc attgtcattg tcttcaatct ctgccttgac cgcttcttcc
    10621 cgatctttgc ctttgttttc atctggactc acagatagaa tcttgttgac ccggtcttca
    10681 ccaatttctt ctatgttgga atcatcaaat tgcagtagta accaaggttg ttggacttga
    10741 atcgaaaaca aactatcccg aatcaaatcc acactatctt ttccttggct ttctgaattg
    10801 ggtacaacga tctcggttcc aagggtaagg gccgcttcac taacatctga gctaaagtca
    10861 ttgatttttg tgatataggt aggcgcataa gcaatgaacg aacccgatag tagaaaaatc
    10921 atcacaaaat taatcacggc tcgaactgct ttcgtggttt ctcgttttaa taatccggta
    10981 tacgccacat acacccccag agctaaaatc atcaataata gaaacccaaa ataaaatccg
    11041 gaggtctgga gtccattttc agtaatacca gccaatgttt ggatattctt tccaatactt
    11101 tcagccgtat ccgaaatgaa atctaatttg taagcctctt gaaccacata acccgtggcg
    11161 ttcgacaaat acaaactcac cgtccaaatg aaattagtaa tggcataaag accatacatc
    11221 acgcttttcc ctaagccatc accccaattc caaggcaacc aatcccaaga gctatccaca
    11281 aagaaatcta gttgatagtt gttcagtgaa tatttggaat atagatttcc tgcttcaact
    11341 gtatcatcaa ctagccctgt cgcctcggct accgtaccaa ctaaactcaa tagaactatc
    11401 agagtgagta tgctaataaa aacgattccc acaatccgaa aaagtttctt tttcatcccc
    11461 tcactccatt ctctcttgaa caggtggacg ggtatcaaag gcatggaaga gttcttcaaa
    11521 gactggatgt acctgaacca cgcctacccg accatacaaa tcctgcatca agcattggcc
    11581 attttccaaa tctcgtaggc gtttttgatt gctttcatct tctcgatcca agccaaagaa
    11641 ttccaaggtt ttcttgattt catgaatatc ggtactgcga aacgcaaact tcaacccgat
    11701 attattcttc aagcgttcat ccagtaaatc atcactattt tgcgtgacaa agtaaacccc
    11761 ggcattcata gcccgcccag cacgaacgag cttattcgat aatgttttcc cttgcgctac
    11821 ttgaagaaaa ctccacgcct catctaagtc tacaattttg aagatttctc gattactatg
    11881 gataaagtcc aaggcaaaag tggaaatgac gattagcatg gaaacactaa gcaattccat
    11941 ggtggtatat tcttcaaaag tggtttctgc atctggtaaa accaaatccg ctacttgaat
    12001 gatatttaac tgcctatcta aactaatcga ctgggtaatc gaaccatctg agaaaagcaa
    12061 atgggcaaaa tcataatcgg taaaggactc gatgtgatcc gcgatatttt ccgcaactgg
    12121 tgagccttct tttcgaagct cattaattac ttgtaataat ccccgttgat tgctttgggt
    12181 aaccgtccga atggccttac gcaacactgg aaacttctcc ccatctcgac tagaaattcc
    12241 tgttaaaaag gtcagaatat caattgctag actttcagaa tccttctttt tctccataat
    12301 cacataagga tcaagtaatc cttgattttt tggcttacta gttaagttga gaatattgat
    12361 ttcatccgca atttctggca aggtttcttt ccagccaccc cgttcccctt ttggatcgac
    12421 aatcacagct tgtccaccaa aaaggacggc gtaataaact aagagattgt tactaaagga
    12481 ctttccgcca ccgagtgaac cgaggaatgc cgctgctagc gcattggtaa ctgaatcctt
    12541 gaccccttgt gaggccaatg ccggtttcag gtaaacattg cgccccgtat ctacgttata
    12601 accaaagtaa atgccttcca actcccctaa catctgagtg gcgccaaaac caagtccggc
    12661 gagaaaatca gacgtaacat actggatata atcattcatg tatcgcttac tagaaggtaa
    12721 gaattcttca tgtagcccca tcatatcgcc aaaaggccgg actagtttta cattggtatc
    12781 atcataaaaa tctagcactt catcacaccg gcgctttagt tcatctactg attcggcact
    12841 tactcgaacc acatagctca gtttgtacat ggattctttg gactgatcca aggtagtttc
    12901 caattcatct acggaatcca atgcatctaa gacattggaa tttgtttcat tgtcagattg
    12961 ataggcatgg ttgtccaagt cttttaattc ctttttctta ttgcgaacgg tagcaagtgc
    13021 ctttttgttc gtcacaatct ccacattcat actagtgtca attgggaatg taaattgttg
    13081 ttgctggtaa tagaaaagtt ctgaggaggg aaactccatt tcccccacaa ttgtattaat
    13141 tgttaagtac gctacatatg attcatgatt ctcatgttcc atgcataagt accggggctt
    13201 ttcttcaatc aaacaacgac tgggacgtaa taagtcgtaa cgtttcacca aagtttccga
    13261 cttcagcttt ttctttggta gctgaaattc atattcttca aatggtgtac ccttttcgcc
    13321 atagatatgt tcaatcaaat acatcagatc actcggtgtg actcgtctga ctttaaatct
    13381 tcgagccaat ttactttcca ttagtttttc aagcttggta tatcggcgaa tttcttcatt
    13441 ggataaggaa acaaaatcgc ccatcaaatg atgattcact ccatagacaa attcttgaaa
    13501 gccggagaag aaagatttct tgagattctt tagattgacc tcttcatctg tcgcaatcag
    13561 tttgaagcca ataaaaaagc gataatcaat ctgactatcg ccaatcatgg atactaatgc
    13621 ctctgtctgt aaatcaattc gttgtttcgc tacttctgtt aatcgcccgg taatttcctt
    13681 ttttgatcgt tcttgcgtgg cacgaatact actttctgtg gcaatttgta aggcatgaat
    13741 cttgccttca cgattttgcg caatcaattg acgaaagtta tcgtggactt gatatttctg
    13801 ttcaggtgac aaaaaggagt aattgtaagg tattaactca tagtaggcaa aacattctcc
    13861 ctcttgatta aagacaagat tattttcaat atacttaatc gggtacatag ttcacactcc
    13921 ttaccagagt aatgggttcg tcccatcgtt tcttctgttc tttgactttc tttcctgcga
    13981 aagtgacctt aaatcgtaag tgatagctaa taaaagatcg taaaaatccc aatggtttct
    14041 taccgtcaaa cgttttttgc gacatgaacc aagtaactgc caccggtata cctaagtatt
    14101 ttaaaaaggc cccctctatc atagatagtg gaggcacatg ggcaaatacg atgacaaaca
    14161 gcaaggataa aacaaaccaa gccatctggt taaaagtcac aggaaaaggc agttgaaagt
    14221 cattaattgc ataaatgacc ttttcaactg cccaaatact ggtgtagctt tttattttct
    14281 tcatcaaatc attcctttaa aaagaaggat agtttagaaa tcaactaccc ttcaataatt
    14341 ttgttaatat ggatactcaa aaacacctcg actcgttacc aaataattgc ctccaatctc
    14401 caagtctcta ccacacgcat caaaatcaat atacgtcttc aagtgttctg gtacttctcc
    14461 aaaatcacca gcttctacaa tcaagaaata tgctacatca ctcatatcat cgcaatctgg
    14521 ataatagata atatcatcca catgctctac catctcctca aaagaattga agaaagcatg
    14581 ttgaatctca gaagcaactt caccaactgg cgttccttca agttcctccg ctaagccaca
    14641 caaccgatta atttcctcaa ttggcgtata ctcatcaatc tcaaagggaa gttcataatc
    14701 atgaattgca tattcctcgt attcgtcatt taacccaatt cgttctttca cttcatcaaa
    14761 atcgacaggt ggggtaaacc acgcccccac caattcacct tcgttatatt tcccgaggtt
    14821 cgcaatataa acacgcatct gttccatcgt gcttccccct tacgcaccga caattcggtt
    14881 aaacaaactg agtaagacat ccttcacccc agaagcatta aagaccaatc cgaccgcaat
    14941 caaagcaatc accaaaaagc caatcagttt ggaaaactcc cgcttaaatc ctaaaaacaa
    15001 accaatgacc acaattgcca gtaagactaa ggattgggca ttggttaaaa accattgata
    15061 tatggatagg taagttcctg atattataaa tatctcaaac ttttcccccc attcacacgg
    15121 agcgtgcgac tttcaccgca ctccgcgttc catcgtgttt atattgaaaa gtatttatgt
    15181 agtagaccta tatcaagttt aattcttgat tgccagctaa tactctatat ttatttagtc
    15241 ttatgagctg gactttattt agcttcaata gagctatata tttttgtatt gtttcttctg
    15301 ttttagcgtg tattagacgg tgtaccgcct cttttaccaa aataagattt ttataatcgt
    15361 catttccacc agctccagtt ggtacaatat gatgacaatg tatttcatta gcttgttgaa
    15421 attcttccag agttatagca cattttccat gttgagcaga aaatagcgaa attcgattgt
    15481 ctgcgtattc tattgtgcga ttttccaaag ataatttcat taactggtac atcagtaatt
    15541 tatcaacact tagattggtg tggataaatt ttcttccctc tagagtgtag caattcgcta
    15601 aatacacctt tgccattggt tttctgtgtt ggatatatcc aacagggaaa ataggttcgc
    15661 cctcgccagc aacataccga agcattttag atttaccata tcttgttgcc tcctttgtcg
    15721 taagttgtct gcctgttttt ctcaatctgc cccttttgtt tgtacctaat ctgttagtga
    15781 agatagccat tacagctcta ttcagtttca tacaatccag atttacattc gttgcaatct
    15841 gataataatt ttgcatacct aataccattg aattataaag tcttgtttct tctaattccg
    15901 ttttcccttt tgagggagaa gcaattttct ttgcctgtga taccagcttg tctttttggt
    15961 ttttgaattg tttatcactt atatgtgatt taacaactaa cttctttctt ctcggtatca
    16021 ttttaatctt gaaaccaaga aagtcggagt atctttttct aacatttaca attcttgttt
    16081 tttcttgtga tacttccaat tttaatcgtt gttcaatcca ttgagtgatt gctattttgg
    16141 ctttttcagc actttccttg tagcggcaga aaactctaaa atcatctgca taacgaacca
    16201 tatacatttc ctttaacttt gttttcttca tttctttgta agcgttgctt ttacatgggc
    16261 taccgctttt attgatatga accacatagt tttttgtaac aggattttcc tgccattggc
    16321 tttctaccca atggtctaac tcattaagaa caatgtttgc tagtagtggg gatattattc
    16381 ccccttgcgg agttcctttc tctggatatg ttattgtacc gttagtcatt ttgattggag
    16441 cttttaatat cctttttaaa atgaatatca agtgcttgtc ccgtattccc atagcccata
    16501 tctgttttat cagcttggaa tggttcacgt tgtcaaagaa acccttaata tcaaattcaa
    16561 tcacataatg tagctggctg atttgtaggc ggttataggt tgcttttatt gcattttcaa
    16621 ctgacctatt aggtctaaaa ccatagctgt tttcactaaa tttagcttcg cagattggtt
    16681 ctaatacttg ttttatacat tgttgtataa gcctgtccca catacaagga atacctagag
    16741 gtctggtttt accatttggt tttggtattt cttttcgcct cactggctta ggtcgatacc
    16801 catgtggact gcctgcaaca atgtatctta cttttttgac aactgtttct gctggcaatt
    16861 tgccaatatc tttaattatt gtaccgtctg ttcctgctgt tttactgcct gcatttgctt
    16921 tgatatttcg atatgccagt aatatatttt ctctcgatag gataatatcc ataagatgag
    16981 tgaaaatatc tccattctta cttttggcat ataaatcatc aaaggtgttt tgcatttcgt
    17041 aatattcagc gtgtcttaaa tcttctacac acaatgtttc atttttattt ttaggcataa
    17101 ggcttcactc ctttacttga aattgagtga gcttttttat cttactcgta atccttattg
    17161 aaacaacata atacttttct aatatataca cgacttgagg gcattcctcc attctcatta
    17221 caagaatttc ttaggttcgc cctctgcttt tcagcattag ttcaactgct tattgttctt
    17281 cgtcagttta ttctcttgat gagaacccaa tacctttcct cgttccgata atcttatcta
    17341 tacatatata tccttaggtc actagctata agcctgctgg cttgataccg cctgtaacgt
    17401 tatatggcat ttcataacct atattcttac tttgccacac cagctacgat ttctcgtacc
    17461 tttgcatttc tacaaagttc aaatttagac ccgtacattc gctgtttcgt cagttcctta
    17521 tagaacattc taaccataga tattttatag acccccgacc tgttggatac gctacccacc
    17581 tctggatagt tttcgataga agatatatgt tcatcaacta ttacggtatc tttcagccgt
    17641 ctttaccgag cttcaaacat tctgagtttc ctcatacaat gcttgtcgga ggctcagggc
    17701 aggcgtttca gagcgttact tcatcattcc acctgtcaga ttatcagttc tcctaataac
    17761 caaacttttt cgttctttta ttagtgcctt agcttttcac taaggaacga gtcgcacata
    17821 aattttgtcc gaaattcatc ttgtcacttt cctttctttt ccattgctcg atccgctgct
    17881 gcacttgcat tcatcaagca catcacagta acaccaagca tactgccacc aaataccaaa
    17941 aaaatatctt ttacaattcc catgacgatt cctcttttca tataatcaaa ttttcaattg
    18001 ccatactttg ttgctccaaa atctttttat gtcggtctga taagcgtgca ttctgtacca
    18061 tatcctggat ataggttgtc cgattcaatc gatccaactc cttagccatt ttccacgtag
    18121 gggcgacttg tcggcctagc caattcaagg ttcgttcaat ggtatacggt tttggctcgg
    18181 aagttaattg aatctctttc cgattccgtc caataaaacg cccccattgt tcattcaagg
    18241 gccagttggt tcgcctttta ccttctactt tatccgcaaa tcgcaagtaa cgattgataa
    18301 tggaaaacgt ggtgctctca atgctacgac cttttaacaa atcttgaatc gcatgaaaag
    18361 cacggtcatt tttcagtcga atctcaaaac gatttttcgt ctcagtttgg tctaatggaa
    18421 tccctaactt gatatattgt tcatagtctt tttcatacaa acagaaataa acctcactct
    18481 tgagactgcc gatatataag gtattgccca tgccgtcctt ttcatcagcc tttaaaagtt
    18541 ccccggaacg ataactttta aaggtacgaa acaaggagat acattcttcc ttctgacatt
    18601 ttttcgttag ctcaggtata tccaatagcc ccactcgatc attgattgcc aagtccaagc
    18661 gtttcatcac gccaccggcc tttagacaat cctcgaaaaa gtcgtaccag ctacgctttt
    18721 gagcgagtaa aaaagtttca aattgtcggc aacctcgccc ctttaattct aaaagcacac
    18781 ctttatcttc ttcatgagac aacatcacca caatatcacc catgacatac tgttcttgat
    18841 aggagtaaaa ggcataatct tcatggagca tatactcaaa tttcaaatgg agaatttctt
    18901 gaataatttt tcgtgcatcc gttgtgggaa agcgaatcct tacatagtca aacagtaacg
    18961 tcaaaggcaa atcgggatta aagctttcca gttggtcaaa aattttcctc atggttttta
    19021 tagatgcggt tacgccacca ttttctatct gatttaagta cggccgacta agtccacaag
    19081 ccgtagccaa tttattttgt gaaacgccgt attcgttgcg tcggtctttt aatagccgat
    19141 aatctaaatt ccgttgtgcc aagttctcac ctccaaattc aaggtgttaa ctttttcaaa
    19201 tgagttaaca cctatacaaa ttacttacta tctttgatac aacaagcatt cttaatggtt
    19261 gcttacttat tttttgtgtt tttgtacccc cctgttagat acgggggtga gacatcatgg
    19321 tcggctaacg ccgtccatga acaggcacag caccacggag gatttctgct tcgcgaatcc
    19381 tccgcggtca gcgcctaaca gtcagttgtg ttggtttagg ttactcatct gtaataccat
    19441 ttacttccaa ctctgctttc atttctaaca agtattgacg aatatctggt tcttttgctt
    19501 ggtccgcaat ccgctcaaat tctgcaatta cttgatttat ttcaattcga gcatccgtct
    19561 cctcattcat taaatatgga tcgccagcga ctttgacacc attcttgcga atatcaaatt
    19621 ttaaccgtcg caattgttct tctaattctg ggtctgactg aatctttcca atcacccaat
    19681 aaatttctcc aatcaactca tcataaacca tgtacatcca actcttttaa tcttctggca
    19741 atatcaaaaa tgaccggaac tgttacagag tttccggctt gcttatacaa ctgactatct
    19801 gaattgactt gagaatctcg atcaaaagcc caatcgggaa agccctgaag tcgccaacac
    19861 tctctaggtg ttagcttccg aatttgatac tgatccgtga cgatcgcttg ttggtctcct
    19921 gttagtaagg tatgaacact ctgttttccc agacgacctc ggcgacttgt ggaattagga
    19981 aaagccagat tcacactgtc ttctggcttc acctcttgat attctgtttc attttttcga
    20041 atcatgattt ttgtaccacc ttgtttcgtt gacaaggtgg gggcggttcc agtaacttca
    20101 taaactcgat taccttgttc aaactttccc ggtaatagtc ctgctacaga gattccatcc
    20161 cttgctggca gtgcgatttt gattccttca tcacctgtta aggttggcgc tacgccatct
    20221 gaaccataga cttgtccacc cattcctcga ttggaaggat tgatatttac caaatttctg
    20281 atgttttgat cagatgttga agacggtcca atgataggaa atacttgttc ggtacatgcg
    20341 tctcgagaat gtccgataag gaatatccgc tcccggcttt ggggaatgta ggcctttgag
    20401 ttaagcacct accactccac gtcatacccc aatccatcca gcgttcggag gatcgtctcg
    20461 aacgtagccc ctccttcgtg attgagcaat cctcggacgt tctcaaggaa taaaagctta
    20521 ggtcggagaa gagtggtgaa ccgagcgatt tcaaagaata aagtacctcg agtatcggaa
    20581 aatctttgcc gctttcccgc aattgaaaaa gcttggcacg gaaatccgcc gcaaaggatg
    20641 tccactggtc cgagggattg aataaactca tctgatacac ttgtgatgtc atgcatttcc
    20701 acctccttac ttgtatcatg aattgcttta taactacgtc tagcaaattt atcaatttca
    20761 caaaaaccga tacaatgatg gctcgcctgt tccataccta aacgaaagcc accaataccg
    20821 gcgaataaat ctaaaaatct catacattac ctcattcatt gcaaagaaaa aagccaagaa
    20881 cattttgctc ttggctcata cattattaat ttgtcctact attttttatt taatccaact
    20941 aatttccgtt gtttttcatt ggcctgatcg gctacatatt taatcaccgt tttgaactct
    21001 tttgcagtag cattttccga taaatccgca atgctcttat tgtatttctt tacaattttc
    21061 gttacttgtt ctttctcagt catgtaattg aacctccaaa tctttcctac tataccctag
    21121 tggtaaaaat aattctagat tatccgtagc ataatgaact tcggaaatat tattcgtata
    21181 atcttttatt atgatatgaa gcaatacatc tggatgttta ctctttactt ttgcaatgtt
    21241 ctctcttgat ttgaaaaaag cgttaataaa tgcctttttc ggcacatgac gtccctctgc
    21301 aatttcacgt tcttgtgtaa atttccaagc gattttaggg tcttgataaa catagaaaat
    21361 cgtcactcga aaatgttttt tcaacgcccg cgagatattt ttttcagcac ttaatattgc
    21421 aaaagttgca tccagaataa aagaatagcc atgatctaag acatatctaa atgcctgctc
    21481 taccaaccaa gaagatgctt tttgaaagag actagagttt tttccattat aatcaggaaa
    21541 ctgggtgcga aagtaatccg catcaataat cacatggtta tcataattct cagctaatcc
    21601 aatggcaacc tctgattttc cagccccagg actccctgcc ataaaaactg cgtctcgtcc
    21661 aacttcgggg gatttccctt tgataatata agaaataaac ttatccttat ttgcttttgc
    21721 atactctaaa taagaatcat tactttccat attaattatc ccctttcttc ctactatata
    21781 aaagtattat acgttacttg aagtataaag tcagtattga agatcactca atcattcatc
    21841 ttttctaatt tactaattga atctaagaaa tcataccctt ttggtacaag tggcgtataa
    21901 aactcactaa tcacactgcc acctgtatcc acataacctc gacctttgat gtgcttcata
    21961 aagaagtttt tttcgacctc cccaaacatc atggaatatc cgagttcact catacgaccc
    22021 aaagccacgc gaaagttgaa ttgatcgcgg attccgtctc ctaaatattt cgcatccggc
    22081 ctttggcaag ccaaaatcag aaagaaacca gattgacgac ccaacatcac gatttgtttg
    22141 agcttattca aaatcaccgc actttctttc gtcgttaaca tctccatata agccacgtat
    22201 tcatcaaaga ttaaaaagtt gggtggaagt ccaagatagg cataattctc tcctgttttg
    22261 tagttagaca tttctttcat tgccttgcta cgagccatca tacgttcata gaaatcttcc
    22321 acacaagcag aaatttcttc cttttgtgaa taaacatgag gcatcaccgt acctaaatca
    22381 gctaaatctg catttttggg atcgaggata aacaattctg catctgactt caatagtgct
    22441 tcgataatgg tgaggaggaa ataggtcttc cctccacccg tgccaccagc gattaacata
    22501 tggggtaaag aatcataagc ccatacttga tttttcatca atcgcagagt cccattttct
    22561 gccactactt cgtctatccc aattcgattg gcgatcatat catacagtaa agtatactcc
    22621 acataggagt ctttgagttc cttttctact aactcacaat acagtccgct ttctaacttc
    22681 ttttccaatt ttaaaagctg atcttggtat ttcccaagtg aaatttgaac gcgaatagac
    22741 agtaatccat ctttcattcg ataacagatt ttggggaagt aactgatagt ctctctggtt
    22801 cggctactat tcaaatcttt gaaaaagcct tcactttgag tttgtttcac ttcataccaa
    22861 tggttctcta gaatcatccg agctagcttt tgtcgatgcc acaagcttcg ccaatgatca
    22921 agaaaaaagt gaatataaag caatatcata gctcctgaaa aagcgagact tccaagcaga
    22981 catactaaat cttttataga ccaaacgatt tcaatagtag aaagtgatag cttagaaata
    23041 tcaaattgtc taaactcttg aaaataaaag acgctcatcc aaccgatgaa cagagcgagt
    23101 agccagccaa gaacaaagtg ataaaccaag tgctgatctc ctgcgcgtat gcgatgtcct
    23161 ttgtacattt tgatggtttc cacctccaat cgaaaaagcc caataggatt tcattaggca
    23221 atttcaaaaa gttatgctag tcatttactg ctacgaggag gggagttgtt tcgtttcaac
    23281 caattgattt aaaaagcctt caatagcatc cggcatctcc acaccaattt cttccaaaat
    23341 ggattctgcc tgccgacata agttttcatt tacttcgaca actataaaga aagactcttc
    23401 ttctcctaac cagtctagta catcagagtc ttcttcactg tcgctatttt ctccaagtaa
    23461 accttgatgt ataatcagaa acattttttc atcagaggtt aatgctttaa cgagttgttc
    23521 ctcagtcagc tcgccttttc catattgatc gagacaatct aaaatatgtt caacattaat
    23581 ttccataatc ttcacctatt tctttctttc attttggttc gtaggatttc cttgattcgg
    23641 ctgccgcttc aagactaaat catccgcctt aatgaaccaa ttaacggtag ttccttctcg
    23701 aaaaacataa ttggcaacgg tatccacgat tggattgacc aactctacct ctgcatcata
    23761 agcaaactcg cgtaagggta cattagctgg gatactaact tgaatcattc gcccttgtgc
    23821 agaggatttt aaattatagg ttcgttcttt tacctcatta ccaattgttc catcctcatt
    23881 ttgcttccgt acttctcggc gtaatgcgga aaaacgcaac ttcccaaatg ttaaacctga
    23941 atctacgaca ataccttctg ttaatctcat cttcttttcc tcctatgctt tcactaaatc
    24001 atcaacatac aacacgtaat cagtaaaccc acgattctca atctcatagc ctttggcaac
    24061 caaatgggga ttcacggctt ttagcggttg gtcttgatca aaatcctttt caccggcttc
    24121 cgcagctact accacctcaa tatcatcggc ccgttgctta ctggaataca aatgataact
    24181 gcgaccaatc accgtagtat tccgtccata tccttctgtt ataatttctc cttcaccggc
    24241 ataacttaac tttccaaagg tttcagccat atctggcacg acaaatttca attccatctt
    24301 cttttcctct tttcattgtt agttttggat acaaaaatag cgcgactact tttttaggag
    24361 gcggtcgcgc acgttggttc ttaggattta cttgtatcat ctcatcacct tagttccggc
    24421 gattccagaa ataatagccc gccgtcgcaa aaatcaaagt aaaaccaatg aacagtaaaa
    24481 cattggaatt tttctccccg gtttgaggga aggtcttcac tgtgggacta gattcttgag
    24541 agccacttgg aacagttggt gtttccggtt gtttcggcgt acttagtatg gttggaactt
    24601 cttttggtgt taaggtttga gatttttctt tcaaatcttc attgtgtttt ccattgatat
    24661 tcccatcttt atcgtaattg atttccgcaa aagtaaactt ggttccttct ggatattttc
    24721 cggtatctac ttttttcgca agcacagttt tggtgaattc tttatcattt acttcataat
    24781 caatcttgcc agatttccaa atttctttgt ttgtaccatc cggtaataaa gcatacagaa
    24841 ttgtttcgaa agcttctttt gagccatcca gtacatcatg agtaatcgac acatcatcaa
    24901 acatatccac cacatcacca tgagtaaagg tctgagaacc gttttctagg tgggcctttg
    24961 tttggataga aacatggtgt tccactgtac aactgacagt ttgggcttgg ttattcaagc
    25021 taacatcctt cgctactggc ttatcgcctg cttcaaaagc ttccttattt tcatacacat
    25081 agttgaataa gacaatagtt ttatttaaaa cttgttcggc ggttaattta tgctgaattt
    25141 tccaagttcc tgttttctct ttgttcgcca acgttgcgct tgtttctgca atcaccactg
    25201 gtttggcttt ttcatctttt tcttgggcag cttttgtcgc atccacatca atggcttgcg
    25261 ctaccacata ccagtcttct ttgatttcat gtagattgta actcaattta tcaactagct
    25321 cggtagaatc cagagaagtt aattctttgt ttccgtcttt ccaagtcgca agagaagtta
    25381 aactatcttc ttcctcaggc aaatcataga gcattaaacg gttcaaacta acagaaaatg
    25441 ctttgttcgt cagcttctcg taaggaacgg tcaccgtttt aattggttgt ttttggtctt
    25501 gttcggtaat ggtaaagaca tactcactct tcacaaagtc ttctttgttt tccttaaaag
    25561 tagaacggat ttctaatggc gtaatctttt gaaaaccttc tggggcttct acttcttcaa
    25621 gtaaataatc cccataagga agattttcaa acttgccata accatcaaac cctaattgct
    25681 cgttataagc tgtggtcgct tcatcttcgg cacctgtgat ttcattggtc ccttccaatg
    25741 gcgacacttt aaaggtcaga tcattgaagc cagtttcggt agtgcccgca gctgatccag
    25801 caaatttaaa gaaatcaaag ccaaagcgaa tcacttgttc ttttgccgta acatttcggg
    25861 taatcacggc atttttttcg ctatcatcca cctttttgat ggatacagga tactttgttt
    25921 catcaaggct atatccttcg ggcgcttttg tttcttgcca ataatactcg ttgatagcta
    25981 aatgtttaac ggcaacttgg ttcttttcgt ctaaagtcaa agtgaccgtt tcatcagaac
    26041 ctttcgttcc cttcgctaac tctggtttaa aagcctcact ccatttaaca gcttttccat
    26101 ccttagcagt aaacaaggtg tattcagcac cctcaaagac tgccttacct tgtgctttat
    26161 ccccagtatc tttgtcttct ttcgtcaaag tggtttcgcc agtgacttct tgattttgcc
    26221 cttttacgtt actggtaaca ggggctacgg tttgattggc atacttcaat tcgactttta
    26281 caggtttgaa cgtattcacg aacccatgac tagctttagt ttcggtcaca taataggttc
    26341 ccaattccaa cgcattagca atctcttttg gtgtttccgc atgaccgttt tcatccgtgg
    26401 tcatttcttg aacgatttca ccggttggac tgtctttgcg aatggcaaac gtattaccag
    26461 ctaaggaata attgtcattc caaagatcac tccctgtttc aactccgctt ttatccagaa
    26521 tgatttgccc tttttcccgt gcgttttttg aagtcacgta aatggtttcg cctgctttaa
    26581 tggtggtagt catgggggtg gtatcaatcg tataaggcgc cggtactgat ttttcggtaa
    26641 tggttacttt tgtaccatga ggaatctcat ccaatgtagc aatgccttct ttgtcagtag
    26701 tcacgtcttt tgcaggtaaa gtttttccaa agtctaaatg gaaaaccgtt cctggtacaa
    26761 catcacccga ttctttatcg acttttttga tctttaaaga accctccgtt tccacatcaa
    26821 ttttgattgt gtaggtatta ggtttatcaa tcgccccggc cattaaagtc tgttggccag
    26881 cctttttata ggcgacaggt gttccggtac cagcagattt cttaagcgtc agcacaccac
    26941 tttcttttga attggcattt ggggtgatta tcaactgatt accattcaca cgataatcaa
    27001 tgtttgcggc attttccaca acctcatcga actctgacaa attcagatta ttcgtatccg
    27061 tcacagtagt cgattgccct aatactgttt tagccgtact attatggaag gttggtttct
    27121 tctgataatc gtcaatggct tgatttattt ttgcctcaat ggctgcgata tttacggcac
    27181 taccatctga tcgttttatt gagtgaaggg tatacccatt aacttcttgc cagatcatct
    27241 tctgtgccac aatatgagta tctgcatctg tacctacttt tttccataaa acggaaacta
    27301 atttggcttt atcggacata tctggtaacg ggtttttctc atagccagga gtgaactcat
    27361 tgtaaatcgg gactcctggt tcaatacaga aaatatcttg tttaaagccg ttgtacactg
    27421 cataatgcgg aacagtgact tctgacattg gacgaccatc actaaaagtt ccttcaacag
    27481 catattgatg tgctaaatct agttctacgg ttaccgtttg tggatgggta atttcatcag
    27541 caaaagtaat cgtaggagca attgctccaa caattgcttg aacagccaaa ctcaaaatag
    27601 ttaatacaca taatttcttt ttttgttttt tatacatgaa atgttctcct ttcaaattaa
    27661 gcacaaaaaa ataagatgct cgcttgaaca tctttgttta taaagtcttt ttaaaatttt
    27721 tattgactga ata
//
LOCUS       DAZKAS010000024        27605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000024 DAZKAS010000000
VERSION     DAZKAS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.024"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..1214)
                     /locus_tag="ACG8H7_000875"
     CDS             complement(270..1214)
                     /locus_tag="ACG8H7_000875"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFP8982987.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1381..2325)
                     /locus_tag="ACG8H7_000876"
     CDS             complement(1381..2325)
                     /locus_tag="ACG8H7_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP8982988.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2546..3760
                     /locus_tag="ACG8H7_000877"
     CDS             2546..3760
                     /locus_tag="ACG8H7_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8982989.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3782..5797
                     /locus_tag="ACG8H7_000878"
     CDS             3782..5797
                     /locus_tag="ACG8H7_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFP8982990.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5880..7253)
                     /locus_tag="ACG8H7_000879"
     CDS             complement(5880..7253)
                     /locus_tag="ACG8H7_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP8982991.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7279..7563)
                     /locus_tag="ACG8H7_000880"
     CDS             complement(7279..7563)
                     /locus_tag="ACG8H7_000880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8982992.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7565..9580)
                     /locus_tag="ACG8H7_000881"
     CDS             complement(7565..9580)
                     /locus_tag="ACG8H7_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8982993.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9759..10061)
                     /locus_tag="ACG8H7_000882"
     CDS             complement(9759..10061)
                     /locus_tag="ACG8H7_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFP8982994.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10150..11181)
                     /locus_tag="ACG8H7_000883"
     CDS             complement(10150..11181)
                     /locus_tag="ACG8H7_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8982995.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11388..12263
                     /locus_tag="ACG8H7_000884"
     CDS             11388..12263
                     /locus_tag="ACG8H7_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8982996.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12415..13257)
                     /locus_tag="ACG8H7_000885"
     CDS             complement(12415..13257)
                     /locus_tag="ACG8H7_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8982997.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13349..15226)
                     /locus_tag="ACG8H7_000886"
     CDS             complement(13349..15226)
                     /locus_tag="ACG8H7_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP8982998.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15223..16644)
                     /locus_tag="ACG8H7_000887"
     CDS             complement(15223..16644)
                     /locus_tag="ACG8H7_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP8982999.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16647..20036)
                     /locus_tag="ACG8H7_000888"
     CDS             complement(16647..20036)
                     /locus_tag="ACG8H7_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8983000.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20293..21684
                     /locus_tag="ACG8H7_000889"
     CDS             20293..21684
                     /locus_tag="ACG8H7_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983001.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21757..22404)
                     /locus_tag="ACG8H7_000890"
     CDS             complement(21757..22404)
                     /locus_tag="ACG8H7_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8983002.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22412..23098)
                     /locus_tag="ACG8H7_000891"
     CDS             complement(22412..23098)
                     /locus_tag="ACG8H7_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983003.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23104..24177)
                     /locus_tag="ACG8H7_000892"
     CDS             complement(23104..24177)
                     /locus_tag="ACG8H7_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983004.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24399..25073
                     /locus_tag="ACG8H7_000893"
     CDS             24399..25073
                     /locus_tag="ACG8H7_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8983005.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25078..26418
                     /locus_tag="ACG8H7_000894"
     CDS             25078..26418
                     /locus_tag="ACG8H7_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP8983006.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26567..27553)
                     /locus_tag="ACG8H7_000895"
     CDS             complement(26567..27553)
                     /locus_tag="ACG8H7_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFP8983007.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8031 a         5216 c         5026 g         9332 t
ORIGIN      
        1 atcaaatatg aacgaattag atatgaatca ttgtatttgc cgttgcggaa ggatttttta
       61 attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga ttaaatttca
      121 tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc tgtttatttg
      181 cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt taagcagttt
      241 taggattttt agttcatatt cattgtttct tataatacag tgttcaatac ttctcttaaa
      301 gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa ttcaacaact
      361 tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc tttttcgtcg
      421 tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt caagatagct
      481 tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt acgatcaatg
      541 atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc ttgggcaata
      601 cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt atgtgaccat
      661 gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct gcgtgggtca
      721 acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt tgttccagta
      781 ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt caaaatatct
      841 actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat ttcttttacg
      901 atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg accaggtttt
      961 tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc atagccacct
     1021 gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc caatgcttcc
     1081 ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat tccttggttg
     1141 atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac tactttacga
     1201 cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt tcatgaaaag
     1261 tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta ttataatttt
     1321 tgatggaaac gaattcctga atacgcataa cagataaatg acaagtgaaa gattttcttc
     1381 ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa
     1441 taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt
     1501 ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga
     1561 agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc caaaatgagc
     1621 tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag
     1681 catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt
     1741 caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt
     1801 atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc
     1861 gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga
     1921 agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc
     1981 ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgagtagc
     2041 ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc cattaataat
     2101 ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg tatgctcggg
     2161 aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa atagatcccc
     2221 tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat tgatattcat
     2281 tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt
     2341 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
     2401 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
     2461 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
     2521 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
     2581 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
     2641 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
     2701 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
     2761 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
     2821 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
     2881 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
     2941 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
     3001 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
     3061 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
     3121 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
     3181 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
     3241 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
     3301 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
     3361 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
     3421 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
     3481 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
     3541 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
     3601 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
     3661 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
     3721 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
     3781 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
     3841 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
     3901 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
     3961 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
     4021 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
     4081 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
     4141 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
     4201 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
     4261 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
     4321 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
     4381 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
     4441 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
     4501 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
     4561 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
     4621 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
     4681 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
     4741 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
     4801 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
     4861 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
     4921 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
     4981 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
     5041 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
     5101 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
     5161 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
     5221 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
     5281 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
     5341 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
     5401 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
     5461 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
     5521 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
     5581 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
     5641 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
     5701 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
     5761 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
     5821 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
     5881 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
     5941 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
     6001 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
     6061 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
     6121 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
     6181 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
     6241 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
     6301 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
     6361 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
     6421 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
     6481 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
     6541 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
     6601 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
     6661 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
     6721 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
     6781 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
     6841 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
     6901 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
     6961 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
     7021 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
     7081 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
     7141 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
     7201 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
     7261 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
     7321 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
     7381 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
     7441 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
     7501 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
     7561 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
     7621 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
     7681 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
     7741 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
     7801 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
     7861 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
     7921 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
     7981 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
     8041 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
     8101 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
     8161 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
     8221 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
     8281 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
     8341 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
     8401 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
     8461 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
     8521 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
     8581 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
     8641 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
     8701 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
     8761 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
     8821 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
     8881 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
     8941 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
     9001 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
     9061 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
     9121 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
     9181 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
     9241 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
     9301 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
     9361 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
     9421 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
     9481 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
     9541 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
     9601 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
     9661 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
     9721 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
     9781 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
     9841 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
     9901 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
     9961 tctgtttcac cagaattagt tcctgctaaa gccacttctt ctgttttagg ggccattgaa
    10021 cctcttaatc gtttgtgttt ttcaccgtca taatttgtca tgttggttcc tcctagtagt
    10081 tctatacttt ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa cccgattctt
    10141 acaaatatgt catatttttc cttctgattc ttgaatgctt ttgattcctt cttccaaaat
    10201 tttcatttgt aagattgttt cttcatcttt gatgactttt tctgcgccat taactaatgt
    10261 ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc tcggaaatta ctttttcttc
    10321 atgataactc ccttgttcat ctatataggt caatacacca taatgttcgg gcaaatcgat
    10381 accaaagtct tcatgatcag gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt
    10441 tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg aaacttggac gttctttgat
    10501 gcggaaataa ctggatttta tcgaaatttt catatttcca tagaaaaaat ccagatcgaa
    10561 gtaatcattc attcgtcctt tccctaaaag ttgacgtaca tcatagctga tatcatctgg
    10621 ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa gcatgtccat ataaatagct
    10681 gttcgcagta gaaaattcat gcgtattttc tggtacttcc ggtctgaaat aatcataatg
    10741 catttctact tctaaaatat cccctaattt tccgctttcg attacttttt ggactgtcaa
    10801 gaagtcagaa tcgaaccgtc tgttttggta acattggata aatagacctt tttcttttgc
    10861 taatgcaaat aactcttctg cttcctttga cgtttctgca aaaggtttct ctactagtac
    10921 attcttacca tgttcgagtg ccatttttcc atattcataa tgaaattgac taggtgtagt
    10981 gatgacaact aattggatct caggatcatc ccaaagatca gacagctgat cagtatattc
    11041 gatgtcttcg aatgttttcc acactggttc agatggggaa aaaatttttt tgatattgat
    11101 tttgttcgta agttttcttg aaaaaggcaa atgatagcga tttgtactct ttccattgcc
    11161 aagataagcg attgtaagca tgagtggtgc ctccttttat tgatgttttc agtatatcct
    11221 agaaaagcgc agaaagtatt tataatagct attctggtaa aaaagttcag aaaaacactt
    11281 gttatcattc ggtttggtga aattaatgtt caaagtaagg ataaaaatca aaaagatagt
    11341 agcggaacca agtgtttatc agtaagatag aatttggagg tcagatcatg cttattgaag
    11401 aaaaactaac aaaacaagag ttgtttacca cgacagagaa acggattgtc gattacatcc
    11461 gtaggaatat cgaagctgca gtctacatga cgattgaaga attagcaaaa gctacgtata
    11521 cttcccattc agctatcatt cgtttatgca aaaaaatggg attcagcgga tttaaagaat
    11581 tccgtttcga gctggggcgg gaagttcatc aattgattgc tcagttcaat caaacgattg
    11641 atcctaattt tcctttttca agtgaagata gcactcagca aattgcaaag aaaatggcgg
    11701 atttatcgat ccagtcaata aaaaaagcac aattgcagat tgaacatgaa gatttagatc
    11761 aaatcggtaa agtattgaac aaggcacagc gaatctttct ttttgcaaaa ggtgattctc
    11821 aaatcacggc aaggaaattc cagaataaaa tggtcaagtt gaataagttt ttgatcatgg
    11881 ctgaagaata cagtgattct tcttggaatg cagccaattt gactagcgag gattgcgcca
    11941 tttttattag ctacagcggt cgcatccatc attatgaacg aataatgacc tatttgaagc
    12001 atgtagaggc ccctaccttg ctgattaccg gaaatcagca ttcagaaatg gccaaacagg
    12061 ctgatatgtg tcttgttata tcgcaggagg aatatgattt tgctaaagta gcgactttct
    12121 cctctcaaat tgccttcgat tacgtattaa atacgttatt ctcagtgata tattctcaaa
    12181 attttgaaga gaatatccgc aacttgaaaa acaaacaaga attgatgcaa aaaggccttc
    12241 ttaaagaata tcggaaaact taagaaaaga tcctcattta gtaataagaa agatagtgtt
    12301 tgataggctc tctttgatat catcataaaa acaggctgaa gttttctttt tcaaacctca
    12361 gcctgtttgt ttacggaacg attcgaatag atatattata gaaatgagga atagctaact
    12421 tctgtttctt attttgaacg attgacgaaa aagatatata agaaggagga aaatcagtat
    12481 acatgcagct aacaatccat acaggaaaaa tcgtttgttc tcggatactt tctgtacttc
    12541 tttttgaact tcttttgcat gataaggaac ccggtgccct ctgacaagta agcgatgact
    12601 gttgaccata taaggtgtac aagttaggag tgtcacgaaa tcctgccctt tttcgattct
    12661 cagatctttt gtatcagtcg gttctaccgt cttgatctga tcgacttgat aagcgagtgt
    12721 ccgtccattg atttcgatat agaaagaatc acctaaagtc agtttcggta agtctgtgaa
    12781 caactttgct tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat tttctccccc
    12841 cgtaggataa gacgttcctt ccaataatga ggcacctttt tctagcagca cagtattcgt
    12901 ttcatcaaaa attggcaaat ggacattgat ctcagggatc gtcaaaatac cgatcgtatg
    12961 tttctcaaaa taggttctat cctttttcgc aggttctttt ttcttagtaa agggatctgc
    13021 acctggattt ccaccttctt ttgctaattg ctggtttttc tttgtcatgt tttcttggat
    13081 tttagccatg acttcttcat tttctttgac cgcttgttgc tgataatgag agatgatctg
    13141 ctgatccaga taattattca gcgcgtcgct aacaaatgga taagctagta tccctattcc
    13201 tgagagaagc aagaaaagca tcagcagatc tagaagtttc cttttctttg gtttcatttg
    13261 tgttctcctt gtgatgactg tttgataata aaagaaaaag gagcatttgc gtaatccgct
    13321 gctccttttt ccaattcttg caaaaagact aaatctgact gtgcttgcgt ctagcaaagt
    13381 acagtccagc aatcagtaga aggactactc ctgcaccgat atacacatag attcccttac
    13441 cgcctgttga aggaagtgta cctttgtgtt tatttggtat tttttcagga gaaatcaact
    13501 gtcctgctgt tacataagat tgttcatcga tagtaaatgc tacacggttt gttaatggaa
    13561 catatttttc tggcgctttc gtttcttcca gatagtacgt gccatatttt agacctgtca
    13621 catcgattaa accgttactt gtggttgtaa aaaccgttgc tgattctttc gccgataccc
    13681 agctgacggc ttttgtggat gggtcgatcg ataaatattt cgctgtgtca ctatcttgat
    13741 cacgaacgac gaattctgct ccagcaagtg tttggtctga tgtaacgtca ccatctactt
    13801 taacaaatcg tttgccccca gtaacgacat cgactgacgg tggtgtttga tcatttgtat
    13861 gcccgttatc gacatttgct tggttagaaa atcctttggt tggatctgct ttttcgttca
    13921 aatgcatata gtaaacgaat ttcaatgtac cgccaggagt taatgaagga atataattcg
    13981 gatcaactga aaccgtgaat ccgtctgttt gctcagtgac agaataattt actgggtcga
    14041 tttctttatt tccatcatat aaggcataag catacgttcc tgaagaatca ttatcaaatg
    14101 ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt
    14161 ctttatcagc gatacctaat gggatattga cagaaatctc atattgaatt ttttcaccga
    14221 ttgcgacatc ttttccattc aattgaggtg ttgttttatc aactttagac gtatcatttt
    14281 tgatggtctt ttctactggt gtttggctat ttgccaggat ctcaaaaggc gtgattgttt
    14341 gcttttcaat catttccgca ttatcaggag cttttacttc ttctaaatta tattttccaa
    14401 cttctaaatg attaacgatc aactggcctt tttcaataga tgcctcggca aagtcattgt
    14461 tgccgatgtc ataagaatga ccagtaatga aatgtttggc tttggtttga tcagttgtcc
    14521 aagtgtacaa tccatctgtg acactttgga tgtatttttt gacgcttggt gttccttctt
    14581 ctttagaaat aataaattct gctccattta gtgcttcgtt ttcggcagta ccgatttttg
    14641 taactttcaa cgttccatca ttaccgactg tatttttagg gtagagatgg atagtatcta
    14701 gttcttctgt cccgtattta taagagccat ctgcttgttt gatcatctca tatacaggga
    14761 aagctaaaac catgtttgcg gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt
    14821 agactgcatc tttcccattt tgttttttag gtaaagataa agtgacattt ccatcagcat
    14881 ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga ctggactgct tgttttgctg
    14941 catccacgga cgctccttta tctcgttgcg cataaaattc ttgagtgacg ttataaactg
    15001 aaaatgaaat atcggctaat ccttggtatt gatcgaattc gctcatttct ttcccgctgt
    15061 tttggattaa aggatcgggt aaatcagtca ttttcttttt atgaaggatg acttgagctg
    15121 ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa
    15181 ggaaaaggac tcctaacata acgtttattt ttttatgatt tttcattttt tcactcctgt
    15241 tctttttttg ataaacaacc agattgcaaa gcttgcgaat accataccaa caacgcctaa
    15301 aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt cttttttatt
    15361 gactactttc aatatactgt ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa
    15421 cggaatttct gtttcactta aaatataacc agaaggtgct tcgatctctt ttaatcgata
    15481 acttcctgtt ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag
    15541 cgcatctgat tcgttctttg tccacttgta tccgttggct gtttgctgga ggtaatttcc
    15601 tgctttgttt ttaataagga atactgcgcc agctaatcgt ttatctggtt tatttgtctc
    15661 gattttgaca aattgcttac tccctgtata tacagagact ttctttgtac tgacaccaaa
    15721 accaaaatcc agtgttccgt tgttgttgat ttccttgttc gcctgtgctg tactgcttag
    15781 ctgcatctga taagaaatgg tcagtttttt tccagcatat ttttccaacg aagggatcga
    15841 aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca aagccttctg tcagcttttc
    15901 tccattcgat gaaatcgtta aactattagg taaaaacgtt aatgcttcat ccgcactatc
    15961 tgacaattcg aacttttgat agtcaaggat atttacagga attgtcgtat gcaaggaata
    16021 agtgattttt tcaccaatcg taaaatcatt tctaggctct tctaatacct tctcaaaagg
    16081 tggatcaggg tagtcgtttt cttcattttt aggatacaaa tggatagttg aaagtttttg
    16141 attgttttgt ccatgtacag gaagaacaac taccatattc tctgcctttt ctttgacgac
    16201 ttctggtact ttggattcaa tgaaaagata cgctttatct cttttttctt gatctttagc
    16261 ggccagagaa aaagaagcga taccatcttc gttgttaaga gttgtagttg ttgcttctgc
    16321 ggtaaacata ccggaagacg caccgttttt tgcgatctct gcttgtgctt cttctaccgt
    16381 tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac
    16441 accatttaat cctcgatacg tttgtaataa agctttttct tgtccgtcat ttggatgatt
    16501 tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt
    16561 ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga agagacacat
    16621 actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt caaaaaaaga
    16681 taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa acggccaatt
    16741 cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga aatctggttg
    16801 tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg aattgaccca
    16861 tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc tggtgtaaaa
    16921 gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt atctgtttct
    16981 tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa ttttgctcct
    17041 tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga
    17101 ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc cgtgtaaatg
    17161 atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc atatccggca
    17221 ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa actggcaaga
    17281 cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc ttcttttttc
    17341 tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg catcgcatac
    17401 ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca
    17461 tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc gatcttgatt
    17521 tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc tgtcagcgta
    17581 taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc gtcgccatga
    17641 tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc aataagatcc
    17701 ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa ttgtttagta
    17761 tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg taattctttg
    17821 attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga taatatctct
    17881 tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt
    17941 gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc tgcagcattt
    18001 gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc tagattgttg
    18061 tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt agcagatggt
    18121 ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt cctgccgttc
    18181 atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct cacttggtaa
    18241 tggatttgga tttcttggtc ttttcctaaa tagatctgat tgctcttgat ggtatttcct
    18301 tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt gactgaaagt
    18361 gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc attgcttaca
    18421 gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt tgcatgatca
    18481 gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg catacgagac
    18541 tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat gccaagacca
    18601 tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc gataaacgta
    18661 ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct tgagattaga
    18721 gaagtatttc ccggccgatc ttgcgtatta gaaaactgag ttccgtaata attgctgctt
    18781 gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc cgtcagcaaa
    18841 acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc tcttagtgct
    18901 ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac ttgatttttc
    18961 actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc ttcgcttgag
    19021 tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa agtatcgaca
    19081 aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat acttcctgac
    19141 cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt tccacgagca
    19201 ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt tcgtatcgca
    19261 tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg atcattagga
    19321 tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt gccttgatga
    19381 tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt gccctgatca
    19441 tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc tggcaaggat
    19501 aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga ttcttcttct
    19561 atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt ctcatcaata
    19621 atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta aagtgctgga
    19681 tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc caacgtaatt
    19741 ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac atcacttaag
    19801 taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt attcgcattt
    19861 agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc tgaaattgtt
    19921 tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac aaagtttgat
    19981 aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt cgtcattttt
    20041 cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag
    20101 gcgttacata attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga
    20161 atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga
    20221 gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaagga
    20281 aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact
    20341 aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa
    20401 gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat
    20461 cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt
    20521 tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat
    20581 tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat
    20641 caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc
    20701 cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga
    20761 agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat
    20821 aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg
    20881 tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga
    20941 gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc
    21001 ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt
    21061 ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg
    21121 tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa
    21181 ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg
    21241 atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga
    21301 tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta
    21361 tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat
    21421 aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat
    21481 atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat
    21541 cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg
    21601 aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa
    21661 ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat
    21721 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
    21781 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
    21841 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
    21901 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
    21961 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
    22021 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
    22081 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
    22141 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
    22201 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
    22261 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
    22321 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
    22381 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
    22441 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
    22501 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
    22561 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
    22621 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
    22681 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
    22741 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
    22801 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
    22861 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
    22921 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
    22981 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
    23041 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
    23101 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
    23161 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
    23221 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
    23281 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
    23341 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
    23401 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
    23461 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
    23521 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
    23581 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
    23641 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
    23701 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
    23761 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
    23821 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
    23881 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
    23941 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
    24001 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
    24061 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
    24121 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
    24181 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
    24241 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
    24301 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
    24361 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
    24421 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
    24481 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
    24541 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
    24601 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
    24661 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
    24721 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
    24781 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
    24841 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
    24901 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
    24961 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
    25021 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
    25081 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
    25141 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
    25201 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
    25261 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
    25321 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
    25381 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
    25441 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
    25501 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
    25561 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
    25621 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
    25681 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
    25741 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
    25801 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
    25861 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
    25921 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
    25981 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
    26041 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
    26101 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
    26161 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
    26221 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
    26281 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
    26341 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
    26401 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
    26461 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
    26521 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
    26581 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
    26641 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
    26701 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
    26761 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
    26821 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
    26881 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
    26941 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
    27001 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
    27061 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
    27121 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
    27181 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
    27241 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
    27301 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
    27361 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
    27421 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
    27481 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
    27541 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
    27601 ataaa
//
LOCUS       DAZKAS010000025        27532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000025 DAZKAS010000000
VERSION     DAZKAS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.025"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACG8H7_000896"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACG8H7_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP8983008.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACG8H7_000897"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACG8H7_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP8983009.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG8H7_000898"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACG8H7_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP8983010.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG8H7_000899"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACG8H7_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP8983011.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACG8H7_000900"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACG8H7_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP8983012.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACG8H7_000901"
     CDS             7565..8422
                     /locus_tag="ACG8H7_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP8983013.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACG8H7_000902"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACG8H7_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFP8983014.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACG8H7_000903"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACG8H7_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP8983015.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACG8H7_000904"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACG8H7_000904"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP8983016.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACG8H7_000905"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACG8H7_000905"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP8983017.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACG8H7_000906"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACG8H7_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP8983018.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACG8H7_000907"
     CDS             13203..13895
                     /locus_tag="ACG8H7_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP8983019.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACG8H7_000908"
     CDS             13892..14659
                     /locus_tag="ACG8H7_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFP8983020.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACG8H7_000909"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACG8H7_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFP8983021.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACG8H7_000910"
     CDS             16624..16944
                     /locus_tag="ACG8H7_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFP8983022.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACG8H7_000911"
     CDS             16944..17294
                     /locus_tag="ACG8H7_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP8983023.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACG8H7_000912"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACG8H7_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFP8983024.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACG8H7_000913"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACG8H7_000913"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP8983025.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACG8H7_000914"
     CDS             19288..20313
                     /locus_tag="ACG8H7_000914"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP8983026.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACG8H7_000915"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACG8H7_000915"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP8983027.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACG8H7_000916"
     CDS             21391..21831
                     /locus_tag="ACG8H7_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFP8983028.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACG8H7_000917"
     CDS             21834..22436
                     /locus_tag="ACG8H7_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFP8983029.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACG8H7_000918"
     CDS             complement(22495..23601)
                     /locus_tag="ACG8H7_000918"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFP8983030.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG8H7_000919"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACG8H7_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFP8983031.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="ACG8H7_000920"
     CDS             complement(25149..27494)
                     /locus_tag="ACG8H7_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFP8983032.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         8878 a         4853 c         5922 g         7879 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat ta
//
LOCUS       DAZKAS010000026        25194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000026 DAZKAS010000000
VERSION     DAZKAS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.026"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8H7_000921"
     CDS             99..746
                     /locus_tag="ACG8H7_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFP8983033.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8H7_000922"
     CDS             1139..3784
                     /locus_tag="ACG8H7_000922"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP8983034.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8H7_000923"
     CDS             4094..5407
                     /locus_tag="ACG8H7_000923"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFP8983035.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8H7_000924"
     CDS             5397..6050
                     /locus_tag="ACG8H7_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8983036.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="ACG8H7_000925"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="ACG8H7_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="ACG8H7_000926"
     CDS             6787..7872
                     /locus_tag="ACG8H7_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8983037.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="ACG8H7_000927"
     CDS             7869..9761
                     /locus_tag="ACG8H7_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP8983038.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="ACG8H7_000928"
     CDS             9768..10145
                     /locus_tag="ACG8H7_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFP8983039.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="ACG8H7_000929"
     CDS             10299..11081
                     /locus_tag="ACG8H7_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFP8983040.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="ACG8H7_000930"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="ACG8H7_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFP8983041.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="ACG8H7_000931"
     CDS             complement(11996..12055)
                     /locus_tag="ACG8H7_000931"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFP8983042.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="ACG8H7_000932"
     CDS             12447..13109
                     /locus_tag="ACG8H7_000932"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8983043.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="ACG8H7_000933"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="ACG8H7_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            13637..13837
                     /locus_tag="ACG8H7_000934"
     CDS             13637..13837
                     /locus_tag="ACG8H7_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8983044.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="ACG8H7_000935"
     CDS             complement(14091..15770)
                     /locus_tag="ACG8H7_000935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP8983045.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="ACG8H7_000936"
     CDS             complement(15772..15984)
                     /locus_tag="ACG8H7_000936"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP8983046.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="ACG8H7_000937"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="ACG8H7_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP8983047.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="ACG8H7_000938"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="ACG8H7_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP8983048.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="ACG8H7_000939"
     CDS             19959..20156
                     /locus_tag="ACG8H7_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983049.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="ACG8H7_000940"
     CDS             20309..20794
                     /locus_tag="ACG8H7_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP8983050.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="ACG8H7_000941"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="ACG8H7_000941"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP8983051.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="ACG8H7_000942"
     CDS             21671..22198
                     /locus_tag="ACG8H7_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983052.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="ACG8H7_000943"
     CDS             22289..23026
                     /locus_tag="ACG8H7_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP8983053.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="ACG8H7_000944"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="ACG8H7_000944"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP8983054.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="ACG8H7_000945"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="ACG8H7_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP8983055.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8390 a         4103 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DAZKAS010000027        24700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000027 DAZKAS010000000
VERSION     DAZKAS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.027"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(171..953)
                     /locus_tag="ACG8H7_000946"
     CDS             complement(171..953)
                     /locus_tag="ACG8H7_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983056.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1018..1854)
                     /locus_tag="ACG8H7_000947"
     CDS             complement(1018..1854)
                     /locus_tag="ACG8H7_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8983057.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1872..3281)
                     /locus_tag="ACG8H7_000948"
     CDS             complement(1872..3281)
                     /locus_tag="ACG8H7_000948"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8983058.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3295..5118)
                     /locus_tag="ACG8H7_000949"
     CDS             complement(3295..5118)
                     /locus_tag="ACG8H7_000949"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFP8983059.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5961..6287)
                     /locus_tag="ACG8H7_000950"
     CDS             complement(5961..6287)
                     /locus_tag="ACG8H7_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983060.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6333..6695)
                     /locus_tag="ACG8H7_000951"
     CDS             complement(6333..6695)
                     /locus_tag="ACG8H7_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP8983061.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6732..6947)
                     /locus_tag="ACG8H7_000952"
     CDS             complement(6732..6947)
                     /locus_tag="ACG8H7_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8983062.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7074..7409
                     /locus_tag="ACG8H7_000953"
     CDS             7074..7409
                     /locus_tag="ACG8H7_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8983063.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7485..9482)
                     /locus_tag="ACG8H7_000954"
     CDS             complement(7485..9482)
                     /locus_tag="ACG8H7_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983064.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9580..9855)
                     /locus_tag="ACG8H7_000955"
     CDS             complement(9580..9855)
                     /locus_tag="ACG8H7_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983065.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10087..10821
                     /locus_tag="ACG8H7_000956"
     CDS             10087..10821
                     /locus_tag="ACG8H7_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8983066.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10860..12002
                     /locus_tag="ACG8H7_000957"
     CDS             10860..12002
                     /locus_tag="ACG8H7_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8983067.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12097..13488)
                     /gene="sufB"
                     /locus_tag="ACG8H7_000958"
     CDS             complement(12097..13488)
                     /gene="sufB"
                     /locus_tag="ACG8H7_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP8983068.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13493..13963)
                     /gene="sufU"
                     /locus_tag="ACG8H7_000959"
     CDS             complement(13493..13963)
                     /gene="sufU"
                     /locus_tag="ACG8H7_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFP8983069.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(13950..15185)
                     /locus_tag="ACG8H7_000960"
     CDS             complement(13950..15185)
                     /locus_tag="ACG8H7_000960"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP8983070.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15182..16468)
                     /gene="sufD"
                     /locus_tag="ACG8H7_000961"
     CDS             complement(15182..16468)
                     /gene="sufD"
                     /locus_tag="ACG8H7_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP8983071.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16481..17254)
                     /gene="sufC"
                     /locus_tag="ACG8H7_000962"
     CDS             complement(16481..17254)
                     /gene="sufC"
                     /locus_tag="ACG8H7_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP8983072.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17451..18299)
                     /locus_tag="ACG8H7_000963"
     CDS             complement(17451..18299)
                     /locus_tag="ACG8H7_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP8983073.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18323..19009)
                     /locus_tag="ACG8H7_000964"
     CDS             complement(18323..19009)
                     /locus_tag="ACG8H7_000964"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFP8983074.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19002..20036)
                     /locus_tag="ACG8H7_000965"
     CDS             complement(19002..20036)
                     /locus_tag="ACG8H7_000965"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFP8983075.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20099..20355)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20431..20769)
                     /locus_tag="ACG8H7_000966"
     CDS             complement(20431..20769)
                     /locus_tag="ACG8H7_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFP8983076.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20771..21127)
                     /locus_tag="ACG8H7_000967"
     CDS             complement(20771..21127)
                     /locus_tag="ACG8H7_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFP8983077.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21133..22320)
                     /locus_tag="ACG8H7_000968"
     CDS             complement(21133..22320)
                     /locus_tag="ACG8H7_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP8983078.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22511..23356)
                     /locus_tag="ACG8H7_000969"
     CDS             complement(22511..23356)
                     /locus_tag="ACG8H7_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8983079.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23590..24555
                     /locus_tag="ACG8H7_000970"
     CDS             23590..24555
                     /locus_tag="ACG8H7_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFP8983080.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7193 a         5206 c         4215 g         8086 t
ORIGIN      
        1 caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc
       61 aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg
      121 gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata
      181 acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa
      241 tcttttttcc cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact
      301 gcattactga tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa
      361 atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt
      421 ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta
      481 tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta
      541 actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt
      601 ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc actataacca
      661 gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca
      721 tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt
      781 cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt attattaatc
      841 tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact
      901 tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca
      961 ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca
     1021 gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc
     1081 catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg
     1141 ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat
     1201 tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc
     1261 aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac
     1321 cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc
     1381 aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat
     1441 agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg
     1501 attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa
     1561 ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat
     1621 tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt
     1681 ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac
     1741 caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc
     1801 ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt
     1861 caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt
     1921 tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa
     1981 ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa
     2041 taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct
     2101 agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc
     2161 ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat
     2221 gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga
     2281 ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta
     2341 gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta
     2401 atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta
     2461 ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact
     2521 gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct
     2581 tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct
     2641 ttaagattca actgatgata caagtgaatt actctagctt gagctaataa aacatggtga
     2701 ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct
     2761 ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca
     2821 ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt
     2881 aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc
     2941 ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt
     3001 tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa
     3061 gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc
     3121 gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt
     3181 tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat
     3241 aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact
     3301 aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta
     3361 attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc
     3421 agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa
     3481 tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc
     3541 aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc
     3601 tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa
     3661 tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac
     3721 cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc
     3781 gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat
     3841 aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc
     3901 aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc
     3961 accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga
     4021 aggctcagta attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc
     4081 tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc
     4141 cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat
     4201 tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa
     4261 aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc
     4321 gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa
     4381 taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg
     4441 ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc
     4501 tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga
     4561 aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa
     4621 ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat
     4681 agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac
     4741 ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt
     4801 ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc
     4861 ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt
     4921 gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt
     4981 ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc
     5041 acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc
     5101 tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc
     5161 acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca
     5221 tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca
     5281 gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac
     5341 atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta
     5401 taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa
     5461 aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat
     5521 ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact
     5581 tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg
     5641 tatcgataga atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga
     5701 tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc
     5761 aaacatatcc ataataattt tttctttttc atttctttta tattgttata acagtgaata
     5821 acataaaaat tcccgacaat cattgtaata tttttttgtc acctgtatta gtatttaatt
     5881 tataggaaac tataaaaact ccctagtgaa tttacacctt tgggagttcc tgtttggacg
     5941 gtatgcataa tacatttaaa tcaatttaac attaacacct cagcatatgg tatctccaca
     6001 tatatacggt ctttctttgg atagtactca atatttatgt tgatagcact tccattgtct
     6061 aatatagctt tagcccggta tgtgttatct ccattttcat gagtaatcgt cacatcatca
     6121 catgatcgat ttaagttgta ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac
     6181 ttctctaatc cagatgtacc tgtcattttt gaaaaaataa tacctataat agccaagaca
     6241 acaataccta taatgacaaa cttcttgttc atatttactt tattcaactt tttatctcct
     6301 atctccaaaa taatttgctc ttatccctct tatcagtaca ttgacatccc taaaagtaca
     6361 ccaaagattg ctccagctac ccctgctatc atcaaaatcg tacccatttt ctcatctttt
     6421 tctcgatata agtagcccat aataacagca acagcaccaa atacaacagg aataaatgct
     6481 aacgatacta ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca
     6541 tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct
     6601 gttccggttg tctcatctgt ttcttgtagt gctccacaaa actggcaata cttactttca
     6661 tctgttattt ctttgccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa
     6721 ttgcttatta gttaactgtt tactttatta aatacatcag aaaacagctc attcattggt
     6781 acacttaata tctcagaaag catcttcatt tgatagggtt taggaatcgt tctccctgtt
     6841 tgccacgaac ttacacattg ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa
     6901 agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc
     6961 tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatataaa aaacttcaaa
     7021 taccattaca aatacaacaa cttgttgtac tatactgaga tgggggtgta ttaatgaaac
     7081 agaaacaata tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg acacaaaaag
     7141 aattagctga aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa
     7201 cagagcctaa cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc
     7261 attttctaga caatgttgtc gtagattatg aggaagagtt tttggctctc tatcgttctc
     7321 ttagcactga agatgcagtt cgaacaattg actatatgaa gctattaaaa aggcaagaaa
     7381 atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg
     7441 ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt taatacaatt
     7501 gggtcttttc tcttccttac tctccgtcca tcatctcttg ttacacttgt aaattgatac
     7561 agaggaacta tcccagctcc tgataatatc aattctttta ttgtttcatc tatccctgcc
     7621 cctttcgcta aatatattaa aaaactacta cttgctttca cattgtatag tttcttaata
     7681 tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttttatt
     7741 aaagcatttt tcacgctgtt atccccaaca ttacgtaggc gttcacaaac agtgttaata
     7801 agttctttct ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt
     7861 gcttcatcta aaatcaattg tttttccttg ctgagttctg atcgggaatt ttgaggaaat
     7921 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
     7981 tatgtaaaat gaattttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca
     8041 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct
     8101 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
     8161 tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta
     8221 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac
     8281 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca
     8341 tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc
     8401 attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
     8461 ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt
     8521 aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa
     8581 taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg
     8641 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
     8701 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
     8761 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg
     8821 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
     8881 tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct
     8941 gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg
     9001 atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct
     9061 tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt
     9121 atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctac gcttaagtat
     9181 ctactatcat ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg
     9241 acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt
     9301 gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta
     9361 acttctctat agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact
     9421 ggcttctcta ataatttttc ttgatctaca taaattggaa tattaatcat tgatgtttcc
     9481 atttgtgttt cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca
     9541 aactctttat tactatatgt tttagtctta tttgtttact caacctttat accgttccat
     9601 gaaactatca atatcagatt ttttaaatct aatgaccgta tcaattttta taacaggtaa
     9661 cccgtattga gaaacatatt ttctaatcgt tcctactgat acacctagat attcacttgc
     9721 ctgctgatag tccatatact ctagagtgac tccagaaata atgtcttcta gcataggctt
     9781 tagcactgta attatctctt tcacttgttg ttcaacttgt atagaaacaa tgctatcaat
     9841 gatttgtaga gacatatttc cccctccttt cgtgattgct tgaacaaatt ttctaagaat
     9901 tatcttcgta ttttttgctc aaaataaaag tattttgtta ttaaccgatt aatcaaacaa
     9961 aaaatgatta ccagttcatc gacatacaca taatacctcg ataaaaaagg ggtgtcaaca
    10021 gctttgtaaa atttatttgc aacacccctt ttttgaggta tactaatttt aggaggtgtg
    10081 gtcaatatga taaaacgtcc accaataaat tatttagaac gaaaaaagat actcggaaca
    10141 aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt
    10201 aacaacggtc aattaattga caaaaaaact atctacgaat gggagaaggg aacctattta
    10261 ccaataccag agcgattatc ccgaattgct gatttgggaa atatgagtat agaggagtta
    10321 gtttgtggca atgtagaaga atatattttg ggaattattc tttatagaga tagcattgta
    10381 ctagatggta taacatttcc agataaaaac ctgttccagc atcttcgtca acagttccca
    10441 ccagtacact ctaatttaga tacatggctg gatagatatt ccaaattaga accagagatg
    10501 caagaattta ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc
    10561 aatatcttaa aaatagaaga attattcatc aatgctattg tagaagaatt tgacaataac
    10621 attcttttcc tcactagcag tatagaagaa ctactagaaa gaatggtaga tgaatggcta
    10681 cctattcaat tgaaagacat gtcttatccc gaagaagctg tgagagaaat tactgataat
    10741 ataaacaagc tagaacaaac gatttcatca attgggaaaa aatacactaa gaaaaaaatg
    10801 aagggaggtg ataccatcta gtggtaagta ctccttgtaa actaagagga gatgatttaa
    10861 tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac
    10921 acattgccac cgatccacga actggtgaaa aaataaatac tacgagaaga ggttttagta
    10981 gcaaacaaga agctcaaaaa gattatgacg agtacagaca tcaaatatta tacggtgtta
    11041 aaaagaaagc acctgatatg acatttgaag acctgtataa tgaatggata actcatcagc
    11101 gtaccagtgt taaagcttca acaatcgcaa tctcagtaag atacgcaaat aatcaaatac
    11161 ttccagcatt tggtaagctt aaactatcga atatctctgt cccatattgt caaaaagtgg
    11221 tagatgaatg gcacagtaaa tatgaaagtt acgattatat gagaaaacag actgcacaaa
    11281 ttttacgcta cggggttgca atgcaatata ttgacaataa tcctatggaa aaaacacttc
    11341 ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag gagcttaata
    11401 atttactgga tgcttttaaa gattttggta acatgaaaca atacgccttc ttcagattat
    11461 tggcatacac aggtatgaga aagagtgaag tactcgcttt acagtggaaa gacatagaca
    11521 cgtttaataa agaactacat gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta
    11581 taattcaatc tccgaaaaca agagcttcta gacgagttat ctctttagat actgagactc
    11641 tttctatcct caacaactgg aaattacagc aaaaagaaga gtatttaaaa cttggataca
    11701 atactagctc taaagagcaa catgttttta caacagttaa aaacactcta tacattccaa
    11761 acactgttaa tgattggtta agatatattt tgaaaaaata taacttgcct cgtattacac
    11821 ctcatgggtt tagacataca catgctagtt tattattaga agcaggagaa tctgtcaagg
    11881 tagttcaaca acgattaggg catgaaaata gtaaagtgac attagacatc tatgcacata
    11941 ttacaaataa tgcacctaag aaaacaggtc aagactttgc tgatatgatg gctcatcagt
    12001 aaaagagctg gtcaaaatct ggtcatttct agtatacagg caaaacaaaa aagctagaac
    12061 ccttatatat caagggttct agctcctata acaggtttac ccaacactgc cttccatctc
    12121 ataactgatc aatcgattga gttcgactgc atattccatt ggaagttctt tggtgaatgg
    12181 ttcaacaaag cccatgacga tcatttcagt tgcttcagat tcggtcaggc ctcggctcat
    12241 cagatagtag agttgttctt ctgagatttt tgagactttg gcttcgtgtt ctaaagagac
    12301 ttggctgttg tggatctcat tgaatgggat cgtatcggat ttcgaccatt cgtccatgat
    12361 gatcgtgtca cattcgatat gagagatcga acccgcactg tttttggcaa aggttacttg
    12421 tcctcgatag tttacttctc cgccgtcttt tgaaattgat ttagagacga tagagctaga
    12481 tgtatttggt gcattgtgga tcatttttgc acccgtatct tggatttggt ttgctcctgc
    12541 aaaggcgatg gagagcatgg tgcctcgtgc accttttcca tctaagtaaa cacttgggta
    12601 tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat tcaacggttg cgccttcgta
    12661 tgcttttgct cgtttggtta ctaagttgta cacattgtct gaccagtttt ggatcgttgt
    12721 gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg atggcggcat gcaggctgtt
    12781 acttgaataa gtaggtgccg tacaaccttc gacatagtgg atgcttgctc cttcgtcaac
    12841 aatgatcagc gtacgttcga attgccccgt gttttccgca ttgatccgga agtacgtttg
    12901 taatggaaca tcgactttga cgccttttgg cacgtaaata aaggtcccac ctgaccatac
    12961 ggctgaattc agcgctgcta atttgttgtc ggttggcggt acaagttttg aaaagtactc
    13021 tttgaataat tcagggtatt ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa
    13081 cttttcgaat tcatctttca tattatggta cacgacttct gattcatatt gtgcggaagc
    13141 accggctaga taagctcgtt cagcttctgg tataccaatc ttttcaaatg tatctttgat
    13201 tttttctggt acgtcttccc agtcacgagc gggtttgtca cttggttttt ggtaatattt
    13261 gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat gtttgcattg gcattttgtt
    13321 aaatgtttct agagatttca gacggaattc aagcatccat tctggttcgc ctttgattct
    13381 tgacatctca cggatcactt cttctgtcaa gccttctcct gtactgaaaa ccggttcgac
    13441 atcatcgtga aacccgaatt tatattcttc taattctggt acgcccatgt tctcactcct
    13501 cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt ttccattgct tttcctagtg
    13561 ctttccaggc aagtgtcgca catttgatcc gagcgggaaa tttagctaca ccaccaagca
    13621 tagcagcatc gcctaattgg tcgggatctt tgacttcttt tccttggaca agctgtagaa
    13681 agtctacagt taaatgttct gcttcttcta ttgattttcc gatgacggcg tctgtcatca
    13741 tcgaggcact tgccgtactg atggagcagc cactgccgtc aaaggcgata tttttgatga
    13801 ttccgtcttt tacatcgact tgcaattcga tcacatctcc acatgtgggg ttgttcaatt
    13861 cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg cggatgggaa gaatgatcca
    13921 agatcacttg acggtataaa ttgtctaatt tagatagtcc catgttggaa aaactccttt
    13981 gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag
    14041 ctcgcgcgag cagtcgctga tacttcaagg tacttcaata gcggttgcgc acaatgatga
    14101 cctgctctga cggctacccc ttccatgtct aaagctgtcg cgacatcatg gggatgtaac
    14161 ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg ggtcttgtgg accataaaca
    14221 gtcaatccat caattgcttg taattttggc aacacataag cggctagttc ttcttcatat
    14281 cgatggatat tgtccatacc gagtttttgc aaataatcga ttgcatggcc taatgcgata
    14341 gcgccggcga tatttggtgt ccctgcttcg aatttccaag gtagttcttt ccatgtactg
    14401 tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt caacaggttc catttcttct
    14461 aaccattttc gtttaccata taaaacacca atacctgttg gtccgcacat tttgtgtccg
    14521 ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa tggccatgtg cggggcagat
    14581 tgagcaccat ctactacgat gaccccgccg ttttcatgga caagttcagc taattcttga
    14641 acaggattga agacacctaa gacattggat acatgagcaa tggagacgat tttggttttg
    14701 tccgtaatct ttttcgctgc atcctccata tccaggaagc cttcttctgt tagttcgata
    14761 tatttcaaga cagcacctgt tcgttttgcc aactgctgcc acgggataac attagaatga
    14821 tgttccatgt aagagagcac tatctcgtct cctgctttga tatagcggtc gccgaaggtt
    14881 ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct
    14941 tttgcatgga tgaacgcccg tactttttct cttgcttgtt cataatcatg ggtcgcgcgt
    15001 tctgctaacg tatggacacc gcgatgaaca ttggcgttat cgtgttcgta atagtacgct
    15061 aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg cattatccag ataaactaaa
    15121 ggttcatcat tgacgtcttg aaataagata ggaaaatcat tgcgtagctt tttagcatcg
    15181 atcatccatt caacttccct tcgattactt cgactaattc tttccgaact tgttctacag
    15241 ggattgctgt taagacagaa ccgaggaatc ctcgaatcac taagcgctct gcttcttctt
    15301 tatgcaatcc tctactcatc agatagtaca tttcttctgg atcaactcgt cctacactgg
    15361 cagcatgtcc agcagttact tcgttttcat cgatcaaaag aataggattc gcatcaccac
    15421 gtgctttatc agaaagcatc aagacacgac tttcttgttg ggcatctgca ccttttgccc
    15481 ctttcaagat gtggccgata ccattaaagg tcaatgttcc tttttctcgg atcactccgt
    15541 gttgcaaaat atgcccgatc gtatgagggg ctttattggt tactcgtgta tcgattcctt
    15601 ggatttgtct gcccgagctg attgccacga ttttgacttc tgcgtgtgca ccttctcctg
    15661 ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt catgacacca attgcccagt
    15721 cgacagaagc atctcgtaaa atgtgcccac ggcgattcat ataagaagta atattttctc
    15781 ctaattgatc tacggctgaa tatttgattt ttgaaccagc tttagcgatg acttccacga
    15841 tgatattacc tgatgatttt gctacttgtt ctttggttgt ctggaaacgt tccagataag
    15901 aaaattcact atgttcatct gccacgatca acacatgctt gaagaagtgc tcatcggatg
    15961 cgccatcttg aatgaataag gactcgagtg gttcatcgat cacgacattt ttaggcacat
    16021 acaggaaaat accgctgttc ataaaagcta catggagtgc agtcaattgg tcttcgttca
    16081 tgtttactgc ttttttcata taatattctt ggactagttc tggatattcg atcattgcag
    16141 tgaacaagtc ggtaaaaatg actccttttt ctgctaattc cacaggcagt tgttcaaaaa
    16201 tcgtccatga accttgttgg acaagcacag gattgtcttt cattgcatca aatgccggaa
    16261 tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc aaataacgac cagcgatgga
    16321 atttcacccg atcgatatgc ggcaatggca gttcatctgc tttcgctagt gcgtcttgac
    16381 ggaaggttct catccaatca ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa
    16441 gataatcgag ccagtttttt tctttcattc ttgctccccc ttacgcttct tcttctttat
    16501 agtcgattcc taattcttta ctgataccag cgtagccttc tgcttccaga cgtttagcta
    16561 ggtcagcatt ccctgtcatg actactcggc cttccatcat gatgtgaacg acatcaggtg
    16621 tgatatagtt caataatcgt tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc
    16681 gcatttcatt tactccttta gcaacgactt tcaaagcatc gatatccaga cctgaatcga
    16741 tttcgtctaa aattgcaaat gtaggttcta acatcaacaa ttgcaggatc tcattccgtt
    16801 tcttttctcc gccagaaaaa ccttcgttca gataacgttc agccatttct tctggcatat
    16861 ttagtaaagc cattttttca tctaactttt tcaaaaatgc cataacagaa attttgtcgt
    16921 tttcatcacg tttcgcattg atcgctgcac gcatgaattc cgcattcgtg atacctggta
    16981 tctcacttgg atattgcata gctaaaaata agccaagtcg cgcacgttca tctacttcca
    17041 agtctaaaac atttttcccg tcaaacaaaa tttcgccttc tgttacttca tagtttggat
    17101 tgcccatgat agcggcagat aaggtagatt ttcctgtgcc gttaggtccc atgatggcat
    17161 ggatttctcc ggttttcatt gttaggttta cgcctttaag gatttctttt tcctcgatcg
    17221 acacatgtag attttttatt tctaagacag acatgcatta tacccccaaa ttctctttcg
    17281 ttctcactca ttttagacta attgagaatg aagcgcaatg accttcctta ctaaaatcaa
    17341 atatctttta tttattatta aacaattttg atttctaaat tagaataggt ataaaaaaga
    17401 tgagaaataa gccattacag acttatttct catctttttt gttccatacc ttattttgca
    17461 tcagccttta ctggtttaac agagccgccc catttgtctg tgatccattc ttggacttct
    17521 ttgcggtgaa gcacttcaat cagttttttg attttgtcac tgtcttcatc acctttacga
    17581 gtcgcaataa tattcacgta aggagagttg tccttttcaa gcaatacggc atcttctaat
    17641 ggattcaacc ctgcgccgta agcgaagttt gcgttgattg caactaggtc accttcatca
    17701 ttttggtacg tagaagctaa aagtgatggg tcgatcgtat ggttgaactt caagttctta
    17761 gggttttctt caatatcatc gaatgttgct gtttccagat ctactccatc tttgactttg
    17821 actaagccag catcttgtag aattgtgatg atgcgtcccc agtctgattc agaatttgaa
    17881 gtaatgattg ttgcaccatc ttttaattcg gaaacatctt tgactttttt agagtacaag
    17941 cccattggtt cgatgtggac agcaccggca ttgacaaagt catatccttt ttctttgatt
    18001 tctttgttca aataaggaat atgctggaag taattggcat caatgtcgcc ttcttccaaa
    18061 gattggttcg gtaatacata gtcatcgaat ttgacgattt caagatcgat tccttcatct
    18121 ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact
    18181 ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc tgtcgctgtt gttaccgcat
    18241 gctgctaatc ctacagtcag caaaagcgct gctgcaaatc caaaaatttt ctttttcata
    18301 tcctacatcc ccctatatcc tattatcttt tgtctgtttt cttagttaag aaatcaccga
    18361 ttccttgaac gataaatacg attagtaaaa taattaaagt cgcaacgaat gtcactgtta
    18421 gattaccacg ctggtagcct tcctgccaag caagacctcc aagtccacct gcgccgatcg
    18481 cgccggccat cgcagtaaat ccgatcatcg agatcgttgt aactgttaat ccagaaatca
    18541 atgcgggaat actttcaggt atcaagacct tccagatgat ttcccagtaa cttgctccca
    18601 ttgcatctgc tgcttccagt acaccagggc tgacttcacg aaaagcaatc tctactagac
    18661 gggcataaaa tggtgctgcg gataaaacaa gagctggaat ggctgcttgc gcacctagca
    18721 tagtgcctac gattgcttta gtaaatggaa tgatcaaaac catcaggatc ataaatggcg
    18781 ttgaccggaa aatattactg atgatcgata cgattccata aatgattcga gcatacggtt
    18841 tgtttttgcg tccaatagag taaaggatca atcccaaaac taatccgatg atcgtcacta
    18901 agaccattga gattaatgtc atatatagtg tatcaaggat agcctgattc atcgtttccg
    18961 ggttgatttg tttaaaatct agatatgttt ctgaaaagct tttatccacg atggatcacc
    19021 tcaattccta ctccttgttc tttgatgaac acttctgctt gatctaattc tttggtttca
    19081 ccagtcatca agacagtcaa tgaaccaaag gaaccttctt tcacttgttt gatctttcca
    19141 tacacaacgt tgacatcgat gttatagcgt cgaatcgctt gggaaatgac tggctcgttg
    19201 gcatttcttt cgctaaaggt caaagttgcc aaacggccgc ttggattttc tttgatgagt
    19261 tcagctaata gttctgtcga atcagcttct ggttcgatgt cttgttgtac aaagcgtttg
    19321 gttacttctt ttttcggttg tctgaagaca gtcaatacgt ctccttcttc tacgactttc
    19381 cccaactcca tcacagcgac tttgttgcag attttgcgga tcacattcat ttcatgtgtg
    19441 atcaagacga tggtcaaatt caatttctta ttgatcgcta acaataagtc taatacttct
    19501 tctgttgttt gcgggtccaa tgcgctagta gcctcatcac aaagcaggat ctcaggatca
    19561 tttgccaatg ctcgtgcgat accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga
    19621 tatgcttgtc cgcgtccttc taacccgact aaacgcaaca gttcttctgc ccgttcttta
    19681 cgtttgctct ttggtacgcc agctaattcc agcggcagct ggatattttc aagtacagtt
    19741 cttgaccata acaggttgaa atgctggaag atcataccga ttttctttcg gaatgtacgt
    19801 aattctttgt tttttaattt tgtaatatct tgatcgttta cgatcacttc gccgctcgtg
    19861 ggaagctcaa gtccattcag gagacggact aatgtacttt ttcctgcgcc agaatagccg
    19921 acgattccat aaatatcccc tttttcaacg tttagtgaaa cgtcattgac ggcttggatg
    19981 tttccgtttt tcccattgaa tgtctttttg acattcgtta gttggattaa tgacatgcca
    20041 ttcacatctt tctcttatga tttttcaaac aaaaaagcct tcgccctctt gccttaagca
    20101 aaaggacgaa agcttgaact tccgtgttac caccttgatt taccgttact tcgcaataac
    20161 agcctcttcc agtacttgga cttagcttct ttccgtatac tgtcgcgctg taacgggcac
    20221 tcccgtagta agtttgctta cgctcgctta ctctgctcta agaccatctt ccatctcatt
    20281 ttcgttaccc attttcagca aacagggctc tcttcaaacg tccgtgacat gtactcttct
    20341 tttcaaagct ttctcgtttc atataggatt gcttatgatt atagcaactg gaagaatcag
    20401 tgtcaacttt tttcaagtga ttttctgatt ttataattcg tcaaattctt ttggatcaac
    20461 atctttgaaa cttaagatcc acgcattcat ttcatctgaa tcattcaaga tattgatatc
    20521 ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt agcgggctgg caaattcatt
    20581 gaccgctttt tccgcttcaa cttcgatcag aggttcatct ttttttagcg attgtcctgc
    20641 ttttggcaga ttggcaaacg tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc
    20701 aacacagtat tcttttccat taaataagat ccaaaggttg tcctttttct tcaaatgctt
    20761 gcctgccatt ttatatcagt tctccttcat aaattttttc attgaatcca attgcaacaa
    20821 gctggtcctc tcgtaagatc agtggtcgct tgatcagcat accatctgtc gataagactt
    20881 ggctcgcttt tgaagccgta aactcgccta cttgatcttt cagtccaagt tcccgatatt
    20941 tcatcccgct tgtgttgaac aggcgacgga ttggtacttg actcgtttct agccaagtct
    21001 caatcgttga ggccttcggt acttctttga tcatatcaac ggtttgataa ctaacgccat
    21061 gctggtctaa ccacgctttg gcttttttgc atgttgaaca tttcgggtac cagtaaaagg
    21121 tgtacaattt tcttacctcc ttgaagatct tgcaggtgct tcattttggt atctcatcga
    21181 taatatcaac ccgataccga tcatgttccc taaaatcgag gatcctccct gactaatgaa
    21241 aggcaatggg atccctgtta aaggaagtaa tccgatattt gctccaatat tctcaaagac
    21301 atggaacagc agcatcatga tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt
    21361 atcgaaacac acccgaatca tacgatagat taaaataaaa tacaagaaaa tgacgaatgc
    21421 accaccgata aagccgaagt tttcaccgat aacggtaaaa atcatatcgg actcacgaac
    21481 aggcacatag atattgctta cgttgaatcc tttaccaaac aagccaccgg taccaattgc
    21541 caaaagtcct tgggccggct gataactcat tcctgacgta tcatggaaag ggtccagcca
    21601 tgaatcaata cgtgcaaact gataagattt gaatccaact ttataaagca agtctcttcc
    21661 gccttcagtc gtaaccaaga agatcgttcc tgctccaacg actactgcca agattactac
    21721 aggtgcgatg atgcgccaag agattccaga catcaggaaa atcccgccaa agatcgcaag
    21781 aaagacaagc atcgttccaa agtcatcttg ggccatgatc aatgctaaaa ccggtattgt
    21841 cacgatcaag agcttcccaa tcagccaaaa gtccgatttc atcgtccgtt cttttacttg
    21901 cgtattatgt ttagttacga tcagtgccat catcaagatg tacgcaatct tcatcaactc
    21961 tgctggctgg aaagtaaacg caccgaatcg aaaccagttt ttagaccctg ttgaagcagc
    22021 taaattctga tcatagaatt tgagcaagag tcccattacc actaacccca aaccataaag
    22081 gtaaggagtc agtttccaga tccatttgga attcaaatgc atgatgacaa cgattgctgc
    22141 cgctccaact aaataccaga caacttgcat ccctacgcct ttcaagactg ctggattgtt
    22201 cggatcatgg ctaagtgcta catataatga gagcagacca atcaaacata acagaaagac
    22261 tggtaaaatc acgccataat caatacgatt atcgttcgaa gcttttaatt ttttttccat
    22321 gtttcatccc ttttcttaaa aaccatactg ctgattatta tagcggtcca gttagataat
    22381 ggaaagtctt tccatagaca tttgggaaaa attaactttc cgtttgtgca gttgcttgag
    22441 caaatgcagt acaaaagcct actgcttatc tttagtgtat catagaactt tttttgatac
    22501 atgcaattat ctaccagctg agattctcct cgctgtgaga agtttctgtg ctttcattgt
    22561 atcgaaggat ctcttcggca atctctagca gctgatcaga aaccagattg gttcgttgga
    22621 tgaaataaaa atatcgatgg aaattcaacg gagtcattgg cacattttct ggaacagagc
    22681 gcttagacaa aatcgagcga ccgattcctt ccttcagcaa cgcaacaatt acatcgttac
    22741 ttttcacgat catcttctta tcttggatat tcatctcttc aaaataacgc tgcgtgtaat
    22801 gaaaaacacc agagtttgct tctcttacaa gccacaattt gctttttggg tccccagcga
    22861 tgactagctg gtcttccatc aaagagtatc tttgtacgcc ggaagtctct aatggctttt
    22921 cgataaaacc aaaatcggat tcatgtttcg ctacttgttc taatgattca tgagaattcg
    22981 tgatttcgat ctcaaaatgc actttaggaa accttttgat cagatgtttc atcaactgag
    23041 gcaaaagata gacagcaaag gtgtgggaag caacgatccg acatacttct tcttcatttt
    23101 gttcattgag caatttttgc tgagtttctt cccattcttc taacaaaacc agtgtccgtt
    23161 catataataa gtcggcttgc ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa
    23221 gatcgatatt caattcccgc tccaactgtt tgatttgatt cgagactgct ggttgagata
    23281 aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg
    23341 ttaataactt aaacatggca ccacttccat aactttttat gataattact ataaaaacta
    23401 tttgatttaa atatacaaaa aaccatctat aatagaaatg tacaacaaaa cagttgctga
    23461 tccaactagt aacttagatg aagacaatca gctgtctgct tgctcgtaat catacattcc
    23521 tattcatttt acaatgtatt cgttcatctt ggtactaaaa tctattaaat aaaaaggaga
    23581 atattatata tgaagaactt tttgaaaatt attccgatcc cgatctgcgg gttgatcctt
    23641 gggttagctt cccttggaaa tctgtttaaa gactatcacc atgttgcttt aggcaatcta
    23701 actggcggta tctctatgat tcttatgata ttcatttttg ttaaattgtt tatcttattc
    23761 gaacacacga aacaaacgtt gcatgatcca atcatcgcct ccgtttcacc aacttttacg
    23821 atgtcactca tggttatttg tacttatttt gtttcatatg aaagtattgc accaattgtc
    23881 aaattcattt ggctgtgcgc tgttatcttc caagtgatcc ttgtctttta tttcaattat
    23941 caccatgtta tcaaagctga tctttcaatt gaagcaattt atccaagctg gtttatcatt
    24001 tacgttggat ttggggtcat cactgtaact gccgggaact tcttcccact tattggaaaa
    24061 attttctttt ggatttcatt gatctgctat gcgttcttat tgccgatcat catcaaacgt
    24121 gtctttttcg taagaaatat ggctcatccg acacgtccgc tgattacgat cgttgcagca
    24181 cctggttctc tttgcctaac aggttatctt aaaaactttg ctgaacctaa tttttatctt
    24241 gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca
    24301 cgtttgttca agcttcgttt ttatccaagc tatgcagcct ttacattccc gcttgttatc
    24361 tctgcaacag ctatatttac tactgttcat tacttcaaca gtttaggtat ctatagtagc
    24421 gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt
    24481 cttgctcatt acgtacgttt cttaattaaa gaacatagca aacatggaaa atttgtttct
    24541 aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa agctgttctg catcaagtaa
    24601 taagaacggc caaacaaaaa cagctgttca tgtttttgta ctggttgggc ttattaccgc
    24661 agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa
//
LOCUS       DAZKAS010000028        23830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000028 DAZKAS010000000
VERSION     DAZKAS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.028"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG8H7_000971"
     CDS             complement(54..788)
                     /locus_tag="ACG8H7_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFP8983081.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG8H7_000972"
     CDS             1115..1525
                     /locus_tag="ACG8H7_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP8983082.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG8H7_000973"
     CDS             1518..2219
                     /locus_tag="ACG8H7_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP8983083.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG8H7_000974"
     CDS             2219..3805
                     /locus_tag="ACG8H7_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983084.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG8H7_000975"
     CDS             3824..4045
                     /locus_tag="ACG8H7_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP8983085.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG8H7_000976"
     CDS             4042..4803
                     /locus_tag="ACG8H7_000976"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFP8983086.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG8H7_000977"
     CDS             5183..5692
                     /locus_tag="ACG8H7_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFP8983087.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG8H7_000978"
     CDS             complement(5767..6306)
                     /locus_tag="ACG8H7_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFP8983088.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8H7_000979"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8H7_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP8983089.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG8H7_000980"
     CDS             7319..8170
                     /locus_tag="ACG8H7_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP8983090.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG8H7_000981"
     CDS             8198..8833
                     /locus_tag="ACG8H7_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFP8983091.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG8H7_000982"
     CDS             8853..9626
                     /locus_tag="ACG8H7_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8983092.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG8H7_000983"
     CDS             10034..10831
                     /locus_tag="ACG8H7_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFP8983093.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG8H7_000984"
     CDS             10809..11222
                     /locus_tag="ACG8H7_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP8983094.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8H7_000985"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8H7_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP8983095.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8H7_000986"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8H7_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP8983096.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG8H7_000987"
     CDS             15999..16559
                     /locus_tag="ACG8H7_000987"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFP8983097.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8H7_000988"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8H7_000988"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFP8983098.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG8H7_000989"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG8H7_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFP8983099.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8H7_000990"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8H7_000990"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP8983100.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8H7_000991"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG8H7_000991"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP8983101.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8H7_000992"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG8H7_000992"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP8983102.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG8H7_000993"
     CDS             21906..22484
                     /locus_tag="ACG8H7_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8983103.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG8H7_000994"
     CDS             22536..23018
                     /locus_tag="ACG8H7_000994"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFP8983104.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG8H7_000995"
     CDS             23135..23509
                     /locus_tag="ACG8H7_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983105.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6649 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DAZKAS010000029        23417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000029 DAZKAS010000000
VERSION     DAZKAS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.029"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1710
                     /locus_tag="ACG8H7_000996"
     CDS             49..1710
                     /locus_tag="ACG8H7_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8983106.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1780..2127)
                     /locus_tag="ACG8H7_000997"
     CDS             complement(1780..2127)
                     /locus_tag="ACG8H7_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFP8983107.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2211..3152
                     /gene="opp3b"
                     /locus_tag="ACG8H7_000998"
     CDS             2211..3152
                     /gene="opp3b"
                     /locus_tag="ACG8H7_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP8983108.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3153..4196
                     /locus_tag="ACG8H7_000999"
     CDS             3153..4196
                     /locus_tag="ACG8H7_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983109.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4202..5272
                     /locus_tag="ACG8H7_001000"
     CDS             4202..5272
                     /locus_tag="ACG8H7_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983110.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5262..6215
                     /locus_tag="ACG8H7_001001"
     CDS             5262..6215
                     /locus_tag="ACG8H7_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983111.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6227..7237
                     /locus_tag="ACG8H7_001002"
     CDS             6227..7237
                     /locus_tag="ACG8H7_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP8983112.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7522..8343)
                     /locus_tag="ACG8H7_001003"
     CDS             complement(7522..8343)
                     /locus_tag="ACG8H7_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFP8983113.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8440..8718)
                     /locus_tag="ACG8H7_001004"
     CDS             complement(8440..8718)
                     /locus_tag="ACG8H7_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983114.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8986..10995
                     /gene="metG"
                     /locus_tag="ACG8H7_001005"
     CDS             8986..10995
                     /gene="metG"
                     /locus_tag="ACG8H7_001005"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP8983115.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11474..12061)
                     /locus_tag="ACG8H7_001006"
     CDS             complement(11474..12061)
                     /locus_tag="ACG8H7_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983116.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12236..13558
                     /locus_tag="ACG8H7_001007"
     CDS             12236..13558
                     /locus_tag="ACG8H7_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP8983117.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13713..14489
                     /locus_tag="ACG8H7_001008"
     CDS             13713..14489
                     /locus_tag="ACG8H7_001008"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP8983118.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14482..15051
                     /gene="rnmV"
                     /locus_tag="ACG8H7_001009"
     CDS             14482..15051
                     /gene="rnmV"
                     /locus_tag="ACG8H7_001009"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFP8983119.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15044..15928
                     /gene="rsmA"
                     /locus_tag="ACG8H7_001010"
     CDS             15044..15928
                     /gene="rsmA"
                     /locus_tag="ACG8H7_001010"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP8983120.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16379..17008
                     /locus_tag="ACG8H7_001011"
     CDS             16379..17008
                     /locus_tag="ACG8H7_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983121.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17132..18241)
                     /locus_tag="ACG8H7_001012"
     CDS             complement(17132..18241)
                     /locus_tag="ACG8H7_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983122.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18450..18872
                     /gene="mgsA"
                     /locus_tag="ACG8H7_001013"
     CDS             18450..18872
                     /gene="mgsA"
                     /locus_tag="ACG8H7_001013"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP8983123.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19059..19157
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19195..19731
                     /locus_tag="ACG8H7_001014"
     CDS             19195..19731
                     /locus_tag="ACG8H7_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP8983124.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19771..21534)
                     /locus_tag="ACG8H7_001015"
     CDS             complement(19771..21534)
                     /locus_tag="ACG8H7_001015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983125.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21537..23252)
                     /locus_tag="ACG8H7_001016"
     CDS             complement(21537..23252)
                     /locus_tag="ACG8H7_001016"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983126.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7541 a         4289 c         4770 g         6817 t
ORIGIN      
        1 tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa
       61 tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta tggcggcagt
      121 tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg cgtatttaac
      181 ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga tacaatcagt
      241 tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga cagcaaacca
      301 cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac ttacaaaatc
      361 aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc ggattacgtt
      421 tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta cctgtatgcg
      481 ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga actagggatc
      541 aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc atatttccaa
      601 tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga agaaaatggg
      661 gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac attagaagat
      721 tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca ttactgggac
      781 aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca
      841 ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga gttagcacag
      901 caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag ctatatcgaa
      961 ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa agctatttct
     1021 tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc agttgtctct
     1081 acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa
     1141 gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg
     1201 aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga tgatgattca
     1261 tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca
     1321 gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc gggtgatttt
     1381 gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt tactgatttg
     1441 tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata tgacgaagca
     1501 gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga tgatttccaa
     1561 aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa
     1621 ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg
     1681 gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg
     1741 aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca aagaaaaaag
     1801 catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa agctgtacat
     1861 accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt tccttgttcg
     1921 tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa
     1981 accgccgata atcagaaacg gcaatgacca taagaataga tcattagata attgaatgag
     2041 tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta gaaaagctac
     2101 tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt tcattatcta
     2161 ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta gtgaatagtt
     2221 atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa
     2281 caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat caggaacgtt
     2341 tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa ccagttatcg
     2401 tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt tccttccaat
     2461 ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc ttgcagttag
     2521 ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg atctctgcca
     2581 tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta ggtcgttcca
     2641 ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa ttacaagttt
     2701 tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt gcgttggcca
     2761 tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa gtactgcata
     2821 gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc gcctttaaac
     2881 atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta gctgtagcat
     2941 tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc ggggaacaat
     3001 tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg atcctttact
     3061 catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta gttgatccaa
     3121 gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag cgttatgaaa
     3181 cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc gaagaagaac
     3241 gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga
     3301 aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg atctctgtga
     3361 tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat atcaatttgc
     3421 ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg gttggtggtg
     3481 aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa
     3541 cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga atctcgttgc
     3601 tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac gggttggttt
     3661 ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa atcttatcag
     3721 gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca ggaatttttt
     3781 caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc gttcgtgcac
     3841 aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta ggtgaatcaa
     3901 gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt attattcaaa
     3961 tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt atcggtttag
     4021 gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac aagacattcc
     4081 tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc atgattggct
     4141 ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac
     4201 gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt atgctggaaa
     4261 ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat tggcgatcgt
     4321 tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct tgctgtcaag
     4381 caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc
     4441 agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc aagatccaat
     4501 gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc ttagaaagca
     4561 taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta aattagttgg
     4621 aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag gaggtcagcg
     4681 tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga ttgctgatga
     4741 acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc tgaaagaaat
     4801 tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg tagtagcgaa
     4861 tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg gaacatcgga
     4921 agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt caatgccgac
     4981 attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg atttattgaa
     5041 cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga
     5101 acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt ggctacttgc
     5161 tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg ctatctttaa
     5221 agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat aaactacttg
     5281 aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta catgctgtaa
     5341 atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga gaatctggaa
     5401 gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact gatggtgaga
     5461 tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg acgaaattcc
     5521 ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct cgtatgaaag
     5581 tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca tctgaagagc
     5641 gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc cacggtactc
     5701 gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca cgagcattgg
     5761 cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat gtgtcgatcc
     5821 aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg acctatctat
     5881 ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt gtaatgcata
     5941 acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt gttcatcctt
     6001 atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag agaaaacgca
     6061 gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt cgtgaaatcg
     6121 caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca aaaaaactaa
     6181 aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc taaaagcttt
     6241 taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta cttttggatg
     6301 tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc aacaaacagg
     6361 agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat tcttaaaaga
     6421 agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt ttcgcaatta
     6481 tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta
     6541 tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa agttaccaaa
     6601 acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga ttcgctccgc
     6661 tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc
     6721 agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga agacattgat
     6781 ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag aagaaaagct
     6841 gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata gaaagatcag
     6901 gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc tttatctgaa
     6961 acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg tcaaacaata
     7021 tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt tttctattca
     7081 cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata ataaaaaatt
     7141 tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga gccaagtagt
     7201 agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa ctgataggag
     7261 cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag gaatttttct
     7321 tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa aaagttcgta
     7381 cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac atcaaaattc
     7441 tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga atgattaggt
     7501 ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat agatttttga
     7561 aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga gcatagcttt
     7621 cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag caaatacttt
     7681 tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg gctcgtgttc
     7741 cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt caaaagccaa
     7801 agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca cttctccgct
     7861 agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt ccttggcatc
     7921 atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg tgacgaccca
     7981 gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat tttcttttga
     8041 aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag ttgactcatc
     8101 cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc cttttctttg
     8161 gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta cgatatcgac
     8221 agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt taagaaattc
     8281 ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag caaaacggtt
     8341 catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc agactctgaa
     8401 aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa aaataaatgg
     8461 aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat accgctcttc
     8521 acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac gcaaaatcca
     8581 agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat acatacgaat
     8641 ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa
     8701 aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt ctttattata
     8761 ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca aaacaaaaac
     8821 ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga tagaagtcgt
     8881 ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta tggtatacta
     8941 gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc agaaaaggaa
     9001 actttttata tcactacgcc aatctattat ccaagcggca aacttcatat cggcaactcg
     9061 tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg ctttgatgtg
     9121 ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc agaagaacta
     9181 ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa attatggaaa
     9241 acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca taaagcagct
     9301 gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg tgaatatgaa
     9361 ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc agaagtgtac
     9421 aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga agtagaattg
     9481 gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct attggaatac
     9541 tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat gatcaacaac
     9601 tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac ttggggagta
     9661 ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt atccaattat
     9721 attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata ctggcctgca
     9781 gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg gccaatcatg
     9841 ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg gctattgatg
     9901 aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa
     9961 cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat
    10021 ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta
    10081 gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg actcgttcct
    10141 gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc tgctaacgtt
    10201 gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt ggctgaaatc
    10261 tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg ggtattagca
    10321 aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc tgaatctttg
    10381 cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa gatcttcaat
    10441 caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg cgaatttcct
    10501 tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa
    10561 gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga agatacagtc
    10621 aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat caaatttgat
    10681 gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa agtagaaggg
    10741 gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg tcaaatcttg
    10801 tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt
    10861 gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat gatcctttct
    10921 gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat gccaaatggc
    10981 tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata cccccttttt
    11041 ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc aatcatataa
    11101 tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag cttcgctgat
    11161 ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa gcataatgat
    11221 aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt ccttacattc
    11281 ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag tagcgttgat
    11341 gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta tatgtgtgat
    11401 tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt ttctttattt
    11461 tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc aaacggcttg
    11521 gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc
    11581 cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc aaccctaaaa
    11641 gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta agtgccatca
    11701 ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag ttctttgtaa
    11761 atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa
    11821 gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct ttgatcaatg
    11881 caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt gcaacagtca
    11941 ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt tctttacggg
    12001 aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc ttaaaatcca
    12061 taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa aaaagaaata
    12121 catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg taaattaaaa
    12181 tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga tgggaatgaa
    12241 aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt
    12301 gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc agtctacaag
    12361 tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg gattgacagt
    12421 ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata ttgaagctgc
    12481 gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt actttcctgc
    12541 taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat tacaattatc
    12601 taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag ggttgaattt
    12661 gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta aattaatacc
    12721 aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg tccagttatt
    12781 tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg cgttgttagc
    12841 aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg aaatgaaacg
    12901 gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa cagtcgttta
    12961 tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg ccggtaattt
    13021 gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa agctggtcac
    13081 tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa ctgggatccg
    13141 cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc tgacaaagct
    13201 ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag tagcttgtat
    13261 tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg tgatgtggct
    13321 attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc
    13381 acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc tcggtgctat
    13441 tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg gtataggtgt
    13501 taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag aagagtaaaa
    13561 tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg tgctttttct
    13621 atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt agtgcttatg
    13681 tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat acccatttaa
    13741 atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa gaaatggggg
    13801 taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa
    13861 gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag gctggcagtt
    13921 atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag gttgtagcat
    13981 taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc caagataaag
    14041 tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt attcatacta
    14101 gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga gatatcggtg
    14161 gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt gacttaggga
    14221 tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc caagaggctg
    14281 cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat ctggcaccag
    14341 ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta gaaaagattg
    14401 ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca aatgcgcacc
    14461 gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat agtcgttgaa
    14521 ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat agaaacaata
    14581 gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga gactcgtgga
    14641 gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat cattatggaa
    14701 gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag
    14761 ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc
    14821 gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga ccttttagac
    14881 tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg
    14941 cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg
    15001 ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg aatatcgtga
    15061 aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt catttaaaaa
    15121 aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg tagaaactgc
    15181 gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg cattgacgga
    15241 acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc gtttgatccc
    15301 tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc aagatatttt
    15361 aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc cgttaaaagt
    15421 cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc ttgaatcaga
    15481 tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc
    15541 tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt atatggaagc
    15601 cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg tagattcagc
    15661 gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt
    15721 tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga ataacttgca
    15781 gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct tggaaacagc
    15841 tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg ccgcattaag
    15901 caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag
    15961 ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg tttttcgttt
    16021 gtttgggctt gttaccgcag atgcaagctt ttgaacaccg tttaagaaag aggttgtggg
    16081 cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct catgtttttg
    16141 agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg aacaaggaga
    16201 tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa ttcttatact
    16261 tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga cagttattag
    16321 taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga gagaaaaaat
    16381 gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga tgggggtcat
    16441 tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt tggcgtctca
    16501 tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg ttgcgctagg
    16561 tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag cattgtcaca
    16621 tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta tcgttttaaa
    16681 aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg tgatcggtgt
    16741 gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg gtaatatgcc
    16801 tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg ggattggtgg
    16861 gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa cactcagcaa
    16921 atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag agtcgattca
    16981 agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa ctctcatcta
    17041 gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag ctgcaccaac
    17101 atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact tgttctgttt
    17161 ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt
    17221 tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt tttgtgtaaa
    17281 gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact acggcttcag
    17341 gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata ccgaagataa
    17401 cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca ggaatacgaa
    17461 gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta aagaagttca
    17521 tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca taaacttctt
    17581 tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg tcagccatag
    17641 tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa
    17701 gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt gataatggct
    17761 catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc acacgttggc
    17821 gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga cctaagatct
    17881 ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt aatttcaagc
    17941 caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt tggaaaacca
    18001 tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg atatttagtt
    18061 ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac tttccacaac
    18121 cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta aaatcagtta
    18181 cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc atttctacca
    18241 taatacgttc cacactttct tttttagttt tattaactat ttaaagtata aatgaaagcg
    18301 ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc aatgtgctag
    18361 ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag taaataagta
    18421 tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa
    18481 aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa aaacatgaac
    18541 tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg ccgatccatc
    18601 ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt tcggaggatc
    18661 agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat gaaccagatg
    18721 taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg aatatcggaa
    18781 cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga agaatcgtcc
    18841 atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa aataacgttt
    18901 aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg atataatacg
    18961 aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta aatgtgaaaa
    19021 tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa aataaagcgt
    19081 taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt cgcggtttta
    19141 ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct aaaaatgaag
    19201 aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc attgatggtc
    19261 actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt cacatttatt
    19321 ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg cgcagtttta
    19381 gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg atttacattg
    19441 aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga gatcacttgg
    19501 aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt tttgacacca
    19561 ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc
    19621 ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta cgtaggacgt
    19681 agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata aaaaacaagc
    19741 agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg cttgatagag
    19801 ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt cgacaatagc
    19861 tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc gatgggcaat
    19921 cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt ttttctctgt
    19981 caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat tactgatcat
    20041 cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc cttgaccgcc
    20101 aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat aagaggcttt
    20161 tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa gattttctcg
    20221 caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt tacgtaatgt
    20281 atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt ttgttgtgtc
    20341 ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt ttcctactat
    20401 agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac gctgcttggg
    20461 gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt tgggttccaa
    20521 ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat caaatgctcg
    20581 ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt cgataaatgg
    20641 tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag tgattgacga
    20701 accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag caaggtgatc
    20761 tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt gtcctttttc
    20821 atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt gctgaaaagc
    20881 tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat ccccaataat
    20941 cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac tcacaaggaa
    21001 aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac gcatcataaa
    21061 aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg ccgcagaaat
    21121 tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc cgtgggagtt
    21181 ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt ttcttaattt
    21241 tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt gactgactac
    21301 gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat tgaagcggac
    21361 ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca tcacaactgt
    21421 tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg cataggcagt
    21481 aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt tcataactat
    21541 tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag taattcttca
    21601 tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg tgcttcttga
    21661 atagagcgga cacgttggga gataataatc actgtactgc caagataagt ctgtagcgcc
    21721 ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc taaaatcagt
    21781 aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg tccgccagag
    21841 aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg acggacaaat
    21901 tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc gtctttctta
    21961 ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg gggaacaaga
    22021 gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc gtccattata
    22081 actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt cgttttcccg
    22141 cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa tgtaatattt
    22201 tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa agcgataatt
    22261 gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga attttctgtg
    22321 tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat cacgacgagg
    22381 tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa agcaagaact
    22441 tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag caaacagatg
    22501 atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat attggaaaca
    22561 cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt ttcttttttt
    22621 gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac gtgttcattg
    22681 agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt gatgataaaa
    22741 aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac ttcccagtcg
    22801 atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat caataaacga
    22861 atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg ggtgatcaag
    22921 gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt tttgattagc
    22981 tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta ttggcaaatg
    23041 atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat ttcaatgatt
    23101 tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat taaaggcagg
    23161 acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat ttgcttacga
    23221 tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt tatttgtcca
    23281 caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg aaaagagaac
    23341 aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga atagactatt
    23401 ttttttaaaa aaaagaa
//
LOCUS       DAZKAS010000030        23065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000030 DAZKAS010000000
VERSION     DAZKAS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.030"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..566)
                     /locus_tag="ACG8H7_001017"
     CDS             complement(111..566)
                     /locus_tag="ACG8H7_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983127.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ACG8H7_001018"
     CDS             complement(720..1928)
                     /locus_tag="ACG8H7_001018"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFP8983128.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ACG8H7_001019"
     CDS             complement(2040..2372)
                     /locus_tag="ACG8H7_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFP8983129.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ACG8H7_001020"
     CDS             2520..3395
                     /locus_tag="ACG8H7_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8983130.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ACG8H7_001021"
     CDS             complement(3477..4058)
                     /locus_tag="ACG8H7_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFP8983131.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ACG8H7_001022"
     CDS             complement(4064..5323)
                     /locus_tag="ACG8H7_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP8983132.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ACG8H7_001023"
     CDS             complement(5325..6371)
                     /locus_tag="ACG8H7_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983133.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="ACG8H7_001024"
     CDS             complement(6606..6881)
                     /locus_tag="ACG8H7_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFP8983134.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACG8H7_001025"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACG8H7_001025"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP8983135.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACG8H7_001026"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACG8H7_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP8983136.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACG8H7_001027"
     CDS             complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACG8H7_001027"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP8983137.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="ACG8H7_001028"
     CDS             complement(10709..11344)
                     /locus_tag="ACG8H7_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFP8983138.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="ACG8H7_001029"
     CDS             complement(11410..12813)
                     /locus_tag="ACG8H7_001029"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP8983139.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="ACG8H7_001030"
     CDS             complement(12800..13831)
                     /locus_tag="ACG8H7_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983140.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="ACG8H7_001031"
     CDS             13862..14083
                     /locus_tag="ACG8H7_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFP8983141.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="ACG8H7_001032"
     CDS             complement(14248..14985)
                     /locus_tag="ACG8H7_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFP8983142.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="ACG8H7_001033"
     CDS             complement(15138..16478)
                     /locus_tag="ACG8H7_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP8983143.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="ACG8H7_001034"
     CDS             complement(16715..18238)
                     /locus_tag="ACG8H7_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP8983144.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="ACG8H7_001035"
     CDS             complement(18616..19227)
                     /locus_tag="ACG8H7_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP8983145.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="ACG8H7_001036"
     CDS             complement(19557..20273)
                     /locus_tag="ACG8H7_001036"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP8983146.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="ACG8H7_001037"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="ACG8H7_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFP8983147.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21602)
                     /locus_tag="ACG8H7_001038"
     CDS             complement(20832..21602)
                     /locus_tag="ACG8H7_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFP8983148.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="ACG8H7_001039"
     CDS             complement(21599..21970)
                     /locus_tag="ACG8H7_001039"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8983149.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="ACG8H7_001040"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="ACG8H7_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP8983150.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaa
//
LOCUS       DAZKAS010000031        22463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000031 DAZKAS010000000
VERSION     DAZKAS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.031"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..421
                     /locus_tag="ACG8H7_001041"
     CDS             20..421
                     /locus_tag="ACG8H7_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP8983151.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            415..660
                     /locus_tag="ACG8H7_001042"
     CDS             415..660
                     /locus_tag="ACG8H7_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983152.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(984..1205)
                     /locus_tag="ACG8H7_001043"
     CDS             complement(984..1205)
                     /locus_tag="ACG8H7_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983153.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1367..1439)
                     /locus_tag="ACG8H7_001044"
     tRNA            complement(1367..1439)
                     /locus_tag="ACG8H7_001044"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1404..1406),aa:Lys,seq:ctt)
     gene            1515..1745
                     /locus_tag="ACG8H7_001045"
     CDS             1515..1745
                     /locus_tag="ACG8H7_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983154.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            1789..2958
                     /locus_tag="ACG8H7_001046"
     CDS             1789..2958
                     /locus_tag="ACG8H7_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8983155.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            3532..4101
                     /locus_tag="ACG8H7_001047"
     CDS             3532..4101
                     /locus_tag="ACG8H7_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFP8983156.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            4088..4948
                     /locus_tag="ACG8H7_001048"
     CDS             4088..4948
                     /locus_tag="ACG8H7_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP8983157.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            5138..5842
                     /gene="yycF"
                     /locus_tag="ACG8H7_001049"
     CDS             5138..5842
                     /gene="yycF"
                     /locus_tag="ACG8H7_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFP8983158.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5847..7682
                     /gene="walK"
                     /locus_tag="ACG8H7_001050"
     CDS             5847..7682
                     /gene="walK"
                     /locus_tag="ACG8H7_001050"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFP8983159.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            7679..8995
                     /gene="yycH"
                     /locus_tag="ACG8H7_001051"
     CDS             7679..8995
                     /gene="yycH"
                     /locus_tag="ACG8H7_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFP8983160.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8996..9865
                     /locus_tag="ACG8H7_001052"
     CDS             8996..9865
                     /locus_tag="ACG8H7_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFP8983161.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9920..10729
                     /locus_tag="ACG8H7_001053"
     CDS             9920..10729
                     /locus_tag="ACG8H7_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8983162.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10832..11461)
                     /locus_tag="ACG8H7_001054"
     CDS             complement(10832..11461)
                     /locus_tag="ACG8H7_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP8983163.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            11631..12923
                     /locus_tag="ACG8H7_001055"
     CDS             11631..12923
                     /locus_tag="ACG8H7_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8983164.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12999..13661)
                     /locus_tag="ACG8H7_001056"
     CDS             complement(12999..13661)
                     /locus_tag="ACG8H7_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP8983165.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13886..14170
                     /gene="groES"
                     /locus_tag="ACG8H7_001057"
     CDS             13886..14170
                     /gene="groES"
                     /locus_tag="ACG8H7_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP8983166.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            14221..15846
                     /gene="groL"
                     /locus_tag="ACG8H7_001058"
     CDS             14221..15846
                     /gene="groL"
                     /locus_tag="ACG8H7_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP8983167.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(15935..17074)
                     /locus_tag="ACG8H7_001059"
     CDS             complement(15935..17074)
                     /locus_tag="ACG8H7_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8983168.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(17185..17769)
                     /locus_tag="ACG8H7_001060"
     CDS             complement(17185..17769)
                     /locus_tag="ACG8H7_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP8983169.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(17896..18327)
                     /locus_tag="ACG8H7_001061"
     CDS             complement(17896..18327)
                     /locus_tag="ACG8H7_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP8983170.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(18345..18665)
                     /locus_tag="ACG8H7_001062"
     CDS             complement(18345..18665)
                     /locus_tag="ACG8H7_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8983171.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            18965..19105
                     /locus_tag="ACG8H7_001063"
     CDS             18965..19105
                     /locus_tag="ACG8H7_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983172.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            19403..19660
                     /locus_tag="ACG8H7_001064"
     CDS             19403..19660
                     /locus_tag="ACG8H7_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFP8983173.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(19631..19939)
                     /locus_tag="ACG8H7_001065"
     CDS             complement(19631..19939)
                     /locus_tag="ACG8H7_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983174.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            20017..20763
                     /locus_tag="ACG8H7_001066"
     CDS             20017..20763
                     /locus_tag="ACG8H7_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP8983175.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            20778..20981
                     /locus_tag="ACG8H7_001067"
     CDS             20778..20981
                     /locus_tag="ACG8H7_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983176.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            21086..21385
                     /locus_tag="ACG8H7_001068"
     CDS             21086..21385
                     /locus_tag="ACG8H7_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983177.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            21385..21567
                     /locus_tag="ACG8H7_001069"
     CDS             21385..21567
                     /locus_tag="ACG8H7_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983178.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(21573..22049)
                     /locus_tag="ACG8H7_001070"
     CDS             complement(21573..22049)
                     /locus_tag="ACG8H7_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983179.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            22102..>22463
                     /locus_tag="ACG8H7_001071"
     CDS             22102..>22463
                     /locus_tag="ACG8H7_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP8983180.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIE"
BASE COUNT         7651 a         3713 c         4422 g         6677 t
ORIGIN      
        1 aaaaaggagc atatttatca tgcaacaaaa ttacaatgag aaatctatac aacatcaatt
       61 tgatgcctac tgtaaaaaag tattacgaaa cgaaactaag tctattcgaa aacgaaacat
      121 taacataaca gataatgagg aaccattgac gaattcaaat gaaggtaagt actcccaaac
      181 tcattttgat gaacaagatg tctatttctt atttggaatg gaaatattaa tttcagatca
      241 aaagttatcg gcagccattg atcatttatc ggaaataaga agaaaggtta tcctacttta
      301 ttattttgca ggttttaatg atactgaaat cggtaaaatc ctcaatatga gtacaagtgg
      361 tatctggtat caacgaaaga aggcagttga acaattaaaa atggggtatg acttatggta
      421 gacaacccca aaaaacggca gtatccatta gttcctctat ccattatcca atctgctcta
      481 gaagaagatc caatttcaat caagatgata ctcaaacatt atcatagtta tatagctaag
      541 ttatgtttga caaatggatt taatgaggcg gggcaattta ttacctatgt agatgagtat
      601 atgctaagac agctagaaat taaattgata gaagcgatct taaaatttaa aattaactaa
      661 ctaaaaagtc ccctttccac ttattctgtt taaaagaatc ttctaatagt taaaggatta
      721 gtagattctt ttaaacagaa aacctattga tcattgaaat ttaaatacat tctatcagta
      781 cgttaacact tcaaggagca tattttagtt gcgccatgat ttcttactgt aaagaaggag
      841 cgataaacat tctaaaataa tttttatagt caataaaaag cgatgatttg aagttgggat
      901 aatgatactt ccgtaaggct cgccgtaaac ttggagagag gtgaaattcc tatgtgcttt
      961 tgacaaaagc agctgattaa gaattatttc catatgttga tttcaacatt ttttctatta
     1021 tttttaagac caaattcagt gaggcaaata gcatcatgta aatttagttt ttctattgaa
     1081 cgttctccac tttttagttt ttttatagta ctcaaattta atcctgtatg tttagagatc
     1141 tcatttgcag tatggttttg taaagtttct cggattattg ttatattagc aatttcatta
     1201 cgcataatta tgttcctttc ttatcaaaaa tatcaatgac tacagtgggc ttttagtgaa
     1261 aaaaaggggt actacagtct cttttgaaaa caaaaaaata cagtatatac gttttctaaa
     1321 tgctaagcac caacgtttac actgtattta agggtttggc acccaatggg ctgtgagggg
     1381 ctcgaacccg cgacccgatg attaagagtc atctgctcta ccaactgagc taacagccca
     1441 aattaatact ttgttattct aacacctaaa ataaaaaagt gcaatactta ttatcagaat
     1501 tgagagtgaa ttttatggat tttcaagatt atgaaaaaat atttgaaagt tatccatcga
     1561 tattgaaaat tactgattta actaaaatgt taaatattgg aaaaaataaa gcttataatt
     1621 tgctgaaaga tagatcaata gaaaatataa aaattggagc aaggtacaaa atacctaaaa
     1681 aagctgtcat tgagtatata ttgaaaatac aagatgaagg taaaaatcat gctataataa
     1741 aatagtcaaa atttttgccg atatttcagt ttaagaggag agattgaatt gaaattaaga
     1801 gcaaacataa ttgaaaaaag aggaacatta caagtagttg tatcttataa agactctaac
     1861 ggaaaaatta gacaaaagtg gaaagataca aaattaaaaa agaaaggaaa taaaaaacga
     1921 gcagaaaaaa ttagagatga atttttacta gaattggaaa gtcaattagc tatggaatgt
     1981 gaatgtgcta atccaaattt attgttttat gactatttgc ttaattattt ggaagtatca
     2041 aaaaaacaag tagcatttaa cacatatgtt gggtataagc attacgtcca taatcgaatt
     2101 tacaagtttt tttatcctaa aaaaattaaa ctaaataggt taaagcctta tcatttacaa
     2161 gagttttatc aatatatgtt aaatgataac tgtacagcaa atacggttat tcattatcac
     2221 gcatttatac gcaaagctct tcaagaggct gttattacag aattgataag tacaaatgta
     2281 gcagataaag taagaaagcc aaaaaagaaa caatttgttt ctcaagttta taatcaacaa
     2341 gagatcttga aattacttga cattttgtca gaagaaaaac tctatttggt agttctttta
     2401 acagcatttt atggattgcg aagaagtgaa gttttaggat taaaatggtc tgctattgat
     2461 tttttaaaca aaaaaatcat gataaaccat gtaatcatag aaaatccaga gaatatgtca
     2521 cagttaataa agaaagatca aacaaagaac tcttccagct atcgtacatt acctttggtt
     2581 gaaacgattg aacgagcatt agtaaatcaa gctaaatggc aacaagataa tcgtattctt
     2641 ttaggagatg attatatttt agaagataat gagtacgtat ttactatgga agatggacgg
     2701 ttgatgaagc ctcaatatgt aacgcatcgt ttgagtaagt tgattaagaa aaatggattg
     2761 aaaaaaattc gctttcatga cttgcgtcat tctaatgctt caataatgtt agatagtggc
     2821 cagaatatga aatccattca agaatggctg ggacatgcat cgtattcgac aactgctaat
     2881 ttatacacgc atttaaccgc aggagtaaaa gatcgtgcag cagaagcatt agaaaacgca
     2941 tttgggttta aagaataaaa aaacaaaaaa caatatcttt ggggtatcgc ttcttgtttt
     3001 tagaattttt ttgttggcaa tttgttggca gtatgttggc aatgaaaaaa ttcacaaact
     3061 aaaaacctat ttataaagct ttataaaact tttttcacaa atagagcatt tggttaagag
     3121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
     3181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
     3241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
     3301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
     3361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
     3421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
     3481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
     3541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
     3601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
     3661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
     3721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
     3781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
     3841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
     3901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
     3961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     4021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     4081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgttccaga
     4141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     4201 ggtatataca gataaaattg acattacccc tgaagagatc tatcaacgtt tgccagagga
     4261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     4321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     4381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     4441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     4501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     4561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     4621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     4681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     4741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     4801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     4861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     4921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     4981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     5041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     5101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     5161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgacgt
     5221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     5281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     5341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     5401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     5461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     5521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     5581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     5641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     5701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     5761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     5821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     5881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     5941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     6001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     6061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     6121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     6181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     6241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     6301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     6361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     6421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     6481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     6541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     6601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     6661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     6721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     6781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     6841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     6901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     6961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     7021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     7081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     7141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     7201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     7261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     7321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     7381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtgattttaa
     7441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     7501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     7561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     7621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     7681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     7741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     7801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     7861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     7921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     7981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     8041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     8101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     8161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     8221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     8281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaagaagta
     8341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     8401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     8461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     8521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     8581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     8641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     8701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     8761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     8821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     8881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     8941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     9001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     9061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     9121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     9181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     9241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     9301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     9361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     9421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     9481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     9541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     9601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     9661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     9721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     9781 taaagtggtc caaattgaga gcgtcaacgc tttcagcaat acgatcgtga caaacaatac
     9841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     9901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     9961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
    10021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
    10081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
    10141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
    10201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
    10261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
    10321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
    10381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
    10441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
    10501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
    10561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
    10621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
    10681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
    10741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
    10801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
    10861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
    10921 tcgtactgtc gctagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
    10981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
    11041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
    11101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
    11161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
    11221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
    11281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
    11341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
    11401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
    11461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
    11521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
    11581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
    11641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
    11701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
    11761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
    11821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
    11881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
    11941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
    12001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
    12061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
    12121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
    12181 cagcggtttt tcaacatttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
    12241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
    12301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
    12361 ccgtcttgca agcgaattca ttagcaaatg cagaaatctt agcctcagga caagagttga
    12421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
    12481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
    12541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
    12601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
    12661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
    12721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
    12781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
    12841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
    12901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
    12961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    13021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    13081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    13141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    13201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    13261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    13321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    13381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    13441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    13501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    13561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    13621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    13681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    13741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    13801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    13861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    13921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    13981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    14041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    14101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    14161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    14221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    14281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    14341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    14401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    14461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    14521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    14581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    14641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    14701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    14761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    14821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    14881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    14941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    15001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    15061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    15121 gacgatctag gtttagaatt gaaagatgca acaatcgaaa acttaggaaa tgcttctaaa
    15181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    15241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    15301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    15361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    15421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    15481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    15541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    15601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    15661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    15721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    15781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    15841 atgtagtaat taagaaagaa ccctatttta acagggttct ttcttctttt tttgttttat
    15901 tttaagcaaa aggggcaaag aaggggcaaa aatatcaaag gctatttagt ttatcaatta
    15961 cttgttgttt ggctcttttt gttacatggt tataaataga cagagttgtg tttgcatcag
    16021 aatgccctac acgctccata atggctttaa gaggtacgcc taattcagat aatagtgaaa
    16081 cgtggctatg tctgaatata tgtgaggata gacttttttc taattccaac tcttcctcta
    16141 ctttatggag tatagcgtta aatgagtgca gcgcaagcgg agtgccactt gtagatataa
    16201 atatatattg atcgggatct gtgggtctac ctgcaagaat attgtcagct atcacacttt
    16261 caatcaattc ttttgcacga ttgggtaatt gcacttcacg ttgcgaataa gtattttttg
    16321 gagttgtttt tatagcatta tccattttca cagatgtgta atctaaggtc ccattaatgg
    16381 aaattttccc atcctcatag tccttcattt gcaaagctag caattcccca tatctcaaac
    16441 cagttaaata tagaaactca gctattatgc cgtgtagttt tctgcgagga ttggagtata
    16501 gctgtttcag tatttgatca atttcttctt tctccagata ttttttattc atagaaagcc
    16561 ttcttttttc ttcttccact tttttaggat ggattttaac tgctagcgca gggtttcttt
    16621 gaatgtattt tctatcgatt gcatagttta gcataacaga tagagttgtt tttgtttgtt
    16681 ttgtgtagtt cagtgagagg tcaccaaacg tatacatatc ttcaattatc ttattaatga
    16741 gtgtctcatc aatgtttcta acgatcgtat catcgcttat gtgcttagaa acatgtttca
    16801 tcatcattgg aaccttcaaa tagctagtac gtttaacatg ctgcttataa tattcatacc
    16861 attctttata cagctcacca aaagtgatat ctgatttatt gtaatcttcg agtgcttctt
    16921 taattttttt atctagaatt ttctgagctt ttttccacgc ttgtggtgaa ttacttgtaa
    16981 gtgttgtaga tttttttcgt gttttttctg tataaggatc tacatatctt tcaataaact
    17041 tgaattggcc atctttggtc tgttcaaccc acacttttaa catctcctat catttgctat
    17101 aataggcata acaaatagac ctatataggt ttgttttcta aaagcacgct cttactctgg
    17161 acggtggggc gtgtttttta tttattatac agttgcttta aaaatagctg ctttttcaaa
    17221 tgatactaaa ggagagaagt ggatttctat ttctcccttt tggtttaacg tgaaatgagc
    17281 agttacatcc atttgttttc ctggagcaac agatcccatt gtattttcgt tggcatatgt
    17341 ctctgatttt ttgtcatctg gtccatatac ttccacatct gtacctacag ggatatctga
    17401 atcgccatca ttttttacgg tataagtaat ttttactact tgtgcaggtt ggttttcttc
    17461 aaattgattt ctttcatcag ttagttctac actatttagt gtatattcag catcaccaac
    17521 ttttacagtg tcaccaatct tatagaaggt atcgcttttt tcttctttag aagatgaagc
    17581 agaggttgaa gatttagtta ctttttcgcc accattatca cttgctttat tactatctga
    17641 tccaccattt aaagcagaac caataattat aattaaaatt actgctaata cccaaaacca
    17701 tacttttttg taaaaaggtt tactaacttt atacattttt ccgtcttgac ccataacttt
    17761 ttttgccatt taaatattcc tcacttcttg ttataatata tttgcgatct cagaaatgag
    17821 gtatgagtcc gtgtgccagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa
    17881 taattcttga tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca
    17941 ttggcagaca gcataacaag tgtatctgag cttattctga atagaataat actccataaa
    18001 tttttcgaga ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa
    18061 aattagaata gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt
    18121 atcgtacaaa actgaatatt caaaatgaga agcggtgaaa tgcgcaagtt catgagataa
    18181 atgaaaggct tctgcagttt cgccatatag attttcattc aaaaaaatga ttctaggctt
    18241 cggataatag aagccaggct ctttcatttc catgtaaact actttcaaat tgtattcgct
    18301 caacatttct ttcaatttca aatacataca acccatcact ccaactattc attttcctct
    18361 aaagctttag caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca
    18421 aaagaaaaaa cagtatcgtt ttctgataaa tccacatgtt taggggtttc tctttcttct
    18481 ctacctagaa ggtagtctac agagactcca aagtaatcag ctaaccgagc taactcaata
    18541 gaattaggag tttgtctttt ccaacttcct aaatatccat tcgaataccc gaaattcatt
    18601 tcgagctgtc taatggataa tcctttttct tttgctaact cctttattat ttcgtaagta
    18661 ttcattgata aatcaacctt tctgaatgct tacaaaaaaa gtttagataa ataatgtaaa
    18721 aatagttgac ttatatacat tattaatcta tactatgttt tgtaaacaag tttaacaact
    18781 aaaaagacaa caaaaaatac tattgattaa taaatgccaa ccgccaagaa agctttttaa
    18841 atcaaagttt atatgtctta tttaactatg catttagtat agattattaa tctaataaag
    18901 tcaacgcagt tttttaaaaa gttgttaaat ttgtttacga atataaaaga aaggagagaa
    18961 gaatatggaa aaaacagtct cggcaaaaat cgaagatttg aaattagaca tactgaaaca
    19021 agcaaaagtg gcgatggaac acgcggtaga taaagaagac tctgccatgg ttgcagccat
    19081 agcagagatt ttagctcacg tttagtcatc gtttttacgg tcttctggaa gctgaccata
    19141 gataacagca taatgaatat ctaaataagc ttgaacaatt tcttttggcg aaattgattc
    19201 gcctttagta gatacttgtg attcgtgatt tagccatgca acaactaaat ccgcagcgat
    19261 ttcaaacggc gttacacttt gtgtcagttg ccataaagaa tttcacaata tgtatggtcg
    19321 tggtaataat accaaagagc aatttgagga atttatggaa attaaaaaat acaaaaaatc
    19381 atttattaaa ggaggctaaa caatgaacaa ggaaatgagc caaacaatca aggtacaaaa
    19441 aatgatagat gatctaacac atggaattga tagccaagcc gataaaatca tcaaggaatt
    19501 gcaaggacaa aaagttaagg atgcaaaaat gcttctcaag actatcaact ttgaaatgaa
    19561 tccaacaaaa agaaagctcg ccgatgtgtt ggaagaaaag ttggcttccg ctatcaacga
    19621 gcaagaatta ctatttgaaa ccgatacttt taacagctga tgttttatag atgacagttt
    19681 catccgaatc tatatcatcg ccgagagaaa agaatgttgc ttttgacaaa aagccgataa
    19741 gacctacaag cggtcttgat gttgcaagag tagatacccc aatatcacct tgcaaggtat
    19801 tcacgtaaat ttgagaagaa tagaacattc catctttttc ttcgatattt gacgcatcat
    19861 cgtatccaac gaccaaagta tcggcaaata ttttgatttt ttctccatca agcatagtaa
    19921 ttacaaattc tggtttcata aattttcacc tccttaacaa ttatttcagc ctgtcacact
    19981 gataaggaaa ttataccaaa gaaaggaatg agaaatatga atacaccaca aatttttaat
    20041 ttcgagcaaa acgaagttcg aactatttta gtaaatgatg aaccatattt tgtaggaaaa
    20101 gatgtagcag acgttctagg atattcgaat ccgcaaaaag ctattcgaga tcatgtggac
    20161 ttggaggata agacgcagaa cgattcgttc accgtcaatg gaacagcagt tgttttgatt
    20221 aacgagtcag gcctttacag tttaatctta aaatcaaaac ttccctctgc caaaaaattt
    20281 aaacgttggg taacgagtga agtccttcca acaattagaa aaacaggtag ctattcaaac
    20341 gtacctcaaa gttttgcaca agcattgcgt ttagcagcgg atttagaaga aaagaaccaa
    20401 ttactcgaac aacaaattgc cgagtacgaa ccaaagatta gctacttaga tacgattctt
    20461 tcatcgacag atacggtagc tacttctcaa attgcagctg attacggaat gtcggcaatt
    20521 gctctaaaca aattgctaaa cgagttaggt gttcagcata aagttagcgg acaatggata
    20581 ctttaccgaa aacatatgaa ccaaggatac acaaaatcgc acacaagtga gataccgaaa
    20641 gccgatggcg gcactaaagt tgtaatgaat accaaatgga cacagaaagg gcgagtgttt
    20701 atttacaact tattaatcgc agagggctat taccctcaaa tggatttaga ggaaattggt
    20761 tagaaaggag ttttagtatg actgacattg cagaaatcac tcaacgagat agagaaaaaa
    20821 tcaaagaata tgtcgaaagt tcaaagttct taacttacac catgcttgct gaaagatttg
    20881 gaattagcaa aagctactta tctttaattt taaacggtaa aaagacttct gcagaagcaa
    20941 acagaattat agattcgatt atcactatgt acgaattgta gagggaggta aagtcgttgg
    21001 aagataaaat cattcttaca aaatcagagc ttcaagaaat gttaaacatc gagtatggaa
    21061 cgataatttt taggagggat aataagtgaa aaaaccaaca ctttcagagc tgatagaagc
    21121 tgctgagaag gcagtaaaac cagacgactg gtaccgacaa agtttaatct tggagaagtt
    21181 ccacggcatg tcaaaaacta ctttagttga atactgcaag gaaatggaaa caattcctga
    21241 atttgcagaa ggaatacttc gtccaggtca ctcaaccaca tttattcatt accatacttt
    21301 tatttggttt ttaaagtgga aagacgcaaa caaatatcgt gtaaaaagtt tatcgccttc
    21361 agatgttttg aaggaggcaa gttaatgggc aaattcaaca gagcactagt attcagcgca
    21421 ccgctaatca tctacgcttt aggcctctgg ggaagcagac aagcgttgat agggacaatc
    21481 gtttacatgg tttggatttt tatggggctt gatgaagctg agcacagagc gaaaaaagcc
    21541 agtcgggagg gactgactaa aatatgattt ttttattttt gttcctttgg gctgctattg
    21601 cgtacttcat ccagctcctt gttgtcaaag ttgctcttat ttcttttagg aagaacaaaa
    21661 gaaaatagcg ctataaatga aagataagta gccgctacat taaactgttc ttttgaaaaa
    21721 gttacagtca tcggaatgat aattgatatt gaggaagtaa tcttaattaa aaatatagca
    21781 atattttcaa catcgcgttg gtttttattt aacattgtcc aacatgtttt agcaacaata
    21841 aaaataatta cacatgatat tagcacacag actgaaaaaa gttttagaaa tgcgatgtag
    21901 tcagtgttat tgaactcctt caaaatacta tttttattca atgtaataaa aggaattttt
    21961 ttaattgaag acaggaaaga taaggtaata aacagaatta tatatatggt taaagtcgca
    22021 gtaagaatgt aattgagctt tttcctcata gaatcacctc actttctaac gaaaattata
    22081 ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgta
    22141 actacgagct tacaagtggc agaaagtttc aaaaagaaac ataagcatgt actagaagca
    22201 attgaatcaa taaaaagatc ggtcgaaaat tcggccaatg ttgaagatgg gtccaatttt
    22261 ggacagatgt ttgtggaagg gaacgagcca gactcatacg gaagaagtcg gagagtttat
    22321 ttcatgaata gagacggatt ttccttgcta gctatgggat tcactggaag taaagcaata
    22381 aatttcaaac taaaatttat tgaagctttc aacgaaatgg aagatgttat tcggaagaat
    22441 actgttcctc aaacaattga aga
//
LOCUS       DAZKAS010000032        22031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000032 DAZKAS010000000
VERSION     DAZKAS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.032"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACG8H7_001072"
     CDS             367..555
                     /locus_tag="ACG8H7_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983181.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACG8H7_001073"
     CDS             545..805
                     /locus_tag="ACG8H7_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFP8983182.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACG8H7_001074"
     CDS             846..1073
                     /locus_tag="ACG8H7_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983183.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACG8H7_001075"
     CDS             1138..1710
                     /locus_tag="ACG8H7_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983184.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACG8H7_001076"
     CDS             1711..2022
                     /locus_tag="ACG8H7_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP8983185.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACG8H7_001077"
     CDS             2013..2273
                     /locus_tag="ACG8H7_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983186.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACG8H7_001078"
     CDS             2434..2793
                     /locus_tag="ACG8H7_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983187.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACG8H7_001079"
     CDS             2790..4433
                     /locus_tag="ACG8H7_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP8983188.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACG8H7_001080"
     CDS             4468..5550
                     /locus_tag="ACG8H7_001080"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFP8983189.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACG8H7_001081"
     CDS             5594..6766
                     /locus_tag="ACG8H7_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP8983190.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACG8H7_001082"
     CDS             6753..7457
                     /locus_tag="ACG8H7_001082"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP8983191.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACG8H7_001083"
     CDS             7462..8622
                     /locus_tag="ACG8H7_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP8983192.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACG8H7_001084"
     CDS             8698..8976
                     /locus_tag="ACG8H7_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFP8983193.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACG8H7_001085"
     CDS             8957..9328
                     /locus_tag="ACG8H7_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFP8983194.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACG8H7_001086"
     CDS             9344..9748
                     /locus_tag="ACG8H7_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983195.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACG8H7_001087"
     CDS             9745..10155
                     /locus_tag="ACG8H7_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983196.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACG8H7_001088"
     CDS             10216..10803
                     /locus_tag="ACG8H7_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFP8983197.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACG8H7_001089"
     CDS             10876..11343
                     /locus_tag="ACG8H7_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFP8983198.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACG8H7_001090"
     CDS             11389..11697
                     /locus_tag="ACG8H7_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983199.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACG8H7_001091"
     CDS             11892..18626
                     /locus_tag="ACG8H7_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP8983200.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18657..19502
                     /locus_tag="ACG8H7_001092"
     CDS             18657..19502
                     /locus_tag="ACG8H7_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFP8983201.1"
                     /translation="MNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSFREIGESIEL
                     SEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSLKSLSKTEID
                     SQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYTKNVWEKKGV
                     FKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFINMKDTQILE
                     VSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGHANVEIELYE
                     ERDLF"
     gene            19499..20548
                     /locus_tag="ACG8H7_001093"
     CDS             19499..20548
                     /locus_tag="ACG8H7_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983202.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACG8H7_001094"
     CDS             20552..21376
                     /locus_tag="ACG8H7_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983203.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACG8H7_001095"
     CDS             21394..>22031
                     /locus_tag="ACG8H7_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP8983204.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAZKAS010000033        21843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000033 DAZKAS010000000
VERSION     DAZKAS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.033"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8H7_001096"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG8H7_001096"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP8983205.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG8H7_001097"
     CDS             complement(1172..1867)
                     /locus_tag="ACG8H7_001097"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFP8983206.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG8H7_001098"
     CDS             complement(1880..2596)
                     /locus_tag="ACG8H7_001098"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFP8983207.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG8H7_001099"
     CDS             2926..3534
                     /locus_tag="ACG8H7_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFP8983208.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACG8H7_001100"
     CDS             3859..4182
                     /locus_tag="ACG8H7_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFP8983209.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACG8H7_001101"
     CDS             4364..5734
                     /locus_tag="ACG8H7_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFP8983210.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACG8H7_001102"
     CDS             complement(5864..7165)
                     /locus_tag="ACG8H7_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP8983211.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACG8H7_001103"
     CDS             complement(7519..9510)
                     /locus_tag="ACG8H7_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFP8983212.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACG8H7_001104"
     CDS             complement(9654..9920)
                     /locus_tag="ACG8H7_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP8983213.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG8H7_001105"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG8H7_001105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP8983214.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG8H7_001106"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG8H7_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP8983215.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACG8H7_001107"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACG8H7_001107"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP8983216.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACG8H7_001108"
     CDS             complement(14080..14601)
                     /locus_tag="ACG8H7_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFP8983217.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACG8H7_001109"
     CDS             complement(14604..15446)
                     /locus_tag="ACG8H7_001109"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFP8983218.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACG8H7_001110"
     CDS             complement(15439..16164)
                     /locus_tag="ACG8H7_001110"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFP8983219.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACG8H7_001111"
     CDS             16340..16636
                     /locus_tag="ACG8H7_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983220.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACG8H7_001112"
     CDS             17204..18796
                     /locus_tag="ACG8H7_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP8983221.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACG8H7_001113"
     CDS             18809..19549
                     /locus_tag="ACG8H7_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP8983222.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACG8H7_001114"
     CDS             19717..21066
                     /locus_tag="ACG8H7_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8983223.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACG8H7_001115"
     CDS             complement(21194..21784)
                     /locus_tag="ACG8H7_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP8983224.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6273 a         4456 c         4149 g         6965 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aac
//
LOCUS       DAZKAS010000034        21737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000034 DAZKAS010000000
VERSION     DAZKAS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.034"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACG8H7_001116"
     CDS             complement(152..550)
                     /locus_tag="ACG8H7_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP8983225.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACG8H7_001117"
     CDS             complement(547..1320)
                     /locus_tag="ACG8H7_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP8983226.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACG8H7_001118"
     CDS             complement(1317..2000)
                     /locus_tag="ACG8H7_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFP8983227.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACG8H7_001119"
     CDS             complement(2167..3054)
                     /locus_tag="ACG8H7_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8983228.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACG8H7_001120"
     CDS             complement(3051..3920)
                     /locus_tag="ACG8H7_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8983229.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACG8H7_001121"
     CDS             complement(4057..5343)
                     /locus_tag="ACG8H7_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8983230.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACG8H7_001122"
     CDS             5616..6194
                     /locus_tag="ACG8H7_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8983231.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACG8H7_001123"
     CDS             6194..7123
                     /locus_tag="ACG8H7_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983232.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACG8H7_001124"
     CDS             7120..8856
                     /locus_tag="ACG8H7_001124"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8983233.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8H7_001125"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG8H7_001125"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFP8983234.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACG8H7_001126"
     CDS             complement(9752..10261)
                     /locus_tag="ACG8H7_001126"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFP8983235.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8H7_001127"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG8H7_001127"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP8983236.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACG8H7_001128"
     CDS             complement(11562..12653)
                     /locus_tag="ACG8H7_001128"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFP8983237.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8H7_001129"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG8H7_001129"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP8983238.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8H7_001130"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG8H7_001130"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP8983239.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8H7_001131"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG8H7_001131"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP8983240.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ACG8H7_001132"
     CDS             complement(16616..17590)
                     /locus_tag="ACG8H7_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFP8983241.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ACG8H7_001133"
     CDS             complement(17571..19454)
                     /locus_tag="ACG8H7_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFP8983242.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ACG8H7_001134"
     CDS             complement(19472..21697)
                     /locus_tag="ACG8H7_001134"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP8983243.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
BASE COUNT         6198 a         4790 c         3859 g         6890 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttt
//
LOCUS       DAZKAS010000035        21069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000035 DAZKAS010000000
VERSION     DAZKAS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.035"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1213
                     /locus_tag="ACG8H7_001135"
     CDS             47..1213
                     /locus_tag="ACG8H7_001135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8983244.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            1443..2090
                     /locus_tag="ACG8H7_001136"
     CDS             1443..2090
                     /locus_tag="ACG8H7_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP8983245.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2174..3118
                     /locus_tag="ACG8H7_001137"
     CDS             2174..3118
                     /locus_tag="ACG8H7_001137"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFP8983246.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            3253..4704
                     /locus_tag="ACG8H7_001138"
     CDS             3253..4704
                     /locus_tag="ACG8H7_001138"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFP8983247.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            4725..5561
                     /locus_tag="ACG8H7_001139"
     CDS             4725..5561
                     /locus_tag="ACG8H7_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP8983248.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            5561..6220
                     /locus_tag="ACG8H7_001140"
     CDS             5561..6220
                     /locus_tag="ACG8H7_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP8983249.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            6346..7035
                     /locus_tag="ACG8H7_001141"
     CDS             6346..7035
                     /locus_tag="ACG8H7_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983250.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            7041..7997
                     /locus_tag="ACG8H7_001142"
     CDS             7041..7997
                     /locus_tag="ACG8H7_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFP8983251.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            8041..8553
                     /locus_tag="ACG8H7_001143"
     CDS             8041..8553
                     /locus_tag="ACG8H7_001143"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8983252.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(8809..9357)
                     /locus_tag="ACG8H7_001144"
     CDS             complement(8809..9357)
                     /locus_tag="ACG8H7_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP8983253.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(9446..10627)
                     /locus_tag="ACG8H7_001145"
     CDS             complement(9446..10627)
                     /locus_tag="ACG8H7_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFP8983254.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(10863..12371)
                     /locus_tag="ACG8H7_001146"
     CDS             complement(10863..12371)
                     /locus_tag="ACG8H7_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFP8983255.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            12530..12721
                     /locus_tag="ACG8H7_001147"
     CDS             12530..12721
                     /locus_tag="ACG8H7_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983256.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            12924..13841
                     /locus_tag="ACG8H7_001148"
     CDS             12924..13841
                     /locus_tag="ACG8H7_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFP8983257.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            13834..14907
                     /locus_tag="ACG8H7_001149"
     CDS             13834..14907
                     /locus_tag="ACG8H7_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983258.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            15055..15573
                     /locus_tag="ACG8H7_001150"
     CDS             15055..15573
                     /locus_tag="ACG8H7_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983259.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            15554..15946
                     /locus_tag="ACG8H7_001151"
     CDS             15554..15946
                     /locus_tag="ACG8H7_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFP8983260.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            16022..16957
                     /locus_tag="ACG8H7_001152"
     CDS             16022..16957
                     /locus_tag="ACG8H7_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP8983261.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            17090..17869
                     /locus_tag="ACG8H7_001153"
     CDS             17090..17869
                     /locus_tag="ACG8H7_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP8983262.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            17862..18179
                     /locus_tag="ACG8H7_001154"
     CDS             17862..18179
                     /locus_tag="ACG8H7_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP8983263.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            18423..20033
                     /locus_tag="ACG8H7_001155"
     CDS             18423..20033
                     /locus_tag="ACG8H7_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP8983264.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            20190..21038
                     /locus_tag="ACG8H7_001156"
     CDS             20190..21038
                     /locus_tag="ACG8H7_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP8983265.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6851 a         3746 c         4216 g         6256 t
ORIGIN      
        1 tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac
       61 tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga
      121 cggtacaacg aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca
      181 tgaagaaatc ccaatgaaag agattacaag taacgcatct ttggcactag gagaagccta
      241 tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca
      301 gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa
      361 acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt
      421 ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca atacacctga
      481 agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat
      541 caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc
      601 taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt
      661 gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga
      721 ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca
      781 tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa
      841 aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat
      901 caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat
      961 tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac
     1021 attagaacat tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg
     1081 tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc
     1141 aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat
     1201 gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt
     1261 ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag
     1321 tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag
     1381 aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat
     1441 caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc
     1501 aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt
     1561 cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt
     1621 ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt
     1681 tccaagataa atacccagat actgcaatta ctagtttgga attagatagt gattggggat
     1741 cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg
     1801 ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg
     1861 gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag
     1921 caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag
     1981 atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca
     2041 agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc
     2101 ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga
     2161 aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt
     2221 gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc
     2281 agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga
     2341 accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg
     2401 tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca
     2461 accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa
     2521 aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc
     2581 tggaaaacca caactagctt acgattttga ggccacttat gaaacactga aagaagtatt
     2641 cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt
     2701 tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag
     2761 tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga
     2821 agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc
     2881 tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg
     2941 acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtaccgagct
     3001 tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc
     3061 agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca
     3121 aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat
     3181 tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa
     3241 aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt
     3301 agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg
     3361 aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag
     3421 tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa
     3481 aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca
     3541 tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg
     3601 ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct
     3661 tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca
     3721 ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg
     3781 ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct
     3841 tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg
     3901 gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca
     3961 gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca
     4021 ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc
     4081 cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg
     4141 atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa
     4201 acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc
     4261 caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa
     4321 gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact
     4381 gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg
     4441 gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag
     4501 gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc
     4561 agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc
     4621 agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc
     4681 catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat
     4741 tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta
     4801 atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt
     4861 ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg
     4921 tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca
     4981 acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt
     5041 ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat
     5101 ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta
     5161 aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg
     5221 gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa
     5281 atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt
     5341 taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt
     5401 ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga
     5461 tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag
     5521 tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ccctaattga
     5581 tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg
     5641 gattggcttc gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga cagttttagg
     5701 tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg
     5761 cattcccgca ctagtcctgc tgtttctgtt atatttcggg ttaccttatc aattaagtgc
     5821 cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta
     5881 tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt
     5941 ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc
     6001 ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac
     6061 agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc
     6121 tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca
     6181 acagtattta gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa tggtgctagc
     6241 gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca
     6301 tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta
     6361 accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta
     6421 gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa
     6481 tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc
     6541 tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta
     6601 tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac
     6661 acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag
     6721 aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg
     6781 gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaaa agatcaattg
     6841 tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat
     6901 gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc
     6961 gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca
     7021 gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat
     7081 tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg
     7141 aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc
     7201 atccaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg
     7261 tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag
     7321 aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact
     7381 acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac
     7441 cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt
     7501 ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg
     7561 actatctttt tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta
     7621 gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg
     7681 atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag
     7741 accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc
     7801 atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa
     7861 aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta
     7921 gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat
     7981 atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg
     8041 atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc
     8101 gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca
     8161 aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat
     8221 caaattgctg gaacagctgc attagtctca ggcgtggatc ccgtttacac aaaaatagat
     8281 ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa
     8341 agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc
     8401 agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag
     8461 accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc
     8521 gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa
     8581 gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg
     8641 ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt
     8701 agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag
     8761 cttttgtcac gatctctcgg cttatcctac gagtattatt cgatacattt atatcaataa
     8821 tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag
     8881 tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct
     8941 ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt
     9001 agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa
     9061 accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc
     9121 tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt
     9181 gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc
     9241 aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc
     9301 tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt
     9361 catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt
     9421 aaaagtaact ttgctttttt gtattttata ccagcaagcc agtcacaata gcacttaaaa
     9481 agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt
     9541 tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa
     9601 atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat
     9661 ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac
     9721 cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt
     9781 aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca ttgatcatgg
     9841 caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca tggaaaccat
     9901 caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct tcaatgatca
     9961 gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga atataaccgc
    10021 caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt gccatataag
    10081 atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg aacaaacgtt
    10141 tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat tgtcctaaaa
    10201 tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt ttacttaatc
    10261 ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga ataccgataa
    10321 tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca cttcctttat
    10381 ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga agatgatcaa
    10441 acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat aatacgaaac
    10501 caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt gcttttttgt
    10561 tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata cctaataaat
    10621 aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa
    10681 tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt
    10741 agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt aaagtctata
    10801 aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt
    10861 ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct
    10921 agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct
    10981 ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg
    11041 attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc
    11101 tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct
    11161 gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat
    11221 gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa
    11281 ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc
    11341 acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact
    11401 gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg
    11461 atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga
    11521 ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata
    11581 atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg
    11641 taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct
    11701 tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac
    11761 ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt caagcaagta
    11821 gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca
    11881 atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag
    11941 tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag
    12001 actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat taataagcca
    12061 taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat cccataatca
    12121 ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga
    12181 tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt
    12241 ttcgcatgct tgtcgtcaac taacttccca ccaaattcta aatataattt gtcatagtgg
    12301 ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca
    12361 atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt
    12421 cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt
    12481 ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag
    12541 acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag
    12601 aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca
    12661 gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata
    12721 aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga
    12781 tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct
    12841 tttttataac aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag
    12901 atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag
    12961 acgaactaga ggagacgaac gcggatcagg atacaggaca agaatcgtct tctttttacg
    13021 atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg
    13081 aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga
    13141 atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta
    13201 agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc
    13261 caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg
    13321 atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt
    13381 cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca
    13441 ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga
    13501 ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag
    13561 aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg
    13621 agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc
    13681 agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca
    13741 aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc
    13801 ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg
    13861 acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa
    13921 acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacat
    13981 gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa
    14041 ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt
    14101 ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa
    14161 tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc
    14221 tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg
    14281 aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt
    14341 tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc
    14401 atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg
    14461 gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga
    14521 gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat
    14581 gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt
    14641 ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg
    14701 gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga
    14761 ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg
    14821 atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg
    14881 gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga
    14941 taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg
    15001 tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc
    15061 acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct
    15121 tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac
    15181 ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat
    15241 tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg
    15301 ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt
    15361 ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat
    15421 cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt
    15481 ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat
    15541 tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact
    15601 cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt
    15661 aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa
    15721 agaagtggtc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt
    15781 caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca
    15841 actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa
    15901 attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac
    15961 tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa
    16021 catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat
    16081 tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa
    16141 cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa
    16201 acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt
    16261 tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga
    16321 agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc
    16381 aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat
    16441 caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag
    16501 tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt
    16561 agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt
    16621 gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct
    16681 gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg
    16741 tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt
    16801 agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa
    16861 tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga
    16921 aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc
    16981 aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat
    17041 gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa
    17101 gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat
    17161 accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc
    17221 aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct
    17281 tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta
    17341 tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc
    17401 tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac
    17461 tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg
    17521 ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct
    17581 tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc
    17641 actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt
    17701 ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct
    17761 ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa
    17821 tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc
    17881 tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt
    17941 attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg
    18001 cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg
    18061 cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt
    18121 cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa
    18181 ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga
    18241 actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag
    18301 tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct
    18361 atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt
    18421 aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta
    18481 aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg
    18541 gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc
    18601 ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag
    18661 gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg
    18721 gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga
    18781 ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta
    18841 tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg
    18901 gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt
    18961 acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt
    19021 ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact
    19081 tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg
    19141 cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta
    19201 tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga
    19261 tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga
    19321 cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca
    19381 cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg
    19441 cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca
    19501 cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt
    19561 ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc
    19621 gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg
    19681 gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg
    19741 tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga
    19801 tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg
    19861 ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt
    19921 attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa
    19981 atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact
    20041 aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc
    20101 tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa
    20161 aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg
    20221 catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga
    20281 ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac
    20341 ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg
    20401 gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt
    20461 cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa
    20521 cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg
    20581 ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa
    20641 tccgagataa tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag
    20701 gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga
    20761 cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg
    20821 ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt
    20881 acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa
    20941 aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag
    21001 gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata
    21061 ccaaaaata
//
LOCUS       DAZKAS010000036        20185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000036 DAZKAS010000000
VERSION     DAZKAS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.036"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..1509
                     /locus_tag="ACG8H7_001157"
     CDS             106..1509
                     /locus_tag="ACG8H7_001157"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP8983266.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            1509..2942
                     /locus_tag="ACG8H7_001158"
     CDS             1509..2942
                     /locus_tag="ACG8H7_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP8983267.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            3087..4013
                     /gene="trxB"
                     /locus_tag="ACG8H7_001159"
     CDS             3087..4013
                     /gene="trxB"
                     /locus_tag="ACG8H7_001159"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP8983268.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            4227..5954
                     /locus_tag="ACG8H7_001160"
     CDS             4227..5954
                     /locus_tag="ACG8H7_001160"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFP8983269.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            5969..6319
                     /locus_tag="ACG8H7_001161"
     CDS             5969..6319
                     /locus_tag="ACG8H7_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP8983270.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(6411..7271)
                     /locus_tag="ACG8H7_001162"
     CDS             complement(6411..7271)
                     /locus_tag="ACG8H7_001162"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFP8983271.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            7363..7791
                     /locus_tag="ACG8H7_001163"
     CDS             7363..7791
                     /locus_tag="ACG8H7_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP8983272.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            7953..8129
                     /gene="rpsU"
                     /locus_tag="ACG8H7_001164"
     CDS             7953..8129
                     /gene="rpsU"
                     /locus_tag="ACG8H7_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP8983273.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            8156..8602
                     /locus_tag="ACG8H7_001165"
     CDS             8156..8602
                     /locus_tag="ACG8H7_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFP8983274.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(8756..10135)
                     /gene="brnQ"
                     /locus_tag="ACG8H7_001166"
     CDS             complement(8756..10135)
                     /gene="brnQ"
                     /locus_tag="ACG8H7_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP8983275.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            10841..11812
                     /locus_tag="ACG8H7_001167"
     CDS             10841..11812
                     /locus_tag="ACG8H7_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP8983276.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            11836..14028
                     /locus_tag="ACG8H7_001168"
     CDS             11836..14028
                     /locus_tag="ACG8H7_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFP8983277.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            14045..14521
                     /gene="ybeY"
                     /locus_tag="ACG8H7_001169"
     CDS             14045..14521
                     /gene="ybeY"
                     /locus_tag="ACG8H7_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP8983278.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            14505..14900
                     /locus_tag="ACG8H7_001170"
     CDS             14505..14900
                     /locus_tag="ACG8H7_001170"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFP8983279.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            14915..15814
                     /gene="era"
                     /locus_tag="ACG8H7_001171"
     CDS             14915..15814
                     /gene="era"
                     /locus_tag="ACG8H7_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP8983280.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            15957..16769
                     /gene="recO"
                     /locus_tag="ACG8H7_001172"
     CDS             15957..16769
                     /gene="recO"
                     /locus_tag="ACG8H7_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP8983281.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            17153..18070
                     /gene="glyQ"
                     /locus_tag="ACG8H7_001173"
     CDS             17153..18070
                     /gene="glyQ"
                     /locus_tag="ACG8H7_001173"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP8983282.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            18072..20147
                     /gene="glyS"
                     /locus_tag="ACG8H7_001174"
     CDS             18072..20147
                     /gene="glyS"
                     /locus_tag="ACG8H7_001174"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP8983283.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         6769 a         3575 c         4382 g         5459 t
ORIGIN      
        1 aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt ttgttttgtt
       61 tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc aaaaggaatc
      121 aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga aggatttatc
      181 aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga agaaggaaaa
      241 gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc tccgtatgaa
      301 gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga ttttgttacg
      361 actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat ccctagttat
      421 tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc ttttcgtacc
      481 gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa tgcttggctg
      541 atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata tggcaaatgg
      601 gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga accagcaatg
      661 cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca tttccattgg
      721 cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga tcgtttatac
      781 gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa agaacaatta
      841 aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga agaagaaatg
      901 ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca agtgaaagaa
      961 acagaagcag agctatttga attatacaaa gatccagaat tagaccataa acctgaacaa
     1021 ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat tgcttcaatc
     1081 tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc tgttcctgac
     1141 ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg agcgagaaat
     1201 gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat gaagaatatg
     1261 gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt acaggcgttt
     1321 acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga tgagttgttt
     1381 attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt aaaagaagaa
     1441 ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca tataatggag
     1501 gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca gccaatcaat
     1561 gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt gtgccaatcg
     1621 gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt gatgaggatc
     1681 atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa gatattgctt
     1741 tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt cgtatttttc
     1801 caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag gatgccttta
     1861 aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt gattgtccaa
     1921 tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt ttttatgaaa
     1981 atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg ctgacattca
     2041 atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat tttgaaaaag
     2101 gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt gcaagtgcca
     2161 tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt atgctggcag
     2221 caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct agaaaatcag
     2281 atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc gcttatgcat
     2341 tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat gccgcattgc
     2401 tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga gtttcgacta
     2461 ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc aaaaatccat
     2521 atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga ataacgatga
     2581 atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc ttaggtgcag
     2641 tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat tacctggcag
     2701 cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta ttaggctata
     2761 cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag aaaaggtacg
     2821 gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt tcaaagaaaa
     2881 aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt tcgaacgatt
     2941 gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat aataaagcta
     3001 ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa gtatgcttac
     3061 ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc gcaggtcctg
     3121 ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg atgatcgaac
     3181 aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat cctggattcg
     3241 attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca agatttggga
     3301 cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat aaagaagtca
     3361 tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga tgtatccacc
     3421 gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct tactgtgcag
     3481 tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt ggggactcag
     3541 cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc gtccaccgta
     3601 gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat gaaaaaattt
     3661 cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta gtgacaggtg
     3721 tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac ggagtattca
     3781 tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg acgaacgaag
     3841 ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt tttgccatcg
     3901 gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac ggcggtattg
     3961 ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca taagtttaaa
     4021 aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg aaatttactg
     4081 aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt cttgatttaa
     4141 gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt attaagaacc
     4201 atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa caatgggtac
     4261 atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt gaagatcctg
     4321 aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt atgcggggaa
     4381 tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca acagaaggac
     4441 tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc gctatcgctt
     4501 atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca ctagcacatc
     4561 acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt tcttttgcag
     4621 ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac ccagcggctt
     4681 acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca gacgcagatg
     4741 cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta ttatctgacg
     4801 aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac gctgcttatc
     4861 tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg gggaaagatt
     4921 tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa cgtgcgttga
     4981 aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca gatccagatt
     5041 tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat gctatacgac
     5101 ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca gaccgtttag
     5161 gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac caaatcggag
     5221 cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta ccagaaaacg
     5281 cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg gaacactacg
     5341 gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa atccaacaat
     5401 ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat ggttatttag
     5461 ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta gcagaagtag
     5521 cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg atttttgaaa
     5581 cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa gaaggttctg
     5641 cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa tttgccggag
     5701 caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt gaaggaaacg
     5761 tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa gaaaatggct
     5821 ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt ggtgtaaaag
     5881 gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt atcaaaaaga
     5941 tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc
     6001 ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag tagagaaagt
     6061 cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc tatatctgaa
     6121 agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc agtctgctgt
     6181 atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc gcgagggaaa
     6241 agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc agacactcaa
     6301 acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg tgacaacgaa
     6361 gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga ttaatcgctt
     6421 ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc attcaaaata
     6481 agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca tcctagtttt
     6541 tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc ggaataagca
     6601 gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt taaaatatct
     6661 gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg tttgggaata
     6721 tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc taaaaacaag
     6781 ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc ttctaaaaaa
     6841 ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt gatccgtttg
     6901 aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt gataccagtt
     6961 aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa ttgatgttct
     7021 tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg caggacaagt
     7081 gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc tgtcacgaac
     7141 gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc ttgcgctagt
     7201 tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat ttctgtagaa
     7261 tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata ccatactcta
     7321 gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa acagacattg
     7381 atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa aaaaagagaa
     7441 gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga agtttttgac
     7501 tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa tctccatgat
     7561 tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa gtttcgtttt
     7621 cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt gtgcggaaca
     7681 acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg tggatgcacg
     7741 atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata aggatttaaa
     7801 taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc aaaacagcat
     7861 tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata acaattacgg
     7921 ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt cgcaaaaacg
     7981 aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg ggtactttac
     8041 aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag aaaaaatctg
     8101 aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg gtagtgtgtc
     8161 actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag ataaggaatc
     8221 tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta aagaaggtca
     8281 cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac agcgacgtga
     8341 ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag taaaaagtga
     8401 aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa ttcgacaact
     8461 tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg gcaaagttat
     8521 gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga atgcgattgt
     8581 gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat tgtccgcagt
     8641 tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa gattctttct
     8701 tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga tttggttatt
     8761 cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg taataaaaga
     8821 gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct aatgcagcaa
     8881 aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc tgactcacta
     8941 atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag aacatcgtta
     9001 cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc aagattaacg
     9061 caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg atattatcca
     9121 atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat gtcatataac
     9181 tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt cccatagcgg
     9241 tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag ttacctaaat
     9301 aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc atcttattca
     9361 aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac gcaacgctca
     9421 ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca agatcacgaa
     9481 ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc gtgttgtatc
     9541 cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca gattggttta
     9601 gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat gcaagaaaca
     9661 aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca gtaaggaaat
     9721 aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca tatttttcag
     9781 gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt gtaccaaaga
     9841 atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat tttccgacag
     9901 gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag atagctaata
     9961 atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag tttgatccag
    10021 ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac atcccgaaga
    10081 taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct tccataaatt
    10141 tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac tagaaatgcc
    10201 actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc ccttttccac
    10261 ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa aaaagcttct
    10321 tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag gcagccacaa
    10381 tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt tattgtcatt
    10441 tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt tcctcgacgc
    10501 tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa atgcaagaat
    10561 atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga atacttgcta
    10621 agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag ctttttttat
    10681 tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg gtacttatgg
    10741 ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg agtatggtaa
    10801 aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag agtctagttc
    10861 tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg cacacgacaa
    10921 gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg gagaaatcat
    10981 tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc ggacattgca
    11041 ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg cactgaaaat
    11101 ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga tcgtcaaaga
    11161 ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg tcgaatcggt
    11221 agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa cttttcttgc
    11281 agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca ttttgacgcg
    11341 gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa aagaaaaagt
    11401 agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat tagatcatac
    11461 gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca tgcgggggcg
    11521 gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag ttgctcagat
    11581 gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg gagatacgag
    11641 tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac gaacattaaa
    11701 gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc ggcaccctgt
    11761 agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat aagctttgct
    11821 taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa acttggaaag
    11881 gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt tacatttagt
    11941 agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga aagtatccgt
    12001 gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact ggcagcagaa
    12061 gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa caatcggatc
    12121 aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc ttatgacgaa
    12181 aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga agagcggatc
    12241 gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt ttatcaaaaa
    12301 ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat ccatactgtg
    12361 agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg tgaaagtgaa
    12421 ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt cacaccagca
    12481 caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga cgtattgaat
    12541 gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc agtgatgatc
    12601 ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc tatgaaaaaa
    12661 ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat tgccatggtt
    12721 ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt ccaaaatgca
    12781 ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag catagtgctg
    12841 atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct gttttatccc
    12901 gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg gatcatggct
    12961 ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg cacgaacttt
    13021 ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat tgtcagacgc
    13081 aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt tcctgtgatg
    13141 atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac gacttggtta
    13201 atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat cggtctacac
    13261 ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga actcagcaat
    13321 ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta tcatcatagt
    13381 atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg ttctttgcta
    13441 actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag ctttttcgtt
    13501 gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga agacagtaaa
    13561 catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta taatatgccg
    13621 caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg ttatttctac
    13681 atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta tcctggacca
    13741 aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga ggctgcagta
    13801 cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa tcttatcgaa
    13861 gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga aatccgaaaa
    13921 gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat caaatatcct
    13981 aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa agaagcgatg
    14041 tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga aaatgaaaga
    14101 aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg aaacagaaat
    14161 gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt accgtggaaa
    14221 agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag acgaaatccc
    14281 agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca tcatgatttc
    14341 tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc gggaacttgg
    14401 ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga caccagaaga
    14461 tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac tcaaaagata
    14521 aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc caaggagtca
    14581 aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt atccttgctt
    14641 tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg cttttagctg
    14701 tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta gtggacatgt
    14761 ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca gctggagcag
    14821 ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc cctaaaatat
    14881 atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat cagggtttgt
    14941 cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa tcgtgggtca
    15001 aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc aaggagtata
    15061 tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata aaccaaagca
    15121 tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag tggatgcagt
    15181 cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta ttattgaacg
    15241 tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata agatccatcc
    15301 agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg cacaagtgat
    15361 tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat tagtcagtga
    15421 aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc ctgaatactt
    15481 tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg aagtgcctca
    15541 ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa tccatatcca
    15601 ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca aaggcggaaa
    15661 aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt taggagacaa
    15721 agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa aagtctatct
    15781 gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta taggttaagt
    15841 atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga tcgaaacata
    15901 aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg gagaatatga
    15961 gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa gaaaaagata
    16021 aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc aaaggaatcc
    16081 atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca gtctatatcg
    16141 gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc catgcgtttc
    16201 ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta aatctagtag
    16261 atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg caacaagctc
    16321 tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt gaagttcaaa
    16381 tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc ggggaaacaa
    16441 aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa cactggccaa
    16501 tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga atgttttcgg
    16561 ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag caggcaatcc
    16621 gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa agcaaaaagt
    16681 tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa aatatgcctg
    16741 aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa tcaaaactat
    16801 ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat cgttcactga
    16861 gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga ccaagatcag
    16921 taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa cgatttttga
    16981 agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa gtagggtgga
    17041 accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt gtcgttttct
    17101 ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac agatgagtaa
    17161 aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat caaacggctg
    17221 catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc catatacatt
    17281 cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat cccgccgacc
    17341 agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc aatttcaagt
    17401 cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct tgcgtctttt
    17461 aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg agaacccatc
    17521 gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga tcacacaatt
    17581 cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg agatcactta
    17641 tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg atctggaatg
    17701 ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac attccaaata
    17761 ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt ttgaaaaaga
    17821 agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata ttttgaaatg
    17881 cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag aacgagcagg
    17941 ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg tggcagaacg
    18001 cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa acatgaagga
    18061 ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa atgcctgctc
    18121 atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt ttagatgaaa
    18181 accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta gcaatcaaag
    18241 taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga ccagccaaaa
    18301 aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt gtccgcggac
    18361 aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa tatgtctatg
    18421 taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta gcagatgtca
    18481 ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt gaatatatcc
    18541 gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt gagatcttag
    18601 atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat gtgacatttg
    18661 cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct tcaccgcaaa
    18721 aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat cactgggtta
    18781 ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat ccaactgtct
    18841 tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta gtcacttcta
    18901 tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg cttcctcatt
    18961 ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga ggcaatcaaa
    19021 aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac aagaaagctt
    19081 caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa atcggtagta
    19141 tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa caagtaggac
    19201 tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa tttgatctag
    19261 taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa aaatatgctc
    19321 tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac atgcctatat
    19381 ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt gcagataaat
    19441 tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca aacgatcctt
    19501 atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa aaatgggcct
    19561 ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat acggatcgtt
    19621 tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa ggccgtctgc
    19681 gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt ttgaatgcag
    19741 aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag catcttgcag
    19801 atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta gcgaaaaaag
    19861 cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa gcggagaaag
    19921 aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg atcaacgaaa
    19981 attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat cagacgatgg
    20041 tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt caaattgctg
    20101 ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt ctaatatgta
    20161 aataaaccag agaataggca caaaa
//
LOCUS       DAZKAS010000037        19882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000037 DAZKAS010000000
VERSION     DAZKAS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.037"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACG8H7_001175"
     CDS             202..1077
                     /locus_tag="ACG8H7_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP8983284.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACG8H7_001176"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACG8H7_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACG8H7_001177"
     CDS             complement(1642..2973)
                     /locus_tag="ACG8H7_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8983285.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ACG8H7_001178"
     CDS             complement(3122..3835)
                     /locus_tag="ACG8H7_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8983286.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ACG8H7_001179"
     CDS             4010..5434
                     /locus_tag="ACG8H7_001179"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8983287.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ACG8H7_001180"
     CDS             5733..6440
                     /locus_tag="ACG8H7_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFP8983288.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ACG8H7_001181"
     CDS             6427..6750
                     /locus_tag="ACG8H7_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFP8983289.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ACG8H7_001182"
     CDS             complement(6839..7876)
                     /locus_tag="ACG8H7_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP8983290.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ACG8H7_001183"
     CDS             complement(8197..9180)
                     /locus_tag="ACG8H7_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFP8983291.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ACG8H7_001184"
     CDS             complement(9595..10365)
                     /locus_tag="ACG8H7_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983292.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ACG8H7_001185"
     CDS             complement(10358..11293)
                     /locus_tag="ACG8H7_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983293.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ACG8H7_001186"
     CDS             complement(11609..13258)
                     /locus_tag="ACG8H7_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP8983294.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ACG8H7_001187"
     CDS             complement(13375..15978)
                     /locus_tag="ACG8H7_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP8983295.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ACG8H7_001188"
     CDS             complement(15990..17039)
                     /locus_tag="ACG8H7_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP8983296.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ACG8H7_001189"
     CDS             complement(17065..17379)
                     /locus_tag="ACG8H7_001189"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP8983297.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ACG8H7_001190"
     CDS             complement(17395..17841)
                     /locus_tag="ACG8H7_001190"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP8983298.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ACG8H7_001191"
     CDS             complement(17842..19764)
                     /locus_tag="ACG8H7_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8983299.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         6081 a         3967 c         3230 g         6604 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aa
//
LOCUS       DAZKAS010000038        19494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000038 DAZKAS010000000
VERSION     DAZKAS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.038"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..214
                     /locus_tag="ACG8H7_001192"
     CDS             <1..214
                     /locus_tag="ACG8H7_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP8983300.1"
                     /translation="WKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEK
                     YKEILEEYLRRTANDKKTRSIQHGFI"
     gene            174..419
                     /locus_tag="ACG8H7_001193"
     CDS             174..419
                     /locus_tag="ACG8H7_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983301.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            416..835
                     /locus_tag="ACG8H7_001194"
     CDS             416..835
                     /locus_tag="ACG8H7_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP8983302.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            832..1389
                     /locus_tag="ACG8H7_001195"
     CDS             832..1389
                     /locus_tag="ACG8H7_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP8983303.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1386..1682
                     /locus_tag="ACG8H7_001196"
     CDS             1386..1682
                     /locus_tag="ACG8H7_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983304.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1759..2172
                     /locus_tag="ACG8H7_001197"
     CDS             1759..2172
                     /locus_tag="ACG8H7_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP8983305.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            2518..2892
                     /locus_tag="ACG8H7_001198"
     CDS             2518..2892
                     /locus_tag="ACG8H7_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983306.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            2929..3096
                     /locus_tag="ACG8H7_001199"
     CDS             2929..3096
                     /locus_tag="ACG8H7_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983307.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            3122..3466
                     /locus_tag="ACG8H7_001200"
     CDS             3122..3466
                     /locus_tag="ACG8H7_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP8983308.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            3471..3752
                     /locus_tag="ACG8H7_001201"
     CDS             3471..3752
                     /locus_tag="ACG8H7_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983309.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3855..4169
                     /locus_tag="ACG8H7_001202"
     CDS             3855..4169
                     /locus_tag="ACG8H7_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFP8983310.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            4147..5841
                     /locus_tag="ACG8H7_001203"
     CDS             4147..5841
                     /locus_tag="ACG8H7_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP8983311.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            5861..7039
                     /locus_tag="ACG8H7_001204"
     CDS             5861..7039
                     /locus_tag="ACG8H7_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP8983312.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            7002..7688
                     /locus_tag="ACG8H7_001205"
     CDS             7002..7688
                     /locus_tag="ACG8H7_001205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFP8983313.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            7688..8848
                     /locus_tag="ACG8H7_001206"
     CDS             7688..8848
                     /locus_tag="ACG8H7_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP8983314.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            8858..9733
                     /locus_tag="ACG8H7_001207"
     CDS             8858..9733
                     /locus_tag="ACG8H7_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983315.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            9730..10041
                     /locus_tag="ACG8H7_001208"
     CDS             9730..10041
                     /locus_tag="ACG8H7_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983316.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            10031..10384
                     /locus_tag="ACG8H7_001209"
     CDS             10031..10384
                     /locus_tag="ACG8H7_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP8983317.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            10374..10775
                     /locus_tag="ACG8H7_001210"
     CDS             10374..10775
                     /locus_tag="ACG8H7_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983318.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            10768..11172
                     /locus_tag="ACG8H7_001211"
     CDS             10768..11172
                     /locus_tag="ACG8H7_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983319.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            11184..11792
                     /locus_tag="ACG8H7_001212"
     CDS             11184..11792
                     /locus_tag="ACG8H7_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP8983320.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            11812..12174
                     /locus_tag="ACG8H7_001213"
     CDS             11812..12174
                     /locus_tag="ACG8H7_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983321.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            12177..12359
                     /locus_tag="ACG8H7_001214"
     CDS             12177..12359
                     /locus_tag="ACG8H7_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983322.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            12376..15795
                     /locus_tag="ACG8H7_001215"
     CDS             12376..15795
                     /locus_tag="ACG8H7_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP8983323.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            15846..16583
                     /locus_tag="ACG8H7_001216"
     CDS             15846..16583
                     /locus_tag="ACG8H7_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP8983324.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            16593..18884
                     /locus_tag="ACG8H7_001217"
     CDS             16593..18884
                     /locus_tag="ACG8H7_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFP8983325.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            18908..>19494
                     /locus_tag="ACG8H7_001218"
     CDS             18908..>19494
                     /locus_tag="ACG8H7_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFP8983326.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         6958 a         2843 c         4145 g         5548 t
ORIGIN      
        1 ttggaaagca gcagcaaaca ttgaatttta ttacaacgaa attcaaaaag ggttcgatca
       61 gatgcgtgag ctggatcggc aaacaaattg gagcaagaaa cttcatcaag atcgtttcaa
      121 atttgtagag aagtataaag aaatattaga agagtatttg aggaggacag caaatgataa
      181 aaaaactcgt tcaattcagc atggatttat atgatatcga atcaggagca acactatctg
      241 tggaatcgga ccatctaatc ataaattttg gtggaaagcg ccagattatt ttgtgggtag
      301 ttgatgatgt actgtttcca gaaattgttc atgatttcga agaatcaaaa gcggttgagt
      361 ttgaaatagt gaaaaaagta atggaattga ttgaaaaata cgaggaggac agcgaatgat
      421 accgaagttt agagcgtggg ataaacgaaa gaacgtaatg agagatgtag ccgtcttgca
      481 ttttactaaa aacggcaaaa caaactttat tgaatattgg ataaatccta ccgaattgaa
      541 atcatatcac gtgcgaaaca tcgacctcat gcaatccaca ggaatgaaag ataagaatgg
      601 tgtggatata tttgaagggg atatagtatt agtcagcgtg cgaaatggct tcgattactt
      661 agataataaa gtctgtattg tcaaaaattc aatagattat tccggattag tttgtgccac
      721 tgttgatgaa gacttagagt atcaaatttt taacacagag ctgtttgaag aatacacgta
      781 cgaagtcacc ggaaatatat acgagaatag cgagttattg gaggaacagc gatgaataaa
      841 caggaagcaa tcaaaaagtt agaaagtatt aaagcgatag gaaatgatgc aatagctgct
      901 tgctataacg agagtataaa ttcaggtatt acgttaatga aaaaaataga cgaaccgcag
      961 aaaccagtta ttccacaact cgtggccggt tggcttgaga aatctacgga cccttttaca
     1021 aaagctgaaa aaatagcgta tttaatcaaa tctaaagatg gtgattcata ttatttctgt
     1081 gattggtttg tacgagatgg catagtgacg caagagcaag gagaagaatt actcgcttgg
     1141 gcaacaagac aatcatatga aacactattg agcctataca acggctacga ggttgagaaa
     1201 gagccgttat gggcaataaa gaatgccgat ggaaactatc ttactaaatg tgctttatgg
     1261 ggaaaagatg gagtaaatta tagttttgaa tgcaatccat ctcatcgatt gcttttcact
     1321 gataaagcaa cagcggatgc tgcagcattg ttggtgaatg gaacagtgga agaggtggta
     1381 gaaagatgaa actaaaagac ggattttacg ctagcagtca cggtatcggc ggtttaatgc
     1441 tagatatgcc gacaaagaac cctaaaacac gcaagaaatc aaaattcaaa gtcggtgaca
     1501 tggtccgctg tgaagcagaa gagttcgttt atccgttcag aggatacgtt aaaaagatac
     1561 tgtcaaactc agcaatcatt cgtattgaaa atacaatgaa gtgtgatcgt tcgactgcaa
     1621 aacacaagca ttatttagct gtagcgagat tagttgacat ggaagtaatc aagagcaaat
     1681 aaaaaaagcc ggatcgctcc gactgattca ataaatccaa cacatttatt atatcacata
     1741 aaggagcggt ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt
     1801 aatgcgagag atgtgctgaa gaactttcgg cgtttggagc ggatggcagg tcgctctttg
     1861 atagatatta agtcgccgat tattacggat atgccgaagg caccaaagca cggcaataag
     1921 gcagaagacg cgatcattca gatgatggat atagaagcgg agagagatgc gattctagcg
     1981 gctttgatgg ctcttagtct aattagtcgc cagatactct actacagctt ctgtgtgcca
     2041 gatagcttct caaactacag gattagccgt gaagtgggtt attcagaaag aagtatacaa
     2101 cggatgaagt cggaagctct aatagagttt gcagaagcat ataaacacgg aagaataatt
     2161 gcttataaat aatttggcgg ttttttggcg gaatgatggc ggtttttagc tatttaccag
     2221 tgatattatg gtagtgtcga aagattagtg ataggtctaa gacaaaataa aatgtaaggg
     2281 aggaaatctc cctcatcgtt taattaagct tcgatagaca gcagcaaata aactaaagga
     2341 tgtgggggtc agctcctaca gttagttcat gtgttgctgt ctattaattg caactctttc
     2401 ggttttattg agtatttact caacttaaaa aaactgttgt ttgtagatgt aagctgttcc
     2461 tatttgtaca tagtaaaaaa tgatataata aaaatattac gaaaggggtg agagtgcatg
     2521 gaagacaaaa tgtatttaag acaaaaagtt ttcacaaaaa aagatattga aaatcaaatt
     2581 aatggattta tacagaaatt agaaaatgca aattcttatt tgataaataa ggaactaaat
     2641 aaagcttatg accagtggaa agaagtatat gatgagttga aattgattca aaatgaaaca
     2701 aaattagtac gaacggagaa aaagaatgaa aatagtttct tttttgatgg atatgctata
     2761 ttgatgttgg aaactgtagc aaaacaaaat ataaaagctc ccaaaaaaga actatctgac
     2821 aatattgata acgcattagc cgagttaagg tattacgtta tgcaaataaa agatgttagg
     2881 atcactcgtt gagtggtctt tttattttgc acaaaggagg taacaacaat gtatagacca
     2941 caatacttag aacagaagta tgaagtaatc actgtgcaaa atggtaacgg agagatagta
     3001 cgaaagtata gaagaccaat aaagagcgat acatataaac gaaaggaaag caatgaagtt
     3061 attccattgt atggcaaaag aatagctaag cattaaataa gattgtgaaa ggagacggga
     3121 catgaacgag gaattctata gatggctatt acagttgata agagaagatc gtttggttaa
     3181 gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac gagatcacta
     3241 tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg ttcatcatat
     3301 aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta tttgtttatg
     3361 tgttgaacat cataatgaag ttcatggcag atatcttaca gcattggata aacaagagaa
     3421 gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc atgatcatca
     3481 atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct tatactcagg
     3541 gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg ttatcagata
     3601 actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa gataataacg
     3661 tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat tacattgact
     3721 tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat cgcaaatttt
     3781 ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg
     3841 aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac
     3901 gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata
     3961 atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg
     4021 agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg
     4081 tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg
     4141 aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa agctatagaa
     4201 gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga
     4261 gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata
     4321 ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt
     4381 tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg
     4441 ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct
     4501 aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag
     4561 actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta
     4621 tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac
     4681 acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt
     4741 cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg taaaattcga
     4801 gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac
     4861 attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca
     4921 gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat
     4981 cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcaaattt aaaacggaaa
     5041 atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc
     5101 aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt
     5161 ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat
     5221 tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt
     5281 tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga
     5341 gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata
     5401 gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa
     5461 atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt
     5521 actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat
     5581 gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc
     5641 atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct
     5701 gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat
     5761 atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga
     5821 tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc
     5881 ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt gtaaattgca
     5941 agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc
     6001 gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta
     6061 ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact
     6121 gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag
     6181 cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt
     6241 ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat
     6301 ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta
     6361 tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt
     6421 actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat
     6481 gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg tatttcaatt
     6541 acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat
     6601 aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa
     6661 cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga
     6721 aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga
     6781 agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt
     6841 caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga
     6901 aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat
     6961 aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat
     7021 ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt
     7081 ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag
     7141 gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga
     7201 ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct
     7261 tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca
     7321 atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc
     7381 gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac
     7441 gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg
     7501 cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag
     7561 gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa
     7621 gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg
     7681 taaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa
     7741 agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga
     7801 aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat
     7861 gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa
     7921 aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt
     7981 tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg
     8041 tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga
     8101 agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct
     8161 atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg
     8221 ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga
     8281 tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg
     8341 agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa ctatgggccg
     8401 tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa
     8461 tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt
     8521 cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt
     8581 ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga
     8641 ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt
     8701 tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga
     8761 tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac
     8821 aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc
     8881 ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg
     8941 aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta ttttttttga
     9001 gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat
     9061 tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga
     9121 cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa
     9181 attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga
     9241 ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa
     9301 tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat
     9361 taaccgtcct aaagcgaatt attggaaaaa ggtttatctg gattctaatc ggctatatcg
     9421 ctaccttaga gggcaaaaaa caaatataca aatttggaca gccgagatgt gggcacggct
     9481 ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt
     9541 tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac
     9601 aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga
     9661 agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc
     9721 ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat
     9781 atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct
     9841 gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac
     9901 ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa
     9961 aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca
    10021 ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa
    10081 gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg tagaagatac
    10141 agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat
    10201 ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa
    10261 gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta
    10321 tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga
    10381 ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct
    10441 tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg
    10501 atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat
    10561 atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag
    10621 gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg
    10681 atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt
    10741 ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat
    10801 ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt
    10861 aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt
    10921 cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt
    10981 aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta
    11041 aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga
    11101 agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa
    11161 ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca acgttcgata
    11221 acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca atcgcaacga
    11281 attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag
    11341 cgacagaagt aaaatcaaaa tctaaaccaa tcaatatgac ggtaacaatt actgcacatg
    11401 taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt attaaaccag
    11461 gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct gcagagatcg
    11521 tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact tcgaatacag
    11581 gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa
    11641 aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag
    11701 aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag
    11761 ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa
    11821 gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat
    11881 atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgact
    11941 gtgggaaaag gatccatgga tatttctgtt attcaaggtg caaaggctgt gtatattgct
    12001 taccgccaag cgaacatgac tgattatatt tcattcgatg aatttatcga taaatgggat
    12061 tttgatatgg ctactgccag ctatatttat caattgatga tgttcaaaca agcacgcgat
    12121 gcttatcaaa aagaatttga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc
    12181 caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta
    12241 tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca
    12301 acaagagtgc ttatgaagga tggaaaaact atgcaataaa ccaatctcag aaaaactaaa
    12361 gaaaggaggt aaaaaatggc taaaaagaga acagaagcag aagtaacttt catagctaac
    12421 gatgacggat tgaaatctac gttaaaagaa atcagtgctg aattaactaa aaatagagca
    12481 gaattaaaac tagaacaagc tcaattacaa cagactggtt ctgaatcaga caagttagga
    12541 agtaaattat cttctttaga aaagcagtat gaattacaaa gccaaaaagt tgaagtaact
    12601 agccaacgtt tagccaatgc caaaaaatat tatggagaaa attccaccga agttcagaaa
    12661 cttgagagag aactgattaa ccaacaaaca gcgcaacaac gtttgtcaaa cgaaattgat
    12721 aaaacgagta atacactagc tcaagcaaaa ggcgaaatac agacgtacga gtctacaatg
    12781 caacagttgg atagtgaaca aaaaaatgtt caagctagtg cttctctgat tgaatccgaa
    12841 tacaaaaaat ggcaagtaac tgctggtcaa tcagcttctg aatccgagaa attagcgaaa
    12901 gcccaagaat atgtttctca acaatctgaa aatgcagaga aaacgataga tatcctgaga
    12961 cgacagttag aagctacaca gtctgagttt ggcgctacat ccacagaagc aatgcagatg
    13021 gaggcgaagc ttaatgatgc tgaacgtgaa tttgaagagt taggacaagc tgctaaaaat
    13081 gtagatacaa ctaacttgga cgatatcgga agcaaaatag atatgaataa tttaatggaa
    13141 gcttctgacg ttttaagcga cattggcgat aagcttacag aattagggaa acaagcagtg
    13201 gactctgcta acagtgtagg tagttcccag agtaaaatac aagctaattt tggtttgact
    13261 aaacaagagg ctgaagaatt aacgaatgta gccagagaca tttattataa aggttttgga
    13321 gaatcgttag atcagtccac agatgcattg attttggtaa agcgtaattt aggcgattta
    13381 aataatcaag atttacaaaa tattacggaa caagctatgg tcctagaaaa caccatgggc
    13441 gctgatatgg atgaaacgtt acgtggtgta aatggcttaa tggtcaactt cggattgagc
    13501 gctcaagatg caatggattt aatggtttcg ggtactcaaa acggtttaga taaaacgcac
    13561 gaattaggcg acaatatggc agaatatagc caattatgga gtcaaatggg atattcagct
    13621 gatgaaacgt tcggaatgct tcaaaatggt ttagatgcgg gtgcttataa ccttgataaa
    13681 gtcaatgact tagttaagga aatgggaata tcgttaacag atggtcgatt tgagcaaaac
    13741 atggatatgt ttagtgaaag tactagaaaa gcttttgaag agtggaaaaa tggtggagga
    13801 acacaaaaag acgttattaa ttccatgatt caagatttta gcaatatgga tggtcaatac
    13861 gaccaattaa ataaagcttc tacaatttgg tctgcacttg gcgaagataa cgcgatgaaa
    13921 gttgtccaat ctttgactga tgttaaccat acatttgatg atgttagtgg atctgcacaa
    13981 aaaatgaatg aagattctac tactccgtta caagaattaa acggaaaaat agctgaatta
    14041 aaggattcat tagctcctat aggcaacaca atcatagatg cactcgaacc agtaattgat
    14101 tttctaggaa agatggctga tgcgtttaat aatcttccac aaccagtaca ggattatgcc
    14161 gtagcgattg gcggattgac tgctgcattt actttattaa tgccaataat agttggcttc
    14221 atggctctag gtggtcctac tacattaata ataggagcag ttattactgc tattgctgga
    14281 gttatagcaa ttataaaaaa ctggggcgca attactgact ggtttaaggg aatatggagt
    14341 aaattcactg attggttggg tggtacttgg gaaagcataa aagaaggtgc ctcatcagtt
    14401 tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga aatttggcaa
    14461 ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc ttccaacatc
    14521 tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa tatttggaac
    14581 ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt tcaaatacca
    14641 tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg tatctttgat
    14701 gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat ttctgatttc
    14761 cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa tggaattgta
    14821 actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat atggacatct
    14881 attagtacta cggcttcaaa tatttggaac tcaattagtg caacagcttc tagtatttgg
    14941 aatagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt aattcaaaat
    15001 atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag ctctacagca
    15061 tcaaatatct ggagtagcgt gactagtagt ataagcaatg ctataaacgc agctaaaagt
    15121 gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg gaacggtatc
    15181 aaaaacactg ctttgaatct ttggaatgga attacaagca ctattagctc taaagtaaac
    15241 gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt ttccgacata
    15301 ttcaataatg ttaaaagcac gatagctaat atatgggaag gtatcaaaaa gactgttagc
    15361 gctccgattg attggattag agataaaatc agtagtgtct ttgataattt gaatatttct
    15421 ataccacata ttccgttacc acattttaaa ttgagcggag aattcaatcc attgaagggg
    15481 aaaatcccaa cattgggtgt tgattggtat gcgaaaggta gtgtgtttaa ttctccaaat
    15541 attatcggtg taggtgaatc aggacctgaa gcagttttac ctttgaaaag atctgtgctg
    15601 caagaaattg gtgatcgtat cttgagtagc acctcagttt catctaggac acaagcggtt
    15661 caacctgtga acaattacga attcaatttc acaattgatg gtaacgcaga tgaagtcact
    15721 atgaagcaaa caactcaaca aatcattgat agcattacaa aagttcaaaa tgataatgct
    15781 tcggcatggc gttaaacagg agagtatttc tcctgttttt ttagtattaa aaaggatgtg
    15841 aaaaaatgac tgattgtata cattctataa tcgatggatt tcctgattat ttgcataaat
    15901 tggctttagc tgaaagacca accatacctt ctccaaaaag acagagagtt gaaacttctg
    15961 ttttaggaag gttaggtggc ttagtgcaag attactcgtt tgaagacatg tcgtttacat
    16021 tgcactataa ctatttagag gatgtggaag accatcaagc gttcaagcaa tcgttttata
    16081 tcatgcgtca ttggttaaac tatgcaaaga aattagaatt ctctgacgat cccaacgtct
    16141 attacgttat ccagactatc gatattgggg atgcagaaaa cgatattgtt gaatggggag
    16201 agttcgatgt aaatattact gcgaaaccat tcgcaagagt tcaagaagat gtacctataa
    16261 ccgtagataa accacagtca tttaacttgc tgaataatag tttagaagaa agttttccaa
    16321 agattatcat cactccttca gctacttcat gccagttcat cttaaatgat tatgtgttta
    16381 gtttcgaagg cttagtagca ggaactgatg tagtcattga tagtgatttg atgctttgct
    16441 acgaagagca atcggacgga gatattttag atcggtccaa caaaatgaag accatgcaat
    16501 atccgacatt gcaagtggat atcaatcatt ttaattgcac tggtttgagc aaaatacaaa
    16561 tttatcgtaa cgggttaagg taggtgaaat agatgatcga taatttaata actatttacg
    16621 ataaaaatga cgcgaataat ttagctgaac atttatatga tacgcaaggt ttaggcgctt
    16681 tgtcagactg gttaacagct actgttagca ataaactaaa cggagccgag atatttcagg
    16741 gtacttatcc aataagcgga actaatgcag atttgattgt agaaggacgt attattcagt
    16801 gttatgtaga tgaaaatcga gcaaaacagc gtctacggat ttattatgca aaaacttctg
    16861 taataggcaa tacgatagaa gtaaaagctg aacctatttt caatgatata agaaaatcgg
    16921 tgttgaataa atatgacagc ggaacagaaa aaatcactgc tactcaggca tggcaaaacg
    16981 caaaagcttt agcgaaacca gctatccctt cgcagttttc tttctcgtca ttagtagata
    17041 cgcttgctaa tgtgaagata gaaaaagcga attttttaga attctttggt ggaaaagagg
    17101 gatctattct agatcgattt catggggaat ttcttaaaga taataacaca ttacatcatg
    17161 aaaaaaggct aggtacggat cataaaatca aagcgattta tactaaaaat ttaactggtc
    17221 ttgacttaga gatagatgct caaagcgttt tagttggagt ttatccattc attagcagtt
    17281 cttcagaagg agaagacgag atcactctac cagaagaagt cattttcacg gattacgtgg
    17341 atgattatcc tgctggatat gtttcttttg ttgattttaa agacaaagcg actgatgtag
    17401 ccacattaag ggaagctgct aaagactggt tgaaaacaaa catagataaa caaaaaccac
    17461 aagtgagtgg ttcgattgaa ttagtaccat tgaggcatca aagaggctat gaaaaatttg
    17521 ttgatctaga aaaagtttcg atgggtgacg gagtagatgt gtatcatcca cagttaaaag
    17581 tgaatatgtc agcgagaatt gtggaatata cgtttaatgt tctaaccaat tcatacgata
    17641 aattagttgt aggaaacgtc aaaacaaact tcttagaaaa cacagagaat aatgtaagca
    17701 atttgattaa tgatgccatt gatcaattga aaaatggtgg cgaaatcagt gatttaatca
    17761 atgatattgt agatcatcaa actgatatga ttactggtca aaatggtggt tatgttttat
    17821 tagatcctaa agaagcgcct agtcgtattt tgattatgga cacaccagat aagaataccg
    17881 caaggaatgt tttacaaatc aacaatgctg gtattggttt ttctaaaact ggcattaatg
    17941 gaacatatga aaccgcatgg acattagatg gcggattcaa tgcctcgttt attacggctg
    18001 gtgaaatagt aggaattact attagaggta ctacattaat tagtgatggc actgattata
    18061 gaacaagtat tgctaatggc aaaatgactt ggtactcaaa aaaagttaac aaagatatta
    18121 tggagctaga agcacgtgat tatgtaagtg ctgatgccgg tattgtatca tacaccatga
    18181 aaactggtgg tggtttcatg attagaaatc cacagggtaa cttggttttt agtacgtggg
    18241 ataatggtaa taacagaccg tttctatctt ttggtgcgcc caatttcagg tatagcaatg
    18301 ctagttatgt aacttctggc gacggtagtt ctttaagcat taatggtagt gcgggtaact
    18361 catgggaatt taaggtagct ggtaggacta tgaaatttac tagtgatggt atgctaacgt
    18421 tgccaggttg tttttttggt tcatgggaag atgggaaact tgctaggttt gaacaatcaa
    18481 cggtacaagt atataaagat tttactgtta gaggtactaa aaactcaacc gtaccaacag
    18541 aacattatgg acaacgacta ttaaacgctt atgaaactcc agaatattat ttcgctgatt
    18601 atggggaagc cgttacaggt gatgatggta aagttcgtgt tgatattgac cccatgtttg
    18661 ctgagacagt aaatctaagt cgatatatga cacatgtgac acctacagaa ctagttttgt
    18721 gtgctgttac tcatgaagat attgaccatt tcatcattga aactagtaag ccaaacgtat
    18781 tagttagatg gaatttagtg gcacaccgtc tagggtatga agatattaga ttaaaagagg
    18841 atacagcata tgatagcaca gtgcttgacc aaaaacgttt ttaaaacgaa gacaaggagg
    18901 tatataaatg gctagcagtt tatataattt ggctctagat ttcagcaaag aattaaacta
    18961 caccaaagct attatggctc gtcaaggtga taaagggatt acagtgactg ttaaaccatt
    19021 tttaaatggc ttgcagatgg atacgagtgg cggaacattt actttaaaag gaacaacgcc
    19081 atctaaccgt tacgtagata gtgtggcaac tagtgtaact agtgaagaag tcacgttttc
    19141 tcttgatggc acatttatga gtgaagcagg atattataaa cactgctacg tagaatatag
    19201 aaaagacaat caaattttaa caacgcaaga tatcattttt ttctcactag gagtgtctga
    19261 catttcgcaa ggccaagccg atgaatacgt ttcgcaattg gaagagttga ttcaaaagta
    19321 taacgaaact tttgatgctt ttatggctga aatcgaaggt agagtggaca gcttaaataa
    19381 acagattact gatttaactg gtcaagctaa aacactacaa gacaagttag acgctctgaa
    19441 agaagaaatt tctaaattag gtaacttgca ggtgatgtac agtaacagca tcga
//
LOCUS       DAZKAS010000039        19476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000039 DAZKAS010000000
VERSION     DAZKAS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.039"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8H7_001219"
     CDS             133..3660
                     /locus_tag="ACG8H7_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP8983327.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8H7_001220"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8H7_001220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFP8983328.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8H7_001221"
     CDS             7448..7789
                     /locus_tag="ACG8H7_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP8983329.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8H7_001222"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8H7_001222"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP8983330.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8H7_001223"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8H7_001223"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP8983331.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8H7_001224"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8H7_001224"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP8983332.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8H7_001225"
     CDS             13000..13737
                     /locus_tag="ACG8H7_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP8983333.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8H7_001226"
     CDS             13751..14566
                     /locus_tag="ACG8H7_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP8983334.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8H7_001227"
     CDS             14579..15226
                     /locus_tag="ACG8H7_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP8983335.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8H7_001228"
     CDS             15242..15898
                     /locus_tag="ACG8H7_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP8983336.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8H7_001229"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8H7_001229"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP8983337.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8H7_001230"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8H7_001230"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP8983338.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8H7_001231"
     CDS             18240..18758
                     /locus_tag="ACG8H7_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8983339.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8H7_001232"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8H7_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFP8983340.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6550 a         3344 c         4180 g         5402 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataa
//
LOCUS       DAZKAS010000040        19351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000040 DAZKAS010000000
VERSION     DAZKAS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.040"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACG8H7_001233"
     CDS             complement(407..766)
                     /locus_tag="ACG8H7_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP8983341.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACG8H7_001234"
     CDS             complement(796..1137)
                     /locus_tag="ACG8H7_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983342.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACG8H7_001235"
     CDS             complement(1134..1808)
                     /locus_tag="ACG8H7_001235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP8983343.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG8H7_001236"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG8H7_001236"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP8983344.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACG8H7_001237"
     CDS             complement(4322..5512)
                     /locus_tag="ACG8H7_001237"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP8983345.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACG8H7_001238"
     CDS             complement(5533..6060)
                     /locus_tag="ACG8H7_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFP8983346.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACG8H7_001239"
     CDS             complement(6107..8896)
                     /locus_tag="ACG8H7_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFP8983347.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACG8H7_001240"
     CDS             complement(9043..9243)
                     /locus_tag="ACG8H7_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8983348.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACG8H7_001241"
     CDS             complement(9696..10016)
                     /locus_tag="ACG8H7_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983349.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACG8H7_001242"
     CDS             complement(10519..11637)
                     /locus_tag="ACG8H7_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983350.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACG8H7_001243"
     CDS             11870..11992
                     /locus_tag="ACG8H7_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983351.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACG8H7_001244"
     CDS             12108..13415
                     /locus_tag="ACG8H7_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFP8983352.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACG8H7_001245"
     CDS             13536..14735
                     /locus_tag="ACG8H7_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFP8983353.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACG8H7_001246"
     CDS             complement(14762..16030)
                     /locus_tag="ACG8H7_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFP8983354.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACG8H7_001247"
     CDS             complement(16200..16841)
                     /locus_tag="ACG8H7_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFP8983355.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACG8H7_001248"
     CDS             complement(17250..17753)
                     /locus_tag="ACG8H7_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP8983356.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACG8H7_001249"
     CDS             complement(17876..18640)
                     /locus_tag="ACG8H7_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFP8983357.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACG8H7_001250"
     CDS             18748..19023
                     /locus_tag="ACG8H7_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP8983358.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5542 a         4126 c         3444 g         6239 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca t
//
LOCUS       DAZKAS010000041        19283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000041 DAZKAS010000000
VERSION     DAZKAS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.041"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG8H7_001251"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACG8H7_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP8983359.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG8H7_001252"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACG8H7_001252"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP8983360.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACG8H7_001253"
     CDS             complement(2303..2965)
                     /locus_tag="ACG8H7_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8983361.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACG8H7_001254"
     CDS             3149..4795
                     /locus_tag="ACG8H7_001254"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP8983362.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG8H7_001255"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACG8H7_001255"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP8983363.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="ACG8H7_001256"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="ACG8H7_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="ACG8H7_001257"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="ACG8H7_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="ACG8H7_001258"
     CDS             complement(8944..10023)
                     /locus_tag="ACG8H7_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8983364.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="ACG8H7_001259"
     CDS             complement(10126..10566)
                     /locus_tag="ACG8H7_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8983365.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="ACG8H7_001260"
     CDS             complement(10623..11033)
                     /locus_tag="ACG8H7_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP8983366.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="ACG8H7_001261"
     CDS             complement(11213..11647)
                     /locus_tag="ACG8H7_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8983367.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="ACG8H7_001262"
     CDS             complement(11747..12595)
                     /locus_tag="ACG8H7_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8983368.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="ACG8H7_001263"
     CDS             12984..14750
                     /locus_tag="ACG8H7_001263"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP8983369.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="ACG8H7_001264"
     CDS             14852..16111
                     /locus_tag="ACG8H7_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8983370.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="ACG8H7_001265"
     CDS             16258..17559
                     /locus_tag="ACG8H7_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8983371.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="ACG8H7_001266"
     CDS             17562..18413
                     /locus_tag="ACG8H7_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP8983372.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="ACG8H7_001267"
     CDS             18426..19241
                     /locus_tag="ACG8H7_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFP8983373.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         6355 a         3509 c         3577 g         5842 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atg
//
LOCUS       DAZKAS010000042        19167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000042 DAZKAS010000000
VERSION     DAZKAS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.042"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1509
                     /locus_tag="ACG8H7_001268"
     CDS             82..1509
                     /locus_tag="ACG8H7_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983374.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1601..1966)
                     /locus_tag="ACG8H7_001269"
     CDS             complement(1601..1966)
                     /locus_tag="ACG8H7_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP8983375.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1963..2328)
                     /locus_tag="ACG8H7_001270"
     CDS             complement(1963..2328)
                     /locus_tag="ACG8H7_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFP8983376.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2520..2768)
                     /locus_tag="ACG8H7_001271"
     CDS             complement(2520..2768)
                     /locus_tag="ACG8H7_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983377.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2767..2895
                     /locus_tag="ACG8H7_001272"
     CDS             2767..2895
                     /locus_tag="ACG8H7_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFP8983378.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2952..4646
                     /locus_tag="ACG8H7_001273"
     CDS             2952..4646
                     /locus_tag="ACG8H7_001273"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFP8983379.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4746..4919
                     /locus_tag="ACG8H7_001274"
     CDS             4746..4919
                     /locus_tag="ACG8H7_001274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983380.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5297..6430
                     /locus_tag="ACG8H7_001275"
     CDS             5297..6430
                     /locus_tag="ACG8H7_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP8983381.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6461..6781
                     /locus_tag="ACG8H7_001276"
     CDS             6461..6781
                     /locus_tag="ACG8H7_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFP8983382.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6810..7274)
                     /locus_tag="ACG8H7_001277"
     CDS             complement(6810..7274)
                     /locus_tag="ACG8H7_001277"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8983383.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7406..8659)
                     /locus_tag="ACG8H7_001278"
     CDS             complement(7406..8659)
                     /locus_tag="ACG8H7_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8983384.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8688..9005)
                     /locus_tag="ACG8H7_001279"
     CDS             complement(8688..9005)
                     /locus_tag="ACG8H7_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP8983385.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9031..9351)
                     /locus_tag="ACG8H7_001280"
     CDS             complement(9031..9351)
                     /locus_tag="ACG8H7_001280"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8983386.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9815..11230)
                     /locus_tag="ACG8H7_001281"
     CDS             complement(9815..11230)
                     /locus_tag="ACG8H7_001281"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8983387.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11247..12563)
                     /locus_tag="ACG8H7_001282"
     CDS             complement(11247..12563)
                     /locus_tag="ACG8H7_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8983388.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12713..13411)
                     /locus_tag="ACG8H7_001283"
     CDS             complement(12713..13411)
                     /locus_tag="ACG8H7_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP8983389.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13700..14689)
                     /locus_tag="ACG8H7_001284"
     CDS             complement(13700..14689)
                     /locus_tag="ACG8H7_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP8983390.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14769..15602)
                     /gene="msrA"
                     /locus_tag="ACG8H7_001285"
     CDS             complement(14769..15602)
                     /gene="msrA"
                     /locus_tag="ACG8H7_001285"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP8983391.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15777..17411)
                     /locus_tag="ACG8H7_001286"
     CDS             complement(15777..17411)
                     /locus_tag="ACG8H7_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFP8983392.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17398..>19167)
                     /locus_tag="ACG8H7_001287"
     CDS             complement(17398..>19167)
                     /locus_tag="ACG8H7_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983393.1"
                     /translation="KNLTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLS
                     TLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLT
                     IIPAVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFAT
                     YRTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTL
                     LCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEI
                     HSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIY
                     VGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKY
                     SWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPT
                     EAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKV
                     NGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRF
                     APNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6085 a         3748 c         3188 g         6146 t
ORIGIN      
        1 aattaactga tgatttcaaa aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa
       61 atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag
      121 ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat
      181 ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt
      241 gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa
      301 catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact
      361 tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc
      421 atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttactt taatgactgc
      481 gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt
      541 cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt
      601 gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa
      661 gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt
      721 gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat
      781 gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc
      841 tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg
      901 gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta
      961 gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt
     1021 gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa
     1081 ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc
     1141 agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt
     1201 gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc
     1261 acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc
     1321 gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag
     1381 cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat
     1441 gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa
     1501 aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca
     1561 tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta
     1621 ggggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg
     1681 ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa
     1741 caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct
     1801 cgcttactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga
     1861 ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa
     1921 gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct
     1981 tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat
     2041 gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat
     2101 gatatcagca acgactagta cttccaagct taacaaaata taacttgcta gcagttttct
     2161 tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa
     2221 aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa
     2281 aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta
     2341 ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt
     2401 taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt
     2461 ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct
     2521 tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt
     2581 ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt
     2641 acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc
     2701 atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg
     2761 tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc
     2821 gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa
     2881 ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg
     2941 gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg
     3001 cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt
     3061 cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt
     3121 ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc
     3181 ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg
     3241 agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg
     3301 acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca
     3361 ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa
     3421 aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa
     3481 acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat
     3541 tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg
     3601 tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga
     3661 aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa
     3721 caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag
     3781 aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta
     3841 cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg
     3901 cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca
     3961 aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc
     4021 atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt
     4081 catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg
     4141 tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt
     4201 atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg
     4261 gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga
     4321 atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata
     4381 tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc
     4441 ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga
     4501 tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta
     4561 ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc
     4621 gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag
     4681 taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac
     4741 cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc
     4801 aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat
     4861 taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt
     4921 ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact
     4981 taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt
     5041 tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata
     5101 caactatttc aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa
     5161 atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta
     5221 agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct
     5281 gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag
     5341 aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac
     5401 agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg
     5461 gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac
     5521 attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt
     5581 ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg
     5641 gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag
     5701 agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa
     5761 agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt
     5821 aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa
     5881 attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct
     5941 agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc
     6001 tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg
     6061 aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg
     6121 cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt
     6181 aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga
     6241 tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat
     6301 gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga
     6361 agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt
     6421 gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa
     6481 tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg
     6541 cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat
     6601 tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta
     6661 tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa
     6721 catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg
     6781 agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga
     6841 gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa
     6901 aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt
     6961 ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac
     7021 agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc
     7081 gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc
     7141 ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt
     7201 gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag
     7261 ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa
     7321 gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat
     7381 cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga
     7441 tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg
     7501 ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta
     7561 cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa
     7621 cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga
     7681 taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt
     7741 ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac
     7801 ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga
     7861 taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat
     7921 tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga
     7981 atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg
     8041 gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa
     8101 ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg
     8161 cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc
     8221 ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt
     8281 ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg
     8341 cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca
     8401 agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt
     8461 caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca
     8521 ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg
     8581 caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa
     8641 atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat
     8701 cgacgataca gattaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga
     8761 tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt
     8821 gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt
     8881 ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg
     8941 ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct
     9001 tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc
     9061 gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat
     9121 gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa
     9181 aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc
     9241 aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag
     9301 acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac
     9361 gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt
     9421 taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga
     9481 aagcgcacac taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa
     9541 aaaagttgtg acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg
     9601 catctgcggt aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat
     9661 tcttattact tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag
     9721 ctgaccctgg gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca
     9781 gtaaatacaa tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat
     9841 ccaattctct cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc
     9901 ttcgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg
     9961 gtgacacaca gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg
    10021 ttgctttgat caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca
    10081 cgattccttt ctcatccata tactgttctt catattctct tcccataccg ttttctgtta
    10141 tcagccaagg gatattatga taattttctt ttagatacat ggctatatca tacatgattt
    10201 tcggatagat ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt
    10261 cataatatcg ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa
    10321 cgcgtttagg ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt
    10381 ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact
    10441 cttctgtgta gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat
    10501 acatataagc agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat
    10561 ttaaaataca gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag
    10621 ccgttgctaa aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg
    10681 gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa
    10741 accagttttt taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa
    10801 aatcaactac ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt
    10861 cataatgttc caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt
    10921 cacttatatg ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac
    10981 gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat
    11041 cctgctggta ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa
    11101 caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt
    11161 tccaaccttc cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag
    11221 taattttcat gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact
    11281 tttacaaatg gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact
    11341 gcaccgatat ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg
    11401 accattacaa ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca
    11461 gtgataagag gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata
    11521 ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt
    11581 tgtttcatat cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact
    11641 ccagacccgc ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact
    11701 tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc
    11761 cgtatcggct tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata
    11821 aacatcaata gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg
    11881 acattgtaaa tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca
    11941 gccgcgatac ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca
    12001 ttcgttggca cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa
    12061 taaagttcaa tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta
    12121 tattgtttag ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa
    12181 acagttggat taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc
    12241 actttattca gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa
    12301 cttagtgtga catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag
    12361 aaacttatct gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca
    12421 aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata
    12481 taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata
    12541 aaactcattc cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct
    12601 attagtcagc gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata
    12661 taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag
    12721 tttctttttc tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca
    12781 ttgcatcaaa cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa
    12841 tatcgatcgt ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg
    12901 attcattaat gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca
    12961 tcgtatgaaa atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt
    13021 caatcttttg cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg
    13081 ccaaacctgc catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc
    13141 gatcctgatc ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac
    13201 tgacaaatgt ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca
    13261 cagcttctcg caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg
    13321 gcagtttatc tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag
    13381 ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat
    13441 tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga
    13501 ttaatccgtt catagacata caaacgaaga agatatttac cataaaaaag actaggataa
    13561 aattcactcc tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata
    13621 acagacgatc ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa
    13681 aagtttgatt acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg
    13741 agaaatctaa attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc
    13801 cgaccagtac acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta
    13861 atgattgtcc tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc
    13921 caacttgttc ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa
    13981 taccatgtag ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc
    14041 ctagcccttc ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca
    14101 tattgtagcg catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca
    14161 ttttttttgt atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc
    14221 ttacaagttg catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat
    14281 gatgatagaa gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg
    14341 aaatgatgga tttacgagag ccccattccc catatggccc ttcccacata tgataacctg
    14401 ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc
    14461 caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg
    14521 taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat
    14581 caccaaaatt ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg
    14641 atttacgata ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc
    14701 ctcatcaaat ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag
    14761 aaaaggagtt aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt
    14821 tgatagatca gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt
    14881 ttgtaaaatt gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt
    14941 gtgacgattg gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct
    15001 aattcttttt gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct
    15061 tgaaattgtc caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg
    15121 ctataactta tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa
    15181 ccaccactta cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa
    15241 acagatagga tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct
    15301 gcaaatactg cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat
    15361 ttttgctcct tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc
    15421 agattcattc ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta
    15481 acggatatat tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct
    15541 cgtgtattcg gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc
    15601 atagattctc ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa
    15661 agtgaggaaa taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa
    15721 tccttatcga cagattctcc tttttaatca atttatttta taaatgaaaa gttaatttac
    15781 cttttatgga aaactatttt aaaaaataaa cataaatatg cagttaatgt aacaagataa
    15841 gaaataatag tcgttactgt ataacttgtg cttactttaa tatcagcaac tcctttgact
    15901 ccgcttattt gtaccaatcc attagcattc acctgctcct ttacaggttg atgatttatt
    15961 tctacattcg ttcctaaata agcatataca ggaagtgtga ttacttggct cttctcatta
    16021 gttactctat atatgataga actaccagtt gttttataag gaaggtttac ccattgactg
    16081 ccaatctttg cttcgtgttt tattatatgc tccttattct ccaatgaagc gataggagaa
    16141 taatcatata aaaaataatc cattccttta gtggtatacg cctctactgt ttctttgttt
    16201 acaacaatat gtcccggctg agaaattatt ttttgattga aatttttttt actagaaaca
    16261 gttaaagtga ttaataacaa aacagtcata aataaataaa atagttctct tgtcttattg
    16321 tatcttctat ttttgaaaaa tatcatcgtt aatatcagag aagagaatat tatttgcaag
    16381 cttaagatcc gccaaggaaa ttgtaacaat ttggctggcg tattttgcag aagataccat
    16441 ggcaccaaac tagttgtagc tagccaagtc aaacacgtac cataataaat ctttttttct
    16501 atgtttgtta attttttaaa taaaatcagg ggaatacata aagataacag accaactagt
    16561 cccattgagt aagtagttaa gtcgttgttc atagcttcag tcagactatc gaacacatta
    16621 gatgcatgag catataaatt tgtcacgcta ggtttgttga ttttttggta gagcatttgt
    16681 tcaaacatcg gataccaaaa agcactcgtc agaaccaatg tcattcctgc cgcactaaat
    16741 aattttttaa atatagcaat aaaatctgca ttcttgaaac ctttagaaac caaacgaata
    16801 aaaataaaaa tgataattaa tgctgtactc attaatgttg atagtacatg gctgtacact
    16861 agcaaactca tactcaatgc tagctttaca gacggatact tttcttcttt tccagagatg
    16921 atttgataag cataaaataa tacagggata acaagtgtta ttgcgatagc ttcggcaatt
    16981 gcggaacgat agtagatgtc tactgtccga taagcagaga aaatatagag acaactaaat
    17041 aaaaatgccg cttttttttg ttttaaaaac cgctcgccat aatgataagc tacgagtgtt
    17101 gtacacaagt tcaataacca aacgtaaata atatagctta caatcaaatt ttttgtcatc
    17161 caatataaca ttacagctgg aaaaaaagtc agaaagggat aaaaataatt tacaccatat
    17221 ccagtataat tgaatgtctc ataattaatc ggactggtaa aaatagtcga taaccccttg
    17281 attcgagata agtgaaaaga aagatctcca gtggtataaa tactctgtgt atttttgaaa
    17341 aagatgaaag aatatatact actaactata attaatccac tcaatattac tactttttta
    17401 tttattttca ttcttcttgt ttatctcctt tttagtcaca tatcgagaat ctttcaactt
    17461 attaggtgca aatcgtttaa aacttgaata caccactaaa caataagtca gaatactgat
    17521 ccataaagcc atgagagcag ttggagtata ttttgttgta atttctatct tattttctcc
    17581 agcatttaca tccacattca ctaagccatg ttttttataa gagtcattta cgactttacc
    17641 gtttactttt acctgtgtac ctaaataagc atatactggt actgtcactt tttgcttttt
    17701 gttgcttgcc actgtgtaag taatgttatt atctgcagct ttatacttcc ctttttccca
    17761 cgattctcct acttttactt catgtttttc taagtgaggc ctttctttta tcgcttctgt
    17821 tggggcgtaa tcgtataatc ctcccatatc tgatgttgta taacgtggga gtgtttcttc
    17881 attgataaca atatgaccgt gttttgattg gataactttt gcgtaatttt cttttgctgc
    17941 tatagacgtg acgagtagaa acaccacagt agcgccaatg attaaccatg aatatttttt
    18001 attcatagtc tttttcataa aaacaagcgt tatgatcatt gaagaaaaaa agatttggat
    18061 acctaagata cgccaaggaa actggagtaa gcttatcggt gttttttgaa acaaaaacca
    18121 tggaaataaa ttagttacgc atacccatgt agaaactacg ccaacataga tgattttttc
    18181 ttttttctcc atttctttca gtatgaataa aggaaaaatc aacgcaacca atcctaataa
    18241 ccccatagtg tatgttgtaa tatcgttatt taacgcttga atgatactat ctgaaataga
    18301 aagtgcttct agctgcaaat ctgttttgaa gggactgtgt atttcttgat aattcatctg
    18361 ctgcaacata ggataccaaa aatagctagt taatgcacaa gtcaaaaaaa cagcctttac
    18421 taaattcacg accacaaatt ttatatcgtt ttttatttgc ttgcttaaaa acaaacgaac
    18481 tataatcata caagcaatca atgcactaca caatagtgta gttaagacat gagtatacac
    18541 aagtagactt atactgacag ataaagcgat gacttttttt tctctattga atatcaatcc
    18601 ataagcataa tataaaacag ctgggataat cgtaagagcg attgcttctg caattgcgga
    18661 tcgataatag atatcaactg ttcgataagt tgcaaaagta tataaacaac taaataaaaa
    18721 agcagcatct atctttttaa aaaacttttc tccataataa aaactaatca gaatcgtaca
    18781 aatatttaat aaccaaatat atagaacata acttaatatt aagttatttg aaattccata
    18841 aaaaacgact gcaggaatta ttgtaagaaa aggataaaaa tagttcagtc catctccata
    18901 attgttaaat gttgtatagt tgattggccc ttcaaaaata gaagataacc ctttgattcg
    18961 tgaaagatga aaagataaat cacctgttgt ataaatttct tgagcatttc tcataaaatt
    19021 tactgaatat aaagtactaa gtaagataag aactactaaa agaaatgctt ttttacttat
    19081 tttcatagat ttgttgttct ccttttttaa ttctattttc agtatactag aattgtcttt
    19141 ttttaataag ttttgcgtta aattttt
//
LOCUS       DAZKAS010000043        19126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000043 DAZKAS010000000
VERSION     DAZKAS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.043"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACG8H7_001288"
     CDS             complement(<1..703)
                     /locus_tag="ACG8H7_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983394.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACG8H7_001289"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACG8H7_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACG8H7_001290"
     CDS             1758..2837
                     /locus_tag="ACG8H7_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP8983395.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACG8H7_001291"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACG8H7_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACG8H7_001292"
     CDS             complement(3572..3799)
                     /locus_tag="ACG8H7_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8983396.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACG8H7_001293"
     CDS             complement(3902..6490)
                     /locus_tag="ACG8H7_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFP8983397.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACG8H7_001294"
     CDS             complement(6510..7169)
                     /locus_tag="ACG8H7_001294"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP8983398.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG8H7_001295"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACG8H7_001295"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP8983399.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACG8H7_001296"
     CDS             complement(7942..8808)
                     /locus_tag="ACG8H7_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP8983400.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACG8H7_001297"
     CDS             8917..9558
                     /locus_tag="ACG8H7_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP8983401.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG8H7_001298"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACG8H7_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFP8983402.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACG8H7_001299"
     CDS             complement(10999..11898)
                     /locus_tag="ACG8H7_001299"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP8983403.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACG8H7_001300"
     CDS             complement(11900..12697)
                     /locus_tag="ACG8H7_001300"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFP8983404.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACG8H7_001301"
     CDS             complement(12675..13379)
                     /locus_tag="ACG8H7_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFP8983405.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACG8H7_001302"
     CDS             13510..14085
                     /locus_tag="ACG8H7_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFP8983406.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACG8H7_001303"
     CDS             14203..14880
                     /locus_tag="ACG8H7_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFP8983407.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG8H7_001304"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACG8H7_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFP8983408.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACG8H7_001305"
     CDS             complement(16943..17941)
                     /locus_tag="ACG8H7_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP8983409.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACG8H7_001306"
     CDS             complement(18167..18820)
                     /locus_tag="ACG8H7_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFP8983410.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DAZKAS010000044        18740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000044 DAZKAS010000000
VERSION     DAZKAS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.044"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..531
                     /locus_tag="ACG8H7_001307"
     CDS             256..531
                     /locus_tag="ACG8H7_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983411.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ACG8H7_001308"
     CDS             544..2616
                     /locus_tag="ACG8H7_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP8983412.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2784..4667
                     /locus_tag="ACG8H7_001309"
     CDS             2784..4667
                     /locus_tag="ACG8H7_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP8983413.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4679..5626
                     /locus_tag="ACG8H7_001310"
     CDS             4679..5626
                     /locus_tag="ACG8H7_001310"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP8983414.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ACG8H7_001311"
     CDS             5646..6173
                     /locus_tag="ACG8H7_001311"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP8983415.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ACG8H7_001312"
     CDS             complement(6236..6889)
                     /locus_tag="ACG8H7_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP8983416.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ACG8H7_001313"
     CDS             7021..7863
                     /locus_tag="ACG8H7_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP8983417.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ACG8H7_001314"
     CDS             8021..8917
                     /locus_tag="ACG8H7_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP8983418.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ACG8H7_001315"
     CDS             8920..9633
                     /locus_tag="ACG8H7_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFP8983419.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ACG8H7_001316"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ACG8H7_001316"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP8983420.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ACG8H7_001317"
     CDS             10168..10395
                     /locus_tag="ACG8H7_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFP8983421.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ACG8H7_001318"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ACG8H7_001318"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP8983422.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="ACG8H7_001319"
     CDS             complement(11181..11723)
                     /locus_tag="ACG8H7_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFP8983423.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ACG8H7_001320"
     CDS             complement(11960..12358)
                     /locus_tag="ACG8H7_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8983424.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ACG8H7_001321"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ACG8H7_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFP8983425.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ACG8H7_001322"
     CDS             13471..14238
                     /locus_tag="ACG8H7_001322"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP8983426.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ACG8H7_001323"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ACG8H7_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP8983427.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ACG8H7_001324"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ACG8H7_001324"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP8983428.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ACG8H7_001325"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ACG8H7_001325"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFP8983429.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAZKAS010000045        18164 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000045 DAZKAS010000000
VERSION     DAZKAS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.045"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACG8H7_001326"
     CDS             <1..152
                     /locus_tag="ACG8H7_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8983430.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACG8H7_001327"
     CDS             complement(230..763)
                     /locus_tag="ACG8H7_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFP8983431.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG8H7_001328"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG8H7_001328"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP8983432.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG8H7_001329"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG8H7_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP8983433.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG8H7_001330"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG8H7_001330"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP8983434.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACG8H7_001331"
     CDS             complement(4488..5066)
                     /locus_tag="ACG8H7_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFP8983435.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ACG8H7_001332"
     CDS             5388..5858
                     /locus_tag="ACG8H7_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFP8983436.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ACG8H7_001333"
     CDS             complement(5966..7192)
                     /locus_tag="ACG8H7_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP8983437.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ACG8H7_001334"
     CDS             complement(7946..8146)
                     /locus_tag="ACG8H7_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8983438.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ACG8H7_001335"
     CDS             complement(8280..8888)
                     /locus_tag="ACG8H7_001335"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFP8983439.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ACG8H7_001336"
     CDS             complement(8973..9443)
                     /locus_tag="ACG8H7_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFP8983440.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ACG8H7_001337"
     CDS             complement(9491..10405)
                     /locus_tag="ACG8H7_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFP8983441.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ACG8H7_001338"
     CDS             10551..11354
                     /locus_tag="ACG8H7_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFP8983442.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ACG8H7_001339"
     CDS             11532..12479
                     /locus_tag="ACG8H7_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP8983443.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ACG8H7_001340"
     CDS             complement(12547..12840)
                     /locus_tag="ACG8H7_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFP8983444.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ACG8H7_001341"
     CDS             complement(12868..13533)
                     /locus_tag="ACG8H7_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFP8983445.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ACG8H7_001342"
     CDS             complement(13673..14305)
                     /locus_tag="ACG8H7_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8983446.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACG8H7_001343"
     CDS             complement(14312..15379)
                     /gene="liaS"
                     /locus_tag="ACG8H7_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFP8983447.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACG8H7_001344"
     CDS             complement(15376..16107)
                     /gene="liaF"
                     /locus_tag="ACG8H7_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFP8983448.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACG8H7_001345"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ACG8H7_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP8983449.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACG8H7_001346"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ACG8H7_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFP8983450.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5389 a         3684 c         2905 g         6186 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgt
//
LOCUS       DAZKAS010000046        17980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000046 DAZKAS010000000
VERSION     DAZKAS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.046"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACG8H7_001347"
     CDS             complement(29..727)
                     /locus_tag="ACG8H7_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFP8983451.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACG8H7_001348"
     CDS             complement(720..2072)
                     /locus_tag="ACG8H7_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFP8983452.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACG8H7_001349"
     CDS             complement(2295..3695)
                     /locus_tag="ACG8H7_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFP8983453.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACG8H7_001350"
     CDS             3856..4593
                     /locus_tag="ACG8H7_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP8983454.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACG8H7_001351"
     CDS             4718..5197
                     /locus_tag="ACG8H7_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFP8983455.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACG8H7_001352"
     CDS             5194..5961
                     /locus_tag="ACG8H7_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFP8983456.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACG8H7_001353"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACG8H7_001353"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP8983457.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACG8H7_001354"
     CDS             complement(6864..7466)
                     /locus_tag="ACG8H7_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFP8983458.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACG8H7_001355"
     CDS             7572..7802
                     /locus_tag="ACG8H7_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983459.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACG8H7_001356"
     CDS             7793..8041
                     /locus_tag="ACG8H7_001356"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8983460.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACG8H7_001357"
     CDS             8147..8731
                     /locus_tag="ACG8H7_001357"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP8983461.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACG8H7_001358"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACG8H7_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP8983462.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACG8H7_001359"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACG8H7_001359"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP8983463.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACG8H7_001360"
     CDS             10632..10811
                     /locus_tag="ACG8H7_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983464.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACG8H7_001361"
     CDS             10833..11858
                     /locus_tag="ACG8H7_001361"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP8983465.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACG8H7_001362"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACG8H7_001362"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP8983466.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACG8H7_001363"
     CDS             13430..14377
                     /locus_tag="ACG8H7_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFP8983467.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACG8H7_001364"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACG8H7_001364"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP8983468.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACG8H7_001365"
     CDS             complement(15279..15521)
                     /locus_tag="ACG8H7_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983469.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACG8H7_001366"
     tRNA            15642..15714
                     /locus_tag="ACG8H7_001366"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACG8H7_001367"
     CDS             complement(15833..16471)
                     /locus_tag="ACG8H7_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP8983470.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16594..17226)
                     /locus_tag="ACG8H7_001368"
     CDS             complement(16594..17226)
                     /locus_tag="ACG8H7_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP8983471.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17577..17876
                     /locus_tag="ACG8H7_001369"
     CDS             17577..17876
                     /locus_tag="ACG8H7_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP8983472.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6157 a         3150 c         3554 g         5119 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    16681 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    16741 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    16801 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    16861 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    16921 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    16981 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    17041 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    17101 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    17161 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    17221 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    17281 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    17341 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    17401 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    17461 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    17521 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    17581 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    17641 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    17701 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    17761 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    17821 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    17881 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    17941 tttatttgaa cagagtctgt atttttttat tctaaataaa
//
LOCUS       DAZKAS010000047        17205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000047 DAZKAS010000000
VERSION     DAZKAS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.047"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..324
                     /locus_tag="ACG8H7_001370"
     CDS             64..324
                     /locus_tag="ACG8H7_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983473.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(686..925)
                     /locus_tag="ACG8H7_001371"
     CDS             complement(686..925)
                     /locus_tag="ACG8H7_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983474.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1211..1525
                     /locus_tag="ACG8H7_001372"
     CDS             1211..1525
                     /locus_tag="ACG8H7_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983475.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1959..2325)
                     /gene="ssrA"
                     /locus_tag="ACG8H7_001373"
     tmRNA           complement(1959..2325)
                     /gene="ssrA"
                     /locus_tag="ACG8H7_001373"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2496..3269)
                     /locus_tag="ACG8H7_001374"
     CDS             complement(2496..3269)
                     /locus_tag="ACG8H7_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8983476.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3413..3763
                     /locus_tag="ACG8H7_001375"
     CDS             3413..3763
                     /locus_tag="ACG8H7_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFP8983477.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3744..4460
                     /locus_tag="ACG8H7_001376"
     CDS             3744..4460
                     /locus_tag="ACG8H7_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP8983478.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4460..5908
                     /locus_tag="ACG8H7_001377"
     CDS             4460..5908
                     /locus_tag="ACG8H7_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8983479.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5978..6487)
                     /locus_tag="ACG8H7_001378"
     CDS             complement(5978..6487)
                     /locus_tag="ACG8H7_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983480.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6477..7202)
                     /locus_tag="ACG8H7_001379"
     CDS             complement(6477..7202)
                     /locus_tag="ACG8H7_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983481.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7425..9545)
                     /locus_tag="ACG8H7_001380"
     CDS             complement(7425..9545)
                     /locus_tag="ACG8H7_001380"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP8983482.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9775..10953
                     /locus_tag="ACG8H7_001381"
     CDS             9775..10953
                     /locus_tag="ACG8H7_001381"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFP8983483.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10969..11727
                     /gene="aroD"
                     /locus_tag="ACG8H7_001382"
     CDS             10969..11727
                     /gene="aroD"
                     /locus_tag="ACG8H7_001382"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP8983484.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11750..12235
                     /gene="ybaK"
                     /locus_tag="ACG8H7_001383"
     CDS             11750..12235
                     /gene="ybaK"
                     /locus_tag="ACG8H7_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFP8983485.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12306..13559)
                     /locus_tag="ACG8H7_001384"
     CDS             complement(12306..13559)
                     /locus_tag="ACG8H7_001384"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP8983486.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13677..15026
                     /gene="gdhA"
                     /locus_tag="ACG8H7_001385"
     CDS             13677..15026
                     /gene="gdhA"
                     /locus_tag="ACG8H7_001385"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP8983487.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            15138..16484
                     /locus_tag="ACG8H7_001386"
     CDS             15138..16484
                     /locus_tag="ACG8H7_001386"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP8983488.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16792..17178)
                     /locus_tag="ACG8H7_001387"
     CDS             complement(16792..17178)
                     /locus_tag="ACG8H7_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP8983489.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5653 a         2998 c         3425 g         5129 t
ORIGIN      
        1 acaatcatat tttaatggta ttatcatttt gaataatgaa aaaacattaa ggatggatga
       61 aatatgaata acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg
      121 aaacaaaaag tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta
      181 gacgaatatc atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca
      241 aaatatactc acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa
      301 tatgaacata ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc
      361 aagcttttga aggcacttta tttagttggc agtgattgtt catgattttt gatttattca
      421 aaaggttcgc ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa
      481 cctatactgc cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca
      541 tccgatccaa tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg
      601 ttatcgtaaa gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca
      661 ttatttgctg aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg
      721 ctgacagttt ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta
      781 ccggagaatt tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat
      841 ctgaaggtat gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata
      901 aactgataaa cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac
      961 acctatgctc agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat
     1021 tagtcttagt aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt
     1081 taccatctgt attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg
     1141 aaaacgtagg tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg
     1201 aggatagctt ttggattttt acagtaattt tatcttaata atagcaatat tattacttct
     1261 aaatatttgg ttttttgata agagtagaaa tgcagggata ggttttagaa ccaaacgaag
     1321 tacgtcttct gaaaaaaagt gggtatattc acagactatt ttttacggag gggttatttc
     1381 aatcagttta ctttcttcaa cactttattc ttttaatgtt atagacgttt ccatgtcaaa
     1441 ttttatatct attattggaa tccttatttc tgcaattatt acacaactac ttcttgtgtt
     1501 tgaagagaag tcaaaaaata attaaagcaa ttatgccttt tagtacagga attattactt
     1561 cttacaaatg tatagcgaat gtgaagcctg ctgttttaat tgttctagaa aaacttttga
     1621 gtgaacaatt attttatgtt aggatgaatt aatttctcta gagtttataa ttaaaaaact
     1681 tatttgaagg aacatgctta aaccataatt gtgtttttcc ttccatcagc ataaaagttc
     1741 acctcaaaaa gagtgtacga acaaaagttc tttttgtaaa ccttaaaata gattttcctg
     1801 aatacgtatt ggggatttct aaatgtttga atgttttttt gaatgcaaaa tcaatgcaaa
     1861 aaattttgta tttctctact tttctctcct tgaccaaaat aaaaaaacgt tgatttaaca
     1921 acgtttttca tacatgttta tctgtctcaa ccatacatat atggagacgg cgggagtcga
     1981 acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga
     2041 ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt
     2101 tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca
     2161 catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt
     2221 gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa
     2281 acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg
     2341 ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa
     2401 gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat
     2461 tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat
     2521 gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat
     2581 ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca
     2641 ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga
     2701 cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca
     2761 ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat
     2821 aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca
     2881 tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt
     2941 ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt
     3001 tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa
     3061 gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct
     3121 gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata
     3181 gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca
     3241 aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt
     3301 tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg
     3361 ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt
     3421 tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa
     3481 aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg
     3541 gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt
     3601 gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct
     3661 gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga
     3721 cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt
     3781 ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg
     3841 gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag
     3901 acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa
     3961 gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct
     4021 tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc
     4081 gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagcagttt
     4141 cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag
     4201 cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta
     4261 ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg
     4321 attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa
     4381 aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg
     4441 atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tttgcggaat
     4501 ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat
     4561 tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca
     4621 aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct
     4681 gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga
     4741 acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg
     4801 gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca
     4861 agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc
     4921 acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa
     4981 cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc
     5041 agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg
     5101 taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga
     5161 tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa
     5221 tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat
     5281 caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc
     5341 gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga
     5401 ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga
     5461 aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt
     5521 gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat
     5581 catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac
     5641 tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc
     5701 tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa
     5761 tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca
     5821 taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt
     5881 gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat
     5941 cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat
     6001 caatataagg actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt
     6061 cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt
     6121 catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt
     6181 gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag
     6241 agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat
     6301 ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga
     6361 tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat
     6421 gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag
     6481 gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat
     6541 agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt
     6601 atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc
     6661 ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc
     6721 tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg
     6781 atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata
     6841 aaaaggaata atttggtttt agaaagacca gtcaggcgcc aattgatcaa ttcctgcttg
     6901 ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa
     6961 ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta
     7021 gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc
     7081 aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa
     7141 tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc
     7201 atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt
     7261 tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa
     7321 gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga
     7381 gcagcgacaa taattttgtc gctgctcctt ttaaatttat tttgtcattc ggaagaactt
     7441 gtggtcgttc cgtgaagttc ttggtacgtt tgcgttttat aaagatcaac agttgattgt
     7501 ccgttgtgtt cactaaagac actagttgac ttgtcaccaa gagatttttc agcttctttt
     7561 aatttctcta agccattctt gtagtcataa tcactggtat caattgggtc taatccacta
     7621 ttcgtataga accgaagaag atcaccatta ttgatttggt cagaaacagc taactgttta
     7681 tcaacttttt ctttcagtga atcaatttca ttctgtactt cctcagtagg ttcagtaata
     7741 gcttcaccag tttcgttact atagatcgta ccaccgtagt aagtatattt tggtgtaaca
     7801 aaatcaccat cacggaaaga aacaagctgt tcatgatctt tcgagaatag atcttgtcct
     7861 aactgaatat aattttttgt atctacacct agtaagtgaa gtaatgtagg caaggcatca
     7921 acttgtccgc cgtaagtgtg attgattccg ccctgtgttt gtccagggat atggatcata
     7981 taagggacac gctgcatttg tgcattgtcg tagtttgtcc aagtagaact tgtttttcca
     8041 actaattcag ctagattttt atttcttgaa ttcgagacac cgtaatggtc accgtaaagg
     8101 acgatgactg aattatcata caagccgctt tctttcaaat aattgaagaa ttcttgtacg
     8161 gcggtatcta aataattggc tgtcgcaaag taaccgttga tcgtttcatc atttgtattg
     8221 gctaacggga atcctgcttc gtcattggtg aattttgaat aaggataatg gttagataca
     8281 gcaatgaatt ttgaataaaa tggttgttgc agacgttcca agtattgtac ggattgttgg
     8341 aaaaatggtt tatcatgcaa cccgtattgg aacgaattgt catcattaac atcataataa
     8401 gaagaatcaa agaaataatc atatcctaaa ttcttgtatg tctcgtttcg attccagaat
     8461 gtaccagcgt tcccatggaa taccgcacta gtgtaacctt gtgtttgact taaaatatca
     8521 ggtgcagctt ggaaagtatt ttttccgcct aattgtgtga acaaggagcc ttgattcaag
     8581 ccaaacaatg aattttccat cagcgtttcc gcatcgcttg tttttcctgc ttttacttga
     8641 tggaaaaagt tgtcaaaact gaacgtgctg ttgctgtggt aaagactatt gatgaaaggt
     8701 gtaacttcat gctggagacc attctcatct gtcaatttat aatcgatcag gaattgctgg
     8761 aagctttcta aatggatata aatcacattt tttcctttag cgagcccgaa caaatcgtca
     8821 tttggtgctg cataatgttc ttttacataa ttttctactt ctttcatatc attgggactt
     8881 gcttctgccc gcacttggtt tgtctggtaa gtttgaacac cgtcataaac catgaaagca
     8941 ttcaagccaa gatatttgac caaataatca cgagagaatg ttcgagtcag caattctgga
     9001 cgatcagctt ccgccataaa aagatttcct gagaaaatca taactgctaa tgtagagatc
     9061 gcaaaagcca ttcttgctcg gatcgggcga gggtccaact tgattttttt tgcaatcagc
     9121 agaacagtca agataataaa atcgatccaa tagatcacat catatgggcg aaacagtcga
     9181 atggcacttt ctcccagacc actcgctact ttcccagcac ctaaaatcgt gttgatcgta
     9241 atgaagtccg tgaattctcg ataataagtg acattcgaaa aaacaagcaa tgtcaaaaga
     9301 aaataaatga ccatcatcgt gatataagca actctagggc gtctgatata aagagcaatt
     9361 ccaagtaata acaatgtagt tgcaataggg ttgataaaca agataaagta ttgaatgcca
     9421 ctttcgattc cgagatggaa atcaactaaa taggcaaaaa tcgttttcaa ccaaagtaaa
     9481 acgactagta aagaaaaaaa tccgagcctc ttattcaaaa aggcaggtgt ttttatacat
     9541 ttcaagataa tcttcctttc tgcacttctt aatcacagaa gtgtcatgaa accatgttac
     9601 acagctcact tattctcatt tattttacta gtatgacaat atagtgtcaa ttgaaaccag
     9661 aagtttatgc tgtttcatta aggttttctt taagcttcat gaaaaaatga gcagtaagac
     9721 aagtcctatg ttttttgtta tacttgggaa gagaaagcta aagcaggtga aggaatgaaa
     9781 gtaaaagtaa aaaaaagagc aagcaatcga ttgaaaaaag gatatccact gatccaaaaa
     9841 gaagaccttg ctcatcccca aaaagaaacc acgcaatggg tggaattagt tgattcgcaa
     9901 aaccaattta tcgcaaaagg atatctagga aagcagaata agggaatcgg ttgggtattg
     9961 agccagcaga acatcccatt tgatcaatct ttttttgaag aattttttgt gaagtcaaaa
    10021 gaaaaaagaa ctttcttttt ttcagatgat tctactaccg cttttcgtat attcaatgga
    10081 gaaggagacg gaatcggcgg tctgattgtt gattattatg caggttatgc tgttttttct
    10141 tggtataacg aaacgttata tgcacatcgt gaattattga ttcaagcttt tcgtgaaatc
    10201 tacccggaaa tcgtaggagg ctatgagaaa attcgatttg aatcagtaaa tctgcctgag
    10261 agccagttca tttacggaaa agaggcaccg gaacccttga tcgtaaaaga aaacggagta
    10321 aaatttgccg tttatttaaa tgaaggatta atgactggga tctttttaga ccaaaaagaa
    10381 gtacggggat cattagcaga aggtttcgct tctgggaaaa cagttttgaa catgtttagt
    10441 tataccggcg ccttttctgt tgctgccgca atgggagggg caatcgaaac aacaagtgtc
    10501 gatctagcaa aacgcagttt aaaaaaaaca caggaacagt ttgaagtgaa tggtttggat
    10561 gtcgatcagc aaaaaatcat tgttatggac gtattcgagt atttcaaata cgccaaaaga
    10621 aaacaatttt tatatgacat gattgtattg gatccaccaa gttttgccag aaataagaaa
    10681 aaagtctttc gcgtagcaaa agattatggg gaactggtca aggatagtct ggatatttta
    10741 gcagaagacg ggatacttat tgcgtctaca aatgctgcga atgtctcgat agaacaattc
    10801 caaacaatga ttgaagagga attccatgcc aaaaatgttt cttttgaaca acttgcttta
    10861 tatcgtctgc caaaagattt ccagacgacg gatacctttt tagaaggcaa ttatctgaaa
    10921 gtgtttgttt atcaaattaa aaataaccat tagaaggagc gatgcagaat ggtttgggta
    10981 aagggattga atataggaag cggtagacca aagatttgtg cgcctcttat cggggccgtc
    11041 cgagaggaag tattacgtga agcagttctt gcacgtgaag caggggccga tttagtcgaa
    11101 tggcgagtcg atcattatcg tgaagttttt tcttttgatg aagtagcaga aacattggtt
    11161 cttatccatg agatgcttga tggactgccg ttattattta cgtttcgtac cttagctgaa
    11221 ggcggagaac aagacatatc gatccgtcag tatcgcgacc tttatgaaca tgtgatacac
    11281 acaggattag tagatatggt cgatatcgag ttgttcaaag tagaaagtct aggaaaaaga
    11341 ctgatagagg aaatcaagtc tttaaacatt cctttaatta tcagtagtca tgattttaaa
    11401 gaaacaccag cagaccctgt cttgctttac cgattgaatg ttatggaaca ttttggagcc
    11461 gatatcggaa aactggcagt aactcccaat aatgaacgag atgttttgcg gttgatggag
    11521 ttgactagac gggcaaatgc ttttgtctcc atgcctatta ttacgatgtc catgggaaat
    11581 cttggtaaaa tttctcgtct ggctggcgga acgacgggtt ccgttatgac atttggttcc
    11641 ttaagcagcg attctacttc tgctccagga caattaccgg tcaaagcatt gaagcaaatt
    11701 attcaattat taaatgaacc gttgtagaac aagtttgagg tgatattttt tggcaaagaa
    11761 aaaaatagta aaaacgaatg caatccgtat ggtcgaacaa aaaaagattc cctatacaga
    11821 gcacgaatat gagtgggacg aaagccacct aagtgcgtca tcggtcgctg aacagcttcc
    11881 agaaagccaa tctcgtatat ttaaaacact ggttgctgtg ggcaatgtga cggggcctct
    11941 tgttgctgtg ataccgggag aagccgaatt gaatctaaaa aaattagcaa aagtttcagg
    12001 gaataaaaaa gtagaaatgc tccatttaaa agatttggaa gcaacgaccg gatacatacg
    12061 tggtggctgc tcgcctatag gtatgaaaaa actatttcct acttatttag atcaaattgc
    12121 agaaagctat gaacaaatca ttgtctcggc tggcagaaga gggctacaga tggagttagc
    12181 acctcaagat atctgtgcat taacgagcgg acaatttgca gatataaaac aataaatcat
    12241 acgtataaaa gaaagaagga ggccgggatg ttgtttgtcc agtctccttc tttctttttt
    12301 atacattatg ctctttgccc ggcattcatt cctgctacat gccctgtagc gaaagccgct
    12361 gtgatattaa agccgccagt ataaccattg acatccagta attctcccgc aaaatacaga
    12421 ccatcaatga ttttactctc caatgtttta ggataaattt cctttagaga aacccctccg
    12481 ccagttacaa atgatttctc aataggaaaa gttttgttga ttgatagctt aaactctttg
    12541 catagttgaa caaaatcttg aatttgtttt tgagaggctt gttgtcctgt cagatgatcc
    12601 atttcaagcc gctcaagaaa gaaaactaac aatcgctcag gcaatatacc atgccaagca
    12661 ttgaccaagt tctttttggt tgctttactt ttttctgtca atatatgaat aagctcttgt
    12721 ttggtttgtg tagggaaaca atccagagaa agtgtcactg gttctcccgt tttctctaat
    12781 tctttattga caaaacttga acaacgcagt gcagccggac cagaaatacc gaaatgggtg
    12841 aaaagtaaat ccatcgtatg ttcagtcaat acacgacctt ttttatttaa aacgcgtaaa
    12901 gtgatgtctt gtaaagaaag cccttgaagt gttttctcct gaatatacgg ttcatccgat
    12961 atcaaggggg actctgtcgc ataaagaggc gtcaccgtgt gacccactcg cttcacgatc
    13021 ttgtacccat ctccagtaga accagtagat ggataagtcc gtccaccagt tgttaagata
    13081 atggcaggag catggaactc ttcgaattcc gtgcgtatac cgtaaatttt atgttctttg
    13141 tggatcaatt tctctactcg agtagagaat agaagcgtca catctagttc tttcagacga
    13201 ttgagcaatg cttcgataat cgtttttgat ttatttgtta caggaaacat ccgtccatga
    13261 tcctcttctt ttaaatgtac gccttgtgat tcaaagaagt tcatgatatc gaaattattc
    13321 cattgcgcaa acgtactata taaaaacttt ccatttccag gaatatgtgc aatcaaatca
    13381 tcaacaggac gattgttcgt aacattgcac cgaccaccac ctgtcatcag tagttttttt
    13441 cctgcttttt tgtttttttc gatcaagagt acgcgtgcgc cttgttcagc tgcactgatc
    13501 gctgccatca tcccgcttgt tccagcgccg actacgatca catcatacag tttttccatt
    13561 ccctcacctc aaggaaagtg tagcatatct gaagaaagaa aacagaaaaa aaatgttttg
    13621 agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga
    13681 caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc
    13741 aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca
    13801 ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta
    13861 ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc
    13921 gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg
    13981 tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg
    14041 gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata
    14101 atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat
    14161 cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg
    14221 gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt
    14281 tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac
    14341 atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg
    14401 ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat
    14461 gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag
    14521 aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta
    14581 tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat
    14641 gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca
    14701 aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg
    14761 aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg
    14821 ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc
    14881 agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg
    14941 gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag
    15001 caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt
    15061 ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc
    15121 tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg
    15181 tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac
    15241 gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg
    15301 acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag
    15361 tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct
    15421 tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct
    15481 ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc
    15541 gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt
    15601 tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta
    15661 tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt
    15721 gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg
    15781 gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg
    15841 atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag
    15901 caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac
    15961 caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag
    16021 accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac
    16081 aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc
    16141 gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag
    16201 gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag
    16261 acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt
    16321 atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc
    16381 cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac
    16441 caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc
    16501 tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt
    16561 taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt
    16621 cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga
    16681 ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca
    16741 gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag
    16801 gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat
    16861 gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt
    16921 ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc
    16981 ataagcatct acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat
    17041 atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt
    17101 agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa
    17161 ccctaaaatt tttttcatga aagatctcct tttttatttt tatta
//
LOCUS       DAZKAS010000048        16968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000048 DAZKAS010000000
VERSION     DAZKAS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.049"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8H7_001388"
     CDS             <1..1152
                     /locus_tag="ACG8H7_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFP8983490.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8H7_001389"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8H7_001389"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP8983491.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8H7_001390"
     CDS             2728..3003
                     /locus_tag="ACG8H7_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFP8983492.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8H7_001391"
     CDS             3009..3776
                     /locus_tag="ACG8H7_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFP8983493.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8H7_001392"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8H7_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFP8983494.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8H7_001393"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8H7_001393"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFP8983495.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8H7_001394"
     CDS             7920..8222
                     /locus_tag="ACG8H7_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFP8983496.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8H7_001395"
     CDS             8225..9388
                     /locus_tag="ACG8H7_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFP8983497.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8H7_001396"
     CDS             9414..12842
                     /locus_tag="ACG8H7_001396"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFP8983498.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8H7_001397"
     CDS             12863..13987
                     /locus_tag="ACG8H7_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFP8983499.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8H7_001398"
     CDS             13968..14414
                     /locus_tag="ACG8H7_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP8983500.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8H7_001399"
     CDS             14398..14736
                     /locus_tag="ACG8H7_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFP8983501.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8H7_001400"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8H7_001400"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFP8983502.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8H7_001401"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8H7_001401"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP8983503.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8H7_001402"
     CDS             15893..16936
                     /locus_tag="ACG8H7_001402"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFP8983504.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5749 a         2934 c         3747 g         4538 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacga
//
LOCUS       DAZKAS010000049        16896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000049 DAZKAS010000000
VERSION     DAZKAS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.050"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1672)
                     /locus_tag="ACG8H7_001403"
                     /pseudo
     CDS             complement(37..1672)
                     /locus_tag="ACG8H7_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2227..2790)
                     /locus_tag="ACG8H7_001404"
     CDS             complement(2227..2790)
                     /locus_tag="ACG8H7_001404"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP8983505.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2805..3848)
                     /locus_tag="ACG8H7_001405"
     CDS             complement(2805..3848)
                     /locus_tag="ACG8H7_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983506.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3981..4385)
                     /locus_tag="ACG8H7_001406"
     CDS             complement(3981..4385)
                     /locus_tag="ACG8H7_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP8983507.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4618..5253)
                     /locus_tag="ACG8H7_001407"
     CDS             complement(4618..5253)
                     /locus_tag="ACG8H7_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP8983508.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5519..6571)
                     /locus_tag="ACG8H7_001408"
     CDS             complement(5519..6571)
                     /locus_tag="ACG8H7_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8983509.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6584..7522)
                     /locus_tag="ACG8H7_001409"
     CDS             complement(6584..7522)
                     /locus_tag="ACG8H7_001409"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP8983510.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7973..8623)
                     /locus_tag="ACG8H7_001410"
     CDS             complement(7973..8623)
                     /locus_tag="ACG8H7_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983511.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8723..9232)
                     /locus_tag="ACG8H7_001411"
     CDS             complement(8723..9232)
                     /locus_tag="ACG8H7_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP8983512.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9433..10302)
                     /gene="scrK"
                     /locus_tag="ACG8H7_001412"
     CDS             complement(9433..10302)
                     /gene="scrK"
                     /locus_tag="ACG8H7_001412"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP8983513.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10322..11443)
                     /locus_tag="ACG8H7_001413"
     CDS             complement(10322..11443)
                     /locus_tag="ACG8H7_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP8983514.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11445..11840)
                     /locus_tag="ACG8H7_001414"
     CDS             complement(11445..11840)
                     /locus_tag="ACG8H7_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8983515.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(11859..12656)
                     /locus_tag="ACG8H7_001415"
     CDS             complement(11859..12656)
                     /locus_tag="ACG8H7_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8983516.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(12658..13446)
                     /locus_tag="ACG8H7_001416"
     CDS             complement(12658..13446)
                     /locus_tag="ACG8H7_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8983517.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13460..13936)
                     /locus_tag="ACG8H7_001417"
     CDS             complement(13460..13936)
                     /locus_tag="ACG8H7_001417"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8983518.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(14008..16665)
                     /locus_tag="ACG8H7_001418"
     CDS             complement(14008..16665)
                     /locus_tag="ACG8H7_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8983519.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(16729..16896)
                     /locus_tag="ACG8H7_001419"
                     /pseudo
     CDS             complement(16729..16896)
                     /locus_tag="ACG8H7_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
BASE COUNT         5360 a         3444 c         2507 g         5585 t
ORIGIN      
        1 ttattctcta gtaagctaat attgaaaaaa atgtaatcag atcattattt ttatatgttg
       61 taacttcctg ataagaaaac taattaaaag aaagaggtaa ctacataaac taattaaaaa
      121 agctaacttc atagttggag gataaatgga agaaacttca atgattgttt ctccttgtgt
      181 tccttcaatc gacactagtc ctattatcaa tctctctttt ttgtccattt acttttgcga
      241 ctgttcccca ataatcaaat acaggaagcg ttattttttg tcctttagat tcatgtactg
      301 tatagactat tttcgaatcc atcacttgat aaggcatagt atgccattcc ttgccaattc
      361 ttacttcatg attctgcaaa tgttctttgt attgtaatgc ttccgtgggt gcatagtcat
      421 aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt ttcttgattc attaccaagt
      481 gatctctctg agtgacaact ttatgttcat agtttgcttt tgttgcagca gatacagcca
      541 acagtaataa aacaattgcc gttatattaa ctacttttct ttgcttagct gatgtgctct
      601 tcatgaaaac gaacgataaa atcaacgagc caaacagtac ttgaaagcct aagattcgcc
      661 aaggaaattg aatcatttga atgggtgtat tttgtaataa agaccatgga aaaaatttgg
      721 tggataagag ccaagagact accgcactaa ggtaaatcac tttttctttg cgattaaatc
      781 ctttaaaaaa gaagagtgga cagatcaaag aaataatacc aatgattcct accgtaaagg
      841 ttgtcaggtc attattcatc gcaccaatca aactatccga gacatttaat gcttggtgtt
      901 gtaaggtcgt tttatctggc tcattaatcg attgaaccat gatttgtcgg aacatcggat
      961 accaaaagta tgctgttaaa agaatcgtca ttcctactgc ctttgacaac tccacaatca
     1021 accggatgct ttcttttttt cttgtttcac ttactacaaa gccgcctaag actagtaagc
     1081 caacggctat tacactcata aaagtggaaa gaacatgtgt ataaatgagt aagctcatac
     1141 tgactgctaa tggaattgca catggctttt tctcataaat cagtgcgtaa gcataataca
     1201 taactatcgg tataaccgta agtgcaatcg cttcagcaat agcagaacga tgataaatat
     1261 caatcgtacg gtacccataa aatgtatata aacagctaaa tataaaagcc gcatcgattc
     1321 tttttaaaaa ttttagtcca taatgaaaca tcagcatgat agtacaaaca ttcaatagcc
     1381 aaacatataa gatataacta acgattaaat tattacttat ccaataaaaa atgactgccg
     1441 gaaaaaaggt caagtaggga taaaaataat tcactccgct cccataatga ttaaacgtcg
     1501 taaaattgat tggaccagaa aaaattgaag ataacccttt gactctagca aaatggaagg
     1561 caatatcccc cgatgtagaa atagtatgaa catttctaaa ataattgatt gaataaagtc
     1621 cagcaataat tgctaggaga atggacaata ttattttttt ctttacttgc attacttaat
     1681 tatatactcc tttcatggat tgctttatct agtataactt aaattttaaa taaatcgtta
     1741 tttattttta ctctcactta aaaaagcact aaaattataa gagagggagg taatggtctg
     1801 aacaccaaga attgttccaa catcgaattg cttaactaat gagttcaaag cttaattatg
     1861 tggctcttat atttaccgag cgtggattgc gatctcttta gcgaaacaaa catctttcat
     1921 aaaaaaaaca gccatatccg ctttcttgga tatgactatt tactactact ttactatctt
     1981 tcattcttct tgtctcactt atgataaggc gctgcgtatc atgagtgaat gatattagga
     2041 aaacttcata aaataaatct tttatacatt tccacttcta gatggtcatt catgttagaa
     2101 gaatcaaact atttttttac tgtcgtaata atattaaaac actagaacaa atcaattaaa
     2161 ttccataaaa tataaataat agacctagca tcatctggta taaaatggat gctaggtccg
     2221 ttatatttac tcaaaattga ttctacgaat agatcttctt atcatttcta ttttagtagt
     2281 catgcgttgt tgttcttcta aaggtggtaa tggtataagt agtttagata aatttgtacc
     2341 atttacattt ggttgcccag ttccgtaaga aattttttct aataacttcc aatataatgg
     2401 cgagtctaag aagcagtcta cgtattctga taataatttc tcaactaacc taattcgaat
     2461 caagtaagaa gcaaaaacag attcctcact tatttcctta accaagaaac tttttcccat
     2521 agttcctcct gtccttgcta tcaaaatgtc attttcttcc aatcttaaat caactagttt
     2581 agaattacta atatcacagt aaggcacact ggaccaattt acacgtccct cttgtatatc
     2641 tgtgattctc aaaaacttaa cattgccttg ttttttcgcc gaatctgtat agccatattg
     2701 aatcttagta gaaatacttc ctaaataaac ccatagtcga acatcgctag atgatttttc
     2761 ttgtcgttct ttatgaacaa cagtgatcat cttgagttgt ccgactattt tttagttgca
     2821 gcaatctttt tgggacgctt atttgcatga agcaacgttg ctaagtctag taataaaaca
     2881 ttggctaaca caggattggc tttctcgcga tacgctgcgg cgccactttc aaacaatact
     2941 gctagacgat cttctacaac aacgatttgt tccaaatacg gtggaaattt gattcgtttt
     3001 aggatccggc taggagagtc ccgctcggac actaagagtt ccgaggtata aggaccataa
     3061 gaacgactgg ctaagagata atggccataa aaagccacac cttgaatctt atcagatgtt
     3121 tttctgctca ttgtcacggt tggttggatt ttgtctttgc ggcgaattgc tgttttcatt
     3181 tcagctatcg tcggcaatag atagaaagtc aactgtcctt caccttttag tgtgaagttg
     3241 ccagatacca gttgatcatt gttttgagtc aaatacgatg cttgtggaat ctctgagaga
     3301 tcagttgtca gccaataagc gattggctgt cccgtctctt cagatgtatc tgctaagata
     3361 tcgatcaaac gtaaagcggc tactcgtcca gtagcagatc ctgtgattgt caaccataaa
     3421 agttcccgtt ttgtatcata tactaagccc cctacatgtg gccgtttagg taaaatcagc
     3481 agtttgatcg gctgtcctgt ttctctatcc agtacatgaa taatagaatg atgctgatgg
     3541 tcatagcagt aggcagaaat aaacacaaaa tcttgagaaa aagccaaccc ttgcggcgtc
     3601 atccattgac aaacctcatt ttcgccggtt tccggcacca ccattcttgt ttctgttaat
     3661 ccaggaatgt tatattgttt tgcttcagcc ttttcccaag ctgcttgaac aataggatac
     3721 cgttttaact ttttttcaat atcctgatgt atccgttgct gcttccaatg cttaaacttt
     3781 aggacatagt aaacaagaag gccaatgacc agcaacacta aaatcattat tgctacccgc
     3841 tgtgtcattt ttttgcctcc ttgttctatt cgatcaatcg tccatatacc gctattaatt
     3901 accgtaacta catgtttctt ttactagcgt ttctttatag gatctttcat aatctcattt
     3961 atagtaacat taagaatccc ttattctact atagtcactt ccacatcaat attgcctctt
     4021 gttgctttag agtatgggca aaatgcgtgg gcttcttctg cgattttctc tgcttggtcc
     4081 ttctcaagtc ctggcaaaga aacttctaaa acgaccccga tttggtagcc cccatcttct
     4141 aattgaaata aagatacccg tgctttgact tcagaatcag actcaagatt attttcttcc
     4201 agcatgtgtt gaaccgcgcc gttgaagcaa gatgcgtatc cagccgcaaa aagttgttcc
     4261 ggatttgtcc cttcactatg gattcctggg cggtcgatct tcatgtgcat acttccgttt
     4321 ggtgcctcaa cttcgccatc acggcctcca cgattgatca ttgctgtttc gtacattttt
     4381 ttcattgaga tcatcctttc tttcgtcgtt ctatattaag tgtaacgagt attttttaat
     4441 ctgacaaagg taatgcttgc attcggcgac atcagttaga caacatcgat gatcagtgat
     4501 agaagcagtt tatctataca aaaaaagaag ggttctatga tatttggtgt ccttactcgc
     4561 cttgagtaac aacaccaaat atcatagaac cacttaatca gcttcacact gctttttcta
     4621 ctttcgtagt tgaaaaaaac ttaaacaaat catcggcaaa tagttaaaga aaaaacttaa
     4681 tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg gaatccgtgc
     4741 taatccgtaa agacctgcaa ttagtaaact aataactgtt aaaacaagat aaaaatggct
     4801 aagcgtacct tgaccacttt gacgataaag cagaacaagt aacaatggga aaagtagaaa
     4861 gcctgcatag cctagcccag aacctatatt atggatccaa gtcgatagat tacaagagct
     4921 ttcattcgta ttgatactga ataagccagt aaaaatacaa tcacctatcc catataaacc
     4981 aactgttgca acaacaagta ttgcaagtgt ttttgatgtt ccaacaaata ggtgatacaa
     5041 cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact gaccagacca agaaagcacg
     5101 tctgactgga ctatctacgt caccaaacac actaattacg gtagtcattt gattcagttt
     5161 gggataaaaa aggccaagga tatacggtgt cgataaatca ctcatcacac ctaataataa
     5221 aaaataaaaa ccatattttt gcaaaaaaat cattttcttc ccctactttt tagtgattat
     5281 tcgatacatc caacaatcaa attttttctc attttaacac atatttttat tctagttgtc
     5341 ttttgaacaa tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa tcgaatggca
     5401 ctgaagagtt cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa acgtaagtca
     5461 gatgttgttg acatctcact tacgcatcca ttgattgaaa atttcttagt aaaatctact
     5521 agcctaaaaa tttgaagact tcttctcgat catacagttt aaatactttc tcgcataaaa
     5581 attgagtaat tagtgccgca aaaacaggga tgactaacca acaaagaaca acaagaaagc
     5641 catttagccc tgcatctaaa gaagccaacg gaccaacaag tccgaccacg ccgaaaccag
     5701 cagaagcagg cgtacctgaa atagaaaata gagcgacagg aatagctgta atcactgcac
     5761 taactaaaca aggaataaga atcacaggtt ttctaaatag attaggcatc atcatcttca
     5821 tagcgcctaa agcaatcgca attgtcacac ctgacttatt aaccttccaa gagttcacta
     5881 ctaaaacaaa agtggttgca gctacgccca tcgcagccgc accagcagaa acaccatcta
     5941 gttggattgc cattccgatt gctactgttg aaataggaga gataatcaaa aaggcaaagg
     6001 aacaaccaat caagatagac attaacagcg gttgtaaggt agtgaagtta ttaatcaatg
     6061 atccaattgc agaagtaatt gctgttacaa aagggaataa ataaaaacca agtaaacctg
     6121 cacctacacc tacaacaatc ggtgttaaca caatcgacac tgacccaaaa ctatttccaa
     6181 tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat cgtattaata atatcgcctg
     6241 taccagcacc aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg actacaccag
     6301 aaccaacaaa agaagctccg gctacaacca tcattctcat cggatcaaat ccaaattgta
     6361 aggcaatcaa accaccaatg atcaaaggtg ttgctagttg aaaaataact gctaactgtg
     6421 cgatcgttac cgcaaaggga tacgcactaa aatattttaa aatggcacct aatactgcat
     6481 taggaatcaa tccaataatg attcctgttg ctgtaccagc gaggacttta ttcagaaaaa
     6541 ttttcggtga tattttttgt gaagtttcca ttcaatctct cctttatttt acgttcaaac
     6601 tatcttcttt tgcatggaca agctcagtca agaaatcaca ataaggcgtc gcaacaccat
     6661 atttcttccc ttttctagaa attgcgccat tgatataatc gatttctgtt aatcgatggt
     6721 tcttaatcaa gtcttgatac attgaaggat aatgtaaccc aattgtttcc gggtcaaaac
     6781 aagattcaca atgtgcaatg acttctggga catctagttc aatcttctct actgctgcta
     6841 ctttggcaaa ctcattgaca atcgtcgcca ccattttatg agcagtcgat gtttttccta
     6901 gctcagccat attaacgtct aagattgtgc atagtccgtt cattgttccg ttcacacatg
     6961 ctttgcgata aatagaatag tgaatattgt cagaaaaatg agcatttaat cctgattcag
     7021 acaatttgtc tgctaatttt tttgcagctg cttcttttcc atcacctaaa ttttgtaatt
     7081 ctactgaacc acttccaaat aatttgacct gaccaggacc ttcaagacct gccgtccaca
     7141 tggtatttcc aatatagata ttttccattg gtacaaattt ctcaataata tcttcatgcc
     7201 cgataccatt taatagacat aagacctctg tatctttttt gattaatgat tggatatcct
     7261 gcagcatttt ttccagctgc atcgctttgg taaatagaat aatcagatca acttgatctt
     7321 ctttttctac ttcggattga agaacgattg gtagttttgc ttctacctct ttaccattaa
     7381 aatttgcttg taatccatgt tctttgattt gttggacatg ttctgcccag ccatcgatta
     7441 ataatacttc gttcccactt tggtgaagca tcaacccaaa gcgacttccc attgcgccag
     7501 cgcctgcaat tgctattttc attgattctt cctccttttt ctttctatta tactcatttg
     7561 agaatctttt cacaacctcg ttttgttatt cttctcttat ttatcatgtt tatatccacc
     7621 attcaagtta ttttatcagc aaatcaataa aaactggaat agaatcccaa tttcatcgta
     7681 agagaaacta gactagcaat aagaaaatag aaacattcat agaaaaaatc tcactcctct
     7741 gattataaaa gagggggcat taacctcgtg tatactaaaa ccttccattc attcgatgga
     7801 tgattttctt tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac acagacggat
     7861 ggtattgctt attatttatt aaaatcgaac aatacactta tttaaaaact tctttcccag
     7921 attttcttcc atttttacag ttataactag tattatcatg aaaaatagcc ttttattgtc
     7981 tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg cccaataatc
     8041 tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag tctaaataat
     8101 tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc cttaaaatta
     8161 tattttcagt gccagacaag tgatttatat tgagttgata aaaaagatct ccatctaaaa
     8221 aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct ctatgtgtta
     8281 catacaaata attgtatgac ctcatttcac ctaatgaatc aattatttct tgccctttag
     8341 ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt tttttcatgt
     8401 caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc caatccgaga
     8461 ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac tttttttgtt
     8521 gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc tctatctctt
     8581 caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca atccagctcc
     8641 tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa caaccattgt
     8701 tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct aaccccttga
     8761 gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct tcttttctca
     8821 aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa acgtctaaaa
     8881 tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca ttgttgatat
     8941 atttgatttc cttgcttttt tgcggcgtgt ctactatcgg cttatctttt tgcggaccac
     9001 gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg atggtttttt
     9061 catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc agtaccgcct
     9121 gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc aaatccgtat
     9181 ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc atttgttcct
     9241 ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat ttttaggatc
     9301 aatgataaaa accgcatcac ttatgataag gcgctgggta tcatgagtga atgatattgc
     9361 tgaatacaaa tagtccgacc acattaagtt gatcggactt cataagcatt ttattgacta
     9421 atactttttt gcttaagaat tgattctaac atcttttgct aaagttaagc atcctatagt
     9481 tcctgcatta tctgctaact taggtgtgac aatgtattct tcgatatttg gcgtatcgac
     9541 atagccgtta attagttcta aaaaacgtct ttgtactttc tttttcagat gttcttgttg
     9601 catgacacca ccaccaaaaa ttataatatc tggtgaaagc atcagggtcg tattacaggc
     9661 acattgtgca atataatccg cctcgatttc ccagaacgga tcttctatag aaagatcttg
     9721 tcccttggtt cccgtacgca tctcgatagc gggtccagat gccaaccctt cgagacaatc
     9781 atgatgaaaa ggacaagtac cagagaaagt atctcctgaa tgtctcttaa ctaacgtatg
     9841 ccccatttct ggatggctaa acccttcaac aaaaatccca tcttgtatag ctcctccacc
     9901 aattccagtt ccaacagtgt aataaacaac actggataaa ccttttccat gacctgaaac
     9961 atactccccg taacaagcag cattcacatc ggttgtccag gcaattggaa tttcgaaatg
    10021 cttattcaac gttccaacaa aatcaaaatt tttccaggca gtttttggag tagaggtgat
    10081 atgtccatag gtctttgatt cacgatggat atcaatcggt ccaaatgacc ctactccaat
    10141 gctttctaat tgttctttat agtgattaaa gaaatcaatt accaaactca ttgtttcatt
    10201 cggtgtagtt gtaggaaaac tcactctttc taaaaccttt aaatcatcat tgccaatggc
    10261 acaaacaaat ttagtcccac ctgcttctat tgagccatac atttcttact ccaccgtcct
    10321 tttatctagg aattcgttct tccctgtcta atagtttttc gaaaaactca tgtggttcct
    10381 tttggtacca ataggcaaca gaagaaatat cgtcttgacg ctcataaagc ttgatgtcat
    10441 catttcctag ggtttggaaa gtaacacgta aattttcttt gaaactaatg gggtcgggta
    10501 aatgccagcg gtacaaccca tgcatcggta aagcatcatc gccaaaacca tgaactggat
    10561 ttggattagg ttttccagca ctaaaataat cccgggtgtg atctctttta gattgaaaag
    10621 gatagcccat aaatagcgta ttaaaatttt gagcatatgc cctttctcca aagttctttt
    10681 tatggaaagc ccaagcgccg ccaaaataat cttctgctcc tgtagatgtt attgtaggga
    10741 aatcttcatc tccatctata taaaacttaa attctccttc cccccaccag tatctttcca
    10801 atgcgcacaa agcaaagtag gttcctacat aatacccagt gcctttgatt ccatcaataa
    10861 gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt ccatttagca tgaaaataca
    10921 tcgcatcatc cggtacttca gtcaatgcat aattaaatgt ataaaaaatg tgcttaataa
    10981 actcaggatg ttcattcgtt aaagtaatct tagctttttt tcgaaatggc atctcaaaat
    11041 agcaattcat ccctgatgtt ggattcacta cgattggtaa ggaattcact tcgcatcttg
    11101 tcccaaaccc attacaaaaa aaatctccta atggactttc tacagaagga gtggattcat
    11161 catcccaata aatacgcaaa attaaatcac gtaagacgaa actcccagcg attgttttgt
    11221 ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc ttccatttct gctaaagtaa
    11281 ttgtctctcc agcatttatc ggaacagaag gtgacccttt tctcccaatt cctagatgac
    11341 tcgctgtcat tgccgctttc cctttttcac catttttatt ttctggtgaa atagttctac
    11401 tttcctctcc cttgtggagc catatattat ttaaacaatt cattttattc gtcctctatc
    11461 ctttcttcac ttttttgatt attcatatag atgatttgtt gttgtgcctc cgcaataatt
    11521 gctccaatat ctaaattatc atttcctaac tgcaaagcga ttgacattgc caagggaaga
    11581 ttcattcctg caattacgtg cactctgtca gaaatatagg taaaaaactt ttgatttaca
    11641 cttcctccag ccatgtcagt taaaacaatt atttcgtctg tagtatccac taactcttta
    11701 atttgttcat ctatatcatc aatttcttcg acatatgcac ataagctact aatttgatta
    11761 tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct cagctagtat tgaatggcta
    11821 gcaatgatca atcttctcat aattcaaatc cttttccttt acgctaatat tccaaatgca
    11881 gccataacca tcgcaaaaac aataacgagt agaatcaaaa tggtcatact gatttttttc
    11941 ttactgagta gataatatgc tgcagcagta ataattactg gaattaatgt tggtaaaatt
    12001 ttgtccaaca ttggttggat tttcatacta acttcaccaa aagtgaattt aagtggcgtt
    12061 gaaacactga taactgaagc aattaatgat ccaactactg ttagccccaa tactgaagca
    12121 gcttcagtta aataattaat cttttcaccg tattcagtaa caatcttcat tccctgctta
    12181 taaccaaatt caaagcctct cattcggaaa aagaagatta ccatgattaa aatgaaccat
    12241 aaaaagattc ctacaggatt attttctaaa gccatataag ccgcaattga tcccatgatg
    12301 gttgggatca tcgtcatcaa aagagagtcc ccaataccag caattggacc catcgtacca
    12361 actttaaagt cattaatcgc atcttttgct tctaatcctt gcttttcctc cattgcaaca
    12421 caagcaccta aaataatggc tgccagccaa ggctgtgtat tgtaatactg aaagtgatta
    12481 tttaaagctt cgatgtattc atcatcatct ttgtataatt ttcttaaaat aggtgacaat
    12541 gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa aggtacaaac tgacatcaac
    12601 caacgatagt tcgctttcct aagatccttt ttcgttactt gatatttttc gctcatttta
    12661 ttcatcctcc ccaataaacc caacatcaga agctcccgca gaagcagtga tttgtgcatc
    12721 tttttcagct ctatgataca cattaaatgc taaagcgaat cctatgatcg ccgctccaat
    12781 aataggtacc ttcaaaaaag cggctaagaa aaatccaaca agtaaataac taatgtatcg
    12841 acctgtaggc ataaagcgta acaacatcgc aataccaaca gcaggcaaca tttttccggc
    12901 taatgttagc ccatccataa accaattagg taaaacatcc aaaacgtaat taacagcaga
    12961 ctgtcctaaa gtaacggcaa taaatactgg aattgctgca gataaaccaa agaaaattgg
    13021 ccccaaccac aaaacactat tcattttctt aaattctcgt ttattcgcat attcctgtga
    13081 tttgcgaaca acaaaaccat ttaagatttt gacaattaca tctagttgca cccctaacat
    13141 acctacaggt agtcctattg ctaatccggc ttctactcca agctggctac caaatgccgt
    13201 tgcaataatt gcgcacgttc catagtccgg tacagatgag ccaccaaaac cggcaactcc
    13261 taatgacatc aattgaactg taccgccaac tatcaaacct gtttttatat cacccatcaa
    13321 aattcctgtg aataatcccc aaaaaactgc gtaatttaca aagatcatcg gaatcccata
    13381 ataatcaaca tgtttaataa atgctaatat agtcagcata ataatttgaa aaatagtaaa
    13441 atgcattatc cttactcctt tatcctaatt ttgattgaat ttcctgtgcc ttatccgccg
    13501 gaacaaattg tgtaacaatt tctacaccag aatccaacaa ttttttatac gtgctgatat
    13561 tttcatctga tgcataggct ctagaagata gctgtaatgc attatcttta acaacagtac
    13621 cgccaacaat tagtcggtca attctaatac cgttatctac taaatgaagg attgtttccg
    13681 gctctttcac gatgacaaat actttttcct gatcgtattt ccccttttta aaattcgcaa
    13741 tcgctgtttc ttcagtgata attgatacag ccattccagc aggtctagct aaacgcatac
    13801 ttgatttttg gatgtcgtta tttgcgacag aatcatcgat caccatcact ctgtttgctt
    13861 tagacattgg tgcccattgc gtgacaataa tcccatgcaa taaacggtta tcaactctcg
    13921 acataacaat actcataaat acttcctcct ttatctttca ttttcttaca agcaattacc
    13981 atgccaaaac aaaaagaact tgataaatca agctcttttt acataatcat aaacgtattt
    14041 aacctcatga atcgggatgt cgacactata ataatgttca acgacactga aagattgctt
    14101 aaaatgtgtc tgaaattttt gaaattcctc actcgtttca ttgaaataaa tttcatcttc
    14161 caaagtattt tgttttacta atcgttcaat taagcaacta atgtgaacat ataatccgaa
    14221 acacgtatta ttatcaaatg aaattcctag atcattttgt aagatgtata tagcatctga
    14281 aacccgttct aataatttag tcggatttaa tatggtcaat tgctccatta aattcgttag
    14341 actaaaattc ttcagaatat tattgttcaa aactttcagt tcattatcat taaagtgtct
    14401 attcaattga ttggagaatt tacttatatt cattcctaaa atcatatctt caattgaaaa
    14461 gaattctaca tcttctagat gtggatttag tgttccaaca ataaaaagaa tattgttctc
    14521 tttacgaagc tccgataaat agttttcatt cactaaagag taatagtcac acgtttctat
    14581 gacaagatca gtatcccttg gaaagctatc ttcaagtatc ttttttaatt taaacgaagt
    14641 tcctaatccg cttgcacaag aacagacaat catatccttc ttaatatttg atgattcata
    14701 aatataatcc acattttgat tttcttttac ttgttcaaga atctgtttaa gcggtaaatc
    14761 taataatatc tgatttgcaa catcaatagc cattcttgtt gaaacattgt ttagtagtcc
    14821 gaattttttt tgagcgtcta gttccaaatt tagataaatt ttctccagtg aacccatatc
    14881 aacaagtaaa ataatttctt catatccggt acactcttgt aagtatctat taattttgtc
    14941 tgcaatctca ctacttgaag tcattagtgg catatcaatg gcgtcgtata tatatctctc
    15001 aacgatccta ttagcagtac ttgctataca ggttgccgtt agatctccat gagccaaaat
    15061 cagtgctaaa cgtttttgct gaaattcttt actacacatt aacttaaata aaataaccaa
    15121 aacatttttt tgaatttttg ttaaataaat atcccctatt tgaataaaac gattgactaa
    15181 ttgaacttct cgactacacc aactattaat atcattgatg aaggaatata atttatcgtc
    15241 cgatgttaat tggatttgat aaatccaaga attcaaaaag atagacgtta tttgaagata
    15301 ataatagtca aattctattt gaaaatctct ctctatttcc ttaacatact gtctaaatcg
    15361 aatctcttga taggtgggat atgttaaagc tgaattattt tggatatcaa cattcttttg
    15421 tctaaaatat ctatcatatt tctcaaaaag ctgctgctta tcagtgtctt tcttccacgt
    15481 ttgataaaga tactctataa gttcgttgat attaaaaact tcgtctactt tttccaaatc
    15541 atccatttga atatatttta attcattctg tcttttcata accttcatgc cgttgaaatt
    15601 cacccattgt ggtaaatgat acatctgaat ataaagttct tccaaatcat ctaagaaata
    15661 aaaggcattg gcacaggtca attgaatgat atttttcaaa ttgccaacat tttcactaaa
    15721 gcgatagttt aataaaaaat ctaataattg ttgagatatc agtattttct ttgctagtct
    15781 ttcttcttct gatttaaaaa atatcttgat taactgtttt ctttcataaa gagaacgact
    15841 gtctaatgtt ggaagtgtta gctttacagg tattcttctc aacaaagttg ataatagttt
    15901 atcctcaggt ggttctgatg ttgcaaatat aatccagcaa cttgatgtgt accagttttc
    15961 attatcaccc atccgatgaa atttcctcgt atccataaac tgaaaaagtt tctcctgaca
    16021 ttcattgctc aatgcatgaa tttcatctaa aaataagact ccattattag ctaattctgc
    16081 taaacccttt cttccatctt cggctcctgt atatgcacct tttacatgcc caaaaaaatt
    16141 ggacaagaaa agttctggat tattggcata ttcactacaa ttaactgaaa tgaaagatgc
    16201 cttctcttct atataattat tggccaacaa atccttatac acataatttg ctaaatagct
    16261 ttttccagtt ccagtctcgc catgaagtaa aatgggtaag ccatttggcg gatagtagat
    16321 tgctgcttta atttgagcaa ttacatttcg cagacttcct tgacttccga taaccctttc
    16381 tattccaacc ctaaaagagc tatcatacca tttttccgct tcctctgaag agctaaaaat
    16441 ttgttgattt ggaactttct ccggtaaaga aaagaagatt actggacgag tattgatttt
    16501 tccaaattct ccatctttta cgaattcatt caaatattga gagacaatat tacgactaac
    16561 atttaatttt tcagatattt tctgtgctgt attgttcccc catttatttt taatctcaga
    16621 gttctctatg acataattga caatgcgttc ttttaattct tccatcacag cacctcctcg
    16681 acactattcg aattttgccg acagtatttt tccataaata tgacttgcta aaaaggcttt
    16741 gtttagatca ttcaattgga acgttgcttg ttttgcttga tccgctaaga cgttgaaaag
    16801 atactctggc gaaatcgcat accccaaaat atcaacgatt gttgcaatca gatcttcttt
    16861 tgattcttca tctgaaagta attcacgata caacat
//
LOCUS       DAZKAS010000050        16858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000050 DAZKAS010000000
VERSION     DAZKAS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.051"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(369..3074)
                     /locus_tag="ACG8H7_001420"
     CDS             complement(369..3074)
                     /locus_tag="ACG8H7_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFP8983520.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3380..4327)
                     /locus_tag="ACG8H7_001421"
     CDS             complement(3380..4327)
                     /locus_tag="ACG8H7_001421"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFP8983521.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4517..4786
                     /gene="rpsN"
                     /locus_tag="ACG8H7_001422"
     CDS             4517..4786
                     /gene="rpsN"
                     /locus_tag="ACG8H7_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP8983522.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4962..5324)
                     /locus_tag="ACG8H7_001423"
     CDS             complement(4962..5324)
                     /locus_tag="ACG8H7_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP8983523.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5335..6456)
                     /gene="alr"
                     /locus_tag="ACG8H7_001424"
     CDS             complement(5335..6456)
                     /gene="alr"
                     /locus_tag="ACG8H7_001424"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP8983524.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6472..6822)
                     /gene="acpS"
                     /locus_tag="ACG8H7_001425"
     CDS             complement(6472..6822)
                     /gene="acpS"
                     /locus_tag="ACG8H7_001425"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP8983525.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6952..8463)
                     /locus_tag="ACG8H7_001426"
     CDS             complement(6952..8463)
                     /locus_tag="ACG8H7_001426"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP8983526.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8785..10137)
                     /gene="murF"
                     /locus_tag="ACG8H7_001427"
     CDS             complement(8785..10137)
                     /gene="murF"
                     /locus_tag="ACG8H7_001427"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP8983527.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10290..11366)
                     /locus_tag="ACG8H7_001428"
     CDS             complement(10290..11366)
                     /locus_tag="ACG8H7_001428"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP8983528.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11562..13307
                     /locus_tag="ACG8H7_001429"
     CDS             11562..13307
                     /locus_tag="ACG8H7_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFP8983529.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13587..14024)
                     /locus_tag="ACG8H7_001430"
     CDS             complement(13587..14024)
                     /locus_tag="ACG8H7_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP8983530.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14008..15381)
                     /locus_tag="ACG8H7_001431"
     CDS             complement(14008..15381)
                     /locus_tag="ACG8H7_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983531.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15703..16191)
                     /locus_tag="ACG8H7_001432"
     CDS             complement(15703..16191)
                     /locus_tag="ACG8H7_001432"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8983532.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16213..16815)
                     /locus_tag="ACG8H7_001433"
     CDS             complement(16213..16815)
                     /locus_tag="ACG8H7_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP8983533.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4735 a         3610 c         3065 g         5448 t
ORIGIN      
        1 tgacttagaa aatcaagttc tatccttttt gatatcttaa cttttggaag agagatgtaa
       61 ggctgctttc tacccatcat tcttactact cgaagcatag cgcagttgtc acaacctctt
      121 gataaacggt gtttaaaagc ttgcatctgc ggtaataagc ccaaccaaat taaaaacata
      181 agaagctgtt tttatttggt cgttcttatt acttgatgca gagcagcttt tttacaacct
      241 cttggtacac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaaa cggcaaaata
      301 tgaggaacta tttttccatt tttttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttttt aatgaaaaat atggctctca tgcgccgctt ctttgaattt cgtcattttt
      421 tcttcgatga acggatagat tgccgttcct acgattccaa agacaacaaa caaaccgatc
      481 atgatccaga ttgctttcgt agcatttggc caataaattc ctcctgcaga ttcccgtaac
      541 aagtttaccg catgagtaaa tggcaagaac ggattgatca tttggaagaa tttccctgag
      601 acttggatcg gatagttccc gcctccgcca gagatcgaca gtactaagat gataatagcg
      661 atccctttac cgacattatc gaataatgca gccaagacat agacgatcat catgaacgct
      721 aaggcgatca agacagcaaa tgacacgctg tagaccggat tttggacatc tactcctagt
      781 aagaaaatat tccctaatgt aacgatcaag ctttgcgcaa tcgccattgt caaaaatgtc
      841 agcatacgag caacgaattg ttctcgtttc gtaaaattgt ttcggtcttt ctttcctaaa
      901 tagtattctg tcgtagcaac acttgaaaga agaacagcgc cgacccacag acatagtgca
      961 gtataaaacg gggtgctagc tgaaccgtta tttgcgattg gataaagcgc attggtttgt
     1021 agctcaacag gttgtgtaaa gaagtcactt tcttttgttg cgtctagttt caataatttg
     1081 atgacttcgc ctagatcgac ttcttgttcg ccttttcgga tcgcgtctgc tgctttttgg
     1141 ataccagctt tgatatttgg ccagtcattg ttgatcaagt ccgcagcagc gttcacagct
     1201 gtttcgactt ctggcatttt ttcatttact gttgtcaatg taccggtgat ttcatttttt
     1261 agtgctggcc aatcattttg gataaaatca gcggccaatc ctaatttttg ctgaatgatc
     1321 ggtaattcat tgttgtatag gtctgcccct tcattgatgc cattgacgat ggtatccata
     1381 tttccattca gcattgtatt ggcatcatgg atttcttgtc caattgcagg catttctttt
     1441 tgatattttt ctaaaattgc cactgcattc gttacagttg ctttcgttga gctgagaagc
     1501 gaatcaaagt cgatctggtc cgctttgctc agtgcgcctt gtgcagtccc aatcgtatcg
     1561 atgacttggt ctagtagaga agtgacagtg gaactgattt gacctgtatt gataccagca
     1621 atgatgccat tgatattatt aacaacggca tcaatttgat ctagcaattc atcaatagcc
     1681 ttcgtgtctt cattttgaac agctgtgttc aaagcagata ggcggttttg cagattgctt
     1741 agcaagggtt ttaattgatt tagctgatca ataattggct gtaatttcgt atttccagca
     1801 cttgtttgca atttagtcag gaactcgacc agtttatcga ttgcattttg ttgttgagat
     1861 attccgtcaa taaagccgtt caatacattg ctgatgtttt tcttttcatc atcagtcagc
     1921 ttaccatctt caatagctgt tcttacagta tctattaacg aacctgtcgt tgttgccact
     1981 tgtccaattg acttcaatgt aacatctaca ctgtttgtga tgcttggtat cgcatctttc
     2041 agctgagagg cacctgattt tgtttggttt gctagatcac ttgcctgatt ttctaaattt
     2101 ttcacatctg gcaagatatc ttgtacttgt tggatgatcg tcaagccttg ttttgcttca
     2161 tcgattcctt ggttcattgt ctcttggatc gaagcaaaat cgccgtcgat ctctgctagt
     2221 tgcttaccgg cattttggat ctctgggatt ttttcttgca aatcaaggat gatttttgct
     2281 tgatctttca actcaggcat tttgtcattc aatgcaacga ctttttcacc catctcatct
     2341 actttaggga tgtaatccac gaattcattt gctttattca gtttttcctt gatttcaggt
     2401 agttgacttt gtaattcgag gacttgtttt gtgtagccgt cgatcgtgtc caaattttca
     2461 tctgttgata aaatcatatc tttaacttta ttgatactta ctaggttcgt atcgatgtcg
     2521 taacctatct cattgaatac tttcaataaa gtcgaactgg ctgttttgat aaattcattt
     2581 gtgatttgcg attggatcga acttgctcct ttatcggtga ttttcggtgc aatcgcattg
     2641 attttttcat ttaccgtata ttcgattttt ggtttcttga tatcaccact agtaaaactc
     2701 agcagatctt ctgagaaatc ctttggcaag taaatacctg cgtaatactt tccggaacgg
     2761 actccgtctt ctaattgttc ttttgaatca acgaattgcc atcctaattg tttattttta
     2821 tgaagcgatt cgatgacttg ctccccaatg gccacttctt taccttgaaa ctctgctggc
     2881 ttgtctgcac tgtaaacagc aattggcagc tctccagtat ttccatatgg atcccataat
     2941 gctttgatgt taaaccacgc atatagcgaa gggatgatca tgatcgcgat gatcagaagt
     3001 gttgcaatcg ggtttttgaa aatccgtttc cagtcaagca aatataatcg aagcgtattt
     3061 ttgattgact gcatagttct cccccttctt cttttttcag ctgaattact ttaccatgta
     3121 tttcatactt ttttcaatcg gcttttttca aaaaagtcga atttttggac atatcacggc
     3181 aattgtctca ttgtaatctc ttacatattg agaaataatt aaggattaca gcctttactt
     3241 tatcatgaaa taaaagggaa agataatttt atgccaaagt ttttatctat tatacgtata
     3301 cgcaaaaaag ctaaaacatt ttttgtcttt gcccctacaa aaatgtttca gcttttttcg
     3361 ttttttattc ctgtatttct tatttttcga atgacaggat gattttccca gcaggaggat
     3421 gtccttcaac tagctgatgt gcttggatga catgcttaag tgaagcagac agattcattg
     3481 aaataaaaac atgaaaggct cctttttggt aggctttgat tatgccagca aaggcttctg
     3541 catctagata ttcctttctt ggaacaaacg attcataaaa gccttccttt ttttgccgca
     3601 aatctagatc tgggaggtca tttaatgcaa catacctgcc gcctggtttc atgatctgga
     3661 tgccggtttc tcctttgata cttcctttcg tcgcatcgat tacaaggtct gcctgatcag
     3721 caaattgcag acctggattt gttttatcat aagcagcaaa tgctgaagct cctaattttt
     3781 ttaccttttc ctcattttta ctggatgcac tggttaagat tcggattccc ttttcatgga
     3841 gaagctggat cagacttgac ccgaccgcac ctgatgctcc tagtatcatt acagtatctg
     3901 tcggctggat ttccgtcaaa tggttgatta gattataagc cgtaattcct ggcgtgacca
     3961 ttccggctgc ttcttcccaa ctcattttat ctggaatttt tgctactttt gctgatggca
     4021 acacgacttc ttctccgtaa gtcccgccga ctgcatggac agctactcga tcacctacat
     4081 gaacagttgt gacatctgaa ccaatctctg tcacaatacc agcgccatca ttacctggta
     4141 cataagggaa tttcaatgtt cgtatctctt tcatttctcc taaacgcaag gcaacatcat
     4201 aaggattcac actgaatgct tttatttcta cacgaacatg tccagaaccg acttctctgg
     4261 gatgagcatc gatttcttca aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc
     4321 ttcgcatggt caccatctcc ctttttcttt aagtttacgt ttatgccttt attttgacaa
     4381 aaaaaatgtc ttttcgtttt ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt
     4441 aacgtgttaa attatttatc taaaagcgta aagattacga tttacgctaa taaaaaataa
     4501 ttggaggaat ttgttgatgg ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga
     4561 aacgattgaa cgctatgctg ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg
     4621 cttgcgtaaa cttcctttag atgctcgtcc agaacgattg aaaaatcgtg atttaatcga
     4681 tggacgccct cgcggataca tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt
     4741 agcacataaa ggtttgattc caggagtaaa aaaagccagc tggtaatcca agtctggctg
     4801 taatgtaaag gaggagggcg catggaccac tccttggatc atcaaaaaag atacgttcgc
     4861 ttcttctagc agaaatgctg gtactagaag cagacgtatc tttttttacg cacagctggt
     4921 ccttttttat atgtaaaggc tgcatggtta cttcgctata tttatttagt aacaggagtc
     4981 agtgcttcga tacccacact aattttcaat gctcggtcaa cttctgccat aatgggtcga
     5041 tccaaatggc agaccttttc tttcaatctt gatttatcga tcgttctgat ctgctccaac
     5101 aggataacgg agtctctttc tattcctgtt ccagtagctt tgatcccaat gtgagtcgga
     5161 agcttaggtt tagccatttt tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg
     5221 ttacctagat tattttgaat aaccaaaact gggcgtgttc cgccttgctc cgatcccacg
     5281 actggagaaa gatcagcaaa ataaatatct cctcttttta ccataggatc ctccttattc
     5341 ttgatattct cttggtatcc gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc
     5401 taattgatct gctatatcct gcatagtgat ttctttgtct gcatttttgc cgataagcgt
     5461 cacttttgtt ccgacaggaa attcttgagg caatcggatc atcagttgat ccatacatac
     5521 acgcccaatc gtttcacagt agttcccttc taccaataga gaaaaaccct gcatctttcg
     5581 tggccagcca tctgcataac cgatcggtat cgttccgatc cactctgctt caggagtgat
     5641 atatgtttct ccatagccga ttccttcacc tgctgggagt tttttgactt gaatcaattc
     5701 agaaaccagc tcaagtgctg gctgcaatgg ataaacttcc ggtagtgcgt gcccagaggg
     5761 attaagtccg tacattgcta ctccataacg aatcatattg ccgattgttt catcatgcca
     5821 taaagccgtt gcactattgc tgacatgtac atatcgagga agttcgggaa gttttttcag
     5881 cacttctata aagcgttctt tttgcagatt ccaatatgta tcatctgcct gatctgctgt
     5941 agaaaaatgg gtaaataaac cttcccattc taatacacga ctttctttga tgaatgcagc
     6001 tgcctctttt acttcttcag gtgtacaaaa tccgatacga cccatcccag tgtctgcttt
     6061 tagatgaatc gaaagaggcg tctctagctt acttttctct aaaacagcag ctgctgcttg
     6121 caaccattcc cgtgtacctg cagttactga taaatcatga gcaacagcta cagtcagtgc
     6181 gtcgtaagga accatattca agataaggat gggctgagta aatccagctt ctctcaactc
     6241 gactccttca tctaaatttg aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc
     6301 agctgtttca atagctccat gaccgtatcc gttcgctttt actactgcaa acaattcttt
     6361 tccttgcgga agtcttgcaa cttcatttgc tacattttca gttatagctt ttttatgtat
     6421 cactgctttc gttgggcggt gccaagcgac aaccatcgtt tttccttctt tctagtcttc
     6481 caaaataatt tgtgcaaaag ctgtttcatt tgtatgagta atactgaccc atacattgcc
     6541 ttcatgaggt gaacgcgtca caacaggctg accattttca ttttttaaaa tctcgacatc
     6601 ttgaaaggta acttttccga tcccagttcc ccaagcttta gagaaagcct ctttgcaagc
     6661 gtaacgcccg cctaaaaatt ctacttggcg atgaaaaggc aaggactgga atagtttcat
     6721 ttcatcagag gtcaagattc gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat
     6781 cctaggaagt tcgactgcat cgatcccaat tccttttatc atatgctttt ctcttttcaa
     6841 taaatttaga acactctcat tctaccaaat tataaccatt ttgccacaaa taagtccaca
     6901 aataacaaaa atgaaagttt ctcttaaaaa agagaaactt tcattttatg tttaatcgtt
     6961 atttgaacgg attacaaatc cgcgtttttt atcattgtga cgtttaccgc catctttttg
     7021 gctgttatta tttttgctat aaccgccttt tgatttgttt ccacgataat taccgccgtc
     7081 acgacgatta cggttgtttc cgccaccacg tttgccattt ttattgtatc cttttttctg
     7141 cattggcaat gggcgttctg gtgtgatctt cactggcact gcatcagctg gatcttttgc
     7201 tgtcgttttt agcaaaagtg caactaaatc ttgtgcagaa tattcttcta ataatttatc
     7261 tgctgtttgc aggtatttat ctagaccatt ttctgcaagt tttgtttcga tttgttcaac
     7321 tgcagcacct aattgacctt tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat
     7381 ccgttttttc gtcaagtttt caatcacatg caagtagtcc atttcatttg gtgtaacgaa
     7441 agtcacagac ataccgcctt ttccggcacg gcctgtacgt ccgatacggt gaacgtagct
     7501 ttctggatct tgagggatat catagttgta cacatgagtg acacctgaaa tatctaatcc
     7561 acgtgcagca acgtctgtcg ctacaaggat atctaaatga ccgctcttaa atgaacgcaa
     7621 tacgctcatt cgtttttgtt gagacaaatc gccatggatt ccttctgcac ggtaaccgcg
     7681 tgcttctaat ccgcgtgcca attcgtctac acggcgtttt gtacgtccga agacgattgt
     7741 taattcaggt gtttgaacat caaacaagcg agtcatgata tcgaattttt cgtattcttt
     7801 tgcgcggaca tagtattgat cgatcaagtc agctgtcatt tctttcgctt tgatcttcac
     7861 atgatgtgga tttttcatga atttaacgcc aatattcttg atagctggcg gcatcgttgc
     7921 tgagaaaagt aatgtttgtc tttgatcagg aacttgtgaa atgatttttt cgatatcttc
     7981 caagaagccc atattcaaca tttcatctgc ttcatccaaa actaatgttt gaacggttcc
     8041 aagtttcaat gtgtggcggt taatgtggtc aagcatacgt cctggtgtac ccacaacgat
     8101 atgtggacga tctttcaagc cacggatctg acggccaata tctgctccgc cgtaaacggc
     8161 ttgtacacgt acttttttgt cgcgccctaa acgatataat tcttcttgtg tttggatagc
     8221 taattcacgt gttggtgcga taactaaacc ttgtaattcg tgacgatctg gatcgatttt
     8281 ttctaacatt ggtaatccaa atgcagctgt tttccctgta cctgtctgtg cttgtccaat
     8341 aacgtctttt ccagctaatg ctagtggaat cgtttctgct tggataggcg ttgcttcttc
     8401 aaaaccagcg cgttcgactg atttcaacaa ttctggtgat aattctagtt ctttaaattt
     8461 caaatgaatc ctcctatatg gttgttaact gaggatgtag cttttttgct accccgtcga
     8521 tttgttcggt caaagtgtat tgtttctttt tgacagtggc cacgcacgtt tttatccatt
     8581 cttagcccct gtggcaccac cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca
     8641 aacgggtcgc gttcagcttt atcattatta tgacatctgg cttcaacaag ttgtttctat
     8701 ggcaaaacca gtccaatttc tcttcatctt tcggcaatag gttattctaa catagttttt
     8761 cgttttctgc aagtttacag atttttattt catttgctga agttgttcta ccacttcaaa
     8821 taagcccatg ctgttgctgc cttttagcat aatactatct gttgcttcaa gattttgttt
     8881 gatagcatca atcaatttat tcttttcttt ttcaaaatag aacacaggta gttgggggta
     8941 tttttggttc aattcttctt ttagtgcatg catttgttcc ccataaagga aaatcatggc
     9001 gaatttctca ggatcgatat gttcactcat ttgcgcatgc atatttaaag actctttccc
     9061 taattccagc atatctgcta acacagcgat tctgcgtcct tttgttggca gatttttaaa
     9121 agtatctaat accagtccca tggctgttgg atttgcattg tagacatcgc tcaaaatagc
     9181 tgcaccattt cctgccgtta gccattcggt tctgttttga gtgatagaaa catgggcgag
     9241 cccttggcgt atctcaggta tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa
     9301 agcattcgtt acattataac ttccaggcag cgggatcgta aatgtttctc cttctactac
     9361 aaaatcggtt tgtgcttttc cttttgctgt cacttccgca tgaacatcgc catttccaat
     9421 accgaatgtt gtgactgttt gtactaatgt tgccgttaaa ggtgtcagca acggttcatc
     9481 tgcgggcaca acgagtaatc cgtctgatac aagtccgtca gtgatttcca ttttggcttt
     9541 agcgattcct tctcgcgacc caagattttc tatgtgtgct tcaccgatca ttgtgattgc
     9601 tgcagctttt ggttgagcca taagtgaaag ttccgtgatt tcattggcgt gatccatgcc
     9661 catttccaaa acgatctttt ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc
     9721 aatattattg ttgtaatttc cttgcgtctt atatgttttg tatttttcag acaagacaga
     9781 cgcggttaga tctttcgtgg tggttttacc attgcttcct gtaattgcaa cgacttctgc
     9841 gcctactttt tccaagtaat aggttgccaa ttgctgcatg gctttcaaag gatctgctac
     9901 aggaataact ggaaaatttt ttggagcttg ttcaacaggc aagctccaaa atgaagcaga
     9961 tgctccgttt gtgatcgctt gctggataaa ttcatgtcca tctcgcgtac cagcaagagg
    10021 aacgaataaa cttccttcgg ttatcttccg gctgtcaaat tcgacattgg taatctttgt
    10081 cagtgcagag cattcgctgc ccacggcttg tgcaatttct tgtattgtta gattcattga
    10141 gatctctcct aaaaaaggtt gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac
    10201 tctcacttcc aagtaaattt ctctattcgt atttagaagc tagaatgaac gcggttacgc
    10261 aatcactcca gcctcttctt tatttctctt tattcatttt tttcaaaaaa agattgacgc
    10321 tgatggtatc gattcattcc taactggatc agttcttcaa tcaaatcacc gtatttcaag
    10381 cccatatttt cccataagag tgggtacata ctgaactccg taaatcctgg catagagttt
    10441 aattcattca ggaataattc atttttattt gtcaaaaaga aatcgcaccg gctcaatccg
    10501 cttccaccta acatcgtgta agctaacttc gcgtactctt gcgctttttg ataaacttct
    10561 tctggcactt cggctggaat ctgcatttcg attttattat tgatatattt tgcttcataa
    10621 tcatagaatg ctacgtcttt tacgacttca ccaggcaaag tcgtccgaac gtcttcattt
    10681 cctaatacag caacttcgat ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa
    10741 tcatactgat aggctgttgc taaagcattt tgcagctctt ctcggttttc tgcttttgta
    10801 atgccgacac tagaacccat attcgccggt ttgacaaaca tcggataaag caaagaacct
    10861 tcacattgat caaatacttt tttaggattt tctttccatt gattcttaag tactggtaca
    10921 taaggaactt gcggcacacc agcagcttgt aaaatatact tggtcatgat tttatccatg
    10981 gcacatgcac tggtcaatac gcctgcgccg acataaggca tattcaatgt ctctaagaag
    11041 ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct
    11101 tcttctttga tttcgcccgg attgatcact tttcctgtaa agccttcctc tgtctgtcca
    11161 cttgggtccc atgaaagatg caagacatct ttgctggcag gtttttctgt tagtaatgga
    11221 cctttgaccc attgtccttc ttttgtaata aatacgagtt gaacttggta ataattataa
    11281 taaatggcat ttaaaactga aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt
    11341 ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg
    11401 atttactctc atattttagc acaaaacaaa aataaacgaa taacttattt ataaaaaaac
    11461 gagccagctt ctaaaagaac gagcgtcatt tattttttgt cagctagatt atttgtcccg
    11521 ttttttataa aaagttatga taaagaaaag gagggatgat gatggcgcaa acaaatccac
    11581 gcagccgctt ggcagaaatc attcgagtgt tcatcaatta caatgtagtg ccaaactttg
    11641 tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga agaactaggc cccaccttca
    11701 tcaagatagg acagatgctt tctgttcgag aagatttgct ttcttctgcc ttcactcaaa
    11761 cattcaaaac attacaagat agtgtaccta gcgacacatt ttcaacggtc aaaaagacga
    11821 tcgaaacaga actttctctt tcattatcag atatattcga tgatttttca aaatcgcctt
    11881 ttgcctctgc ttctatgggg caggctcatc gtgccaagct gaagaacggt gacgcggtag
    11941 tcgttaagat ccaacaccct aacatcgccg aagagatccg acttgatctt caactatttg
    12001 aacgagcgat ccctttgatc aaatacattc ctgaaactag cgtagttgat ctaaaagggg
    12061 tactccaaga agtaaaacgt tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa
    12121 atggcgaaca attctatcaa aagaacaatg gctggaaaga gatccgcagc cccaagattt
    12181 atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga aatgtctggt aaaaacttga
    12241 atcatttaat gaacgccgaa aacaaaactg aaacttttat cactggaatt caaaacaagc
    12301 agctgaaaca agaagttgca aagcttttag ttgagaactt tatgaaacag gtgtttgatg
    12361 atggtttttt ccatgctgat cctcatccag ggaatctgct attccatgta ctaacaaaag
    12421 aagaacagac acaagctagc cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct
    12481 ttgcttttcg agcctccact tctgcagaag accctgttgc gccatatacc atcaactaca
    12541 tcgattttgg tatgatgggg catctttctg caggacttag gcaaaaactc acacaagccg
    12601 ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc
    12661 agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga actgacaagt tttcttgaac
    12721 agtattatga ttctcctatt gatgaaatca acttgcaaga agtattcaca catgtcgtga
    12781 cgatttgtca tcaaaataat ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat
    12841 ttgggacatt agaaggggtg atccgcgtcc tcgatcctga agtttccctc atggaagtcg
    12901 cttcgccttt tgctcaacat tattttctca ctcatctgga tgtagaagat acattgaaac
    12961 aaagcggatt agacttattg gaagggatga aggctgcgcc taagatccct caacagctcc
    13021 accatctatt agaaatgtgg acttcgggac aaggaaaagt caatttggaa ttgaaaaagc
    13081 aagacaaatt attgagcagg atcgaaagta tgatcaatcg gcttgttttc ggtatgattt
    13141 tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc agcacctgta gaaaacgcag
    13201 aggtcgtttc cttattaggg atatttacgt atgctattgc cgcattcgtc attatttttt
    13261 tggcaatcga tgcattgatc caaatgtata agaagaggaa aaagtagaaa aaggttgtga
    13321 cagaaagaat cagcttcaag aaataagaag gggttttaca taaattgttt ctcaaatttt
    13381 tgtgaaaatt cggcttattt ttcgaagagg ctacttctga aacaccgaga ataaaaaatg
    13441 tgaaaaagct gttctgcacc cagtaataag aacaatcaaa aagggaacat ggaacacatt
    13501 tatcctgttt ccctgttccc tttttgatgt attcgagcat aaataaaata ccttcgttac
    13561 ctactatctt ctgtacttgg taaaccttag tcaattgcct tttttaacgc ttgacgaaaa
    13621 gacatatgat ctttctccgt aaagggtttt ggaccctttg tccttatgcc agcttttctt
    13681 aactttccac tatgataacg ttgttctaga agcgtgccga tattcgcttc gtcaaattct
    13741 tttccagagt gatggaacac tcgcacaccc ttcggcaata caagtgaagc taaaccataa
    13801 tcttgcgtga tcaaaaagtc attcttctta accataccga cgattttata atccgcacta
    13861 tcagctccgc ggtctacgta gacgatttgg acatgattag cgtattcttt ggtcgtataa
    13921 tggtccacac tggtcacgat aaccacgtca agaccatagg attctcctaa ttttatgaca
    13981 tcttctttta ctggagaacc atctccatca atgaatagcc tcattctttt tcgccttctt
    14041 ccataaatga taaacagtta ctaatccgct cagtaaaaga ccaacattga tccattttgc
    14101 caagataagc agttgtacac tttctatcgg cactgattca tggttcagcg tgatcaataa
    14161 tagatggatt ggctctccca tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa
    14221 ctggtggatc acactccctt tattttgagt agtatacttg acaacgatcc ggcgatccat
    14281 tggaatagct gtatcagttt gataatattc cttatttagc cagctgcctg ttactacgtg
    14341 ccagaaacta agcagaaaga cacctatcgc aaaaccattt ggaatcaaga ggaccaataa
    14401 agaagaactc agtccaagca attgcatagc caatggagac catgttaaac gtttcaaaat
    14461 ccatggtccg actatttttg ctgcaatcaa gcctgctgca taaggcaaat acataaaaat
    14521 cgaaagtttt tctaatccgt acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa
    14581 attccctagc attccattta gaaaagtaaa cagatttagc cagcttggca acttccattc
    14641 cttcttctgg tgtcctaaaa caaacacgaa caagatgaac agcagcaaga aaccataaat
    14701 agcaagatca aaaagccttt catcgaataa caatcgggca tttctcaaaa gaaacaataa
    14761 gccaaagaat atcaaaaata aaacaagctc tcgcgtagct accagatttc ttttgatgtc
    14821 tttgaaatcc agttcgtaac gcggataatg actaaccgta tggtaggcca tgacgtaaaa
    14881 aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg
    14941 atacaggaga aatagccaga tagctgcaaa taagtacttt ttcccatcca tattgataaa
    15001 accttgcgtc ttttcgtgaa aattgaccgt ggtattagca ggtacaagcc atgctgcact
    15061 cagccctagc aaaacacttg aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc
    15121 aagcaatgaa ccagcaccac caatcagcag agcgatcatc agcaacgtgt aactatctag
    15181 accaaatcgt agactgttga ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt
    15241 aaatggcaaa atcgtcagcc aattatgcga gtctgttagt cccaaaaata aaagataagg
    15301 aataaaaata accgtgttac ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa
    15361 tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca gtcttataac taatctgctc
    15421 ttcgatttta gcattaaagg ggaaataaac caattttaca gaatatattc ttgtaaaaaa
    15481 aaatacagaa agaagcataa gcttctttct gcatttttac ggtacttgat tactcaagca
    15541 gacaagccct tctccacaaa agtcttgccc gacacccctg catccggcag gtgataaaga
    15601 tagaaatcaa ttatcctgtg cctccaaaat caattaggag ctctgtaaac cagagtcctt
    15661 ctcgacccgt cgcataaatt tactttaaca cagttcctat gtttatgcga gagctttttt
    15721 cattttataa tcgaccattt gaaatccttg accgatatct gaaataagtt cttctgtaat
    15781 ctcaaatcct tcatgcttat aaacctcgat tgcttgatga ttcccctgat tcactcgcaa
    15841 aaagatctct ctttcctttg tttgcgcagc aagttcttca taccagcgaa aaactgaaga
    15901 agtcaatcct tgaccacgta ctttatttag caaatataat ttacttaaat aaatttttcc
    15961 atttatctct tcataagcag tgtaaccgac agtcttttct tcgcttttta ataaaaagta
    16021 ggacacacct tctgtcagtt ctttttgaat ctgttttttt gactgataag ttttcagcat
    16081 gtaatccact tgtttttgac caatgatcgg cacaaaaact tccggccata cttcttgtat
    16141 gactgccatc agctcatcca gttcattttc tgttactttt attttttcca tacacccacg
    16201 tcctttttta cattataaat gaccttgcgc ttgatggaat aaaaagagca tgatcatgct
    16261 atacacaccc accgaccacg gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga
    16321 taatttcgtc aaaccagcta ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa
    16381 ggtaacaact agtacttttt taatatcttt cgtttttctt gtcagcaatt catcaaattt
    16441 ctgatacttt tttggcgata aaataagctg gacaaaggct acgccatagt aacggacaag
    16501 caaaaatccg attgcttccc caatcactag gccaatacaa ctatataaaa gcccaaatcc
    16561 attgccaaac atcagcatgc ccgctactga ggagatccct cccggcaaaa taggaatgat
    16621 cacttggatc acttgcaaca gaacaaatat catcactgca tacttgccaa attgctggat
    16681 aaacgcttgc aaagacgtgc tcgatcgaaa aatcccatga tgataagcat aaagaatcaa
    16741 cacaaagaaa aaaaacagac caataatggg catgatctga agacattttt gtaaaactga
    16801 tcctttttta gacatctata ttccccacat cttttttcat ttattaaaac tgcctcct
//
LOCUS       DAZKAS010000051        16401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000051 DAZKAS010000000
VERSION     DAZKAS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.052"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACG8H7_001434"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACG8H7_001434"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFP8983534.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACG8H7_001435"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACG8H7_001435"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFP8983535.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACG8H7_001436"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACG8H7_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFP8983536.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACG8H7_001437"
     CDS             2857..3732
                     /locus_tag="ACG8H7_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP8983537.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG8H7_001438"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG8H7_001438"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP8983538.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACG8H7_001439"
     CDS             4629..5063
                     /locus_tag="ACG8H7_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP8983539.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACG8H7_001440"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACG8H7_001440"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP8983540.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACG8H7_001441"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACG8H7_001441"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP8983541.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACG8H7_001442"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACG8H7_001442"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP8983542.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACG8H7_001443"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACG8H7_001443"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFP8983543.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACG8H7_001444"
     CDS             13334..14260
                     /locus_tag="ACG8H7_001444"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFP8983544.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACG8H7_001445"
     CDS             14442..15335
                     /locus_tag="ACG8H7_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFP8983545.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACG8H7_001446"
     CDS             15469..15594
                     /locus_tag="ACG8H7_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFP8983546.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACG8H7_001447"
     CDS             complement(15728..16360)
                     /locus_tag="ACG8H7_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983547.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5830 a         2791 c         3495 g         4285 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat a
//
LOCUS       DAZKAS010000052        16369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000052 DAZKAS010000000
VERSION     DAZKAS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.053"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..1382)
                     /locus_tag="ACG8H7_001448"
     CDS             complement(72..1382)
                     /locus_tag="ACG8H7_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFP8983548.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1394..2812)
                     /locus_tag="ACG8H7_001449"
     CDS             complement(1394..2812)
                     /locus_tag="ACG8H7_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8983549.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3052..4620)
                     /locus_tag="ACG8H7_001450"
     CDS             complement(3052..4620)
                     /locus_tag="ACG8H7_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFP8983550.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4624..4905)
                     /locus_tag="ACG8H7_001451"
     CDS             complement(4624..4905)
                     /locus_tag="ACG8H7_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP8983551.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4902..5825)
                     /locus_tag="ACG8H7_001452"
     CDS             complement(4902..5825)
                     /locus_tag="ACG8H7_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFP8983552.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5822..6700)
                     /locus_tag="ACG8H7_001453"
     CDS             complement(5822..6700)
                     /locus_tag="ACG8H7_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFP8983553.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6687..8024)
                     /locus_tag="ACG8H7_001454"
     CDS             complement(6687..8024)
                     /locus_tag="ACG8H7_001454"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFP8983554.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8027..9130)
                     /locus_tag="ACG8H7_001455"
     CDS             complement(8027..9130)
                     /locus_tag="ACG8H7_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFP8983555.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9204..10712)
                     /locus_tag="ACG8H7_001456"
     CDS             complement(9204..10712)
                     /locus_tag="ACG8H7_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8983556.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10678..11370)
                     /locus_tag="ACG8H7_001457"
     CDS             complement(10678..11370)
                     /locus_tag="ACG8H7_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFP8983557.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11392..12366)
                     /locus_tag="ACG8H7_001458"
     CDS             complement(11392..12366)
                     /locus_tag="ACG8H7_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983558.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12666..13538
                     /locus_tag="ACG8H7_001459"
     CDS             12666..13538
                     /locus_tag="ACG8H7_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8983559.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14120..16318)
                     /locus_tag="ACG8H7_001460"
     CDS             complement(14120..16318)
                     /locus_tag="ACG8H7_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983560.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4815 a         3445 c         2659 g         5450 t
ORIGIN      
        1 acaaatcata tatcattaaa aaaaataaag gtcaagctaa aatattattt aagcttgacc
       61 ttattataat ttcaacgatt gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata
      121 gataaatcac agcttttgaa acataagttt tgtactgctt cgattcgccc gaattctttt
      181 gcagttattt ggcaatgaga aaagcagaca tcttctatag gcttttcagg aaatgctact
      241 aaatcaaaag ctcgggaagg aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg
      301 atgtgtatgt ctttgacttt cgtcatttgc tttgattcag gtatttcttc agccactgct
      361 tgccaacttt ctggcatttg agcaaggtca ttgctatttt ttctattgta ttcattatgc
      421 cagtccatta tccaagaaaa aggaaattgt acatcaatca tattcagatt ttctaccctg
      481 atattttcga tcactccccc tctttctttt gaagatttca ttcgaatacc acagtctgac
      541 tgaaaaaaat tgatatcgtg aatatatacg tcactaactc cagcactaac ttcgctacca
      601 atagttacac cgtatcctga acggatttta catctagcaa cctcgattct tgatgatttt
      661 cgattgactc ttgcaccatt gccatctctg ccagacttaa tagcaataca atcatcacca
      721 caggccaaat cacattcata gacacgcaca tttgttgaag aatcaatatc aatcccatct
      781 gtacttggtc catcattgtt gcggatgatt actttttcta ctaatacatc gttagaataa
      841 gtgatttgca agttccaaaa acctgatttc tgaaaaatca aatcacgaac aacaacatgt
      901 tctgcatgat acagtaaaca agcgcgtgga cgtttaatag cataatccgc aatccaacga
      961 agtccctttt gatcgtattc agctcttgtc ccgctttttt gatcttttcc ccaatacagc
     1021 tcccaccaat ggtctccttg accgtcgatt attccttttc cttctataag aatatctttc
     1081 gccgagagaa cattcaaaat cgctgctggc catttcattt ctacgccggc tactcgtgta
     1141 tcgatttctg gaaatgcttc gatatctgtt ctccccttga ggactgcacc ttcttcaaag
     1201 ataagatgac tcttatcttt taaaaaaagg gctccgacta aataaatgcc ttttggaata
     1261 aatacaggta agtgttcttt cgcaccttga tcgattgctt tttgcagtat tattgtatca
     1321 tcttgttgag tgtcgtttgt tttaaagttc aaagcattga taatcccttt ttttgtccgc
     1381 aatacgtttc tccctatctt tcattgattt ttttcaattc ttcaccaata aatacagttt
     1441 ttgaacgatt cagtgggtaa cctactagca aaatgattgc ggatacaaat aagcagaatg
     1501 ctggtaacaa gtttgccatc gtataaatcc tttcttgtac taaaactgat tggatggctc
     1561 cacctgttgt agacgattgg taaccaatga gtgccagcat aaatccgcct accgctcctg
     1621 cacatgcttg tcctactttt ctcgcaaaag aatttactcc atatactgtt ccatcttctc
     1681 ttaagcctgt cttatattga tgataatcaa tcacgtcagt aatgaatgcc cagaccatca
     1741 aattgaataa accagatcct aaagtagcga taaataaaag aatcagatat acccatgcat
     1801 ttgttatatg aaggaaatac atcagcaagt acatacatga agaagcaagt aacgcaaata
     1861 cgcttgcttc ctttttacca aataattttg tcagctttgt agcaaatggt gctaaaacta
     1921 ctactgttcc gtaagtaaat aaaagagcaa cagacattgc ttgtttatta tggaagtaat
     1981 cattgaataa ataagtcata ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt
     2041 caacaatgac taaaataatc agtgctcgat tagctaacaa accttttacc aactgagaag
     2101 cagatacttt ttcgtttttg acaactctta ctctttcagt agtcaattta caagttaaca
     2161 tatatgcgac aaatgctatt gcagcacatc cgattgaaat ccaaaagaaa tgattccctg
     2221 aaacgacttg ttgtccactc ttgttatggg tatacattaa aatcggaatg aagaatcctg
     2281 tagttgatcc gccaatcgca gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat
     2341 taggatttga gctaattgct gctgccattg ctccatacgg gatattgatt gttgagagaa
     2401 aaatcccata aacaatgtat gaaacaaaaa tataagcaat cttcaccgtc attggaagat
     2461 gttggacgaa tggcaaaaac aaaatgacag tcactaagca aaatggatac ttcatttttc
     2521 tgatccaagg aataaatcgg ccagtttctg ttaactctga gacatcacat aatcttccaa
     2581 ccatcacatc agcaaacgca tccaaaattc tcgaaatcaa gaataacgta cctacgattg
     2641 cccctgaaac gccaagaaca ttcgtataat aaatcatcaa gaaactattg actaaaccta
     2701 aaataaaaca gttacctaga tcaccgaaca tgtagcctat cttatcggaa atcccaaatt
     2761 cctttttttc tttttttact gtatttgctg taacagcatt ttgtgaaagc atttgaattc
     2821 ctcctacctc ttctagtaaa cgttttcaaa attataactc tttttttctt gtttttatat
     2881 tcacaatgtt ggctttttat tgcattatct tgacagataa aaaacaaaaa ataatctaaa
     2941 aacattgatg cataagattg ataacttttt ttacataaca atatatacaa acaaaaaaat
     3001 tcactaacta gtgaaaataa gttagtgaac ctcatttatt aaagaattaa attacagatg
     3061 atcttttcca tcatacttga aaaactcaaa atcagcataa cttcctcttt ttttatttag
     3121 atcatgcact gctatcccga taaaatttcc agtaaaccct cctgagagta tacacatgtt
     3181 tttttgcgga cctactttct tccattcttc agttgatacg tctttcaaat gaaactgtgc
     3241 tgtctctttc tttacagtga ctttcaaaaa agtcttcttt tctgtgagtg gaatcgtcca
     3301 atcttctatt tgtgctactc cgttttctga ttctatcata cgtaggcatt ttcccttgtc
     3361 ctcattccaa gtaacataac aataaatata attggattca tttaagtaca gtaccagtcc
     3421 agccatctga ttaaaattat taggcatgta ttcaaatgct gtctccgcat caaaagtaaa
     3481 atccgtttgc cgaatagcta acaaatgatg ttcgaaggta gattgaagag attcaccgga
     3541 aatcagacgt aaaaaacctg gtctacattt tgtatcacac caatcagcat ttggcaaaat
     3601 gcgtctggca ttccagttct tctttagctc tccttcaaat gtatccataa actgatgatc
     3661 tttttcttgt tgtacctctt catttgtctc aatcattatt tcttccatcg gagttgtctg
     3721 gctatcttta agtcttaacc aatcgtcgga cgtccaatac acttcttgaa tagcggtttc
     3781 tcttcccaaa atagccgcac cgtttaatgg tctggtacaa agatatgtca tgtaccatct
     3841 tcctgacggg gtttgtacta agctcccatg tcctgtacat tgcaatgtag aagcaggttt
     3901 gtcactagct gtcagcatag gaaaaccagg atcaagctcg taaggaccag taatattctt
     3961 tgctcggcaa actgtaactg aatgtcctct tcctgtaccg ccttctgccg tcatcaaata
     4021 ataatacccg ttacgaaaat acaaatgtgg tgcctccgtt ttcgctaatt ctgtacctgc
     4081 aaatatttta tagatgggac catcaagctt cttcttctct gcatcatatt cttgtaatac
     4141 gataccgctg gatttattcg gtgtgtccag acgataatcc catagttcat tgacaagcca
     4201 tttttttccc gtttcaagat catggaacaa agaaggatca aagccgttac tatttaaata
     4261 aattggttcg gaccaaggac ctgtgatctc agatgcagtg atcaaataat tgtggacatc
     4321 tttaaacgga cggcctgcac ttttgacatc tgtgtaaaca agataaaaca atccatcagc
     4381 ataactgatc tgtggcgccc atatacttcc atttgtagga tttccttgca aagatacttg
     4441 agtggtcaaa atatccgttg tatgtgacca gttgactaaa tttttcgact catatactcg
     4501 gattcctgga agccattcaa aagaggagac aataatgtaa taccgctctt tcactcttaa
     4561 tatattagga tctggattga atcctcttaa aataggattt ttcactttga tagtctgcat
     4621 gattcaggct ccttttctca ttcttactcg atataaactg aatgccccag ctgctaaaaa
     4681 agcaagtgaa ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa
     4741 tgtattttct aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa
     4801 ctctgacgca gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag
     4861 actgccaccg accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc
     4921 ttttctaaat gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca
     4981 gagaatttga cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga
     5041 acatgcccac ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt
     5101 cctaaataga cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac
     5161 cctgtttttt gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct
     5221 aagctatgga tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc
     5281 gttccttcaa tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc
     5341 ggtgtacaaa aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta
     5401 tcctgataac cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa
     5461 gcaacagcct gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct
     5521 aatacgacat tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta
     5581 ccgtcaagat actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc
     5641 acaagtttaa aattggataa tctgctttct atatattcat catatcttcc ttcttttact
     5701 atcaagacct ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata
     5761 gtttcaaaat ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc
     5821 ctcataaatg ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat
     5881 gaccgcctgt taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt
     5941 tgtccagccc ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc
     6001 caactaagct aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag
     6061 ctgtcaatag atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca
     6121 aaagtgtttc gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca
     6181 atgctgtcac ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt
     6241 gagtatctgt gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac
     6301 gcattcccca taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa
     6361 tacctccgcg ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg
     6421 tcagtacttc tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt
     6481 tcccgactga aaaatctccg ccgtgagaat gcaggtaaag gaccgtcgga aacggtcctt
     6541 ctttttctgg ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat
     6601 agtattcaag cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat
     6661 gtctgctaga tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca
     6721 accagttcat ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta
     6781 gtattttcta atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa
     6841 accttacccg caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc
     6901 atagcaggaa caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg
     6961 atttcttgta tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc
     7021 cttgccgtga tattcgaaaa atggatcctt ctgaatgctg atgtccgttc atctatcgga
     7081 taagcctttt tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga
     7141 ttcagtataa atggacacat cacattatcc atgactatat tggatactcg aatatcttca
     7201 acgatcccgc ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag
     7261 atacaatttg aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca
     7321 ccgccatgtc catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt
     7381 tcgtacgtat cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg
     7441 caattactaa tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa
     7501 tccgctgggt tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg
     7561 atcgtccaac ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta
     7621 tggaaactca tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta
     7681 tgccaccatt tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct
     7741 acattttgag catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa
     7801 tgaacaacag gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa
     7861 tgaagttcga cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca
     7921 actacggtcc cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta
     7981 ttcaattcat caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca
     8041 cttcaatcaa tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt
     8101 cactgatgta gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt
     8161 gacagttttt attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta
     8221 atccttgatg tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac
     8281 gtattccttt agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc
     8341 ctttccgttt tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg
     8401 gaagtaatgc agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt
     8461 catgaagtaa ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc
     8521 aaaaatgatg ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc
     8581 atgcatgaat cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact
     8641 gtctcaaaac atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa
     8701 aaggagaacc catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc
     8761 aaaaagctcc ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg
     8821 cttttttata tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat
     8881 ttacatggtc gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca
     8941 cgaaatagtc gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc
     9001 aaagtaattg gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc
     9061 acttttcttc atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc
     9121 agttttccat tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga
     9181 agtatttgtc ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct
     9241 tgtgtgattc gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct
     9301 tcgtcaagct gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct
     9361 gatcctactt cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat
     9421 tcttcatttt ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa
     9481 gagaacgtca cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga
     9541 tccatatcga taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata
     9601 ccgccagtca tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat
     9661 ttcttcaatt gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata
     9721 ccatttcctt ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca
     9781 ggcactaatt ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc
     9841 accattccag tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt
     9901 tgggtaactg caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc
     9961 aaagctttca tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca
    10021 gcccagccat ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata
    10081 tcactaggcg cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg
    10141 ttttctgtaa attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt
    10201 cctagattat ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt
    10261 atcggtacgc cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt
    10321 tgacttaaat ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt
    10381 aacgcattgc ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct
    10441 agttcatcag aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag
    10501 ttgaatgtca gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc
    10561 ggtgaagcat gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct
    10621 gaagaactgt tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta
    10681 agacctaatt ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat
    10741 atttttgttt tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat
    10801 agaatttgct gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac
    10861 acctggcatt gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa
    10921 tgagtaatga agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc
    10981 tttccaatca taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa
    11041 cgcagcaata tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag
    11101 taaaacaatc ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc
    11161 tttaaaatat gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt
    11221 tctaatcact gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc
    11281 gatgactaaa ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct
    11341 tccgtttatt ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac
    11401 gccgtcttct cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac
    11461 gaccccttta aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc
    11521 tgtataaata taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa
    11581 gtacatatgc tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat
    11641 gattactgga cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc
    11701 atccatttct gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat
    11761 gatcgtagac cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca
    11821 ttcaggggat gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa
    11881 tacccctccg tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat
    11941 gaagtgaggg atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc
    12001 atttaatagt aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa
    12061 caagatcaac gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa
    12121 atgtttgaaa ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa
    12181 agcaataacg agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg
    12241 cagaatcatc acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct
    12301 tctgagcttt tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact
    12361 ttccataagc ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa
    12421 ctatctgaat tacgtatata atcattttta tcgtaagtaa aaacgcttac atagcaatga
    12481 tttttgtgct atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa
    12541 ccttctattt ttttctatct gacacttagc ataggaaatg attatatacg taatctttca
    12601 ccgatgataa attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta
    12661 tctatatgaa aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt
    12721 ttctaatttt gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt
    12781 ttgcttctac acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt
    12841 tagacgcgta tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga
    12901 ttggtgttac aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg
    12961 gtctttccag aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta
    13021 tgttgttcag cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca
    13081 attcttactg ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga
    13141 gaaacttttt ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca
    13201 cagattttgg tgtatttttc aaaattatgc tgccattagc tttaccgtct attgctacta
    13261 tctcgctttt ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca
    13321 acgattcatc taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg
    13381 ggatgcaagc agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg
    13441 ctgttatcgt tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt
    13501 ttgtcaaagg agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg
    13561 tagttgtgtc agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct
    13621 taaatttttg tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat
    13681 cagaggttgt gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc
    13741 gacgagtaac ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac
    13801 aaaattatgc cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa
    13861 ctgtgacaag acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc
    13921 ataaatacaa atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa
    13981 aaacagcctc ttgattaaac ggcgtttatc ttgttgattc tgcggcaata aaatcagcca
    14041 gcagcaaaac atgaagtgct gttttttgct gtcctctttt attgcttgaa tcagatcaca
    14101 agaaaaacag cctcttgatt cacaaatatt tttttcgata ttctcccggc gtaatcgatt
    14161 ccttcttttt aaagaaacga ataaaatttt gcggatttcg atattgcaat ctttctgcaa
    14221 tctcttttac ggagaggtta gtctctttca accatttttt tgctacctct aaacgatgat
    14281 tttgaacaaa atcaccaaat ttttctcctg tttccttttt aaatacatta cttaaatagt
    14341 tagggttata atgcaaccta tcagcgatca catctaaaga cagatcacga tcatattctg
    14401 tgtagacgat atgcataata ttctccgaca aatttttaaa ttcatgatca gtgattgctt
    14461 gagagcattc tgtgattggc agaactaatt gttctaaaac aagtttttct agttctttgg
    14521 gatgataatt gttcagtgct tttacataaa tttgtttcat gttatcaaag ttttttgaat
    14581 ctactcccaa taactgtcct aattgaacga gttcattgat caagcggacc gaaactactt
    14641 cccgactcaa aggattttta ttttgtgcaa acaatgcatc aatcaattca tgtactaaat
    14701 tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa tcgattttgt tcttctatcg
    14761 gatatctaat aagtgttttt tcatgagcag caggaacgat ttcctgataa aaaataattg
    14821 aacttggacc agtatttaca cgataataca gggcttcttt tgcccgatca accgattgct
    14881 tgctttcatt cagcgtttcg aaccggttac tgatgcctat actgatagtt aaattcaagt
    14941 attcttttac acgtttttga atgagtcggc aatactccat gacttttttg cttgcttctg
    15001 catcatcttt atccattcga taaatagtgg cttgcatttc ttcattcaaa acgatcggca
    15061 gcattcgatt ttcctttggc acaatttccg tgatcatgtt gttgatcgct aaaagtagca
    15121 aatcacgctc cccaccgtga ctatcttcta acagatcgat ttgaaccaaa gctgtataat
    15181 agcagtcatt ttgagaacta taaccaaatt gctgcaaccg ctgatctagt tctcgccttt
    15241 ctactcgatt tctaaataaa ctaagtacaa atagcgtttc cagctgtggc ttttgggtaa
    15301 ctaagtgtgc acttaacgct tctttttctc ctatgatccg actgactgat tgagcgacca
    15361 aatctagttc tttcccactg aatccttttg atttaagatt cagacgttcc tgtatttgag
    15421 aaatcggacg ggcaaatctg tccgaaaata agtaggaaaa aagaccaatg agtcctacca
    15481 gtgttaaaga cactgcaact agcccgattc tcagattgcg gatcattgcg ccaattgcac
    15541 tagagtcaac ttctaatctg taaatccaat tattataatc tgatttcagt actatatacg
    15601 ttttatcttt ttctcttatc actttcagct gattgtaatc tagatccacg tccgttaata
    15661 cagttggttg ctctttattt tttactgtct gagtagtaaa aaggttgcca acttcgtttt
    15721 caatagaaag tatgtcttct ccactttcac caacaacttg ttctattgta tgtttctcta
    15781 tcgtagcaat tcccaatgca tatttttctt tttcaaacat tggcaaagaa atcaccattt
    15841 cgattccttt gtttttcttt tgccaaaata aattattttt gttatttatg tacttctgtt
    15901 gatacttttc aacttcctct tcggtcagtt gactcaacga tccattgatt actccccatt
    15961 taccagccaa actgactaaa tcataattat tatcatccat aacaatcgtt tctataaaat
    16021 ttagttcttt cctgatttca gaataagtgg agtaatctgt atatgtcagc ggatgcttga
    16081 atatgtccga gaaatttttg gtcgaaccat acgtagaaaa cgaatattca atcgttctaa
    16141 ttttccattc aacgttagtt ttaacttgcc tgagcaaatt ctttttgtct gttaaataac
    16201 tcgattcgat cgattcatag gtattctggt aaatgaataa actaaatcct atgacaagta
    16261 cggctcctaa taaaaacatc ggtaaaaata ttttataaaa tactgtactt ttcttcattt
    16321 tcttcctctc ctccttttaa aataaaaggc tttcattttt ctgtaaaat
//
LOCUS       DAZKAS010000053        16096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000053 DAZKAS010000000
VERSION     DAZKAS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.054"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACG8H7_001461"
     CDS             138..1475
                     /locus_tag="ACG8H7_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFP8983561.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACG8H7_001462"
     CDS             1523..3652
                     /locus_tag="ACG8H7_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFP8983562.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACG8H7_001463"
     CDS             3627..4316
                     /locus_tag="ACG8H7_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983563.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACG8H7_001464"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACG8H7_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACG8H7_001465"
     CDS             complement(5546..5740)
                     /locus_tag="ACG8H7_001465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983564.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACG8H7_001466"
     CDS             complement(5790..6230)
                     /locus_tag="ACG8H7_001466"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFP8983565.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACG8H7_001467"
     CDS             complement(6234..7079)
                     /locus_tag="ACG8H7_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFP8983566.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACG8H7_001468"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACG8H7_001468"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFP8983567.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACG8H7_001469"
     CDS             complement(8703..9650)
                     /locus_tag="ACG8H7_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8983568.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACG8H7_001470"
     CDS             complement(9873..10223)
                     /locus_tag="ACG8H7_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFP8983569.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACG8H7_001471"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACG8H7_001471"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFP8983570.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACG8H7_001472"
     CDS             11646..11966
                     /locus_tag="ACG8H7_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP8983571.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACG8H7_001473"
     CDS             11988..12452
                     /locus_tag="ACG8H7_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP8983572.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG8H7_001474"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG8H7_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFP8983573.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="ACG8H7_001475"
     CDS             complement(13301..14590)
                     /locus_tag="ACG8H7_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFP8983574.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG8H7_001476"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="ACG8H7_001476"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP8983575.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15825
                     /locus_tag="ACG8H7_001477"
     CDS             15700..15825
                     /locus_tag="ACG8H7_001477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983576.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            15822..>16096
                     /locus_tag="ACG8H7_001478"
     CDS             15822..>16096
                     /locus_tag="ACG8H7_001478"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFP8983577.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQL"
BASE COUNT         5081 a         2952 c         3282 g         4781 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaact
//
LOCUS       DAZKAS010000054        15992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000054 DAZKAS010000000
VERSION     DAZKAS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.055"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8H7_001479"
     CDS             226..894
                     /locus_tag="ACG8H7_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP8983578.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACG8H7_001480"
     CDS             complement(1094..1480)
                     /locus_tag="ACG8H7_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983579.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACG8H7_001481"
     CDS             complement(1452..2834)
                     /locus_tag="ACG8H7_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP8983580.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACG8H7_001482"
     CDS             complement(2990..4177)
                     /locus_tag="ACG8H7_001482"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP8983581.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACG8H7_001483"
     CDS             complement(4184..5215)
                     /locus_tag="ACG8H7_001483"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFP8983582.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACG8H7_001484"
     CDS             complement(5231..6685)
                     /locus_tag="ACG8H7_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFP8983583.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACG8H7_001485"
     CDS             6860..7765
                     /locus_tag="ACG8H7_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFP8983584.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACG8H7_001486"
     CDS             7856..8533
                     /locus_tag="ACG8H7_001486"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP8983585.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACG8H7_001487"
     CDS             8530..8700
                     /locus_tag="ACG8H7_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983586.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACG8H7_001488"
     CDS             8814..9710
                     /locus_tag="ACG8H7_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP8983587.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACG8H7_001489"
     CDS             complement(9765..11135)
                     /locus_tag="ACG8H7_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8983588.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACG8H7_001490"
     CDS             11373..12119
                     /locus_tag="ACG8H7_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP8983589.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACG8H7_001491"
     CDS             12116..12955
                     /locus_tag="ACG8H7_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP8983590.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACG8H7_001492"
     CDS             12956..13906
                     /locus_tag="ACG8H7_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP8983591.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACG8H7_001493"
     CDS             14047..14799
                     /locus_tag="ACG8H7_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983592.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACG8H7_001494"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACG8H7_001494"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP8983593.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5192 a         3002 c         3220 g         4578 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aa
//
LOCUS       DAZKAS010000055        15952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000055 DAZKAS010000000
VERSION     DAZKAS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.056"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..809)
                     /locus_tag="ACG8H7_001495"
     CDS             complement(387..809)
                     /locus_tag="ACG8H7_001495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP8983594.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="ACG8H7_001496"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="ACG8H7_001496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP8983595.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="ACG8H7_001497"
     CDS             complement(2252..3154)
                     /locus_tag="ACG8H7_001497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP8983596.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /gene="atpA"
                     /locus_tag="ACG8H7_001498"
     CDS             complement(3168..4724)
                     /gene="atpA"
                     /locus_tag="ACG8H7_001498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP8983597.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="ACG8H7_001499"
     CDS             complement(4747..5289)
                     /locus_tag="ACG8H7_001499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP8983598.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="ACG8H7_001500"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="ACG8H7_001500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP8983599.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="ACG8H7_001501"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="ACG8H7_001501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFP8983600.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="ACG8H7_001502"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="ACG8H7_001502"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP8983601.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..7644)
                     /gene="smpB"
                     /locus_tag="ACG8H7_001503"
     CDS             complement(7180..7644)
                     /gene="smpB"
                     /locus_tag="ACG8H7_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP8983602.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7666..10023)
                     /gene="rnr"
                     /locus_tag="ACG8H7_001504"
     CDS             complement(7666..10023)
                     /gene="rnr"
                     /locus_tag="ACG8H7_001504"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP8983603.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10026..10781)
                     /locus_tag="ACG8H7_001505"
     CDS             complement(10026..10781)
                     /locus_tag="ACG8H7_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8983604.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="ACG8H7_001506"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="ACG8H7_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP8983605.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="ACG8H7_001507"
     CDS             complement(11238..11516)
                     /locus_tag="ACG8H7_001507"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8983606.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="ACG8H7_001508"
     CDS             complement(11629..12597)
                     /locus_tag="ACG8H7_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8983607.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="ACG8H7_001509"
     CDS             12880..13854
                     /locus_tag="ACG8H7_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFP8983608.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="ACG8H7_001510"
     CDS             13859..14434
                     /locus_tag="ACG8H7_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFP8983609.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="ACG8H7_001511"
     CDS             complement(14501..15640)
                     /locus_tag="ACG8H7_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFP8983610.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5296 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat tt
//
LOCUS       DAZKAS010000056        15881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000056 DAZKAS010000000
VERSION     DAZKAS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.057"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..2799
                     /locus_tag="ACG8H7_001512"
     CDS             328..2799
                     /locus_tag="ACG8H7_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP8983611.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2902..4638)
                     /locus_tag="ACG8H7_001513"
     CDS             complement(2902..4638)
                     /locus_tag="ACG8H7_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8983612.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4635..5339)
                     /locus_tag="ACG8H7_001514"
     CDS             complement(4635..5339)
                     /locus_tag="ACG8H7_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8983613.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5470..5973
                     /locus_tag="ACG8H7_001515"
     CDS             5470..5973
                     /locus_tag="ACG8H7_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFP8983614.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5933..6271
                     /locus_tag="ACG8H7_001516"
     CDS             5933..6271
                     /locus_tag="ACG8H7_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFP8983615.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6292..7710
                     /gene="ffh"
                     /locus_tag="ACG8H7_001517"
     CDS             6292..7710
                     /gene="ffh"
                     /locus_tag="ACG8H7_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP8983616.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7822..8400
                     /locus_tag="ACG8H7_001518"
     CDS             7822..8400
                     /locus_tag="ACG8H7_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP8983617.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8404..8925
                     /locus_tag="ACG8H7_001519"
     CDS             8404..8925
                     /locus_tag="ACG8H7_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFP8983618.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9004..9558)
                     /locus_tag="ACG8H7_001520"
     CDS             complement(9004..9558)
                     /locus_tag="ACG8H7_001520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP8983619.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9811..10086
                     /gene="rpsP"
                     /locus_tag="ACG8H7_001521"
     CDS             9811..10086
                     /gene="rpsP"
                     /locus_tag="ACG8H7_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP8983620.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10098..10349
                     /locus_tag="ACG8H7_001522"
     CDS             10098..10349
                     /locus_tag="ACG8H7_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFP8983621.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10474..11265
                     /locus_tag="ACG8H7_001523"
     CDS             10474..11265
                     /locus_tag="ACG8H7_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983622.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11435..11956
                     /gene="rimM"
                     /locus_tag="ACG8H7_001524"
     CDS             11435..11956
                     /gene="rimM"
                     /locus_tag="ACG8H7_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP8983623.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11946..12707
                     /gene="trmD"
                     /locus_tag="ACG8H7_001525"
     CDS             11946..12707
                     /gene="trmD"
                     /locus_tag="ACG8H7_001525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP8983624.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12843..13190
                     /gene="rplS"
                     /locus_tag="ACG8H7_001526"
     CDS             12843..13190
                     /gene="rplS"
                     /locus_tag="ACG8H7_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP8983625.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13322..14458)
                     /locus_tag="ACG8H7_001527"
     CDS             complement(13322..14458)
                     /locus_tag="ACG8H7_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8983626.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14574..15488)
                     /locus_tag="ACG8H7_001528"
     CDS             complement(14574..15488)
                     /locus_tag="ACG8H7_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP8983627.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15579..>15881)
                     /locus_tag="ACG8H7_001529"
     CDS             complement(15579..>15881)
                     /locus_tag="ACG8H7_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP8983628.1"
                     /translation="GQFQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSY
                     YVSTRTSRSKSESEANCFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
BASE COUNT         5138 a         2854 c         3152 g         4737 t
ORIGIN      
        1 caaaaaataa atgtttatct gttaatccga gtaattttct agtatgatta tccattgaga
       61 gactcctttg gtaggatgac gttttggtcg actaaatttt accaaaagtg agtctctttt
      121 tgagttgaaa cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc accaacacaa
      181 aatattatag aacccttttt ttttgctata tgacaggaat gaaaggtgtt tgcaagctta
      241 atgtgaggaa cactttccat atctgttcta tactggatag gagaagtttg gaaatatcca
      301 gaaaacgaaa tcaatcgagg tagccacatg aagaataaat ggataaatcg aagtaaaaaa
      361 aagaaagtaa cacaaagatc atcagataaa gtcatccgtc tttttccatc gtataaagaa
      421 ggattaacgg cagatcaagt caaggaacga atagaaaaag gtgcagcaaa taattcagtc
      481 gatcccacat tcaaaacaaa tcagcaaatc gtattggaaa acatatttac gtatttcaat
      541 ttgatctttc ttatcttagc cattttgctt tgtttagtag aatcctataa gaacttgact
      601 tttttaccag ttatcatagc caatacagga atcgccattt ttcaggaaat tcgatccaaa
      661 aagatcttgg acgaactaaa tgttttaaat acagcaaaag tcaatgtcat acgagacggg
      721 atagaacaaa caatagatat agaagacctt gtaatagatg acattgttat tttagagact
      781 ggatatcaaa ttccagcaga tgcagaggtc gtggaaggga aactgcaagt taatgaggct
      841 ttattgacag gcgaagcaga tgaaataacc aaagaaatag gtgatccatt aatgtccggc
      901 agtttcattg tttccggaaa agcttacgcc cgattagaca aagttggagc agattcgtat
      961 atttcccagc tgacagtcaa agccaagaca atgggagaag gagaacagtc ggagatgatt
     1021 gcttctctta atcaattgat caaatggatt gggatcatca ttattcctat cggtatcctg
     1081 cttttttctc aaagttactt ttttaatgga aacacgatca aagaaagtgt cgttgcaatg
     1141 gaagcagcat tgatcggtat gatacctgaa ggactgtact tgctgacgac gattgcatta
     1201 gcattaagtg cgactaaact agcaaagcaa agagttcttt tacacaatat gaaaagcatt
     1261 gagacattgg caagggtaaa tgtcctttgt gtagataaaa caggaacgat cacagaaaat
     1321 aaaatgtctg ttcaaaaaat gatcgtctct aaaaaacaag aaataatcag tcagtcagat
     1381 caattggaag catgcattgc tgactatgca aaagcgatgg caggagacaa tgcaacaatg
     1441 gaagctgtta aagcatattt tacaaaaacc actgatcaat cctatcacca tgttattcct
     1501 ttttcttctg tccaaaaatt cagcagtgta gcgttggcag acaaggtgta tgttttagga
     1561 gcgcccgaaa tggttttgcg cgatcaattg ccggaatatg cggaagagtt tgtttctttc
     1621 gctgaaaaag gctatcgcgt gttggtcttt ggtgtgtacg atgggattct tgaagaacca
     1681 gtattgacag aagcagtcac accattaggc tatattctga ttgctaatcc tatcagaaaa
     1741 gaagcaagag ccacatttga ttacttcaaa aaacagcaag tagccatcaa agtgatttct
     1801 ggagataatc cgctcaccgt atccaatgta gctgtccaag cagggatttc acatgctgat
     1861 cagtacgttg atgcatctaa attatcagaa gaagaatatg aagcagctgt cgaaaaatat
     1921 acagtttttg gacgcgtcaa gccagaacaa aagaaaatct tcgttcaatt gataaagcgt
     1981 aacaatacag tagctatgac aggtgatggc gtgaatgata ttctagccat gaaagaagca
     2041 gactgcagta tagcgatggc ttccggaaat gaagccgcca tgcaggcctc acaagtggtc
     2101 ttacttgatt ctgacttttc acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg
     2161 aacaatattg agcgatcagc cagcttgttt ttagtgaaga atattttctc ctttttgctc
     2221 tcactgtttt cagtgatctt tgctctgacc tatcctttag aaccatcaca aatcacattg
     2281 attagtttgt ttactatcgg acttccatct ttcttgcttg ctctagaaga aaacaaaaag
     2341 cgaatcagag ggaaattcat catgaatgtg atggaaaaag ctgtgccagg tggactaaca
     2401 gatatgatcg tagtcggtgc gttggtgatc tgcggggtga ctttgaatct aaataaaaca
     2461 gatgtttcta cagcatctac catgcttttg attgcagtag ggttcctcgt tctctataaa
     2521 atctgttcac cgctgaataa attcagaagt cagatcattc ttttctgtgc tagcggtaac
     2581 tttttttcgg tcatcttttt gcataagctg ttttcaatca ccggtatctc agctgtatca
     2641 cttttgctgt tgagcattct atttttctcg gcagattcga tttttaggca tttgaccaca
     2701 ttggtagaat atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta
     2761 gctaaaaaat ttctatcgat atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga
     2821 gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa
     2881 cgcacgagca gttgctcttt tttacatttt cggaatggta acggtgaaag tcgaacctgt
     2941 tccaagatga ctgtctacag taatcgtacc attaaggagt tgcacgtagt cttttacaat
     3001 cgctaaccct aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa
     3061 acgctcaaag atccgttcct gatcctcaat gtcgatacct ataccaaagt cttggacaga
     3121 aaacgataga tcatctgttg cctgatagcg gataataatc tcgctgtcgc tttttgaata
     3181 ttggacagca ttttcaatta gatttttgat aattggataa aggatatctg ttttagtagt
     3241 aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg
     3301 ataattttga acgatttgct ggaaataagg ttctgtataa attgtctgga tctcgtaaga
     3361 attctcgcca tcttttgaca gctggatgat ttcccgaatc agtttatcta accggatcgc
     3421 atctttttgc atgatctcaa gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc
     3481 cagtaaagtc tcggtaaaac caatcagtga agtcacagga gtttttaatt cgtgagagac
     3541 gttgccaacg aaatcctttt gtagcttttc taaacgtcgc actttcgtta agtcatacgc
     3601 cacccctaga atctgtccgg taccttctga attagtaaag tagcgcaaac tcaagtctaa
     3661 tgtctgccga ccttctgtaa gggtgatctc ctgatggatc agcggatgct caggagtcac
     3721 ttgatgaatc agctgaatga gttttggctc ttgaatgact tctgtgtacc gctgttcagg
     3781 ttgatagctt accacgttta aatgtagctg catctttgga ttaagcagta gtaaacgaga
     3841 atcagcatca tcaatcagga atacgccaat catcagttcg ttcagtaacg tatataattg
     3901 ctcttctgtg gtagtacacg cccgatacat cttactcatt tgttcgctca acgcattaat
     3961 cgtctgatac aattcttccc attgatcggg tgtttgcatg attacttccg cctgattagg
     4021 attttgcacc atttttttta gtacgggcag cacggttttc aatggctggt ttttttgata
     4081 gatcagataa tagatcattc ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac
     4141 ccaacggcga aaactctcag ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa
     4201 aatggcttct aactggccac tttttttgac tggtaatgcg acatataaaa gatcctcatt
     4261 caaagtagta cttttgcgca aagcgctacc taggttccca ccattcaata cggctttgat
     4321 ttcgggacgg gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa ataagatctt
     4381 tccagtagca tccattaaag taatccgttc gttcgactga tggacgaatt cttccaaagc
     4441 agttttgttt tgcgctgctt ggaggttttc gacatctaat tgccggatca gcagttgtgc
     4501 tttttttgtc aggtagcttt cttgctgttc cacgatctgc tgttgaaaat aatgggaaat
     4561 caattgccag ccaccaaaaa acaaactaac taaaagaata agaaagaaga gacgttcata
     4621 aatttttttc catttcatcg tttgggttcc tgaaacttat agccaaatcc tctaatagtc
     4681 accaagtact ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt
     4741 acatctacga ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg
     4801 tctcggtcga tcacacggtc ttttcgtttc atgaaataaa caagtaattc aaattctttc
     4861 ggtgttagtt caatcagatg gtcgtctacg gtcacttgat aattggtcaa atcagcagtg
     4921 atttgtccaa tcgatagata ttcaggctca gcttcatctt ttgattgctt acgaggttca
     4981 attcgtctga atattgcttt catacgggct aatacttcgc gaggactaaa tggtttggtc
     5041 aaatagtcgt cagcacctat ttctaggcca atgatccgat ccacttggtc atctttagcc
     5101 gtcagcatca aaatcggtgt ttcatttttt tcttgacgca gccgctgagt gatctccatg
     5161 ccatccatcg ttggaagcat cacatctaaa atgatgaaat caaatgattg ttctaatgct
     5221 aattctaatc cttcgcctcc atctgtggca ctggttacct tgtagccttc tttttccaaa
     5281 ttgaacgtca agagcgttac aatagaaggt tcatcatcaa caactagtat ttttttcatt
     5341 caaatctcct ttaaataggt ttcattctgt ttatatttta gcatagaatc agtacaaagg
     5401 gctattttac aaaagaaata aatggtataa taggaaggca ctttgctgaa tagaaaggaa
     5461 gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga
     5521 cagaaaaaag aagtaccaga attgatggaa ctagttgaaa ctggagaagc tgtcatggtc
     5581 tgtccagaag tgatcggcgg attaccaatt cctagagatc ccgctgaaat cattggtgga
     5641 aatggattcg atgtgtggaa agatcaagca aaagtatgga cgacaagcgg cgaagatgta
     5701 acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt
     5761 gatctagtca tcatgaagga aaaaagccct tcatgcggaa cccattcgat ttatgacgga
     5821 agtttttccg gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt tattcagcaa
     5881 aaaatgactg tgctctctga tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat
     5941 cgaaaagacc aatcgaatga acgcgctctt tgaattttat tcgaccttat tgacagaaaa
     6001 acaaatgaac tacatggaat tatattatgc agatgatttt tcattaggtg agatcgcgga
     6061 agaatacgaa gtaagccgac aagcagtata tgacaatatc aagcggacaa gcaagatctt
     6121 ggaggattat gaaaaaaaac tccatctttt ttctaattat attgttcgag aacaggtatt
     6181 ggaaaatatg actgcatata ttacagataa gtacccggaa gataaaaaac tacttgaata
     6241 tgtacagcaa attcaagcaa tcgaagaata aataaaagga atgaagaaaa tatggcattt
     6301 gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta
     6361 tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact agaagccgat
     6421 gttaacttgc aagtagtcaa agattttact aagcgcgtac gcgaacgtgc ggtaggggca
     6481 gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa aaatcgttga tgaagaattg
     6541 acgatcacac ttggttctga aacagcagag ttaaataaat caccaaaaat accaactgtt
     6601 atcatgatgg ctggtcttca aggggcaggt aaaacgacct ttactggtaa attagccaat
     6661 tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt atatcgacca
     6721 gctgcgatcg accaattgaa agtattaggt caacagctag acgttcctgt atttgatatg
     6781 ggaactgaag tcagcccggt agagatcgtc cgtcaaggga tggagttagc aaaagaaaag
     6841 aaaaacgact atgtattgat cgatacggca ggacgtctgc atatcgatga aacattgatg
     6901 gatgagttga aacaaatcaa agaattgacg caaccaaatg agattttgct tgttgtcgat
     6961 gccatgactg gtcaagatgc cgtcaacgta gctgatagct tcaaccagca attaggtatt
     7021 acaggggtcg tcatcactaa acttgatggg gatactcgag gcggggcagc actatcgatc
     7081 cgctcagtga caggcgcacc aatcaaattc atcggttctg gtgaaaaatt gactgactta
     7141 gaagtattcc acccagatcg tatggctagc cgtatccttg gtatggggga tatgctgaca
     7201 ttgattgaaa aagcgcagca agactacgac gagaaaaaag cggaagaact tgcacagaaa
     7261 atgcgggaaa attcattcga tttcaatgac tttattgaac agcttgatca agtgatgggt
     7321 atgggcccac tggaagatct aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc
     7381 gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg
     7441 acgccagcag aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct
     7501 gccggttctg gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt caaggaatca
     7561 agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg
     7621 tttggttcag gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa
     7681 aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg tacagactga
     7741 aaatgtctac tgccggagct tttctatcag caatttttat tctttactaa tacaattact
     7801 ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg
     7861 caatatcatg atactgtctg gggtgttcct gaatatgatg accaaaagct gttccgaaaa
     7921 ttgatgcttg atattaatca agccggattg agctggcaga cgattttaaa taaaagtgaa
     7981 gcattcgatg aagcatatga tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca
     8041 aaaatcagtg aactaatgga aaacaaagga attattcgta accgaagaaa aatcgaagca
     8101 gccgtccaaa acgccaaagc agtcttgaaa attcaagaag aaatcggcag tttttcagag
     8161 tatctttggt cgttttctaa tggaaaaatc gttgattctt cttatcattc acaagaagaa
     8221 gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag atctaaagaa aagaggattt
     8281 aaatttatcg gaagcacaac aatctatgct tttttagaag ctgtcgggat catcaatgat
     8341 caccttgaca gttgtttccg caaagaagag gtcgtacgta tcggcagaaa gggcgaataa
     8401 agcatgcgaa aaatcatatt ttatggtgct ataagtttag atggctattt ggcaacgaaa
     8461 acggatgatc ttcaatggct ttttgacact ccaacaggta agcaaactac ttatgaagct
     8521 ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa
     8581 tatttggaga ccgaagcaat ttatccagag aaaaagaatt tcgttttttc cacccacgca
     8641 caattacaat tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat
     8701 ctacaaatga cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg
     8761 ctattggaac atcaaatgat tgatgaatgg tatatccaga ttacccctgt attattaggg
     8821 gacgggattc ctttattcca aaaaggaaca tatgaagcgc gttttgaaat gctagacacg
     8881 acacgattcg gtgaatttat cgaattgcat tatgtacgaa agtagaagta aataaaataa
     8941 aaaaggcaac tatatgcaga aatgtcctgt acatagttgc cttttttatt accgttatgc
     9001 tttttactct gttaatttgt tagaaaaatc tagatacgca tctaaaatcg tttggaagaa
     9061 acttaatgtt ccttcaacga tattaccatt ttcatcaatt agattcgcaa cgtttccgat
     9121 atatgcttcc ggttgctgca aggttggcac attcaaaaag acaagtgatt ggcgtagatg
     9181 gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca gtaacgacta aacctggttt
     9241 atgatcccaa acactgtgtc cataaggtct tgagcctaca tctaaagcat ttttcaatac
     9301 tgctggcaca gaacggttgt attcaggagt aacgaaaacg acaccgtcta gtcccctcac
     9361 ttcctcacga aaacgagtcc attcagcagg agcctgttct ggagtttcga gatcctcatt
     9421 gtaaaatggc agatcatcga tttttacaaa tacactttcg tacccttcag gaagcaattt
     9481 cgcaaaagct tctgctactt ttttgttgta agaatctttt cttaaactac caataaaaaa
     9541 tccaattttt tttgtcatac gatttttcct ccttcaaact atctataata atcttacaaa
     9601 agataagtaa ctggagcaaa taaaaacact tactgacaaa agctacatag aagatagatt
     9661 acttattttt aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa atcgaattta
     9721 atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca ctacatcttg
     9781 tgaataaaac aatggaggtg gaaataaata atggcagtta aaatccgttt aaaacgtatg
     9841 ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat
     9901 ggacgtttca tcgaaactgt aggtacttac aatcctttga aagaccctgc agaagtagtt
     9961 ttaaaagaag acttagttct tgactggtta tcaaaaggtg cacaaccttc tgacactgta
    10021 cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc acgaagctaa attctcaaag
    10081 aaataaggtg accggatatg aaagatttaa gcgacttagt cttaacaatc gttcgtccgc
    10141 tagtcactta tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt
    10201 acaatctaac agtggctcct gaagatattg gccgtatcat tggaaaacaa gggcgtgttg
    10261 ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt
    10321 tgaatatcat cgatgataag gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc
    10381 agcttttttg ttttttttat taattgaaga aatgattatt tggcaaaaca agcaagaaag
    10441 cgtatcctta taggaggagg gagtgaacag ccaatgagaa agaaaatgtt atttattcca
    10501 tttgctctat tgttaagcgc ttgtagttcg gttactcaag aatcagacaa tacacatcta
    10561 tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc
    10621 accactgaac aaacaatggc tacctcaaat aatgagaaaa agactgcttt agaccagttg
    10681 aaagaacaac agccaaatgt tcccatgcct ctagatgtac cagtctcttc aggctatctc
    10741 aatatcgcag cgacacatac aaagcaaggt tattctattt tatattacag aacagatcgt
    10801 cccctcgggt tgaatgctga cgaattgaat caagaaacgc ctattgcaac ctatctatat
    10861 caatatgggt ttgcttctag tcaagaaaca atccaagttt tgcagccatt tgagatcgac
    10921 acaaatggtc agcaagtgga cttaggttct cgaatcacag ggtatcagca aggtgcagca
    10981 ggttcaagtt ttcttgaatg gcaagaagga aactggcgta ttcgaatccg tgggaataat
    11041 atagaaggcc aagatccctt actattagct aaagaaatcg tagcttattt ggaagaaaac
    11101 agtctaccag cacctgaaca atttggtaaa attactgttg atatgggtga tacgacgaat
    11161 cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct atacgatcac tcatcaagat
    11221 cctatgtctg cgttgaagat ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat
    11281 ctgagcccat catttaactc caagtcacag gaagtaatca aaaatagcac gccgcatttt
    11341 tgcataattt tggcttaata ccttaaaaga aaactagaat tgcctcttca cacatgctaa
    11401 aataaaacag acaaaaaaga aatggggata taagttgact gaatacttga atgtaggaaa
    11461 aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt atctcaacaa cggatttccc
    11521 agaagaacgt tacaaaaaag gaacggtgct aacactattc caagacggaa aagcacctgt
    11581 ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt gacttattga gttttgaagg
    11641 acatccttct atcaatgatg tggaaaaata tcgggatggc atattaaaag tgtcaaaaga
    11701 caactctgta gatctagctg aaaatgaatt ctattaccat gagatcatcg gtctgcatgt
    11761 attagaagat ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga
    11821 tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg cttccatata tcgcttctgt
    11881 cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta gaaattccag aaggactgat
    11941 tgacgatgaa gattgatgta ttgactttat ttccaagaat gtttgaaggt cctatggggg
    12001 aatccatcat cggtaaggct gtagataaag gattattaga tatcaatatc tctaacttcc
    12061 gcgattattc agataacaaa catcagacag tagatgatta tccatacggc ggtggagcag
    12121 gtatgctgct caaagtccag cccatctatg ataacatcca agccattgaa aaagctgcac
    12181 cggatgtcaa aaaaagagta attcttcttg acccagctgg taaacgattc gatcagaaaa
    12241 tggcagaaga attttcacaa gaagaacact tagtattcat ttgtggtcat tatgagggat
    12301 atgatgaacg tatccgcaca ttagtgacag atgaagtttc cttaggagat tatgtcctta
    12361 ctgggggaga attaggtgcg atggtcatga tcgatgcaac agttcggctt ttgccagatg
    12421 tgttagggaa tcagacttca gctcaaacgg attcttattc aaccggttta ttggaacatc
    12481 cccaatatac tcgcccggca gaatataaag ggatgaaagt accagaagta ttgacaaatg
    12541 gaaatcataa attgattgaa gaatggcaat taaaagaatc actaagaagg acctatcaac
    12601 gtcggcctga tcttttggaa acacttgaat ggacacctca aatgacgaaa tttttagaag
    12661 aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc caagtgactt tttctaaaat
    12721 cttctttaca gtaggttgat aatatgttag tattttatgg tgagtgcaaa agcacttaac
    12781 tattacgata ttccgctgtg agaaaagctc ataagaatat ttggaataag gagaagaaaa
    12841 ccatgaatcc attaatcgaa gaattaacaa aagaacaatt acgttctgac atcccagcgt
    12901 tccgccctgg tgacactgtg cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta
    12961 tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc gaaacttata
    13021 cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac attcccattg catacaccac
    13081 gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc
    13141 tacgtgcatt acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa tcatcgttat
    13201 cagactgaga aagaaccctt ggtattcaag ggttcttttt ttgttctctg tttttttata
    13261 ttaacgattt ctatttggga catttttgat taataaaaag gggcaaaaaa ggggcaacaa
    13321 gttacagttt ttctatatct tctttcaatt tttcttttac tccctttgtc acatgcaaat
    13381 aaatcttttc agtgatatca ctattttcgt gcccaacgcg atcctgaata gcatacagag
    13441 gagttcctag ttccgctaat tttgaaatat gggtatgtct aaaaatatgt gaactaagtt
    13501 ttttatcaat cttcatgtca gctttatgat ttctcaaata ggtgttaatt gctgttagtt
    13561 ggaaaggagt tcctttcgtt gtttgaaata gaaattgacc attcggattt aattctagaa
    13621 gctcattgta aatagctatc gctttttttg gtaaatcaat ttctcgcatt ccagcagcag
    13681 tttttgtaga atcagatttt ttcatatcag ctattgaacg ttctctatac atcatcgtcc
    13741 catttataac tactgatgca ttattattgg taatgtgtac atcgtcttta cttaatgcga
    13801 ttgcttctcc aggcctcatt ccagtcaaat atagccactg gaaaaggaga gaatatcttt
    13861 tattgtgtga agttgtgaaa tttactagtc ttttatattc atcatcttct aaaaacttat
    13921 ctttaatttt tatcgttttt gactctcgtt tataatcaat aactacttcg ttgataggat
    13981 ttttttctac atagcctttt ttcatagcgt atgaaaaaag aagattcaat ttggatttaa
    14041 ttacactgac gtacttattt gacaagtcat ctttatatat catatcttca aaaatattat
    14101 ttaaatctac tgtattaata ccagaaacga tgtaagtctc aggtatcttt ttctttatag
    14161 tgtttaagat attattcgta ggataatatg tagactcctt aacttgtctt ttgtaaataa
    14221 ctaaccattc ttcgacaagc tcatgaaatg ttagatcagg cttctgaagt gttttttcgt
    14281 ttaattttat atcaattttt ttatttaact ctagcaatgc cactttctga gtttctcgag
    14341 atttattttt ataagtaatg ctcacttttt ttctttttct agtttttgga tcaatatatc
    14401 gctcattata tttatatact ttttttccgt ttttatcagt tttggtttca atccacattg
    14461 ttttcatctc ctatttgttg ctataatagg aatggataag taagccaatt atagcaggtt
    14521 tatttttcat cacgtccaca aactttggcg agggaggggg cgtgtttttt tgtttagtag
    14581 ctacctttcc atgaagtatc tgcgaagaac acttcaaatg ttgttccatc atttttcact
    14641 tcaaaatatg ctgttccttg tgcggattta cccggttgga tggtttccga aaagtaatct
    14701 ctactagcta aatcagattt aatatctgat gaatcataaa attctaaata acttgaactt
    14761 gcatcaaaag gtgtagtccc tgtgttttct acaagaaaat ctacctttgc gtaaaatagg
    14821 ccttctggtt tatagaaatc atcaccgtaa tcttttgata cagactggac agttacgtta
    14881 atagagttac cattttcaga agtaaaagga actgcttctc ctatactatg tgcaactgat
    14941 gccgtctggg gttctgaaga cgaagttgta gttgtagttg tagtagagct tgaatctttg
    15001 gattttccat ctttatctaa gaaagttcgt tgattataat atacaacctg ttcagttttt
    15061 ggactaaccc aaattaacat tgtttcatca tcagaatcag ttgaataagt atattggagc
    15121 atctctaatt tttttatatc ttttgcacta tttccatgtt caattaattc agagtgctct
    15181 ttataataat ctccttgtgc atcctttgaa atagatacaa ttaattcatc ccgtgtgata
    15241 gcattgtcta attctttgta aacaaaatca tttgtccatt tacgtaacgg tgaaccaagt
    15301 gtctgataaa cttgagttaa atcatcacca acttttattt tatgaacgtc tgatgaagtt
    15361 agtttttttg tttcatattc agatgttgat gaaacagtat tttctttagt gctagcagaa
    15421 ttaggactag tggagcatcc gcttaatacc aagcctaaaa aaagcaaccc aaaaacaatt
    15481 tttttcattt ttaattcctc acttctatga tatgattttt ttgtaggatc ttagaaataa
    15541 ggtttttagt ccgtgttgca gcacggactt tttttacatt atataagaaa gcgataagcg
    15601 ctttctggaa gtccgtaaag attctttaat tcctcaattc gtttaggata ttgatcattg
    15661 tcttctttgt atagagaaac aatgagatta gcagcaaagc aattagcttc gctttcagat
    15721 ttgcttctag atgttcttgt tgatacatag taactggata agccacgatg aaaaatagcg
    15781 tgacctaatt cgtgagcgca aatgtagaat ctttcctcag agtctctcag ttcatcattt
    15841 aagaagatta ttgcacgacc tctaatttct tgaaactgtc c
//
LOCUS       DAZKAS010000057        15687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000057 DAZKAS010000000
VERSION     DAZKAS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.058"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACG8H7_001530"
     CDS             68..751
                     /locus_tag="ACG8H7_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFP8983629.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACG8H7_001531"
     CDS             753..1589
                     /locus_tag="ACG8H7_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP8983630.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACG8H7_001532"
     CDS             1600..3354
                     /locus_tag="ACG8H7_001532"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983631.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACG8H7_001533"
     CDS             complement(3630..3971)
                     /locus_tag="ACG8H7_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFP8983632.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACG8H7_001534"
     CDS             complement(4039..6210)
                     /locus_tag="ACG8H7_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFP8983633.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACG8H7_001535"
     CDS             6421..7278
                     /locus_tag="ACG8H7_001535"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFP8983634.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACG8H7_001536"
     CDS             complement(7349..8206)
                     /locus_tag="ACG8H7_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8983635.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACG8H7_001537"
     CDS             complement(8191..10893)
                     /locus_tag="ACG8H7_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP8983636.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACG8H7_001538"
     CDS             complement(10906..12195)
                     /locus_tag="ACG8H7_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFP8983637.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACG8H7_001539"
     CDS             12345..13391
                     /locus_tag="ACG8H7_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8983638.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACG8H7_001540"
     CDS             13393..15546
                     /locus_tag="ACG8H7_001540"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFP8983639.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4454 a         3282 c         2887 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DAZKAS010000058        15585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000058 DAZKAS010000000
VERSION     DAZKAS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.059"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..791)
                     /locus_tag="ACG8H7_001541"
     CDS             complement(72..791)
                     /locus_tag="ACG8H7_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8983640.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(922..2073)
                     /locus_tag="ACG8H7_001542"
     CDS             complement(922..2073)
                     /locus_tag="ACG8H7_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFP8983641.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2290..3030)
                     /locus_tag="ACG8H7_001543"
     CDS             complement(2290..3030)
                     /locus_tag="ACG8H7_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8983642.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3034..3357)
                     /gene="rsfS"
                     /locus_tag="ACG8H7_001544"
     CDS             complement(3034..3357)
                     /gene="rsfS"
                     /locus_tag="ACG8H7_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP8983643.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3414..4007)
                     /gene="yqeK"
                     /locus_tag="ACG8H7_001545"
     CDS             complement(3414..4007)
                     /gene="yqeK"
                     /locus_tag="ACG8H7_001545"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFP8983644.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3991..4641)
                     /locus_tag="ACG8H7_001546"
     CDS             complement(3991..4641)
                     /locus_tag="ACG8H7_001546"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP8983645.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4651..4962)
                     /gene="yhbY"
                     /locus_tag="ACG8H7_001547"
     CDS             complement(4651..4962)
                     /gene="yhbY"
                     /locus_tag="ACG8H7_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP8983646.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4978..6087)
                     /gene="yqeH"
                     /locus_tag="ACG8H7_001548"
     CDS             complement(4978..6087)
                     /gene="yqeH"
                     /locus_tag="ACG8H7_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFP8983647.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6084..6611)
                     /locus_tag="ACG8H7_001549"
     CDS             complement(6084..6611)
                     /locus_tag="ACG8H7_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFP8983648.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6791..7576)
                     /locus_tag="ACG8H7_001550"
     CDS             complement(6791..7576)
                     /locus_tag="ACG8H7_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP8983649.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7569..8438)
                     /gene="accD"
                     /locus_tag="ACG8H7_001551"
     CDS             complement(7569..8438)
                     /gene="accD"
                     /locus_tag="ACG8H7_001551"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP8983650.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8439..9818)
                     /locus_tag="ACG8H7_001552"
     CDS             complement(8439..9818)
                     /locus_tag="ACG8H7_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP8983651.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9826..10245)
                     /gene="fabZ"
                     /locus_tag="ACG8H7_001553"
     CDS             complement(9826..10245)
                     /gene="fabZ"
                     /locus_tag="ACG8H7_001553"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP8983652.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10270..10746)
                     /gene="accB"
                     /locus_tag="ACG8H7_001554"
     CDS             complement(10270..10746)
                     /gene="accB"
                     /locus_tag="ACG8H7_001554"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP8983653.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10749..11984)
                     /gene="fabF"
                     /locus_tag="ACG8H7_001555"
     CDS             complement(10749..11984)
                     /gene="fabF"
                     /locus_tag="ACG8H7_001555"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP8983654.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12003..12740)
                     /gene="fabG"
                     /locus_tag="ACG8H7_001556"
     CDS             complement(12003..12740)
                     /gene="fabG"
                     /locus_tag="ACG8H7_001556"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFP8983655.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12752..13669)
                     /gene="fabD"
                     /locus_tag="ACG8H7_001557"
     CDS             complement(12752..13669)
                     /gene="fabD"
                     /locus_tag="ACG8H7_001557"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP8983656.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13776..14000)
                     /locus_tag="ACG8H7_001558"
     CDS             complement(13776..14000)
                     /locus_tag="ACG8H7_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP8983657.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14053..15015)
                     /locus_tag="ACG8H7_001559"
     CDS             complement(14053..15015)
                     /locus_tag="ACG8H7_001559"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP8983658.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15025..15465)
                     /locus_tag="ACG8H7_001560"
     CDS             complement(15025..15465)
                     /locus_tag="ACG8H7_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8983659.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4080 a         3555 c         2678 g         5272 t
ORIGIN      
        1 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
       61 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
      121 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
      181 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
      241 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
      301 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
      361 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
      421 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
      481 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
      541 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
      601 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
      661 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
      721 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
      781 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
      841 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
      901 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
      961 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
     1021 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
     1081 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
     1141 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
     1201 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
     1261 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
     1321 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
     1381 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
     1441 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
     1501 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
     1561 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
     1621 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
     1681 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
     1741 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
     1801 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
     1861 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
     1921 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
     1981 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
     2041 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
     2101 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
     2161 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
     2221 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
     2281 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
     2341 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
     2401 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
     2461 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
     2521 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
     2581 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
     2641 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
     2701 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
     2761 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
     2821 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
     2881 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
     2941 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
     3001 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
     3061 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
     3121 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
     3181 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
     3241 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
     3301 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
     3361 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
     3421 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
     3481 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
     3541 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
     3601 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
     3661 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
     3721 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
     3781 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
     3841 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
     3901 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
     3961 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
     4021 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
     4081 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
     4141 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
     4201 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
     4261 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
     4321 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
     4381 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
     4441 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
     4501 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
     4561 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
     4621 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
     4681 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
     4741 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
     4801 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
     4861 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
     4921 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
     4981 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
     5041 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
     5101 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
     5161 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
     5221 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
     5281 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
     5341 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
     5401 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
     5461 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
     5521 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
     5581 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
     5641 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
     5701 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
     5761 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
     5821 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
     5881 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
     5941 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
     6001 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
     6061 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
     6121 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
     6181 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
     6241 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
     6301 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
     6361 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
     6421 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
     6481 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
     6541 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
     6601 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
     6661 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
     6721 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
     6781 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
     6841 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
     6901 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
     6961 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
     7021 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
     7081 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
     7141 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
     7201 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
     7261 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
     7321 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
     7381 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
     7441 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
     7501 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
     7561 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
     7621 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
     7681 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
     7741 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
     7801 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
     7861 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
     7921 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
     7981 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
     8041 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
     8101 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
     8161 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
     8221 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
     8281 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
     8341 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
     8401 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
     8461 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
     8521 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
     8581 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
     8641 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
     8701 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
     8761 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
     8821 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
     8881 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
     8941 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
     9001 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
     9061 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
     9121 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
     9181 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
     9241 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
     9301 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
     9361 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
     9421 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
     9481 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
     9541 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
     9601 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
     9661 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
     9721 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
     9781 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
     9841 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
     9901 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
     9961 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    10021 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    10081 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    10141 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    10201 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    10261 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    10321 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    10381 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    10441 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    10501 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    10561 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    10621 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    10681 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    10741 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    10801 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    10861 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    10921 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    10981 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    11041 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    11101 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    11161 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    11221 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    11281 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    11341 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    11401 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    11461 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    11521 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    11581 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    11641 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    11701 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    11761 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    11821 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    11881 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    11941 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    12001 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    12061 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    12121 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    12181 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    12241 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    12301 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    12361 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    12421 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    12481 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    12541 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    12601 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    12661 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    12721 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    12781 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    12841 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    12901 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    12961 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    13021 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    13081 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    13141 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    13201 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    13261 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    13321 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    13381 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    13441 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    13501 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    13561 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    13621 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    13681 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    13741 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    13801 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    13861 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    13921 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    13981 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    14041 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    14101 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    14161 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    14221 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    14281 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    14341 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    14401 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    14461 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    14521 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    14581 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    14641 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    14701 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    14761 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    14821 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    14881 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    14941 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    15001 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    15061 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    15121 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    15181 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    15241 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    15301 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    15361 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    15421 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    15481 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    15541 caaccttaaa agatcatttt tttactatat gagctttttt taaga
//
LOCUS       DAZKAS010000059        15230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000059 DAZKAS010000000
VERSION     DAZKAS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.061"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8H7_001561"
     CDS             278..787
                     /locus_tag="ACG8H7_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8983660.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8H7_001562"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8H7_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8983661.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG8H7_001563"
     CDS             2322..3302
                     /locus_tag="ACG8H7_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8983662.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG8H7_001564"
     CDS             3424..4842
                     /locus_tag="ACG8H7_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFP8983663.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG8H7_001565"
     CDS             4913..6382
                     /locus_tag="ACG8H7_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8983664.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG8H7_001566"
     CDS             6392..6859
                     /locus_tag="ACG8H7_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP8983665.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8H7_001567"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG8H7_001567"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP8983666.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="ACG8H7_001568"
     CDS             7848..10382
                     /locus_tag="ACG8H7_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8983667.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="ACG8H7_001569"
     CDS             10538..11311
                     /locus_tag="ACG8H7_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8983668.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="ACG8H7_001570"
     CDS             11304..12095
                     /locus_tag="ACG8H7_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8983669.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="ACG8H7_001571"
     CDS             12105..12596
                     /locus_tag="ACG8H7_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP8983670.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="ACG8H7_001572"
     CDS             12614..13024
                     /locus_tag="ACG8H7_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8983671.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="ACG8H7_001573"
     CDS             13028..14428
                     /locus_tag="ACG8H7_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFP8983672.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="ACG8H7_001574"
     CDS             14451..14825
                     /locus_tag="ACG8H7_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8983673.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2135 c         3045 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcatttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAZKAS010000060        15144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000060 DAZKAS010000000
VERSION     DAZKAS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.062"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..482)
                     /gene="yajC"
                     /locus_tag="ACG8H7_001575"
     CDS             complement(102..482)
                     /gene="yajC"
                     /locus_tag="ACG8H7_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP8983674.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(579..1724)
                     /gene="tgt"
                     /locus_tag="ACG8H7_001576"
     CDS             complement(579..1724)
                     /gene="tgt"
                     /locus_tag="ACG8H7_001576"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP8983675.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1942..2640
                     /locus_tag="ACG8H7_001577"
     CDS             1942..2640
                     /locus_tag="ACG8H7_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP8983676.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2808..5447)
                     /locus_tag="ACG8H7_001578"
     CDS             complement(2808..5447)
                     /locus_tag="ACG8H7_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP8983677.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6066..7097)
                     /gene="queA"
                     /locus_tag="ACG8H7_001579"
     CDS             complement(6066..7097)
                     /gene="queA"
                     /locus_tag="ACG8H7_001579"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP8983678.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7290..8465
                     /locus_tag="ACG8H7_001580"
     CDS             7290..8465
                     /locus_tag="ACG8H7_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP8983679.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8469..9110
                     /locus_tag="ACG8H7_001581"
     CDS             8469..9110
                     /locus_tag="ACG8H7_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983680.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9107..10024
                     /locus_tag="ACG8H7_001582"
     CDS             9107..10024
                     /locus_tag="ACG8H7_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8983681.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10021..10692
                     /locus_tag="ACG8H7_001583"
     CDS             10021..10692
                     /locus_tag="ACG8H7_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983682.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11233..11721)
                     /locus_tag="ACG8H7_001584"
     CDS             complement(11233..11721)
                     /locus_tag="ACG8H7_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFP8983683.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11922..12188)
                     /locus_tag="ACG8H7_001585"
     CDS             complement(11922..12188)
                     /locus_tag="ACG8H7_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP8983684.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12188..12451)
                     /locus_tag="ACG8H7_001586"
     CDS             complement(12188..12451)
                     /locus_tag="ACG8H7_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP8983685.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12972..13112
                     /locus_tag="ACG8H7_001587"
                     /pseudo
     CDS             12972..13112
                     /locus_tag="ACG8H7_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13254..15077)
                     /locus_tag="ACG8H7_001588"
     CDS             complement(13254..15077)
                     /locus_tag="ACG8H7_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP8983686.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4391 a         3244 c         2855 g         4654 t
ORIGIN      
        1 attgagcaat atatcccatg ctcttatctc tttctttttt gaataaaaaa tgaggtagga
       61 catctttctg ccctacctcg tctttaaata agctttgata cttatttttc ttccttctct
      121 tctgtctctt ccagcgtttc tgttgtttca gtttttactt caactgtctc agctggttct
      181 tctgttgttt caacagtagt cgttgcatca tttgttacga cagtccctgg tttgaccgtt
      241 ttgattgctg cacgatcgta ctcaaggaag atcccttcgc agtctaaaac aactgtccgt
      301 ttgtcattgt cgatttctga aacaactcca tgtaatccgc cgatcgttac gactgaatca
      361 cctggtttca tgctatctaa caagttttga cgttcttgct gttgtttctt ttgcgaacgt
      421 gacataaaaa accacattgc taatagcgcg ataaacatca caagcattgg taatgttccc
      481 atttttttca cctcactaat tctgtatcta caaaaaacac tttagcagaa aaatgctgat
      541 aacactagtt ttttacttaa tttttaaaag attttccttt agaagctttt tgcgttttct
      601 ttattgaacc cgtattcttc aaaaaaggct tctctaaatt caagcaaatt atcatccatg
      661 attgcttgac gtacttgttt cattagattc aacaagaagt ataaattgtg gtaagaagtc
      721 aaacgaatcc caaatgtttc atcacatttg atcaaatggc ggatataggc acgtgtatag
      781 tttttacatg tataacaatc acatttctca tcaattggac ggaaatcatg ggcatatttc
      841 gcatttttca ctactagacg gccttgagaa gtcatacatg tgccatttcg agcgattcga
      901 gtaggcaaga cacagtcaaa catgtctact ccacggataa caccatcgat caatgaatca
      961 gcagcaccga cacccatcaa gtaacgtggt ttgttttcgg ggatcaacgg tgtagtgaat
     1021 tctaatacac gattcatttc tgatttaggt tccccgacag ataacccgcc gattgagtaa
     1081 ccagggaaat ccatcccgac tagatctttc gcgctttgac gacgaagatc ttcaaaccct
     1141 gctccttgga tgattccaaa taatccttga cggtctggat tagcatgagc tttcaatcct
     1201 cgttctgccc atcgagacgt acgttctact gattttttca cgtagtcgta actttcatcg
     1261 aatggtggac attcgtcaaa actcatcatg atatcagagc ccaatttatt ttggatgttg
     1321 attgcttttt ctggtgataa aaacatacgc gaaccgttca agtgattttt gaaatgcacg
     1381 ccttcttctt cgatattccg catatcactt aatgaaaaaa cttgaaagcc gcctgaatct
     1441 gttaaaatcg gttgatccca gttcataaat ttatgcagtc cgccagcttc ttctactaag
     1501 tcttcaccag ggcgtagcca taaatgatat gtattactta aaataacgcc agcacccatt
     1561 tctttcaact cttctgggga cattgttttt actgtagcta atgttcctac tggcatgaac
     1621 attggtgtgg ggaaagttcc gtgtggcgtg atcaattccc ctaaacgcgc gccagtatgt
     1681 ttttctttct tgattaaacg atagcgaatg gcaggttgcg tcattcattt ccctactttc
     1741 tatataaaat atcttgacaa ctcccaccat cataatcgtt tcaagtgctt tttgcaagtt
     1801 ccctcggctt ccttctactt tatttaatat tctttttttt atcctttttt tatgaataac
     1861 aaaaaaaaga aaaacattat gacagaaata ttttttgata catggtatat tggtgttatt
     1921 aaagaaaagg agggaaaaat catgcaatct tctgcagaat taaaacgcca agcaaaagag
     1981 agtctaaaag gacgttgggg acaagctgtt ttactaaatc taatccctac attgattggt
     2041 attgctacta ttcttcttat tatgattcct attatcttgt ttgccaccac catatatgat
     2101 ggatcagcga gcgtctcaga agcgacaact tcaggaggcg gtagttcagg aggcggtggc
     2161 ggaattattt ccagcatcat tagcgctttg ttcatgagcg gtatttcttg gacttatctt
     2221 gatattttac gtggacaaaa agatcagatc gaaccattca aagatgcatt tcgtggattc
     2281 aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat ctttacaaca
     2341 ttatggggtt tgctattagt tattcctgga atcattaaaa gctattctta ttctcaaagc
     2401 tattttattt actacgatat cgtccaagaa acaggacaaa cacctaaagt actggatacg
     2461 atcactgcaa gtcgaaaatt gatggatggt tataaaggac aacttttctg gttagatctt
     2521 tcatttatcg gctggcatat cttagcatta gcaactgctg gtattggtta tctatggctc
     2581 agcccataca tttctgcaac aaaagcagca ttttatgaac aattgccaaa agatatttaa
     2641 aagagagtgt gacttgtgac cgagggggtg gcaggagaac accgtttatc gagaggcagt
     2701 aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac aacattcatg tcacacctcc
     2761 tttttgtata gttccagtaa aaagaaaatc aacttagaaa cagttaatca ttcctcttct
     2821 tctgcttccg gtaatcgatg gaacttgatc agttcattca ccgcgattgg tacgatagat
     2881 aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc
     2941 gtaaatggca gcaaagcaac agccgttgta atagctaatg ccagtaacgc catttctacc
     3001 aaagctttat tggtcgctaa attgactttg aagatcgatt ggctgctgcg tacattgaac
     3061 acatgcagaa tcgatgaata agctaagatc aagaatgcaa cagtttgacc gacatgctga
     3121 cttgcgtcta cccatgggct gatagtatcg acataactac ctaaataata accaaacaag
     3181 gttacgatgg tgaagacccc tgcatttatg gcaatttttt gcaataaacc tcgagcaaag
     3241 atcccttcat ttttcggaat gggtggctga tccatgatgc cgtgttccgc tggctctctt
     3301 cctaaagcaa atcctggaag tccatcagca actacattga caaacagtag ttgcacagct
     3361 gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa caataaagat ctccgaaata
     3421 ttacagctta gcaaaaagtt gatagcttta cgaatattct gataaaccga acgtccttga
     3481 ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc tgcggcactt
     3541 tgcgccacat ctgttcctgt gatccccatg gcacacccaa cgttacttgc tttcaacgct
     3601 ggtgcatcat tcacaccatc cccagtcatc gctacgactg cacccgtacg ttgccaagat
     3661 ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat agacagagag agctttgaca
     3721 cgcgcatcta attcttcgtc tgataattga tgcagttctg atcctgaaag tgcttctgac
     3781 ggatcattca agatacctaa ttctttcgct atggcactgg cagtcaccac atgatctcca
     3841 gtgatcatga ctgttttgat gccagccttt ttcgctcgct tgattgctcc tttactttct
     3901 ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct
     3961 tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat aaccaacagc aatcactctc
     4021 agtgcccgtt taccaaagcg atcattaacg atcgccgctt gttctacatc gcctgaacta
     4081 aagcgcggag caagaacatc gaatgctcct ttggtgattg agatgtattt ttcgcccatt
     4141 tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga
     4201 tatttttctt ctaattctgc ctttgtatga taattctctt caacagccct tacgatggct
     4261 acttctgttg gattaccttt gataacaagg tcgtcatctt cttgctcgac cgtcacatct
     4321 gtacatagtg cagccatttt caatatttcc atcgcgctgt tcgtcattga atcttcgata
     4381 ttcgttactt catcttcttt gctccatacc cggcggacgc gcatttcatt ttgtgtcagc
     4441 gttcctgttt tgtctgagca gataacatta gcggttccta gcgtttcaac tgctggcaac
     4501 tgtcggatga tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta
     4561 acaataacag tcaaggtttc cggtacagct gcaacagcca aggaaatcga agtcatgaac
     4621 atctcaagga gtggttctcc ttgcagttca ccaatcacaa atactaaagc cgcagccgct
     4681 aaagcgatca tactgattcg tttgcctagc tgattcaatc ggacctgtaa aggtgttttt
     4741 tgcatagtat tttcgttcaa aagatccgcg atcttaccca tttctgtagc catgccaatg
     4801 gcagtgacga tcgctttccc acgacctgca gcaaccgtac atcctttaaa taccatatta
     4861 agttggtcac ctaatgactc atcttcttca gcaataaatg ctgcatcttt ttcaactgct
     4921 tcactttctc cagtcaacgc cgattcttct acacgtaatt gtgatgcttc gatcaaacgg
     4981 gcatcggcag aaatagcatc accggactct aaaacaagaa tatcacccac taccagttgc
     5041 tctgactcaa cagtggtcac tttgccgtct cgtatgacag ttgccgtttg tttattcata
     5101 tcttgcaagg attccagtgc tttttctgcg tttccttctt ggacaatcgc caataccgag
     5161 ttgatcacaa cgatagcgat gatcagcagt ccttcaaata aatccccatg ttctgtagca
     5221 aatgcggtat aaaaggaaat caccgctgct acaagaagga tgatcgttgt aaaatcggac
     5281 aagcttcgaa agaatttttt gatcatcgat tctttcggcg cctcatcgaa tttattcaat
     5341 ccctgctttt ttcgccgttc atccacttct tgtgaagaca acccagtatc tggatctgct
     5401 tctgttacct tctttacttc ttctattgtc tgcttgtatt cttccatcct attcctcctt
     5461 taaaatcttc gtacaaaaaa aggtacttat tttactataa catccttttt ttatttactt
     5521 caaataaagg gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt gcttatattt
     5581 gcaaacgcta ccaaaggatg ctattatata gatgtaagga aggaggatac catcaaatag
     5641 acagaagaat ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc
     5701 acccagacat gcgtgtagag tacaaaccta taaatagtga atgcgtatgg atgtttggat
     5761 cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca attgaataac gtaaacgtct
     5821 gaaaattgaa taggaacaag agatgtttca aatgttgaag atgtaacgaa aacaaaattt
     5881 ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa gttacaatca
     5941 tttctgttta tttgaatcct tccgaggtac aagaaagccg agcttatcga gagctcggct
     6001 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
     6061 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
     6121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
     6181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
     6241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
     6301 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
     6361 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
     6421 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
     6481 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
     6541 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
     6601 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
     6661 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
     6721 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
     6781 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
     6841 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
     6901 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
     6961 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     7021 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     7081 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     7141 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     7201 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     7261 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     7321 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     7381 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     7441 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     7501 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     7561 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     7621 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     7681 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     7741 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     7801 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     7861 ttgtaaccca tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg
     7921 aaggccgtgt gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt
     7981 tcgtagaaga gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag
     8041 tcggcgaagt tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag
     8101 caatcaaact gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg
     8161 tattaaaagg atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg
     8221 tcggcgacat tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata
     8281 ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca
     8341 aagtcgtagg tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg
     8401 gagaagatcc cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg
     8461 tctgacctat gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg
     8521 aacatcttta tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg
     8581 gtgtcttact gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct
     8641 tgcagacaat cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta
     8701 aattacctgc gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt
     8761 acattgggat gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga
     8821 ctgagttcca atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg
     8881 gtattcgttt agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg
     8941 ctggtggttt aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt
     9001 tggcaggtac gatccctgta acaattttag cactattggc tgattggctt ctaggtctct
     9061 tggaacaccg gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact
     9121 aaaaatgacc atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct
     9181 tgccagcaat acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca
     9241 gatcttagca agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat
     9301 catcaacaat ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc
     9361 aatttcagcg gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga
     9421 aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca
     9481 aacgtggttc ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac
     9541 cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt
     9601 agctggtgta gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga
     9661 aacttatggc ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc
     9721 agtcgaagca ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag
     9781 ttatgatcta gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc
     9841 agttgtagac aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt
     9901 aggtcatacc atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct
     9961 agtggaacca tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa
    10021 gtgaagcaat tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat
    10081 ggctcctata tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc
    10141 tttgcagcag ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca
    10201 aactgggtga ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc
    10261 attttaatca ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat
    10321 tccctcttac cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc
    10381 cttgatgtcg gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta
    10441 ccactctctg tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg
    10501 ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga
    10561 accaatgcaa cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg
    10621 gcgatcatca cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt
    10681 acatcacgct gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa
    10741 ggctttttta gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg
    10801 ggtaaagagt tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc
    10861 aacgggttca tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc
    10921 caatccattt tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc
    10981 tgcttgttct ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc
    11041 cttttccatc agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg
    11101 ccgtatcggt aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg
    11161 ctaaaaacat atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc
    11221 ggtacacgat tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt
    11281 caccagaaga gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt
    11341 ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga
    11401 aagcgccacg actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt
    11461 ggatacccgc tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc
    11521 aaaagaccat gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat
    11581 tgcttttttc aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc
    11641 ctcactttgg cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat
    11701 tttactgatt ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc
    11761 cattcctaaa ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa
    11821 tcatgcaata aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga
    11881 gactctttgt taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag
    11941 gcagaataaa tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt
    12001 ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc
    12061 gtgggcatat atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa
    12121 ttcgtttgct tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag
    12181 ttactcatca aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt
    12241 tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa
    12301 tcgctctcca gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa
    12361 tctcgatttc agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag
    12421 cagtgctagg attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta
    12481 tagcgtactt gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt
    12541 ttcctctttt ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca
    12601 cttcttactc ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca
    12661 tctttagcaa aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag
    12721 gttcctcgtt gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag
    12781 atttgtaacg gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg
    12841 attgcctctt tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg
    12901 ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac
    12961 ttgacgaggc tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg
    13021 aagaagctga aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc
    13081 aagacatcaa aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc
    13141 tgttcccatc tttaatggct tatagagggc acctttagct gagaataaga caaaacagac
    13201 cctctagctt caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc
    13261 ttcaaatggt agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt
    13321 aaccgcaaac ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga
    13381 ggaatcaaca cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca
    13441 taacggataa ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca
    13501 tctgggaaat gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct
    13561 agcgaaacat gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg
    13621 atatttaccc gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt
    13681 agttttgcat cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc
    13741 ttataaaaaa tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac
    13801 cgatagacaa acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca
    13861 atcgatttgc ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga
    13921 gataaagcaa atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag
    13981 ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa
    14041 tagcctagtc gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga
    14101 ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc
    14161 gctgtagcaa attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt
    14221 tgagacaaaa cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg
    14281 atccccacga agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt
    14341 tttgctcttg gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa
    14401 gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc
    14461 gctcctggaa gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca
    14521 acaacgatca gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat
    14581 gcagattccc tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa
    14641 atagcgactt gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct
    14701 gagacagata ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag
    14761 ccagcattct ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa
    14821 gcatggatga tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg
    14881 gcaatgggta gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt
    14941 tgttccgtac catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca
    15001 aatcgggtta atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac
    15061 ctttttaagt aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag
    15121 tcgaaaattt atagtaaaca agat
//
LOCUS       DAZKAS010000061        14867 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000061 DAZKAS010000000
VERSION     DAZKAS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.063"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..261)
                     /locus_tag="ACG8H7_001589"
     CDS             complement(64..261)
                     /locus_tag="ACG8H7_001589"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP8983687.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(407..1285)
                     /locus_tag="ACG8H7_001590"
     CDS             complement(407..1285)
                     /locus_tag="ACG8H7_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8983688.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1324..2442)
                     /locus_tag="ACG8H7_001591"
     CDS             complement(1324..2442)
                     /locus_tag="ACG8H7_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFP8983689.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2439..4538)
                     /locus_tag="ACG8H7_001592"
     CDS             complement(2439..4538)
                     /locus_tag="ACG8H7_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFP8983690.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4589..5410)
                     /locus_tag="ACG8H7_001593"
     CDS             complement(4589..5410)
                     /locus_tag="ACG8H7_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8983691.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5407..6216)
                     /locus_tag="ACG8H7_001594"
     CDS             complement(5407..6216)
                     /locus_tag="ACG8H7_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP8983692.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6237..6731)
                     /locus_tag="ACG8H7_001595"
     CDS             complement(6237..6731)
                     /locus_tag="ACG8H7_001595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8983693.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6743..7243)
                     /locus_tag="ACG8H7_001596"
     CDS             complement(6743..7243)
                     /locus_tag="ACG8H7_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8983694.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7308..10004)
                     /locus_tag="ACG8H7_001597"
     CDS             complement(7308..10004)
                     /locus_tag="ACG8H7_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8983695.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10432..11505)
                     /locus_tag="ACG8H7_001598"
     CDS             complement(10432..11505)
                     /locus_tag="ACG8H7_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8983696.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11502..12329)
                     /locus_tag="ACG8H7_001599"
     CDS             complement(11502..12329)
                     /locus_tag="ACG8H7_001599"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983697.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12330..13136)
                     /locus_tag="ACG8H7_001600"
     CDS             complement(12330..13136)
                     /locus_tag="ACG8H7_001600"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983698.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13148..14233)
                     /locus_tag="ACG8H7_001601"
     CDS             complement(13148..14233)
                     /locus_tag="ACG8H7_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983699.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14250..14792)
                     /locus_tag="ACG8H7_001602"
     CDS             complement(14250..14792)
                     /locus_tag="ACG8H7_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP8983700.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         4529 a         3292 c         2434 g         4612 t
ORIGIN      
        1 tttttataat cacaactctc tataatagaa ttacgattta ttttatacgg ctgaaaaaat
       61 ctatcaccaa aaaaattcaa acattaagcc aaggattatg aagtcaacat ttcgaaaaat
      121 gaacatacat tggtaagcaa ctataactat taccacagac ataaagtaac acaatatatt
      181 actatttaag tgtacgccac cagtgaatat cctcaaggta tacagcgcac aacaaaaaga
      241 aaaaaggaaa cgcagaagca tttcctttca gtgatttaat gaaaacaaca cgatttgact
      301 tagagccttt ttatttgaat tcattttcgc tttatccaac ttcatttgat catttgatta
      361 ctcggttaaa tacttttgac atgagtatca atgaaacatt cattaattat ggttgcttat
      421 ttcttaccgc ttttctggcc aatgctaatc cgccgattat tccggcatta tcacctaatt
      481 cgggcgtaat aatgtagcta tcaattggtg gtatttcaac atatccattt ataagctcaa
      541 caaatttatc ctgaactttt ttcttcatgt gttcttgttt cattacacca ccgccaaaaa
      601 taattctatc tggcgaaaac atcagtgttg tattgtatgc acattgggca aggtaaaagg
      661 cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg gccttttaca cccaaccgtt
      721 tttcaatcgc tggtcctgct gccatcccct ctaaacaatc ttggtggaag ggacagtttc
      781 ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg ccccatttcc ggatgactga
      841 acccttccac gaaagtccca ttgaacaagg caccacctcc gatacctgtt cccacagtga
      901 agtatgcaac acctgctaac cctttgccag aaccagcaac atattcacca tagcaagccg
      961 cattgacatc tgttgtccat tcaatcggaa tattgaaata ttgctttagc gttcctacaa
     1021 aattgaaatt ttgccaagca agttttggtg ttgatgtgat atgaccgaaa ctcgttgatt
     1081 tgcattgaat atcaattgga ccaaagcagc cgaccccgat gcctgccaat tgttccttaa
     1141 aaggtttaaa aaaatcaatg acctgcttca ttgtttcaac tggagttgtt gtgggaaaac
     1201 tgactctttc caaaatcgtt aattcctcag taccgactgc acagacaaat ttagtccctc
     1261 cagcttcaat tgccccatat atcatcatag gttcctccct tcgaatatgt gaaaaacact
     1321 tcattaacgc ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc ctgaagcttc
     1381 tgttgaatac cagtaagcga cactagcaac atcgtcttgc cgctcaaata agcgaacatc
     1441 atcgttgccg atttgttgta aagtgaccga taaatcttcg gtaaaacaga tggggtcttg
     1501 aagatgccaa cgataaagac catgtcgtgg cattgcgtcg tcaccaaagg catgaacggc
     1561 attcaatttt cccgtttcaa aacgatctct tgtggcatct ctatttgatt ggtaaggata
     1621 ccctaaaaag gctgttgaat agtttttcgc atgtggacgt ccgaactcat cgtactcatg
     1681 aaaggcccac gccccgccaa aataatcttc tgaacctgtt gaggttacgg tcggatactc
     1741 actatcccca tcaatgtaga atttaaactc gccttcgccc caccaataac gttctaggca
     1801 ggtaagcgca aggtaagtac ccacgtaata gccttgaccc tttaatcgat caattaactt
     1861 ataatcctcc tgtaatttgg taatgcgttc ccgatgccaa tttgcgtgaa aatataggct
     1921 attttcttcc aattcgtcca ccaaagcgta atttatcgta taaaaaaatg aggacatatc
     1981 cttcggatgt aaattctcga tcgtaatttt agcggatgtt tggaaaggca tttcgaaata
     2041 ggaattaaag ccccctgtag gattaaccac tatcggcaac gagttcacct cgcaacgctc
     2101 tccaaaccca ttacaaaaga aatcccctaa aggactttca acagcagggc tatccgaccc
     2161 atcccaataa attcttaaga ttaaatcacg taaaacaaaa ctgccaaatt ctgttcgatc
     2221 acgaatcgtc atccacatat gacgaattat tcccgttcca gtaatatccg caatcacagt
     2281 ttcttcacca gaagtcagcg gaattgcgcc acgacctttg cggcttggtc ctagctcgct
     2341 tggcaacatc gcagctttac ctttctcgcc tgttggattt tcgggagtga ttgctcgact
     2401 ctggattttt ttaaattggg taatattttt tagtatactc atctttttct cctttgaatg
     2461 tgaatcgttt ttcccttttc tacgtgaaga atttttttcg tatcacttat tttcaatggc
     2521 ttttctgaca aatttgaaat actcaactcg gattgcgtac attcaatcga aatgaaggct
     2581 tgaccgaact tcaaattctt ccaagaaaac gttaaattat caacaggatc aatttgcaat
     2641 actccttttt tgaatttcaa tccgaaatat tgactgatca ttaggctgac aaaaagaccc
     2701 gatgcccagc cgcatttgcc gcagccaaag tctcgcacga cttctccttg ttgagcctct
     2761 aagcgatacg gccaccacca ccacgaaccg tctaaatctg ccaaacaaat tagttcttct
     2821 aatcgggtta cgaaagcttg agggttgtgc agctcgctca ttaaaacagt gataaagcca
     2881 ggaaaagtgg cgccggattc taatccccaa gtgatgcctt tgatttcctc gctataggtt
     2941 ggattgaaag aactagcaga aaaggccatg gtgttctgat ataaatgatc tttattatct
     3001 aaaaattgat aaaaaggcat caacgtagtg tctgattctt ctccatcatg catcaataaa
     3061 ttaatttttc catccacaat cacatcactt aaaaagatta acccttgctc aagaatcggt
     3121 ttttgatagt gcttcactga ataggttgtt ttctcctcat caccgattcc ctccaaaaat
     3181 tgctcaccaa aaggaccatc gatagtcatt tgtttacaaa ttgattcctt tagtttacag
     3241 gcaatgtttt tgtatcgctt gctaagactc agttgttcta gggcttcaaa aatttctgca
     3301 agtccaatac aggccttcca aagacaaaga ttgctgccgg tatgatactt tccaagtgca
     3361 aaggcgtctg ataaccaaac agatggaaaa agcattggtt cccctgaccg acgttgagct
     3421 aatatgtcct caataattct tgaagcattc agtagaacct gcggacaccg ctcaaagaat
     3481 tttgtatccc cagtatgttc aaaataaaga ctagccagta acgcactgga taacgaatta
     3541 ctcagagaat gattgatacc tcctgtaaat tttgaaccag gaaacttaac gccatattga
     3601 tcaaaccata gaaccgtttt ttgacataac tccggttcaa acatggtgaa tggcaatgat
     3661 gcgtaataca tatccttatt ccaaattcga ttagtagcag gatagctgcc ccaattgctg
     3721 ccgacgacct gtccctgtcg attcataccg aaactagaaa agctttgata aagtgcacga
     3781 cgatagagat ggacaactct atcgtttggc attgacagct gcccaaatag ctgagcaaaa
     3841 tagttcaatg tttccttcag ccatttttcg atatcagatt cctgaaaagt cattacttct
     3901 tcaaaagcat ttgggtccac aaaagcaatg gcgaagcttg ccacttcctg ttgtgcgatc
     3961 gtgtccttat ttcctttttg agaaatcaac acattttgct gatccgaata cttctcttga
     4021 tacgaaggca catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa
     4081 acctgttgaa caagcatact taaacgctta ttagccagta tgggagcaaa ggatactaca
     4141 atgaccttaa acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg
     4201 acttcaatac gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt
     4261 aaggataacg aattggtttg agagagctgt ttattgaccc aaacacctgg tttttcctca
     4321 gttagtaaat tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt
     4381 gctaatgtct tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga
     4441 tagtagctgt attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaaaaat
     4501 gggtgtttgg gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa
     4561 actgcagcct agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc
     4621 ccaagacgac cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga
     4681 aacagctcaa tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt
     4741 ggacattcat tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca
     4801 tactgatcgt cattgcacct accatgatca gacccaagat actagcagct tttgtcagaa
     4861 tttcaattaa cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta
     4921 aaacgccgcc atagtatcgc atcaagtaat taggtatatt gaacataact aaaaagataa
     4981 ttggtgccaa aatatttccg gcattggcta agttgatcgc tagtcccgcg gtaacgacac
     5041 gccaaactcc ccagaacaat gaatcgccaa ttccagccaa tggtcccatc aaactcgttt
     5101 tgatcgcgtt gatactttcc acgtcaaaac cagctttttc agaattttcc ttctccatcg
     5161 atgccgcaat ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa tactgtgtat
     5221 gacgttccag cgccgcagct cgatcttctt cacttttgta gaaatgatta atgaagggca
     5281 tcatagaata ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa gcatacatag
     5341 tcatcgagcg ccaaaacatc ttccgaacca attttttctc atctttagaa attccatcag
     5401 ctaatttcat tgaaagaaat cctcctcttc ttggtcaagc gttgcattta cagcagcatc
     5461 gctttgaact gcatgaacag atatattttt tagttgtaga tctctttggg ctataacaat
     5521 tgcaataatc gttccaactg cggcaattgc aatcaatggt aactcgagat acgccataac
     5581 aacggcccca aagaaaaacc acacagcaat atttttactc caaagcatcc gtaacagcat
     5641 cgccatccca actgccggca gcagatttcc agcgactgtt aatccgttca tgatgacctc
     5701 tggaatatta tccaagacgc ttttgacaac atctgctcca aataaaacgg caaagaatgg
     5761 aataagagca taaagcgcat agtggacaaa ccacataaag aagtgcagat tgatgattcc
     5821 tttttgatca ccagattttg caaatttgaa aaatttagca gccatcggtc cacatacaaa
     5881 agaaaaaatc atattcatta actgtaatcc taatactgct agtggaaaga caatgggaat
     5941 aatcgcatcc acgtttcctc ccattttgat tgcgaacgct gtagccagcc cagcagccaa
     6001 agaaggctcc gccgtactag agattccgat attgacgaca cccataaaga tggcttctag
     6061 tgacgccccg acaatcaacc ccgtctgcaa atcccccatc aacgctcctg ctaacggagc
     6121 aacaacaatc ggacgactca gcatggacat cccgaacatt tcatgtccag caattccaat
     6181 aaaaacggcc aaagcgacta caaatgcatc atacataatt tttctccttc ctttttctaa
     6241 aaatcactca aaatagagat agctgaacgt ttttccatcg ccggtgtagg ctgaaaaacg
     6301 gtatctggat aaagttcaac taattctttt aagtcttgaa tttctgacgt cgttaacatc
     6361 acttcctttg ataaggtttt cttatcttcc gatggcggtg tcattttccc agcattcccc
     6421 acgttcatta attcgatttc cgttgggaaa gctttcgcaa cttttaccgc atctttgaca
     6481 ttagggacta aaaccatcaa gcgcatgtcc ttcgcccgcg gatcttttaa caaatgaatc
     6541 gcgtcatcaa ctgttttaat aatgactttg acattcgatg gtgcagccat cttcaatgtc
     6601 atcttttgaa tatcgttggt agccgcctca tcgcttgcta ctactaaccc ggttaaattt
     6661 agactctttg tccaagtcat cgctacttgt ccatgaatta atcgttcatc aattcgcact
     6721 gctttaatca tttttcttcc tcctaaaaaa aatcttcctc ttcatttact acatgtacct
     6781 catcaagaat ggtcatcttt aattcctgtt ggcatttatt taccagttcc tgaatatcct
     6841 cctttgttaa ttccttggtt gccaatacta tttcgagcag aattggcaga ttaaagccag
     6901 acaaaatagt tacagcaatc tctttctcca aaaaggtttt ggtaatcttc tgattgacac
     6961 ttccgccaaa taaatcggta aaaacaaaaa tctgatcctg ctcgttcacc tggtttacaa
     7021 atacattcaa ctcctgttca aaatcacact catccacata agcattaatc accgtaatct
     7081 cttgaacctt ccccgtcagt aattgaatac tgctaagaaa gcctgaagct aagtgaccgt
     7141 gagtcgctat tagaattttc ttcatatggt taattccttt ctttctgttc acttttatat
     7201 aagcaattcc tgtgccaact tttaaaaaca ttggtttaat cacgttaatt cacattaaaa
     7261 aaagtgtcta acctaaaaaa aacagattag acactttttt agacacttta tttttgaata
     7321 tacggtccta ataattcata gagcagcgaa agctcataag cattgacttt aaaattatat
     7381 ttcatctcca ttgattgaaa aatacttcta gaaacataat aaaattcatc ttcatctggt
     7441 ttcaaatcat aattcggttc ttcagatttt tctgtccgag ccaagaataa acgttccatc
     7501 atcaatgccg tatgcatata gagatttagt tttacacggc catttaaagt gaaatgatag
     7561 taattttcat atttagaaat gactgtttcc acttcactca tcacgatcgt tgggttcaga
     7621 aagttcaaac ggtccgcaat tccttccaac gaaaagaatt ttactagctc ctgaatcaag
     7681 cggttaaatt ctgccggctt catttcagga ctcaaaatat gctccaaatt ggctgctcca
     7741 tcaccgtcta agatgtcata aacattaata ttaggaattg aaaaggaacg tggaagatcc
     7801 gaagtcgtga tgaccaattt tgtcttgtca aaataagttt catcattttg gtcgattaat
     7861 tcacgtagtt ctctgtagtc cttcgtgaaa acttcgatgg attcatccga taaatgatga
     7921 cgaaaaacat ctttcaattt gtccgaaatc cccaaacccg acatacagga aatgataatg
     7981 ttgctacttt gagaaaagcc ttcaaagtat tgaacattga ttgaatactt gtctgctgca
     8041 tcctccgcaa tctgtttaaa tggcgcattc atcgtcatct tcaagccaat atcaagtgca
     8101 atggaagtcg tcaaattatt cagaagaagt aactcccctt ttaaatcgtt cttgatctgt
     8161 gtgtaaattt gatttaaaga gcccatatct acgatcagca ctagactatc tgtattcact
     8221 tgccgtttca cgaaattcct tgtcgattcg acaatctctg acacattact ctctactggc
     8281 atatccaacg cttcaaaaac ataattgccg cataattgat tcactactgc ttgaatgctg
     8341 ctagccgtac tatctccgtg agcaagcaac aggcccttca gctcgatgga ttcatctaca
     8401 taatcagata aagtcacgac aataagcagt cttagaaagt tctcgtcctt ttctgagagc
     8461 ggtggtaaat gctccaagaa ctgattacat aagtaagtcg tccgaggtag atttgcagct
     8521 aatgtacgat ttatcccggt taagtctgtc ttatagcttg gggaaatgac ataggatctg
     8581 ctaataattt ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc taaaacggat
     8641 acccagacct ttctaaacat ctgaatcatc aattttctac tcattgataa aaaagaatcc
     8701 ttatcaatgt gatgcaccaa atgctgtact tcattcttta tcgaagaaaa attttcattg
     8761 aaattacctt catttatcag ctgagtaatt ttttccaaaa tcgaatcata atcaaacggt
     8821 ttgattcgat ccgtttttat cttttgattt ttctttatca ataaactagc aatcggcaag
     8881 ctttctactt cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt
     8941 aatggttctt cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc
     9001 agcttaccaa tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa
     9061 acaataatat ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct
     9121 aatcgttctc gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc
     9181 cgatcaaagg tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga
     9241 accttagcag aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata
     9301 aatagaaaga gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga
     9361 tagccgttgt tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc
     9421 cccttcttgt atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca
     9481 cagtttaaga tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca
     9541 aaaatttttg tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg
     9601 agtccgttag gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt
     9661 ccgtcatgac caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc
     9721 gcatattcat ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta
     9781 tcttccacta atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc
     9841 gaaattttct cggcagtgac agaagacata ttccctgtta gtcgtatcag ctcatcaaaa
     9901 actcgatctt tagcgctctt tttcattttt tcctccagtt taatgtgtct aaaagcataa
     9961 taaactatcg gctgcaatat gtctattcta tatttgtatt ccaattttta ctttgaaaca
    10021 caaatagtcg aatatttgcc tcagatatct gacaaaataa tttatctacc aatttagttt
    10081 ttatctaata tgatttttat gttttttcat agctacaaat attgaactgg attcatatat
    10141 tagttttatt aatagattac agaagggtta agtatatttg catagtttcc aattacttta
    10201 tccttctgta actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat
    10261 ggttttgtcc acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa
    10321 aagcggcgtt tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat
    10381 ttttagctct ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct
    10441 atacatcttg aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc
    10501 tttgtagact tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga
    10561 aatgtctttt ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc
    10621 tgcattttgt gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc
    10681 tttatttttc acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg
    10741 tgggatcaca taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa
    10801 tgtcactgcc acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat
    10861 tgctttgaca tttggtgtta aatgattcaa tttatttgct gcttctgtca actgtgccat
    10921 attcttacta ttcaaagaat agcctaaact attcaaagaa agccccataa cttctcgtgc
    10981 accgtcaatc agcatcaaac tgtcttttaa ttccggcttc cacaaatcat cccaatgctt
    11041 aatttgattt gcatcaaaga atttatcgtt atatacgatc cctaaagttc cccaaaaata
    11101 tggaatcgaa tactgattat ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt
    11161 atttaatcct ttgatcttcg aatgatctaa tggaagtact agtttttctt tgatcatttt
    11221 ttggatcata tattcacttg gtatagcgat gtcgtagttt gtcccacctt gctggatttt
    11281 tgtgaacatc gcttcattag aatcaaatgt ttcataattc actttgtatc catattcttt
    11341 ttcaaatttc gtgatcaaac taggatcgat ataatctccc cagttataga tggtaagtac
    11401 ctttgctcca ctcatgccac ttgttttttc aagttgacga acaccaagca gcagcaccgc
    11461 aataatgatg agaataccta aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc
    11521 ccctcgcgta ctcttctgtt tttcttcagc cgttttggac ggttttcttt actaataaag
    11581 tagtatccaa tgaccaatat catggagaac aagaaaacta acgcacttag cgcattgatt
    11641 tctaaactga ttccttgtct tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca
    11701 tttcctgtga cgaaaaatgt aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa
    11761 tatccagcta tgattcctgg agaaagataa ggcaaaatga tatttttgac tacttggaaa
    11821 ttattagcac caaggtctct agccgcatcg accattgaat cgttcatttc ctgcaatttt
    11881 ggcaagacca tcaagacgac gattggaatg ctgaaagcaa tatgggaaag caatacactc
    11941 tgaaaaccta atccgaatcc tagtagtgta aagaaaatca agaaactagc accgataata
    12001 acatcaggag agactaataa gatgttattc atgctcaaca acgctgtccg tgcacgtcgt
    12061 tttttcgtgt aatagattcc catagcccca aatgtcccga tgatcgtggc gatcaatgca
    12121 gataagaaag ccagcatgaa cgtattcaat acgataatga tcaaacgtgt atcttcaaaa
    12181 acagcttcat aattttccca agtaaatcct gtaaagctgt tcatcgttcc tccttcattg
    12241 aaggaataaa aaatcagata gaagatcggt gcatataaga ataaaaagac gatccctaaa
    12301 taaagatacg accatttaaa ttttttcatt tatgagcgac ctcctttttt cttctcgcct
    12361 gtcaggagca tcacgatgaa catagcaatg attaaaatca caccgattgt agaacccatc
    12421 ccccagtttt gtgtaaccaa gaaatgctgt tcgatagccg ttcctaaagt gatcacccga
    12481 tttccgccaa tcagacgagt cagcatgaat aaagaaagag aaggaataaa tactgcttgg
    12541 acaccacttt tcacaccatt taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag
    12601 ctattcgcgc ctaaatctct actagcactg atcaaagaag gattcatctc ttccaatgca
    12661 ttgaaaattg gcataatcat aaatggaatc tcgatatacg ttgccacgaa cagaaaactg
    12721 aagtctgtga ataatatctg tttcggcccg atcccgataa aatcaaagaa atgattcacg
    12781 ctgccgtgca cactgaaaat cccaataaat gcgtaagctt taagcagcag attcacccat
    12841 gtaggcaaaa taatcagcat cagccataat tgcttgtgct tcagttttgt taaaaaataa
    12901 gcagtcgggt aactgatgat cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa
    12961 ttgatcgtca tagaaagcga tgtccccgac gtaaaataag atagataatt attcagcgtg
    13021 aattgcccat tcatatcaaa aaaagattga taaatgatca ttaatacagg cgcgatcacg
    13081 aataaaccga gccacaaaat ataagggacg gaatagatcc tgcgcattgt tttcattctt
    13141 gtccccccta ttcttcgtag ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga
    13201 aacgcatgac atggatatct tctggctcaa aggccaatcc tactttgctt ccttctttag
    13261 cttttttcgt tgaatggacc atccattcat tttgctgttc atcataacag atgatttcat
    13321 aatggacacc acgaaacagc tgagtatcga ccgttacgac tagttttcct ttatctggtg
    13381 tcgtgatcgt caaatcctct gggcgtaaaa caacttcaac tggttcattt agacgcatcc
    13441 cgccatcgac acattcaaat tgcttaccaa caaactcaac tagattatct tcgatcatta
    13501 caccgtccac gatattgctc tccccaacaa aatcagcaac gaaatgattg attggctcat
    13561 cataaatatc tacgggtgta ccactttgga cgattttccc tttattcatg acaaaaatct
    13621 catcactcat tgccaacgct tcttcttggt catgagtaac aaaaataaac gtgattccta
    13681 atcgttgttg cagatcccgc agttcatact gcatatctgt acgtagtttc aaatcaagcg
    13741 ccgataacgg ttcatctaat agcaatactt tcggttcatt tacaattgct cgtgcaatcg
    13801 ctacacgctg tctttgacca ccagacattt cactaatctc ccgtttttcg tatccaggaa
    13861 gctggaccat acgcaaagca tcttttacct tcttttcgat atctgatttg gacattttct
    13921 tgatcttcaa tccaaaagca acgttatcaa aaacattcat atgaggaaaa agtgcataat
    13981 cttggaagac tgtatttact tgtcgcttat tagctggcaa atcattcaac cgtttccctt
    14041 caaaatagat gtccccttct gtagcttcgg tgaatccagc tataatacgt aggatcgttg
    14101 ttttaccaca gccggaagga ccaagcagtg tataaaattt tccttgttcg atctcaaaac
    14161 ttactttttt taatactgtt tcatcatcat agcgtttcac tacttgatca aatgaaatga
    14221 ttggtttccc cacgttttta ccccctatat tacaaatatg attgcgttgc aacaatcaat
    14281 acttttgttc gttttttgag atgattcctt aattgatgtt gttttgtcgc ttgataatag
    14341 atcgactgtc ctgcttttgc cacatagacc gtctctccta agcttaactc taattcccct
    14401 tctaatacaa aaataaacgt ttcagataat gaaggttcaa acgtcttata ttctcctgca
    14461 ccttcaaaag tcagcaagac aggctccatt tctttttcat tcgaatcagc cacgagccat
    14521 tccaattcat acccgttttc ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc
    14581 acttgcagat tcgctgcttc ttgatgaaaa aattcttctg gagtcacacc taagacttca
    14641 agaatagaaa agaatgtttc cattgatggt gaacttaaat ctcgttccaa ctgggaaata
    14701 tacccttttg acaaatctgt gcgttcgcct agttcttctt gtgtcaaatt cttttggatg
    14761 cgcaaatttc ttaacttctc accaatttcc ataatttcct ccgaatcgaa gttttctaat
    14821 agtaaacttc ctgtttacta ccactcgtat tagtatacga aatttat
//
LOCUS       DAZKAS010000062        14815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000062 DAZKAS010000000
VERSION     DAZKAS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.064"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8H7_001603"
     CDS             complement(296..1024)
                     /locus_tag="ACG8H7_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983701.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8H7_001604"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8H7_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP8983702.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8H7_001605"
     CDS             2805..3743
                     /locus_tag="ACG8H7_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983703.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8H7_001606"
     CDS             3755..4666
                     /locus_tag="ACG8H7_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8983704.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8H7_001607"
     CDS             4690..6162
                     /locus_tag="ACG8H7_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP8983705.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG8H7_001608"
     CDS             6213..6824
                     /locus_tag="ACG8H7_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP8983706.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG8H7_001609"
     CDS             6854..8767
                     /locus_tag="ACG8H7_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFP8983707.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG8H7_001610"
     CDS             8895..11876
                     /locus_tag="ACG8H7_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFP8983708.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG8H7_001611"
     CDS             11873..13534
                     /locus_tag="ACG8H7_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFP8983709.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG8H7_001612"
     CDS             13574..14506
                     /locus_tag="ACG8H7_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFP8983710.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZKAS010000063        14678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000063 DAZKAS010000000
VERSION     DAZKAS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.065"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1087
                     /locus_tag="ACG8H7_001613"
     CDS             83..1087
                     /locus_tag="ACG8H7_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983711.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1088..2032
                     /locus_tag="ACG8H7_001614"
     CDS             1088..2032
                     /locus_tag="ACG8H7_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983712.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2032..2994
                     /gene="opp4B"
                     /locus_tag="ACG8H7_001615"
     CDS             2032..2994
                     /gene="opp4B"
                     /locus_tag="ACG8H7_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFP8983713.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3021..3935
                     /locus_tag="ACG8H7_001616"
     CDS             3021..3935
                     /locus_tag="ACG8H7_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983714.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3961..5742
                     /locus_tag="ACG8H7_001617"
     CDS             3961..5742
                     /locus_tag="ACG8H7_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8983715.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6090..6776
                     /gene="rnc"
                     /locus_tag="ACG8H7_001618"
     CDS             6090..6776
                     /gene="rnc"
                     /locus_tag="ACG8H7_001618"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP8983716.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6796..10377
                     /gene="smc"
                     /locus_tag="ACG8H7_001619"
     CDS             6796..10377
                     /gene="smc"
                     /locus_tag="ACG8H7_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFP8983717.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10386..11195
                     /locus_tag="ACG8H7_001620"
     CDS             10386..11195
                     /locus_tag="ACG8H7_001620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8983718.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11206..12204
                     /gene="ftsY"
                     /locus_tag="ACG8H7_001621"
     CDS             11206..12204
                     /gene="ftsY"
                     /locus_tag="ACG8H7_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP8983719.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12391..14658
                     /gene="gshAB"
                     /locus_tag="ACG8H7_001622"
     CDS             12391..14658
                     /gene="gshAB"
                     /locus_tag="ACG8H7_001622"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFP8983720.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT         5023 a         2639 c         3223 g         3793 t
ORIGIN      
        1 ttgttataaa acgatttatt ctgtgtaatg tcctttttta ttaagaaaaa cttccattta
       61 ggaagcagag aggggtattc gattgtccga taaccaatta ttagaagttc agaacctaca
      121 tacaggattc cgactcaaag atgactatta cgatgcagta gatgacgttt catttacttt
      181 agacaaaaac gagattttag caatcgttgg ggaatctggg tgcgggaaaa gtaccttggc
      241 tactacgatt atgggattac ttgatcctaa taacacaaaa attacaggtg agattttata
      301 taacgatctg aatttaatcg acctgaacga aacgctctat aataaaatcc gcggaaatga
      361 tatcggaatg attttccaag atccgttgtc tgcattaaat cctctcatga gaatcgaaga
      421 ccagatcaaa gaaggacttt cctatcacac aaagatgaat gcagaacagc gtcaagctcg
      481 tgcactagaa ttgctagaac aagtagggat tcctaatcct gcgagagttg gacgccaata
      541 tccccatgaa ctatcaggcg gtatgagaca acgtgtcatc atcgcaatcg caatcgcctg
      601 caaacctcca atcgtcattg cagatgaacc gacgacagcc ctagatgtaa ccatccaagc
      661 acagattctt gatttactga aagaccttca ggaagaaaca aaaacaggga tcatcttgat
      721 cactcatgac ttaggagtcg tagcagaaat ggctgatcga gtggcagtga tgtatggcgg
      781 acagttcgtt gaagtagcag gtgtaaaaga actgtttgat catcctaagc atccttatac
      841 acgctcttta ctgaactcta taccacaaga aggaaatcat gatgcagaac tccatgtgat
      901 cgaaggtgtc gttccttctc tgaaaaacat gcctcgtaaa ggttgtcggt ttgcaccacg
      961 tattccatgg ataccagcaa gcgatcatga agaagagcct atccttcatg aagtcgcacc
     1021 gaaccacttg gtacgttgct cctgctataa gcatttccac tttagagacg aaaaaggaga
     1081 ggtgtagatg acagaattaa tcacaatcaa agacctgaaa gttcattacc ctattcgcag
     1141 tggattcttc aatcgagtaa cggatcatgt atatgctgta gatggtgtgg attttattat
     1201 cgaaaaagga aagacatatg gtttagtggg agagtctggc tctggaaaat ctactacggg
     1261 aaaagccgtt gtgggattag aaaaggtaac agccggacaa atcatgtatg aaggaaaaga
     1321 tgtaacgaag cgaagcaacc gcaaaaaaat gggttataac aaggatgtgc agatgatctt
     1381 ccaagattca atgtccagtt tgaatcctaa aaagcgagtg ttggatatca ttgctgaacc
     1441 catccgaaat tttgaacggt taagcgatca agaagaaaag aaaaaggtaa aaggactgct
     1501 agatatcgtt gggatgccgg aagatgcgct atataaatat cctcatgaat tttcaggcgg
     1561 acaacgtcaa cgtctaggag tagcccgtgc tgttgctacc aatccaaaac tgatcgtagc
     1621 cgatgaacct gtatctgctc tggatctatc tgttcaagcg caagtattga atttcatgaa
     1681 acgaatccag caagaatacg gcctcagcta tttattcatt tcacatgatt taggtgtagt
     1741 aaaacacatg tgcgacaata tcgcaattat gtacaaaggt cgatttgtag aaatcggtac
     1801 aagagaagac atttataatg acccgcgaca tatctatacg aaacgtctgc tttcagctat
     1861 tccgcaaatc gatgtagaaa atagggaaag ccacaaagaa aatcgacgaa gagtagaaca
     1921 agagtatatc caaaaccaaa aagaatacta tgatgcgact ggtcgagtct acgatttgcg
     1981 tacgatcaca actacacaca aagtggcgtt gaaagatgga ggtgcaagct aatgtggaaa
     2041 acgattctgc gacgtgtttt attgatgatc ccgcaagtta ttattttaag tgttctgatc
     2101 ttcttattag cccaagcgat gccgggggat ccatttaccg gtttgatcaa tccaaatcag
     2161 gatccggcag tgatcgaaca gatgcgacaa gcagctggct tgaatgaccc atggtatgtc
     2221 cagtacttcc gttggatcgg aaatgctttg caaggcgatt ttggtcaaag tttcttatac
     2281 aaacgctctg ttgctgactt gatcggcgaa cggatcatca acactttata tctatcgatc
     2341 ttgacagttg tccttactta tttgattgct gtgcctctgg ggatgcttgc tggccggtat
     2401 caaaattcaa tcttggataa gattgtcgta atctacaact tctttagttt tgctgttcct
     2461 ttatttatct tcggcttgat catgctattt gtttttggtt atcgtttagg ctggttccca
     2521 acaagtggat cacaaacact aggtttgcaa ggtggattta tcactcaatg gatagaccgt
     2581 ttgaaatata ttctgttacc atcaattacc caagcatttt tggcgactgc cgtcacaatc
     2641 caatatctgc gaagtgaagt aatcgattcg aaatcgctcg attttgtcag aacagcccgt
     2701 tccaaagggg taccgacgaa taaagtattc agtcgacata ttttccgaaa tgcagcgttg
     2761 ccgatggctt ctcaaatggg gtatgaaatc acttcgttga ttgctggttc tgtagttatc
     2821 gaaaagattt ttggttatcc gggagtcgga aaactattta ttgattccat cggccaacgg
     2881 gactataccg tgatcacatc cttagtattg attttaggaa tcgcgacgct agtggggaca
     2941 ttactttcag atatcatcat gagtatcgtt gacccgcgta ttcggattca ataaaaccaa
     3001 tcagaaagga gaatggatcg atgagtgaaa aaaacaatac agcagttcaa gaaagtattc
     3061 ctcctatggg gatacgaatg attgctcgag aatttaaaaa agataaaatt gctatttttt
     3121 cattaggttt gctagtggtt ttattattag tgatctttat cggtgcgatg ctgaccgatc
     3181 aagacaaagt gatgtatgtt agtatcttcg acaaatacgc tgcaccagga acagtttcga
     3241 cacaaggaca aaagttcatt ctgggagcag atgaaggcgg acgtgatgta ttaggacaat
     3301 tgatcatagg tgctagaaac tctgttacga tcggatttgc gatcacgatc cttacttcga
     3361 tcatcggggt tggattaggg atcatctctg gctactacgg agggatcatt gacaacatct
     3421 tgatgcggat cgttgatttt atcatgattc taccgatcat gctgatcatt atcgtcttcg
     3481 tatcgattat ttccagatac aatgtctggt cctttatatt catcatgagt gcattttatt
     3541 gggtagctaa agcccgatta ttccgaagca agacattatc tgaagcaaga agagattatg
     3601 tcagtgcctc aaaaacaatg ggaaccaatg attttaaaat catgttccgc gaaatcatgc
     3661 cgaatctaag ttcattaatc attactaatt tgacgatgaa ctttgcggca aacatcggta
     3721 tcgagacaac tttgactttc ttaggtttcg gcttgccggc aaatgtacct agtttaggta
     3781 cattgatcgg ttatgcaagt aacggagaag tcttagtgaa tcggcaatgg atctggttac
     3841 ccgcatcaat ccttatttta gttttgatgt tgagtatcaa ttatgttgga caagcattca
     3901 agcgttctgc agatgcgcgc caacggttag gctagtatag aagcgaaggg ggaaatagga
     3961 atgaagaaaa cgatttttgg atttgcagcg ctgatgtctg ctgtcgttct tgcagcatgc
     4021 gggggcggag gtacagatac ctctggaaac aactctggag gcaacggcaa taacaaagag
     4081 gaattaactt ttccaattgc aacaaaaaat aatgatgatg caatcgaaga cggcgaatta
     4141 gaagctgcgg tcgcaacgga cagtcagttt aaaggattgt tccagtggga gttttaccaa
     4201 gattcatatg atgcagcttt tatggctcct tctcatgaac cgctatttgc aaacgatgac
     4261 gatttcagaa tcacagacga cggagctgca acgcttgatc tagatgaaga tacgaaaaaa
     4321 gcgacaatca aagtaaaaga caacgtcaaa tggtcagatg gtgaagatgt aacggcggac
     4381 gatgtgattt tcccttacga aatcattggg aataaagatt acactggtat ccgttatgac
     4441 gacactttcc gtaacatcgt aggtatggaa gaatatcatg atggaagtgc cgacacaatc
     4501 tccggaatca aaaaaataga cgacaaaaca gttgaaatcg aatataaaga agtaaaccca
     4561 agcatgcttc aatcgggtgg tggtatctgg tcatatgcag caccaaaaca tacgttagaa
     4621 ggtgtagcaa tcaaagatat ggaatcaagc gatcaagtac gtaaaaatcc agtgactttt
     4681 ggcccttatt acatgagtaa tatcgttgca ggagaatcag tagaatactt gccaaacgag
     4741 cactactgga atggtacgcc taaattgaaa aagatcacga tgaaatctgt accgatggca
     4801 agtgcaactg aagcattgaa ctctaaatta tatgatatga ttttccaaat gccaacagat
     4861 acttacgata cctataaaga aattgctggc tatacaaact taggccgtga acaaacttct
     4921 tatacttatc taggattcaa gttaggtaaa tggaatgcag atacaaagag tgtggaatac
     4981 aatccagatt caaaaatggc tgataaatca ttacgtcaag caatgggcta tgcattagat
     5041 aataatgcag taggtgaacg tttctatgca ggtttgagaa gcaatgcgac ttcattgatt
     5101 cctccagtat tccagtcata ccatgatgat gagatgaaag gattcacaca agatctagac
     5161 aaagcgaaaa aactattaga tgatgctggc tacaaagatg tggatgacga tggtttaaga
     5221 gaagacaaag atggaaataa actaacaatc aactttgctt cgatgtccgg tggggaaaca
     5281 gcgcaaccat tggcagatta ctatatccaa caatggaaag aaattggttt ggatgtccaa
     5341 tacacaacag gtcgtctgat cgaattcaat tctttctatg ataaattaga aaacgatgat
     5401 cctgaaattg atgtttacca agctgcttgg aatacaggaa ctgatccaaa tccaagtgga
     5461 ctatggggac caaatgctgc gttcaactac actcgttatg aatcagaaga aaatacaaaa
     5521 ttgatcaacg atatcagttc aagcaaagca tttgatgaag agtaccaaaa agaagcatat
     5581 caaaaatggc aacaatatgc atttgaagaa gcatttgcga ttccaacgtt gttccgtaat
     5641 gaagtattgc cagtcaacga ccgtgtcaaa gactgggatt ggtcttatga tgtaccaaac
     5701 ccatgggcaa atgtttctgt tacagcagat tcacgttcat aatcactaaa aatgaccgaa
     5761 aaagggcgat gtcctttttc ggttttttga tatttttctg tttatttctc taatattcag
     5821 gtcaaggcgc tgatttcatg acttctccaa taaacggtgt tcaaagtcca gtttctcggt
     5881 attaagtcaa atttatgcaa aaatatggaa agccattttc acaaaatttt ttcttcatac
     5941 tcgaaactaa acgaattttt cacaacttct cgtcaatcac taggattatt aaagctttac
     6001 ttaccttttg accgaaccat ttcaaatttg acaaaaaacc agtataataa ggaagtatcg
     6061 aaaaaatatc cataaaagga atgtggaaaa tggacaacca gctgacaaaa gaactaaaag
     6121 aaaactataa catcgttttt cacgatctaa atttgcttga acaagctttt acccattcat
     6181 cctatgtgaa tgagcatcgc aatctgcaat tatcggataa tgagcgactt gaatttttag
     6241 gagatgccgt tttagaactg atggtttctc agtatttatt ccgtttatat cctcatttgc
     6301 cagaaggaaa attgacaaaa acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat
     6361 ttgccaaaga atgtcacttt gatcaatatg ttttgttggg caagggagaa gagaattcag
     6421 gaggacgtac gcgaccagca ttgctttgcg acctattcga agctttttta ggcgcgttat
     6481 atcttgacca gggattcgac acgacccatg cattcatcga aaaagtgatt tttcctaaag
     6541 tcaaagcagg tgctttttca catgagatgg atcataaaac aaaactacaa gaagtcttgc
     6601 aaaaatcagg tgatgtatca atcgaatacc gattgatcaa tgaagaaggt ccagctcatg
     6661 aacgagtatt ttggatcgaa gtttatgtag atgatcaatt gattggcact ggacaaggga
     6721 aatcgaaaaa attagcagaa caagctgcgg cggaaaatgc attggcagca ctgtaaaagt
     6781 aagtgaggga aagctttgta tttgaaacga attgaaatag ccggttttaa atcatttgca
     6841 gacaagacag tcattgattt tgaaaatagc gtcacagcag tggttggacc aaatggcagc
     6901 ggaaaaagca atattacaga ggctatccgc tgggtattag gagaacaatc tgctaaaagt
     6961 ctgcgaggcg gaaaaatgcc ggatatcatc tttgccggtt ccgatacccg taagcagctg
     7021 aatattgcag aagtcacagt gattttagac aatagtgatc attatctgcc attggagtac
     7081 aatgagatca gtgtaacccg ccgttatcga cggactgggg aaagcgagtt tttcatcaat
     7141 aaacaaagct gcagattaaa agatatccaa gaattgttct tagattctgg attaggaaaa
     7201 gaatcgttct caatcatttc tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa
     7261 gaccgtcgtg ggatctttga agaagcagct ggtgtcttga aatacaagca gcggaaaaag
     7321 aaagcagaac agaagctttt tgaaacagaa gacaacctca gccgcgtcca agacatcatc
     7381 cacgaattgg aagaacagtt aactcctttg gccgcccaga gtgaggcagc aaaggagttt
     7441 ttacggctaa aagaaacact gactcaaacc gatgtctctt tgatggttgc tgaaatcaag
     7501 acagcaaaaa aagattggga caacaaacaa gcacagcttg cgaaattcaa tctagaatta
     7561 ggcaaactat ctgagtcgat ccaagaacaa gaatccattt tggcgaagca gcgtaaagaa
     7621 aatgcgcaag ccgatcgtct gatcgaaaaa aatcagcaag tattattaga tctttcggaa
     7681 aaactgaagc agacagaagg acaaaaagat gttttgcagg agcggacaaa gcatacacaa
     7741 aaaagctcgc aagaatatca aacttcatta gcagaagcac aaaaaaaggt taaacatttt
     7801 gaaaagcttc aagaaagctt gatgaaagca gcagcagaaa aagaaacaga gatccaaaaa
     7861 gctgaagcaa atctcataaa aacacagcaa gaattagaaa aataccaaaa atcaacgaaa
     7921 gaattattgg cagaattaag agatcaatat gttgatttga tgcaagaaca agcagctgta
     7981 ggaaacgaac tgaagtatct agaacgtcaa tatatacaag aaacagcaaa aagcaagcag
     8041 acgttagcca agcaatcgga agtagaagca tcagtagacc gattgatctt acaaaaagaa
     8101 gaattgacac aaaagcaggc gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta
     8161 gaaatgatcc aacaaaacgg gaaaaagttc caagaagcat tagcaaaaga acagcctaaa
     8221 atgtatcaat taatgaatca agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa
     8281 atccaagaaa actattttgg tttttaccaa ggggttcgat tggtcttgca gcataaacag
     8341 cagctttcgg gtattgtcgg agcagtggct gaactgatcg atgttcctgc agatttcacg
     8401 ttagctatcg aaacagcttt aggtggggca gcccagcatg tcatcgttga aaatgaaaaa
     8461 gatgctcggc aagccatcac gtatttgaaa cagcaaagag gaggaagagc aacattcctt
     8521 ccactgacga caatcaaacc aagacaatta ccggctcata tattgacaca agcagcagct
     8581 gtagaagggt ttatagggat tgccagtgaa caagtcagtt atcccgatca aatccaaact
     8641 gtggttcaaa acctgctagg aacgatcctt ttagccaaag acttaactag tgccaatgca
     8701 atcgcgcaaa cgatccgcta tcaatatcga gtggtttcct tagaaggtga tgtgatgaat
     8761 gccggaggat ccatgacagg tggagcgaat aaacgaggaa accaaggcag tttgtttgta
     8821 caaaatcaag aattgaaaca gttgactagt gaatttgaag aagcagataa acaactccaa
     8881 gcacaagaaa aaaaagttca ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa
     8941 gaagttttgc gtacacgagg cgaacaatta cgttttgaag aacaagaagc gacaaatcag
     9001 ctacaaaaca tcataaatga acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt
     9061 gagacacgtg aactgcagca gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa
     9121 gacaaacaaa cagatcttga atcgcaacga caaaaaatcg acgaagaaat caaatcgtta
     9181 agtcaagaaa gcgaccagat ggaagccaga agagcacaag tacaaagtca aaaagcacaa
     9241 gaacaagcag atcttgctgt cttgaaagag cagtttaatc atctgcagat ccagctccgc
     9301 ggtgcccgag tccaaaaagc agaggcaacc gaacgtcaag aagcgataga aaagcagttg
     9361 gcgacattga cagcagactt ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa
     9421 attaacgaat tatctgctca gcgagaaaca ttaaaagccg agttggcaaa agccaaagag
     9481 cagcgagata gaaggcaaaa ggagatcgat aagcttgaag cagtcttggc agagcgtaat
     9541 cgtgagcaaa aagcacgact gtcggaacaa tccaaattag aagtccaaaa agaccgagct
     9601 gaaatgattt tagataatca tttgacctat ctgcagtcag aatatcagct gacatttgag
     9661 aaagcttccc aagactatca agaaacaacc gatatcgaag acagtagaac aaaggtttca
     9721 tccttaaaag aacagattga aaaactggga ccagtaaact taaattcaat cgaacaatac
     9781 gaacaagtca gtgaaagaca tacattctta gcaacacagc gagatgatct gttagcagcg
     9841 aaaaaccaat tgtttgaaac gatggatgag atggatgatg aagtgcggac aagattcaaa
     9901 gaagtatttg aagctatccg tcaagaattc aaaattgtct ttccaaatat gtttggcggc
     9961 ggtaaagctg aattagtact gacggaacca actgatttgc tgaaaacagg tattgagatt
    10021 gaagtacaac ctcctggcaa aaaactgcaa agtctaagtt tgctttctgg aggagaacga
    10081 gcacttacag ccatcgcgtt gctcttttcc attatccgtg tctgtcctgt tccgttttgt
    10141 atcttagatg aggtagaagc agcgttagat gaagccaacg tgaaacgttt tggtcgttac
    10201 ttgagcgatt tccaagatga cacacaattt atcgtagtca cacaccgcaa aggaacgatg
    10261 gtcgcagcag atgtactgta cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt
    10321 tctgtacgga tggaagatat caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa
    10381 cgaaaatgat caaattagta gcaatcgatt tggacggtac attattaaat gaacaaaagc
    10441 tgatttcaga tgagaataaa caagcattag cacaagcaaa acagcaaggg gtcaagatcg
    10501 ttctatgtac aggacgcccc ttagctgcga tggcgcatta tctgcaagaa ttaggactgg
    10561 tagatgaagg tgactacagc atcactttca acggcggttt ggtacaaaaa aatgatactg
    10621 gtgaaatcat ggaaaagaaa gtgatggaag tatcagatat ccaacgctta tacaaactgg
    10681 ctcagcaatt ggatctgcca ctagatgttt tgtctgacaa tgtggtttta cagctgccta
    10741 gtgccccaag gaagcaatcc ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag
    10801 cggatataag cgatattaca gatgaatttg tgcttaataa ggcagtaatc gcttatgacc
    10861 aagaagaatt agatcctaaa atcaaagaaa taccagcaga attccatgaa atgtatgaga
    10921 tcatcaaaac aagaagtatg cttttggaat ttatgccaaa aggtgttaca aaagcttatg
    10981 gtatttcttt attggcaaaa gatcttggtt tgaaacaaga agaaatcatg gcgatcggtg
    11041 atgaggaaaa tgatttaccg atgatccaat atgctggact aggcgtagcg atggagaatg
    11101 ctgtgccttt cgtcaaagaa gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg
    11161 tagccaaagt gatccaaaaa tttgtattag agtaggggga gtcaaatggg attttttgat
    11221 aaaatcaagc gtgcttttac tggcgaagag aaaacagaag aagttgaagt acaggagaag
    11281 tacgacaagg gtttagaaaa gacgcgtaaa acatttggtg aacggatgaa tgagttgttt
    11341 gccaattttc gaacagtcga tgaagacttt tttgaagaat tagaagaaac acttatcggt
    11401 gcagatgtag gttttgaaac agcgatacga attaccgaag cattacgtca agaagtgaaa
    11461 ctcagaaatg cgaaaaaatc ggcagaagtc caaaatgcga tcatcgagaa acttgtggat
    11521 ctctatgaag cggaaggaat caatgaagta aatgaactaa atatccaaga agacggacta
    11581 acagttattt tgtttgtcgg tgttaatggt gtagggaaaa caacaagtat tggtaaactt
    11641 gcccatcagt tcaaaaacga aggtaaaaaa gtattgatgg cagcagcaga taccttccgc
    11701 gctggagcaa tcgaccagtt agttgtctgg ggcgaacgcg ctggtgttga agttgttcgt
    11761 ggcaatgcag gaggcgatcc agcagctgtc gtattcgatg ctttggaaag agcaaagaca
    11821 gagcaggcag atgttttact agttgatact gcaggccgac tgcaaaataa agtgaacttg
    11881 atgaacgaat tagaaaaaat aaaacgagta atccagcggg aattaccaga agcaccccaa
    11941 gaaacacttc ttgtattaga tgcaaccact ggtcaaaacg cgatggttca agcaaaacaa
    12001 ttcaaagaaa caacagatgt gaccggtctt gttttgacaa aattagatgg tacagcaaag
    12061 ggcggtatcg tacttgctat ccgtaatgaa ttgcatttac cagtgaaatt ggtcggtctt
    12121 ggtgaaggaa tcgatgactt ggagccgttt gatccaaatg attttgtcgt tggattgttc
    12181 aaaggattac taaaagaaga ataacccatg tctaaaaagc aggagctgtg acaaaagttg
    12241 cagctccttg tttgaatgat tgtttttaac atagctgtgt ttttgatgcc ttcattttta
    12301 tacttatttg taagcggttc tcaaacaatt tcgttctttt tcttagactt tttgattata
    12361 attagattca tatgaattgg aggtcactta atgatgaatt ttaagcaatt attattgcat
    12421 gtcaatgcgc gcccttttat cgaccaagct cgatttggta tagaacggga aggacagcgg
    12481 gttgatcttg caggaaatct agcaaaaacc gatcatccag caatctttgg cgatcgatcc
    12541 tatcacccct atatccaaac agattttagc gaaacacaaa cagagatgat cacccctgtt
    12601 accgattcta ttcccgaatt atttcagtat ctcgctgctg tttatgatgt gactgctcgt
    12661 tctataccga aagaagagat gatctggcca ttaagtatgc cacctgcctt accggaaaaa
    12721 gacgaagaga tcattattgc aaaattaaaa aatttcgaag atgtcttgta tcgacgttat
    12781 ttagcaaaag aatacgggaa gcgaaaacag atggtaagcg gtattcattt caattttgaa
    12841 tttggcgatg agttgctaag aacattgttc agccatcagg aagaatttca agatttttcc
    12901 gaattcaaaa cagaacttta tttgaaaaca gcgagaaact ttatgcgcta tcgctggatg
    12961 atcacttatt tattcggtgc ttcaccaatg agtgagaaga actacttttt agatgaatca
    13021 catccgcaag aacctgttcg cagtattcgc aacagtgcgc tgggctatac gaatcatcca
    13081 aatgtgaaag tttcctatgc ttctatgaaa cagtatttag cagatatcga gcgaatgatc
    13141 gaagaaggga aactttcgga ggagaaagaa ttttatacac cgcttcgttt ccgaggcgga
    13201 aagaaagtcg cagatttagc aacaacaggt gttcgttata ttgaattgcg gaatatcgat
    13261 ttgaatcctt atgcaagatt gggtatcaat ccagagcaag ttcggttctt acaattattc
    13321 ctgatgtaca tgttatggac agaagaaaaa gaagactgtg accaatgggt agcagaaggt
    13381 acgactcgaa ataacaaagt agcactagaa caaccatcag atcaaacaga atttcatcaa
    13441 gaaggtagag aaattcttga aggaatgaag caaatgctgg ttgaattgga ttggttggat
    13501 tctctttatt tagtagagga agcattgact caaatggatc atcctgaaca aacgttagca
    13561 gcgaaactct atcaagaagc acagctatcg agtcagcaag aagttgctgt ggcattggga
    13621 catcaatatt ataaagaaag ccatgaacgt ccctatcagt tggccggttt tcgcgaaatg
    13681 gaactttcta ctcagatatt tatgttcgat gccatccaaa aaggtgtcca agtcaaagta
    13741 ttagatgagt cggatcaatt tttgcgtctg caattccaag accatgtaga atatgtgaaa
    13801 aatgcaaaca tgacaagtaa ggacagctat atcgtcccac ttatcatgga aaacaaaaca
    13861 gtaacgaaaa aagtcttaaa agaagctggg ttccgggtac caggaggcgc agaattttca
    13921 tccatggaag aagcggtaaa agcttatccg agatttgccg agcaggcatt tgtgatcaaa
    13981 cctaaatcaa ccaattacgg gttaggtatc acgattttca aggaaggagc tagtctggaa
    14041 gattatcaag caggtttagc cattgctttt cgtgaagaca gttcggtttt agtagaagaa
    14101 tttatgccgg gaacggaata tcgtttcttt gtgatcgacg gagaagtcca agccatcatg
    14161 ttgcgagtcc ctgctaatgt tattggtgac agtatccgaa cagtgaaaga acttgttgaa
    14221 gaaaaaaata gtgacccttt gcgtggaacg aatcatcgtg cacctttaga attgatccaa
    14281 ttaggtgaat tagaacaatt gatgttgaaa gaacaagggt tgacgatcga atcagttccc
    14341 caagccaatc aaattgttta tctaagggag aactcaaata tcagtacagg tggtgattcg
    14401 atcgatatga ctgatgaatt ttctgaagct tacaaaaaaa tcgccgtttc tgcggtagaa
    14461 gcactaggag ctaagattag tggtatcgat ttgattatac cagataaaga aatcgatcct
    14521 acaactgata aaaaagcata tgggatcatc gaagcaaatt tcaatccagc catgcatatg
    14581 catgtttatc cttttgcagg aaaaggcaga agattgacga tgaatgtatt gaaattgtta
    14641 tatcctgaag tattttaaaa gaaaaagctt ttgacaga
//
LOCUS       DAZKAS010000064        14672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000064 DAZKAS010000000
VERSION     DAZKAS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.066"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1052
                     /locus_tag="ACG8H7_001623"
     CDS             156..1052
                     /locus_tag="ACG8H7_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP8983721.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1202..2143)
                     /locus_tag="ACG8H7_001624"
     CDS             complement(1202..2143)
                     /locus_tag="ACG8H7_001624"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP8983722.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2269..3390
                     /locus_tag="ACG8H7_001625"
     CDS             2269..3390
                     /locus_tag="ACG8H7_001625"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP8983723.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3648..5150
                     /gene="eat(A)"
                     /locus_tag="ACG8H7_001626"
     CDS             3648..5150
                     /gene="eat(A)"
                     /locus_tag="ACG8H7_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP8983724.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5217..5744
                     /locus_tag="ACG8H7_001627"
     CDS             5217..5744
                     /locus_tag="ACG8H7_001627"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP8983725.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5955..7016
                     /locus_tag="ACG8H7_001628"
     CDS             5955..7016
                     /locus_tag="ACG8H7_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP8983726.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7019..7915
                     /gene="murQ"
                     /locus_tag="ACG8H7_001629"
     CDS             7019..7915
                     /gene="murQ"
                     /locus_tag="ACG8H7_001629"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP8983727.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7929..9911
                     /locus_tag="ACG8H7_001630"
     CDS             7929..9911
                     /locus_tag="ACG8H7_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP8983728.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9976..10422)
                     /gene="nrdI"
                     /locus_tag="ACG8H7_001631"
     CDS             complement(9976..10422)
                     /gene="nrdI"
                     /locus_tag="ACG8H7_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP8983729.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10633..12057
                     /locus_tag="ACG8H7_001632"
     CDS             10633..12057
                     /locus_tag="ACG8H7_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP8983730.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12238..13011)
                     /locus_tag="ACG8H7_001633"
     CDS             complement(12238..13011)
                     /locus_tag="ACG8H7_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP8983731.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13008..13913)
                     /locus_tag="ACG8H7_001634"
     CDS             complement(13008..13913)
                     /locus_tag="ACG8H7_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983732.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14067..14387)
                     /locus_tag="ACG8H7_001635"
     CDS             complement(14067..14387)
                     /locus_tag="ACG8H7_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP8983733.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4689 a         2613 c         3103 g         4267 t
ORIGIN      
        1 cttttaggcg aattttaata gaagaatgat ttatttcatc atattttcat acaaatgtta
       61 aattttcata caaatatagg aaacgtttta ctggcttttt actatttaaa caggctatca
      121 taggaaaaag tgacagagaa agtgaggcgg aaaagatgaa caaaaatgac gtactgctag
      181 ttgcaaaaaa taaatcactt tattttttgc cttccatccg ctctttagat gtttggtccg
      241 gaaaagattt actacagtcg gaattcatta aatcagctag acaacagttg gcaaattatc
      301 agctgatcgt ttttttagat tatggatttg atatggagat ggctcgacgc gtgcgcccat
      361 ttaccaatgc aaaaattgtg ttattttttt ggaatcactt caaagcagaa cacgaggcga
      421 tgctgaagca ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg
      481 aagcaaaaga acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt
      541 cttctagcga accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac
      601 gagcagaaag ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc
      661 ttccacatcg agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa
      721 caggggaatc gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc
      781 ggacatttga atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc
      841 attatcattt ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat
      901 taccggaatt tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg
      961 atgcagaaaa ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg
     1021 aatatgacct ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta
     1081 ctgaaacaaa caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt
     1141 ttattgggct taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct
     1201 tttattctgc gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat
     1261 atggtgtagc aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga
     1321 tttcagtcaa acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga
     1381 tgttgtcagg atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga
     1441 ttccttcttt tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg
     1501 ccgctttttg cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca
     1561 atgttccatt tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg
     1621 gatttaaatt tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg
     1681 gttccaagtt ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag
     1741 ctagaccagc tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt
     1801 ccagtacatc ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa
     1861 taatggattg gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga
     1921 tcaagtccac gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg
     1981 cgaccacttc tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt
     2041 gagcaggcca acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga
     2101 ttcggctgcc cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta
     2161 ttcaatgagt tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga
     2221 aaaaaatgaa aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg
     2281 gcggcaattg attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta
     2341 aatggctggg aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa
     2401 aatactgcag aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg
     2461 gatctcttat tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac
     2521 accgcagtca tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat
     2581 caaactatcc gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt
     2641 caagaagtag aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta
     2701 ggattactcc aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta
     2761 tctactaaaa aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt
     2821 atcgctgcag atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga
     2881 agacaaaaag gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt
     2941 gctcagcaaa taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca
     3001 gcaggcggta ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat
     3061 ttagtcagcc aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca
     3121 ggagaagggc ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg
     3181 cttgcaaaaa aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta
     3241 gaaggacttt ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta
     3301 gaagaagcaa tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt
     3361 agcagatttt tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt
     3421 aatttcaaat ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga
     3481 aagctgcatt cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt
     3541 ggcttggttt tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta
     3601 gccagttctg aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg
     3661 aaataaaaaa tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa
     3721 atctaaattt tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga
     3781 caactttact gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc
     3841 aagaatttgc ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt
     3901 taaatgatat tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa
     3961 cagatcctga aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag
     4021 tcctactggc acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga
     4081 atcatttgga tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag
     4141 gcttcatcgt ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag
     4201 caatcgaaaa aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga
     4261 aaaaacttcg tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta
     4321 ggctaaagaa aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa
     4381 caaagaaaca agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg
     4441 gtgctgatgc tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc
     4501 agatcagcga gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga
     4561 atagccaagc gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg
     4621 aaaatctgtt attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt
     4681 caggtcctaa cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg
     4741 gcaaggttat aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa
     4801 attatgaaga caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc
     4861 aagcattttt gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga
     4921 tcgagcagat gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc
     4981 cagctgaact atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac
     5041 aattagaaca actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa
     5101 ccttcctgga taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat
     5161 ctcaagaaca acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc
     5221 tttatgtaac gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg
     5281 cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag
     5341 tagatttgcc tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata
     5401 cagcacaagc agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag
     5461 aaatggattt tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac
     5521 gtttagcgtt cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga
     5581 tcgttccttt attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc
     5641 ataatgcctt gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct
     5701 ttacattcat ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta
     5761 tttaaataaa atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac
     5821 aattcttttg ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag
     5881 tattgaaatt ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga
     5941 aaaagaggga taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga
     6001 agaaacagta tatccgccaa atggcatcca atggctttgt cggtattttc acttccatgc
     6061 atataccaga agatgatgtt tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag
     6121 cgaaaaagaa taaactagaa ttgatggtcg atatttcagg agaggcattg gaacgagcgg
     6181 gtttttcatt agaagatcta acgcctttga aagcaattgg tgtaacaggt ttaagaatgg
     6241 attatcatat ttccaatcag cagattgccg actggtcaca tcaattgaaa atcagtttga
     6301 atgctagcac gatcacgcct aaagatatcg atgaattgaa agaagcagaa gctgatttct
     6361 cacagattga agcttggcat aattattatc cacgtcccga aacagggctg gacaaagaat
     6421 ggtatcagaa aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac
     6481 caggagatac agagttgaga ggcccactat atcaaggatt gccaactttg gaagaacatc
     6541 gagggatcca tcctcttgca ggcgctctag atctattagc aagtaacacg gatattgttt
     6601 acatcggaga tgcaggtctg tcagaaaacg ttcaggagca atttgcttct ttccaaaaag
     6661 aacagacagt cttgctccat acggaacctg tagatgaaga attttacgaa tatatattag
     6721 ggaagcatac gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt
     6781 ttcgagagat tccgcctatc ccagcaagaa atacagctac gcgtatgaaa gggtctatta
     6841 ctttagataa cgaaaagtat ttgcgctata tgggtgagat ccaattaacc aaatatgatt
     6901 tgccagcgga tgaaaaagtg aatgtagtag ctaaagttat caaagaagag ctaccattga
     6961 tcaaccaaat caaagctggt atgaactatc aatttataag aaaagaagga agataaaaat
     7021 gagcaaaatc aatttagaga acctaacaac ggaacgcaga aaccaagaaa cttttggatt
     7081 agatgagatg agtatttctc aagccgttca actaatgaac aaagaagatc agaaagtggc
     7141 cgctgcggtt gaaaaagaac tgacaagtat cgaaccagtg attgctaaca cgattgagtc
     7201 ctttaaaaaa ggtggacgtc tgatttatat gggagccgga acaagtggaa gactaggtgt
     7261 gttagatgct gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg tcataggtct
     7321 aattgctggt ggagaagaag caatgactgt agctgtcgaa ggggccgaag attcttatga
     7381 gctagggcaa gaagatctaa tcaagctgaa tttgacaaaa aatgatatag ttattggtat
     7441 cgcagctagc ggtcgtacac catatgtgat cggtggatta gattacgcaa aatcgatcgg
     7501 tgctgtcaca ggaacgattt cttgcaataa acaagccgaa atcagtaaac acgcggattt
     7561 cccaatcgaa gtcgatgcag gtccagaatt tttgacaggc tctactcgat tgaagtcagg
     7621 aacagcacaa aaacttatct tgaatatgat ttctactatc agtatgattg gtataggtaa
     7681 agtattcaat aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc
     7741 aaaacgaatc atcatgcaag cgacagaagc tgattacgaa acagctgaaa agtactttga
     7801 agaagcagat caaaatgtta agctggctat cgtcatgatt ttgacaaata gtgataaaga
     7861 ggaagctcaa gcaaaattag ctgaagcaaa cggatttgta aaagggacct tatgaggggg
     7921 aaaagaccat ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa atttataaag
     7981 gggcaggcgg catgggcaat gtagaaagca ttgtccactg catgactcgt gtgcgtatga
     8041 agatcatcaa tgatgatctt gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg
     8101 gtgtagtaga agatgaacag cttcaagtca tcatcggacc tgggaaagtg aacaaagtag
     8161 cacagcaaat ggttgatttg gcaggcgtga aattaggaga aaaactttct tctgtaagta
     8221 cagctacagc aaattcatca gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa
     8281 tgaaagcggc acaaaaatcg aaacaaaaac catctaaaat caaatcggta ttaaaagaca
     8341 tttctaatat ctttgtgcca atgattcctg catttgtcgg tactgggatc gtggcaggga
     8401 ttgccgcagt tttagcaaac ttagtcacag ccggaacact tgatgcagca acatggcaac
     8461 aatatatcga tgtgatgaac atcttgaaaa acgggatgtt tagctaccta gttatctata
     8521 caggtatcaa tgctgcacaa gtttttggtg caacaccaac tcttggtgga gttatcgggg
     8581 ccgttgtgat gctgacaggg atgaatccgg aagcaccact gaaaaactta tttactggtg
     8641 aagctcttgc agctggacaa ggtgggatcc taggtgtcat cttcgctgtt tggctattgt
     8701 caatcgttga gaaaaaactg cataaagtag tacctgatgc ggtagatatc atcgttacac
     8761 caactctttc attgattgcc atcggtctga ttgaaatttt cttcatcatg cctttagcag
     8821 gtttcatttc tgatggcatg gtcggtggga tcaactgggt acttggcgtt ggtggagcat
     8881 ttgccggctt cgttctaggc acattattct tgccgatggt tatgttcgga ctgcatcaaa
     8941 ttttgactcc aatccatgtg caaatgattg atgaaactgg tcgtacgcta ttgcttccaa
     9001 ttcttgcgat ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg attcgctgca
     9061 aaaaagataa agaattaaca gaaatgatca aaggagcttt accagtcggg atcctaggaa
     9121 tcggcgaacc attgatttac ggagtaacat tacctctagg acgcccattt attactgctt
     9181 gtatcggtgg aggtatcggc ggggcagtga tcggaatgat cggaaatgtc ggggctatcg
     9241 ctattggacc ttcaggagcc gctttgatcc cattgatctc tgacggtaaa tggttaggct
     9301 acatccttgg attattggca gcttatgctg gtggcttcgt tgctacattc ttcttcggta
     9361 ttcctaaaga ccgtttagaa aaagaagaat tagcgactga aacagccaca gtaaatgaag
     9421 tgcctgaaaa agtagtacct caaacttcag ctgaaagtgt gactatcctc tcatcagttg
     9481 ctgacggaac agttgtttct ttagataaag caagtgatcc agtttttgca caaaagatga
     9541 tgggtgaagg ctactttgta gaaccagaaa acggacatat ctattctcca gttactgggg
     9601 tagtcagctc tgtcttccca acgaaacacg ctattgggat cacaacgcca aatggtttag
     9661 aaatcttgct tcatatggga atcaacacag ttgacttagg cggtacgcca tttgatgtga
     9721 aagtaacaga agggcagcaa gtgacacctg atacgcttgt agcagaagct gacttagaag
     9781 cgatcaaagc agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg
     9841 taaaaaactt tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct gtgatggatg
     9901 tcaaagctta atatgataga aacaatagaa acgaaagaag aaactgaaat ctatttttca
     9961 gcttcttctt ttttattatt tattgttttc tgctaaaatc tgataaattc gtgtcacatc
    10021 agatggtgtt ccccgtaatt cataatctgc caagaaagga aagccaaatg tttgtgcata
    10081 ttgtttggct gttagacaat actgattatt gaagttttta tttccgctac cgactacacc
    10141 aagacaaagt ttatgattgt cttcatgttc cagatattca cgcatcgttt cggtcaagat
    10201 ttctgtatca ccgttatcta aaccatttcc gccgtctaaa taagtaggaa cgaaagtaaa
    10261 aaatggttct gtttctttcg taaaatcact attttcatgg atttctttta aagtaacttc
    10321 aggattgaca ggatcttctg catgcatttt ttcagcatat tctgctagat gtttggcaaa
    10381 ggcacgcgta tttcctgaaa tcgatatata taaaatgttc atggatcatc ctccaattct
    10441 ttccttttag tatagcatct tttttaagtt ttttggtggt gtaatttgga aagggactag
    10501 gcgctttgct tgtataatga gaaaaaagcc ggtagaatta tatttctatt aaagttttta
    10561 ttaattaagt aaaaataaaa aaggtttgtt atactttttg ataaaagcgg ttttattttg
    10621 gaggatgctt acatggatac agagcaacag aaaaaaggaa ttggtttaat tccacttgct
    10681 gccttagtaa ttggttcagc gattggaggt ggcgtatttg gtattatgac ggacatctcc
    10741 ggatcggctg gcggcccagc tattttcagt tgggtactcg taggaacagc aatgttgatg
    10801 ctctccttgt cgatcaataa tatcaataaa aaacgtccag atttaaaagg cgggatcttt
    10861 agttacgcag aagaaggttt tggccgtttc gctggtttta tcagcggttg gggttactgg
    10921 ctcacatgct ggttaggcaa cgtagctttt gcaacattgt tgatgagcgc attaggatat
    10981 ttcttcccag tatttgaagg tgggcaaaat ctggcatcga tcgtgatgag tacagtcttt
    11041 ttatggggat atgcatggct agtgaatcgg ggagtcgaaa atgctagttt cgtcaatgct
    11101 atcgtcacat tctgtaaatt agtgccatta tttttattta ttgtagtagc tatcagcacg
    11161 ttttcgatcc aacaattcat tgataacttt tctagcgaca ttatattgaa tgcgaatgga
    11221 cagccgatcc cgttttccag tcaagtattg agttgcatca tggtgatgct ttgggtattt
    11281 gttggtattg aaggagcctc tgtcgtaggt tcacgagcaa aaaaacaatc agatgttggg
    11341 aaagcaacga ttctagggat attcggcttg attattattt acgtcttggt ttccatgctt
    11401 ccatatggga caatgacgat gaatcagctg caaacgatca aggcgcagcc tgctatgggc
    11461 tatgtgtttg aaatgatggt tggtaaatgg ggagcagtcg tgatcaacgg cggtttgatc
    11521 atttctttag ttggtgtttg gctctcatgg acgatcttac cgattgaaac aatgggaaac
    11581 atggctgaag acggactttt gcctaaagca tggggaaaac tcaacaaaaa tggggcacca
    11641 acttatgcta tcgtattgac gacgttatgt acgaatgtct ttttactttc attattattc
    11701 actgacagtg cttataactt tgcttacaca cttggtacag cagctatttt cttcacgtgg
    11761 cttttcttag gattgtacca aatgaaactt tcttatcagc ggaaagaatg ggtccaattt
    11821 ttagtagggc ttttagcaag tgcctttcaa gtatgggcta tgctagtcgt agcctttaat
    11881 gaagtaatgc ttgttcttgt tcttttcttg ccaggcattt atttttacct caaagcacgt
    11941 aaggagcaaa acgagcctgt acaacatatc cacttagata agattttact tataggtgtg
    12001 acgatggtcg gagtgattgc aatggtttta tttgccactg ggatgcttca cgtatagaag
    12061 tataaaagaa gtataaagag aggttgtgag cctgtcgttt attcaaaaac atactaggaa
    12121 aggagctgtg acacaaatgt tgtcacagct cctttttcag aataaacggt gtttaagaga
    12181 cagccaatcg gcaataaggc tgaacgtctc tttcacagcc tcttttttga tgagtcatca
    12241 tacatttttt cgcttaaata cgagatatcc taatgctaag aacaaaacga tgtaagctaa
    12301 attaccgaat agcatttgat tagtagaaag atgagtcaat tgcttcatga tctcttcgtt
    12361 tccaatttgg ttttgcagat tcaacatact gatcggattc catttgatcc actcccattt
    12421 ttggatgagt gcaataagga taccagatat gattgacgaa gcaaaataaa agacgattcc
    12481 tgctgagatg gatgcgccgg aactattgat aaagcaagca atcatcaaca ctaaacttaa
    12541 aatcagccat aaaccgatat aactacccaa agtataagtg aacaagcttt ggatcagtgt
    12601 ttgaccagta gaaaccttct ccgtgaagga aatcgaaggg aaaagaatca gtttcatcaa
    12661 cacagtgaca ataatagcta aaaggtataa ataaacagaa taaataacta atgtgatcca
    12721 tttgcttact aatatttctc ctcgagaata ctttcgataa aggagatttt tcaatgtacc
    12781 atattgtgct tccatcgaaa tgatcgtgct tgcagctgca atcatgataa agacaatcca
    12841 agatgtggca ctaaaaagct gagcagtcat ttcaactgga tcaatgatcg tcgtgtattt
    12901 tttggctaga agtgctgttc ctatgagcaa aacaactaat agaacagata aaacagcagt
    12961 tgactttttc ttgatcaatt taaataattc ttgttttaca agcaatgtca tttttcttct
    13021 tcccccttta ctgaatccag aatttccaat agagaagatt ccaaatcatt cgtcagatgc
    13081 tggacgtctg tcacatggat attttgtgca gacaatagct ggatcacggt tcctaataat
    13141 tcttcgtttt cttttaaaag cttgatttta tcaggatctt ccaaagtata tccagcttct
    13201 gtcaagatcg ttcgagcagc tagatcattt gttgtagaga taacgatgaa atcttgcgct
    13261 gtttggttca attcagccat tgtacattgt ttgatgatct ttccttgatt gatcaggatc
    13321 agctcatcga ccattttttc aagctcgctg agtaagtgac tggagatcaa gaaagaggtg
    13381 ccctgagtag ccaagtcggt aatgattttt cgcacatctc ttgttgcttg tggatccaaa
    13441 ccgttcatcg gctcatctaa aataacgata gacggatggt tcagtaaggc aagagcaatt
    13501 cccaattttt gtttcattcc caacgagtag gatttcgctt ttttgtgaat gtacttttcc
    13561 atcttgagct gattcacgat agtgtcgata gcagatacat cgttggtttc agagaataac
    13621 ttcagatgat catagcctgt taagaatgga tagatacccg ggtattcgat caatgctcct
    13681 acttgttcca accctttatg actagaggga gaaatctcat tttcaccaat acagattgtt
    13741 ccatctgagt aatggatcag tcctaaaata gctttcatga tcgttgtttt tccagcgcca
    13801 ttaggaccta ctaatccaac gattttgcca ggggataagc taaaactgac atcgctgagg
    13861 gcttgtttgt gaccaaagtt cttggataat tcttggaccg aaagactttt caaaaatatt
    13921 cactcctttt ctatagatag tacaagcata cacctgatga gtggagaagt cgctaggtca
    13981 ttggacttat ttcttatttt ttcataaaaa aattgaatat taaataaaaa ggcttatttt
    14041 ttctgatcag tacttattcc aagtgtctag tggccgctag taatcttgat gccaatgata
    14101 ctgatgagta gcatccccac aaagaaccaa gtggaaagat tcagcttatc atgaaacaag
    14161 accacaccaa ttatgaccga accaactgcg ccaatccctg tccagatagg ataagagaga
    14221 ctcatcggca aacttttgat tgcctttgac aaaaagtaaa aactagcaat catgccaatc
    14281 actgtgtaaa aagaataatt gattttagaa aaaccatcac tccatttcat catcgttgac
    14341 cagaatactt ccaaaattcc tgctatgatt aattccatcc atgccatact tacattcctc
    14401 cttaactata aaaaaataca caaaaagagg aaaatcatcc cttcaaagac ctaatggaat
    14461 gatcatcctc tttatcctac ccggtggcag attgtatatc tttttctgta gtatagcaca
    14521 cctatccaga tatcggcaat ctcatacttt ttagtaaagt gtaccaagca ctgacggata
    14581 tatcgttagc ggaaaagaag atgaatgctt tgcaatttca tagaaagaag ttgttattta
    14641 ttaatcattt aaaaaaaaca gtaactagac ct
//
LOCUS       DAZKAS010000065        14607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000065 DAZKAS010000000
VERSION     DAZKAS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.067"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..1940)
                     /locus_tag="ACG8H7_001636"
     CDS             complement(9..1940)
                     /locus_tag="ACG8H7_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFP8983734.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1958..2872)
                     /gene="pfkB"
                     /locus_tag="ACG8H7_001637"
     CDS             complement(1958..2872)
                     /gene="pfkB"
                     /locus_tag="ACG8H7_001637"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP8983735.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2869..3621)
                     /locus_tag="ACG8H7_001638"
     CDS             complement(2869..3621)
                     /locus_tag="ACG8H7_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP8983736.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3780..3989)
                     /locus_tag="ACG8H7_001639"
     CDS             complement(3780..3989)
                     /locus_tag="ACG8H7_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983737.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4000..5328)
                     /locus_tag="ACG8H7_001640"
     CDS             complement(4000..5328)
                     /locus_tag="ACG8H7_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983738.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5464..5619
                     /locus_tag="ACG8H7_001641"
     CDS             5464..5619
                     /locus_tag="ACG8H7_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFP8983739.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5693..6280)
                     /gene="yihA"
                     /locus_tag="ACG8H7_001642"
     CDS             complement(5693..6280)
                     /gene="yihA"
                     /locus_tag="ACG8H7_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP8983740.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6363..7613)
                     /gene="clpX"
                     /locus_tag="ACG8H7_001643"
     CDS             complement(6363..7613)
                     /gene="clpX"
                     /locus_tag="ACG8H7_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFP8983741.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7860..9149)
                     /gene="tig"
                     /locus_tag="ACG8H7_001644"
     CDS             complement(7860..9149)
                     /gene="tig"
                     /locus_tag="ACG8H7_001644"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP8983742.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9303..10235)
                     /locus_tag="ACG8H7_001645"
     CDS             complement(9303..10235)
                     /locus_tag="ACG8H7_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983743.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10392..11051)
                     /locus_tag="ACG8H7_001646"
     CDS             complement(10392..11051)
                     /locus_tag="ACG8H7_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP8983744.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11054..12079)
                     /gene="sppA"
                     /locus_tag="ACG8H7_001647"
     CDS             complement(11054..12079)
                     /gene="sppA"
                     /locus_tag="ACG8H7_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP8983745.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12092..12382)
                     /locus_tag="ACG8H7_001648"
     CDS             complement(12092..12382)
                     /locus_tag="ACG8H7_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983746.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12791..14596
                     /gene="glmS"
                     /locus_tag="ACG8H7_001649"
     CDS             12791..14596
                     /gene="glmS"
                     /locus_tag="ACG8H7_001649"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP8983747.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         4162 a         3092 c         2543 g         4810 t
ORIGIN      
        1 ttttatttct atgcttctgc tactgcagga gcttttctca ataaaccata aatcacacca
       61 gatacgactg ctccgatagc aataaacacc aaatacatga ccgggtgact cagtaagaat
      121 acaacgaaaa tcccgccgtg aggagccagt aatttgattc cgaaagctcc gactaatccg
      181 ccagttaaag cagaaccaat aataaaactt ggaatggcac gaatcgggtc agctgctgca
      241 aatggaatcg atccttccgt gacaaacgat agtcccatca cgatgttcgt caaaccagca
      301 tcttgatctg ttttgctgaa tttattctta aacaaacgtg tagcaacgaa aattgccaat
      361 ggaggtacca ttcctccagc cataacagaa gccatcacga cacttccgcc agttgcaaca
      421 gtcgtagcta aagttgctgt accaaacaca taagcggctt tgttgattgg accgcctaaa
      481 tctgccgcca tcattccagc taataatgca ccaagtaaag cagcattcga accactcata
      541 cttgtcaaga aaccgttcaa tgcatcattg attgctttca tagggacatt gaccagcaac
      601 atcaagaaac cagtaatcag taagccaaac actggataaa agaggatcgt tttaattcca
      661 tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa atacgatgac ataaccagct
      721 aagaatccac caatcagtgc acctaagaat cctgcaccgc ctgtattagc taatgcacca
      781 gcagcaaatc caacgatcaa accagggcgg tcaccgatac ttgaagcaat aaatcctgct
      841 aataccggca gcatgaaacc aaatgcagct tgtccgattt ggttgaacca gctagcagct
      901 tgattgtaat tccctagatt tgataattga tcatgtggca cgccaataaa ttggtcgatc
      961 atgaaagaaa gcgcaatagc gattcctcca ccaataacga aaggtaacat gtgtgatacg
     1021 ccgttcatca aatctttgta aattctttgt ccgatagtac catccgcatt tccttcttct
     1081 gaactgcttt cttttccatc accatggaat actggtgcag taccactaag ggctagattg
     1141 atcaactctt ctggtttacg gataccatca ctgacaggac gattgatcag ttcttttccg
     1201 tcaaagcgat tcatctctac ttttttgtct gctgcaacga ttacaccttc tgcacgggcg
     1261 atgtcttcag cagtcaatcg gtttttgatt ccttctgaac cgttcgtttc gactttgatc
     1321 tccacaccca tttctttcgc ttttttcttc aaagcatctt ctgccatgta agtgtgagcg
     1381 atccctgttg gacaagctgt aacggcaaca acgaattttc tttctggatg agcagaagct
     1441 tctgcagctt gtgtttcttg ttctgctttt tcttctgctt ctttttgctc ttcagctgtt
     1501 tggaacaagt cttggacttc ttctggtgta tttgcttctt ttaattttcc tacgaaatct
     1561 gcatcaatca gcaatcgaga tagtgctgct aatgcttgca aatgtgtgtc gtttgcgcct
     1621 tctggagctg cgatcatgaa aaacagataa gttggctgtc cgtctaaggc ttcataatct
     1681 actccttttt ggctttttgc gaataaaact gttgcttctt taactgcact gtttttcgcg
     1741 tgaggcatgg cgattccatc accaagacct gtagaagttt gtgcttcgcg ggctagaatt
     1801 ccttctttat atgtttcgat atcggaaatc cggccggcat caaacatttt ttggaccatt
     1861 tcatcgattg cgcctttttt atcagtcgct tgcaaatcca tgatcatcgc ttctttgacc
     1921 attaaatcct tgatattcat gattatcctc cttgaaacta atcaattttt gtgatttcaa
     1981 cttctggcag caactcatca atcaattcgc gagctgccaa gtcatcagaa aaagctgttg
     2041 cgcttccgca agcaactccc catttaaagg cttcgattgg atctttcgta cggctgtaag
     2101 ccccaataaa gccagcaatc attgaatccc ctgcacctac tgaatttttc aatgggcgaa
     2161 ctaatacatt cgttttataa gttccttgtt ccgtgaagag caaagcaccg tctccagcca
     2221 ttgagatcag cacattctgt gccccttcag ctagtaattt tttcccatac ggaatgattt
     2281 cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa ttcgtgattg tttggtttga
     2341 tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc acctgttgta tcaatcacga
     2401 aatctgcacc tttttctcga atgatatcaa tcagttcttg ataaaatcct tttcgcaggc
     2461 tagctggcgc gcttcctgac aatacgacga tatctccttg tcctactgtt cctaatgctt
     2521 gcttcaactc atcgatttct tcatctgaaa tagatgggcc taagccattg atctcagttt
     2581 cagtgtctga ttttagtttg atattgattc ttgtatcttc ttttacggtt gtgaacaaag
     2641 actggatttg ttctttttca agccacttcg aaataaattc gccagtgaac cctcctaaaa
     2701 accctagtgc tgtagattga gaatcgatcc gtttcaaaat acgggaaaca ttgatccctt
     2761 ttcctccagg taacttgaag tcttccttca ttcggttcaa atcgcctagt tttaacccat
     2821 ctacgcgtac gatataatca attgatggat taagtgttac tgtgtaaatc attgtattgc
     2881 ctccttaatt gttgtctttt cacggaattc attaacgaat tctaatggac aacgctcggt
     2941 tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag gacaactggt tgaatttggt
     3001 atgatcaatc aatacaaatg tctcttcgga atttttcatg gctaaacgtt taacagctgc
     3061 ctcttctgga tccggagtcg tgaatccttg ttcaagatgg gcaccgttca ttcccataaa
     3121 tgctttatta aaatgaaaat tttttagttg ttctatcgca ttagatccta aaacagcatt
     3181 agttgccagc tttatcgttc cacctaaaat aatggtagca agctccaaat caactaaatt
     3241 tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt tttccttgca gaaaaggaat
     3301 catttctaac gtagtagagc cagcgtctaa ataaatgaca tcttcgtcat taatggtttc
     3361 agctgccgat ttggcgattt gctgtttttc gtgaacgttt ttgagtgatt tttcactcat
     3421 attctgctca aaattcaata attgctcttt cttagctccg ccatgaactc tttctagaaa
     3481 cccactattc tccagctctt gtaaatcacg tcttatcgtt gattcagaag cattcaaaag
     3541 gtttacaaga tcttgtgatt tcacgatttt ctgctgttca agcaagtcta ggattttttg
     3601 tctgcgttcc tctgtaagca ttttcacgat cacctcaata caaataatag catatttttt
     3661 ttctactagc aatataaatc agtcaaaaac gttcataaaa attataaatc agtcaaaaaa
     3721 aaataaaaaa ccagtcagaa agactggttt tttatttttt tgtctatcat caagccgttt
     3781 tatcgttttt tattttcgct tcttgttttt ttcatataaa cagaagcagg ttttctttcc
     3841 ttatgaagaa gataataata aagtcccatc ccgccaaaca tgaccaaaaa accgatgact
     3901 gatcgaatag gaaaattagg aacaaaaaag agacagacgg ataataatga tgccggaacc
     3961 attattcgat aatagagttg tttatccatt ttcttcacct catatttttt gtaaagtaat
     4021 caattgttct aattctcggt aatctaaaaa ttgcacacgc agatgctgct cacctttctt
     4081 gacatcgaat tggatacttc gccgcttttt ctttattcgt ttgaaggtaa catcagagag
     4141 tctatgtttg atttccttta acggtaattc tgacagggat tctgaaggaa catagattct
     4201 taaggataaa agatactctt gatatttttc atctgttacc tctacttcta gatattccct
     4261 aatataataa caataagctt cgattccatt atccaaagca acagtcggat cgataacttt
     4321 atagcacatc atgcgtgatc gattctcgtc aacccaagga aaatacgcct gagcatttag
     4381 tgttccgcga acacgccttt ctttataatc tccttgttgg atatcataac tttttgttac
     4441 aaaaaatgaa gcattctttt taggaacagg ttgcatttgt gcattctgat actggacaga
     4501 ttggatcgta tggtcagtgt aaatgtattc aacgaatggc ggtttcaata aaccgacacc
     4561 attgtcccaa gcaagatgat caaacccaag ctggtcccta aaataggatt gaaacatttg
     4621 attgtttggc ttttgccagt taaaggggtc tcgataaagc tggatacata tgacttcgtt
     4681 ttctaattcc ttcacttttt ctacttgttg gatgtcgaga aaaatcggtt tagggatgat
     4741 ttgatgataa tagcgactat aatacatttc ccaacacgaa gtgaaacata aaccttggta
     4801 aaaaagatcg tatcctggaa attggttgca atcgactact acttccccgt tttgttgttt
     4861 cattttaggt aaatgttcga tttcatcctt tttctcaaac aatagccgtt gagagatctg
     4921 ttttgtattg tgatagataa actcgctata agctcgcata taggcgaaca ccccatgagc
     4981 aatcattttt ttctgaatga agtctcgaat caaaaattgc ttttctttga aaacagagat
     5041 agacaataca ttttttatca ataattgatt atcttccaag cgaaaactac attcgttctc
     5101 gtcatttcct acaactaacc aactttcgtc tcttaccgat gctagcatta ttttttcttg
     5161 aagctttcga agatcatacg gatccgcttg ttgtgtgaaa gagcggataa atgttggctg
     5221 gatcaacaga cgtgtcaaaa tagcccaatc gcttatttcc tgtttttttt cttcttcatt
     5281 taagtctgtg agctggatag tcatcaataa tttatcttcg ccaatcattt tctcacctct
     5341 ttcaaatctt tttataattt aattataacc aatgataaag aaaaactaaa actttatgtc
     5401 ttagaaacca atttggacga ttacatgata gactaagtaa aaagaaacaa agaggtgttt
     5461 acaatggggt tgaaatttga acgtacagat aatttagata aacttttcga atcatttgct
     5521 gtcgatccgg aaaaaacagt tgcaccaaaa gaagagatcg atcgatttct aaaaccacag
     5581 ccacaaaatg aaaagaaaaa agacaagaaa caaaagtaag ttaagctcaa aaaaagactt
     5641 accagccaaa cttttatgaa agagtttgac tggtaagtct tttttattct ttttatgatt
     5701 caattgcttg ttcgattgct gcccacgctt cttcttttcc ctgttttgta acggaagaaa
     5761 agagaataaa cgtgtcattg cgatcaaaat ccaacgcttt tttgatttcg ctctcatgct
     5821 tgttccattt accacgaggg atcttatctg ccttagtggc aacaacaatt acagggatat
     5881 cataatactt caaaaattga tacatctgga tatcttcttg agaaggctta tgacgcaaat
     5941 ctactaaaga aacgaccgca cgtagctggt ctctagtcgt taaatatgtc tcgatcatct
     6001 ttccccattt tgctcgctca gttttggata cttttgcata cccatatccc ggaacatcga
     6061 caaaatgtaa tgcgttctcg atcaaataaa aattcaatgt ctgggtcttt cctggcttac
     6121 cggaagtacg ggctaaattc tttcgatcaa tcaatgtgtt gataaaagaa gatttcccta
     6181 catttgatcg tcctgccaga gctatttctg gcaattgtgt atctggatat tgttttggtg
     6241 ccactgcact tatgataatt tcagcttgat ggactttcat ggtctctctc cttaacaagg
     6301 tgatgaataa agcaagcggc tgtcgcaaaa gccaaaatga cttttacggc agcctctttt
     6361 catcaacccg cttttttctc tgaatcttta tagattactg ttggctcacc agacaattca
     6421 gctgcttctt ttgtgataat cactttttcg atgttttcat ttgaagggat atcaaacatc
     6481 acatccatca tgatttcttc aatgatggaa cgaagtcctc gagcaccggt attgcgttcg
     6541 attgcttttt tcgcaatggc tctcaacgct tcaggttcga attcaagttc ggtattatct
     6601 agagaaagaa gtttttgata ttgtttgact agcgcatttt tcggctcagt caagatccgt
     6661 accagatcat ctgttgtcaa tttctctaaa gctgccataa caggtaatcg tccgataaac
     6721 tcagggatca atccgaattt tagtaaatct tcaggaatga tatgctgcat gacactttcg
     6781 tcttcagaaa gtttttggtt ttgtgtacca aatccaatcg tcttttcgcc catacgattc
     6841 ttcacaatcg tttcgatgcc atcaaatgca ccacctacga tgaacaatat gttcgtcgta
     6901 tcaatctgaa taaattcttg atgtggatgt ttacgtccgc cctgaggtgg cacacttgca
     6961 acagttcctt ctagaatttt cagcaaggct tgttggacac cttcgccgga aacgtcacga
     7021 gtaatagata cattttcgct tttacgcgca attttatcga tctcatcaat atagatgatc
     7081 cctttttctg caagttccac attgtaatca gcagattgaa gcaatttaag caaaatgttt
     7141 tctacatctt cccccacata accagcttct gtcaaacttg tcgcatctgc gattgcaaaa
     7201 ggtacattca atgtttttgc taatgtttgt gctaaaaatg ttttaccaga ccctgtaggt
     7261 ccgatcaagc agatattgct tttttgcagt tcaacatcat ctgtgttttc ttgtgcttgc
     7321 tgattgacac gtttatagtg attgtatacg gcaacagcta acgagcgttt tgcgcgatct
     7381 tgtccaatca cgtagttatt aaatacttca agaatttcta gtggtttagg gacttcagtg
     7441 aattcgcgta cagcttcttc ataaaattct tcatcaatga tttctttaca taggtcgata
     7501 cattcattac agatgtagac ccctggacca gctacgatct ttttcacttc ttcttgggtt
     7561 ttcccgcaaa aagaacagcg tacggtctca ttgctacttg gattgtcata catggtcttc
     7621 accccttaac ttgcaaaaag tatgcaagaa actatcaaag actttacagt cttttactat
     7681 cataacatag tttagtaaaa agaaaaagtg tttaacaaag gatcaactga cctatacctt
     7741 tgcatttttt actcttaaca aaaaataagg aactgtgaca aaatcgtgtc acagtccctt
     7801 atttcgaata aacaattttc tagaagtagc ttcttcagaa atgatttatt acttgatttc
     7861 tattctttcg cttctgtcgc tgtacctgtg atcaattcga ttgctttttt cattgtcaca
     7921 tcatgttcca gcatatcttc tgtcaacacg cgtttgattt gttcgattgg catgttgtat
     7981 gtttctgata attcttggat ttctgcattg atttcttctt ctgttgcttg aagattttca
     8041 gcagctgcaa ttgcttcgat cacaagattt gttcttgtgc gcatttctgc ttcaccttcg
     8101 aattgtttat gcaagtcttc ttctgtagaa ccagttaatt gatagtacat ttcaggagag
     8161 ataccttgac gttgcatgtt gttcaagaat tcgtccattg aacgatgtac ttcatcatga
     8221 accatcacgt gtggcaattc aacgatttct gcgttttcaa cagccatacg gatcgctgct
     8281 tcatcttttg catcgtccgc tgctttttct tttgtttcta ctaattcttt gcgatatttt
     8341 tctttcaatt cgtctaatgt tgatacttcg tcatcaacat cttttgcgaa ttcgtcatct
     8401 aattcaggca cttcttttgc ttttacttca tggattgtca ctttgaagac tgcttctttt
     8461 ccagcaagat cttctgcttg ataatcttct gggaaagtta catttacatc tagactttca
     8521 cctgatttat gaccaactaa ttgatcttca aaacctggaa tgaatgagcc tgaacctaat
     8581 tcaagtgagt aattttcgcc ttttccgcca tcaaacgctt cgccatctac aaagccttcg
     8641 aagtcgatta ctactgtatc gccattttca gctgcagcat cttctttgat aactaattca
     8701 gcttgtgctt cttgttcacg tttgatacgt gcatccacgt cagcatctgt tacttcacgg
     8761 tcttgttttt caacaactag atctttatat tgtcctaatt ttacttctgg tttcactgtt
     8821 acttcagcag tgattaccca gtcttgacct ttttccatgc tttcaacatc gatttttggt
     8881 tgagaaacag gatcgatgcc agcttctttg acagcagctt cataagcttc tggtaataca
     8941 gcattcaatg catcttcata taaagcttct tcaccgtaca tacggttgaa tacttgacga
     9001 gaaactttcc ctttacggaa tccaggtacg ttcaagcttt ttttcacttt attaaacgca
     9061 gttgttaatc ctttttggat ttcgtcttgc gcaatcgtaa attttaaaac accatcattc
     9121 gtgccttttt tttcaaatgt tgcagtcatt taattccctc cgagtttaga aattcatttt
     9181 atacatcaaa aacgtttcat gcataccttt aaatagtaca ctaacccttt caaattgtaa
     9241 acatattgaa tggatagaat ccttgttttt cataacattt ttatcttttt cactgataaa
     9301 atctaatgga agaagatcat ttgctgcaaa attttttctc ttttttttaa aaaatcttgt
     9361 ttttcatctt ctgtaccttc atacatcgtt tttcctgtac acgcctctac agtcagttga
     9421 aaccagcgct tcgggtcttc gatgtatgta tcatagacag gataaacaat tgcagattgc
     9481 aacgtgaatt cttcttttaa cacggaacta agcagcgcat cttcattccc gtaacgatct
     9541 gaaaatacac ataattgctg gtaacttgcc tgctgctgcg gctttggcag atcttttggt
     9601 atgactttga ctaacttttc ttcaatcgtc aaatagaaaa caggatggca ttcgttcaat
     9661 tgcctaagtg tctccagtaa ttttgccctt gctaaaatat ggacctctgg cgatacaata
     9721 agtttactcg ctgtttggat aaactcatga taaggaagct gttcgatctc atcaatagac
     9781 gcaagctgac gtgcaggttc caacgaagga agttcctccg atttattgtg gagtgccttg
     9841 atcttctgcc tctccatttg tccggataat tcttctgcaa attggatttt ttcaatgacc
     9901 cgctgctcga aagcttcata aaaatctgta cttgctatca attttctagc atataaaaaa
     9961 tcttgtgtac tgattaacaa gtcaaaataa aattcagcaa actcctcatt ttgaagatac
    10021 tgtttttcat gcaagagtgc ttgcttcaat gcttcttttt tctcgtccaa ctcgtaaaga
    10081 caaaaaacaa gcaggctatt agcctcaaag ctttctttca actcgtaagc tcgttgaaat
    10141 aattcttttg cctgctgata ttgctggttt gctagtgctt gttgtgccac tcgcattaaa
    10201 ttctcatatt tttctggaaa atcaatcggc ttcacgcagt gtcctcctct tgctcatatt
    10261 cagctcatgt tcaatagtag caaaacaaaa aaagcatgac aattaaaaat aactgaagca
    10321 ccctctaaaa gctagatttt agatctgact tttagaaggt gctttcaact gttactgctt
    10381 tttattgctc atcagctgtt gacttcctgg ttgaacgctt tgatcagatt gatcgtaaca
    10441 acacttgtat tagtgaaaaa atcgcccaga tgttgctttt tctgattaaa gactgctgga
    10501 agatacccta acatcagcaa aggaaatttt agaatgaacc ggcaagcacc ttctctaact
    10561 aaaacggttg accagctaag ttcttcttca tcaaagctga tgactcgaat accaaagatc
    10621 attttcccaa tcgtttggcc atgattgaat ttcgtcaata aaataaaata cgctagataa
    10681 ataagtaagg ctgtgaatcc gtagagactc agataagaat ctgaaaaatt aagccaacct
    10741 agatttttgg caataccaag tgttaaacgc gtaatagctc cgatacacaa aagatcgacc
    10801 aagaaggcaa acattcggat aaaaaaacct gcatagaaat acttaggaaa atcatttgct
    10861 tttggcggtt catttcttgt gtccgtatac tttttccatt tacgttgttt ctttttgatt
    10921 tcttcgggcg tcaaaggctc tctttcaaaa gagctcagta ccttttggga aaagttggag
    10981 tcaggtccta cagcaggctg ctgttgctta tctttttctt gttccttatt ttcatttgaa
    11041 agctctgaca tgcttactca cctccgtaat aatacattgc ttttggtgat tccgctgtcc
    11101 ctagattctc aagaacggat aagatctgac tagtctcaga aggcttcaat ccctggaatt
    11161 ctgctagttt acttcctaac catgtactag caaacccaga tggcgtggaa gaatattcta
    11221 ctagttccgc atcttcaagc tgttgttcct ttcgtatagc ttttaaagca tcttcaggaa
    11281 atcccaattc atcgactaaa cctacttctt ttgcttggac accatcataa atccttccat
    11341 cagcaatttt tttgacttct tcttcagatt tattccgtcc ctcgctgacg attgctacaa
    11401 aacgactata tgcgctgtcg atatatgctt gaagcaccgc ttcttcttct tttgtctctg
    11461 gtcgcgttcc tgaacccata tctttcaacg ctccgctttt aactgttgta tcttccacac
    11521 ccagtttttc tagaagtcct gaataattca atcctgacat gatcacacca attgagccgg
    11581 tcactgtttc ttctgttgca aagattttat ctgcttgagc agaaatatag tagccaccgc
    11641 tagctgccat gtttttcatg ctgacataca ttgggatatc atgttcttta cgaatcgtat
    11701 ccaatttctt agcgatttcc gcactttcgt agacaccgcc accaggagaa tttacttcta
    11761 aaaagatacc tttgactgtt ggatcttctt caatcgcctt cagttgttcc ataaaatttt
    11821 tatgattgta accttcagaa gcaaataaac ctgaagaccc tcccgcagca atcgttccat
    11881 ctactgataa ttttacgatt ttttgcgtac tgtctccctc ttccaacaca tttggagcta
    11941 gttcttcatc cccatataac caactgttca atccctcaat ttgatcgttt tcctgaggct
    12001 tagtcaaact agctgaaact aacgaaacca caaataatcc tgcagcaatc agaactgctg
    12061 cccaacgtct tttattcatt ctttctcctt tctaatacaa gttttttatg acgatcacag
    12121 tttctgactg ctcagaagtg tcgaaccgct ctgctttttc ttcccgcgca tacccggaaa
    12181 acgactcttt tgttaattca tcttctaccg taaactcacg agcttgagca acttccaagg
    12241 catcaggtgc gatttcttgg atgctatccc agccagtatc taaaaatatt ttcgtatcat
    12301 ctgatacacc agctgatgct tcgaatttgg acaattgttt ttttaacatt cccagtgtca
    12361 tgatctcaat ttttgaatcc atttttactc ctcctctatc cgattacttt ctattgtacc
    12421 gaactcctga aaaagatgat agataaaaac ccattatttt ttgacataaa aaggacattt
    12481 gacaaagaat ttgatatata ccaatttatt ttttattctt tttttgttga caagaacatt
    12541 cgcaaaaggt tatgatttta aatgtttcca gataaaaaac aaaaaggagg atagttatac
    12601 cttttaggat agcgccaggt ctgtgtaaac agatgacgag gagagggttt atcgaaagat
    12661 tcggcggatg gccctaggga ctgcactcta cagaaaatcc acaaaaaatg tctgcaaaga
    12721 taaaacaaaa ggattttcct gcagctggaa gcattcatac gaattcatta tagaaagtag
    12781 gatgtttttt atgtgcggaa ttgttggaat gattggatta aaaaatgtaa caccaggatt
    12841 gattgacggt ctagaaaaat tagaataccg tggttatgat tcagctggta tctttgtctc
    12901 agatggcaca actgattatc tggtcaaagc acaaggcaga attcaaaatt tgaaaaacaa
    12961 aataaatatt gatactacag gaaatatagg tattggtcat acacgatggg caactcacgg
    13021 gcaaccttct gaagaaaacg cccatccaca tacttcccaa agtggacgct tcgtgctcgt
    13081 tcacaacggc gtaatcgaaa actttgaaga acttaaaatt gcttatttag caaatgacca
    13141 ctttattggt gagaccgata ctgagattat cgctcatcta attgaaactt tcgcgaagga
    13201 tactacaacg caagaggcgt ttttaaaagc tttgcgggtc atcaaaggtt cttacgcttt
    13261 cgctttgatt gatcggacag cacctgatgt tatttacgtc gctaaaaata aaagtccact
    13321 attaatcgga ctcggcgatg gattcaatgt gattgccagc gatgcaatgg ctatgcttgc
    13381 tcatacgaag gaattcgttg agattgaaga tgaagagatg gttaccgtca catcagaaaa
    13441 agttattatt caaaattttg caggtgacat cgtagaacgt tcttcattcg aagcacaagt
    13501 agatgcttca gatatcgaga aaggtaccta tcctttttat atgctgaaag aaatcgacga
    13561 acagcctatt atcatgcgtc gtatcgctca aaaatacgta acagaagaca atcgtgtctc
    13621 tcttgatcaa aagctggtcg atacattgtc tgatagcgat cgaatctata tcgttgcttg
    13681 cggaactagt taccatgcgg ggttagcagg taaacagact ttggaaaaat tgacacagat
    13741 tcctgttgag gttcatttag ccagtgaatt tggctataac acacctcttc tttcaaaaaa
    13801 accattcttt attttcttga gtcaaagtgg cgaaacagca gacagccgcc aagtgctggt
    13861 aaaaatcaac cgtctcggct acccttcact tacgattaca aatgttgctg gttctactct
    13921 ttcgcgggaa gcatccttta cactgctatt gcatgctgga cccgaaatcg cagtagcatc
    13981 aacgaaagct tatactgcac aaatagctgt actaacttta ttagccaaag ctatcggtga
    14041 taaaaaagaa tttgaaactt ctcttgtatt cgatatcact catgaattga gtttagttgc
    14101 taatgcgatg gaatcggtca tcgcccaaaa agattattta gaagagttgg cagaagtata
    14161 tctcagcgat acaagaaatg ccttttacat tggacgtggt gctgactaca acgtttctct
    14221 tgaagctgct ttgaaattga aagaaatcag ttatatccag gcagaaggtt ttgccgctgg
    14281 cgaattgaaa catggaacga ttgctttgat cgaagaagga acaccagtga tcggaatcat
    14341 cagtgaagaa gtgactggtg cgcatactcg aggaaacctg aaagaagtcg aaagccgtgg
    14401 cgctaaaaca ctagttattg tctcagaggg attagaaaag gaaagtgacc agctggtgct
    14461 accaactgtc cacccttatc taactactct aaccacagtc gtaccgactc aattacttgc
    14521 atattacgca acgctattac ggggattcga cgttgacaaa ccacgtaatt tagcgaaatc
    14581 agtgacagta gaataataaa agaacaa
//
LOCUS       DAZKAS010000066        14512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000066 DAZKAS010000000
VERSION     DAZKAS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.068"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..825)
                     /locus_tag="ACG8H7_001650"
     CDS             complement(130..825)
                     /locus_tag="ACG8H7_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFP8983748.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="ACG8H7_001651"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="ACG8H7_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP8983749.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="ACG8H7_001652"
     CDS             complement(2024..2215)
                     /locus_tag="ACG8H7_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFP8983750.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="ACG8H7_001653"
     CDS             complement(2236..3126)
                     /locus_tag="ACG8H7_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP8983751.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="ACG8H7_001654"
     CDS             complement(3113..3880)
                     /locus_tag="ACG8H7_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP8983752.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="ACG8H7_001655"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="ACG8H7_001655"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP8983753.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="ACG8H7_001656"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="ACG8H7_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP8983754.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="ACG8H7_001657"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="ACG8H7_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP8983755.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8451..8834
                     /locus_tag="ACG8H7_001658"
     CDS             8451..8834
                     /locus_tag="ACG8H7_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983756.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9002..9760)
                     /gene="jag"
                     /locus_tag="ACG8H7_001659"
     CDS             complement(9002..9760)
                     /gene="jag"
                     /locus_tag="ACG8H7_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFP8983757.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="ACG8H7_001660"
     CDS             complement(9778..10596)
                     /locus_tag="ACG8H7_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFP8983758.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="ACG8H7_001661"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="ACG8H7_001661"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP8983759.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="ACG8H7_001662"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="ACG8H7_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP8983760.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="ACG8H7_001663"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="ACG8H7_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP8983761.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="ACG8H7_001664"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="ACG8H7_001664"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP8983762.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4027 a         3056 c         2594 g         4835 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta ga
//
LOCUS       DAZKAS010000067        14205 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000067 DAZKAS010000000
VERSION     DAZKAS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.069"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..901)
                     /locus_tag="ACG8H7_001665"
     CDS             complement(77..901)
                     /locus_tag="ACG8H7_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP8983763.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(874..1554)
                     /locus_tag="ACG8H7_001666"
     CDS             complement(874..1554)
                     /locus_tag="ACG8H7_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFP8983764.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1577..2515)
                     /locus_tag="ACG8H7_001667"
     CDS             complement(1577..2515)
                     /locus_tag="ACG8H7_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP8983765.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2760..3611)
                     /gene="ispE"
                     /locus_tag="ACG8H7_001668"
     CDS             complement(2760..3611)
                     /gene="ispE"
                     /locus_tag="ACG8H7_001668"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP8983766.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3765..6338)
                     /gene="mprF"
                     /locus_tag="ACG8H7_001669"
     CDS             complement(3765..6338)
                     /gene="mprF"
                     /locus_tag="ACG8H7_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP8983767.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6479..7285
                     /gene="proC"
                     /locus_tag="ACG8H7_001670"
     CDS             6479..7285
                     /gene="proC"
                     /locus_tag="ACG8H7_001670"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP8983768.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7364..8920)
                     /gene="rny"
                     /locus_tag="ACG8H7_001671"
     CDS             complement(7364..8920)
                     /gene="rny"
                     /locus_tag="ACG8H7_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFP8983769.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9331..10380)
                     /gene="recA"
                     /locus_tag="ACG8H7_001672"
     CDS             complement(9331..10380)
                     /gene="recA"
                     /locus_tag="ACG8H7_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP8983770.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10489..11730)
                     /locus_tag="ACG8H7_001673"
     CDS             complement(10489..11730)
                     /locus_tag="ACG8H7_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFP8983771.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11970..12548)
                     /gene="pgsA"
                     /locus_tag="ACG8H7_001674"
     CDS             complement(11970..12548)
                     /gene="pgsA"
                     /locus_tag="ACG8H7_001674"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP8983772.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12757..13680)
                     /locus_tag="ACG8H7_001675"
     CDS             complement(12757..13680)
                     /locus_tag="ACG8H7_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFP8983773.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         4071 a         3157 c         2477 g         4500 t
ORIGIN      
        1 tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc
       61 ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga
      121 tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat
      181 ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga
      241 tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca
      301 tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag
      361 taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat
      421 cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg
      481 caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa
      541 acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg
      601 ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga
      661 tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg
      721 taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg
      781 tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc
      841 cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca
      901 ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc
      961 atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag
     1021 acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa
     1081 taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga
     1141 caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg
     1201 tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc
     1261 tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg
     1321 aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat
     1381 tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc
     1441 attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag
     1501 tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca
     1561 acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga
     1621 cagaaatata attttcgcct gctttgattt gttcattagt caagctttct aaaggattca
     1681 acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat
     1741 tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta
     1801 agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt
     1861 cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg
     1921 catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat
     1981 cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa
     2041 tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat
     2101 gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac
     2161 ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta
     2221 acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa
     2281 tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca
     2341 aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa
     2401 aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg
     2461 ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata
     2521 tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa
     2581 aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg
     2641 attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa
     2701 ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt
     2761 tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact
     2821 ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc
     2881 aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc
     2941 cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct
     3001 tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct
     3061 acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag
     3121 cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta
     3181 cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct
     3241 tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct
     3301 gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca
     3361 ccggttttga tattgtattt attttttagc aataaagcag cttgataaac attgttccgt
     3421 tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct
     3481 tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac
     3541 ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca
     3601 atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga
     3661 aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt
     3721 tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc
     3781 acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc
     3841 atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc ttcccaatga
     3901 tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa
     3961 ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta
     4021 gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac
     4081 agattggcta acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa
     4141 tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga
     4201 tctttacgga caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca
     4261 gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga
     4321 tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt
     4381 tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct
     4441 gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct
     4501 gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc
     4561 gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa gcatttgttt
     4621 cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta ataaaaataa
     4681 ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa
     4741 cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat
     4801 tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac
     4861 acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac taaaaagcgt
     4921 ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca
     4981 aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac
     5041 cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg
     5101 attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact
     5161 tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc
     5221 tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac
     5281 agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac agcacttacg
     5341 agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg
     5401 attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa
     5461 taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac agacaattga
     5521 ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac catcgtcagc
     5581 attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt
     5641 ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat
     5701 gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt ctttctcttt
     5761 agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa
     5821 tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac
     5881 catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt
     5941 tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac cacaccacca
     6001 aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt
     6061 tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac
     6121 atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt
     6181 tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga
     6241 tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc
     6301 tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta
     6361 tacttttgtg tcatacccca atgttatctt atttatttta atttttccac tttagacctg
     6421 ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat
     6481 gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa
     6541 aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca
     6601 aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca
     6661 atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact
     6721 aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat cattaataga
     6781 tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa
     6841 tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca
     6901 gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc
     6961 agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat
     7021 cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat
     7081 tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg
     7141 ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt
     7201 agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga
     7261 tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag
     7321 aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag
     7381 ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt
     7441 cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg
     7501 gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg
     7561 cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt
     7621 cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga
     7681 taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt
     7741 tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat
     7801 ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt
     7861 cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga
     7921 cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat
     7981 gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat
     8041 ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat
     8101 cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg
     8161 aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa
     8221 gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa
     8281 gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta
     8341 atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt
     8401 ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc
     8461 gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca
     8521 ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt
     8581 ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt
     8641 gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct
     8701 cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt
     8761 tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg
     8821 caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga
     8881 caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat
     8941 ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat
     9001 gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc
     9061 gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt
     9121 tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa acttttctat
     9181 taaattattc tttgtagatg aacagatatt tttattttat gtacccactc gtataattaa
     9241 ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt
     9301 cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg
     9361 tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag
     9421 agcagaaact tctgccatca tttcaggatg attagccata tagattttag cgttttcccg
     9481 tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc
     9541 tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac
     9601 atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt
     9661 acggttacca acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg
     9721 gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat tcccaaacat
     9781 cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat
     9841 cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga
     9901 gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac
     9961 tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc
    10021 tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg
    10081 cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc
    10141 tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat
    10201 gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact
    10261 tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc
    10321 atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa
    10381 tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct
    10441 tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa
    10501 aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg
    10561 atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca
    10621 tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac
    10681 actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt
    10741 gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt
    10801 ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc
    10861 taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt
    10921 ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa
    10981 atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc
    11041 ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg
    11101 tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga
    11161 ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg
    11221 ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc
    11281 gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt
    11341 acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt
    11401 catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg
    11461 tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg
    11521 tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc
    11581 ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc
    11641 agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc
    11701 tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct
    11761 gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac
    11821 agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata
    11881 tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc
    11941 aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt
    12001 ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt
    12061 tgatctactg gaataccgat caggctgaat gggatattat tgataaacag caagataata
    12121 gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca
    12181 tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct
    12241 actacccaac caggggcctt tccttggtca accaacatga taaaagcagt cataactagc
    12301 attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg
    12361 gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact
    12421 agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa
    12481 cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt
    12541 aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac
    12601 cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc
    12661 tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac
    12721 ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag
    12781 gttcacattt tttggactct gattgttttc ccctggatta actggtacgg attctccatt
    12841 cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc
    12901 cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt
    12961 gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt
    13021 ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc
    13081 cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg
    13141 cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc
    13201 tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac
    13261 gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact
    13321 tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg
    13381 acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc
    13441 tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc
    13501 aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt
    13561 aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc
    13621 tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat
    13681 ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc
    13741 cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca
    13801 ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc
    13861 tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc
    13921 ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga
    13981 tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac
    14041 caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc
    14101 agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc
    14161 gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttct
//
LOCUS       DAZKAS010000068        13810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000068 DAZKAS010000000
VERSION     DAZKAS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.070"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG8H7_001676"
     CDS             complement(221..1423)
                     /locus_tag="ACG8H7_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8983774.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG8H7_001677"
     CDS             complement(1426..1620)
                     /locus_tag="ACG8H7_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP8983775.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG8H7_001678"
     CDS             complement(1667..2515)
                     /locus_tag="ACG8H7_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFP8983776.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG8H7_001679"
     CDS             complement(2720..3070)
                     /locus_tag="ACG8H7_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983777.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG8H7_001680"
     CDS             3248..4111
                     /locus_tag="ACG8H7_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8983778.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG8H7_001681"
     CDS             4735..5727
                     /locus_tag="ACG8H7_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8983779.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG8H7_001682"
     CDS             5743..6627
                     /locus_tag="ACG8H7_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8983780.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG8H7_001683"
     CDS             6676..8283
                     /locus_tag="ACG8H7_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8983781.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG8H7_001684"
     CDS             8284..9768
                     /locus_tag="ACG8H7_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFP8983782.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG8H7_001685"
     CDS             9945..10937
                     /locus_tag="ACG8H7_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8983783.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG8H7_001686"
     CDS             10989..11858
                     /locus_tag="ACG8H7_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8983784.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8H7_001687"
     CDS             complement(12278..>13810)
                     /gene="guaA"
                     /locus_tag="ACG8H7_001687"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP8983785.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DAZKAS010000069        13787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000069 DAZKAS010000000
VERSION     DAZKAS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.071"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG8H7_001688"
     tRNA            2..74
                     /locus_tag="ACG8H7_001688"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG8H7_001689"
     tRNA            78..150
                     /locus_tag="ACG8H7_001689"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG8H7_001690"
     tRNA            155..225
                     /locus_tag="ACG8H7_001690"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG8H7_001691"
     tRNA            238..311
                     /locus_tag="ACG8H7_001691"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG8H7_001692"
     tRNA            323..394
                     /locus_tag="ACG8H7_001692"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG8H7_001693"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG8H7_001693"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP8983786.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG8H7_001694"
     CDS             1983..3455
                     /locus_tag="ACG8H7_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFP8983787.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG8H7_001695"
     CDS             3538..4326
                     /locus_tag="ACG8H7_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8983788.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG8H7_001696"
     CDS             complement(4393..5061)
                     /locus_tag="ACG8H7_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFP8983789.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG8H7_001697"
     CDS             5176..6888
                     /locus_tag="ACG8H7_001697"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983790.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ACG8H7_001698"
     CDS             6891..8660
                     /locus_tag="ACG8H7_001698"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983791.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ACG8H7_001699"
     CDS             complement(8865..9431)
                     /locus_tag="ACG8H7_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8983792.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ACG8H7_001700"
     CDS             complement(9431..10387)
                     /locus_tag="ACG8H7_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP8983793.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ACG8H7_001701"
     CDS             complement(10387..11043)
                     /locus_tag="ACG8H7_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFP8983794.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ACG8H7_001702"
     CDS             11413..13431
                     /locus_tag="ACG8H7_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP8983795.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4282 a         2490 c         2932 g         4083 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaac
//
LOCUS       DAZKAS010000070        13733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000070 DAZKAS010000000
VERSION     DAZKAS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.072"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..176)
                     /locus_tag="ACG8H7_001703"
     CDS             complement(<1..176)
                     /locus_tag="ACG8H7_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP8983796.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
     gene            complement(655..1374)
                     /locus_tag="ACG8H7_001704"
     CDS             complement(655..1374)
                     /locus_tag="ACG8H7_001704"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFP8983797.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1423..3069)
                     /locus_tag="ACG8H7_001705"
     CDS             complement(1423..3069)
                     /locus_tag="ACG8H7_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFP8983798.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3248..3838
                     /locus_tag="ACG8H7_001706"
     CDS             3248..3838
                     /locus_tag="ACG8H7_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8983799.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3930..5435
                     /locus_tag="ACG8H7_001707"
     CDS             3930..5435
                     /locus_tag="ACG8H7_001707"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFP8983800.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5519..6871)
                     /gene="celB"
                     /locus_tag="ACG8H7_001708"
     CDS             complement(5519..6871)
                     /gene="celB"
                     /locus_tag="ACG8H7_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP8983801.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6871..7389)
                     /locus_tag="ACG8H7_001709"
     CDS             complement(6871..7389)
                     /locus_tag="ACG8H7_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983802.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7405..7731)
                     /locus_tag="ACG8H7_001710"
     CDS             complement(7405..7731)
                     /locus_tag="ACG8H7_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFP8983803.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7744..9723)
                     /locus_tag="ACG8H7_001711"
     CDS             complement(7744..9723)
                     /locus_tag="ACG8H7_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8983804.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9744..10058)
                     /locus_tag="ACG8H7_001712"
     CDS             complement(9744..10058)
                     /locus_tag="ACG8H7_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFP8983805.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10226..10519
                     /locus_tag="ACG8H7_001713"
     CDS             10226..10519
                     /locus_tag="ACG8H7_001713"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFP8983806.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10597..11709)
                     /locus_tag="ACG8H7_001714"
     CDS             complement(10597..11709)
                     /locus_tag="ACG8H7_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP8983807.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11862..12710
                     /locus_tag="ACG8H7_001715"
                     /pseudo
     CDS             11862..12710
                     /locus_tag="ACG8H7_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13063..>13733)
                     /locus_tag="ACG8H7_001716"
     CDS             complement(13063..>13733)
                     /locus_tag="ACG8H7_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8983808.1"
                     /translation="LQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVA
                     IFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRK
                     KRNI"
BASE COUNT         4307 a         2788 c         2349 g         4289 t
ORIGIN      
        1 ctagccaaga acccatctgc tgaaatatga aaatttttga atgctacgac attcgccatt
       61 acaggtatgt aaaagaaaaa aatcatccaa agcatacccg gtacagccat caggacaagt
      121 gctttataac gccatagatt cataaaaaaa tgattgaacc cctttcgttt tttcatctaa
      181 aaaccccttt cgattcattc gcaacgtaat tatagcgctt tcaataatga gaataaataa
      241 acaaagtata gggaaaatga aacaaagtat aggagaggtt gtgaaatcag cgttttggcc
      301 tgagtattag agaaaaaaac gggaaaatag ttcctcatat tttgccgttt tttgagctaa
      361 taccgagggt cagctgattg aacaccgttt atcagaggtt gtgaattcag cgttttggcc
      421 tgagtattag ggagatcgtg tccttactat ctaactctga gtcacaagga acaatgggga
      481 ctgtaacaaa agtcttgtca cagtccctta ttccgaacaa acggtgttcc agaagtagct
      541 tctacggaaa taagccgaaa tttcacaaaa aagccatccg aaagtcagac ggacaagtct
      601 gcttttcaga tggcgctaca actactctgc ttattctttt agctatccat tcaattattt
      661 ttctctcact tgttctagct cttctttggt catgggtcga tattgccctt tattcaaatc
      721 tttaggcagc caaaattttc ccatacggat tctttttaaa tataagactt cttttcctac
      781 tgctttgacc attcgcttaa cttggtgaaa ctttccttca tgcaaaatca gacggataat
      841 tgaagtttct gtctcttgat ctacttgatc aatttggagt tttgccggca atgtctgctc
      901 atcgccatca ataatcagtc cttctgcaaa taaacggata tcttcttctg tcattatacc
      961 gctaacctca gctagatatt ctttctccac atgtttttta ggtgatagta actgatgggc
     1021 aaacaccccg tcattcgtta aaatcaagag gccctctgtg tctttatcta atcggccgac
     1081 tggaaaaaga tcttcccgat aatcctggtc ttccaataaa tcgattacgg tttgatcatg
     1141 attatcctga gtagcagaaa tgactcctgc aggtttatgc aaaatgtagt aaaaatcttt
     1201 ttgatacgta atgatttctc ctaaataagt aaccgcatct tcgaattcat ttacttgcgc
     1261 tttatcgctt ttcaccactt gtccattgac ttcgatcgtt ccttttttga ttaatccttt
     1321 gacttctttg cggctgccaa gtcccatatc tgctaaaaac ttatctaaac gcatgcgttt
     1381 cccccatttc aaaagagcat caatctagct cttctgattc acttatttta tttttaattt
     1441 atgtcgtaaa cgcgctattc ctggtcctag tagtctttct gccaagcgga ttttcaaagc
     1501 taaataacca tacactaaag cccctatacc tgctactaca agaatcaaca atagagactg
     1561 gaatttgcta tcttcgttta aaaaagcacc aaaaatcatc tttgtgatcc acgaagccaa
     1621 aagcataatc aaggtaagaa taaagatcag caatgtcctg cgccagacaa aacttggttt
     1681 aaagcgggct acttcatgga ttctctttaa gatcaaggta caagacacag caaatccgat
     1741 cattgtcgcc aataaagggc cgtatacttc aaataaacga atggctgggt attgaagaag
     1801 gagtttcact agaaacccaa taaccaaata attaattgcc gccttgtttt caaacattcc
     1861 ttgcagcatg ttggaaacta gcatatatag tcctaggaag agtcctacaa aacttgcttg
     1921 gatcagtaca ttacttccta gagaatccgg tgtataaaat aatgtattta gcggataggc
     1981 aagtaacata acaccaaatg tcgccggaaa cataaaaaat gaaaacaact gtaaatttcc
     2041 gcttgtcaat tttgccagac ctactcgatc tttcaacgta acagcttctg taatcaacgg
     2101 taacccagta gacgcgatcg atgttgccaa tgcaataact accatcgtca acttatctgg
     2161 attggcacta aaaatcgaaa aaagatccag taactgtgca ttggaatact cggtggatgc
     2221 agacatgatc ttcataaaag tgaactgatc gactaatttg aagatcgtta cccctgaacc
     2281 cacaataata aacggaatcg cttctttaac ggtatctaac aacaattcct tcgttgcaat
     2341 atttattttg ttttcgctgt agtgaataaa cgccgatgta tacgcttgat gtttcttcaa
     2401 aaaatacaaa aggacaagaa tacttgctag cataccgata aatgccgcga aagttgattg
     2461 cgtgaccgcc gtcacatagt caccatccat gactttcatg atgatgaatg tagccaatag
     2521 catataaaaa atacgtgcca cctgttctac aatctgcgac agcgcataag gcatcatttc
     2581 ttgattgcct tgcaaatacc ctcgtatcac actcatacaa ggaaaaacca atacagcgat
     2641 actcaaagaa cgcatcgttg gaatcagttc ttctccgcca ccagaagcat gagctagcca
     2701 aggagaggcg atgtacatga acaaagcaaa caacaggcct aacccgccca tcatctgtaa
     2761 tgcacggata aataatcgtc gtgaagtgcc atattcattc aatgaattat agcgtgcagt
     2821 ttgttttgca atcgcagcag gtattcctgc tgtagagatc agtagaaaca atgcatatat
     2881 attgtatccc atattgaata atccattggc agccttagca ttttcgccca tccaagcata
     2941 ccagggaatg atatagacag cgcctagcaa tcttgaaatg atattactgg cggtcatcca
     3001 agcagagcct cgtgccattt tttcttgcgg tgttaattga tctaatgctg gttttctttg
     3061 attatccatc ggtcagattc caactttcta caaaacatta ctatcatttt aattggtaaa
     3121 acagaaactg gcaatcgatt ttatataact tttataagaa caattgtttt tttgaattcg
     3181 gtttcgtttt tttggtaaag taaagaaata tacagccttt tatggctaac atttatagga
     3241 gatatcaatg acacaagaaa taaaaaaact acccgttata tttttaggtc ttttctttat
     3301 cacagttgga ttgaattggt tcttgctccc tcacgatata gcttccgccg gagtgggcgc
     3361 catcggacat ttagttgaaa cggccttttc tatccaacgc tcagttactg tttggagcat
     3421 caatcttatg atgctgcttc tagccgctgt cctattagga aaatcagtat tttttaagac
     3481 tgtagcggga agtctgcttt ttcctgttgt tttagagatt gttccaaaag tggaaatgat
     3541 ctcctcacat ttcttttcac tgatttcagg aagtcttttg ttcagtcttg gggtttacac
     3601 attgtacaca gtcggggcct caaatggcgg tataacgata ccgccgatta ttttccacaa
     3661 attttttcgg cttccaatgc cgcaaggact tttacttacc aatagtctta tcgtcttttt
     3721 gaattattat gtttttggat tgttggagac actattcgtt ctcctatcta ttacagtcag
     3781 ttcattgttt atgaaaatac tcatccgctt gtcccctgtg tcaagaaaat ttgaataaag
     3841 ctggattttg tcatagcgga aacaaacgat atattaaaaa gagtgtgcta taataaacca
     3901 agaaattgaa taaagcgagg gatcaatcaa tgagcttctc tttagaagac atccgtcatt
     3961 tactattaaa agaaaatcta ttaaaagaat ttgtttcaac aagaggctgg catcttgatg
     4021 taccagaaaa agctgaattt cttcagttaa gttatgattc aagaaaagct gatgcttcta
     4081 ctttgttttt ctgtaaagga atgaatttca aagaagaata tttggattca gcaatcgaac
     4141 agggtattca atattatgta tcagaacaac catacgaaaa ttcagcagta ggaatcatcg
     4201 taacggatat ccgtaaagca atggcgcttc ttgctatggc tttttacgac tatccgcaaa
     4261 ataaattgaa agtgatcgga ttcactggaa caaaaggtaa aacaactgct gcgtacttta
     4321 caaaagcaat cttggatcac acaacaaata aaaaaacagc attgctttct actatgaata
     4381 caacgttaga tggcaaaaca tatttcaaat cccatttgac aacgcctgag tcattagatt
     4441 tatatcgtat gatgtcagaa gcagttgaaa atggtatgac tcatctagtg atggaagttt
     4501 cttcacaagc ttacaaaaca caacgcgtat acggcttgac tttagatgtc ggaatcttct
     4561 tgaatatctc gcctgaccat atcagtccga tcgaacatcc gacatttgat gactatttct
     4621 attgcaagcg acaattgatc ttgaattcaa aaacagttgt tttaaatcat gaaagtgact
     4681 attttgattt acttagggaa acagccgatc tattccagat tccaacgatc acttatggtc
     4741 gttcggaaga aagcaattat caagtgatcc gttcagataa aggtactcac ggttttactt
     4801 tatcttctca tgaagatcgc ttagctatct gcgatactgc ttatgatatt ttgctggctg
     4861 gaggattcaa ccaagaaaat gcagccagtg cgatcattgc cgcagcacta acaggtgcct
     4921 ccagtcaaga tgcacaagac ggattaaaag aagctcgtgt accaggccga atggatcaat
     4981 tgatccaatc caatggagcg catgtctatg tggactatgc acataattat ctaagcttga
     5041 aaacattgct gaaatttgcc aaaaatgaac atccagatgg ccgtgtaatt gttgtattag
     5101 gcagcccagg aaacaaagca atctcccgtc gacatgattt tggaaaagtg ctatcagaaa
     5161 cagctgacat cgcctttttg acggcagatg acccagcatt tgaagatccg aaaaaaatcg
     5221 cggaagaaat caatgaagca attactaatc cagatttgat cgttcattat gaaatggatc
     5281 gtcctgaagc gattcgacgc gctttagcag aaagcgctcc gcaagattca gtcgttattg
     5341 cgggaaaagg cgtggatccc tatcaaaaaa tcaatggtgt agatgaacct tatgaagggg
     5401 attatgcaat cgtgaagcgg ttgattgaag aataatggta tttatatact agtggtttcc
     5461 catttctaag aaaaaaaggg actgtgattt tttgtcacag ttcctttttt cttagaaatc
     5521 attttaactt ttcactagct aattctgctg ccttttctct ttcttcattt aataatttat
     5581 tatcatagaa tttgataaat gggaaccaga taatgaatgc gatgattgca caaatgactg
     5641 ctaacacagc agctttccaa tcaccaccac taccaacaaa tgctccgatc ccgactggtg
     5701 aaggccacgg catctgtgca agcatcggtt tgacgatttc cagccgaatg gcaaaataag
     5761 cgagtgtagc agaagccatc ggagctaaga aaaacggaac cgctaaatag ggattgtaga
     5821 cgattggcat cccgaaaatg attggttcat tgatattgaa taatcctggt acaagtgagg
     5881 cttttcctaa cactttcagc tgatcagatt tcgcgaggaa tgcaataaag atgatcaatc
     5941 ctaaagtcgc acctgatcca ccaacggtta cataagaatt gttgaattct cctgccaacg
     6001 gataatttgc gccttgtgcg ttagctgcca tattagctaa tacgatcggg gtcaagaaag
     6061 aagtgatgat attcgctcca tggatcccta cgatccacaa tgcatggatc aagaaataaa
     6121 tcaccatgac accaagccat gtattcgtca actgcgtaac aaacccaaat gggatagcaa
     6181 tcattttgaa gatatccgtt cctagtgcaa ctaataatcc attgatgatc atcaccacaa
     6241 acgcaatgac aaatgttggg atcagagcag tgaaagaacg agaaactcct ggcggaacaa
     6301 catctggcat ttttatgacc caatttctct tgacacacat acaatacaat tcgaccgcaa
     6361 taatcgacat aataatagct gtaaagattc ctgaagttcc taaacgttcg acaaaagagc
     6421 ccatcctaat accatttact acagtttctg tctcattgat agaagcaatc aagctgattt
     6481 taccatcagt catgatcaat tcagggatac acataaagaa tgcaaaaaca gatagtaatg
     6541 cccccgtgag cggattaaca tttaattttt gttcatctcg ttcaattgac gttaattcat
     6601 aagcaaacac aatggcaaag taaatagata aaatccccat cgtcattgta ttgacgatca
     6661 tgtacaagtc actaactttg aaaaaagttg cattaaaaaa tccttctaaa aatgggaacg
     6721 gtaatggcaa aatatttaaa accaaaaaca tcgagcctac aatcgtgaaa ggaatagaag
     6781 ccatccccgc tgccatgaca gcccgaacga ttttaagttg cgcaattttc cccataggtc
     6841 ccatcaggta tttctgcaaa aaatcaaaca ttagttattc tcctttccta aatataacgc
     6901 tttttcatat tgtttaatac ggatatactg ttggtcttgg ttgagtgaaa ttttctttaa
     6961 acgatactgt ataaatgcac tggcaaaaat accgcttaca acaagaatga aaacaaataa
     7021 ctgtcctcca ttgttatttg ccataaaagg atacaagaat gtaaatcctg aggtaaacaa
     7081 cactggaatc actaataaat taaagacact ctggaacaaa aaataccact ttgtccatgg
     7141 aacgttcgtt tggtgttttc gataaagctt tacttgttct cctactgcag gcaaaatagc
     7201 taaaatcaga atcatcggta aactacttcc taaactttta gctgatagaa gcagaatcat
     7261 ccattgcatg ttcatgaaaa agaaaaaggc agtcaggtaa cgtacgatta gataacgatt
     7321 gaaatacatg tttttcaaac taagttgctt tttttctaat tcgattttcc tttttactgt
     7381 atcctgcaca atgatccttc ctttctattt atttttttga tacaaataat tcatttcctg
     7441 tgcaacttcc aataatgtca tcgtcgtcat cagatgatct tgtgcatgga ccatgataat
     7501 ttccatttct atttttgtcc cgctggcata ttctttcaga agttgagttt gtgactgatg
     7561 cgcatgaaga atctcttcat ttgcttgtcg caacttctct tctacttctt caaacatctc
     7621 tttacgcatc gagcgaaatg cttcatgtat cagtgtccgt gcatttccgc tatggaaaat
     7681 gatatcaaaa gaagtcactt gtagtgtttc actgtttatt ttttcagcca tctgttttcc
     7741 tccttattcg atcagtttga taaatttact gctgaaattc tcaaaaatag gttcagctaa
     7801 gatttcctgc tgtactttta gctggtcaac taacctaacg attgctttag tcacgaccgt
     7861 aattccttca ttttcgacat aagaaggcga aactaaaaaa acaaaatgaa tttcttgata
     7921 ttgatcccac tgcatcccag aaggaatcaa tgcaacggct acctttgttt cgattccaac
     7981 tggaacagcg ggatgtggga ctgccactgt atcactgaat atgatttgtc ccatattttc
     8041 acgttgctgg atctgctttc gcatttctct tatatatcct tcttcttcat ttatgctcag
     8101 cttttgcagc aaatcatgaa gaatttcttc ctttttcggt tcgtacgtat aagtcttgaa
     8161 aaatttttca gacaactgtt cggtgtaaat ctgctttttt tgctgtagag aaaatgctgg
     8221 tatcggtcta tctgaggaat gatagggtat agattctcca actacctttc gtattttacg
     8281 aacatcatct tcattcaaaa aaatactgac atgtaaaact ggaattttga aaaacattgt
     8341 cgaaagatca atagaagaaa taatcaggtc gattcctttt aaagttgttt cattgatctc
     8401 ataatagcct tttacactta ccactgtaat atttttaccg aattcactga ctacgcgatt
     8461 ctttaatagt tgggcactgc cgtaacccgt tgcacagata atcaatgcat gtagtttatg
     8521 cgcagctctt tctttttcca atgccgccat gagatgcaaa gccaaatagg cccattcatc
     8581 ttcattgatg gtatagtttt tcaaagccgg catgttgctg aaatgccttt ttactgcgtc
     8641 aaagactttg ggattttctt tctttatctc ctcagtcaat gggttctcaa tgcgaatacc
     8701 tcggctcaat cgaatcatca tcggtgacaa atgatcgagt agtccattga aaagctggag
     8761 atcctctact aatgaaaaat gaaattcctg agacaatctt ttcagaactt tcgctaattc
     8821 ctttgatagt tcctgatcat tttgattttc tttgtgattc gacttagcca tgagatgtaa
     8881 tgtcaaataa gcaatttctt cttcgggaaa cgatacacct gcgattacct caatacgttg
     8941 aacgatccgc tgcgccactt gatactctac tcgcttagaa acttcttgtt cgatgcctaa
     9001 ttccttgatt tcaaaccctt ctcggagccg tttgatcccc aaagccaagt gtaaagtcag
     9061 attctgtatg ataaaatccg aaacttttaa cttagcttct cttgtttcat cgagaatgat
     9121 aatgactagc tcttcaaaac tgatgtctgg gaaaaaacgg ctttgcccta aatattcttt
     9181 caatgaattt ccataatggt ttccaaaaaa agtatccatg ataaaatgcc gtcgatttct
     9241 ttcttcccct tcgatccatg tcccttgacc atgcttagaa acgattttta gtgaatatgg
     9301 aatcaatcgc tccttgattt cttgcatgtc ctttgtcaaa ctggaacgac tgatataaag
     9361 ctcttctgcc aaatcatcca taaaaagaat ggcatcttcc aaaagcaatt ggttcaatat
     9421 atacttttgt cgatcttcgg actcataaaa tccttgcggt tctctagcat atccaggtac
     9481 tatttcttgc cgactcaaaa acaagtcaaa tgtcagtctt tgtaaaatat gcagttggta
     9541 gccataacct ggtttagcaa tgatcttacc tccgtttttt tctactaact ctttgatccg
     9601 ctgcaagtaa ttacggattg tccgatcaga taataacaat tcagaagcta aacgttgact
     9661 ggtgacatac tccttcttgt gatcaatcaa atatctcaac agcaattttt ccttctgttt
     9721 cattatcatc cactcctatt ccatcatatg ttctcttcga tcacctctgc taacttctgg
     9781 atgcctgtag ggattgggac atacgcttgg aaagggatac taacaacagg ctttccaaca
     9841 gattctccta attttttgaa tttatccaaa aacatcgtcg tttgcgggct gatcagaaat
     9901 aaatcaaaat cactattctt aatcatatta tcgccttctg tagctgaaac tgcatctacg
     9961 agtacctctt ctcttttttg cttaaagaaa tccgttgtct ttgaggccat catcgaggat
    10021 gacattcctg ccgcacaaat aatcaatgct tttttcataa atcaatcact ccttttattt
    10081 ttataaacca agcatatctt gtaagcgttt tctattcaaa atatatattt ccgtagcatt
    10141 gcggaaaaaa tgtgtatcat atcttgaata ataatttctg gcaccgtgat atactagtga
    10201 tagaattttt tcaggagggg atttagtgag tatcacagtg aatattttgt atacaagtac
    10261 aaatggacaa gccagaaaat ttgcagaaga aatggtcgaa agaaacattg tcaaagcgat
    10321 tcgtgcagaa gaaggaaacg agaaatatga ctattacttt ccactagaag atccagaatc
    10381 tcttttactg atcgaccgtt ggaaagatga agaagcaatc gaaaagcatc acaaatccga
    10441 aatgatggct caaataaacg aactccgtaa aaagtataaa ttaaaaatga aagtcgaacg
    10501 tttcactgat ttaccgtaaa aagatacaca tggaatgata gcaatttatc atccatgtgt
    10561 atcttcactt tttccttatt ttttattcga tctcatctaa tgattcaaac cgtgtttttc
    10621 tcttattttt cggataagta tttcagtcac cccaatatac atgattccta aacatacccc
    10681 aaggatattc tcaaaaatgc ggatcgttac tgctcctgga accccgaata aactggcagt
    10741 gattgccaaa gcaccaaaag aattaaaaac tgttttatat ctataggtag aacaaacacc
    10801 taaagctaga ccacctaata taccgaccca gttaaaagga ataaattggc tgataccaat
    10861 aaataaaaca catccaatca atgagccaat gattcggtct actgctcgtt ctttgaaacc
    10921 aatcgacatc aatccatagc tagatacaat agaagaaaat gcaaaccctg cccacatcaa
    10981 tcgctgaaaa gggacatact ctcctgcaac gatcaaaaga ctaatgccaa atgcatagat
    11041 gagcatccaa attttttcct gcgaatacat gcctttcaaa aaaatctctt taaacaagct
    11101 tttgtctttg ttcttttctc gatgttttct gtaaaggaga actgaaaacc aacaaaaaaa
    11161 caaaaataat aagctacttg tttgtgtaaa gaaattcttg ttcaacgaat ctttaggtaa
    11221 cgaataaaca ataaagagat atgaaaagcc gtacaaagaa gcattcccca ttttgggatc
    11281 actagcggtt gccattaata tgctgcttag taaaataaaa tgaatcaaca tgcccataac
    11341 tgaccatttg atcagctgga catacggtgc tactaatagt gagaacatga cgattgctaa
    11401 acctatgatc gaatgggaaa tcttataacc aaagtcaaca aaacgaatac tcatcaacat
    11461 acagaataaa accacagcgt atggtttatg cttcgattca aataatgttg taattataat
    11521 gataaaacaa atggcaaaca atacgatgaa taatgatcgt aaaaacaatg aacaaatata
    11581 catgcacttt tcttttttag aagctgcttc tcgtattttt tgttttaaaa taaacggatc
    11641 tagttgtaga gactgataaa aatccacctg ttgtcgtcct ttctttttac ttttattttt
    11701 tacagtcatc tatgacaggt tgtaaatact atggaacatc atatggcgtt gtcattttct
    11761 tgtcaactat ttttcctatt catgttttta taaaaaaaca tatcgttttc ttgtatgatt
    11821 aaatcaccac aaaataaaca actgagagaa cgatatgctt tatgaatggt tctatcaaaa
    11881 aaattctgag atcaatagac aaaaatttga tgattacaga ggtctcttac gaaaccttac
    11941 agaagaaaaa aacattgatc gttgctgcag ttctcttccc tgctccatgt gtctgtagaa
    12001 attgtggttc tgctgtggta gatggaaata ggaaattaat ggtggtgaaa aacaagaaga
    12061 aggaaaccat tgtccacttt gaataatata atcatatgcc aatggttata cgcctaaaaa
    12121 agcagcgcta tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt tattttatcc
    12181 gtccaaggca ctcgatttca aatcatgtca gacataaaat cacttcttta ctgactgaaa
    12241 aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc tttgacaacc gttattcgca
    12301 cattgaaaga gttgagaagc tatttaccaa agtcttccaa aagaattctg tcttgagtgt
    12361 tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt ttatttccgc
    12421 ggatggcgag acagaaaaat taatcgagaa aatttaccta cacaaaaaat tcctcgatta
    12481 acaaactatt tccttagctg cactaatcct aaagaaatag aattcttagt tacagacatg
    12541 aacgccacct actttcagct caccaaacat gttctaccaa atgcgaaatt ggtgattgat
    12601 cgattacttg gtttttccac gcctttcaag gaggcgtaac ctttattcac gagttggttg
    12661 aagctttccg agacaaaaga cccgacttgt ttttctcctt attgacaaag agccattaaa
    12721 actagagaaa aaattacaac tgatcgctga ttatttgtag aaagaaaatc aaagtattac
    12781 ccaataaact tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga ttttcgcagt
    12841 gtttttgtat attttttatt aactagtatt actacaaagg tttatttttc acttctcaac
    12901 gcagcacaaa attgtcttta cttatttact ggttgggttc aaccttctct actccgatac
    12961 taatatttgt ttgtataccc cttctaaaat cggtagtttc cattcaattg atgggtacta
    13021 gttgatttta aaaactatgc ttattcaatt ttttatttta gctcatatat ttcgtttctt
    13081 tctaaatttt atactgatga aaacaagtag cacacccaat attgacaata taaactggct
    13141 aatgatggtt tctccagttt ttggaagatg cttatttcca ctgcttgttg gattgtttcc
    13201 tcttatatct gagctatcag caacttttct attctctttt gttaaaaaga tcgccacttc
    13261 ttgttctttt tcgatcttga aagttactgg tgtcgcatcc aattcatacc ccgttggcgc
    13321 ttgagtttct accagctgat atgctcctgg tgctaagcca tctatcgcca gttttccatc
    13381 tgctcctgtg gttagacctg actgcagaac ggttccattg gcatcttgta actcaaaaac
    13441 agctccttgc aggacttctc cgctttggtc atcgatcttt gtcaacacga caccgcctgg
    13501 tgtaagtcga ttttctttcg tcaattctac caccgctgtc tgacttcttt cgatctcaaa
    13561 ctcgattggt gtcgcatcca attcataccc cgttggcgct tgggtttcta ccagctgata
    13621 cactcctggt gctaagccgt caatcgccag ttttccatct gctcctgtgg ttagacctga
    13681 ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca gctccttgca gga
//
LOCUS       DAZKAS010000071        13687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000071 DAZKAS010000000
VERSION     DAZKAS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.073"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..506
                     /locus_tag="ACG8H7_001717"
                     /pseudo
     CDS             192..506
                     /locus_tag="ACG8H7_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(811..1371)
                     /locus_tag="ACG8H7_001718"
     CDS             complement(811..1371)
                     /locus_tag="ACG8H7_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8983809.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1513..2127
                     /locus_tag="ACG8H7_001719"
     CDS             1513..2127
                     /locus_tag="ACG8H7_001719"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8983810.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            2316..4196
                     /locus_tag="ACG8H7_001720"
     CDS             2316..4196
                     /locus_tag="ACG8H7_001720"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8983811.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            4275..4502
                     /locus_tag="ACG8H7_001721"
     CDS             4275..4502
                     /locus_tag="ACG8H7_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFP8983812.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4507..5049
                     /locus_tag="ACG8H7_001722"
     CDS             4507..5049
                     /locus_tag="ACG8H7_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP8983813.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            5276..5992
                     /locus_tag="ACG8H7_001723"
     CDS             5276..5992
                     /locus_tag="ACG8H7_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFP8983814.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(6052..7887)
                     /locus_tag="ACG8H7_001724"
     CDS             complement(6052..7887)
                     /locus_tag="ACG8H7_001724"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFP8983815.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8083..9081)
                     /locus_tag="ACG8H7_001725"
     CDS             complement(8083..9081)
                     /locus_tag="ACG8H7_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983816.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9300..10520
                     /gene="sstT"
                     /locus_tag="ACG8H7_001726"
     CDS             9300..10520
                     /gene="sstT"
                     /locus_tag="ACG8H7_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP8983817.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            10667..11302
                     /locus_tag="ACG8H7_001727"
     CDS             10667..11302
                     /locus_tag="ACG8H7_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFP8983818.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            11493..12233
                     /gene="truA"
                     /locus_tag="ACG8H7_001728"
     CDS             11493..12233
                     /gene="truA"
                     /locus_tag="ACG8H7_001728"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP8983819.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(12378..>13687)
                     /locus_tag="ACG8H7_001729"
     CDS             complement(12378..>13687)
                     /locus_tag="ACG8H7_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983820.1"
                     /translation="YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALT
                     FLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQ
                     KIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNN
                     NYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVK
                     SLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHA
                     NKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSN
                     KLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVI
                     TYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4585 a         2160 c         2673 g         4269 t
ORIGIN      
        1 aaactttata ttataataat gaagcttaat cttctttata gagaattggg ctatttttaa
       61 tggcaaagag gggaagaaga tgtttcaaaa caaaggacaa cgctatgttg taaaaggagt
      121 aaaggaatct tactggaatc aaaaatcttt attgagaaaa ttatctttga atttaagcac
      181 aaagaaatga aggtacacta gatagcaatg aagatatctt tgtcattaaa cgtcgctatt
      241 ttacacaagt aagtgatgat ttgattcatg tcttttccaa tgagtggttt gtttctgaga
      301 aaaaattaca tgcttctaat ctgcaatata tgccaggtga agatccaatt cctaatatga
      361 aagcaattat caacagtaag gattacgaag gatataaagc caaacatcca gaagcgaaac
      421 catttaaata cccacaagaa atgaagcgag catggcgtaa aatgttagat gatgagctga
      481 ttccccttga aaatgaattg aggtaaacga gaagaattca ataaactgga tgataaaaat
      541 ataatatgac tataaattgg ttacagcttc aacctataag ttgtaataaa ttagcctaaa
      601 agtaatttat taatgatgag gtgaatttga ttggtacaac aagatatatc acttagagag
      661 ggagaagcag cactacaaac tacttttcaa taagtaggaa ttatagaaat tttactcaaa
      721 aaatcttcag taaacaattt ggctaaataa gattcagttg ttatacgaga aggttacggt
      781 tcttcttaac taagcttagt atgtttttat tcaaggcatg attttctcta tgtgatcatt
      841 cagtttctgt aagagttcta ataattcttt tttggagtac ttgtaattgc tttctaccca
      901 ccgattgaga acggatagac aaccttggac gtggtacgta gtaaagaatc tcatttcttc
      961 tgtgataatc gttttcttgt cttcatatag ccatatgttc agatagtttt cttcaataac
     1021 ttgtttaact ttcttctgaa acgaaatatc accatggttg aggataaata agcgatataa
     1081 aaaagtattt gcgtaaacat agtcaatcaa gcgagtgtat agttcctcat agctatgggc
     1141 tggatcataa gagagaattt catttagatc ccgaaaaaag ttgctttcta attgatcata
     1201 tagatcatag atatccttat aatgtgtata aaaagtcgaa cgatgtacat ctgccgtttc
     1261 tgttagttcg cgtatagtga ttgcttgaat ctttttattt actaataatg tggatagtgt
     1321 ttcagccaaa gctttttctg tttttttgac gcgtctatct gtctttttca tttacctatc
     1381 tccttatccg acatatcaaa gcaaagctgt cgcttaatcg acaaacttat agatgattga
     1441 ttatagatag tagacactgt gtctattata atgtaagtat aacacaatca ttgaagttga
     1501 ggaggcgaaa tgatgaaact aacttttaaa cccttagaaa agaaagatta caaaaaagct
     1561 atctattttg ctgttgaagg aatgaacttt gagagatata tggaaaatga acttctgctg
     1621 aatctttacg gaagatattt tttgtatatg gagttgaatc gagcaactca aattcttgct
     1681 ttgtatgcag aggacgagtt ggcaggtgtt ttgctggcgg agataaaagg agaaaaaaag
     1741 aaacatcgtt cttttggaca gcaaatatat gttaagatat tcgatttttt acaaaatata
     1801 tttgctaaag aaagtaaagg agcttacgag acagcaaatg aacaaatgct tgcagcatat
     1861 ttaaagaagc attcgccaga tggagagatc atttttttag ctgcaaatcc tgatttgaaa
     1921 acaaaaggca tcggtaccaa gttgctaaca gaatttgaaa aaagagagaa aggaaaagaa
     1981 atattccttt ttacagatag tggctgtacg tatcaattct atgaacgacg tggatttgaa
     2041 agactaaagg aaaaaattag caagataaaa atcttagata aagaaataga tctccgatgt
     2101 atgctgtaca ggaaaatcat tcaatgaaaa tttcgataga aacgacaata gttggcaaga
     2161 tcacttttag tgagtcttct tgctaactat tttttgtagt gtattctacc aaaagaaaac
     2221 cgcctttttg actaaacttg atagtggtca attttttatg atggataagt atctatactt
     2281 agtctcgtaa gaatgaaagg aaaagaggga gaaagatgaa attacaaaag tggatccaag
     2341 agcatcgaaa tcatatcacc gccttatcag ctgtgttgat tatttttggt tttatcggaa
     2401 aatttactgg ttttgtcttg ttctataaca ttgctctagc catagcttct attattgcgg
     2461 tcatacctat tatgatccat gcgtatcagg cagtgaaagt caaagttatc tcaattgatt
     2521 tactcgttac tattgctgtg cttggtgcct ttatgatcgg agaatacaag gagtcagcta
     2581 tcgttacatt cttgtttttg tttggtagct atctggaaca aaaaacattg accaaaacgc
     2641 gggagtcaat caaggctttg accgagatgg ccccatctac tgctagtgtt gtccaagaag
     2701 atggaacgac tgaagaagtg gaaatcgatg acgtggaaga aggcgatatc gtcttagtaa
     2761 aaactggtgg agctattcct gtggacggac gagtcattga cgggaaaggg tatgcggatg
     2821 aagccgcagt gactggtgaa tcacgacaag tcgtcaaaat cagtggagat gaagtcttct
     2881 ctggaacgat tttagcagat ggctacgtga cgattgaagc aactaaggtc ggagatgata
     2941 cgacctttgc caaaattatc gaattagtag aagaagcaca agataccaaa tcccatgcgg
     3001 aaaaattcat tgatcgattt tctcagtatt atactccaac cgttttactt attgcgttgg
     3061 ttgttggtct gattagtcgt gattttcgtt tagcaatcac tgttttggtt cttggttgtc
     3121 ctggcgcttt agtcattggt gcgcctgttt cgaatgtagc aggaatcggt aatggagcga
     3181 aacggggcat cttagtcaaa ggcggtgaag caatggatac tttttccaaa atcgatactt
     3241 ttgtctttga taaaactggt acattgacca aaggaactac tgctgtatcc actatcaaga
     3301 actatacaga ggatgaaggg gacagcttag ctttagtggc tcgattagaa acgttatccg
     3361 atcatcctct ggggcgcgcc gtgatccatt atgctcagga gaaagacatt gattttcaac
     3421 aattagcagt caaagataat cagacaatca agggacaagg aatcacagct gagattgatg
     3481 gtcatacagt ttgtgcgggc aacaccaaat tgattgccgc tcacaaactt tctctcacac
     3541 caaagcaagt acaagattta catcaattac aacaaacagg cagctcggtc attattgtag
     3601 caatcgacaa aaaaatcact caaatcatag gtgtgtcaga tgtgattcga ccagaagtcg
     3661 ctgaacaatt agctaagttg aaacaagcag gagcaaaaca gttagtgatg ctgacaggtg
     3721 ataatcagat gactgctgat tatgtagctg atatgttagg cattgatgaa gtccatgctg
     3781 aattattacc agacgaaaag gttcaatttg tgaaaaaata ccaagaacaa ggattgcgcg
     3841 ttgcttttat tggagatgga atcaatgatt ctccctcttt ggctgcggct gatatcggga
     3901 ttgctatggg atcaggaacg gatgttgcta tcgaaacgag tgacgttgtc ttgatgcaat
     3961 ctaattttga atcgttggtt catgcctacc gcttggctaa gaaaactgtt ttaaataccc
     4021 gtgaaaatgt agtgattgcc attggtgtcg tcttattttt actcaacggc ttatttgccg
     4081 ggtttattta catggctagt gggatgttcg ttcacgaagc tagtatcttg atcgttatct
     4141 ttaacgcgat gcgtttattg tattttggac acgaaagcaa agaagaaaaa tcgtaaaact
     4201 tgataagaat caattttttt atggaagctg taatctagaa taagactatc aagaaaaagg
     4261 aaggaagaac tacgatgaaa aaaattatta tgcaattaga tacactagct tgtccaagct
     4321 gtatgcaaaa aatcgaacat gcggtttctc aacagcctgg ggtaagcaat acgaaagttt
     4381 tgtttaatgc gagcaaaatc aaagcaaact ttgatccaga tgaagtccac cctgaacaat
     4441 tagctgcagt tgtggaaaaa ttaggttatg aagtccaatc agcaaaagta aaagaggcgt
     4501 aaatcaatga atatagtgga agaaaaattt gttcaagaaa caaaacaagc agatatcgac
     4561 catcataaac caactgctgg cgccatggtg gggcatgtat tagcaaattt taaattagaa
     4621 gaaatgaaat tgatccaagc acaattgtat gttttgggta catcttcgca agaattaagt
     4681 accctttttt cagaatatgc tgatcaagaa gcgagatggt tcaataaaat ctctcatgag
     4741 ttagtgattg aaaatgaaat cgttccgtct actttagaag aagtcatgcg ttatggaaaa
     4801 atttacgaac atggtcaaaa caaatattta ccagcctcaa caatgttgga aaactttgca
     4861 aaagattttg atttccaaaa tgtctttatc acacgggcaa tcaaattagc tgaaaaagaa
     4921 gaaaaatatg gcttgcaaca attgcttgtt gaacttttaa gtttcaataa agaaatcatc
     4981 taccaagtac aaagactcca aggaaaaact gtccgacaag atttggatga ggaagacgat
     5041 gatgactaaa agtctatgtt tcttcaaata caaatataaa gcgtgaggta aattggaaaa
     5101 tcacgaagat taaaataaaa gaagcacccg ctgatgtatt acagacaaca tcagcgggtg
     5161 cttctcagtt tttagaagat ttgaaagatt gatgagaaaa tacgaaaaat caataaaatc
     5221 acattgctta tttgatagaa taaagaagtg ggagtaatcg aaaggagttt tttaattgaa
     5281 aaaaaggatt ttagtaacaa ttggtctagc ttttttgtta gctggttgta ccaacgggaa
     5341 tgaatcgaaa aaagaatcaa caaaagaaac agcgaaagca acagttgcaa ctactacaac
     5401 aactagcaca gagagcaagt caactgcagc aagtacagaa acagcagata cctctacttc
     5461 gtcagagtct tccaaaacga ctgaaacaac tcaaacatct acttcaagtt cttctgtaga
     5521 ggcttcatct tcctcttcag aaagcagcga acaagttgaa caaacactgt ggaatgcaga
     5581 caaatccgca caattagagg catttatgac caaatggggc caaacaatgg gacaacaata
     5641 caaaagttat acgaatcaaa tgagtgtgga cttatatggt cttaaagtcc ctcaagctat
     5701 tttgaacggg gagtggaaga tggctattgg tggagtgcct gtttctgcag aatggtcaga
     5761 aagtgggaca ggtcaagcag actatcaaat cacagccgtc tattccgatg cagagacaga
     5821 accatatctg aaaaagcacg tctatctatt cgggtttcag caaaatcagc caaaagtatt
     5881 ggtgacacaa caaaatcagg ggaatcccga taattatctt tacttcaacg agacagcgaa
     5941 taatgaattg aaaaatggct ttaatcaaat cgtttatgga caagcaaatt aatgagataa
     6001 acaaggggtt gtgacatgtc ttttatcaca accccttgct cagtttattt gttagtcttt
     6061 tattttttgt gtcgcccatg ttgcactgta ctggttacct gcaacaagat catattgtat
     6121 cagacgataa tcttctagaa tttctttttg tttctcagtt aaattttttt cttggataat
     6181 ttgactatcc tctgtaacaa agacctgttt tccattaaaa cgattagaca agctttctgt
     6241 aggatcaatt gttaatgcag gtagatgatt agatacttct gtcaacaacg catagtaagg
     6301 agttactttg atattcgcat gctcaagagc catagcacca aagttattag gagcaacaac
     6361 ttctttattt aacgtagctg cttgttcacg actatacttg ttgttataaa tgaaataatc
     6421 tgtctggtgc tgttctaatc catatttttt cgaatcattc ccactataga ttgcgggtaa
     6481 atggtcacca taaaaaacaa aggtaatcgg tttatcgatt ttgtcgattt cttggatgaa
     6541 tttttttact gctgtatcgg tatagtgaag gccttgcata taagtttgta attcttttct
     6601 tctattagga atgacagcag ttccttcagc agtgtagttc aattcactat agtaatcatc
     6661 ataaggcata tggttttgca tagtggataa ttggatgaat tgtgacgttt ccgtattgct
     6721 ttgtaaagct ttcaaggttt ctttgtatgc tgaatcatcc gatatgcgtg ggctttgttc
     6781 tattttatcc gtataagtta atttatccgg actgtctaaa taatagaacc tgtcaaaacc
     6841 aaatttatca aacacttgtt ttctgcgata aagagcagct gtaaaaggat ggatggctat
     6901 tttttcatcg aatagatcgg taatatttgg acttattttc tgacgctcca ctagctgggt
     6961 ataaggtgta actaacgaag cagacaggtt actgatactc aagcctgtca gtccttccca
     7021 ttccatgtta gctgttccgc caccataacc gacggataac atcaatcctc ctgttccttc
     7081 tgttataatg cttttgatgt aaggaatagg gtctgtaggt actgtcagat tagggacacg
     7141 tgcaggatca ctgaagcttt cacttaaatt gagaacgatc gtctgattgt ttaaccagtc
     7201 acttcttgtt tggttgattt catctgcctt tgtgttgtac tttttcatta ttccattaat
     7261 tttttctttc gaatagccag caggttcttc catgatttta atgtcgatat tgtttaggaa
     7321 ttgaatgact ggtccgttat cttttacggc gccttgctgg ttaaagaaag tcttgtttac
     7381 tttaaataaa ttaaacagca agaaagaagg cgaatttttg tgattaatga aaaagacacc
     7441 cgaaaaacat cctaataata cagcgatact gataaatcga cgtttccaat gaatcttcaa
     7501 ggaatactgt ttttgtagtc tacgttgtac aataatacta gtaatcaata ataggagaag
     7561 gatcaatccg cccacgatta ataagactgg gctaaccatc gataatagtt ctgacaatcc
     7621 ggtcagcatt tttaaatctg aaggaaagac aggctcttcc cgcatttcca ttttgattac
     7681 tgtcgaaata gtgagcagta agttaatgat caaggtagca gtcaaggcgt accaaaaacg
     7741 attggaaatc aacaatagta agaagaaaaa tcccataata atgatgattg tcaggatcat
     7801 gggtgcttgt cgctgagtaa atatttttaa ggcaacctgg atagtttctt tccaaccttc
     7861 ttttggttct aaaacgaaaa tctgcacgaa ggtaaagcag taaaaaaatg ctgcaactaa
     7921 aaaataactt ttatgaacaa aaagccaatg aattcttttt ttccaacgct gagttaattg
     7981 ttcaatcgaa tcaaaagcga agtattcgat gaaacgatga atgaaacgga ttttttgtat
     8041 ataaaataaa aagataacta ggagggacac tgctaataaa tatcaaataa aaaactgccc
     8101 gatatttatc agccatgcct tccctgagtt tggaaattca cgtttcataa atgtagatac
     8161 gcgataagaa actaaaggga aattacatat aacttgagtg acaatcaaaa atacagcaag
     8221 aaaatttcct cttcctttta tacctaattt atcagatagc atttctaaaa ataaaaacga
     8281 agagaaagta taatacattg cgaagagaga atatggttta gaaaacctca gcgctgtatc
     8341 tgtatttcct ctgatgatga gtgaaatttg tgtcatcaaa ataatcaaac cgaataaaac
     8401 aatcaagctg ataaatactt gaggaagctt atagcggacc ttttctttcc agttccattc
     8461 tttcaaacca taacccatta aaaataaata aaaaatccat aaaatactct gtccattctg
     8521 taatgcaaac gggtctttat taaatagagt agagcagcca actaatccaa ttgttaaaaa
     8581 taatagtaga tatttaattg ttgttttcga taatttattt aaaaatccaa ctgtataagg
     8641 aatactacaa aaaagcaaag tacaagaaac agcataagta aagtaattct gactgatagg
     8701 tgttaggata agccaataat cacgaatatt caagctgcca gcttgtacgt aaaaaaagat
     8761 attgcaaact agtgtggcta ttaggaatat aaaggcaaaa cgacttaaat aatgtaaaac
     8821 ttttaaacgg gcgatttttt gatattggta acccatgtaa agtgggatca aatttgcacc
     8881 aataacaacg gaagtttgta aaaatgtata gatgtagaat cgaccagggt ttgttgcata
     8941 tgtaggaata gtgactgctg aaatcaatat acgctgtaaa accatcatta tacaagcgac
     9001 aaagattgtc aggaagccat aactctcctt ctttattttt ttcaagaata aacacctcac
     9061 aaaaaaattg gacaaaaaca taagaaaacg tttcctgata agttgcccat taataacatc
     9121 ctaacttaga atatctcaaa aaacaacgtg tagacagaaa aagtacaaaa ttagcaagaa
     9181 atggttgttt gtattgttag aatttttaga aaattatacc tttagtttct ttgcataaaa
     9241 tgatacaatg attgttaaaa ttgtgaaagg gtggtcattt gttagagaag tttttggata
     9301 tgtcgttagt tcaacgtatt gttgttggta ttttagtcgg aatattgttt ggtatctttt
     9361 tccccacatg gacatttatt tcaatattgg gaacgttgtt tgtcagttgc ttgaaggcaa
     9421 tcgcacctat tctggttttt ttcttgacga tggcgtcaat tgccaagcat aaaattggga
     9481 ataagacatt cgtgaagcct attttgatct tatatttagt gggaacattg ctttctgcac
     9541 ttgtagcagt tattgtaagt tcaattttta ctgttcctat tactttacaa gaaacagtat
     9601 ctgaaaaagc accgcaaagt ttggaaagtg ttttatctac tatgttgacg aatgtcacac
     9661 aaaatcctgt tcaatctctt attgatgcga attaccttgg cgtcttattc tgggctgtat
     9721 tgttgggact agcttttcga gcacgttcag aatcgacaaa agagttggtg gatcagatat
     9781 ctatagcgtt gtcacaagtc gtacaagtga ttattgcatt tgctccgata gggattttag
     9841 gattggttta tcaatcaatt gctacgactg gaatcgctgg attggcagag tatctgcagc
     9901 ttttacttgt tttgatcggt acgatgctgt ttgtcgcatt agttgtgtac ccattcctca
     9961 cgtttttgtt tattaaagaa aatccctatc cattgatttt cttttgtttg aaagaaagcg
    10021 cacttcccgc tttctttacc cgaagttctg ccgcaaatat cccgataaac atgatgttgg
    10081 ctgaaagatt gaaattgaca aaagagtctt attccatatc gatcccgtta ggtgctacga
    10141 tcaatatggg cggtgccgcg attaccattt ccttgatgac gttgactgct gtacattctt
    10201 tagggattga agcacctttt gttttgaaag tccttttatg tattttgtct gctttagctg
    10261 cttgtggagc ttcaggtgtt gcgggcggtt cactgttatt gattccatta gcatgtagtt
    10321 tgtttggtat ctctaatgat gtcgcgatgc aaatcgtcgg tattggattt attatcggtg
    10381 ttatccagga ttcaatggaa actgccttaa attcgtcaag tgatctgttg tttactgcaa
    10441 taggagagct aggcgcgcgt aaaagaagtg gggaaaaaat caaattgaaa gattgtttag
    10501 atggattaag tgaacgttga acgttagata tttagtattt ttaaaatgat tcgagttttt
    10561 aatgagttat atagatgttc gtgtaaatag aggtttctgt taaatcaaga taaagtcgtt
    10621 tataataaca attaaaagca taggtaattg ttattggagg cgtatcatgg attttttacg
    10681 taaatttggt tttttctttt tattattagg cgtgctgagt gattttttga ctccgtacgt
    10741 attaggattg tattatcctg aattagatca aatgaagagc gtgatcagtg tatttggaga
    10801 ggtttccagt cctgtaagaa aagcgtttct tatttggtct gtggtttcag ggatcttttt
    10861 catcctgtca ttgcctgcat tgtatgctgc atttgttagt acatctcgtg tgttagctac
    10921 cctagttaca gcagccatcg cgttctatgg tataggtgat tgtatcttta caggtttgtt
    10981 cagtatcaat acccatgaat cttcgtggaa tgtctcgaca tgggtccata atatcggatc
    11041 aggtttaggt tacagtggtt ttttattgtt tccagttctt cttttcttga tctatcgaaa
    11101 aaatgggcaa atggacgtaa gccacttata ccttggactg tctgtgatca gtttgctttt
    11161 tgcggcgatt tatggactgg caagaattcc ttctataaat tcttggccat tactgaataa
    11221 aataggtttt tgccaaagaa tcagtttttt ctttaattat gtgcccattg ccaggttgag
    11281 tatcttgcat ctcaaacagt aaaacttgag ccgtcaaatt gaacagtcag cggatacttc
    11341 atccggtggc tgttttttgt ttttctgaaa tatatatttc cacagttttc ttgtatagct
    11401 tctgtcttag agaagcaagg agctatattt tgtgctacaa tcttaaaaag gtagagagaa
    11461 gaccaaatgg aagacagagg agccactaaa atatgagaaa catcaagttg acaatcgaat
    11521 atgacggaaa aagatattca ggatggcaaa gacttggaga tgacgataaa accattcaag
    11581 gaaaaatcga aaaagtattg catcagatga caaatgaaga gatagaaatt attggatcag
    11641 gaagaacaga tgctggcacc catgcgcgag gacaagtagc aaattttaaa acaaacacag
    11701 aaattggact ggcagaaatg atcgacttta tgaatcgtta ccttccgcgg gatattgtca
    11761 tcaaaagaat cgaagaaatg cctgagcgct tccattcaag atataatgcg gtgggcaaaa
    11821 aatacagcta ttatgtttgg aacaacgtga ttccttcagc atttgagcga aaccacagct
    11881 tttatttccc tcaagagttg gatatggaca agctgaatgc agcttgcgaa aaattagtgg
    11941 gtacacatga tttcattggt ttttctgcat tgaaaaaatc aaaaaaatcc acagtgcgta
    12001 cgattgatga attatctatc gaacgaaagg gagaaatgct tcattttact tttgtcggag
    12061 acggattctt atataaaatg gtgcggatta tcatggggac attactagaa atcggcacag
    12121 gtgccatgcc gatagaaaca atcgatgaaa tatttgaaag caaagtgcgg caacatgcag
    12181 gagaaaccgt ccctgcacaa ggtctgtttt tggatgaagt ttattatcga taaaaatact
    12241 ttgataatgg atgaaacgca tgtttcacag tataaatatc gatgttaaat aatgtatgaa
    12301 gcaaattaaa gaaatataaa aaaagacttt ttagcctgtt tatgtgtaca gctaaaaagt
    12361 ctttcttaaa taaaaatcta gtgagaatgt tgtacttctc ttctcggttg tttttgttta
    12421 gaaaaagtaa atcgataaag taaaaataaa atcaaagtaa cgatagataa atatcttgaa
    12481 gcaattgcta gttttgtgta ctgataagta ataacgactt tatttatacc tggaggaata
    12541 gttaattcag ttgtgccaaa cttagataat tttgtttgta ctagtttgcc attaatagaa
    12601 gccacttgcc ctttataacg ataaataggt gtcgtcaaga cagtattttt attattcgaa
    12661 ttatctaatt caattgtaag tttgttactc ataaaatgag ttgttggatt tatttcctgg
    12721 ttacctagta aatattgatc attattaatc atatcaggat gatagattga ctctttgttt
    12781 gcataatcag tatgattgta attttcagct atagcaacag cgtcttcagg tgctataagt
    12841 gttaatgaat ctttgttagc atggatagaa ttgcttactc ctgagagatg caatattagt
    12901 gcaagaaacg taaagaccat tattttccat gaattatttt tagtagaaaa aatataatag
    12961 atcactatac tcatcgtata ggctataaat aaagaagaaa aaccatttaa ccgccagaca
    13021 aattgtagac tcttaactgg tgtatggtta aaaagttgcc aaggaaataa attggttgaa
    13081 caaaaaagaa caaatacacc aagaaagaaa ataaataaat catcagcagt taattttttt
    13141 ataaaaataa aagcaccaat caatccgaga aataagaata atccaatagt atagttgtta
    13201 tagttattat ttaaaatatg tgtcagcata tcactaggag ccataccact taattctttt
    13261 cccagtggga ctttcaattc ttgtgctcta gattgttgaa taaaaggaat taagaatccc
    13321 gcacttaaaa aaaaggtgag gacggtagct tttaaaagac ttaagagtct agcgattttt
    13381 tggtcccaaa aataaaaaga aagaaacaac gttcctccaa taaatacaga aaccatagcg
    13441 acagaaagta aatgagtata cactactagc gtcatgccaa tagacaacca gtaccatttt
    13501 tggtaatctc taatataaat ttcgtaacac cccattagta tcaaaggcaa aaatgttaaa
    13561 gcgacagctt ctcccaatga agcacgatgg aatatgtcaa tggcacgata ggcggaaaat
    13621 gtataaataa tcgaaaataa cagcgaaata taacgattgt tttttatttg tttcatagaa
    13681 aaataag
//
LOCUS       DAZKAS010000072        13399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000072 DAZKAS010000000
VERSION     DAZKAS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.074"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..713)
                     /locus_tag="ACG8H7_001730"
                     /pseudo
     CDS             complement(15..713)
                     /locus_tag="ACG8H7_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            792..968
                     /locus_tag="ACG8H7_001731"
                     /pseudo
     CDS             792..968
                     /locus_tag="ACG8H7_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="ACG8H7_001732"
     CDS             complement(1144..1713)
                     /locus_tag="ACG8H7_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP8983821.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="ACG8H7_001733"
     CDS             complement(1787..2110)
                     /locus_tag="ACG8H7_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983822.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="ACG8H7_001734"
     CDS             complement(2264..3076)
                     /locus_tag="ACG8H7_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983823.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3403..4278
                     /locus_tag="ACG8H7_001735"
     CDS             3403..4278
                     /locus_tag="ACG8H7_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP8983824.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4292..4615
                     /locus_tag="ACG8H7_001736"
     CDS             4292..4615
                     /locus_tag="ACG8H7_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983825.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4612..5364
                     /locus_tag="ACG8H7_001737"
     CDS             4612..5364
                     /locus_tag="ACG8H7_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFP8983826.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="ACG8H7_001738"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="ACG8H7_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACG8H7_001739"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="ACG8H7_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFP8983827.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="ACG8H7_001740"
     CDS             complement(9828..12014)
                     /locus_tag="ACG8H7_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8983828.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACG8H7_001741"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="ACG8H7_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFP8983829.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="ACG8H7_001742"
     CDS             complement(12236..12676)
                     /locus_tag="ACG8H7_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFP8983830.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="ACG8H7_001743"
     CDS             complement(12750..13289)
                     /locus_tag="ACG8H7_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP8983831.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DAZKAS010000073        13360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000073 DAZKAS010000000
VERSION     DAZKAS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.075"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..178
                     /locus_tag="ACG8H7_001744"
     CDS             11..178
                     /locus_tag="ACG8H7_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFP8983832.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(240..2051)
                     /locus_tag="ACG8H7_001745"
     CDS             complement(240..2051)
                     /locus_tag="ACG8H7_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFP8983833.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2048..2413)
                     /locus_tag="ACG8H7_001746"
     CDS             complement(2048..2413)
                     /locus_tag="ACG8H7_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP8983834.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2576..2971)
                     /locus_tag="ACG8H7_001747"
     CDS             complement(2576..2971)
                     /locus_tag="ACG8H7_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP8983835.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2989..3951)
                     /locus_tag="ACG8H7_001748"
     CDS             complement(2989..3951)
                     /locus_tag="ACG8H7_001748"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFP8983836.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3951..4163)
                     /locus_tag="ACG8H7_001749"
     CDS             complement(3951..4163)
                     /locus_tag="ACG8H7_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFP8983837.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4184..4882)
                     /locus_tag="ACG8H7_001750"
     CDS             complement(4184..4882)
                     /locus_tag="ACG8H7_001750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP8983838.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4902..5444)
                     /locus_tag="ACG8H7_001751"
     CDS             complement(4902..5444)
                     /locus_tag="ACG8H7_001751"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP8983839.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5576..6580)
                     /locus_tag="ACG8H7_001752"
     CDS             complement(5576..6580)
                     /locus_tag="ACG8H7_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP8983840.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6577..7566)
                     /locus_tag="ACG8H7_001753"
     CDS             complement(6577..7566)
                     /locus_tag="ACG8H7_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP8983841.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7563..8369)
                     /locus_tag="ACG8H7_001754"
     CDS             complement(7563..8369)
                     /locus_tag="ACG8H7_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983842.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8535..9491
                     /locus_tag="ACG8H7_001755"
     CDS             8535..9491
                     /locus_tag="ACG8H7_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8983843.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9568..10086)
                     /locus_tag="ACG8H7_001756"
     CDS             complement(9568..10086)
                     /locus_tag="ACG8H7_001756"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP8983844.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10174..10323)
                     /gene="rpmG"
                     /locus_tag="ACG8H7_001757"
     CDS             complement(10174..10323)
                     /gene="rpmG"
                     /locus_tag="ACG8H7_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP8983845.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10551..10997
                     /locus_tag="ACG8H7_001758"
     CDS             10551..10997
                     /locus_tag="ACG8H7_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFP8983846.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11192..13087)
                     /gene="asnB"
                     /locus_tag="ACG8H7_001759"
     CDS             complement(11192..13087)
                     /gene="asnB"
                     /locus_tag="ACG8H7_001759"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFP8983847.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4159 a         2832 c         2281 g         4088 t
ORIGIN      
        1 ggtggattca atgaaaaaat ttgtatctgg aatattagta ggaagtctgg caacagccgc
       61 tgccgttgct ggtttggttg cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa
      121 agaagcaatg atcgaagaaa accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata
      181 attatttaaa aatacgcatg aagactttct aaaaaacaga taagtcatca tgcgtatttt
      241 tatttgtcag tcaatataag ttgtgctatt ttatctgcct gtctcagatt ccatggatct
      301 gtataaggtc tagctactgc tgcagttagc caatccacac cttcaacagg tattccatag
      361 caataaaagt cagactggat ttcccctgaa gcatcaatca cgcgattcgt acctcgctca
      421 acatctatag cttctgaatg ataacttcct gtattatctg aaaatgaaaa agggcgaaca
      481 agtttgtttt ccttcaaatt ttttatgaca ggattacttg aacgagaaaa gcttgtttgt
      541 ggcaatcgag cttcaatcaa atagcgactg ctgatttctt tatcttgtag agaaataaca
      601 tagcggttgt gcttttgctg aatatcaaat ggcggttcga tcaaatgaaa aatacccgca
      661 tctatcagtg ctgctaattc tttcgtccga atgattggcg gaccgatcgt taaaaagcta
      721 tttagatgag taaaccatcc ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc
      781 cattcaatag cctgccggat tggatcacgc cagtctttta atgcatcaaa tgttgaagca
      841 aatgctcctg tgcaattgcc tttttccgct tcttttatct gcccttcaat aaatattcgg
      901 ctagcttctg aaaagggctc attctgatag gcaagaggag tttctaaata cgaccaatcc
      961 catctataag ggattagtgc tggatgcttt ccgagacact cctctgaaga gagggagaga
     1021 aaatcatgct caaaattttt ctttggaatc ggcaagtgat attcttcgat gattttcttg
     1081 taataaaaca actcaacgtc ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat
     1141 cccgactgat gccattgctc caggttttct tttgttagta accgtggcca tgccattgct
     1201 ccgccttgct tttgatttct tcctcgaggg taataaggga gcccccttct tgatccactg
     1261 tatacaatcg gttccttgcc tgatggatga tagaccagtt ttcccttttc gttctcgaag
     1321 cgccctcctc ttgatactgt aaacaatccg acataatcaa aaaaacttaa gcctagacct
     1381 cttaaaaaga cattttcttt aggcggtatc ttcgaaagat ctacatctgc aggattagaa
     1441 ggaagctgat acagtaaaac atttttcttt gcgtagtcta cccacactct ttcttcttct
     1501 gtctcatcat tttcccagtg accgcttgct aaaacaagct gatcgataga atagctttgc
     1561 tgatctgtct gtaaaagata accatctttt cttttgctta ctttttttac taaagaataa
     1621 acaaactgaa tagaagctgg aaattcttct tgcaactttg agaaaaacca gcgttgatag
     1681 agtccgtata aacatcgagt agattgctga tctttttcca gacgattgat ttcttctata
     1741 attagttgct tatttttggt taccgtctgt tcgatataat ctgtagcagg tcctttactc
     1801 cattgaaaca agttgggtcc ctgtgttacc aatcctccac tggacaatgt ttcatctgta
     1861 aaaagcgtca cttgttgaga aacagaattc attaataact ccggtggctg attcaaccgc
     1921 caaactcgac ctccaggacc attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc
     1981 tgattattat ggagaatcct ttctgctaca cttaatcctc taggtccggc accaatgatc
     2041 cctattttca tttgcatgcc tccatttcga taaatttttt caataaaaca gcatggttct
     2101 gatgttcatt ttttgcacca taaacaaaag taatgactgg atgttctttc ataagttgac
     2161 tgagtttaaa aatagcttgc ttcttttctt cttcgaaaat caattcttgc tggtatttct
     2221 ttgcaaatga agcaaatcgt tctggtacat ggtcaaaagt tttgcgtgct tcattactag
     2281 gtgcaatttc tttcaaccac agatctaact gcgctttttc ttttgaaatt cctcgtggcc
     2341 agaatctatc cacgagtatt ctatagccat cctctttttc agcatcaagg taaacacgtt
     2401 ttaatttgat catagttgtt tccttctttc taccttttcg cttagcttat atatgtagat
     2461 aatagcacaa aataaaaaaa atgaacggca caactatcca ttgcgccgtt catgtgatct
     2521 tggtcaaaat ctaatgattg attcggtttt taaagctgtc atacaatcat ctgttttata
     2581 cttttttact tttgattttt ccagaccatc cgtcatatcc acccttaagt aaataaatct
     2641 cttgataacc tgctttacgt aatttatttg ctgtacggat gctcaaactt ttcttttgat
     2701 cgtaaatata gacaggctgg tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt
     2761 aagggatgtt tcttgcacct aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat
     2821 cgattacttg tgcttttctc attccttctc gaaattcttc ttctgtcaaa gtcgttgctg
     2881 aacgtttggc cattactcgt agatagactt cccaaaggat gattgcaagc acaatcaata
     2941 gcaaaatgat attaattacc cataaaacca tgtaatgcct ccgttttttt aagcaaattt
     3001 caaggctaat gaggcagcac caatgacacc agcttcatta ccaagctctg ctaatttgat
     3061 ttttgttgat tctcttactt gcgggaaagt aaattcttta aaatatttct ctacacggct
     3121 acgtaaaaat tctcctgctg ccgatactcc tccacctaac acgatacttg acggatttaa
     3181 tgtattcccc aagtttccgc aagctaaacc taagtagaag catacgcgat caacaaccat
     3241 caatgcaaac ggatcgtctt cttgagccat ttcaaagata tctttacttg ttacttcttc
     3301 tccgttgtct agcattgctt tcagttttga atcccctgcg tattcttctg ccaagaaacg
     3361 acctaggcgt accacacctg tagcgctaga tacagtttct aaacatccgc gtttaccgca
     3421 agtacattcg aacccttcag gatccacagt gatatgtccg atttctcctc cagctccgct
     3481 cacgccgtga atcaaatttc cgccagcaac gatacctccg ccaacacctg ttcctagagt
     3541 aacgaataca acgtctggat cgttttctcc agcacctttc catcgttcac ctaaagcagc
     3601 aacattcgcg tcattatcga tagcgaattt gattcctgtt cctgcctcga tcagtttttt
     3661 cacaaattga acttctgtcc agtttaagtt atacgcacca ataaccgttc cagcttcgct
     3721 gtccacgctt cctggggtac ccatgccgat tccgataaag tcttcggctt tcatattata
     3781 taaattcaaa cgatgattga ttgattcaat gatttcagga acgatatgct taccatcatc
     3841 caaaatattg gtatcgatgc tccatttttg ctgaatctct ccttctggtg ttaaaattgc
     3901 aaattttgct gttgttccac ctaaatcaat cccaataatc ttcttgtcca ttacaaattt
     3961 cctttctttc ggctttcttc aattcggtgc tctcttttta atatgcgcca cgctgtcatg
     4021 tatgtatcat gatcaatcaa acggctatca tacagattac gcaattcgat agtcattaat
     4081 tcaatgtcat agatgcgtgc acccacatag ataaagatgc caaatttttt gaacagctgc
     4141 tgtacatcat ataagctctc catctttaag cacttccttc atattataca agtaaaccat
     4201 atttttttaa acccagtaaa atacatatta caaaaagaaa tacaaaaact agtgtagaaa
     4261 gaatcctttg gtgtatcgaa tagcttgtct gttgtttcgg tagacccaaa acattcccta
     4321 acactaatcc gccaattagc ccaccgacat gtccccaaac atctacagag gtatccagaa
     4381 gaccaaaaac aaatgtcaac acaataaata aaagaaaatg tctgactagc tgctggatcg
     4441 ctgggttatg tttaaaatga aaccctaaga taaacaacgc accgaacaaa ccgaatatcg
     4501 aagtacttgc accaccagac agaacattcg cttcgttaaa agcaaagctt gctgtatttc
     4561 ccattatccc gctgaacata taaatcaaga aaaaacgcca atggccatag attgcctcga
     4621 tttgctgccc cataaaatac agtatcagtg agttcattac gaaatgcatc agtccaaaat
     4681 ggatgaagat tggtgtcact aaacgccacc attcattgaa gtgaacaaga taaggaccat
     4741 acattccggc ataataaggg atctcaagtc gcggaaataa ttccatcatg aggaagaccg
     4801 ctgtctgtat ccctaaaaaa gcataggtcc ataacggtcg tcttaaccat gcacgcaatt
     4861 ttatttgctg ttgataattc atgaaaacct cccatttcat tttagtcaat aaacaattgt
     4921 tggatcttct gatcaaagct ctccggttgc cattgttcat tcaattgttc acgaaaaaca
     4981 aggctacatg tttttttatg aaaattattt aaataccggt cgtaataacc accgccaaaa
     5041 ccgattcgat aaccggctct attgtagata atacctggta caatgaggag atcgatcatc
     5101 tccggcaaat agagcttctc tgattgtggc tcaagaacac caaaactcga acgtacaaga
     5161 acggactgtt catccatttc ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca
     5221 actattttct tttgttcttt ttctgctcgt tggataaccg gctgtgtatc cagttcaaat
     5281 tcattcgata aagtcgtccc tatcactttt gctgacttcc agtactcgct ggcgaatagc
     5341 tgtttataga ttcgctgttc tttttccttt ttcttgccac ttttggccag atttttcagc
     5401 ttttcgattc ctttccggcg taatcttact ttcaattggt ccaaaaactc acccttcctt
     5461 ctctcataaa atggtatagg aaaaaaagaa aaatagcaat cgaactttca aataaatgca
     5521 taaatagaat aaaaagaaga cctagaaatg attggccttc cttttttaat gaatttcaac
     5581 tgttcagcgc gtgtttttgt ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa
     5641 gggcaatcaa aattcctgta ggtacttccc cacttggtag aatcactcta gcaaccatat
     5701 ctgcactaag cagaaaaatg gaaccgaaca aaccgctcag aacaaaggtc cagttatttt
     5761 ctttacgtac gactagcttc gcagcatgag gagctagtag tcctacaaaa gataaactac
     5821 ctgtcaccgc tacactaccg cttgccaaag caacagctaa caacaaaagg attttttgac
     5881 ttttagccac gttaaggcct aatccttgcg cgcgttcttg accaaatgat agcaatacaa
     5941 tcctctgtgc ataaagtcct cctagcggaa ttaagataac cgcccatgga agcaatgccc
     6001 aaacataagg ccagctcgca ccccagattg atcccgctaa ccatgcattc acaaatccat
     6061 aattttcctt agataattgg atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa
     6121 cggcaatacc ggacaataag attcgattca tacccaaaaa gccatttcga tcataggccg
     6181 caagatagac acagcaagca gctaaaacac cacctagaaa ggctacaact agcaatagaa
     6241 acggcagcga atagtgcgaa aagaaaccta aataaagcat cactaaaaat cctgctccgg
     6301 cattaatgcc caatatacta gaatctgcca attcatttct tgttactcct tgcaagatat
     6361 aaccagaaac gccaagacaa gctcctgcta ataaactgat cagtaatcga ggaactcgaa
     6421 attctctaac aatcagcgcc aaagaagatg attctttccc tatgaacgtg gcaaaaagct
     6481 gatccaggta aataaaaggc gtaccaatca ttaaactacc tatagacagg atgacaagta
     6541 caaaaagcaa gaaatagaac gtccaatata tttttttcac cgttgttccc tccttacttg
     6601 tatcagtaaa aacggaacgc caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg
     6661 gggattgatc atcctcgctg taaaatccgc caacataacc aatccaccgc caactaaaaa
     6721 agagacaggt agaacataac gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac
     6781 caacagccca ataaatgaaa caggtccgac taacgaaacg gcaatggctg ctaacactgc
     6841 aactagacag aaaacaatca accgcgttcg tttaggatga cttccaagag ctaatgcatg
     6901 tgtttcactc aaatgaatca aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc
     6961 aagtatatat aaaggaaaca atactgctaa ttgtttccat gtgatattgg cagctccgcc
     7021 tacaaaccaa aaagcaagat cctgctgcag attaaagaat tgggtaagtg cttggctgat
     7081 agcagtaaaa aaagaactaa tagctacgcc agctaaaatc agctgtaaag gattcatgcc
     7141 cattttcgaa aactgcaaaa aaccaaatac gagcaaagtt acagcaaaag aacctgccaa
     7201 tgacattaga aacacagaaa aatcagaagc tttaggccaa aaagcaaaac taagggccat
     7261 tgccaacgaa gcacccgcgt tgattcctag taagcctgaa tctgctaaag gatttcttgt
     7321 caccccttgc atcaatgcac cgctcaatgc aaaacaactc cctacaagaa atgctcccgc
     7381 cacacgaggc aaacgaatat ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc
     7441 aaacaacgcc tgccaaaaat ctgctttttc cacagtcgct gcgccatttg aaagagcaac
     7501 aaaaaaaata atcaaaagaa caaaacagac caaaagcagg agtctatttt tcttcatagt
     7561 cctcataatt gatcctcctg ctcttttggc aacaaatcat aggttagaaa aactggttta
     7621 tttgttcttg gaagcgtgac aatcgttgca tcaatctcaa atattttttt catacactct
     7681 tttgtgatca cttcttcagg tgtaccttct gtgatcaact taccgttttt cattgctaat
     7741 aaataatctg aaaaacgtga agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt
     7801 tgtttcattt gattgatttc agtcaataac tgtaggatct ctaattggtg tgccggatct
     7861 aaaaatgttg tcggttcatc caagatcaag atatctgttt cctgagcaag cgccatagcg
     7921 atccagacac gctgggtttg tccaccagac agcgttgcga tatcccgcat acgaaatcca
     7981 gctagacctg tagcatccaa cgcccactca attgcttcat gatcttgcgt gtccatgcca
     8041 ctgaacagtt tttgataagg gtatcgtccg taagatacta cttcttctac tgtcatacct
     8101 gtagggtgtt cactagtttg tgctaataat gcaatttttt ttgcaagttc ttttgaactg
     8161 tatgtagaga tatcagagtt attgacaatg atctttcctg atttcacagg tagtatacgg
     8221 gtaaaacttt tcaataaagt agattttcca cttccatttg gcccaatcaa actagtgatt
     8281 ttcccttcag gaatcgtaac agacatgttt tcaatgattt ttttgttttc gtagccggct
     8341 gcaatgtcag ctgctcttaa tctcgtcatt tcacccatcc tttcgctgaa aatcattctc
     8401 aattatgacg ctgtttcaac atttcgtcaa gcgctacttt catatctata gccaaatgaa
     8461 gcggttgaca gcgttcgaaa tatcaggaat aataaccaat gagaaagatt ctcaattatg
     8521 ttaggaggaa agaaatgaaa ttaaaaacaa aagtattcac attatctagc atattgctgc
     8581 tggctggagt actgagcgct tgctccccct cttcagataa agaaacagct acaactgcca
     8641 attcgacgac ttcatccaat agtactcata cagtcacaga tacacttgga cataaagtag
     8701 agattccaaa cgcgcccaaa cggatcatcg gcagttactt agaagactat ctgattgcac
     8761 taggggaaaa acctatcgcc cagtggactg ttggcagtgg ttccattcaa gattatctgc
     8821 aagatgattt aaaggatgtc ccaacgattt cttatgacct tccttatgag aaagtgttaa
     8881 gctttgagcc tgatttgctg ttgatttctt cttccgctac tgtcgaaggc gggaaatacg
     8941 aacaatatag caaaatcgcg ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg
     9001 aagaacaact aaaagatgtc ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca
     9061 tttccgatta tcaaaaatcc atcaagaaaa ctcgagaaga actagcagat aaaatcgaga
     9121 acaagacagc tgcagtgttg tgggtgacca acaactcagc ctttatggtc agcaaaaatc
     9181 ggtcaagtgg aagaatcgta tacgacgatc tggaatttgg tgttcctgat ctagtggaag
     9241 aggtctccaa aaaagccact tctgactggt ctgctgtttc ctctgaaaag ctagctgaat
     9301 tagatgctga ctacatcatc cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac
     9361 caagctggaa gaatctaaaa gcagtgacag acaatcatgt actgcaattc ggacctgaat
     9421 ctagttggct atataacgga cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa
     9481 aactaaattg attttaacta aaaaaagcat cttcacgatt agtcgtgaga tgcttttttt
     9541 cttattaaca aactagacgc atagatttta ttcttctttt gaagcaaccc tattttttac
     9601 tctttcccat ttttgaaaac gatgggcata tagattttct tgatccgtta tcccctcata
     9661 ctcctctata agggcgaagt ctgaaaaatc aatattccca atatacgtat cgccttcaaa
     9721 cgtgctatcg atcaatgttc gccagatccg accagtgtaa ggaagaagtt cttgaaaaat
     9781 ccgactacca cccgaaacat agatatcttc tgagatcgtt tgactatacg acaaaagttc
     9841 atcgatcgaa tgcattactt ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa
     9901 cacgatgata tggcgataag gaagtgaaag atcccccatt ccttcatagg tctttcttcc
     9961 catcactaaa attcgatcca ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg
    10021 ccatggtaac aaaccatttt tgccgatcag accattttta tcttgtgccc acatggaaat
    10081 aaacatatta tccgctcctt atgattttag atgttttgag ataaaaaaag tctcaattct
    10141 ttgaccagca aggaattgag aactttaaaa acattatttt gtttcacggt gcaaagtaac
    10201 tttgcgttcg cgtgggcaat atttttgttt ttccaaacga tcaggattgt tacgtttgtt
    10261 tttgcttgtt agatagttac gttctttaca agaagtacac tctaaagtga tgtttacgcg
    10321 catgtttttc cctcccattt ttacaagaat cctctaagat gactctcaga cttgattatc
    10381 ttaccatgat tttttctaga ttgctagtgt ttttcacgat tttgttaagt aaaactactt
    10441 gacgataaat acaacggttt tacttctcaa acaaaatctt tttctttttg tataaatacc
    10501 cttacacttg tttatataga ttatgttttt taataaaggg ggatactaat atgccatgga
    10561 acatgaagga ctttcctgca tcaatgaaaa acttagataa attgacaaga aaaaaagcaa
    10621 tcgacatcgc aaatgcatta ttagatgaag ggtaccctga taaccgtgcg atcccaattg
    10681 ccattgatca agcaaaaaaa tggtatgaag atgctagtga atcagaaaaa cgaactttcg
    10741 aaaaagaaaa aaatccaagc aagacggatg aacacgatac gaatcctcgt gctggaaaac
    10801 tgcttgattc agatgtgatc gtagaatatg aggaagaaca atggatcgtg aaatctaaag
    10861 gggcaaaaaa ggcaagcaac cattttgata caaaaaaaga agcaatcgaa aaaggaaagc
    10921 aagtagcaca aaacaaagaa tcaactttgt tcatttataa aaaagatggg acaaaagaaa
    10981 aagaaatcga ttattgaaca tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc
    11041 ttatgttcga aacaatggtg ttcctctgcc aactccgaac cataagtcat aatatctata
    11101 aaacgggaag agctgttttt tgaaattgtt tcttatcact cagtgttaaa cggtaggaga
    11161 aaacctcttt tttattgata tttgattttc atcaaacagt cacatagtcg attgcttctg
    11221 cttgcggaat cgtttcatct acaaagtaga ctttatacca tgtcaagaaa ctgaagattg
    11281 tccagatttt tctttgctct tctttacgtc cttcgtgatg gtcatctagc aacttcatga
    11341 ttttttcttg atcaaaaaat tcttttgcaa aatctgcttc gaataatgct ttgacttgtt
    11401 cgtagccgtg ttcttctttc aaccaagctt tgattggcac agggaatcct aatttcacac
    11461 gattcgacca ttcttccgga agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt
    11521 tgttttgatt caataaaaac ttacttggta tttgttgagc caatttcatg acttcgatat
    11581 ccaataaagg tactcggact tccaaagaac ttgccataga aagtttatct gcctttaaca
    11641 agatatcttt tggcatccat tgatgaagat cgacatattg catcttattg acttcatctt
    11701 tgattccttc tgttttctcg taatgaacag tcatgatttc atccacagat ggtgctgttt
    11761 gatattttgg tgttaacact tctgatgctt ctgattcttc gaacactcga gcatgaccga
    11821 taaaatattc ttttgcagga gcaagtgatt catataaatg gatccgtcca tggaagtttt
    11881 tcatttttcc gattttacgt cctaaattat agcgtgttcc ttttggtaat tttttcaatc
    11941 cttgagcaat cacacggatg aattttgaat tcgtatggaa tccatacgtt tggtatcccg
    12001 caaacagttc atccgcccct tctccagatt ggactacccg cacactttgt gaagcaagat
    12061 ttgctaaaaa gtacaaaggt acgcaagaag gatttgaatc aggttcatct aagtgatact
    12121 ggatcaaagg gaatgctttg aacgacatgt ctccgtcgat cactgctgct gtattatcta
    12181 agtccaatag ttcggttagt tttctggctt caatcgcttc attataggtt ttatcatcaa
    12241 acccgattga gaaagaatga tctggtttca atacagaggt tacataactg gaatcaaccc
    12301 cgctggataa gaaagaacca acttctacat cactgaccgt atgtgcttca atagaagctt
    12361 gaacggtttc gtcgattttt tcgatccatt cttgtcggga atgcgtttct tttgtttcaa
    12421 aatctgcatc ccaatattgc tgaatgtcta tttttccatc tttatacgta taataatggc
    12481 cttcaggcaa acgatagaca tctttgaaga atgtttcgcc gtttaatgga gaatattgga
    12541 aagtcatata aggtttcaac gcttctttgt tcaattcttt gttaaaatca ggatgaggca
    12601 gaaaactttt gatttctgaa ccaaacataa atgttccgtt catttctgca taataatatg
    12661 gtttgatccc aaagtggtct cttgcaccga ataactcatt cttttcatta tcccaaatag
    12721 caaaagcaaa cattccgcgg atcttttgca ataattctgt tccccattct tcgtaaccat
    12781 gcaaaaggac ttctgtatct gcatgtgttt gaaaaacgtg gccagctgca atcaattctt
    12841 ctctcaaagg ctggtaatta tagatttcgc cattaaaaat aatgatcttt gtccgatctt
    12901 cattgtaaat aggctgagta ccgccctcta agtcaataat acttaatctt ctgaatccta
    12961 aagcaacatg tttatcgata tattctcctg aactattagg tccacggtga acgattcgat
    13021 ccatcattgt attaataatt gtttttttgt tgtccttgtc gttaacaaaa cctacaatac
    13081 cgcacattta actttcacat cctaatataa aattactgtt tatttcattc atatgcatct
    13141 tatcatagaa taaatagcgg acgaatattt tctctcttac aatttcgtca cgtgaaaaca
    13201 aaagatgaaa gtttctgttt ttacagaaaa taaagtccca atatctaatt taccatgttt
    13261 tcttttttga ggctacctta ttctcttcgt gttaaaaaaa tattagtgga tgctatttcc
    13321 aaaaaaacca gcaaaaaaag acacgaccta aagatcgtgt
//
LOCUS       DAZKAS010000074        13039 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000074 DAZKAS010000000
VERSION     DAZKAS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.076"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..1093
                     /locus_tag="ACG8H7_001760"
     CDS             191..1093
                     /locus_tag="ACG8H7_001760"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP8983848.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            1306..3081
                     /locus_tag="ACG8H7_001761"
     CDS             1306..3081
                     /locus_tag="ACG8H7_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFP8983849.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3147..4733)
                     /locus_tag="ACG8H7_001762"
     CDS             complement(3147..4733)
                     /locus_tag="ACG8H7_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP8983850.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            4984..5208
                     /locus_tag="ACG8H7_001763"
     CDS             4984..5208
                     /locus_tag="ACG8H7_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFP8983851.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5350..7101
                     /locus_tag="ACG8H7_001764"
     CDS             5350..7101
                     /locus_tag="ACG8H7_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP8983852.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7101..8885
                     /locus_tag="ACG8H7_001765"
     CDS             7101..8885
                     /locus_tag="ACG8H7_001765"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8983853.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9211..10062
                     /gene="licT"
                     /locus_tag="ACG8H7_001766"
     CDS             9211..10062
                     /gene="licT"
                     /locus_tag="ACG8H7_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFP8983854.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10155..12041
                     /locus_tag="ACG8H7_001767"
                     /pseudo
     CDS             10155..12041
                     /locus_tag="ACG8H7_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(12081..12983)
                     /locus_tag="ACG8H7_001768"
     CDS             complement(12081..12983)
                     /locus_tag="ACG8H7_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8983855.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT         4163 a         2317 c         2706 g         3853 t
ORIGIN      
        1 tcttttttaa aagagttaaa acgtgagaaa atgatcttta ttataaaaga gttcttaagc
       61 ttttctttag agaaaatttc agttactatg gagaacaggg caaagaaaaa tcaatcattt
      121 tctttgtata tcatcttacc aatatatagg aattgttagt aacaattgcc tttcaaggag
      181 gaatccatcc atgttattag gctcacacgt cagcatgagc ggaaagaaaa tgctgttagg
      241 agcagcagaa gaagcagcca gttatggatc ttcgactttt atgatctata caggcgcccc
      301 tcaaaacact agaagaaaag ccatcgaaga gatgaatatt gatgccggaa aagcgtacat
      361 ggcagaaaaa ggtctaacaa atatcgtcgt tcatgcccct tatatcatta atttggggaa
      421 tacgatcaaa ccagaaatgt ttccttttgc cgtccaattt ctgagagaag agattgttcg
      481 tgcggaagct ttaggcgcta ctcaaattac acttcaccca ggtgctcatg taggggcagg
      541 aacagaagcc ggaattgcta gaattattga aggcttgaat gaagttttga caaaagaaca
      601 gacggcacaa atcgctttgg agacaatggc aggtaaaggt acggaaatcg gtcgaacatt
      661 tgaggaatta gcgcaaatca tcgatggagt aaaattgaat gaaaaattat ccgttacttt
      721 tgacacctgt catacgaatg atgccggtta taatattaaa gaagactttt caggtgtaat
      781 ggaagagttt gataagatca tcgggttaga tcggcttaaa gtactccata tcaatgattc
      841 taaaaatccg caaggttcgc ataaagaccg gcacgccaat attggttttg gaacaatcgg
      901 attcgacacg ctgaataaga tcgtccatga tccaatgttt gacaatattt cgaaaatctt
      961 ggaaactccc tatgtaggca aagataaaaa gaaagcttac gcaccatatg gaaaagaaat
     1021 cgccatgttc aaagcgcaaa cattcgatcc agaagtgttt cctgagttat tagcagaaga
     1081 agtgatactc taagattttc tatctgacag cagcaaaaaa ggaccaggca cattgccttg
     1141 tccttttttg tttaagaaac aaaggagtac tttttgcagt ctttttcatc atgcatcttc
     1201 tcccccctct ttttaaattt agacaaacaa ggtatactgg tagaaaagta aaaaaacgaa
     1261 aagtttcaca agcaagtggc aaaacgtgat tggaaggacg aaaagatgct gaacacttta
     1321 aaacccttga tcaaccaact aaaaaagaaa catacatact ttattcttgg catagcccta
     1381 ttatttacaa ttattcgttt gtatttatat agtaaagcaa tctatggaac agacatgtcg
     1441 gcaacctatg atgaccagct gctgattcgc tatgctcaaa atattttgga cggtaaatgg
     1501 ctaggggagt atacgactac aacactttcc aaaggaatct cctacagctt atttttagta
     1561 cttgcacata aactgcatct ttcttatgca gttttgttcg gcatgttcag tatttttgcc
     1621 agtatattgg ttgcactcgc gtttaaaccg attgtgaaga acaagtatct tcttttaagt
     1681 atctattttt attttttgtt ttctccgatt agttttacac atgaatattc cactcgaaca
     1741 tatcgtaatg ccctcgtgat tccggctgtt gagatcatta tcgcttgttt attagctatt
     1801 tattaccgca aatacggttc tagcagaaaa ttgctgccat ggtttttagg gctttctgtc
     1861 gcttttcctt tcttttggtt cattcgcgaa gattctatct ggcttcttcc ttttactgta
     1921 gtagctttgg gtatcagttt cgtccagatc ttgctagggc aagacttttc tttcaaagaa
     1981 gtaaagaagt taaaaaaacg actctttctt ctctcgaaac gggaatggct gaaactttca
     2041 ttgtttttac ttcctatagt tggcttttcc atcgtctatc aatggatcga atataaaaat
     2101 gagacaacat acggtattgc aaccatcaat gaccgttcca gtggagaatt tggacgtttg
     2161 atgaaacaac tgatccgaat agatgatgaa acgaacttga ataaagaaaa cagtgagata
     2221 tgggtctcac atgctgcact aaataaagca ttagccgcat ctcctacatt ccatactgtt
     2281 tctacccgta ttgacgaatt atatacgact cacgcgtgga ctcaaggtgg aaaagtaaaa
     2341 gaattacccg gagatatcat cttttgggca ttgcgagatg tggtaagcga atctggttat
     2401 taccaaaaca atgcaaaaga aaccaatgat ttttggaaaa aagtcaacca agaattagaa
     2461 gaagcctatc acaagggaac aatcaaaaag aaaaaagagt tctatctgat gggatcagga
     2521 gatggaaaga cgatgagtga tctgccaatc gtattcgatt tccttcaatc aggatacgct
     2581 tatgcatggc gatataaaga tttccatcaa ggaggaaatt atagtttcgg aacgcaagaa
     2641 caagtcaaac aggctcaaga attattaaac cttccgctaa tggagaattg gatcgatagt
     2701 aatgaaccga gaacacctaa tttccaatta acaaaatcag caaaagtcgc gaatgtcatc
     2761 ataaaaattt accaagttct ggctattccc gtgctgattg cttcaatagc agggatatta
     2821 cttatgatac taggaaccgt atttgcaaaa gctgatcgtg cctattatag ttcctctttg
     2881 attgtggtca ccggattaat actgacacaa ttggtttttc ttttcggtgt ctcatggttt
     2941 tgttcttatg cgccagaaaa aaaggactat tttttaagtg tctacactgg tgcaggcgtg
     3001 ccattaacgc aattcatcgt agtaacaagc atgctagttt tcagtaaatt aaatctgaaa
     3061 aaagctttat cttctaaata aaaagaccct tggtagctag atttttaacc taactatcaa
     3121 gggtctttgt gtggaaaaat cagtttttat tcgatttctt ctaccgtttc ctgcaaaagc
     3181 tgttcatatt ttttgtttcc tttatataaa tcaccaatcg tccatgccaa caggagaagg
     3241 acaagaacaa tgattacata agcgatgtga tcagccaatg ctagttgagc ggaagtggct
     3301 tgctcgccga agaaactttc gatctgtcct ggtaatcctc gtaagttcaa gtaagttaga
     3361 ctaatcacag agaaccagcc aagaagtttt acgatgaatg aatttttaaa tcggctaccc
     3421 atctccgctt ggctgttggt catcatcagt aaaggaatca tcgaaaacgg caacgcaaaa
     3481 gccaagaaca cctgggattc attcattaag ttattcaaag cggcatgttc ttcgatcgtt
     3541 ccttttgtac ttgtaaacaa aacacaaatc aatacaggaa tcacagaaag caatcgtgtg
     3601 accagtcttc ttagccaaat cgggatacgc atatggatga atccttccat gatgacttga
     3661 ccggtcaacg taccagtaat agtagagttc tggccagaag caagaagtgc tacagcaaat
     3721 aaagtggaca agataccaga ttttgctaca gcaatcaaaa taccattgct catagctgat
     3781 ggatcagata aagcttcata cagaccaaaa aaggaaggat cttggacaga accggttttg
     3841 aagacagcga cacccatgat caatagcagt gcattgacta gaaaagccat tgacaactgg
     3901 atattagaat cccaagtaga aaaacgtaca gcttgtgcta catcttcttc gttttggtga
     3961 tcgatcttcc gagtttgtga aactgctgaa tgaagatata agttatgcgg catgactgtt
     4021 gcaccaataa tccctaaagc tccagtcagc ggagtttcgc cgccgatgac cggtgagtct
     4081 gagaatgtat gactgttagg gataaagcca gcaaataaac tgtgccaatc tgggttagac
     4141 aagccaactt gatagacaaa aacgaataaa atgacgaaaa ttaagcagac aaccagtgct
     4201 tcgatcttac gaaacccgac ttttgtcagt agtaagagaa cgaaaacatc taatacagta
     4261 ataaagacgg caatcaccaa tggaatatca aaaagtaagt agagggcgat tgctgcacca
     4321 atgacttctg caatatctgt tgccataatg gctaattcag tcaagatcca taaaatgaca
     4381 cctaatgtct tacttgtgcg tgctcggatt gcctgtgcca aatccatttg gctcacgatt
     4441 cctaattttg ctgccatata ctgtaaaagc atagcaatca gactagacat caaaataact
     4501 gacatcaata aatattggaa attttgtcct ccggtgatcg aagtagacca gttacccggg
     4561 tccatatacc caactgctac taacgcacca ggtccagagt aagcaagaag tgttttaaaa
     4621 aaacttttcc cctttggtac atcaactgtt ccatttactt cagctaaaga taaaccattt
     4681 gtatgttcaa taagtcgatg cttatgaaat tgtttattgg attgattatt caagaaaaaa
     4741 ccacctttcc tatttatcta cgattacatt ctacacctca aaaagaaaaa attaaagaaa
     4801 aagattcggt acgcctaatt ttttttcatg gcgcgtcttg ctagatgacg aaagaaattt
     4861 tgttctcctc taaaaaaacc actatttttt gcatgaaact aaagaatacg cctttctttt
     4921 ttcaataaac tttagtaaaa taagtcattg agagcaattc ggaaatagaa aggagagatt
     4981 tatatgttat caacaggtat tgtcgtactt attgctatta ttgctttgct aatcggcgca
     5041 gtaggtggct ttttcctagc gcgtaaatat atgaaagact atttagaaaa aaatcctccc
     5101 gttaatgagg acatgttgcg atcaatgatg atgtcaatgg ggcaaaaacc atctgagaag
     5161 aaaattcgcc agatgatgca gcaaatgaag aaccaaggga aaaaataggt tctgacccaa
     5221 cgacttttta cgggctgatt ttttcagtcc gttttttatt tcgtaatttg ttagactatt
     5281 cagattattc agcaaaaaag acaagcacag acaatcctag gctcttgata aagacaaagg
     5341 aggagtaaaa tgtcgatttt tagaaaatta ggctggtttt ttcgtcagga aaagaaacat
     5401 tatttaattg gggttttctc gctattcgca gtggccttgg tacagttagt tcctccaaaa
     5461 gtgattggga tcatcattga tgagatcgcc gataaaaatt tatcaataaa gatcattttc
     5521 gggtggatct tcctattgat tatcgctgct tttttacaat atctttttcg ttatatctgg
     5581 cggacgaata tttggggaag tgcagctcga ttggagaaag aacttcggca gcagttattc
     5641 aaacatttta cagaaatgga caatctcttt taccaaaaat atcggactgg ggatttgatg
     5701 gctcacgcaa cgaatgattt gaatgcgatc cagaatgtag caggagctgg tatcttgacc
     5761 tttgcagatt cagtgattac gggcggaaca acgatcattg cgatgatttt gtttattgat
     5821 tggcgcttaa cactgattgc gctgattcct ctgcctttgc tggccgtcac ttcacgagtg
     5881 ctggggtcaa aactccatga tgcttttcgt gattcgcaag aagctttttc tagtatcaat
     5941 gataagacac aagaaagtat cactgggatc aaagtcatta aaacatttgg acaagaaaaa
     6001 gaagatctag cggattttga agaaaagatt gaagatgcaa ttattaaaaa caaacgagtc
     6061 aattttttag actccttatt tgatccattc attaccttga ttatcggtct gtcttatgtt
     6121 gcaacaatta ttttaggtgg aaagtatgtc ttggaaggca cgatctcgat cgggcagatg
     6181 gtctcattta ttagttatat cggtatgctt gtttggccga tgtttgcgat tggtcgattg
     6241 ttcaacgtat tggaaagagg aaatgcttct tatgatcgcg tcaatgaact tttgcatgaa
     6301 aagacgcata tcgtggaaca actgggtgca gtgcagaccc cggcaaaagg cgagctgaat
     6361 gtagctattt cctcttttaa ttatccgaaa gaagaatcac cagcattaga acaaatcaag
     6421 tttgcgatcc aagaaggaga aacattaggt attgttggga aaactggtgc gggaaaaacg
     6481 acgatcatca aattactgat gcgtgaatac gatgattatc aagggaaaat ccaatttggc
     6541 ggacgttcga tcaaagaata ttcattgaat gctttattag gatcaatcgg ctatgtgcct
     6601 caggatcact tcttgttttc gatgaccatt cgagataata tccgtttttc taatccaaaa
     6661 ctttcagaag aagaagtaga gcatgcggct tctttagcat ttatcaacaa tgaaatcaag
     6721 gaattaccaa aaggttatga taccttagtt ggagaacgtg gagtctcttt atctggcgga
     6781 cagaaacaaa ggatttcgat tgctcgagca ttgatcaaaa atcctgaatt attaatatta
     6841 gatgatgcac tctctgcagt agatgccaaa acagaagaag ctatcttgca taatttaaaa
     6901 gaaataagaa aagagaaaac aactatcatt gcagctcacc gtttaagtag cgtgatgcat
     6961 gcaaaagaaa tcattgttgt cgatcaaggg aaaatcattg aacgaggaac ccatcatgag
     7021 ctgcttgcac taggcggctg gtatgaacaa atgtgggaga aacagcaatt agaagcgaag
     7081 atagaaggag gcgaggcctg atggatgaaa attatcaatc ggaatggaca aaatcgattc
     7141 cacttaaaga acagaaaaag atcatcaaac gactgatgaa gttcgccaaa ccttttagaa
     7201 gaacgttcat cgttgctatc ttatttgcgt ttgctttgtc tgtgatcaat gtcctattgc
     7261 caaggatcat ccaaactttt atggatgatc atctggctaa acagtcggct acgacacaag
     7321 ttattttgtt ttttgctggt atgtacttat ttggagtcat agtaaaaagt atcatttggt
     7381 ttttccaatg gtatttgtat tccatggcct cattgaagac ctatcagtat attagggtaa
     7441 aattatttga aaagctgcat acactaggta tgcgttattt tgatcagaca ccggcaggat
     7501 caaccgtttc tcgagtgacg aatgacacag aaacgctatt tgaattttgg tacgtctttt
     7561 tgatggtatt gactgggatc tttgcagtcg tttcttcatt ccttgccatg tttcaaatca
     7621 atggagaaat tgccttgtgg actttggtgt ttttgccgat tttaggaatc gtcatctggt
     7681 attaccagaa attcagttct cgtatttacc gaaatatgcg ggaaaaatta agtcagctga
     7741 acacgaaatt gaatgaatcg atttccggca tgcgtattat ccagcagttt cgccaagaag
     7801 ctcgtttagc taaagaattt gaacaagtga acaatgagta tttagacacg cgttatgcga
     7861 tgatcaaaac gaattcgatt ctgttaagtc cgatcatcaa ttttctatat gcattggcga
     7921 ttgcgttggt gttgactttg tttgggatcg atgcgttgca ttctcccgta gaagttggac
     7981 tgatctatgc atttacgacc tatgtccagg gattttttaa tccaatgtct caaatgatgg
     8041 actttttgag catcttcaca gatgggatgg ttgctggtag tcgtatcttg aagattatgg
     8101 atacggaaga atatgcacca caacaaaacc ctggagcaga tcaaatgatt acttcaggta
     8161 agattgagtt tcgcaatgta agcttttctt atgatggaga gcacaatgta ttaaacaata
     8221 tctcttttgt tgctaatccg ggagagaccg ttgcattagt gggacacacc ggaagcggaa
     8281 aaagctcgat catcaacgta ttgatgcggt tttacgaatt ttatgaaggt cagatattga
     8341 ttgatgatca tgacattcga gaatatccaa tggaagagtt acgtaagaaa atggggctgg
     8401 tcttgcaaga tgctttcttg ttttatgggg atatcgcagg aaatattcgg atgatgaatc
     8461 cagctatcac agacgaacag atacgccatg cggctgagtt tgttcaagca aacaaattca
     8521 tcgatgaatt accaaaagga tatcactcaa aagtgatcga acatggtgcc agctattcta
     8581 gtggacaacg acagctgatc acttttgcgc gaacaatcgt gactgatcct aaaattctag
     8641 tattggatga agcaacagca aatatcgata ctgaaacaga aggattgatc caagaaggat
     8701 tggcaaaaat gcgtcaagga cggacaacga ttgcgattgc ccatcggctg tcaacgattc
     8761 gagatgcaga attgatttta gttttagaca aaggtcatat agtagaacgc gggaatcatg
     8821 aaacacttct agcgcaaaat ggtttgtatg cagatatgta caaactgcaa agcagtggta
     8881 tataaaagat gttgtgacag tagaattttg acctaagcac tagagtgaaa ataaaacata
     8941 gcttctcata tttttcatct gtgagagtta gtgtcgtagt caccagattt aaaaaaagat
     9001 tcgacagcta gtcagaagtg cctaatagcc actaagaaag cgttgacaaa agcatttgct
     9061 ttcgataata taaagaaaac gatacaactt ggatagtgat tgcctgatgc aagctaaacc
     9121 tgatgagtct cccatggttt catggacggc tttttgggtt tttctttttg ttttggatac
     9181 agccctatac atcatggaaa ggagcaaaac atgctgattg agaaagtact gaataacaac
     9241 gtcgtaataa ccagggatga aagaaatcag gaaatgatcg tgatgggaaa aggtttggca
     9301 ttcaaaaaga aagtgggtga cccgattttt gactcatgca tcgacaaaaa atttcagttg
     9361 acaaatgacg ggctttcaca acagttccaa gaattggcac aagcgattcc tcttgagtac
     9421 ttagaaattt cttgtgaaat cattgaatat acaaaagagg ctctacaagt ccagttgaat
     9481 gattctgtgt atattgcatt gacggatcat ttgtatacgg ctatcgaacg tcacaaaatg
     9541 ggaatatttg taccaaatgt attgctgtat gatatcaaac aactatttcc taaagagtat
     9601 gccatcggga aaaaaacagt gaagaaaatc tcggataaat accacatcaa gttatcagaa
     9661 gatgaagccg gatttatcgc attgcattta gtcaatgcac aaggcattca aagccagcaa
     9721 gacaatacct acctgtttac tataacaata caggatattc taaatatcgt ccgctattac
     9781 tataagatta catttgatga agattcagtc catttttttc gattcgtcac acacttacgt
     9841 ttttttctga cacgcgtcct agatggagaa aaatcagaag tgggagaagt ttctgaggaa
     9901 ttgtttcagg ttattcaaaa aaaatataag gatacgtttg gttgcgttga gaaaatagca
     9961 gactttttag ccgagacgtt caactaccag atgtccacgg acgaaaaaat gtatttaacg
    10021 atccatactg cgcgtttagt gcatgagaat cgttgtaaat aaaaaaagga ggataaatta
    10081 acaagaacgt atgaaataag ggtagtgaca ttcagttggc taaaccttcc actaataaat
    10141 tggagggaat agaaatggga aaataccaag aattagcgag aaaaatcgta gaaaatgtcg
    10201 gcgggaagga aaatatcaat ggtttgacac attgtatcac aaggcttcgt ttcaaattaa
    10261 aaaacgaaga aaaagcaaat gatgagatct tgaaaaatat ggacggtatt gtaacggtaa
    10321 tgcgagcggg agggcagtat caagttgtca ttgggaatca tgtgccagtc gtgtttgaag
    10381 aggttataaa agcaggaaac cttacttttg atgaggctgt ttcaacagaa aaaatgagac
    10441 cgtttgatat gcttattgat ggaatatcgg gctgtttcca gccattcctt gctatcctag
    10501 ctgctggcgg gatgattcgt ggactgacag cattccttgt cttcttaggc gcgtttgacc
    10561 gcggttctgg cacatttgtt atgtttgata atatcggtga ttctgtattc caatttatgc
    10621 ctgttattat cggtttaaca gctgcccgta aatttaaagt aaatgaattc gtagggatgt
    10681 tgcttggcgc agcattaatg aatcctagct tgtcgctgga agctttgagt ggtgcagcag
    10741 aagcaccact aacgaccata ttttctggca ctatttttga agcacctatt tatcaaacag
    10801 tattcggcat tccctggatt gcgcgtaact acgcttcttc tgtcattccc attatcttta
    10861 ttgtgttgtt agctagccaa attcagaaac caattaaaaa gttagtacct gagatgatcg
    10921 cgaatttctt cgttccattc ttcaccgttc ttattacaat gcctttaggt tttttactcg
    10981 ttggaccagt attcacattc gcaactgata ttttaatggc tggatttgaa acacttttag
    11041 cactttcgcc agttatttat ggagcaatcg taggcttctt ctggcaaatt ctggtgatgt
    11101 ttggtctcca ttgggcaatc gtaccaatgg gattgatgca attttctgtg aatggttggc
    11161 aaaatattat gacacctgtt gcagtagtgt cttttggaca aactgcagca cttaccgcac
    11221 tctactttaa gcttagaaat ccaaaagaca aagcaattgc aataccagcc attgtttctg
    11281 gtattgtggg gattacagag ccagctattt atgggtttac attaccacgt aaaaaaatat
    11341 ttatttttac ctgtgtcggt ggtgcgttag ggggcgcgta tagtgggtta atgaatttaa
    11401 cttcttggaa tcaaggtgga ctaggcatct ttacaattcc caactatatt agaccagatg
    11461 gtgatttaac agatgtgatt aatgtattga ttgggatcgc cattgcgatg gtggtatcct
    11521 ttacgctgac ttttttcttt tggaaggatg aagcaggtga aacagatgat atacagaaaa
    11581 aatcaggtaa agaaattgta aaaacaccga tacaaggaca aatcgctcca ctaaatgcag
    11641 caaaggatgc agcttttgca caaggaacac taggtcgagg aatcttgatc taccctgaaa
    11701 aaggagaagt acgtgcccct tttgacggaa cagtcatgac cctgttcccg acgaaacagg
    11761 caattggaat ggtttctgaa acagggctgg aattgctgat tcatgtcgga ttggatactg
    11821 tgcagttgga agggaaatat tttgaaagct tagtgcagca aggataaaga gtgaaacaag
    11881 gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa ggaaggatac agtttagaaa
    11941 cacctgtgat cgtcaccaat tacacggatt atttggatat tttggaaaac caaggggaga
    12001 cggtcgggcc agacgatgca cttatcaccg cactgacctg aacggttagg tgaagaatgg
    12061 actaggctta actattcttt ttattccact tctttatttt caaaattgaa aggctctttc
    12121 atgatggctg agctgatagg gcgtttatcg aatttttgac cgacagaaat tagatggact
    12181 aaattctgtt gataattttc tgcaaaaaca caactatata acacgtctag aagataacaa
    12241 attgatgtat tgatcgtaaa attaccaatt tttgaataca gtcgctcgcg tgtcgtaagt
    12301 cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac cgatactggt caaagctaaa
    12361 attggaacat gattttgttt caatatttct gcggtacgaa ggatcacatt gttttcaccc
    12421 gtataagaaa tcagaatccc gcacatatca ctcgtgcaat tgtacgcttc atagaatgat
    12481 tcaccaaaag tggtgacaat cgtgacatcc attttgatgc gcttcatttt cagcatgaaa
    12541 tcttgagaaa tcaaagtatt cgagttactg gcgaaaatca cgattttttt tgcttgaagc
    12601 attagttgtt tggctttttg cagttggtca tgatgaatga gagaaagtgt atcatgaatc
    12661 gtcatctgtt ctaaagaagc cattttattt gcgatcgtca taataccgtc tgtggtggaa
    12721 aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt ttcagctaaa
    12781 aaagcattac gaaaactgac ccagccatca aaacccattt tttttgctac acggatgagt
    12841 gtagaggggt ggacataaca tcgctcagca atcgcttgga tggtcatttc gccaagtgct
    12901 ggagaatcgc ttaagagaaa ctggatcact tcattttctg aaggagaaaa ggagatttct
    12961 ttgatttttt cacgtaataa catgtagata ccttcttctt atttttttac gtaaattata
    13021 acttttttga aaatgattt
//
LOCUS       DAZKAS010000075        13030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000075 DAZKAS010000000
VERSION     DAZKAS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.077"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..787)
                     /locus_tag="ACG8H7_001769"
     CDS             complement(125..787)
                     /locus_tag="ACG8H7_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP8983856.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(797..1612)
                     /locus_tag="ACG8H7_001770"
     CDS             complement(797..1612)
                     /locus_tag="ACG8H7_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8983857.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1873..2316)
                     /locus_tag="ACG8H7_001771"
     CDS             complement(1873..2316)
                     /locus_tag="ACG8H7_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFP8983858.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2450..2788)
                     /locus_tag="ACG8H7_001772"
                     /pseudo
     CDS             complement(2450..2788)
                     /locus_tag="ACG8H7_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(2776..3153)
                     /locus_tag="ACG8H7_001773"
     CDS             complement(2776..3153)
                     /locus_tag="ACG8H7_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8983859.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3377..4570
                     /locus_tag="ACG8H7_001774"
     CDS             3377..4570
                     /locus_tag="ACG8H7_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8983860.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4736..5158)
                     /locus_tag="ACG8H7_001775"
     CDS             complement(4736..5158)
                     /locus_tag="ACG8H7_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983861.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            5528..6235
                     /locus_tag="ACG8H7_001776"
     CDS             5528..6235
                     /locus_tag="ACG8H7_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983862.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            complement(6397..7569)
                     /locus_tag="ACG8H7_001777"
     CDS             complement(6397..7569)
                     /locus_tag="ACG8H7_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8983863.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7775..8590)
                     /locus_tag="ACG8H7_001778"
     CDS             complement(7775..8590)
                     /locus_tag="ACG8H7_001778"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP8983864.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8740..9186)
                     /locus_tag="ACG8H7_001779"
     CDS             complement(8740..9186)
                     /locus_tag="ACG8H7_001779"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFP8983865.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(9183..9698)
                     /locus_tag="ACG8H7_001780"
     CDS             complement(9183..9698)
                     /locus_tag="ACG8H7_001780"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFP8983866.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9771..10685)
                     /locus_tag="ACG8H7_001781"
                     /pseudo
     CDS             complement(9771..10685)
                     /locus_tag="ACG8H7_001781"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10682..13009)
                     /locus_tag="ACG8H7_001782"
     CDS             complement(10682..13009)
                     /locus_tag="ACG8H7_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFP8983867.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3753 a         2534 c         2358 g         4385 t
ORIGIN      
        1 catacaaaag tatagcacgt tttatttcat ttgtttagcc atatgtttca aaaaatgaaa
       61 aagaatgaca gtttttacat aaaaaaaact gtcattctta attgaaagtt tcaatttata
      121 aagtctacag atccatagtt ctcatcaatt tacgattgtt gaagtaaatc aaaccaaata
      181 cgatagaagg acctagtaga atacaccatt ggggctttac gaaaaagaag agaaacaaac
      241 ttgtcaatag taaaaaatct agtaaactca cataccattt tttcaacgta tagtagaacg
      301 ctatccgtag aacttgattt ggtttcgacg ctggatttct cacctgaaaa taccagcagt
      361 tggtgctaaa ggaaactgat aaacaagcaa gtaaaacaaa caacggaatc aaccattttc
      421 cataagaaaa acgtataaaa aagaaacaat caaaaatcat tatcacacta actatccaag
      481 caaatagcca ataaaaaaat ccctttagcc aaaattggcg aaacatcttt atgaacaaac
      541 gtactggtga aaaactattt tcttttgcta attcttctgt catacctatc atcacgatgc
      601 aacctggacc aataaaaagt aaagaaaaaa taaaggaaaa gacattcctt ccatctatcg
      661 acaaaaggaa cgcagctgct atatacaaga tattgacaca ccaaaaacaa aggctacttg
      721 ccaatgattg atacaaccaa taaaagaatt ccatataaac atttttgtca aaacgctgct
      781 ctttcataat cacctcctat tccttcattc ctgatgaagc gatcccttct acaaaatatt
      841 tttgcatcgc taaaaatata aaagcaacag gaagaacaga aacaactgag gcagccatga
      901 ttaatgcgta ctctgcatca aatagcccaa taaacatttt caaccctaac tggattgttt
      961 tattttccgt agaagacaaa taaatgaatg gccccatata atcgttccat gtattaacga
     1021 atgtgatgat cgtcaaactg gcaatcgccg gttttgtaag aggtaaaatg atcttgcgat
     1081 agattcccca ctctgataag ccatcgatcc gcgcactctc acataaagaa tctgggatac
     1141 tactgtaata ttgcttcaac aagaacacac caaaagcatt gaacgcttgt aatagcacca
     1201 acgaccacaa cgtatctgtt aaacccactt ttctcatcat aatgaactgc gggatcatat
     1261 aagactgcca agggacagca atcgtgccga tatatgctaa aaataaaata tcgcgccctt
     1321 taaaatttag tttagaaaac ccgtaagcag caaaactaga agtaaacaac tgaatcaacg
     1381 tgatcacaac gcttaaaaaa gctgtattct taaaaaatgt cactaaagga atacgagacc
     1441 aaataaccag ataattctct aaatgccaag ttttagggat ccattgaatc ggaatcgtga
     1501 acacttcatt atttgctttg aatgaagcag aaaccatcca taaaaaagga atgatcgtca
     1561 ataaagctaa tacaatcagc agaatcatac ccaaccattg aataaagggc atgttttttg
     1621 aacgaatttt tgcttttttt ctttcatttc gaacacctcc aaagttataa tcttcatttc
     1681 tttgtaatga ttacaatcca gaacaacggt tattatagct gattcatttc cgttcttctt
     1741 atttttgtaa gcgcttattt aacaagttat gtaaatctct ttattttttt gatttaaaaa
     1801 tgaaaaaata caaaagcaag actgtcatac gtctcgcttt tgtattgaaa tttcttcgat
     1861 tagagatact tattactgtt caaaagcttc tttgaaacgg tgaatcaatt cattgactac
     1921 aggtgataaa atccgattct ttttccagac tagcatgcag ttcgtttcaa tcattgggtc
     1981 aagcggaacg aatgtcactc gctttgaatg atgacttaca gcaccttcta tgactaaagc
     2041 agcacctacc tgattctcaa ttagcgcaaa tacattgaaa atcaaattgt acgttcctgt
     2101 gatcagcaac tgctcctttt ttatgcctaa ccagtcagca aacattcgct gtatttctgg
     2161 acgctttgag atcaaaagag gtacattcgc aaagtcttct tttgttatca cgtctttttt
     2221 tgctaaaaca gaattttcta aaatgagcaa tccccatttt tcctttcttg gtaacaaaat
     2281 cgtttcgtat ttctctgtat ctactggatc tagcaaaatc gctaaatcta acaggctttt
     2341 ttctagtcac tccgtgatat catcacttgt tccgctgtgg atatgaaatt gaacgctggg
     2401 ataatcacgg atcaactttt ctagtaatag tgccaatgta ttcgaactat cagcttccac
     2461 acagccgatc gtaaatcggc cgctgaataa ttgttttttt ctgttctcga attcttgagt
     2521 ggtttgattc gttaaatcta aaatttcttc tgctcggctt ttttagaaaa taacctgctt
     2581 ctgtcaagat cattttgcgg ttttcgcgta caaacagttc tgttcctagt tactcttcta
     2641 actccttcag ttgtcggctt aaggttggtt gtgtaagatg tagaacttct gcggcttgag
     2701 agattgttcc ttcttctgca attgtccaaa aatattttag taagcgtaac tccatagcga
     2761 tgctccttga tttttttact tgatacattc cattttattt ctagataaca cacgatcata
     2821 tttcttgatt tttccttgta aacgttccaa acattcttgc atttcagcaa tttttttcgt
     2881 caaaagttca ctttcttcaa tcaaaagctt acgccgctca aatacagttg cttctccttc
     2941 gctagatagg cgaatatatt caatcaaaga ctcgatcgat aatccggcat ttcgcataca
     3001 gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa tcacggattc cgccttttgt
     3061 ccgattaact ggtggaatca acccgattcg ttcataatag cgcaatgtat cggccgaaat
     3121 gcccttttct tcactgactt gtttgatatt catctgatca ctctttctct taatttcttg
     3181 ccactttact tatgtatttt atttattcta caactttaag tgtactccaa gtcaatactc
     3241 atttctatta aaactaagct ggattatcta tatcggttgt ttgtgtatta tttcaaataa
     3301 aaaagtcttg tttttttatt cttttttgga tatactttta acaatttagg gaaattttag
     3361 gaaaaggggt ataaaaatga caacgtatca gcgaaaggta ttagtatcga catcggcggg
     3421 aattgcttta gagaatatgg atattatgtt tttagctttt tcattatctt caatgattac
     3481 ttcttttcat attagtggta cgcaagcggg tttcatttca acgatcacta atttggggat
     3541 gctcattgga ggtatttttt ttggcatcat agctgataaa tatggacgtg tgaaagtatt
     3601 ttctcaaacg gtccttctgt tttcgattgc ttctttattg atgtattttg cttcaaacat
     3661 ctacttggtt tatctctttc gctttatcgc aggtgtagga gcgggaggag aatatggggc
     3721 gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa attggacgag cttcttcggt
     3781 tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg attttagctg ctgtgatcat
     3841 tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta ctgccggtgt tgatggtctt
     3901 gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa tctgtacaat ctgccgaaaa
     3961 gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt gcttggcaaa catgtggcct
     4021 tagtatcatg gtaactgtcc aaattgccgg ctattttggg ttaatgaatt ggctaccttc
     4081 aattatgcaa aatcgattag ggctttcggt ttctgggtct tcattgtgga tggtcagtac
     4141 tattttaggc atgtctttgg ggatgatgac ttttggcata atcatggata aattggggcc
     4201 taaactagcc ttttctttgt ttttaatttg ttcatcctta tctgtctatc tacttgtgtt
     4261 agcccatagc cagtggagtt tggtagcagc cgctgtgata gtcggctact ttatcaatgg
     4321 catgtatggt ggatatggcg cagtgattag tagtttgtat ccaacagaaa tccgcgcgac
     4381 ggcgaataat ttcgttatga accttggacg agctgttggt ggtttttctt ctatcgtcat
     4441 cggtttcttg atggatcatt atagtttaac tgccgtaatc ttgtttttga gcggtattta
     4501 cctagtcagt ctgctggtct tgacaactct tactggtgtt acagcattaa gggaaagctt
     4561 gcagcattga tagttgagtc tgtaaatgac aagaaagagg tgtggaaaaa gcttgcatct
     4621 gcggtaataa gaccagccaa atcaaaaaca tgagacactg tttccgtttg gctggtcgta
     4681 ttacttgatg cagggcagct ttttcaactg ctaatttcct tctatctggc ggcgactatt
     4741 ctttgacgtc cgtcatttca aagtacttta ctaataactt agcataacga aaaactgtgg
     4801 atacactcgt atattgagcg tctgcataat cttccaaatt tttctcagga tgttgataca
     4861 tgaattcaat caaatgataa ttatatgatt ttctgtagaa atgaagataa ataatgtaga
     4921 ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt attctcattc ttcaaaatga
     4981 ccttatcagg atattgttgg aacaattctt ctaaatctgt tagataattc aagatagact
     5041 tacttgatac cttcaattcg ttttgtagtt cttctattga acaccaacgt tctgtttcaa
     5101 gcagtaaatg aatcatttta attttctttt gagaaacttt tgttaaaaaa gtctgcatat
     5161 tttctcctcc tgtatctact ttactatact acatacaaga aaaaaacgga tacaaatatc
     5221 atattgtcac accgtcaata gtgcataaac ctgttattat ttctcttttt ttcctaagta
     5281 ttctattgat atgaatcatt taatcgttta ttatataaat gtcaataatc taataataaa
     5341 ataacgtaca tattttatta ttgcatactt aataacagaa ctatagcaaa aaatcccgtg
     5401 tatcaacacg ggattagatt aagtttcctc aatcactatt cataacacaa ttattataac
     5461 aagtaagtaa acaattgaaa aggacattta ttgaaaacgg ttgtttttta cttaaggagg
     5521 aaatttagtg aaaaaattag ttgcgttgtt tttggtcgca ggaagtttgt tgagtgggtg
     5581 tggtgctact acgtctacta ctcaaagcac aagttcagct caggcttcca gcagcagtac
     5641 cacgatctcc gaaagtacgg cagattcatc gaaagaacaa attgaaaaga aaaaagaaga
     5701 agagaaacaa aaagcggaaa aagctgcact agaaaaaaaa gtgacagaag cggaatctgc
     5761 tttgtcacaa gctgaagcaa atcctacaaa agaaaactat gcaactgctt cagcagctat
     5821 ccaagcgcta cctgaaaaca aacaagagtt gacaagccgt cttgctgcaa ttgattccac
     5881 aatcaaagca aaagaagcag aagaagaacg gaaaaagcaa gaagaaattg cacgacaaca
     5941 agcagaacaa gcgcaacaag aagcaatcgc acagcaacaa gcagaacagg cgaggcaaga
     6001 agcaatcgca cagcaacaag cagaacaggc gcgacagcaa gcagcagctg aacaggcttc
     6061 tcagcaggca caagccgcag ctcctgagca aaataatgaa caaactgtct atgtcacacc
     6121 gaccggttca aaataccata cacataaatg tggaaatggt acgtataccc ctgccacttt
     6181 aagcgaagct caagctcgtg gtttaacccc ttgctccaaa tgttatggaa actaattcaa
     6241 aagttatact ataataataa aattcttctg aaacataaaa aaatagaagc tggcgttcca
     6301 gatagtaaac caaaaatagc agtttacacg tccatttcgt cagcttctct cttttttcat
     6361 ttagtctgtt ccctctgctt ttttggacat cttcattcat tctttctcaa tcaaatagta
     6421 cacctctttt tcttttcgca gtttatatct tccttttttc aatttcattg aaaactgttt
     6481 cgaatgttta tgttttacat ggattttaag caggagctcc tcatgttgca agcgatagtt
     6541 cctgcctttt aaatcagaaa atctcactag tccgtattca tcagaagctt tcacaaatgg
     6601 aactttttct ctagtaatat agtgtttgcc gttgagcgtt tttacagaaa agaccattcc
     6661 ttcgattggt cgtccgtttg tgttctttac ataaaaagat aaaggatagt ttcgcttagc
     6721 gatggccaaa gaatctaaat aagcgatgag tagccaaact aagccgattg atccaagtag
     6781 tataccgata aaccaaataa aaaatttcca ttttttccat tgatcaattc cttggatcgc
     6841 tttttttgcc tcttttgctt gataaggaat acgatgcccc gtcacaagca gtcgatgtgt
     6901 attgaccata taaggagtgc aggtaagcaa agtcacgaga tctctgcctt tttggatacc
     6961 caaagaatct acttcgtcag gtaagaccac ttggattttt tctacttgat acgccaatgt
     7021 ctttccattg atctggatga aaaactggtc tccttttttc aacttaggca aatctgtaaa
     7081 taattttgct tctgggagtc cacgatgccc agaaagaacg gcatgggtgt ttttcccgcc
     7141 agtcggataa gaacttcctt ccaataatga cgttcctttt tgcaaaaata tttccgttgt
     7201 ctgatcgaat ataggtaagc tgacattgat ttttgggatt tcgatgactc caagcatatg
     7261 ttccataaaa aaggcattac taggtaaatc ttttaatgct tttgcatcaa ctgcttggtt
     7321 gaaactgcct atccctggtg atacgttttg ctctgccaac tgttggtttt tctttgtcat
     7381 tttttcctgc tgttttttta tttcctctga attcttattc gatgcttgct tttgataatg
     7441 gatgatcatt tgctgggcaa gaaaatcatt cagtgaatct tctacaaaag gataagaaaa
     7501 gatcccgatc ccaacgacta aaatgattgc catgaagatg tccagtattc ttattctgct
     7561 tttattcatc atgtccacct attttctaat atgtatatga agaggttgtg aaatcagcgc
     7621 cttgacctga gtattagcga agcaaacggc aaaatagttc ctcctatttt gccgtttgtt
     7681 gagcgaatac caagagaccg accacttgtc acaacctttc tccccatttg aatcactatc
     7741 gtttatttat cgtatgattt ttgtttccaa tagtttaagc ttctgtttct tgatggcgtt
     7801 ttttgaaata gatcactgct cctccaacta aagcagtacc taccaagatg aatacgatga
     7861 tccccatacc accagttgaa ggcaagattc ctttaggttg gttatcgatc gttgtttcat
     7921 cagaagttgt tggatcatca tctactgtaa attcagtgta cgcagcttca ttcttaggta
     7981 atgcgtagcc ttctggtgct tttgtttctt gaaggcagta tgccccttgt ttcagccctt
     8041 cgattgaaaa tttcccttct gaatcagaaa tcaatttcac ttcttcccag tcagaacctg
     8101 tgacttcatc tgaccaagca tatgggtatt ctcctgaggc aaggtttgct atttttttca
     8161 ttaatttaac agttggatct tttgctctta gaatagaaaa ttctgcatcg gctaattttt
     8221 cttgcgtgtt cacatcttgt ttgatgaagt ggaaatcacc gtagttttgg acattttcta
     8281 cagtaatcgt gttatccgta gttgtatttt cccgattgac tacaccgttt ggattcccaa
     8341 gattggcttc atctgcgcca tggatgatac cggttgtttg ttgcaatttt ccgtttttta
     8401 ctataaaagt agtttctttt tgtggattgg ttaattgata accatctggt gcttttgttt
     8461 caactaaagt ataattgccg tctattaatc cattgacact gatggatcct tcagtatcag
     8521 ttgtgaatgt caacgctgac tcagcaggat catagagagc gacaccagtg ttttcatcaa
     8581 aaccggtcac gaattgattc tctgaatttt tgatctggaa ttgcgcccct gctaacttat
     8641 ctacccctga aaagttccca gctttctgga ttttgatatt gctttctttg atgacatttt
     8701 tagcatatag gtgaatcgtg tctaaaggtt tgtcagggtc tagggtaagc ttaacacgat
     8761 atttggcgac gtttcgacac cgggctttgg tgtttctaca aagacgtata cagcatcttt
     8821 cccatcagat ttacctggaa gagtcaggtt agctaatcct tcaccagaag tcgttacact
     8881 tgcgaagggt gtagcgtctg ctggaactgc tgtgtcttta cttggatagt acgtagcagt
     8941 gatgtcatat acgttgaatt caacgccgtt caacggttcg ctattcggaa atgtcaattc
     9001 cgttccgttg tttatttgat tttctggtgc tgaagtgaat ttcaatttat gcaaattaac
     9061 cgtaacattt tctggtgtag gtgatacggc tgcagaaacc tctgcagttc ctaggccgag
     9121 aacgaaagga agcagcatga tagcagttcc taaaaacgtc catatttttg ttttgatgtt
     9181 tttcatttta tttccctcct attgtttcct ttttgttgtt ttttataaaa ccaagctgtc
     9241 cctgttgaaa ggactagcaa taaccctaaa attgagaaac ctccaagcat ctcttcgctt
     9301 gtttttggaa agcttcctcc atttttttca tttgatggac catttgtctg catctgaact
     9361 ggcgtttttc cattattcgt cggatttgct tttggttcgt tgatgatttc caatggtata
     9421 ggatgaagcg gatcataact gcctttttca acttcaaaga gaatggttga ttgactcaat
     9481 atatatcctt taggcggttt aatctccact agttcgtaac ttccaaaaga caatccatgg
     9541 acagaaaatg ttccatcttt actggaagta aaaatcgttg ctcttgaatc atctttggct
     9601 acccaatgat ttatcccgtt ttcttgcgct aagtattggt catttttttt gactagaaat
     9661 tgagcatctg ctagtttttg tttcgtttgc cgatccactt tgacaaattg tttgcctcct
     9721 gtttggatgg caagatgtgt ttctactgtt tgattgtcag tcgtcaaacg tacatcgtta
     9781 tcaaatgtcg ttttttcact gttttctttc aatgtcattt ggtaacggat cgttaatttt
     9841 tttccagcaa acgaactaag atcttttcca gaatctgata agacaaaacc atgttttatt
     9901 ttttgtgtgt gaaatgtatg aatcttgtct ccaccaatca aaaaatcgat cgtttctggt
     9961 tctagccata actgaggatc agctgtatct ttgatcgtat aggttcctat ctcgttgatg
    10021 ttctcaggaa ttgttacggt gatgagataa gggactttat ccccatctgc aaaatctgtc
    10081 tgcttattct ctactgtttt tgttaatgtg agtgcagcag gtattttttg ttcgctcttt
    10141 ggatacaagt ggatggtcgt caatttatga ttagatgaat ctgtcacagg aagcacaacg
    10201 acaaatggtg ctgatttttc ttttacttaa tctgaagtct ttatttccgt aaatcggtaa
    10261 acagcgtcac gaccttgctc atctttgtcc gatgcactaa aagaagcaat gccctcttcc
    10321 tcgtctactg tttttgtcac attttctgcc agtatttttt cactatcact tttttgtgct
    10381 agttttcttt gtgcgtcttc cacactactt ccttctgacc gcagcttgta aaattcatcc
    10441 gagacatcat atatagcgaa agtaacgccg ttcaaccctt ttactccttg aaagttatcc
    10501 gggatattta ttccgtcgtt ttgactgata cttggcatct tatcgctttc gaacattcga
    10561 ttatgcaaag taaatgtaat aggtgattct ctagctgaca cagtcggaga acctataaaa
    10621 aagaccagta ggataaaaaa gagaacagca aatatatcca cacgtacatt ttttgttttt
    10681 ttcatcgctg ttcacctgct tttttcttac gatggataaa ataaaagaag ccagaaaaaa
    10741 taaagatcag tcctaaaaag acaaagggca gtagacctat cccacctgtc gaaggaagca
    10801 agagagtcgg attgatcgtc aacttagcgg tatctgttgc gataatcgta taattattgt
    10861 ttacacttcc ttcgcctaaa ctaatcgtta ctatttgtga tccaatatct gtccggctgt
    10921 taactgttag ggtataatcc aatgattcgc ctaagacaac accggtaacg tggagatttc
    10981 caaccgaaat cgagtgactt tgggcatcat aattaaaggc agcgtcatcg acagtaatca
    11041 ctgcgttagc tttatagatg gtataagttc ctgatgtgtt ggctatatca gtaaatgaca
    11101 acttgggatt tgctgctttg actttgtcta ttccgctatt cgttagttta attgtatagc
    11161 tgccagcatt aaccatattt tttgctgatt gtgcatctgc tgtgtaatcc atactgctaa
    11221 gtgccacttg tactggagta agcgaactag tgacaccctc tgctagcttg acatggattg
    11281 acgggacacc tgcttttcca tcatatgtct tactgccact gagctctacc gcaccgctga
    11341 tttgcttaat cgtatagaca tcagatgtct gcacgatagt aaataaatag tttgttctgg
    11401 cagctagttt agctagtccg ctttttgtca attgccaaga cgtataggtt ccaatttcag
    11461 taggcgcagt tacttcaact cccttttcat tcatcgcttt gaaatcagtt ggattccatg
    11521 tgatttgatc aatactgaga tcattgttta aaagggatgt tgattctgtt ttatcattaa
    11581 aggcatattt aacgatcggt acaacagaaa gaccatcata gtacttactg ccatttgcat
    11641 tgattgatac tgttgcattt gtcttataat ataggatgac ttcagaaatc tgttttccat
    11701 ttgcgtcctt tacgattaca tttccatttg tatctaaact agctaaaaca ctcattgctg
    11761 attggttctt tgtttctatc gctgaaacag ctgtaaaggt atatccgttg atagtaggcg
    11821 tattagtcgt caggtcttgt gcagcacttc cgtaagtaag attcattatt ttagaaggag
    11881 caatcatctg tccaaaatag tcttgatact taatagtgat tggtgtttgt ttgcctgtat
    11941 aggtcaccgt atcgacgtta ctttctaggg ctgtgaaaga accgtttgtt tgtgcaaaag
    12001 aatagcccgt tactgctgtt ggagtgataa ccactttgtc acccacatgg attccagata
    12061 atgtagtccc gtacgttccg atttttgctg ctccaccatt gactgtataa gaaaaagttc
    12121 ctgtccgatt cgcattgacc tgaaccgttg ctgcttgata ggttggaaaa tttgaaacat
    12181 ttcccttacc atcaacgatc tgcccatttt tgatcgagta ccccgtgaat ggaatatcat
    12241 ttgctaactg tataccgatt gaaaaagtac tcatacctcc tccggcagga agccctcccc
    12301 atgttgaggg tgtaaaagaa taacttttat ccgcattggg tgtaactgaa tcgatcgtat
    12361 ttccagcggt agccaccccc atatagatac tttttgggaa tgtgatagta gtaccattta
    12421 ctgtggagcc aataaacaga ttatggatat ctggacggtt atcaataaaa gcgattgctt
    12481 tggttacgcc ttgtaacgca gttttatccg cactcatcga tccgttaacc gcaacaatga
    12541 ctccatcttt ggcatatatc actgtatatc catctggtat ggtatgtccg ttgaaattcg
    12601 cagtggccgc aacagtacta aatgtctggc ttctacctgt ttctgaataa gctttctccc
    12661 ctgctgcttt tgctgctgtg atttcttttt ctgtcggatc aatcagttga gtcgaggatg
    12721 tggttgaggt tgtctcgttg ttgctagact gattggtgct ttctatggac tgtatttcag
    12781 aatagggtgg actatttgtc tcctctgtcg tttggttagc aaaatttgac gtttctgtat
    12841 aaacgtttac ataaatactt gataagctcc ctaagaaagt aaaaatcaag atgatcgaaa
    12901 tcataatggg atgtaagtat tttcttttag atacagcttt gagtctagaa acaaaatgtt
    12961 tttgacatat acgttggatt ttattttgag ttttcttaat cattttcatc cctcctctca
    13021 ataaaaaatc
//
LOCUS       DAZKAS010000076        12805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000076 DAZKAS010000000
VERSION     DAZKAS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.078"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..545
                     /locus_tag="ACG8H7_001783"
     CDS             27..545
                     /locus_tag="ACG8H7_001783"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP8983868.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            673..1356
                     /locus_tag="ACG8H7_001784"
     CDS             673..1356
                     /locus_tag="ACG8H7_001784"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFP8983869.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1665..1738
                     /locus_tag="ACG8H7_001785"
     tRNA            1665..1738
                     /locus_tag="ACG8H7_001785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1699..1701,aa:Arg,seq:tct)
     gene            1897..2256
                     /locus_tag="ACG8H7_001786"
     CDS             1897..2256
                     /locus_tag="ACG8H7_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFP8983870.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2379..4010
                     /locus_tag="ACG8H7_001787"
     CDS             2379..4010
                     /locus_tag="ACG8H7_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFP8983871.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4072..4713)
                     /locus_tag="ACG8H7_001788"
     CDS             complement(4072..4713)
                     /locus_tag="ACG8H7_001788"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP8983872.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4899..4975
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4995..5303
                     /gene="rplU"
                     /locus_tag="ACG8H7_001789"
     CDS             4995..5303
                     /gene="rplU"
                     /locus_tag="ACG8H7_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP8983873.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5330..5674
                     /locus_tag="ACG8H7_001790"
     CDS             5330..5674
                     /locus_tag="ACG8H7_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFP8983874.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5687..5980
                     /gene="rpmA"
                     /locus_tag="ACG8H7_001791"
     CDS             5687..5980
                     /gene="rpmA"
                     /locus_tag="ACG8H7_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP8983875.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6414..9221
                     /locus_tag="ACG8H7_001792"
     CDS             6414..9221
                     /locus_tag="ACG8H7_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8983876.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9412..9903
                     /locus_tag="ACG8H7_001793"
     CDS             9412..9903
                     /locus_tag="ACG8H7_001793"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP8983877.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10006..10995
                     /locus_tag="ACG8H7_001794"
     CDS             10006..10995
                     /locus_tag="ACG8H7_001794"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFP8983878.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11028..11831
                     /locus_tag="ACG8H7_001795"
     CDS             11028..11831
                     /locus_tag="ACG8H7_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP8983879.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11852..12766
                     /locus_tag="ACG8H7_001796"
     CDS             11852..12766
                     /locus_tag="ACG8H7_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8983880.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
BASE COUNT         4065 a         2153 c         2780 g         3807 t
ORIGIN      
        1 ttgaataaag aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt
       61 ctataacgtt gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt
      121 ttggtttgac tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa
      181 atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat
      241 attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc
      301 acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
      361 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
      421 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
      481 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
      541 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
      601 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
      661 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
      721 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
      781 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
      841 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
      901 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
      961 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
     1021 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
     1081 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
     1141 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
     1201 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
     1261 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
     1321 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
     1381 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
     1441 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
     1501 gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt
     1561 tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag
     1621 aagcaattta attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc
     1681 tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct
     1741 aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg
     1801 tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc
     1861 aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta
     1921 gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat
     1981 tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt
     2041 caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg actactttat
     2101 ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt
     2161 tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg
     2221 ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc
     2281 taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat
     2341 ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct
     2401 cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg
     2461 ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag
     2521 ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat
     2581 caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta
     2641 ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt
     2701 tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa
     2761 ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat
     2821 ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag
     2881 gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta
     2941 tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa
     3001 cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat
     3061 tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg
     3121 cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt
     3181 cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa
     3241 ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta
     3301 caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg
     3361 tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac
     3421 ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc
     3481 tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag
     3541 ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg
     3601 ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc
     3661 tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt
     3721 ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca
     3781 ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat
     3841 ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag
     3901 tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg
     3961 ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa
     4021 aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg
     4081 ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt
     4141 gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc
     4201 gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt
     4261 tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc
     4321 aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac
     4381 ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt
     4441 gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc
     4501 tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa
     4561 aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg
     4621 atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga
     4681 agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt cttttattat
     4741 aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc
     4801 agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt
     4861 gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa
     4921 caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg
     4981 aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag
     5041 tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg
     5101 acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg
     5161 caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat
     5221 acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg
     5281 ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg
     5341 aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga
     5401 tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac
     5461 tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga
     5521 aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc
     5581 tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga
     5641 atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat
     5701 gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc
     5761 agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat
     5821 tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga
     5881 cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa
     5941 aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga
     6001 ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga
     6061 ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg
     6121 atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat
     6181 aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt
     6241 ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag
     6301 gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa
     6361 tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg
     6421 tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg
     6481 aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag
     6541 tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac
     6601 tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct
     6661 ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag
     6721 ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa
     6781 gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg
     6841 cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt
     6901 tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg
     6961 caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg
     7021 gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct
     7081 tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag
     7141 caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt
     7201 ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg
     7261 agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga
     7321 tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag
     7381 gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat
     7441 cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc
     7501 aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa
     7561 ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata
     7621 atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag
     7681 aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag
     7741 accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca
     7801 tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc
     7861 aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag
     7921 gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat
     7981 atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag
     8041 aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg
     8101 gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag
     8161 ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg
     8221 atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg
     8281 gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag
     8341 acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg
     8401 tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt
     8461 ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata
     8521 agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag
     8581 aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt
     8641 cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta
     8701 ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg
     8761 atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg
     8821 aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata
     8881 acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa
     8941 tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc
     9001 acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg
     9061 aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt
     9121 tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag
     9181 tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac
     9241 ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc
     9301 gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca
     9361 cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt
     9421 cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag
     9481 caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga
     9541 aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag
     9601 aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc
     9661 accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat
     9721 atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat
     9781 caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga
     9841 aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg
     9901 tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta
     9961 atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc
    10021 ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc
    10081 gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga
    10141 gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt
    10201 gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat
    10261 aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc
    10321 ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt
    10381 gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac
    10441 gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat
    10501 gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa
    10561 gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt
    10621 aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc
    10681 aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt
    10741 gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat
    10801 gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga
    10861 caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt
    10921 aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa
    10981 ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt
    11041 caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat
    11101 ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg
    11161 aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg
    11221 gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt
    11281 taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg
    11341 ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga
    11401 tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg
    11461 tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac
    11521 aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg
    11581 gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag
    11641 aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga
    11701 ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg
    11761 gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga
    11821 atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa
    11881 aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga
    11941 acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa
    12001 aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc
    12061 atacattgct tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg
    12121 cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc
    12181 tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc
    12241 ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat
    12301 tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat
    12361 tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg
    12421 gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt
    12481 atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg
    12541 tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa
    12601 ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt
    12661 aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg
    12721 tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata
    12781 gctcgggcgt tgtcccgagc ttttt
//
LOCUS       DAZKAS010000077        12755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000077 DAZKAS010000000
VERSION     DAZKAS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.079"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(330..692)
                     /locus_tag="ACG8H7_001797"
     CDS             complement(330..692)
                     /locus_tag="ACG8H7_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983881.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="ACG8H7_001798"
     CDS             complement(766..1146)
                     /locus_tag="ACG8H7_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFP8983882.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1228..2403
                     /locus_tag="ACG8H7_001799"
     CDS             1228..2403
                     /locus_tag="ACG8H7_001799"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP8983883.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2480..3064)
                     /locus_tag="ACG8H7_001800"
     CDS             complement(2480..3064)
                     /locus_tag="ACG8H7_001800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8983884.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="ACG8H7_001801"
     CDS             3265..4263
                     /locus_tag="ACG8H7_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP8983885.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="ACG8H7_001802"
     CDS             4375..4884
                     /locus_tag="ACG8H7_001802"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP8983886.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="ACG8H7_001803"
     CDS             complement(4914..5573)
                     /locus_tag="ACG8H7_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFP8983887.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="ACG8H7_001804"
     CDS             complement(5575..6339)
                     /locus_tag="ACG8H7_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFP8983888.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="ACG8H7_001805"
     CDS             complement(6347..7024)
                     /locus_tag="ACG8H7_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFP8983889.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="ACG8H7_001806"
     CDS             complement(7061..8446)
                     /locus_tag="ACG8H7_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFP8983890.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="ACG8H7_001807"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8H7_001807"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="ACG8H7_001808"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="ACG8H7_001808"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP8983891.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="ACG8H7_001809"
     CDS             9385..10242
                     /locus_tag="ACG8H7_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8983892.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="ACG8H7_001810"
     CDS             complement(10323..10658)
                     /locus_tag="ACG8H7_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983893.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="ACG8H7_001811"
     CDS             complement(10725..12095)
                     /locus_tag="ACG8H7_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFP8983894.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..12709)
                     /locus_tag="ACG8H7_001812"
     CDS             complement(12173..12709)
                     /locus_tag="ACG8H7_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8983895.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3795 a         2588 c         2124 g         4248 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattct ttactataat ttgttactct ccccatcatt cttacctccg
    12721 tctcttttca tctacccata aataaataac ataaa
//
LOCUS       DAZKAS010000078        12386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000078 DAZKAS010000000
VERSION     DAZKAS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.080"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG8H7_001813"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG8H7_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP8983896.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG8H7_001814"
     CDS             complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG8H7_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP8983897.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="ACG8H7_001815"
     CDS             complement(3218..5533)
                     /locus_tag="ACG8H7_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP8983898.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="ACG8H7_001816"
     CDS             complement(5718..6578)
                     /locus_tag="ACG8H7_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFP8983899.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="ACG8H7_001817"
                     /pseudo
     CDS             complement(6637..7269)
                     /locus_tag="ACG8H7_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7436..8197
                     /locus_tag="ACG8H7_001818"
     CDS             7436..8197
                     /locus_tag="ACG8H7_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8983900.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="ACG8H7_001819"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="ACG8H7_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFP8983901.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG8H7_001820"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG8H7_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP8983902.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         3496 a         2628 c         2194 g         4068 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacc
//
LOCUS       DAZKAS010000079        12132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000079 DAZKAS010000000
VERSION     DAZKAS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.081"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..572)
                     /gene="frr"
                     /locus_tag="ACG8H7_001821"
     CDS             complement(15..572)
                     /gene="frr"
                     /locus_tag="ACG8H7_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP8983903.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(575..1297)
                     /gene="pyrH"
                     /locus_tag="ACG8H7_001822"
     CDS             complement(575..1297)
                     /gene="pyrH"
                     /locus_tag="ACG8H7_001822"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP8983904.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1433..2314)
                     /gene="tsf"
                     /locus_tag="ACG8H7_001823"
     CDS             complement(1433..2314)
                     /gene="tsf"
                     /locus_tag="ACG8H7_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP8983905.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2413..3195)
                     /gene="rpsB"
                     /locus_tag="ACG8H7_001824"
     CDS             complement(2413..3195)
                     /gene="rpsB"
                     /locus_tag="ACG8H7_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP8983906.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3554..4033
                     /locus_tag="ACG8H7_001825"
     CDS             3554..4033
                     /locus_tag="ACG8H7_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP8983907.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4250..5341
                     /gene="phnW"
                     /locus_tag="ACG8H7_001826"
     CDS             4250..5341
                     /gene="phnW"
                     /locus_tag="ACG8H7_001826"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFP8983908.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5334..5462
                     /locus_tag="ACG8H7_001827"
     CDS             5334..5462
                     /locus_tag="ACG8H7_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFP8983909.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5466..6011
                     /locus_tag="ACG8H7_001828"
     CDS             5466..6011
                     /locus_tag="ACG8H7_001828"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFP8983910.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6465..8156)
                     /gene="argS"
                     /locus_tag="ACG8H7_001829"
     CDS             complement(6465..8156)
                     /gene="argS"
                     /locus_tag="ACG8H7_001829"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP8983911.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8576..9523)
                     /gene="arcC"
                     /locus_tag="ACG8H7_001830"
     CDS             complement(8576..9523)
                     /gene="arcC"
                     /locus_tag="ACG8H7_001830"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP8983912.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9638..10657)
                     /gene="argF"
                     /locus_tag="ACG8H7_001831"
     CDS             complement(9638..10657)
                     /gene="argF"
                     /locus_tag="ACG8H7_001831"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP8983913.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10748..11977)
                     /gene="arcA"
                     /locus_tag="ACG8H7_001832"
     CDS             complement(10748..11977)
                     /gene="arcA"
                     /locus_tag="ACG8H7_001832"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP8983914.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3313 a         2518 c         2193 g         4108 t
ORIGIN      
        1 tttttatgat tttattaaac ttccaataat tctttttctt tatcagcagc gatcttatct
       61 acttctttga tgctgttatc tgtcaatgtt tgtacgtctt tttctaatcc gcgcaaatca
      121 tcttcggtaa tatcaccatt tttttgttgt tttttatatt catcgatcgc atcacgacgg
      181 atgttacgaa tcgcgatttt tgcattttct gcttcttttt taacatcttt agctaattct
      241 tttctgcgtt cttctgtcag ttgcgggata actaagcgaa tgacatttcc atcattggtt
      301 ggactgattc cgatatcact tgccataata gctttttcaa catcttgaag aatgctcttg
      361 tcaaaaggtg tgatcattaa aacacgtgct tctggaattt ggattgatgc catttggttc
      421 aatggtgttg gcgcaccgta gtagttcaca gtaatacggt ccaaaaggct agcgttcgca
      481 cggcctgcgc ggatttgccc aagttcacgt tgaagatttt gtgctgcttt tttcattttt
      541 tcttttgctt ctgtcataat tgcatctgcc atttttattt cccccttaca gttgttccga
      601 tattttcgcc taagattgca cggcgaatat ttccgtgttc atttaaattg aagacaagca
      661 gtggaatatc attatccatg cttagtgaac ttgcagttga atccatgact tgtaatcctt
      721 tggcaataac ttccagatga gtcagttctt caaattttac tgcatttgca tctactttcg
      781 gatcagcaga ataaacaccg tctacattgt ttttagccat caggataaca tctgcgccga
      841 tttctgccgc gcgtaatgct gctgttgtgt ctgtcgagaa gtacgggttg ccggtaccgc
      901 ctgcaaagat aacgatccgt cctttttcta aatgacgttc tgctttacgg cgaatatatg
      961 gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg tgtcggtaca cccacgtttt
     1021 ccaacgtatc ctgtaaagct aaggcattca taactgtagc taacataccc atgtaatcag
     1081 cttgagcacg ttccatgccc atttgtgctc cgatttgtcc gcgccagata tttccgccgc
     1141 ccacaacgat tgccatttca acgcccagct cgtgaacttc tttgatttct tggatgattt
     1201 ctttgattac tggtggtttg attccaaatc ctgcgtcccc agctaaagct tctccactca
     1261 attttaagac tacacgctga tatttcggtg tgaccatgtt cattcctcca atattttcta
     1321 ttgccatttt accatactta gatgatttcg acactctaag actggttttt cttgttttgc
     1381 ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt tattcaacag aattattttt
     1441 tcacttggct cataacttct tctacaaagt tatcttcgcg tttttcgata ccttcgccaa
     1501 cttcgaaacg tacgaatgtt ttaacagttg cgccttttga tgcaacgtat ttttcaacag
     1561 tcatatcagg atctttaacg aatggttggt ccaccaaagc gatttctgct ttgaatttct
     1621 tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg tttgccttcg ttcaatgctt
     1681 gttctgtaag aacagttttt tcatgttcta attcagcttc tggaatttgt gattcgttta
     1741 cataacgagg attgatagct gctacgtgca tagctacgtc tcttgcaact gtttcgtcag
     1801 ttgttccatc tagaacagtc aatacggcaa tacgtccgcc catatgcaag tatccaccaa
     1861 atgcagcatt gtcgtctttt tctacaactt caaagcgacg gaagctgatt ttttctccga
     1921 taacttgagt tgcttcgatc aagtcagatt caattgtacc tttatctgtt ttgattttca
     1981 ttgcagcttc catgtctgct ggtttgtttt cagcaacaag ttctgcaatt tcttttacta
     2041 aatcttggaa catttcgttt ttagaaacaa agtctgtttc tgagtttact tcaacgattg
     2101 ctgcagtgtt acctttcaca gcaactgaag ccaatccttc agctgcaatg cggtcatttt
     2161 tcttcgcagc ctttgccatt cctttttcac gtaaaagatc aactgctttt tccatgtcgc
     2221 cttctacttc aactaatgct tttttcgcat ccatcattcc tacgccagtc atttcgcgta
     2281 gttcttttac cattttagct gtaacgtctg ccatgtttaa ctcctcctgt gtgtttttct
     2341 ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg cccagccttt gaaacagctc
     2401 catcattcaa aattattctg ctgaagaatt gtcgccttca acaacatcta cgatttcttc
     2461 gattgatgtt gctgtgtttt cttctgtgaa atcttcttca actacttgat cttcaccttg
     2521 gtttccttcg atgaaagcat cagccatttt tgaagtaatc aatttaacgg cacgaattgc
     2581 atcgtcgttt gaagggatta ctacgtcgat ttcatctgga tcgcagtttg tatcaaccat
     2641 agctacgatt gggatattta atttgtgtgc ttcttgaaca gcaatacgtt ctttacgtgg
     2701 gtcaacgatg tacattacat ctgggattct tggcatatca gcgataccgc ctaagaattt
     2761 ttcaagacgt tcacgttctt tgttcaaacc agcaacttct tttttaggta gaacttcaaa
     2821 agttccatct tcttccattg cattgatttg tttcaaacgg ctgatacgtt tttggatagt
     2881 atcccagttt gtcaatgttc cgcctaacca acgatggtta acgaagtatt gtcctgcacg
     2941 agttgcttct tctttgattg cttcttgtgc ttgttttttt gtacctacga ataatgctac
     3001 gccgccttct tcagcaacgc tcttcatgta atcataagct gcatctacta atttaactgt
     3061 tttttgcaag tcaatgatgt agattccgtt tctttctgtg aagatatatt tcttcatttt
     3121 tggattccag cgacgagttt ggtgaccaaa gtgtacgccg gcttctagta attgtttcat
     3181 agaaattact gccatgtttg tttcctccga tttggtttta tttttcctct tcttatcttc
     3241 taccttgccg gcaaactttt tcaagcacct accgacaatc caataagaat gtggatttgc
     3301 tgatgcataa agcaccctca gctattatac agacaaaaga tccgtatcgc aagagcaaat
     3361 atacttttct gctcaacatt caatagttta cctgaaagat aaaaagaatt caagaaaaac
     3421 agtagatttc tctttgtttc tttattatcc tttttccata aaataatttt aggtataaaa
     3481 gacgaatatt attgggcaat ccttcttttt tttgttatga tacatgtgaa atctttcgta
     3541 aaggagaaag aaaatgcgca aagctcaaag acatttgctg attaaacaag tcattgaaga
     3601 attcccgatc cgtacgcaag aagaactatt aaacaaactt gggcattatg gcgttaccgc
     3661 cacgcaggca acgatttctc gagatatccg tgatctaaaa atcgtgaagg cacctgatga
     3721 aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga actcctgaga ctaggactga
     3781 ggaagaggat cggctggttc aaatgattga agacattgta ttgaaagtcg aacgagtcca
     3841 ctttttgacg atcgtcaata ccttgccaga caatgctcat ttatttgcat ctgttttaga
     3901 tgagatcaaa cctccctcta ttgtcagtac aattgctgga ttcgatacga tcatggtcat
     3961 ctcggaaaat gaggaggacg caaaacgagt agaagaattt cttcaccatc caactgaagt
     4021 atcacttttt taatcagagc acgtattcct aatatggaat gcgtgttttt ttattttcaa
     4081 tattttcctt attgaataaa cctttacact ctttacaaaa atacatcatt ttttattcaa
     4141 atttttcaac accttgattg aatcacaata atcatcattt atattggatg ttaacaagag
     4201 cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt
     4261 attaacttct ggaccattga cgacttctga aacagtgaaa aatgctatgc tgatcgatca
     4321 ttgtacttgg gatcaagaat acaaagaaat tacacaatgg ataagaaaaa aattattgga
     4381 tcttgctcaa gtatcggaag gaatatacac aacggtctta atgcaaggta gcagctcctt
     4441 cggggtagaa tcggtattaa gttctgtgat caggccagaa gatactgtac tgatttgttc
     4501 aaacggcgca tatagacaac gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac
     4561 cgtttatgag gtatcggaac atcaaatcct ccaagctcat gtgattacac agctgattga
     4621 agagaatccg tctatctccg cactagcaat ggtccatagc gagactactt ccggtatttt
     4681 gaataatttg gaagagattg ctgctgtcac acaagaaaga gatcttttgt ttatagtgga
     4741 cgcgatgtca agctttggtg ggataccgat ttgtgtagcc gaactaggga ttgatttttt
     4801 gatttccagc tcaaacaaat gtattcaagg tgttccaggc ttttcttttg tacttgctaa
     4861 taaagcggaa ctggaaaaac gaaaaaatgt tagccgaagt ttgtcacttg atctatatga
     4921 ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt ttcacttccc ctacccatac
     4981 ggttttggct tttcaacaag cgctgaagga actagaggca gaaggaggaa tcgaaactcg
     5041 atattcacgt tatctcgcca ataatcatgt gctacgacaa aaaatgacag agatggggtt
     5101 ccagcctttc attcaagaat atcaggggcc attcatcacc tcttttcttt accctaagga
     5161 ctggcgattt gattttaatc atttttatca gtatttaaag caaaatggat ttgtgatcta
     5221 tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga aatattggcg agatctatcc
     5281 ggaagatatt ttggaactga cagcaattat caaagactat aaggagaacg ttaatgatta
     5341 aaactgtaat ttttgattgg gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc
     5401 acgcttttag aaatgctttt ctagaaaaag gaatccaact gactgacaaa gaaatccgct
     5461 aaccgatggg aaaattaaaa tgggatcata tccaacagct tctttccctc ccttctgtca
     5521 aaaaacaatg gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt
     5581 atcaacaatt tgaacattat ctgtttaaag atttggtcaa gcattctacc ttgaaacccg
     5641 acacattgga aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgaatg
     5701 gatttaatgc tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac
     5761 cagacttcat ggcaactttt gaagacgtcg aagggtatgg tcgaccgttt ccttatatga
     5821 tccataaaaa catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag
     5881 atacggtctc agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg
     5941 aaggtagttc gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa
     6001 agatcgttta aaacagtctg ttgctcttag attccaacaa gccggcgctg atatttgtgt
     6061 aaacaattta aaagagttaa cagaatacat tttgtacgct gacatgaatc cagaatgact
     6121 tttatacaaa ataagaggtt gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa
     6181 gcaaaaatag ctcctcatat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     6241 tgaacaccgt ttattcgaaa aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc
     6301 ttatttgcaa acaaacagtg tttaagagac aacctctcca ctaaacggtg ttcattcagc
     6361 tgactctcgg tatttgctca aaaaacgata aaatttgaaa agctattttc tcttttgttt
     6421 ctctaatact cggatcagaa cgctgatttc acattctctt tttcttacat cttatctggt
     6481 gcatgaagtc ctagcaaacg aaggtcttct ttcaataata cagtcactgc ataaactaga
     6541 gctaagcgtg cttctttttg ttcatcatca gctaaaatct tcgtatgagc atagtatttg
     6601 ttgaatgctt gtgctaattt gattgcatgt ttcgcaatca ctgacggttc gtatttttcc
     6661 ccagcagaca agaccgtttc agggtatttt tgaactaatt taaccacttc ccagctggta
     6721 tcgtcattca atgcatagtc tgctgtttct gaagggacaa atcctgcttt ttccaacagg
     6781 ctgactgcac gagcatgtgt atattgcaca taaggtccag tttctccttc aaatcgaaca
     6841 acttcttcca ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg
     6901 actgccccaa ccccaacttg tttagctaca gcatctttgt tttccaaatc agggtttttc
     6961 tcgctgattt gttctttagc agattggatt gcttcattta atacttcttc taataagacg
     7021 atctttcctt tacgagttga taatttttta ccaccttgcg tgatcaaacc aaatggaata
     7081 tggtgcatgt cgtcagacca gtcaaagccc atttccttca agacagcttt caactgttta
     7141 aaatgatagc tttgttcatt ccctactaca tatagagatt gtttgaagtc gtaagtacgt
     7201 ttacggtaca atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc
     7261 ttaatcaacg ctggattcag gtcataagca gataggtcaa cgatctctgc acctttatcc
     7321 tcgttcagca aatgtttttc ttctaaaagt ttgacgattt catccatctt gtcattgtag
     7381 aaagcttcac cgttcaacga atcgaaacgt acttccagca gatcatagat tttattgaat
     7441 tctttcatcg attcatcacg gaaccattgc cataattgaa tagcctcttt atcgccttct
     7501 tccagacgtt tgaaccaagc acgagcttct tcatttaatt ctggttctgt ttcagctact
     7561 tcatggaatt ggacgtataa acgaagcaat tcattgattg gttctgcttt aacagcttct
     7621 tctgtacccc attttttgta agcgacgatc agttttccaa actgtgttcc ccagtcacct
     7681 aaatggttga tgcggattgg ctgataaccg attttttcca taatgaaacc gatcgaatta
     7741 ccgataactg tcgaacgcaa atgtcccatt gagattggtt tggcaatatt aggagaagac
     7801 atgtcgattg gtactgttcc ttgattcccg atgttgctat ccccataatg ttctttttct
     7861 ttcactactg tttgcaaaac ttttttgctg ataagttctt tattcatgaa aaagttcaag
     7921 taaggtccga caacttcgat tttttcaaag ttggcagaat cgattttttc tgctaaatct
     7981 gcagcgattt gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc tggaaatgca
     8041 acatctccgt gttcagctga ttttggattt tctagtaatt gctcgacttg ttccagtgtt
     8101 aaatcatctt tgacagcgtc atagacagct tttgcgacaa gatctttatt attcatcttt
     8161 attcctccta aagtgttccc ttgattgtta aacaaaaaaa atctctagcg acggcatact
     8221 tagtatgcca tcactagaga cgagatttcc cgcggtacca ctccaattgt cctaaaaaag
     8281 gaccagctcc atctgatatc ggtagacgac cgtctgttta aaacagaatt ctccaagtgc
     8341 gcttcgttgt atcttcttca ttcggcttcc accacttccg aattcgctag ccaagaatga
     8401 tacaagtact ctcttgttca acaaatctta gtcttattaa actttcttga attatatcag
     8461 aaaaaaaaac gaaaggctag tcgtttcata aaaaaacaat ccagatggcc taaaggagaa
     8521 gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta acaaattaat
     8581 cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt tctaaagaag
     8641 tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgct tctactttcg
     8701 gaagcatgct tcctggagcg aattgctttt catcaatgta ttgtttcatt tctgaaacag
     8761 taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca ttgtctactc
     8821 ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct gaagcaaaat
     8881 ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca acaggaatcc
     8941 caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg atgacacgtg
     9001 cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca cgtccagcat
     9061 cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct tcttccgtgt
     9121 agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca tccacaataa
     9181 cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt acttcgccca
     9241 tggcgttttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat gtatctaaag
     9301 gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg ttaccgactt
     9361 gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat ttcactaaat
     9421 actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc gtagacaaga
     9481 tggcattgcc ccctaaagcg acaactactt ttcgtttggc catttcattc gtcctttctt
     9541 cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt ttccctcaat
     9601 gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg ataaacaagt
     9661 tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct gcttggtcaa
     9721 attgacgagc atatttgctg cggaagactt catctgttac ttccatttca gtgatgccaa
     9781 agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt
     9841 gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata ttgatttggt
     9901 aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt gatacccata
     9961 catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg gtaatcatca
    10021 attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct tcttcaggtt
    10081 gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct gtgactaata
    10141 aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata ccttctactg
    10201 ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga tgccattcat
    10261 cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct accatttttt
    10321 gactgaatcc acggaattcg attccgtcaa acatacgtcc tagtacttta gctgtatctt
    10381 ctgtggattc ttttttacct aattggatat catttgcacc tagatattca ggatgtgcac
    10441 ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata
    10501 aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt ttcaagtctt
    10561 tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag tctttttctg
    10621 ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc ctttagattg
    10681 tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac tgccgtcagg
    10741 aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt ggaccaccac
    10801 gacctcgtga caattcactt gaattgattt ctaacacttt gatgccatag ctacgaagca
    10861 attcgttcga tacataattt cggttataag tgactacaac acctggagcg attgccaatg
    10921 tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca ttaccagttg
    10981 ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt aagttatctg
    11041 aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc atcttaccgt
    11101 ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt gtaaatacgg
    11161 tatccaaatg catcattgca cggttgttcg gaattttgat tgccaacact ttttcaaatc
    11221 ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca ttagttcgtt
    11281 gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg ccttcgatat
    11341 gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat cttggatgat
    11401 gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat
    11461 tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac aagtttggca
    11521 ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcatataga ctgctagaac
    11581 gtacttcgat atcttttgta cgaaccccag ccatgatttt atctaccatt tcttgcgttt
    11641 ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca atatgagatt
    11701 catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc gcttctgccg
    11761 ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttc gcaaagttgt
    11821 catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga cgatccataa
    11881 tatctggtgt caaattttcc acttcttgtc caggccgttt cagtaaaact gtctttaatt
    11941 tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc cttctttttg
    12001 attacatgta taggatatcc tccttttgta atcactttca taattttgtc tcattcaaaa
    12061 tgagacattc ctcacattaa tattcgataa ttaaataatt aaacgttttt ctatttttaa
    12121 gcgtaattta tt
//
LOCUS       DAZKAS010000080        12107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000080 DAZKAS010000000
VERSION     DAZKAS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.082"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..694
                     /locus_tag="ACG8H7_001833"
     CDS             <1..694
                     /locus_tag="ACG8H7_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFP8983915.1"
                     /translation="LVTVAKRPLDRIDQEAVFCQELAIQGTLLPLTETYLKEYFPELH
                     ATEAFTVLQAFFLSRVINHLIAPIQNKIMAETIEKMKQDSKEIYAKNNSFLVASLSEL
                     KITDTYLPDIATSLTLLVRRLKERYLQDRKKIIFLLEGDLYVCQSIRSRVLHYFGKRH
                     DLFFMTIQALSDEFLAQEDIDLVVTNYSDYLSEYTVNTDYLLLKTVPDSQDWRLLFQK
                     IEPQLSTLIGLN"
     gene            complement(752..1747)
                     /locus_tag="ACG8H7_001834"
                     /pseudo
     CDS             complement(752..1747)
                     /locus_tag="ACG8H7_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558311.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1860..3062
                     /locus_tag="ACG8H7_001835"
     CDS             1860..3062
                     /locus_tag="ACG8H7_001835"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HFP8983916.1"
                     /translation="MLVKYKVNDAGSRPVKIKPRELYRASHLDSCIYRYYAECLNKDY
                     EKVAEIRRIGDNVVAYRVGKGKSNIDYAAEAIDFIKRSNDGCYIFLGDFEKFFDTLDH
                     EYLKCKLKEVLSMSTLDEDWFKIFKTITSFSWVEQKDLNDLFGSEKEQYKNGLKSYFD
                     TWEEFRNFKADGHVCKNTNSFGIPQGTPISALLSNIYMIDFDDWLNKLSSKYGGKYLR
                     YADDFILILPFSTTSKDIFLEIIREILQYASEVTKLKIERNKTEKYLFTNDSVYLFES
                     NKNSTSSLVKSKLDYLGFVFDGSQVKMRQKSVSKFYRKADKAIRAAREKSRIKKSSYL
                     IGKRHIYRYFFDVGVRDSKGSKFGNFISYARRCQMKFDQISPLTENLMMEQIKNRRKK
                     LLKKMYKN"
     gene            complement(3227..3460)
                     /locus_tag="ACG8H7_001836"
     CDS             complement(3227..3460)
                     /locus_tag="ACG8H7_001836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983917.1"
                     /translation="MSQLNQTYENYLQQPEALSFTEMTTIYQEILKNADMNDKDFIEI
                     WEELMGAAIRYTVILGHHAYSYKKNKNNVQEVM"
     gene            complement(3719..3913)
                     /locus_tag="ACG8H7_001837"
                     /pseudo
     CDS             complement(3719..3913)
                     /locus_tag="ACG8H7_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367047.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3974..5487)
                     /locus_tag="ACG8H7_001838"
     CDS             complement(join(3974..5326,5329..5487))
                     /locus_tag="ACG8H7_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8983918.1"
                     /translation="MYTYEERKKAVDLYFKYDQALAATTKKLGYPSVGALRQWIREFQ
                     SSDKLHKNIRTSWKYTLAQKEVAVNHYLDYGQCYARTIRKFGYPNKTTLRKWLEEYAP
                     STRKVRQNSIHYTKQEKEAAVLALCTRSLSAKSIANDFGVTRQALYLWKNKLLGKGAT
                     KPLKSMNVTPEIKLLKEERDILQREVYKLRLEKEILEQASDLIKKEPGIDPIILTNKE
                     KTQVIDALCSKYSVIELLRILDLPKSSYYYHRKQLAIPDKYLKERSLIVQLFHDNKGR
                     YGYRRIYCSLKLLGITLSEKVVRRIMTEEKLKVKSLRLRKYNSYGGEITPAVPNLLKR
                     DFKADQPNKKWLTDITEFHIPSGKVYLSPMIDCFDGAIVSWTISTKPNADMVNSMLEN
                     AIATLNAKDSPIVHSDRGAHYRWPGWIKRMKEACLVRSMSKKGYSPDNSACEGFFGRL
                     KNEFFYGQDWKSTSIDQFITDLDNYILWYNKKRIKCSLGGLSPLDYRKKLGIL"
     gene            complement(5810..6634)
                     /locus_tag="ACG8H7_001839"
     CDS             complement(5810..6634)
                     /locus_tag="ACG8H7_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8983919.1"
                     /translation="MEIKVLFENSRINLNFKSGYGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTEIAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            6977..7393
                     /locus_tag="ACG8H7_001840"
     CDS             6977..7393
                     /locus_tag="ACG8H7_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8983920.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            7390..7860
                     /locus_tag="ACG8H7_001841"
     CDS             7390..7860
                     /locus_tag="ACG8H7_001841"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8983921.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            7874..8647
                     /locus_tag="ACG8H7_001842"
     CDS             7874..8647
                     /locus_tag="ACG8H7_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8983922.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            8661..9473
                     /locus_tag="ACG8H7_001843"
     CDS             8661..9473
                     /locus_tag="ACG8H7_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8983923.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            9527..10492
                     /locus_tag="ACG8H7_001844"
     CDS             9527..10492
                     /locus_tag="ACG8H7_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8983924.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            10629..>12107
                     /locus_tag="ACG8H7_001845"
     CDS             10629..>12107
                     /locus_tag="ACG8H7_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8983925.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQE"
BASE COUNT         3950 a         1957 c         2274 g         3926 t
ORIGIN      
        1 tttagtaacg gttgctaagc ggccgttgga tcggattgat caggaggcag tcttctgtca
       61 ggagttggcg atccaaggga ctttacttcc attaacagaa acgtatttaa aggaatattt
      121 tccagagttg cacgcaacag aagcatttac tgtcttgcaa gcattctttc tttcccgagt
      181 aatcaatcac ctaatcgccc ctattcaaaa taagattatg gcagaaacga ttgagaaaat
      241 gaaacaagat agtaaagaaa tttatgcgaa aaataattcg tttctggttg cttctttatc
      301 ggaattaaaa ataaccgaca cttacttgcc tgatattgct acgagtttaa cgttattagt
      361 cagaagattg aaagaacgtt atctacaaga tagaaagaag attatctttc tgcttgaagg
      421 ggatttgtat gtctgtcaaa gtattcgttc aagagttctt cactacttcg gcaaacgtca
      481 tgacctattc tttatgacga ttcaagctct aagtgatgag ttcttagcgc aagaagatat
      541 tgatttagtc gtaacgaatt acagcgatta tttgtctgag tacacagtaa atacagatta
      601 tctactatta aaaaccgtac cagatagcca agattggcga ttgctattcc aaaaaattga
      661 accacaactg agtacgttga ttggtctgaa ttaacttgaa gcctatttaa cttttttcgt
      721 taagtaggct ctttttttgc gtcttgaatt gtcaaatcaa gtgcaacact ttattaaaga
      781 agacttcata aggtgttttc caacctagac actttctagg tctgctattt agtttttcaa
      841 ccatccgata aatcgttgcg tcatctaccg tgtccatctc ttttgatttc ggtaagtact
      901 ctcgaatgag tccattcgta ttttcatttg ttcctcgttg ccaaggggca tgcggatctg
      961 gaaaataaaa gggcacatta ttcaatgctt ctgtaaccgc gaaatgattg gagaattctt
     1021 ttcctctatc tggagtgatc gttttgacca catcattggg gatactgctt aaaagttcaa
     1081 tcattttttc gctcacgtaa gcagctttct ttcttggtac ctttccagct aaaaggaatc
     1141 gcgattttcg atctgtcaac gtcactaaac aagcaccacc agtttttcca gctacagtat
     1201 cggcttccca atgtccaaat tcacttctat cgtctgcaga ttttggacga tcatgaatcg
     1261 aatgcgtaat gcgtattttt ccacgagcct cgacatgatt ttttgtatgt cttgtttttc
     1321 ctcgatggcg taatgaacgg atcagccctc tattgccact cgagagcggt tctgtatcga
     1381 aaataccgtt gtaaatggca cggtagaccg tgataaaact gattgagaag tccacctgtt
     1441 caagcttcaa acgattagag atttcctctg gcgaccattg aaattctaca aataaatgac
     1501 gaacgattga ccaaattcgc ggatcattta gaatagaatg acgccctcag tcctttctac
     1561 gagaagcata ctttttgtct gctttggaag gtgagtaggt tttctccttg tttcttctca
     1621 attcacgtga aatggtagac ggactacgat gaatgtcatt gccgattttt ctaatagaaa
     1681 caccttcttc atgaagtaga aagattcttt ctcgttcgag tatggtaaga tgatggtact
     1741 ggctcataat tgtcttcctc ctagtagtgt tctcgacaaa cctattctag ttggatattg
     1801 attatgagtc attttttagg tgttgcactt aaagtgtaaa ttcaaaacac aaaaaaaagg
     1861 tgttagtaaa atataaggtt aacgacgcag ggagcaggcc tgtcaaaatc aagccgcggg
     1921 aactttacag agcttcacat ttagattctt gcatctacag atactacgca gaatgtctaa
     1981 acaaagatta tgaaaaagta gcagaaataa gaagaattgg tgataatgtt gttgcttata
     2041 gggttggaaa aggtaaatca aacatagact atgcggctga agcaatagat tttatcaaaa
     2101 gaagcaatga tggctgttat atttttttag gagattttga aaaatttttt gatactttgg
     2161 atcatgaata tttaaagtgc aaattaaaag aagtcttaag tatgagcaca ttggatgaag
     2221 attggtttaa aatttttaaa acaataacta gtttttcttg ggttgaacaa aaagatttaa
     2281 atgatttatt cggttcagaa aaagaacaat ataagaatgg tttaaaatct tattttgata
     2341 cgtgggaaga atttaggaat tttaaagcag atggtcatgt atgtaaaaat actaacagtt
     2401 ttggaattcc tcaaggaacc ccaataagtg ctttgctatc aaatatatat atgatagatt
     2461 ttgacgattg gctaaataaa ttatcatcca aatatggtgg gaaatattta agatatgcgg
     2521 atgatttcat tttgatactg ccctttagta caacctctaa ggacattttt cttgaaatta
     2581 tccgagagat tctgcaatac gcatccgagg tcactaagtt aaaaattgag agaaataaaa
     2641 cagagaagta tctttttaca aatgattcag tatatttgtt tgagtcaaat aaaaatagta
     2701 catcaagtct agtaaaatca aaattggatt acttgggatt tgtatttgat ggaagccaag
     2761 taaagatgag acaaaaaagt gtttcaaaat tttatagaaa agctgataaa gcgatcagag
     2821 ctgctagaga aaaaagtcga attaagaaat cgagttatct aattggtaaa aggcatattt
     2881 atagatattt ttttgatgtg ggtgtaaggg atagtaaagg gagtaaattt ggaaacttca
     2941 tttcctatgc tagaagatgt caaatgaaat ttgatcaaat atctcctctg acggagaatt
     3001 taatgatgga acaaataaaa aacagacgta aaaagctatt aaagaaaatg tataagaact
     3061 gataatcaaa tgatatgctc ccatcatagt agacgttaga aaaaaatatt gataatctac
     3121 tatgatgaga atttatcaaa tcgtgacttt catatctatc gtccattaac cgctgaaaca
     3181 aacaccaaat aattcgcgaa atcgccaatc cgttgtcggt ggtcattcac atcacttctt
     3241 gtacgttgtt cttgttcttc ttgtaagaat aagcgtgatg tccaagaatg actgtataac
     3301 gaatagcagc acccatcaac tcttcccaaa tttcgataaa atccttatca ttcatatcag
     3361 cattttttaa tatttcttga tagatagtgg tcatttcagt aaaagatagc gcttctggtt
     3421 gttgtaaata attttcgtat gtttgattca attgggacat taaatcactc cttgaaatgt
     3481 ttttgtttta actagtttac tacaagaaat aaaagttagt gcatcaaaag aaaaacagga
     3541 aagcatacca ttttggtagt tttttctcgt tgagttggta taaatttgat gagacaaata
     3601 ggctagtatc actttattca ttgatactag cctattttgt atccaaaatt tatgaaaatg
     3661 attaaaacaa atctttttct tgctcaatga attgagcaaa tttatcagca cttcgttctt
     3721 atcgttctgg ataaaaatag ttgtagactt gttttgtcat ttttgaatct ttatgtccaa
     3781 cacgatacat aatatcctat agtttcatgt ctgccattgc gcacaaggaa acgtgagttt
     3841 ttctaaattc atgaagagtt atccgttttt caaggtgata tttattagcg atcatgtcat
     3901 accaatcatt tggtgtcaag ggcatcttaa aattgtaggt ttgggggtgc ggacgttttc
     3961 ctgcaccaaa attttataaa atacctagtt tcttacgata atctaatgga cttaatccgc
     4021 ccaatgaaca cttgattcgc tttttgttgt accaaagaat gtagttatcc aaatcagtga
     4081 taaattgatc aatagatgtc gacttccaat cctgcccata aaagaattca ttcttgaggc
     4141 gcccaaaaaa gccttcacaa gcagaattat caggagaata tccttttttt gacattgaac
     4201 ggactaaaca ggcttctttc attcgtttga tccaacctgg ccaacggtaa tgtgcgccac
     4261 gatcagaatg aacaattgga gaatcctttg catttaatgt ggctatagcg ttctctaaca
     4321 ttgaattgac catatctgca ttgggttttg tgctaattgt ccaactaacg atagctccat
     4381 caaaacaatc aatcattgga gaaagataga ccttcccaga gggaatatga aattctgtaa
     4441 tatcagtgag ccatttcttg tttggttggt cagctttaaa atcacgtttt aaaaggttcg
     4501 gaacagcagg agtaatttct cctccataag aattgtactt tcttaatcga agtgatttca
     4561 cctttaattt ctcttcagtc ataatgcgac gtaccacttt ttcagataaa gtaattccta
     4621 ataacttaag cgaacaataa atacgacgat agccatatct tcctttattg tcatgaaata
     4681 attgcacaat taaggagcgt tcttttaaat atttgtctgg gatagctaat tgtttcctgt
     4741 gatagtaata gctacttttg ggcaaatcaa gaattcttaa tagctcaatt acgctatatt
     4801 tggagcataa ggcatcaatc acttgtgttt tctctttgtt tgtcaatatg attggatcga
     4861 tgccaggctc tttttttatt aaatcagacg cctgttctaa aatttctttt tctaagcgca
     4921 gtttgtatac ttcacgttga aggatatctc gttcctcttt caacaatttg atttcaggag
     4981 tgacattcat tgatttcaaa ggtttagtag ctccttttcc aagcagcttg tttttccata
     5041 agtacaatgc ttgtctggtt acgccaaaat catttgcgat tgatttagca gagagtgaac
     5101 gagtgcacag ggctaggaca gcagcttctt tttcttgttt tgtatagtgt atcgaatttt
     5161 gacgaacttt acgagtagat ggagcgtatt cttccaacca ttttcttaaa gtagttttat
     5221 ttggatagcc aaactttcga attgtacgtg cataacattg cccataatct aaataatgat
     5281 tcacagcaac ttctttttga gcaagcgtat atttccaaga agtacgttga tatttttgtg
     5341 cagcttatct gaagattgaa attctctaat ccattgtctg agggcaccaa cagaaggata
     5401 tccaagtttt ttagttgtag cagccaatgc ttgatcatat ttaaaatata aatcaacagc
     5461 ttttttccgt tcttcatatg tgtacatgag acgtcctcca aacttatttc agtccaagtt
     5521 tttgtccgca tgcccttgta tgtactttta ttctgccata aacagcttaa tgaatgtcca
     5581 cttgaaaaag tgaataaacg aaatagatga ctcgatttaa taaggaagta aacccacaca
     5641 gaaatatagg tgattttcct tgaatgacca agatattgaa agttagtgaa aaaacacaaa
     5701 aaggtagccc tattcacaga aggtctgcct tttgtgttat cgccagcaaa cattagaggg
     5761 gatatttcat atagcttttg tcctctcgac agtcctatca gtttctcttt taacttatct
     5821 ctaagtgctg tccagtttta atagcttcta gcttgtttcc taactgtgtt tttaatatgt
     5881 tgaaggcttt tgaacctgtg cagtggcaag taaggatttt ttctactcgt gagtcattca
     5941 atttatttcc aattttcatt acctcttgct caggtagacc taaattattt atataaggta
     6001 tgccaatcat atggaagcca ccgattaggt attttatggg tttagtcgga aatgtttcaa
     6061 gagcaatttc catcatatcg ataatgccat gatgactaca gccagtaaag acaatcagac
     6121 cgtcttcttg ctcaattacg accaataatt catctgaaaa atcatcataa acaaatttat
     6181 catccatctt tttgtataaa ttcttggtgg tcgagtttgc tttgcctagg gggatgatat
     6241 gaaagctcgg agcaatttct gttttcgttt ttattgtatg taaattctca tgcattttaa
     6301 ggatttcatt tttaggaata ccaacataca gaaacgtggt taaaatcttt aaataatggg
     6361 gtaaagttac accttcagct gtataaatag gggcgtttcc tgattttaaa tatcttaacc
     6421 cgccggtgtg gtcagaatga ccgtgtgaaa taatcactgc ctccagatca ttcaaagttt
     6481 cccccatttt agtcgcatta tagactaatt ttttattagc gccagcatcg aagagatatt
     6541 tcttccctga gtagttaatt aagagagata acccatatcc acttttaaag ttcaaattta
     6601 ttcttgagtt ttcaaataaa accttgattt ccactataac atctcctttc acatgaacgt
     6661 gtattcatat attgtagcac attaagttct attttttgtt gtaattaccg aaaaaaatat
     6721 taatggacgc tttttacgct ttatttcgta tgtggaagaa aatttcttag gatgtggcgg
     6781 gggattgggg caaagctaaa ttccaattac agtgatcatt taattagaca cctttaaata
     6841 cccacacttt catcaacact ataatttagg gaaagaaaag tgttggcatg atatagaaaa
     6901 acctttaatt aacagcgttt cctaaaattg gcacgcatct tgcttataga aaagcgtaag
     6961 gataggagga atgaaaatgg caactataat tcttgccagc catggggaat ttgctcaagg
     7021 attaaagcaa acagcgacaa tgattattgg agatagtgta ccaattcatg ccttgtcagc
     7081 atttagagat gaagatgaat ctattttggt acaaataaaa aaacttttag ccacaataga
     7141 tatagaagaa acctacattt taactgatat tctaggtggt agtgtgaata atgatatgct
     7201 cactattatt aaagagtatg agggattgca attaattact ggaatgaact tacctttagt
     7261 tatttctatt gcaacctata atggaaaaat tggggaaagt gagctagaaa agcttattag
     7321 agagagtcaa caatcagtga ttgattgtag aaaattatta gtagaacaac ttactggagg
     7381 agatgaccta tgattaaatt agtacgtatt gaccatcgtt tgttgcacgg acaggtagta
     7441 ttttcttgga gtaaatcatt aaatatcaat cgtattatta ttgccaataa agaggcagcg
     7501 aatgatgaat tcaaaaaaat gtctttgaat ttatctaaac cgactggcat taaattaaca
     7561 attttttcag ttgaacagtt actagaaaaa atgcctaaaa tcgaaacact taacgaaaat
     7621 atcatgctta tttttggtaa tacaaaagaa attcgtcaat tttgtgaagc ctatcctaaa
     7681 gtacaagaaa tcaactatgg agggataatc aaaaaagaag gttccaagca atttagtaat
     7741 gccattttct tgaatgaatc tgagcaagaa gatgccaaaa aattaaaagc tcttggcatt
     7801 aaacaattta ttcaacaggt accaacttct aaaaaagaag atttgaatac aatgttataa
     7861 aggaggaatg aaaatgctcg ttcagtcaat tggtttaggg ttaatcggtg ttttttgtat
     7921 tttagactca aggcttttag gaagaatgaa ttttgaacga ccgttgattg tcagtacgct
     7981 tgttggtttg gtacttggag acatggaaaa aggattaatt gttggagcct ctttggaatt
     8041 gatgtcctta ggattagtaa atatcggagc tgctgcacca cctgatatga atatggcatc
     8101 aattattgct acagcctttg ccattttatc aaatgcgaat gcagaagcag cattaacgat
     8161 tgccattcca atttcagtgt taggtcaaat actaggtatc ttaatgcgga cgattttgtc
     8221 aaatttgact cacgttgcgg atcgctttat cgaacaagga aaatttagcc aagcaaggag
     8281 aattcatata ctttatggct cgggattata tgctctcatg tattttattc ctatttttct
     8341 agcaatttat tttggaacga atcttgtttc tcagattgtt gatgcgattc ctgagtggct
     8401 aacaaatggc ttaacggttg caagtaaaat tttaccagca tatgggtttg cgttgttatt
     8461 acaaacgatg ttaacgaaaa aaatggtgcc atttttatta cttggatttt taattactgc
     8521 ttattctgga ttgggaatca ttggtattgc agccttcgca tgtattgttg cgtttgtcat
     8581 ggttcaagtt caaggtaaaa actcaaatgg taaccaaaat gagccagatc cattagatga
     8641 cttataagga ggtagattaa gtgaaagaga aagatcaaaa tgcatggaaa tatcggcagt
     8701 tcttatggcg ttcatgggca attcaagcct catggaacta tgaacgacaa atgaacatgg
     8761 gttttatgta cggaattgca ccaattctag ataacattta caagaatcca gaagatgaag
     8821 caatgaagaa agaagcttac gagcggcata tgatgtacta caactgtacc cctcaaacaa
     8881 gtgcatttgt gttagggtta actgcttcta tggaggagca atactacgaa gaccaagaaa
     8941 actttaatcc ggaatcaatc agtgcagtga agacttctct gatgggaccg ctatctggtg
     9001 ttggtgattc tttctttcaa ggaacgattc gggtattagc ctttggacta ggaattaatt
     9061 tagcccaaca aggtagtatt ttaggtccaa ttctagcaat gttaatttcc tttgtacctt
     9121 cctttttagt tacttactat ggtgggaaaa ttggctataa catggggaat aaatacttgt
     9181 ctaaactgta tcaagaaggt ttgatggata aagtaatgta tgtttgctcc attgtggggt
     9241 tgatggtcat tggttcgatg attgccagca tgatcgggat tacaacacca attcaaatgg
     9301 gtaaagcttt tgtcttacaa gatgtattag atagtattat gccacaattt ttaccattag
     9361 gattaacctt cttcatgtac tggttattgc agaaaaaggt caaaacagga tggatgttgg
     9421 caatctgttt agtcggagga attattttta gtttgttagg tatcttacaa taagggaata
     9481 accaataaat ttggttgaat aatataaata aggaggaatt caattgatga attcaaaaga
     9541 aattgttaaa gaaatcgtag cgaaaaaaga ggtaaaatca atcgtatttg ttggctgtgg
     9601 cgcttcaaaa gcggaccttt atcctgcaaa gtattttcta gaccaaaatg cgaagcagtt
     9661 acggattagt cattacacag ccagcgaatt taactatgca acaccagcat ctgtagatga
     9721 gtcaactgtc gttatttcag cttctttagg aggtacgact ccagaaacag ttgaagctaa
     9781 cgcagtagct aaagaaaaag gtgctacagt tattagtcta acacgaagca ttgactcacc
     9841 gttaacaaaa gatgcagatt atgtaattta tcatcgattt gcagagaact atgctgctaa
     9901 attagaaaaa acaggctact gtttagaatt ggcagtagaa cttttacatc aagtagaagg
     9961 ttatgaccat tatgaagcaa tgcaagaagg atttgctact atctatgatc ttgcccaaaa
    10021 aagtgcagaa tcagctcgta aagatgcgaa gaaatttgct gaacgcttta aagatgaaaa
    10081 agttatttat gtaatgagta gtggtgctac acatgaagta gcatactcaa catcaatttg
    10141 cttgatgatg gaaatgcaat ggattaattc tggtaccttc cattcaggag aatttttcca
    10201 cggaccattc gaaattgtgg ataaagacgt accgtttata ttattaatga atgatggaaa
    10261 aactagaaat atagatgctc gagcattaac tttccttgaa cgctttgata cgaaaacaga
    10321 agttgtagat gccttggatt atggcttgtc ttcacatatt gctaaagaag tcttagacta
    10381 ctttaaccca ttcgtgatta ctgcagtatt tagagtttat gcagaagaat tgtcagaagc
    10441 aagagaacat ccattaacta agagacgtta tatgtggaaa ctaacttatt aaaaaataga
    10501 tcaaactaaa taaggtggct tgagaaatta agccaccttg ttttcttcaa tgtgatttta
    10561 tacatatggt aaaataacta gtacatatgg taaaataact agtacatatt gtgaaagcag
    10621 gtggcaagat gcagcgatta aatgactatc tttatcaact aacattgctt ttgaaatcta
    10681 cgccttcaga taattattca gctcgcagta tttcgcaagc tttacagttg aatagaagta
    10741 caatcagtag ctacttaaat gaaggtgttc gtgaagggct tttcataaaa gtaaaaagtt
    10801 acccggtttt atttttacat agaacagctc ttgaagaact acatatcaca ctgaataact
    10861 ccgaaataga atcaattgaa agcttgttaa caatttctca aaaacccgca ttagatcaag
    10921 taattggctc taaaggaagt ttgaaagaag caattgatca aattaaaacg gcagtcttat
    10981 atcctggaaa agggttgcct ttattattga tcggcgctag tggatcaggg aaaacctttc
    11041 tagcgaataa aatatatgaa tatgcagtag aagaaaaggt cattcacaca tcggcacctt
    11101 tcatagatta taattgtgca caatatgtga gcaatccaga gttactatcc tctgctttat
    11161 ttggctacac gaaaggagca tttaccggag caagccagga gcatactggt ttgctagaaa
    11221 aagccgatgg aggagtattg tttttagatg aagtgcatcg cttatctgaa gaagggcaag
    11281 agaaattatt tacatttatg gatacgggcg aattctctcc tatgggggat aatagtattc
    11341 ggaaaaaagc cgatgttcga ttagtctttg caacaacaga aaatatatat acgactttct
    11401 taccaacttt tcttaggaga ttgccagtta ttgttaatct acctagattt caacaacgtc
    11461 cgtcgtttga acgtctgtca ttgattgatg aattttttgt ttctgagagt aaaattttag
    11521 ctaaggaact atctgtttct gatgcattga ttcatttctt aatgaatagt gattttgaag
    11581 gtaacgtggg gaatattaaa aatattatca agtatgcttg cggaaacgca tatatccacc
    11641 agaagagtca gcaaacaatt caagttaaat tattggatat gcctttagag tacagttata
    11701 aattcaagga gcaactaaat aaacccagaa agaaaagagc agatcgaatc tactatccag
    11761 aagaaacacg acaagtgcaa ctagaaagaa acaatcagct ttttgttcaa ttttttgacc
    11821 aattacttac tggattctct gaagtgattg aaaataacaa ggatattaaa ctatttttag
    11881 aagaaatggt taccaaggta acacaagtaa tggatacgct tatttttcaa gaggattacg
    11941 aaaaagagca gtctttatac gccattctaa actatcatat tcgtcaaagt attgatttta
    12001 tgcgagaaag ctacgggttt gaacaagatg gtaaccgggt agtcgctttg gccaactatc
    12061 tatatgtcaa ggataatcaa gtgatttttg aaaatagtct tcaagaa
//
LOCUS       DAZKAS010000081        12105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000081 DAZKAS010000000
VERSION     DAZKAS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.083"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1615)
                     /locus_tag="ACG8H7_001846"
     CDS             complement(56..1615)
                     /locus_tag="ACG8H7_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFP8983926.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1770..4004
                     /gene="pcrA"
                     /locus_tag="ACG8H7_001847"
     CDS             1770..4004
                     /gene="pcrA"
                     /locus_tag="ACG8H7_001847"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFP8983927.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4021..6057
                     /gene="ligA"
                     /locus_tag="ACG8H7_001848"
     CDS             4021..6057
                     /gene="ligA"
                     /locus_tag="ACG8H7_001848"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP8983928.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6163..6468
                     /gene="gatC"
                     /locus_tag="ACG8H7_001849"
     CDS             6163..6468
                     /gene="gatC"
                     /locus_tag="ACG8H7_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFP8983929.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6468..7940
                     /gene="gatA"
                     /locus_tag="ACG8H7_001850"
     CDS             6468..7940
                     /gene="gatA"
                     /locus_tag="ACG8H7_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFP8983930.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7937..9367
                     /gene="gatB"
                     /locus_tag="ACG8H7_001851"
     CDS             7937..9367
                     /gene="gatB"
                     /locus_tag="ACG8H7_001851"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFP8983931.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9377..10432
                     /locus_tag="ACG8H7_001852"
     CDS             9377..10432
                     /locus_tag="ACG8H7_001852"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP8983932.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10543..11916
                     /gene="rlmD"
                     /locus_tag="ACG8H7_001853"
     CDS             10543..11916
                     /gene="rlmD"
                     /locus_tag="ACG8H7_001853"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP8983933.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11919..12047
                     /locus_tag="ACG8H7_001854"
     CDS             11919..12047
                     /locus_tag="ACG8H7_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP8983934.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         4189 a         2232 c         2629 g         3055 t
ORIGIN      
        1 atagaatcaa gtagtgtaga tgaatagttg ttgtaaaata gtttttccat gttcgttaaa
       61 atgattgctt caaatttgtg atagaagatt tagcctcgat ttcctgcttg attcttggca
      121 agtttgcttc atcagtcaag ataaccaaaa tatctcctac attcatgatc gtatcaccgt
      181 gagtcaagat ttcctgattt cctctgcgta tagagattag cagcatattt ttaggccaat
      241 tgaagtctcg taccattaat ccgtctaaag aactctctgc cgttactgga aaatcaaaag
      301 ttgtccttgc accttttagt gaggcggtct gagtatttcc aagtagacgt tccaataatg
      361 attcgtaaat tggattaccg ccgagaaggt cattgacgat ataagcaaca agagcagcca
      421 atcctaaagg catcagatga ttaagagagc caaccatttc tgtcactaaa ataattgctg
      481 tcagcggtgc ttttccaatc gctgtgaaat aaccagccat ggcaataacg acaaaatcct
      541 tgatgtagat tggatccatc ccgaaatcaa ccataatgtt tccataaaag ctaccgatta
      601 aagcccccaa tgtcaatatc ggaaggaaaa tcccccctgg aagaccccca ccgtaagaaa
      661 tcatggaaaa aacgaatcga agaacaaaca aaccgataat cataatgatt ggcaaggaag
      721 catgtccaaa agctaaaacg atttgattgc ctccgcctag agtactaggc cataaataac
      781 caattggcaa aatcagcaag aaaggaacga acccgtgaaa ataagctggt aaaggcaatc
      841 ggttgtaaaa ttttggcaaa gccagcaaga cattctgata aataaaacct aagatgccta
      901 gaagtactcc taagatcact aaggaaccat aatatttcaa cggcaaagaa ggaaggtcac
      961 ctagatataa aacaggtctc aagccgaaaa agtaaagaga gacgaagtta gcagtgattg
     1021 ctgaagcaaa tgatgtcagc caaacaagtg gagaaaagga gtgatggatt tcttctaaaa
     1081 caaagagtaa tcctgcgata ggagcattga atgctgctgc aagtccgcta cttgcaccgc
     1141 tagaaattaa aatcttttct tctgaagatg gactccgaaa caattgtcca gctccttgtc
     1201 caatggatgc gccaagctgt atagaaggtc cttctctacc tagaaaaagg cccgcaccaa
     1261 ctgaaagtac accgccaatg aactttttcc ataaaacaga gaaccagttc attgataatt
     1321 ccccgcgaag ctgtccctct acttgtggaa tcccgcttcc cttgatatcc ggttcatttt
     1381 tgaccagata acccacgaaa aatgctgcaa ggatgcagca aatagctagg ccaattaagt
     1441 aaatcggctg ttcccgactc atttcataga tacttcgaac taaatcgctt agcttttcga
     1501 tagataatcg aaacaggcta acgacgattc ctgtcagcag tccaacaaat attcctttta
     1561 gaataaaata tatctgagtc ttatctaatt tcttgatttt tccttttttt tccattcaaa
     1621 cgcctcgctt tcttttaatt tagcgcgaaa tcaacgaatt gacaaggaaa aatccataaa
     1681 aagaaacaaa tgattttgaa ctagctagtt taatgaaagc caagaaaatg ctataattaa
     1741 caagattgat aaaagaaagg atgttgttaa tgtcatccaa agctgaatta ttaaacggga
     1801 tgaacccccg tcagaaagaa gctgttcttc acactgacgg accattactg ctaatggcag
     1861 gtgcaggaag tggaaagacg agagtgctga cacaccgcat tgcctatttg attgaggaaa
     1921 aagaagtcaa tccttggaac atcctagcga ttacttttac caataaggct gcaaaagaaa
     1981 tgaaagaacg tgtcaatgcg attctcgcat ctggaggcga agatgtctgg gtatcgactt
     2041 ttcactcgat gtgtgtgcgt attttaagaa gagatgttga ttttatcggg tataaccgga
     2101 actttacaat catcgattct tctgaacagc tgaccttgat gaaacggata ctaaaagaac
     2161 tgaacattga tccgaaaaaa tatgatcctc gaagtatatt aggaacaatc tctcaagcaa
     2221 aaaacagcct gcagacccct caggattttg caaaaatgca aggcagttat tacgaagaga
     2281 ttgcggcaaa atgttatgct gcttaccaaa aagagcttca atataatcaa tgtatggact
     2341 ttgacgacct gatcatgaat acgattcgat tatttgaaga acatcctgat tcactcacgt
     2401 attatcaaaa caaattccat tatattcatg tagatgagta ccaagataca aatcacgcac
     2461 agtatacgct agtcaattta ttggcaggta gatttaggaa cctgtgcgtc gtgggagatg
     2521 cagatcaaag tatttacggc tggcgcggtg cagatatgca gaacatcttg gattttgaaa
     2581 aagattatcc agatgctgca gtaattttgc tagaacaaaa ctatcgctcc acgaaaaaca
     2641 tccttagcgc agcaaaccaa gtcattgaaa acaatagcaa tcgaaaacca aaaaacttgt
     2701 ggacagaaaa caaagaagga aacaagatca cttactaccg tgcagataat gaacgagacg
     2761 aaacccgttt tatcgtagac cggatgcaag aagagattcg aagcaatcat cggaattacg
     2821 gcgactttgc catcttgtac cggacgaacg cacaatctcg tgtaatggaa gaaactctgt
     2881 tgaaagcaaa tattccttat aaaatggttg gagggcacaa gttctacgat cgtaaagaaa
     2941 tcaaagatat tttagcgtat ttgaatgtgc tggtaaatcc tcaagattca atcagttttg
     3001 agcgtatcgt taattcgccc aaacgaggca tcggaccagg atctatcgaa aaactacgca
     3061 gttttgcttc tcttcatgaa tggccactgc ttgaagcagc gcaaaatgtg gatctggcaa
     3121 atatcggtgg aaaagcagga cagcaattag gtgcatttgg agagatgatc caagaagtca
     3181 ctcagatgat cccatacttg acagtgacag aattgacgaa agaggttttg gatcgtagcg
     3241 gatatctaga agacttaaaa atccaaaata cattagaagc acaagcacgc atcgaaaact
     3301 tggaggaatt tttaacggtc acacaagaat tcgacaaaca attcgaacag caaaatgaag
     3361 aagacgctga tgcaccagaa gaaaaactaa cggtcttctt aaatgatctg gcacttgttt
     3421 ccgacattga caatttagaa gaagatgctt cccaagtgac attgatgact ttgcatgcag
     3481 ccaaaggatt agaatttcca gttgtattcc tgattggtct agaagagggg gtttttcctt
     3541 tatctcgagc attgatggaa gaaagcgaac tggaggaaga acgccgtctc gcatatgtgg
     3601 ggatcacgcg agcagaagaa gcgttgtatt taacaaatgc tttctccaga acattatatg
     3661 gacggacgca atataaccga ccttctcgtt ttgtagaaga aatcgatcag gagttactgg
     3721 aaatcgaagg catgcgtcct acgcctaaaa aaacgcctgt attcgctaaa aaaacagcat
     3781 atagctataa acagccagag acagcggttg ttccctctaa aagtgctact ggaggggaaa
     3841 ataacagctg gaaacctgga gataaagtca aacacaaaaa atggggtcta ggaacagtcg
     3901 tccgagtaag cgggacgtcg aaggatctag aactagatgt ggctttccca agtcaaggag
     3961 taaaacgtct tttggctgcc tttgcgccaa ttgaaaaagc ttagtagtgg agggtaaatg
     4021 atggaacatg aaatgacgat tgacgaagct gctaaacgag cagaagaact tcgtacaaga
     4081 ctgaaccaat ggtctcgcga atactacgta gaagataaac caacagtgga agactatgtt
     4141 tatgataaag aatacgcaga attagtagca atcgaggaac aatatcctga tttgatcacg
     4201 tctgattctc ccactcaacg agtaggcgga aaagtattag aaggttttga aaaagtcact
     4261 catgatattc cgctttacag tttgaacgac gttttcagca aagaagagct gatagctttt
     4321 gatcaacgtg tacaaaaagc agtgggacgt gtagttgact attgctgtga actaaaaata
     4381 gatggacttt ccgtttcttt gcgttatgaa gatggaaatt tcgtgagagg tgcgactcgc
     4441 ggggatggaa ctgtaggaga aaacatcact gaaaacctaa aaactgtccg gtcggtccca
     4501 atcaaattaa aagaaccaat gaacatcgaa gtaagaggcg aatgtttcat gccaaaacgg
     4561 tcatttgtcc aattgaacca agacagagaa gcagagggaa aagatatctt tgcgaatcct
     4621 cggaacgccg cagctggttc tttgcgtcag ttagattcta aaatcacagc gaaaagaaat
     4681 ctggacactt ttttatacac agtagctgat ttcggtccta tgcaagcaaa aacccagtat
     4741 gatgcgctag aagagctaga aaaaattgga ttccacacga atcgggaaaa acgtctatgt
     4801 cattcgattg atgaagtgtg gtcatacatt gaagaatatc atgacaaacg tgtggatttg
     4861 ccctatgaaa tcgatggaat cgtcatcaaa gtgaatgaat tctcgctaca agatcaactt
     4921 ggttttacta tcaaagcacc gagatgggca gcagcttaca agtttccgcc agaagaagtt
     4981 gaaacgctaa tcgaaaacat cgaatggaca gtcggtcgaa caggggtagt aacgccaaca
     5041 gctatcatga caccagtccg agtagcagga acgacagtaa gccgtgccag cctgcataac
     5101 ggtgattata ttaagttgaa ggatatccgc ttgaaagata cagttctgat ttataaagcg
     5161 ggagacatca tcccagaagt ttcgcaagtc gtgttagata aacgacctaa agacagcgaa
     5221 gaataccaat tgcctacaca ttgtccagtt tgtggaagtg aacttgtcca tttggatgaa
     5281 gaagtagcct tacggtgcat caatcctaaa tgccctgctc aaatgaaaga aggtttgaac
     5341 cactttgttt caagaaatgc gatgaatatc gatgggttgg ggccgcgcgt actagaacag
     5401 atgtatgata aaaaactagt agcagatgtt gctgatcttt acaaattgac agaagaagag
     5461 ttattgacgt tggacaaaat aaaagaaaaa tcagcaaata atattttgac agcaatcgac
     5521 aacagcaaag ataattcggt cgagcggttg attttcgggt taggaatccg acatgtcgga
     5581 gcgaaagcag caaaaatttt agcagaacat ttcggtgatc tagaaacatt gagtaaaagt
     5641 gactatgagt cgattattgc gcttgatacg atcggtgata tcattgccga cagtgtagtc
     5701 acttatttca gtaacgaaga agtccatgaa ttgatgaatg aattaaaaca agcaggcgtc
     5761 aatttcgaat acaaagggct tcgaaatgcc caattgcaag aagtcgagtc tccttttaaa
     5821 gaaaaaacag tagtccttac tggtaaacta acacgattta ctcgtgaaga agcaaaagag
     5881 acgatcgaaa atcttggcgg gaaagtgact ggtagcgtat cgaaaaaaac ggatatcgtg
     5941 gtagcaggag aagatgctgg cagcaagctg accaaagcac aagaattagg aatcgaagtt
     6001 tggacagaag atcaaatggc agatgcattg gctaaaagtc gctcagtaga agaatgaaaa
     6061 atgagttttt tcagacaaat ttcaataaac aatttcagac acggcactct tgtgttaaaa
     6121 tagagagcaa agtttttaac tcgtttagaa agaaggggta gtatggcgat tagtgaagaa
     6181 caagtgaaac acgttgccaa attagcaaaa cttgcgttcg cagatgaaga acttgcaagt
     6241 tttaccgacc aattaggtaa aatcatcgat atggttgaaa tgctggaaga agtagatacg
     6301 gaaggtgttc catttacgtc gaatgtggtt gaaacagtaa acgtgatgcg acaagatcgg
     6361 gcgcaagctg gatggaaccg cgatgaatta ttgaaaaatg tgccggaaac agaagacggc
     6421 tttatcaaag tacccgcaat catcgataat ggggaggctg gcgcataatg acagaattac
     6481 acaatcgttc tctagaagaa ctgcatcata tgcttgtatc gaaagaaatc actgtacaag
     6541 atttgacaaa aacaacattt gaacgaatca aagaaacaga gcctgaggtc gaggcattca
     6601 ttacattgaa cgaagaaaaa gcattggaac aggcaaaagc tttagatgaa aaagggattg
     6661 atgaaagcaa cgtccttgct ggtattccaa ttggtatcaa ggataatatc gtaacaaaag
     6721 atatcttgac aaccgctgca tctaagatcc tttacaattt tgaaccaatt tatgatgcaa
     6781 cggtcatgga taaaatctat caagcagata tgatccctgt tggtaagcta aacatggatg
     6841 aatttgctat gggtggaagt acagaaactt cttatttcaa gaaaacaaaa aatgcttggg
     6901 acccaacaaa agtacctggt ggctcttctg gtggttctgc agcagcagtt gcagctggac
     6961 aaattccagt ttctttaggt agtgatactg ggggaagtat ccgccaacca gcagctttta
     7021 acggcatcgt agggatgaaa cctacttatg gccgtgtctc acgttttggt ttgattgcct
     7081 ttgcttcatc tcttgaccaa atcggaccaa tgacccgtac ggtgaaagat aatgcattag
     7141 cattgacagc tatcagcggt tatgacgaaa aagacggtac ttcttcaggc gtttcagttc
     7201 ctgattttgc agagggactg acaggcgaca tcaaaggaat gaagattgct ttacctaaag
     7261 aatacttagg cgaaggtgtg gcccctggcg taaaagaagc tgtattgaaa gcagcagaaa
     7321 cattccgttc attaggtgct gttgtggaag aagtcagctt gcctcattca aaatacggtg
     7381 tggctgttta ttatatcatt gcctcatcag aagcaagttc caatttgcaa cgattcgatg
     7441 gtatccgcta cggttatcgc tctgaaaatg tgaaatcatt agatgatgta tatgtcaact
     7501 cacgttcaga aggattcggt gaagaagtaa aacgccgtat catgttaggt acattctcct
     7561 taagcgcggg ttattatgac gcttacttca aaaaagcagg acaagtccgt acattgatca
     7621 aacgagattt tgaaaaagtc tttgcagatt atgacttgat catcgggcca tcttcaccaa
     7681 cagttgcatt cggaatcggt gagaacatta acgatccaat cacaatgtac atgagtgaca
     7741 tcttaacgat ccctgtcaac ttggctggac ttccaggtat gtctgtaccg gcaggtttat
     7801 cagaaggatt accagttgga ttgcagatca tcggtaatta ttttgatgaa aaaacgatgt
     7861 atcaagcggc atatgcgttt gaacaagcaa cagaattcca taaacaacaa ccagctattt
     7921 taggagggaa agacgaatga attttgaaac agtcattgga ctagaagtcc acgtcgagtt
     7981 aaaaaccaac tcaaaaattt tctctccagc gccagctcat tttggtgcag aaccaaacag
     8041 caatacaaac gtgatcgact ggggttatcc aggcgtgctg ccagtaatga ataaagctgc
     8101 gctggaattc gggatgaaag cagctttggc attaaactgt gaaatctcta aagatactca
     8161 ttttgaccgg aaaaattact tttacccaga taatccaaaa gcatatcaaa tttcccaatt
     8221 tgatcaacca atcggtcatg acggatggat cgaaatcgaa gtagaaggta aaaagaaaaa
     8281 aatccggatc gaacgtgtcc atttagaaga agatgctgga aaaaacatgc atggtattgg
     8341 cggttattct tacgttgatt tgaaccgaca aggcacgcct ttgatcgaaa tcgtttctga
     8401 agctgatatg cgttcaccag aagaagcata cgcttatcta gaagcattgc gatcaattat
     8461 ccaatttaca gaagtcagcg atgtgaaaat ggaagaaggc tctatgcgct gtgatgccaa
     8521 tatttctctt cgcccttatg gacaagaaga gttcggtacg aaagcagaat tgaaaaactt
     8581 gaactcttta tctttcgtga aaaaaggttt agcttttgaa gaaaaacgtc aagcaaaagt
     8641 tcttctttca ggaggagaaa tccaacaaga aacacgccgt tttgacgaaa caacgaataa
     8701 aacagttttg atgcgtgtca aagaagggtc tagtgattat cgttacttcc cagagcctga
     8761 tatcccgaaa ttcgtgatcg atgacgaatg gattcaaaaa gtaaaagcta gtttgcctga
     8821 gatgccagct tctcgtcgcg aacgctatat ccgtgagttt ggacttccag agtatgacgc
     8881 aatggtattg acacttacaa aagaaatgtc tgattttttt gaagctgttt tggaaaatgg
     8941 tgcagatgcc aagcaagcat ctaactggtt gatgggtgaa gtttctgctt acttgaacag
     9001 tgagaaactg gaattagctg agacaaaatt gacacctgag aacttagcag gaatgattcg
     9061 tttgatcgcc gatggaacga tcagctcaaa aatcgccaaa aaagtcttcc gtgaattgat
     9121 cacaaatggc ggagaagccc aagaagtagt tgaaaaaaat ggttgggtgc aattatctga
     9181 tccgaacaaa ctactgccaa tcattaatga aattttggat aacaaccaac aatctgtcga
     9241 tgatttcaaa aatggaaaag atcgtgcggt tggcttccta gtcggtcaaa tcatgaaagc
     9301 aactaaagga caagcaaatc ctggtgtcgt gaataagcta cttaaagatg aattagcgaa
     9361 acgatagagg ctgaaaatga aaaaagcacg cgtaatttat aaccccacct cgggaaaaga
     9421 attattgaaa aaaaatttgg cggatatcct acaggcatta gaagaagcag gatttgaagc
     9481 cagcgcctat gcaactacac cagaaccaga ttcagccaag aatgaagcat gtagagtcgc
     9541 agaactaggt tttgatctag tagtggcagc tggtggcgat ggcacaatca acgaagtggt
     9601 taatggaatc gctccgcttg aacaccgtcc taaaatggcc atcatcccag ctggtacaac
     9661 gaacgactat gctcgagcat taaaaattcc tagagataat attaaagcag cagcagaagt
     9721 gataaaaaaa caccaaaccg taaaaatgga catcggcctt tctgataaca gttacttcat
     9781 caatatcgct gcaggtggct atttgacaga attgacttat gaagttccat cagaactaaa
     9841 gagtattttt ggttatcttg cttatcttgc gaaaggagca gagatgctcc ctcgagtaaa
     9901 gccaatcaaa atgcgcttga agtatgatga aggggaatat atcggaaatg cttcgatgtt
     9961 tttcctaggg ctgacgaact ctgttggagg attcgaacag atcgcgccag atgccaaatt
    10021 ggatgatggg aagttttctt taatcattgt gaagacagca aatatctttg agatcttgca
    10081 tattgcagca ctcatgctga atggcggtcg acatgtcgat gatccaagaa taatctatac
    10141 caaaacaagc agtttgttcg ttgaaacaga agatgatatc aatcgaccag taatgatcaa
    10201 cttagatggt gaatacggcg gggaagcacc gatgcatttc caaaatcttc atcaacacat
    10261 tgagttcttt gccaataccg atcagattcc tgatgaagca atcacaggta cagatgaaga
    10321 agaattagag gttgtcagca aagaattcgt aaaagaagtc gaacgtttaa cggatgaaga
    10381 tatcgacggt gacggaaaaa tagcagacga aacacctaat caaaagaatt aatcaaaagc
    10441 cggtgctgaa cgtagtaagg tcagtgccgg ttttagttgt gtaattagct gatttatagt
    10501 agagttcagt aggattgaaa aataggctta aaggagcaaa atatgacaga acagcaaata
    10561 gtaaagaaaa acgaacgata tacagtcgaa atcgtcgact tgagttatga aggaatgggg
    10621 gtagcaaaaa tcgatggtta tccattattt atcgaaaatg cactccctgg agaagaagta
    10681 gaaatactag tagtaaaagc aggcaataag tttggatatg gaaaagtcga aacatttatt
    10741 acacaatcac ctgatcgtca gcctgtagaa aacaatgcat tacttcgcat aggtatcgca
    10801 cctttagccc atttgaagta tgaacaacag cttgtcttca aacaaaaaca agtagagcat
    10861 gtaatggcga agattgctaa gatgccagaa gttcctgtac ttccaacggt cggaatggca
    10921 gaaccattcg gctaccgtaa taaagcacag atccctgttc gccgtatcga tggacagtta
    10981 gccaccggtt tttacaaaaa gaatagccat gatctagtcc ctatcgaaga tttttatatc
    11041 caagatcctg tcatcgatca agcaatcaaa gtagtcagag atatcttgca acggttccaa
    11101 gtacgaggat ataatgaaga aaaaaatgaa ggacagcttc gtcacatcat tatccgtcgt
    11161 ggtcattatt cccatgaaat gatggtcgtt ttagttactc gtaaagaaaa attctttaaa
    11221 tccaaggaaa ttgctgaagc tattcatgaa gaattgccag aagtggtttc ggtcatccaa
    11281 aatatcaatg aagaaaaaac aaatgtgatt ttaggagaca aagaaaaagt tttatatggc
    11341 agagactata tcgaagatca gctattagga aaaacctatc gaatctcagc aaagtcattt
    11401 tatcaagtaa atactcaaca agcagagtat ctgtaccaaa cagccatcga gtttgccgag
    11461 ttagataaaa aagacattgt gatcgacgct tattcaggaa taggtacgat tggcttgtct
    11521 ttagcagaca gagtggataa ggtgtatggg atggaagtta ttccagaagc aattgaagat
    11581 gcacagttca atgctttaaa taataaaatc gaaaatgttc attatgaagt cggaaaagca
    11641 gaaaaagtta tgaaaaaatg gcaagaaaaa gggatcaaac catctgtgat cgtagttgac
    11701 cctccacgaa aaggattaga tcatcatttt attgaatcag caatcgaaat gtcaccagaa
    11761 cgaatcgtct atatctcatg taatccggca acttttgcaa gagatgctaa gttatttgca
    11821 gaagcaggtt atgccgtgaa gaaagttcag cctgtagatc ttttcccgca aacccatcat
    11881 atcgaattag tcgcttcatt cgttaaagga gaataaaaat ggaccaaaaa gaaaaaatga
    11941 aagaattgat caaaaagaaa caaggtggag gccgatccaa acaaatggaa acacctaaga
    12001 atgatcgtaa aaacatgaga aaaggcccta aaatctttaa taaatagacg tgctaaagaa
    12061 agactaagaa gtgttaactt ttgttattgt ttcttagtct tttta
//
LOCUS       DAZKAS010000082        11990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000082 DAZKAS010000000
VERSION     DAZKAS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.084"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="ACG8H7_001855"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="ACG8H7_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFP8983935.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="ACG8H7_001856"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="ACG8H7_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP8983936.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="ACG8H7_001857"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="ACG8H7_001857"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP8983937.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="ACG8H7_001858"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="ACG8H7_001858"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFP8983938.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /gene="rpsF"
                     /locus_tag="ACG8H7_001859"
     CDS             6088..6387
                     /gene="rpsF"
                     /locus_tag="ACG8H7_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP8983939.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="ACG8H7_001860"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="ACG8H7_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP8983940.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /gene="rpsR"
                     /locus_tag="ACG8H7_001861"
     CDS             6975..7211
                     /gene="rpsR"
                     /locus_tag="ACG8H7_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP8983941.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="ACG8H7_001862"
     CDS             7354..9327
                     /locus_tag="ACG8H7_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFP8983942.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="ACG8H7_001863"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="ACG8H7_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP8983943.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="ACG8H7_001864"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="ACG8H7_001864"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP8983944.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4005 a         2178 c         2655 g         3152 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag accaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
     4441 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
     4501 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
     5881 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa
//
LOCUS       DAZKAS010000083        11793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000083 DAZKAS010000000
VERSION     DAZKAS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.085"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..341)
                     /locus_tag="ACG8H7_001865"
     CDS             complement(162..341)
                     /locus_tag="ACG8H7_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983945.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(357..557)
                     /locus_tag="ACG8H7_001866"
     CDS             complement(357..557)
                     /locus_tag="ACG8H7_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983946.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(722..1429)
                     /locus_tag="ACG8H7_001867"
     CDS             complement(722..1429)
                     /locus_tag="ACG8H7_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983947.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1422..1859)
                     /locus_tag="ACG8H7_001868"
     CDS             complement(1422..1859)
                     /locus_tag="ACG8H7_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983948.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1874..2125)
                     /locus_tag="ACG8H7_001869"
     CDS             complement(1874..2125)
                     /locus_tag="ACG8H7_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983949.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2125..2331)
                     /locus_tag="ACG8H7_001870"
     CDS             complement(2125..2331)
                     /locus_tag="ACG8H7_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFP8983950.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2768..3028)
                     /locus_tag="ACG8H7_001871"
     CDS             complement(2768..3028)
                     /locus_tag="ACG8H7_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983951.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3202..3306
                     /locus_tag="ACG8H7_001872"
     CDS             3202..3306
                     /locus_tag="ACG8H7_001872"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFP8983952.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="ACG8H7_001873"
     CDS             complement(3447..3932)
                     /gene="ssb"
                     /locus_tag="ACG8H7_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP8983953.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3935..4834)
                     /locus_tag="ACG8H7_001874"
     CDS             complement(3935..4834)
                     /locus_tag="ACG8H7_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP8983954.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4847..5440)
                     /locus_tag="ACG8H7_001875"
     CDS             complement(4847..5440)
                     /locus_tag="ACG8H7_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFP8983955.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5516..5665)
                     /locus_tag="ACG8H7_001876"
     CDS             complement(5516..5665)
                     /locus_tag="ACG8H7_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983956.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5681..7885)
                     /locus_tag="ACG8H7_001877"
     CDS             complement(5681..7885)
                     /locus_tag="ACG8H7_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFP8983957.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7896..9356)
                     /locus_tag="ACG8H7_001878"
     CDS             complement(7896..9356)
                     /locus_tag="ACG8H7_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP8983958.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9417..11789)
                     /locus_tag="ACG8H7_001879"
     CDS             complement(9417..11789)
                     /locus_tag="ACG8H7_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP8983959.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2931 a         2465 c         1981 g         4416 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaagtgcta tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt
      181 acctcaaatc ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg
      241 gattcacttt tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc
      301 aacgtcacgg gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat
      361 cgaaataacc ctattaaaaa tatggctgca atcaacttga atattagact cacaagggtt
      421 aacacaaagc gccaaaagat caaacaaaat accagtattc ctaagaatac tacgaagttt
      481 aaatgtagag ctgcaccatg acgcataagt atctcgccaa caaacagtac gaacaataga
      541 ggaatgattt tattcatatg gatctccttc tttctcttag gttatcgggc tttgcccgct
      601 tatttcgcac cacctaagcg gcgggggcgg ttggatcgat tggtgcgaaa taagaaccat
      661 tcccttaatt tcaaggtttt ttgttcttat ttcacacatc ctcacttttc ggtataaatg
      721 gttattgacc ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat
      781 tttggatttc ttctgtatcg tcgtaagtgt aggtgtctaa aaaatcagtt aaagtcgtgt
      841 accagtcgtc gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta
      901 cagtgagttc ataatccatt cccattaaca cgatagattc cgtcccacac gtcaaaaatc
      961 tagtaaatac aaggtaataa tcgtctaccg tcgcatcttt gctgtttagc tcttccattt
     1021 cttcttccat taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt
     1081 gtttcctgca aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa
     1141 cattcatgat atatttttct gtttctagtc gtagaagctc cgctcctttt tttaatgtgt
     1201 caggacgacg tttatacacg tctaaacgat tatggctgac tgtaaccttc gtttgactcg
     1261 tttcactttc catatacaac tgttcttcca ctctttgaat aaagtacttc atttcttcca
     1321 tacaactaaa gtctttttga atccgtctga ttacttcatt caagtgttct ttgtttgtta
     1381 aaaattgcga aacatcttct gttgttagct gataaaccac cttattcatt atctttctcc
     1441 tccgattcaa tcgtataaaa tgcaccattt ttttcgatat tttcaaaagt atctagccag
     1501 tcgctagcca ttgctttagc tttcttaatc tgtgcgaatt ttgcataagt cagttctaag
     1561 gcttgatatt cggaaggttt gatagtgctt ggtttgacac ctacttccaa gcatagtaag
     1621 gcttggaaaa accattttcc attttgtttt ttcagttctt ccagtgttcg atagtacgtc
     1681 gcaatgagat tcatttcgac acctgattca atcatttctt ccactgcgac tgacaatttt
     1741 tccttgtaat gttttttggg tacacgtgtt tcatggtata ggaacgtcat atcctccatt
     1801 ttttgtgcca ttttatccaa aggtaatttg acaaattcgt catagtaatt ttgagccata
     1861 ttttttctcc tttttagttc tctaatttgg ttaaccaata ttcgatttca taagtttcca
     1921 tgttttcttg ataatgcagt tcatatcctg caatcttcac taggtcttca aatagcatag
     1981 tcggtgcatc ctctactaaa ggggtttcta tcgtccagta ccgcggaata agtaaagctt
     2041 ctgctacttt ttcagaaatg acttctttgt ccaccaaaat ttgaacgact tcttttacag
     2101 tcaataattt gtgttgtgtt ttcatcaccg tttctttact ttctcatcaa tcaaaacata
     2161 atcgccataa gacaaggtat attttttccc gcatgttgga cattgataca aacgatagct
     2221 tttgatacat ttcgctcttt ctccacaatc gcattcgaca gtatgtggtg catgatagtt
     2281 tgggaataat tcgctgaagg gaacacccca ataatcaaat ttctttcgca tgattgattc
     2341 tgcctcctct taggaaaggt tttatttcct ttcgttagtt ctttctttat tgatttgttg
     2401 gccagaatga atcaacgaaa agcgacaagg acaagggcgc gcagcgtgcc atttaccctt
     2461 gtccttgtcg ctttttggtg ataaaaggca acttgttgcc ggtctggcaa atcaataaag
     2521 agatagcgaa caggaacgaa ccttttggtg agaggaagtt ttttctattt tgtcttgctt
     2581 tgcaagacac catatcttag tgaacgtgca acgttcactt cctttcttct cgaacggcgg
     2641 tggcggttgg agtgagagaa aggtttttca attgagaagg cacttgtgtc ttcctaaaaa
     2701 ggtcttttcc tgtttctatg taaaacaaaa agagctgcaa tcctcattta ggactgcagc
     2761 tcttctttta tatgcccatc gatcgcgtaa aatgatcggg gtttctaatt tgcatcgatt
     2821 caatgactaa ttgattccgt ttattgaaat gacctgtgac ggcaatattg tattttccat
     2881 catccataat taacaattta tcgataattt caatatctgc aactacacaa ttaatcgatt
     2941 catgaattgt ttgtaaagta aagcgaacca ataatggttg tgtttttatt actcggattt
     3001 tatcaagcaa accaacatat ttgttcatca cgatcacctc gctaaaatta tagcaaaaac
     3061 agctaaaatt gagaattttt attcgatttt agatatgcta taatgatcaa gacagtaaag
     3121 tttgtgacgg tggctatccg attgaaagtg aggtggtgcg tatgtgcgta cttccaaaat
     3181 cttacagaaa ggagaagcct tttgcctgtt gaaacggcgc taggattaat gattagtttc
     3241 ggttctttga tcgcaacaat catttttgga atcttagcag ttgtaaacaa caatcaaaaa
     3301 aaataaccgt cagctttagc cggtcgacga ttatttttca aaaataatat taagctggtc
     3361 accgtcttaa acggtgctgt taggggagtt gttagcgcaa ctcctctttt cgtatccatt
     3421 ataacaactt tattgatctt ttacaatcag aatggtaaat catcatcact aatatcgatt
     3481 gattgtccgc caaatggatt ttgtgaagcg ttcaatccat tgttgttagg cgtagctaca
     3541 tcccgttgtt caaaggtggt agggcttgga ctgctggacg attgatcagc gtgcgcacgg
     3601 ctttctgtcg ctgctttact ttctaatagt tggaaattat cggccacaac ttctgtcaca
     3661 tacacacgtt gtccttgttg gttatcgtag gttcgggttt gaatccgtcc aacaacgccg
     3721 atcaaactgc cctttttagc taatgtcgcc atcgtttctg ctggtttgcg ccaaatcaca
     3781 cagttaataa aatcagcttc tcgattgccg ttttgattcg tgaaatttcg gttgacagct
     3841 agggtaaaag tcgctactgc agtaccactc gcggtataac gtaaatctgg atctttggtt
     3901 aagcgtccta ctaaagttac attattgatc atctttatct ctccatttct tgattcattt
     3961 gttgtgatct tttaaactgc cgttttcttt cttgattttc aattttgatc acacttttca
     4021 ttaggtgatc aatttcgttg actgtatctg taaatgaaat ggaattcgca aacgtgttaa
     4081 ctgagcgata tttttcccac tgcttttcca aatagtcatt tcctctgatc ttatccattt
     4141 ctctttcata ataaaaggac aacggttttg tatccccacg ttttgtaaat gtatttcgaa
     4201 cacttttgta taactctttc gtatcgatat tttctagttt cggaatggta aataagtcgt
     4261 agtaatcttt tgctctcgaa ttatctgtgc caaagctgaa cgtagcactt agcttttctg
     4321 caataatttg ttctgttgga taagctggaa tctccacttt tttatcttca aacattaatt
     4381 ggtgatagtg tgtatctact tcaggataaa tagaatctcc tgttgtcacg tccattttaa
     4441 acggtacacg tactttctct aaattagcca ccaatttaat ttgaaatcca ggataaaaat
     4501 cggcttctct tgtttccttc attccttgaa tagagaaaac aatgccttct tttgtgggtt
     4561 ttctagttaa atcattaaaa atgctcttca atttttcttc agttaatttg ctatttctaa
     4621 atgtcgtatc gatatcaatt gttgtccttc tatcgattcc atattttgat cctaataaaa
     4681 agccgccttt caaaatgaag tcatttttgt actttgattc agaaatttgt tcaattagac
     4741 gatccatcat aaaatgccgt tgcactaact gtgcatttaa tcctgtactc tttgctaaat
     4801 ttcttgtttt tgctgcaaat tgtgtcggtg tcatcttttt cctccatcaa tataagcttt
     4861 gcatgtggta tttcatttct ttttctacat gtagttttgt catgtaatct cttaatttac
     4921 ttggatctct caatgggtct tccatgtact cttttaatgc ttctaatttc atttcatatt
     4981 caaattcata tctcggattc caaatatcac aaagtgtacg ctctttatca tacatagtaa
     5041 taggatttcc ttgaaaggaa gttccctcta cttttcctag ttcataaact tctttcttca
     5101 cgaaatgaaa ttgtaactgc tgctcttttg taattgaacg atcaacgtta tagccatggg
     5161 gaaaagttaa gtgaatgaat ttcggcatat tcgtagaaag ctggtaaata tctaaagtcg
     5221 tttcatgaga gaagattccc ctggaaaatc gttgctgaag ccaaaataaa tcatctattc
     5281 catgaccatc ctctacataa taaccacgat caactttgat caattttcct tgattcacca
     5341 atctttgtac agtggttgct gcgattccaa atttcctggc ttccttcata gataaatttc
     5401 catcgcgaat agccaacaat tctttcagtt tatcttgcat ttacttcccc tcccttcgtt
     5461 acaaatacac tacatcaaat atatcatagt agtttatttg taacaagatt aacgtctatc
     5521 gatctaattc gatttctttt tgaatgcgct gctggttctt taattgttgc agtcttcttt
     5581 tatttgcacg taatttctct tgttctaaac gaatttttgc tttttgattt tcgatttctt
     5641 gttgtttgtg tgttgtattt cgcataggat aaccccttca ttattttgta gtgcggcgtg
     5701 ttcttttctt atagttcgtt tttttctttt cagaaaattc aaagcctgtt gtaaagtctt
     5761 cttttaaaac gatcgttgct tcaaacgaac ctttctgacc ttttagtcct ctgactgatt
     5821 ggcttgttcg gccgttttga atgatctctt tcagttgtgt ggctgtgagt ttctttttgg
     5881 caatcgttgt ccataattta aaatcacagg cttcagacgt acatgccgcc acattttgat
     5941 aaagcaagac ttcttgttcc cgacattttg gacaaacacc taaatgacgc gttgcttcct
     6001 gtttttcact ggctgctttc acctcttgta acgttgatcc aaaatctgtt gactggattt
     6061 gtcccggtac gacttccaac aagtgagaga caaatttttg gatattagct aagaaagtag
     6121 cttgtgttcc tttctgctca ccaatcttta ataagtaact ttcccatttg gcggtcattt
     6181 cggcacttgt tagcagcggc tctttttcca cggcttggca aagtaaaatc cctttatttg
     6241 tcaccacaag cttatttttt tctaccttga tatattcctt ttgtttcaaa gtttcaataa
     6301 tatttgctcg tgttgcttct gtaccgattc cttctacttc tttcaatatg gattgtgctt
     6361 cttcgctatc cacggttttt ccagctgttt tcatcgctgt gatcaacgta ccttcggtat
     6421 aaggttgtgg tggcttcgtt tctttttcaa ggacttgtag atctaccgtc accgcttctc
     6481 cttttgtcac tttaggaagt agcggttctt cttccttttc ttttctgtcg ctctctttgc
     6541 caaacagctt tttccaacct agatctactg gtacttttcc gactgtcttt agctgaagct
     6601 gctgaacttg cgtcaagatc gttgtttctt cataggtata cttttccgca aacatggcca
     6661 ccgttgtttt taacacttgt tcataaatag cctgctgcag tggacttaat tgttcaaaag
     6721 ccgctgccgt catgaccgtc tttgtaggaa tgatcgcgta atgttcctgg actttcttat
     6781 tattcacata tcgactattc ggtgtatgaa taggagtagg aatggcttcg gcctttaaaa
     6841 agtgtttgta ttcgtctaag tgatccaaca gatacgcata ctccccttct gtaatataag
     6901 gggtatctgt tcgtgggtag cttagatatt tcccttcata caacccttgc atggcttcca
     6961 aagtatcttt ggcactagct ttcatgagct gattcatttt agattgaagg ctggatagag
     7021 aaaaaagacg tggacttgcc gcactttttt tctcggtttc aacttggtgg atcgtacctg
     7081 gctgttttcc caattgaacc cccttttctt ttaaataatc ttcaaatgct tcttgcgtcg
     7141 cttgggtttc attcggatca agcttcgcat caaatgcgcc attttgagcg attacttggg
     7201 cttttccttc aaaatatggt tctttttgga agtttctaat cttttcctgt aattgataaa
     7261 tcatgtaaag cgttggggtt tggactctgc ctaaggaaaa acttcctggt atgccttttt
     7321 gctgcaagag taaactatac agcgggctac cgttcatgcc gatcaaccaa tcggcgatct
     7381 ggcgtgcttg tgcttcttca aattttgggt aggtctcttc tgcatctcgt aactgctgga
     7441 atccctgata aatggcttct ttttctaagg agttgatcca aagtctttta aatatctttc
     7501 catgatcggc gcctgcttgt tgaatgatcg tccaagctat cagttcccct tcacgatcgc
     7561 tatctgtcgc aataataatg gtgtcggccg attgaagttg ctgtttcacc accttgaatt
     7621 gctttttctt atctttagga acatgatagt gataatgtgt agggaaaata ggcaaatgct
     7681 ctaatgacca ttgtttccaa ttttcatcgt acatatcggg cgaatctaac tcgactaaat
     7741 gaccaaaacc atacgtgatc acagtttctc ctgaaaacaa tcgatcttgg atctcaaaat
     7801 acccatcttt tcgggtagct ttagaaaagc tatcggcata agccttcgct tgtgacggtt
     7861 tttctgctaa aataacggtt ttcatcatga cgatttcact ccctttcttg ctgtctacct
     7921 tgttgaaaca acatggcacg tttttgcttt ctgatctctt gttggtagat ttcatgcttc
     7981 aattgatcat tccaatcctt ttttacttca ggtacaaatt catgttcatg atttttatag
     8041 cctttgtagt attgttctgc tttataagag aaaagaatct gagcattaat atcccctttt
     8101 tcttgataaa aacgatcaaa atcgtacgta ccatccacgc gttcacaagc ttttaattct
     8161 tttgcgactt ctctacaaat atctttcaaa ttaatagctt ctattgaatc gttaacagaa
     8221 agctgctgac caaaagacgc atttactttg tgagcggaaa ccaattggtt cgttaattgc
     8281 agattgtttt ctgtcttgtg tatggccata atagcttctg gtgtgatctt ttcttctttt
     8341 gcgacttctt cataaatgac tttgtattct tttggcactt gccaatcatt agcgattcct
     8401 cgttcacaac gtactttctg attcgtaaat ggatcaactg cgcccattaa ttgttctttt
     8461 tcgtagaaaa tatgtcctgc acggtcatta tccacacaaa tctcgatgga ttgaggaaag
     8521 gcttgtttct ttctcaattc agatactgcc tcaccaaaat aatgactgat cacatggtgt
     8581 tttaaccctt ccatcgaaac taaccacgta tcgtttaact gatcacgatt tagtgcagca
     8641 tagctaagta aatcaatact actctcaaaa aattttaact gcttagggtc accgatcttt
     8701 aggttaaagc catgattcgc tgtcgaattt ttatctatat gcttatatgt gcctctctca
     8761 ccaaaacgtt cataatcaat gaacgttcct tgataactcg cccctactgt ctcatttgtc
     8821 tgtggatctt tccataaaaa ggcgaccgca tgatctaaac gatcgtctcg aactaagaaa
     8881 ttttgccgtt catgttttgg ctttagttcg gcaatcaacc cttctttttc tgcataatca
     8941 atcagccact ctggtagttt tcgttcttcc ttcagataat tccttccttt ttctgtgagt
     9001 ggttgataat caacggggtg atctttgtac ttttctgtct caaaatgaag attttccact
     9061 cgttctgttt tgtcttcatt ctttagaata aaatcagtgg ctgctttaaa ttgttgctta
     9121 gggtcttgga tctctttgtc tatgaaaaaa agtttagcta attcaataat gtcgccacta
     9181 atattttgac tgttccaata aaaacgttgt tttcttcgat caaaaacaaa tgaatcatgg
     9241 tcttctaagc gatagtctcg tcctttattg actacagcca ttccattgtt tcgagcaaaa
     9301 tccactatat cgatctgttt acatcgatca ataagactca tttttttctc ggtcatttta
     9361 aaacctccca aaagaaaaaa gactatccgt ttttcagata gtctttttta cttgttttat
     9421 acacgttctt tttgtgacaa ttgttgttca taatagattt cattttttcg ttttgccact
     9481 tctgacactg tctcaagtga aaatcggaat gaatttccta gcgcaaattc ttctgaaggt
     9541 aacgccgttt ggatctcttt tccataactt tttcctttta aaattacttg attatgctgt
     9601 ggatcttcaa taaacgccgc atccttcaaa tgtaacgttc gacccgtatc ttcttccatt
     9661 actttcccgt ctttcttgtc gatccaaagt gaaccaacag gaacaatatt cttttgttct
     9721 gccgtttcta cagtattttt atgctcctgc ttcacttctg ctggttggat ctctggttct
     9781 ctttctctgt attgattgat ttcattagaa atggtttgaa tgaattcatt tgcgacacgg
     9841 ctgacatcat tcatgatttt cccacgttcc gcagcttcct tatcatccaa cgtctgacca
     9901 ttcttcgtcc atgctgcaat ataaggaata gctttctccg aagtgtccat tccaaaatgt
     9961 ttgcacacaa tataagaagt catttctgcc tgcaattctt tagttgcggt atctaattct
    10021 gaaaatttgt gcattcttgc atgggctaat tcatgaatac ttgtagctaa atgctgcgta
    10081 ggagtatttc gagtattcat gacgatttct tcttcgccag taaattcgtt acgaacatat
    10141 gccccccgtg cttgtcctaa ttctctttga tagacttcac tttctgtcat atctcgaatt
    10201 tgtattccaa tattgttggc taccgcttga atcccagctt gaagttctga ctgtcctttt
    10261 ttatccaatt gaaaatcaaa gacacggttc ggaaagattt tcggatagtc ttcaggtttt
    10321 aactgtgttt gtgtgatatc aaacactttt tcaatcgtgt agtaaggttt tttgatcact
    10381 tcgatttgac cactttcgat tcgtttttta tcttctggtg tagcttctgt ttctcgaaca
    10441 aagccacgtc ctttctcata aaatcctttg accgtttttc tgacaaagat cttgttccct
    10501 tgttcccctt ttttgatata gattccttct tttttcaacg cagcacgacc taaacaagcc
    10561 gttgcacctg gaaattgttc ctcaatcaat aattgatttc taaaggatcg ttctggaaaa
    10621 cgagacataa aattcaaaag ttctttcacg tcttcaggag aatgggtatg ctttttcacc
    10681 gcatccagtg cctgttgcga aagggatttg acttcttgct taatctcttc tgccgttcgt
    10741 ttttgatagc ccttttgttt tgtttttgtt tctttcgact gcatttgttc tttcgaaaca
    10801 ggttcatttt tgaccgtttg tttttgttcc ttttcaggta cttgttcgca caattttgct
    10861 aactctaaat agatgggttg attactgttg acgccatccc ctagatccat tcggatctga
    10921 tcgactactt ctccgttctt cacttcttca aaatagaatt tataataacc tgcttctgcg
    10981 gcgtattggt tatccaacgt tttgagttga tcgatcaatt ctaatgttac gatctcccca
    11041 ccataacttt tcaatccttt ttctggatca ttttcgttga attctactcg atagaaatga
    11101 tcgttttctt tctccttcaa agaatcagtt ggcgcagcct cttcaagtaa ttcacgaaca
    11161 gctgcttttt cctcttccga cgtggcttgg tatttttttg attcctcaaa tcccccgttt
    11221 tcattcagtt ctaaagcaac ttttcgatag tctttcaaaa aataaatggt tggatttttt
    11281 ttccattgcg tcactcgtcc gctgtcaata attgcttgta cttcaggaag aagattttct
    11341 tttccttttt ctgatctttc tttcagctgt tctaatgttt ccaatttccg ttggtatttt
    11401 cggatcgtag cggctgaaaa agtcgattca cctttttcaa agcgttcgat ttcttctttg
    11461 attcttggaa tattttcgat tgtcatatcc aggccgccgt acttgttata gcccatttcc
    11521 tttagctctt cttgtctttc aagtttttct acctgttttt cttgttgctt gatctcttct
    11581 ctcagactat gcactttgtt ttctaaacgt tcttgttgcc taaaccaaga atgcccattt
    11641 ctcttatcgt tcattggctg accgttggtc tttctctggt gttcaaacat cttctcaatt
    11701 tgttcgttta attcattttg caatcgtact aattttcttt tggattttcc caagcgacta
    11761 ttgtctagtg gttctttttc ttctgccatg tcc
//
LOCUS       DAZKAS010000084        11770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000084 DAZKAS010000000
VERSION     DAZKAS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.086"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..650)
                     /locus_tag="ACG8H7_001880"
     CDS             complement(69..650)
                     /locus_tag="ACG8H7_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8983960.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(833..1459)
                     /locus_tag="ACG8H7_001881"
     CDS             complement(833..1459)
                     /locus_tag="ACG8H7_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP8983961.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1481..2812)
                     /locus_tag="ACG8H7_001882"
     CDS             complement(1481..2812)
                     /locus_tag="ACG8H7_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFP8983962.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2967..3368)
                     /locus_tag="ACG8H7_001883"
     CDS             complement(2967..3368)
                     /locus_tag="ACG8H7_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFP8983963.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3454..3660)
                     /locus_tag="ACG8H7_001884"
     CDS             complement(3454..3660)
                     /locus_tag="ACG8H7_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8983964.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3755..5257)
                     /locus_tag="ACG8H7_001885"
     CDS             complement(3755..5257)
                     /locus_tag="ACG8H7_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFP8983965.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5275..5730)
                     /gene="argR"
                     /locus_tag="ACG8H7_001886"
     CDS             complement(5275..5730)
                     /gene="argR"
                     /locus_tag="ACG8H7_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP8983966.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5828..6445)
                     /locus_tag="ACG8H7_001887"
     CDS             complement(5828..6445)
                     /locus_tag="ACG8H7_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP8983967.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6666..8039
                     /locus_tag="ACG8H7_001888"
     CDS             6666..8039
                     /locus_tag="ACG8H7_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8983968.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8114..9226
                     /locus_tag="ACG8H7_001889"
     CDS             8114..9226
                     /locus_tag="ACG8H7_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8983969.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9298..10935)
                     /locus_tag="ACG8H7_001890"
     CDS             complement(9298..10935)
                     /locus_tag="ACG8H7_001890"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP8983970.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11103..11711)
                     /locus_tag="ACG8H7_001891"
     CDS             complement(11103..11711)
                     /locus_tag="ACG8H7_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP8983971.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3090 a         2483 c         2132 g         4065 t
ORIGIN      
        1 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
       61 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
      121 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
      181 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
      241 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
      301 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
      361 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
      421 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
      481 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
      541 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
      601 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
      661 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
      721 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
      781 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
      841 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
      901 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
      961 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     1021 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     1081 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     1141 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     1201 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     1261 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     1321 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     1381 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     1441 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     1501 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     1561 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     1621 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     1681 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     1741 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     1801 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     1861 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     1921 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     1981 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     2041 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     2101 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     2161 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     2221 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     2281 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     2341 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     2401 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     2461 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     2521 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     2581 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     2641 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     2701 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     2761 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     2821 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     2881 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     2941 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     3001 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     3061 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     3121 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     3181 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     3241 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     3301 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     3361 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     3421 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     3481 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     3541 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     3601 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     3661 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     3721 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     3781 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     3841 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     3901 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     3961 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     4021 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     4081 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     4141 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     4201 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     4261 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     4321 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     4381 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     4441 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     4501 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     4561 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     4621 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     4681 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     4741 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     4801 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     4861 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     4921 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     4981 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     5041 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     5101 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     5161 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     5221 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     5281 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     5341 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     5401 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     5461 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     5521 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     5581 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     5641 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     5701 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     5761 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     5821 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     5881 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     5941 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     6001 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     6061 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     6121 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     6181 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     6241 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     6301 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     6361 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     6421 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     6481 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     6541 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     6601 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     6661 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     6721 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     6781 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     6841 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     6901 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     6961 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     7021 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     7081 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     7141 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     7201 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     7261 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     7321 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     7381 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     7441 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     7501 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     7561 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     7621 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     7681 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     7741 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     7801 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     7861 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     7921 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     7981 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     8041 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
     8101 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
     8161 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
     8221 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
     8281 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
     8341 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
     8401 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
     8461 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
     8521 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
     8581 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
     8641 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
     8701 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
     8761 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
     8821 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
     8881 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
     8941 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
     9001 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
     9061 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
     9121 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
     9181 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
     9241 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
     9301 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
     9361 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
     9421 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
     9481 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
     9541 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
     9601 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
     9661 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
     9721 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
     9781 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
     9841 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
     9901 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
     9961 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    10021 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    10081 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    10141 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    10201 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    10261 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    10321 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    10381 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    10441 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    10501 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    10561 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    10621 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    10681 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    10741 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    10801 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    10861 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    10921 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    10981 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    11041 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    11101 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    11161 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    11221 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    11281 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    11341 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    11401 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    11461 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    11521 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    11581 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    11641 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    11701 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    11761 ttatcattta
//
LOCUS       DAZKAS010000085        11639 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000085 DAZKAS010000000
VERSION     DAZKAS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.087"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8H7_001892"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG8H7_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP8983972.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG8H7_001893"
     CDS             complement(322..891)
                     /locus_tag="ACG8H7_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFP8983973.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG8H7_001894"
     CDS             1077..1979
                     /locus_tag="ACG8H7_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP8983974.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8H7_001895"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG8H7_001895"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP8983975.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8H7_001896"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG8H7_001896"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP8983976.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8H7_001897"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG8H7_001897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP8983977.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACG8H7_001898"
     CDS             8547..8732
                     /locus_tag="ACG8H7_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFP8983978.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACG8H7_001899"
     CDS             complement(8794..9990)
                     /locus_tag="ACG8H7_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP8983979.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACG8H7_001900"
     CDS             complement(10160..11596)
                     /locus_tag="ACG8H7_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFP8983980.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3431 a         2485 c         2043 g         3680 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactata
//
LOCUS       DAZKAS010000086        11498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000086 DAZKAS010000000
VERSION     DAZKAS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.088"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..645)
                     /locus_tag="ACG8H7_001901"
     CDS             complement(100..645)
                     /locus_tag="ACG8H7_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP8983981.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(657..1349)
                     /locus_tag="ACG8H7_001902"
     CDS             complement(657..1349)
                     /locus_tag="ACG8H7_001902"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP8983982.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1368..1652)
                     /gene="macP"
                     /locus_tag="ACG8H7_001903"
     CDS             complement(1368..1652)
                     /gene="macP"
                     /locus_tag="ACG8H7_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFP8983983.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1665..2228)
                     /locus_tag="ACG8H7_001904"
     CDS             complement(1665..2228)
                     /locus_tag="ACG8H7_001904"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFP8983984.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2456..3118
                     /locus_tag="ACG8H7_001905"
     CDS             2456..3118
                     /locus_tag="ACG8H7_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFP8983985.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3242..4438
                     /locus_tag="ACG8H7_001906"
     CDS             3242..4438
                     /locus_tag="ACG8H7_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFP8983986.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4539..5915)
                     /locus_tag="ACG8H7_001907"
     CDS             complement(4539..5915)
                     /locus_tag="ACG8H7_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8983987.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5952..6818)
                     /locus_tag="ACG8H7_001908"
     CDS             complement(5952..6818)
                     /locus_tag="ACG8H7_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFP8983988.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6907..7932)
                     /locus_tag="ACG8H7_001909"
     CDS             complement(6907..7932)
                     /locus_tag="ACG8H7_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8983989.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7950..8624)
                     /locus_tag="ACG8H7_001910"
     CDS             complement(7950..8624)
                     /locus_tag="ACG8H7_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8983990.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8808..9701)
                     /locus_tag="ACG8H7_001911"
     CDS             complement(8808..9701)
                     /locus_tag="ACG8H7_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFP8983991.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9775..11472)
                     /locus_tag="ACG8H7_001912"
     CDS             complement(9775..11472)
                     /locus_tag="ACG8H7_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFP8983992.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3410 a         2522 c         1972 g         3594 t
ORIGIN      
        1 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
       61 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
      121 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc
      181 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
      241 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
      301 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
      361 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
      421 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
      481 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
      541 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
      601 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
      661 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
      721 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
      781 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
      841 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
      901 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
      961 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
     1021 cctggtttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
     1081 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
     1141 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
     1201 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
     1261 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
     1321 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
     1381 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
     1441 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
     1501 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
     1561 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
     1621 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
     1681 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
     1741 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
     1801 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
     1861 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
     1921 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
     1981 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
     2041 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccacc aggatgaaat
     2101 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
     2161 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
     2221 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
     2281 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
     2341 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
     2401 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
     2461 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
     2521 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
     2581 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
     2641 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
     2701 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
     2761 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
     2821 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
     2881 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
     2941 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
     3001 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
     3061 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
     3121 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
     3181 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
     3241 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
     3301 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
     3361 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
     3421 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
     3481 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
     3541 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
     3601 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
     3661 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
     3721 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
     3781 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
     3841 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
     3901 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
     3961 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
     4021 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
     4081 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
     4141 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
     4201 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
     4261 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
     4321 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
     4381 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
     4441 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
     4501 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
     4561 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
     4621 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt
     4681 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
     4741 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
     4801 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
     4861 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
     4921 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
     4981 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
     5041 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
     5101 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
     5161 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
     5221 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
     5281 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
     5341 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
     5401 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
     5461 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
     5521 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
     5581 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
     5641 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
     5701 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
     5761 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
     5821 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
     5881 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
     5941 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
     6001 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
     6061 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
     6121 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
     6181 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
     6241 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
     6301 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
     6361 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
     6421 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
     6481 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
     6541 ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca
     6601 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
     6661 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
     6721 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
     6781 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
     6841 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
     6901 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
     6961 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
     7021 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
     7081 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
     7141 aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc
     7201 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
     7261 atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa
     7321 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat
     7381 gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc
     7441 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
     7501 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
     7561 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
     7621 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc
     7681 actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
     7741 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
     7801 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
     7861 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
     7921 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
     7981 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
     8041 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
     8101 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
     8161 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
     8221 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
     8281 aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct
     8341 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
     8401 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
     8461 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
     8521 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
     8581 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
     8641 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
     8701 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct
     8761 cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt
     8821 ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc
     8881 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
     8941 tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg
     9001 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta
     9061 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
     9121 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
     9181 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
     9241 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
     9301 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc
     9361 atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa
     9421 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
     9481 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
     9541 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
     9601 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
     9661 ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt
     9721 aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac
     9781 ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc
     9841 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
     9901 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
     9961 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc
    10021 ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa
    10081 gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct
    10141 atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc
    10201 agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc
    10261 atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc
    10321 ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc
    10381 atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg
    10441 tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat
    10501 cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat
    10561 cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt
    10621 atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa
    10681 ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata
    10741 agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc
    10801 ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc
    10861 tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat
    10921 catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata
    10981 agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac
    11041 ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg
    11101 atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga
    11161 attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac
    11221 ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt
    11281 agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa
    11341 tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttggcta gttttccgtt
    11401 cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat
    11461 ttttttattc atttttttct ccccgttctt tttttatt
//
LOCUS       DAZKAS010000087        11128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000087 DAZKAS010000000
VERSION     DAZKAS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.089"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="ACG8H7_001913"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="ACG8H7_001913"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP8983993.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="ACG8H7_001914"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="ACG8H7_001914"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP8983994.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /gene="dapA"
                     /locus_tag="ACG8H7_001915"
     CDS             2139..3020
                     /gene="dapA"
                     /locus_tag="ACG8H7_001915"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP8983995.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /gene="dapB"
                     /locus_tag="ACG8H7_001916"
     CDS             3017..3733
                     /gene="dapB"
                     /locus_tag="ACG8H7_001916"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP8983996.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="ACG8H7_001917"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="ACG8H7_001917"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFP8983997.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="ACG8H7_001918"
     CDS             4450..5451
                     /locus_tag="ACG8H7_001918"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP8983998.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="ACG8H7_001919"
     CDS             5448..6809
                     /locus_tag="ACG8H7_001919"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFP8983999.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="ACG8H7_001920"
     CDS             6769..7920
                     /locus_tag="ACG8H7_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFP8984000.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="ACG8H7_001921"
     CDS             7895..9064
                     /locus_tag="ACG8H7_001921"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFP8984001.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="ACG8H7_001922"
     CDS             9239..10618
                     /locus_tag="ACG8H7_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8984002.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="ACG8H7_001923"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="ACG8H7_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3701 a         1946 c         2495 g         2986 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcatacagtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgcaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAZKAS010000088        10724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000088 DAZKAS010000000
VERSION     DAZKAS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.090"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..1089)
                     /locus_tag="ACG8H7_001924"
     CDS             complement(241..1089)
                     /locus_tag="ACG8H7_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8984003.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1253..2155
                     /gene="gnd"
                     /locus_tag="ACG8H7_001925"
     CDS             1253..2155
                     /gene="gnd"
                     /locus_tag="ACG8H7_001925"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFP8984004.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="ACG8H7_001926"
     CDS             2157..3158
                     /locus_tag="ACG8H7_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFP8984005.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="ACG8H7_001927"
     CDS             3155..3439
                     /locus_tag="ACG8H7_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP8984006.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="ACG8H7_001928"
     CDS             3472..4269
                     /locus_tag="ACG8H7_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP8984007.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="ACG8H7_001929"
     CDS             4283..5116
                     /locus_tag="ACG8H7_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8984008.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="ACG8H7_001930"
     CDS             5134..5991
                     /locus_tag="ACG8H7_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8984009.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="ACG8H7_001931"
     CDS             6013..6522
                     /locus_tag="ACG8H7_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP8984010.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="ACG8H7_001932"
     CDS             6559..6960
                     /locus_tag="ACG8H7_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984011.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="ACG8H7_001933"
     CDS             7072..7425
                     /locus_tag="ACG8H7_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984012.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="ACG8H7_001934"
     CDS             7459..7902
                     /locus_tag="ACG8H7_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8984013.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="ACG8H7_001935"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="ACG8H7_001935"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP8984014.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="ACG8H7_001936"
     CDS             8997..9710
                     /locus_tag="ACG8H7_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8984015.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="ACG8H7_001937"
     CDS             complement(9917..10066)
                     /locus_tag="ACG8H7_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984016.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="ACG8H7_001938"
     CDS             10462..10662
                     /locus_tag="ACG8H7_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8984017.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1695 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attc
//
LOCUS       DAZKAS010000089        10590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000089 DAZKAS010000000
VERSION     DAZKAS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.091"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(777..2408)
                     /locus_tag="ACG8H7_001939"
     CDS             complement(777..2408)
                     /locus_tag="ACG8H7_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFP8984018.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="ACG8H7_001940"
     CDS             complement(2422..4095)
                     /locus_tag="ACG8H7_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFP8984019.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="ACG8H7_001941"
     CDS             complement(4108..4425)
                     /locus_tag="ACG8H7_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP8984020.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="ACG8H7_001942"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="ACG8H7_001942"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFP8984021.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="ACG8H7_001943"
     CDS             6087..6872
                     /locus_tag="ACG8H7_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP8984022.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="ACG8H7_001944"
     CDS             6875..7567
                     /locus_tag="ACG8H7_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8984023.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="ACG8H7_001945"
     CDS             complement(7841..8212)
                     /locus_tag="ACG8H7_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFP8984024.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8612..8842)
                     /locus_tag="ACG8H7_001946"
     CDS             complement(8612..8842)
                     /locus_tag="ACG8H7_001946"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFP8984025.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(8936..9274)
                     /locus_tag="ACG8H7_001947"
     CDS             complement(8936..9274)
                     /locus_tag="ACG8H7_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984026.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(10202..10318)
                     /locus_tag="ACG8H7_001948"
     CDS             complement(10202..10318)
                     /locus_tag="ACG8H7_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFP8984027.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         3306 a         2217 c         1728 g         3339 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     8401 caataagcta attaagaaac aaactttaga aactctagaa gaacttctga atttatatag
     8461 aagtgtgtag ctacttacta aatatacaaa agttaaaata aaatactctg gatactcttt
     8521 atactgcatt aaagttagta atattaacag caaagatatc gaaaaaacaa taacataata
     8581 tgtgtcaaaa tttttcataa taagaactcc cttactgttg catactaaca taatccaaaa
     8641 actcttctac ttcagctaaa gacattccac tttctttagt ccaaaaaaca gttttttcta
     8701 tcgatccttc tctgtaaact gtaaatgtcg gagtcgagtt aattgagtag cgactcttga
     8761 attctttcca ctcattttca tcttctctaa cttttgctac attcacgtaa taaatatttt
     8821 cttttccctt ttctaccagc atatcgaata gcttcctgct tttgatgatc gatttcttgt
     8881 gctacatcta gtaaattttc atcaatttct atatcataaa gtatttcact agtgtctaca
     8941 tccacaatat atacttttta tcagtagcaa tttttgagta atccgttaaa atgatctcag
     9001 tctcgtcact ttcatcagta taaatatacg ttaacaatga actcaccttt tcaatcggtg
     9061 ttagctctaa gcctactgta ttttttacta ttgagtcatt atttaccacc ataacttcgt
     9121 actgtatata ccatcctatt tggtttccat caatatcagt aattggctca ctagctaatg
     9181 gagtctccgg aacaatattt tcctgatatt tcttaaactc ttctgtagca cgaaaaaggt
     9241 tatcatgttc ctcacttgaa actggttgag tcatagaaaa tgttacttgg ctaaacagag
     9301 ggaaaataaa cactacaatc aacaaagtat aaaaaaaagc ccttcttttt cattttttat
     9361 cttccaatca caaattgaaa aacttattcc tgcaactttg agtctagtat cacaatcgac
     9421 tgactacaac tattcattta cattctactt taagttcctc cttaaccatt aattttatta
     9481 aagatacttg taatagtgat tctcacaaaa tttcatgaaa aatactttat tactgtatct
     9541 aaaattccaa tacacaactt atcgactatt atctgaaaac caaaacttat tattccttga
     9601 tgttattcta gcaatagtaa ctgctttttt atttttttgc atattttttc ggcgttactt
     9661 tttaaagtta actttttcga caaatagaaa gagatacgaa tcactgattt ttttgaagaa
     9721 aagaaatgga gtgagctgaa cgttagagat attgggagct ttcgtaactg cttattaaac
     9781 gataatggtt atttgcaacc gtattgctcg cttctacatg aaatatttca tgaaatattt
     9841 cgcaaaacct ttgattatgt agttacgttt ccattcttga ctaaaaatgt ttcaaaaacg
     9901 aacaaaggtg ttaaaaggta aagtgatcaa tactactggt caaaataaga ggttgaaagg
     9961 gaattgaaaa ataactacag ataatacttt tataaacgta cgtgctttgt ggttctgtag
    10021 ttaactatat aactggaatt agggaatggt atcaatttga aaagaaagat tctaccaatt
    10081 tatcatatgt taaaaatgga aaatcaacag cagttctttc ggaaacctta cacaaaaacc
    10141 gattattgaa aatagccgaa aaacgttttt cggctatttt tttaattaag tttccttctt
    10201 cttacctttt cccactattc cgtttcacat ctttgatgat cttcagcgca cgttttcgtg
    10261 ttttgatatt tgggcttttc aagcggcggt atgcacttga gatatctgtt ttttccatat
    10321 ggacgaccca ctttcttctt tgattgtagt catgctaaaa aacacaaatc gaaacaatta
    10381 cgatttgtgt tttaaatggt atcaaaagtt aaaatgaaag tcaacttgtt aaaacagtcg
    10441 atcagactag ctgattgact cctatctctg gaaataaatt ttcattatta cgttttagct
    10501 acctaaaaaa ggccctcttg tccgttaact ttaatatcta ttgttatagc tagtttacct
    10561 attgaacgta ctgtgtgttc gatagaacac
//
LOCUS       DAZKAS010000090        10532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000090 DAZKAS010000000
VERSION     DAZKAS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.092"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACG8H7_001949"
     CDS             complement(25..162)
                     /locus_tag="ACG8H7_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFP8984028.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG8H7_001950"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG8H7_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP8984029.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACG8H7_001951"
     CDS             complement(1599..1853)
                     /locus_tag="ACG8H7_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984030.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACG8H7_001952"
     CDS             complement(1878..3020)
                     /locus_tag="ACG8H7_001952"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP8984031.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACG8H7_001953"
     CDS             complement(3080..4432)
                     /locus_tag="ACG8H7_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP8984032.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACG8H7_001954"
     CDS             complement(4503..4847)
                     /locus_tag="ACG8H7_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP8984033.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACG8H7_001955"
     CDS             complement(4857..5231)
                     /locus_tag="ACG8H7_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP8984034.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACG8H7_001956"
     CDS             complement(5244..5558)
                     /locus_tag="ACG8H7_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP8984035.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG8H7_001957"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG8H7_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACG8H7_001958"
     CDS             complement(8976..9638)
                     /locus_tag="ACG8H7_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984036.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ACG8H7_001959"
     CDS             10255..10485
                     /locus_tag="ACG8H7_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984037.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DAZKAS010000091        10518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000091 DAZKAS010000000
VERSION     DAZKAS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.093"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..1137
                     /locus_tag="ACG8H7_001960"
     CDS             19..1137
                     /locus_tag="ACG8H7_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP8984038.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1265..2641
                     /locus_tag="ACG8H7_001961"
     CDS             1265..2641
                     /locus_tag="ACG8H7_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP8984039.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3042..4622
                     /locus_tag="ACG8H7_001962"
     CDS             3042..4622
                     /locus_tag="ACG8H7_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP8984040.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4820..5043
                     /locus_tag="ACG8H7_001963"
                     /pseudo
     CDS             4820..5043
                     /locus_tag="ACG8H7_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5248..5568)
                     /locus_tag="ACG8H7_001964"
     CDS             complement(5248..5568)
                     /locus_tag="ACG8H7_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFP8984041.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5716..7950)
                     /locus_tag="ACG8H7_001965"
     CDS             complement(5716..7950)
                     /locus_tag="ACG8H7_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8984042.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8429..8695
                     /locus_tag="ACG8H7_001966"
     CDS             8429..8695
                     /locus_tag="ACG8H7_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFP8984043.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8695..10422
                     /gene="ptsP"
                     /locus_tag="ACG8H7_001967"
     CDS             8695..10422
                     /gene="ptsP"
                     /locus_tag="ACG8H7_001967"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP8984044.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3348 a         1836 c         2152 g         3182 t
ORIGIN      
        1 aagaaaggag gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg
       61 cttgaattat tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc
      121 gaaccgatcg ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt
      181 tactatttat tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg
      241 gctgtcgcaa ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc
      301 atcccaagtc tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa
      361 caggtagaaa gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc
      421 aataaatatt ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt
      481 ggagtatcca atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc
      541 attacagcac cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac
      601 atccaacagt ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg
      661 aataaaacat tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc
      721 ttcacatttt taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt
      781 gcgggattga caaacctaat tccatattta ggaccttatc taggactagc acctgcattt
      841 ctagtaacag tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc
      901 gtccaacagt tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc
      961 catccattaa cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt
     1021 atcttcttag gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa
     1081 atcgtcaaag aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta
     1141 gttttagtac acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg
     1201 ctatagtgcc acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc
     1261 gattatgaat gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt
     1321 tttgacattg atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa
     1381 gaatcgaatc gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat
     1441 attgcagaat ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat
     1501 tttgtctggt gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg
     1561 cggcacagcg gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc
     1621 gattgttttt ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc
     1681 aaagttcact tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct
     1741 gctttctgct tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga
     1801 ttcaaaaatg accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa
     1861 tgaagaatta gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt
     1921 catggtccca cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt
     1981 gaatgaagtt ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa
     2041 agtagtcgga atccttcata caaagaattt actaaaagct gcccataaat tcggctttga
     2101 taatttagat atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat
     2161 tgatgatctg ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga
     2221 atatggcggc gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga
     2281 aatcgatgat gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata
     2341 tatcatccaa ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca
     2401 tatgagtgat gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga
     2461 tgaaggcgaa aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga
     2521 aggttctcga atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga
     2581 acctgaagaa gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata
     2641 attggaaact tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt
     2701 ttagtcagct gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga
     2761 atccatgcta tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc
     2821 attaagtaat aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct
     2881 tattgctgat aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc
     2941 ttttgtcaca gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag
     3001 agaaaatata aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa
     3061 agcaacaagt ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa
     3121 caacgattac agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa
     3181 aaggcaaaaa aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg
     3241 ggatttctgt tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct
     3301 tggatacacc aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg
     3361 atagtgcggt catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat
     3421 tccaagttgt aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg
     3481 atggtcgtga accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt
     3541 atccgatgaa ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc
     3601 ataatcgtgt agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg
     3661 atgaagatgg actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag
     3721 tcttagaaga agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg
     3781 ctcgtggcga tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga
     3841 cttttttaga gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg
     3901 atcagatggt aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa
     3961 atatgaatcc agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg
     4021 aacgaggaat ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca
     4081 cacaatttat ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg
     4141 gtgtatatga tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag
     4201 ttgcttatga agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa
     4261 atgtcatgaa acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga
     4321 ttcagcttta taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac
     4381 aattcgaagt attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc
     4441 caatgggaca caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt
     4501 caagtcgcaa tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc
     4561 aatttgcaga acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt
     4621 aaagaagtct tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag
     4681 cctctcatat tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt
     4741 aaaatcatag aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa
     4801 agaaaggaga gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa
     4861 aaagaagaga tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag
     4921 caaattgcac aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac
     4981 acgcgtagaa ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc
     5041 tagaaagaaa aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac
     5101 aaaaatttga aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct
     5161 ttcgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc
     5221 tctttaatta ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt
     5281 tggaatcgag atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta
     5341 aacggcgtct ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg
     5401 gattttacgt ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac
     5461 aatagtggta tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg
     5521 gtttaataca agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc
     5581 ttttatgtat ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca
     5641 gtattttgac ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac
     5701 gctataaaac tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg
     5761 tttagaagta acgatgattt ttccatcttt atccagtttt gctttcaact catggataga
     5821 agggtgatca agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg
     5881 aagaggacga gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt
     5941 cgtaggaact tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat
     6001 tagtgtgacg attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc
     6061 aaaacggttc agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt
     6121 aacaccttcg cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt
     6181 acttgtcata atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa
     6241 tcgaccatca tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac
     6301 ttcatctaac aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc
     6361 ttcttcgtat ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc
     6421 catatattca gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc
     6481 aagttgtttc gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc
     6541 gattggacga ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg
     6601 atctacagcg ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag
     6661 ttgtgtctgt tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat
     6721 tttttccata tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt
     6781 cattttttga agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc
     6841 tcgagaagct aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg
     6901 atcaacgatt tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc
     6961 tttatccggc aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat
     7021 ggcctcatca gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa
     7081 aatactgatc gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc
     7141 taaagcagcg tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg
     7201 caattctcct cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc
     7261 agcagaacca gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc
     7321 ttgacggatc tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt
     7381 accttggact aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg
     7441 tacatcacca tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc
     7501 tggttctcca atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg
     7561 tttgatttcg tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt
     7621 gatattgatt ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc
     7681 attgaagttg ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg
     7741 ttgcatttgg cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc
     7801 attttgggcc atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct
     7861 ctgacagtaa tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc
     7921 ctcattcttg ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg
     7981 tacatccata ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa
     8041 gagatttatg aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta
     8101 ttatactaaa aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca
     8161 aaggaaagaa tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg
     8221 tttgttataa aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta
     8281 tctgaaattg aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga
     8341 aagggtggca tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac
     8401 aacactcact taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga
     8461 aactgggatc cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc
     8521 tgatgtaaac ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat
     8581 gtctttaggt gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc
     8641 tgatgcaatg gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt
     8701 gaaatgctaa aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta
     8761 gttcaaccgg atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct
     8821 cgtttggatg ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca
     8881 gcgcaaagct taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca
     8941 gatccagaaa tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa
     9001 tcagcgctaa aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat
     9061 gcttatatgc aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac
     9121 ttattaggcg ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca
     9181 catgacttga ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta
     9241 acagacattg gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca
     9301 gcaatcgttg ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc
     9361 aacgggatcg aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa
     9421 aaagcaggtg cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa
     9481 actgtcacag ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat
     9541 ctagttggtg tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg
     9601 tatatggatt ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta
     9661 ttagaaggta tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa
     9721 gaactaccat atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg
     9781 cgtatcagtt tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt
     9841 cgtgcatctg ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa
     9901 ttccgcgcag caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag
     9961 gaagtatctg atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta
    10021 gcagacaagt ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa
    10081 tacacaatgg cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac
    10141 ccatcaattt tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg
    10201 gcaggtatgt gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg
    10261 ggtctagatg agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa
    10321 cgcttggaca cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg
    10381 atggaagaag tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg
    10441 gtgtggcttg taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt
    10501 cagtaagaat agacaaaa
//
LOCUS       DAZKAS010000092        10489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000092 DAZKAS010000000
VERSION     DAZKAS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.094"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..692)
                     /locus_tag="ACG8H7_001968"
     CDS             complement(207..692)
                     /locus_tag="ACG8H7_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFP8984045.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    782..1127
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1172..1813
                     /locus_tag="ACG8H7_001969"
     CDS             1172..1813
                     /locus_tag="ACG8H7_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP8984046.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1832..2467
                     /locus_tag="ACG8H7_001970"
     CDS             1832..2467
                     /locus_tag="ACG8H7_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP8984047.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2485..3300
                     /locus_tag="ACG8H7_001971"
     CDS             2485..3300
                     /locus_tag="ACG8H7_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8984048.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3619..5055
                     /locus_tag="ACG8H7_001972"
     CDS             3619..5055
                     /locus_tag="ACG8H7_001972"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8984049.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5244..7691
                     /locus_tag="ACG8H7_001973"
     CDS             5244..7691
                     /locus_tag="ACG8H7_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP8984050.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7921..8988
                     /gene="rlmN"
                     /locus_tag="ACG8H7_001974"
     CDS             7921..8988
                     /gene="rlmN"
                     /locus_tag="ACG8H7_001974"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFP8984051.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8978..9184
                     /locus_tag="ACG8H7_001975"
     CDS             8978..9184
                     /locus_tag="ACG8H7_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984052.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9181..10284)
                     /locus_tag="ACG8H7_001976"
     CDS             complement(9181..10284)
                     /locus_tag="ACG8H7_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP8984053.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3465 a         1794 c         2282 g         2948 t
ORIGIN      
        1 gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg
       61 gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt
      121 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
      181 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
      241 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
      301 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
      361 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
      421 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
      481 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
      541 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
      601 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
      661 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
      721 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
      781 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
      841 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
      901 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
      961 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
     1021 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
     1081 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     1141 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     1201 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     1261 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     1321 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     1381 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     1441 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     1501 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     1561 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     1621 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     1681 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     1741 ctatcttaca ttgactggta ttctaacaat cataatgaaa caagttgaag gaaaattaaa
     1801 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     1861 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     1921 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     1981 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     2041 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     2101 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     2161 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     2221 ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc
     2281 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     2341 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     2401 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     2461 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     2521 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     2581 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     2641 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     2701 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     2761 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     2821 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     2881 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     2941 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     3001 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     3061 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     3121 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     3181 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     3241 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     3301 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     3361 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     3421 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     3481 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     3541 attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     3601 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     3661 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     3721 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     3781 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     3841 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     3901 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     3961 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     4021 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     4081 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     4141 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     4201 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     4261 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     4321 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     4381 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     4441 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     4501 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     4561 ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct
     4621 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     4681 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     4741 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     4801 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     4861 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     4921 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     4981 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     5041 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     5101 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
     5161 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     5221 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     5281 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     5341 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     5401 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     5461 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     5521 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     5581 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     5641 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     5701 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     5761 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     5821 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     5881 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     5941 ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag
     6001 cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc
     6061 cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc
     6121 tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg
     6181 agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc
     6241 aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg
     6301 atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg
     6361 gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc
     6421 agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg
     6481 ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta
     6541 ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac
     6601 caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac
     6661 aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga
     6721 agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag
     6781 taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc
     6841 atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag
     6901 tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg
     6961 gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac
     7021 cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag
     7081 ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc
     7141 tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt
     7201 ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg
     7261 gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac
     7321 cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg
     7381 ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa
     7441 cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg
     7501 ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc
     7561 gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa
     7621 gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata
     7681 atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag
     7741 gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt
     7801 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
     7861 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
     7921 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
     7981 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
     8041 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
     8101 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
     8161 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
     8221 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
     8281 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
     8341 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
     8401 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
     8461 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
     8521 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
     8581 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
     8641 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
     8701 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
     8761 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
     8821 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
     8881 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
     8941 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
     9001 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
     9061 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
     9121 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
     9181 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
     9241 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
     9301 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
     9361 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
     9421 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
     9481 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
     9541 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
     9601 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
     9661 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
     9721 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
     9781 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
     9841 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
     9901 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
     9961 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
    10021 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
    10081 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
    10141 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
    10201 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
    10261 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
    10321 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
    10381 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
    10441 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcct
//
LOCUS       DAZKAS010000093        10353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000093 DAZKAS010000000
VERSION     DAZKAS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.095"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8H7_001977"
     CDS             6..305
                     /locus_tag="ACG8H7_001977"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8984054.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8H7_001978"
     CDS             378..1739
                     /locus_tag="ACG8H7_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP8984055.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8H7_001979"
     CDS             1761..2021
                     /locus_tag="ACG8H7_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984056.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8H7_001980"
     CDS             2035..2787
                     /locus_tag="ACG8H7_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFP8984057.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8H7_001981"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8H7_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8984058.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8H7_001982"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8H7_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFP8984059.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8H7_001983"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8H7_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8984060.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8H7_001984"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8H7_001984"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFP8984061.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8H7_001985"
     CDS             7161..8558
                     /locus_tag="ACG8H7_001985"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFP8984062.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8H7_001986"
     CDS             8639..9079
                     /locus_tag="ACG8H7_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP8984063.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8H7_001987"
     CDS             9227..9553
                     /locus_tag="ACG8H7_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8984064.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8H7_001988"
     CDS             9554..10303
                     /locus_tag="ACG8H7_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFP8984065.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3285 a         1824 c         2146 g         3098 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aag
//
LOCUS       DAZKAS010000094        10325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000094 DAZKAS010000000
VERSION     DAZKAS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.096"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..1523
                     /gene="arcD"
                     /locus_tag="ACG8H7_001989"
     CDS             102..1523
                     /gene="arcD"
                     /locus_tag="ACG8H7_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFP8984066.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1537..2655
                     /locus_tag="ACG8H7_001990"
     CDS             1537..2655
                     /locus_tag="ACG8H7_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP8984067.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2715..4007)
                     /locus_tag="ACG8H7_001991"
     CDS             complement(2715..4007)
                     /locus_tag="ACG8H7_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFP8984068.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            4157..4717
                     /locus_tag="ACG8H7_001992"
     CDS             4157..4717
                     /locus_tag="ACG8H7_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8984069.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            4846..5223
                     /locus_tag="ACG8H7_001993"
     CDS             4846..5223
                     /locus_tag="ACG8H7_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFP8984070.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5156..7228
                     /locus_tag="ACG8H7_001994"
     CDS             5156..7228
                     /locus_tag="ACG8H7_001994"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP8984071.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(7439..8386)
                     /locus_tag="ACG8H7_001995"
     CDS             complement(7439..8386)
                     /locus_tag="ACG8H7_001995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8984072.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            8632..9096
                     /locus_tag="ACG8H7_001996"
     CDS             8632..9096
                     /locus_tag="ACG8H7_001996"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP8984073.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(9158..10201)
                     /locus_tag="ACG8H7_001997"
     CDS             complement(9158..10201)
                     /locus_tag="ACG8H7_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFP8984074.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         3438 a         1933 c         2151 g         2803 t
ORIGIN      
        1 aatatgaata aagttgcttt ttgttaagca agagcagttg caaaatagaa gggaatcttt
       61 tgcaagtgat ttgttcaatt ttataagtga gggtgatgga aatggaaaag aaatcgacgg
      121 gtattagcaa atggggactg gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt
      181 ttgggatcac aagtgatctg gctagttctg cagctccagg gccagcgatc ctttcatgga
      241 taatcgttgg gattggtatt ttagctttgg tattatcgct gaatcattta ggagaaaagc
      301 gtccagatct agatggtggt attttcggtt acgcggaagc ttcatttggt aaattaggtg
      361 gcttcatcag tggttggggt tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa
      421 cgatgctgat gagcgccatg ggagaatttt tcccgatatt caaaggtgga caaaatgttc
      481 cttctatttt atttgcaagt atttttattt ggggtctgac tttattagta aacaatggag
      541 ttgaaggagc gagcgtcatc aatacaatcg tgacgatctg taaattagtg ccattattcc
      601 tatttttaat atttgtattt atcgcattta aagtgaatat attcactgct gatttttggg
      661 gtaacgtatc tgataatcta gtgaatggaa cagggcagca aggaacgatc tggttgcaga
      721 ttaaaggttg tttgatggtt atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt
      781 tagccaaccg cgcgaagaaa cgttcagaag cccagcaagc atcgatcatt ggtctgcttt
      841 gtttattgtt tatctatatc cttgcttcga tcttgccata cggcgtctta tctcaagaag
      901 agttagcaac tatttcacaa cctgctatgg caaatatttt aaaaggaatc gtgggaaatt
      961 ggggagcagc gctgatcaat atcggcttga ttatttcgat cattggttct tggttgtctt
     1021 ggacgatgct cccagcagaa acaacgatgt tgatggctcg agacggtctg ttgccgaaag
     1081 gttggggaaa aacgaataaa aaaaatgccc caacttattc gttagttgtt actgcaatct
     1141 tgactaatct attcttattg actttcttag ttactgatta tgcctatcaa tttgcctatt
     1201 ctttatgtac agcggcaatt ttgatttgtt atctacttgt cgggatctat caagtccagt
     1261 tttctttaaa agaaggaatc aaaaaacaag tagtgatcgg tgggattgct gtagtttttg
     1321 aattgatggg catcatttta gcaggatttt cttatgtcct tttatgtagt attgcctatc
     1381 ttcctggttt ttgcttctac tggatcgctt gtcgtgaaac caaacatcaa attacgggaa
     1441 aagaaaaagt aatgattggg ctgatttgtg tggccgcact gctttcaatc gggttgcttg
     1501 ctgtaggctg gatcaagata taagggggaa agaaacatga aaatcgcaaa tttcggtgta
     1561 gaagaatggc taaataaatg ggaaaagaaa gcggtatatg atattgcaca aagctcgatc
     1621 gaagcattga cattagacga actcgttggc ttagatggga ctagcgtaag taaatttttc
     1681 gaaaaacgca aaaacgaacc attggactat ggctggatcg aaggatcaga tgttttcaaa
     1741 caagaagtcg cttctcttta tcaaaccgtc gatccggaaa acatcttaca aacgaatgga
     1801 gcgactggtg ctaatctttt agctctatac gcgttagtgg aagcaggaga ccatgttgtt
     1861 tcgatgcttc ctacttatca gcaattatat gacatcccca aatctttagg tgccgctgtt
     1921 gactttgttc atttgaagga agaagaagat tggcagtttg atttagaaca gctagaagcc
     1981 ttagtcaaac ctgaaacaaa aatcatctgt ttgaatagtg caaacaatcc tacaggcaca
     2041 ttgctagatc ggaaaacttt agaaaaaatc gcagaaattg ctcgtacagt agatgcatat
     2101 gtattgatag atgaagtata tgcgccttta acggataaag gtgaattctt gtcgatcgta
     2161 gatgtctatg aaaaaggtat agcaaccaat tccctatcca aaacctactc cattccaggt
     2221 attcgtattg gctggacagc tactggacct gagttggcag aagtttttcg aaagtatcga
     2281 gactacacga tgatttgcgg cggagtcctg tcagatgatc ttgctgtaca tgcattgaag
     2341 aataaagaaa aaatattggc gcgaaacaaa aagatcatta cagagaacct tgtgatttta
     2401 aaacaatggg tagccaatga accgaaagtt gaacttgtcg ctcccaatta cgtctctact
     2461 tctttcatta aattagccat cgaagaagat gaccagacat tttgtatcaa tttattagaa
     2521 gaaacaggag tactattagt accaggaagt gcctttgatt taccgaagca tgcccgacta
     2581 ggttactgtt gtaaaaaaga aaccttggaa aagggattat tcctcttatc tgagttcttg
     2641 aaaaaacagg cctaaacaaa aacactgcaa ggacagtctg tgacaactgt tcaagcagtg
     2701 tttttcattt acgcttattg tctatgagaa ggagagtgcc ctgaagctat ggagactcct
     2761 gaagcttttt cttctgcagt ttcttgctga tggatctctt gtgtattgtg tcgtacaagc
     2821 aagaagttga ccaaaacaag aagagaagtc gaaagaaaga cgccacgata accaaatgca
     2881 ctggaaacac tggagccgat cataggccct actacgttac cagttgcttg gaaagattgg
     2941 ttataactga agatccgtcc agcagcttga tgaggcgaat attttgtgat caatgcttgg
     3001 acagcaggga gcagacaagc atcagagata ccaatcaaaa aacgtaaggc agctaattgc
     3061 caaacattcg tgacgaatgc cattgggata taaaccagaa tcgcaaaacc caaaccaatc
     3121 gcaagtattc tttcactccc aatcttgtca cctaagcgac caaatcttgg agcggctatc
     3181 aaagtggcaa tcccaggtat cgaagcaatg attccgctga tcaacgtaac attcccatgt
     3241 ccatgcagca attggcgaat gtacagactg atgattggac taatggaatt gttggatgct
     3301 tggatgatca tcgttgtgac gaacataccg atgacgacgt gtggatattt caagtctttg
     3361 aaaatctgtt tggcagaaac catgttttct tttttcactg ggacaaattt ttcatggacg
     3421 aaaaacagac tcaacaagaa gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg
     3481 cgatagccaa acgctgaagc agagatcccg ccaagaagcg gtccaagtaa tgttcctgta
     3541 acagaaccag ttgctaaggt tccaagaact tgaccacttt tttcttttgg cgtcccagtt
     3601 gcgacaaggg cagtggcatt actgatataa ccagaaaaaa tcccttgaag cagacgcaat
     3661 gcgactaatt gatacacact agtcacgcaa cccatcaaag aaatgacgat tgccattcct
     3721 aatgaagcac gcaacagcat caattttctt cctttttggt cggctaattt tccccaccaa
     3781 ggagagataa gcgtagtcac caagaaagta gatgaaaaag tcaatccgct ccaaaagttc
     3841 aattgtgacg tactataatt acctaacgta tctatgtaaa gtgacataaa aggcatgact
     3901 aaactaaatc cgataccagc cataaatgtt ccaaaccata agacaaacaa gttttgttcc
     3961 caagtttctc ttttcctaaa taatttttcc tttattcttg cagccaatta gactccttct
     4021 ttctgaaaat tttcatctac agctctttat tataaactac aatatgtagt ttatcaatga
     4081 aagtctcttt tttctctttc tttttcgtgg taaactgaaa gagtgaagga tttattttat
     4141 gagaggaaac atggaaatga aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt
     4201 tcgagaacta gcagaagaga tggatgttca gcaaataacc tttcagcatt tagccaaacg
     4261 cttggatatc aaggctccct cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac
     4321 agcgctaaca gcgaaacttc tgcaggaact taatgaccag ctgcgacggg ctcttgtggg
     4381 aaaaagtggt caggaagcac tgcagatcta tgcagaaact tatcgatcct ttgctttttc
     4441 aaatcaagca gtgtatgagt tactgatcag tgtaccgcat accaatgaag ctgttttatt
     4501 agatggaatc catgaaacga atcagatcat tttgcagata tttgaggctt ttcaattaac
     4561 gaaagaagaa aaagttcatc gaagcaggga acttcgtagt atgatccacg gttatctttc
     4621 cttacgtttt ttaggatact ttacaaaaga accaacaatc gatccagaag acagctattt
     4681 ttggatgatc aataacttta tcgctacttt gccttaaaca atcagattca tttgtaacaa
     4741 ttttaagatg ttcattaaat gaccctttta taagagaaaa ttcttgcaac tttcttgaaa
     4801 agtgactaag atgagtctag aaaagctgaa agaaggagca cgagtatgta tcaagtaatt
     4861 accatgttcg gggacaatga gccttggtgg ttttttgaag actgggaaga agatattgaa
     4921 gaagaagaaa cattcgagtc tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac
     4981 gaaatccatc aaaattatga atatatcaat gcaaaaagta actttctcag cgccttttgg
     5041 aatgacggag atgagcgctg gtgtgaagag tgtgacgatg atttacagca atacaaggga
     5101 cttgcgttgt tgaaaaatca tcagcccatc acagtagaaa gcagaaagga attttatgaa
     5161 acaactaatt ctagcggaaa aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc
     5221 taatcaaaag cacaaaaatt attatgaagg acccaaggtg atcgttactt gggcccttgg
     5281 tcatttatta ggattgaaaa tgccggaaga tctgaataag gaatggcaga gctggcaaat
     5341 ggacacgttg ccaatgatcc ctaaaaaact aggaatcaaa ccacttccta aaacaggaca
     5401 tcaattaaaa gcaatcaagc agctggctca gcgaaaagat gtctcagaag cagtcattgc
     5461 gacagatgcc ggaagagaag gggaacttgt tgctcgctgg atcttagagt atgtgcattt
     5521 caataaacca gtgaagcggt tatggatctc ttctcaaaca gataaagcaa tcaaaactgg
     5581 attcaagcaa ctaagacctg cgaaagaata tgatgcattg tatgaatctg ctcttgcccg
     5641 ggcaaaagcg gactggctgg taggattgaa tgtgaccaga gccttaactg tgaagtacgc
     5701 ggataattta tcagcaggcc gtgtgcagac accgacacta gcattagtga gagaccaaga
     5761 aaagaaaatc gagcagttcc gtccacaaac gtatttcacg atcacgttgc aagctggaaa
     5821 agaacaagcc aaaatgcttc aaaaaaatca atttgcttta aaaacacaag aagaagcaca
     5881 aaaacttgtt caattgatca gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt
     5941 aaaaacggaa tctgcgccat tgccttatga tttaacagag atccaaagag aagccaatca
     6001 acgctatggc tattctgcaa agaaaacatt aagtcttgta caaagtttgt atgagaccca
     6061 taagatcgtt acttatccgc gtacagacag caaatatctg acaaatgaca tgaaaagtac
     6121 gatgaaagaa cgattgcaag cagtcagcga ctttgctcct gaggtcaagc agtatttgaa
     6181 aaacggagcc atcgtccaac agacaaaagt attccaagac agtaaagtga ccgatcacca
     6241 tgcacttttg ccaactgaaa acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa
     6301 aatctatcaa atgattgtct ctcgtttttt aggactgttt gctcaacccc ataaagtcag
     6361 ccaaacaaaa gttacggttg aatttggtaa agaacgattc atttttcggc aaaatcgtgt
     6421 cgtccaagca ggatggaaag gcgaaactga aactgagacg gaaacagtca aatgggaaaa
     6481 aggaatgaga atcaatcccg atttcgccat caaaaaagaa ctatctgcac cgcctaaacc
     6541 attgtcagaa gcaagcttgc ttggtcaaat ggaaaaatac agtctgggaa ccccagctac
     6601 gcgtgcagag attatcgaga aactaatcaa atctgaactg atggaacgta cccctcaagg
     6661 actgcaagta tctcctaaag gtaagcaatt attagtattg gtaaatccct ctttagtaac
     6721 accagatttg acagaaagct gggaaaaaga ccttgaagct attgctgcag gaaagaaaca
     6781 agcaggaacc ttcttgaatg gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa
     6841 atcaagcaaa caagaatatc aagatttctc gattacccaa aagaaatgcc cagaatgtgg
     6901 ggccaatctg cgcgagaaaa acacacgtga cggcaaaatc tatgtctgca ccaattcaga
     6961 ttgcaactac cgcagaagaa aagatccaaa agtctctaat catcgatgcc cgcaatgtca
     7021 ccggaaaatg gaaatcatcg aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg
     7081 aacgacagaa aaattgcttg ataaaaaaga gcagaagaag aaaatgacca aacatgaaga
     7141 acgacgcttg ctgaaaaaat atgcacaagc agaagaacca gaagaaagcc cattagcagc
     7201 agcactaaaa gcagcaatga agaagtaata agatatgaaa gaggttgtga tagaagtggt
     7261 cagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc
     7321 ggcttatttc cgaagaagct acttctggaa caccatttat tcggaaaaaa ggactgtgac
     7381 aagacttttg tcacagtcct ttttcattta aaaaaatgtt tacttcattt gacgtatatt
     7441 attggtgagg cttcaattca tttggcttca ctttttctgt ttctcttatg atataaacag
     7501 gacgctgttt cgtttctaag aagattttcc caatatattt cccgatgatt ccaagacata
     7561 acaattggat tccaccaatg aacaggaaga tcgagaccat ggaaggccat cctgatgtag
     7621 gatcaccaaa caacaatgcc cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac
     7681 aagacaaagc gccgataaat gaagcaatcg ttaacggtgc atccgagaag ttcacgatac
     7741 catcaatcga gtagttgaat aatttccaga aggaccaaga tgtttcgcca gccacacgtt
     7801 cgcggttttc ataagaaata tattctgttt taaatccgac ccagctaaat aatccttttg
     7861 aaaatcggtt gtattctgtt aattctagga cggcatccac catttgacga gtcatcaagc
     7921 ggaaatcacg agcgccatcg accatttctg tatcgctgat tttattgatc aactggtaga
     7981 attttttcgc aaagaaactg cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc
     8041 cgacacaatc gatatcagaa gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg
     8101 gatcttgcag atcaacatcc atcacggtca ctaaatctcc tgtagaagct tgcaatccag
     8161 cgtacagagc cgcttctttc ccaaaatttc tggaaaatga gacaaagcgg acataaggat
     8221 aagctgaggc tagcttcttg attgctgcta acgtaccgtc ttttgatccg tcgtcaataa
     8281 acaaatattc gaactcatgg gcacctttga atttttcaac ctcatcaaaa aaaagaggaa
     8341 cagttttttc ttcattaaaa catgggacaa caattgatac tttcatcgtt tctatccttt
     8401 caatcgtatg tttatatatg gtttacattc aaaatttaat aaaccttaaa taacaccttt
     8461 aaaatttgat tttacgtaaa agaacaggta ttctttttgt aaactaacgt atttagttta
     8521 ccatgtttcc tcggtttatc aaacttttaa gaacattttt tattagttgg caagtagttt
     8581 tggatagaga aaaaggagga agttccagtc tattgactag gagcaaacaa aatgaaaata
     8641 cacacaccta tcggcttttt atggttagat gcagatgaat ccggattgac tgctgtatct
     8701 tttcagccaa tttcagaaag cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa
     8761 cagtttgaag aatattttgc gggaaaaaga aaagaatttg atgttccttt atctataaag
     8821 agaggtacgc cttttcagca aaatgtctgg aaagctttgt cgaccattcc ttatggagaa
     8881 atccgaacgt ataaagaagt agccgttgct gttgatagtc cgaaagctgt gcgagcgatt
     8941 gggcaggcaa atcgaaccaa tccattgcca atcgttattc cttgtcatcg agtgatcggg
     9001 caaaatggaa aactgacagg atatatggga aacgcaatag atggggttga aatcaaacgc
     9061 caattgttgt tgttagaagg atatctgcaa aattaaaaac agttgtgatt cggacattgt
     9121 tccatgcgag aatcacaact gttttttcat gttcattcta aactttgatc agccaaataa
     9181 atagtaaagc catcactgtt tggagaagcc cgacagctaa tccataagat aagaagcgtg
     9241 taccttcttt catgaatttt gcaaagtcta aacgcagacc aattgccgct aaagcagtga
     9301 tctcaaacca gctgctgata aaatgtgccg attggtcaat aactacaggt aaagaaaaca
     9361 agctgttgac caaacaaaag atcacaaacc ctaatacata ccaaggaagc ggtaattttc
     9421 gttttttctg cgtatcagtt ggcgtttcaa acagagctgg ttgtttcttg tgcatgaatt
     9481 tttcaaacag atagacaaca acaacgagca tcataatacg tagaatctta aatagcatgg
     9541 cgaattcgac aacagaattg tcaagtaagc ttgccccagc gactacttga ccaacagatt
     9601 ggagtgtacc acccaaaagt gcactttttt caatgagatt tccaccaaac aactgtaatc
     9661 caagaaaagg gagagtcaac atcatcacag tacccagtag attgactagt gtgatgatct
     9721 gtcctttttc ttcctctttc gcatggatgg aaggggcgat tgaagcaata gcagaagaac
     9781 cacaaacagc gtttcctcca gccatcatca aagaagaagt atcgctgaat tgcagttttt
     9841 taccaatcaa gtaagcaccg atgatagtga ctgacatttg cagtaacaca aaaacaattc
     9901 cttttacacc aatttgagca atcgtttgag ctgtgacagt tgcacctagt aaaaaaacag
     9961 agaattctaa tagtttgctt tcagcaaatt ttgtgccgat tgctagaaca ggctgtttaa
    10021 aaaaagtatt tcctaataaa atacccaata aaatagcaat agtcgctcca cctaactgtg
    10081 gcagataaat agcaagaagc ctgctgaagg cagcgatggc aaaagaaagc agtaaaccgg
    10141 gtaagatttc gcggaccgct gaatccttta cagattttat tggatgtgac aatgatttca
    10201 tttgatcggt tcctttcgtc aataaatact aaaagcatac tcgaacatag tagcactaaa
    10261 ccagtatttt gacaaaagaa caagaggtcg tgacaaaagt cttgtacgac ctcttgtttc
    10321 gaata
//
LOCUS       DAZKAS010000095        10209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000095 DAZKAS010000000
VERSION     DAZKAS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.097"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..276
                     /locus_tag="ACG8H7_001998"
     CDS             127..276
                     /locus_tag="ACG8H7_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFP8984075.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            287..1801
                     /gene="dltA"
                     /locus_tag="ACG8H7_001999"
     CDS             287..1801
                     /gene="dltA"
                     /locus_tag="ACG8H7_001999"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFP8984076.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1798..3003
                     /gene="dltB"
                     /locus_tag="ACG8H7_002000"
     CDS             1798..3003
                     /gene="dltB"
                     /locus_tag="ACG8H7_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFP8984077.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3048..3281
                     /gene="dltC"
                     /locus_tag="ACG8H7_002001"
     CDS             3048..3281
                     /gene="dltC"
                     /locus_tag="ACG8H7_002001"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFP8984078.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3284..4552
                     /gene="dltD"
                     /locus_tag="ACG8H7_002002"
     CDS             3284..4552
                     /gene="dltD"
                     /locus_tag="ACG8H7_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFP8984079.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4693..5775
                     /locus_tag="ACG8H7_002003"
     CDS             4693..5775
                     /locus_tag="ACG8H7_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFP8984080.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5775..6578
                     /locus_tag="ACG8H7_002004"
     CDS             5775..6578
                     /locus_tag="ACG8H7_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFP8984081.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6648..7388
                     /locus_tag="ACG8H7_002005"
     CDS             6648..7388
                     /locus_tag="ACG8H7_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8984082.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7399..8403
                     /locus_tag="ACG8H7_002006"
     CDS             7399..8403
                     /locus_tag="ACG8H7_002006"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFP8984083.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8448..>10209)
                     /locus_tag="ACG8H7_002007"
     CDS             complement(8448..>10209)
                     /locus_tag="ACG8H7_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984084.1"
                     /translation="LFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSK
                     YTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESN
                     PGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVL
                     TMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPF
                     VHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELP
                     ELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIY
                     QTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHI
                     QEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLIL
                     ARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIY
                     LSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQE
                     KDTFIDRITVQLSRNYPNPV"
BASE COUNT         3272 a         1695 c         2072 g         3170 t
ORIGIN      
        1 tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct
       61 agacacatgt ttctttttgt caaaattagt tacactttgt gtgaggttat atgaggaggg
      121 aatccaatga acaaatggcg ttctaatcca aaattgaatt attggggaaa attcatcatt
      181 cgaacaattt tttatagtgc agtactgctg ttcctgattt atctgtatca ctataaaaat
      241 attcaaggag gaacttttat ctataacgaa ttttaggagg aagagtatgg aaatcaaaac
      301 gattattgaa gcaatcgatg attggggaat aaatgaacct gatcgagttg cttatcaggc
      361 ggatgagacg catacttttg gtgaattgaa acaagcatca gacgcacttg cttattattt
      421 agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg agtttgaaat
      481 gattgtctca tttctagggg ttgtcaaatc tggccatgcc tatttgccga ttgaagagca
      541 tacacccaag gagcgtatcc tatcgatctt tcgggtggct aaaccatcta tggtcatcag
      601 tatcggggac tggatagaag atattccttc ggttcctgtc ataacaaaaa aagaatttca
      661 agaaatcaag aagataccag taggatttta tcccagaaat agcgtaaaag gtacagaaaa
      721 tttttatatt atttttactt ctggtactac tggagaacca aaaggcgtac aaatcagtca
      781 tgataatcta gtcagctttg ttcaatggac gatggaagac tttgggatca agcctggtat
      841 gcatttctta gcccaagcgc cattttcttt tgatttatcg gtattttcta tttatcctgc
      901 attagtttct ggcggaatgc tgaagccgtt aaataaagca gttgttcaag actttcgtca
      961 attattcgcg acattgccag ccttgaagtt gaatgtgtgg gtttctactc catcatttat
     1021 ggatatttgc ttgatggaac ctacattcaa tggagaaaat gtgccggaat tggacatgtt
     1081 tttattttgc ggagaagaat tgacgaaaaa aactgcagaa agtctacttg atcgattccc
     1141 aaatgcacgg atatataata cttacggtcc aacagaagca actgtagcta tttcaagtat
     1201 tcagattgac cagcatgtac tagatagtta tgaccgacta ccgattggct acgtaaaaaa
     1261 agacacgaaa gtttctattg tccaagaagg taaacctgtt tcaaaaggag aaacgggtga
     1321 aatcattatt gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg aaaagacagc
     1381 tgctgcgttc ttttgtcaaa atgacttagc aagttaccat acaggggatg caggtagact
     1441 agatgaatat gggcttttat tttatgaagg acgtatggat cagcaggtca aattacatgg
     1501 ataccggatt gaactcgggg atatcgaaca ttatctgctc caagacaacc gaatcaaaca
     1561 agctgtcgtc gtaccaaaat accaaggaac aaaagtacag cagctggttg cttttgtcgt
     1621 attagaaaac aaaacgaaac agccggattt tcagttgact aaatctatca aagaacaatt
     1681 actagaagtt gtaatggatt atatgattcc tcagaagttc cattatgtcg atctgctgcc
     1741 tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg
     1801 atgttacctc atatgatccc ttatgcagag ccgttttatt tttttctttt agccattgcg
     1861 cttgtaccga tcgttctatc tttattgata tgggaaaaaa gattaccagt ttatcaatca
     1921 gcagtgacac ttttcttttt atttgtcagc tttggaggta attattggca acagggagcg
     1981 gcgctgattg ggtatgtttt gtggcagacg atacttgtat ggggctattt tgtttatcgg
     2041 caaaggaaaa atcagacagg atggtttttt gccgccgtta ttttagcgat aattccactg
     2101 tttttaacga aagtgactcc tttttttgaa catggaaaag catcgttact tggtttttta
     2161 ggaatctctt atttaacctt taaatctgta caaatgatca tggaaataag agatggcatg
     2221 atcaaaacat ataagccatt tcgttatatc cagtttctgc tgttctttcc aacaatctct
     2281 tctggaccaa tcgatcgcta taggagattt gaaaaagatc agctccatcc gcctgttcgt
     2341 gataaatatg ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg ttttttatat
     2401 aaattcatta tcggttatta ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc
     2461 ttacagcatg gtggtatttc atgggcatta gtaggttata tgtacgtgta cagtatgtat
     2521 ttgttttttg attttgctgg ctatagctta tttgcagtcg gtacaagcta catgatggga
     2581 tatcagacac caatcaattt caataaacct tttttatcat ggaatatcaa agaattctgg
     2641 aatcgttggc atatgacgct gtccttttgg ttccgtgatt atgtatatat gcgattgatg
     2701 ttcttcatga tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa tatcggttat
     2761 tttgctttgt ttcttctaat gggtatatgg catggattga cgtggtacta catcgtatat
     2821 ggactgtatc atgcttgtct gatctgtctg acagatgcat ggatccgatt taagaagaag
     2881 cataaagagc agttgccttc taacaaattt acgcatggct tagccgtatt tttgacattt
     2941 caagcagtat gttttagctt cctgattttt tcaggatttt tagataaact tttttttagc
     3001 taacgaatag tgacatatta atttatataa caaaggagaa aaggaaaatg gaagaacaaa
     3061 ttttatcaat cttagaagaa ataacaggaa cagatgaagt aagaaaaaat cgtgatattg
     3121 atctatttga tgaagggttg cttgattctt tagcatctgt tcaattatta gtcgagctag
     3181 acggagagct ggatatccaa gtccctgtat ctgaattcga gcgagaggat tggagcacac
     3241 cgaataaaat cattcaacaa gcaactgcat tgaagggttg agtatgaaaa agaaactttt
     3301 tgctattttc ggcccggttg ttttagctgt tggcttactg tttgtgtttt ttacctcctc
     3361 ctatcaagta gatatctcta atccagaaac gattagacgt gcctcttctt ccatgtcagt
     3421 gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac aatatattcc
     3481 tttttttggc tcaagtgaat taagcagaat cagtccattt catccgtctg ttctggcaga
     3541 aaaataccag cgaaactatc gtccattttt gctgggagca cccgggacac aatcgttaag
     3601 ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat
     3661 tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact gattacttta atacgtatta
     3721 ttctgaactt caaacatacg actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg
     3781 ctatttagct agaagattat taactttctc aaaagtaaaa gagaatgata cgttgacggc
     3841 gattctgcaa acgatcaaaa aagggaagct acctttacct gaatctttga atcagctgcg
     3901 cagccaatgg aacatgctga agagagagga tgaagtattc agcaatattg ggttgaaaga
     3961 tcgacagcaa aaaatcgatc atgagtcaaa acgattacca aaacaatacc aagaaacaga
     4021 attatccatt ttagcaaatc aaattggaga acgagaaacg acgaataatc cattcgggtt
     4081 gaaaaacgat ttttatacgc atcgtatccg agcccacgag ccagagctca aacagtcaca
     4141 aaaaaattgg gattatcgtt tttcgccaga attttctgat tttcagctgg tcttggatca
     4201 attagcaaaa aaccataacg aagtattgtt catcatccca ccggttaacg agaaatggag
     4261 cgattatacc ggactatctc aagaaatgtt acaaggattt gccaaaaaga tcaaatttca
     4321 attaaatagc caaggattca atcgaatagc tgattttgtc aatcaagcgg gtaccaacta
     4381 ttttatggaa gacacgatcc atctaggctg gaaaggctgg ttggctgcag atcaacagat
     4441 cagaccgttt ttagaagaaa atcatattac tgctagtaag tatcatcttg acgatgcttt
     4501 ttttagcaaa tcttggcaac atcaaatacc agataaacta caattaaaat aaagaaaaca
     4561 ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt ctgaataaaa gataaaaata
     4621 ccaagtttgc ctgaacaaaa atcataagca atggtataat cacaatatga atatagaaat
     4681 gggggaaaac atttggatac aaaagaagaa cagctactta ttgatttaac ttctgcaaaa
     4741 ggagtacctg gaaatgaaga agaggtaaga gaagtattca gagaatatgc caaaccattc
     4801 gctgatgata ttttttatga cggtttaggc agtgtgattg ccaaacatgg tgctggcgga
     4861 ggccccaagg tctttatttc tggacatatg gacgaagttg gttttatggt aacaaaaata
     4921 accgaaaaag gattcttgga attccaaact cttggaggtt ggtgggggca agtcatgctt
     4981 gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt gattggttcc
     5041 aaaccaccac atgttttaac acctcaagta agaaataaac cttatgaaat aaaggatatg
     5101 ttcattgata ttggcgcatc aagccaagaa gaagccaaag agtggggcat acgtcctgga
     5161 gatatggtca ctccatatat tgaatacaaa cgaatgaatg gcagtaaata tttactggca
     5221 aaagcatggg ataatcgtat cgggacagct gtttctctgc gagtattaga gaatctatct
     5281 aaagaagcac acccaaatgt tttattcgca ggaagcgatg tgcaagaaga agtaggcttg
     5341 cgaggtgctc gaacaagtac acaccttgta aatcccgata ttgcttttgc tttagatact
     5401 ggtacagcag gagacactcc aggaatgaca ccaaaagaag cagattcgat tttaggaaaa
     5461 ggcccacaga ttttgatttt tgatgcatct atgatcccgc ataaaaaatt attgaatttt
     5521 gttattgatg tagcagagga aatggatatt ccattccaat atacagtgat ttctggcgga
     5581 ggaactgatg caggacaaat gcatttgaca agagatggtg ttccgtctgt agccattacc
     5641 gttcctgttc gttatctgca ttcccataca tcagtgattc atgaagatga ttatttgaat
     5701 acagtgaaac tagtcacaga attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt
     5761 cggagttatg aataatgaat aatagaatac ctatttctga atatcggaaa aaagcacgag
     5821 tttctttgga aaatcaatgg ggaatcaata cttggcttgt tttcttatct ataatattta
     5881 cgagcgtcat ccagaacttt tttgacggtt tcagtccttt tccggaagaa agcatgcagc
     5941 agcacttatt gagttttcta gtcgataatt tacttttatt cgcattcaac tatgcattgt
     6001 tttacattgc gctagtagtt gtacggggtg gcagaccaaa aagtaacctt ttattttcag
     6061 ttttccaaag aaagtatttt ggttcgatac ttggcatcaa tttgatcaat tcagtcatca
     6121 actggctact caacagttta atctttctgc ccatgttttt aatcagcgga gtgagtactt
     6181 atatgcagct gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca gaagattttc
     6241 ttgatatcag ctttgtttta tcgatgatag ggatgatttt tgtttcagca ctcatcatga
     6301 gtattgttgg cggtttattc caatttgctg catggacaaa gttcgactat cctgatttgt
     6361 cggttattca atgtttgaaa tatggctggt ttttattgaa agatagaatt ggtacgtata
     6421 ttttgttgca gctgtctttt atcggctggt atttgctagg agcaatcgct ttgttcatcg
     6481 gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc agaattttat gaacaagcac
     6541 ggattgaaaa aggaggtcca gacgcctatt ttacttagca tgtaagcagt ttattcatca
     6601 aaaaaataac ttttgtggta ggataaatct ttgaggggga atgtacgatg aaattaactg
     6661 ttttaggatg cttaggagcg tatccttata aaaatcaagg gacaacaggt tatttgcttc
     6721 aatctgacgg gttcaatttg ttgatcgatg caggtagtgc tacgctgatc aaattacaag
     6781 aacaccttga tccacttgat ctagatgctg tgatccttag ccattatcat catgatcata
     6841 ttgctgacct tggtgtatta cagtattact ggcaacttca tcctaagaga aatccggaga
     6901 cattgctttc aatttatgga cacacaaaag attccattca ctttgaagaa ttgacgttgg
     6961 aaaatgtctc aaaaggacat ccttattttg aagctgaaga attaaaaatt ggtccttttc
     7021 tgattacttt tatggaaaca atccatccag tagtatgtta cgccatgcgt atcgtagaag
     7081 aaacaacggg gaaagtattc gtatttacag gtgattcagg ttatttggaa agtttcgctg
     7141 attttgcgaa agatgctgat ttgtttctag cagataccta tctttttgaa ggaaatgaac
     7201 atcatcatgc ccatttcact tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa
     7261 agaaacttgt acttacacac ctacctcagt ttggtgattt agaacaattg aaaaatgaag
     7321 cgcaaaaggc agcaggcaaa gggattgata tagaacttgc tgcagttgac aaagtgttta
     7381 acatttagga ggaagaacat gatttttgta ccaaatgaaa accacgaccc aagaatcaat
     7441 ttagcaattg aacaatattt gctgcaagaa atgcctttag atgaacctat tttgctcttt
     7501 tatatcaatg aaccttcaat catcatcggc agaaaccaaa atacgcttga agaaatcaat
     7561 cgagattatg tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg aggcggagct
     7621 gtctaccatg attttggaaa cctgaacttt agttttatca tgcctgatga tggggattca
     7681 ttccgtgact ttgcgaaagt aacacagcca atcatccaag cacttcatga attgggtgtt
     7741 gctggggcgg aattgaaagg ccgaaatgat ctagtcattg atggcatgaa attttctggc
     7801 aacgcgatgt atgctaccaa cggtcggatg tttgctcatg gaacaattat gttcgactct
     7861 gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga
     7921 atcaaatcga tccgttctcg tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa
     7981 aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga aaattttcgg cgtagagtca
     8041 gtagaagaag taaaaacgta cgaattgact gatgaagatt ggaaaaaaat caatcgaatc
     8101 tctgaggaat attatcgaaa ttgggattgg aattacggga agtctccaga tttcaatttc
     8161 tctcgccaaa aacgtttttc tatcggttcg attgaagtcc atttgaatgt ctcagaagga
     8221 atgatcaaag aagcaaaaat ctttggcgat ttcttcggac taggagatat ctctgacgtg
     8281 gaaaaaaaat tggttggaca gaaatatgat aaagcatctc tatcaaaaat agtcagccaa
     8341 attgatgtga agaaatattt tggtgcgata gaaccagaag aattgcttac cttactttat
     8401 tgaaaataaa aaatagcgga atccacatat atggattccg ctatttttta tacaggattc
     8461 gggtaatttc gagagagttg aacagtgatc cgatcaatga aagtatcttt ttcctgcgtt
     8521 agtgttcctg tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa acgttcctga
     8581 tcatctaatt cttctgtgat gcctcgacta aataggtttg tatcgtaatg tgcactatgt
     8641 tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat ttttcgatac
     8701 agtggatttt cgatggcatg agtttctcta aaggaatgct ctaaagcgtg gatcaaaagt
     8761 tgagccagat cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg gaaggttatc
     8821 tgaatattcc gtttggcagc taacttctgg taacgcgcta agataagatt tgtcacctgg
     8881 tggttgcaaa aagaacacag catttcgaaa ttttttgctt cttctaatat cttttccaca
     8941 tagtttttag cttttgtttg gtccgtatgc attaaatctt gcagattctc catctcttgg
     9001 acaaattcca ggcgaagtgt atttacttct tggatatgct gtcgaagctg ctcataatgt
     9061 tgctccaaca aatcattttt gatttttaag aaatctactt gtctccgatc tttgtcggta
     9121 tttaagatgt gatcgattag attgtagctg ataataacga ttagaatgat aatatataaa
     9181 atcgtagacc aattgatcgt tgtttgatag atacctaatg ccgtttggat gtaaatcaag
     9241 caatggataa tggctaccaa gacgatccaa ggtgtacaag ctacataaaa acggttattc
     9301 ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag tgcttataac actagcaata
     9361 ttagcaatcg gcattaattc aggaagttct gcaggagtaa aagttgcata aagcagaacg
     9421 ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt accatttttt atagtaaaac
     9481 attcggctcc aatagtagca gatgatgaaa gctgacgtta agataataaa taaatgagat
     9541 aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta aaatatcttc tgatacagct
     9601 tcaaatccta atgccaaagc aaagcaactt aagagaataa gtgagtattc aagacgtttt
     9661 tgtttataca tcaagtagat cgtatagaaa aaggtcccta tagcgataaa aacagcgatt
     9721 atcatcgtca atacttgcgg cactgattgt gaataaataa aactaatcaa tggtccagtt
     9781 gttcctagat aaactttcgg tggcagtcca gcatagttct gataaggaga tgagactgta
     9841 agggtcaatt tttttccgat ataatttgct ggtaaatcta tcacagccaa tgagagtccg
     9901 ggattgcttt cttcctgata atctttccgt tggtataaaa tcttttcgcc tagatataca
     9961 gtaatccatt gatggtttcc tttgattagt aaatttgctt cgtccaacgg tttttcaagt
    10021 gtcgtttcca taatgaatgt ttcgtcttta tccacggtag gaatcgtacg agtgtacttg
    10081 ctgtcaaact taaaatcagg atcatctttt gtatagaaaa cccacgaatc ttttatttct
    10141 ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa agaaataagc aattcctgtt
    10201 aaaaataac
//
LOCUS       DAZKAS010000096        10202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000096 DAZKAS010000000
VERSION     DAZKAS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.098"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..481
                     /locus_tag="ACG8H7_002008"
     CDS             8..481
                     /locus_tag="ACG8H7_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFP8984085.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            490..717
                     /locus_tag="ACG8H7_002009"
     CDS             490..717
                     /locus_tag="ACG8H7_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984086.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1353..1724
                     /locus_tag="ACG8H7_002010"
     CDS             1353..1724
                     /locus_tag="ACG8H7_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984087.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1980..2222)
                     /locus_tag="ACG8H7_002011"
     CDS             complement(1980..2222)
                     /locus_tag="ACG8H7_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP8984088.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2254..3156)
                     /locus_tag="ACG8H7_002012"
     CDS             complement(2254..3156)
                     /locus_tag="ACG8H7_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP8984089.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3169..3357)
                     /locus_tag="ACG8H7_002013"
     CDS             complement(3169..3357)
                     /locus_tag="ACG8H7_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP8984090.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3371..3934)
                     /gene="amaP"
                     /locus_tag="ACG8H7_002014"
     CDS             complement(3371..3934)
                     /gene="amaP"
                     /locus_tag="ACG8H7_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP8984091.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3972..4865)
                     /locus_tag="ACG8H7_002015"
     CDS             complement(3972..4865)
                     /locus_tag="ACG8H7_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8984092.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4943..5866)
                     /locus_tag="ACG8H7_002016"
     CDS             complement(4943..5866)
                     /locus_tag="ACG8H7_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFP8984093.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5915..6265)
                     /locus_tag="ACG8H7_002017"
     CDS             complement(5915..6265)
                     /locus_tag="ACG8H7_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP8984094.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6298..7200)
                     /locus_tag="ACG8H7_002018"
     CDS             complement(6298..7200)
                     /locus_tag="ACG8H7_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP8984095.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7193..8050)
                     /locus_tag="ACG8H7_002019"
     CDS             complement(7193..8050)
                     /locus_tag="ACG8H7_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP8984096.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8384..9193
                     /locus_tag="ACG8H7_002020"
     CDS             8384..9193
                     /locus_tag="ACG8H7_002020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8984097.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9233..9730
                     /locus_tag="ACG8H7_002021"
     CDS             9233..9730
                     /locus_tag="ACG8H7_002021"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFP8984098.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10115..10188)
                     /locus_tag="ACG8H7_002022"
     tRNA            complement(10115..10188)
                     /locus_tag="ACG8H7_002022"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10152..10154),aa:Ile,seq:cat)
BASE COUNT         2946 a         1937 c         1757 g         3562 t
ORIGIN      
        1 gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta taagtgccgt
       61 atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa aaggattcaa
      121 ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc tcctagggat
      181 cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc aagctttttc
      241 cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta ttggtttcgc
      301 cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg ctccaggttt
      361 gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat taacaagcgt
      421 gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt tgacagtgtg
      481 aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa aaaaatgagt
      541 gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat tctaaaaaat
      601 ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt tttggagtgt
      661 caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga agaataaaat
      721 aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga gacttttaga
      781 agttatttac gtctattatt cagatttttt atttcgattg aataccgttt tatctataaa
      841 taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact atcacttttc
      901 ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat aatactttat
      961 tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt attacaggcc
     1021 attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg tgttaactct
     1081 tactccattc ttttttagta agctcccata tatactgtaa agccttctat atattatgtc
     1141 atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata acagtgatgg
     1201 acaattcttt tttattttta acattatttt gataataatg attatttatt taaaaataca
     1261 ttagataaca tttgttacga cacgaaaaag cttcacaaac aaatatcgtt attttaaaat
     1321 gagggcgaac tcacacaatt aggaggaata gtatgccatt tattgtttac ttttttcttt
     1381 cattactcgt gatctatatt ccattaccaa catttttcct gctcgtcaat cgtctagcag
     1441 agcaacatga tacgattgct gctcttacga aactttgttt acttctattt gtttgtctag
     1501 attactttac gagctattat tggctaaaac agacaagagt aaaaaagaga tattatttct
     1561 tccttgtctt tttcaatctc tttgagtttc tgatccattg ctttttactt catcagtatc
     1621 atacgatcca tttgtggatc atctgggttt tcaaagtggt cgtattattt tgtatgttca
     1681 cctttcctat ctcaagaaaa tttagggagg cattgtttta ttaaagaagc ttctctgtat
     1741 caataaagac acatttaagg aaaaactttt aaaaatctaa ttgggaattt gcccgcataa
     1801 ataagaggct gtgacaaaag tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa
     1861 agcttgcatc ttcgataata tgcccaacca aatgaaaaat atgagaagct atttttgttt
     1921 ggctgttctt attacttgat gaagaacagc tttttcacga cctcttttta aaagtctttt
     1981 tacagttttc gagcaaagaa tgatacgatt agtactaaaa tgatggcacc aatcaatgat
     2041 ggcacgattg ccattcctgc aatacttggt ccccaagaac ctaataatgc ttgaccaagt
     2101 gctgatccaa ctaaacctgc tacgacattt gcaataatcc ccatcgaaga acctttattc
     2161 gtgattgctc ctgcaattgc tccgataatt cctccgacga taagtgacca aataaatccc
     2221 atgtttcgtt cctcctaatg ttttacttcc ttattactga acccgtggtt ctgtattttc
     2281 ttttactttt tgtgcgccgc ttccagctgc tgattttact ttatctgttt gattcgaagc
     2341 aaattcaccg gtttttttcg ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt
     2401 ttcaaattct tcttttgttt tgatgtccac tacgttagcg ttgacttcga tcacttctaa
     2461 atgtgtcata ttttttactt ctttgccgat tacttcttgc atttcttcaa agattttttc
     2521 gatatctttg ccatattcca caatgacatc catatcaact gctacttgtt ttttaccgac
     2581 ttctgtttcg atcccagccg ttacattatc tgtattcaca agttttcctg ccacattaga
     2641 gaagaaacca ccgtttgcag ataataaacc gtctacttgt tcaattgcga ttccgatgat
     2701 tttttgcaca actttatctt cataattcag ctcaccgcgc attgaatctt cctgttcaga
     2761 atcatgcttt ccttggttat ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg
     2821 gtcagaacta agtttttctt gtgctttcgc atggtcagaa ggatgttttt gctcttccac
     2881 aggtttagaa ggatcgattg catcatgttg ttctggaact ttagattctg gagttcctgg
     2941 atgatgaggt gcatgctttt ctgcatcatg tttcttctct tctactcctt gttgaacgtt
     3001 agggactact ggatctggat gtgcagatac tggtgctgtt ttgttctttt catcgttcac
     3061 accatttgat tggtttggtg tcgttgggtc tggatgtgca gacactggtt tttggttgtt
     3121 cattttattg ttattatttt tgttattgtt attcatggat gtttcctcct aagatttaaa
     3181 atagtgatct aagattttcg tttgtttcag ataaaaaccg atagcgacgc cgatcgctgc
     3241 catgatcaag atcaataagg tcttgaaaaa gcccacggtg atccataaaa cggctaatat
     3301 caatccaagt accccaaaga tgatagggag cttgtatgtc gaaaatagtt ctttcatttt
     3361 tggtcccccc ctattctact cgtttgcggt cattgcttcg tttagctggt tcttcaaagc
     3421 ctgtgtattt caccgatact ttcactggct cttttgttcc caatatgtgt tgtacttcat
     3481 tttcgatttc tttcatcaat gttcctgttt taccaatgag tgctgaagtt cgtttcaact
     3541 ccccttttac attgacggcg atcttgtttt tcgttgcttt gacatttact tttggtgaat
     3601 ggatgaattc atcttcatgc aagtgagaac gaaccattcc ttcaattgct tttttatcca
     3661 acgtcaactc cccatcacca tgtttcagta gaaagtttcc gattgttttt ggataaaaca
     3721 ggaaaacaag taacgtgata attgtcaaaa tagcaaatgc aacggcaagc caaaatacat
     3781 attgttgcac gtatgaatta gtgatggaaa agaatcgaaa cttctctagc tgaaaaggca
     3841 atggataggt tgcacttaat agtatcgcaa acaaaagcac agatagcaaa agtaatgttc
     3901 cgatcactcc gatcgttttt acacctttat tcattttcgc acctcctcat aggggtgaaa
     3961 cgatcaactt cttataagtt gatcgcttct ccaccagtta ctccgtaaat ttgggctgta
     4021 acataacttg ctttattcga tgctaagaaa acataaactg gtgctaattc tcctggctga
     4081 cctgcacgtt ctagtaaact ttgctgacca aattcaggga tcgaatcaga aggttgtcct
     4141 tcgtctagtt gtaatggtgt ccagattgga ccaggtgcta caccatttac acggattcct
     4201 ttaggtgcaa gctgttttgc taaaccgatc gtaaagttag cgattgaagc ttttgttgct
     4261 gcgtaatcta ataaatgttc gcttggattg aatgcttgaa cagaagttgt tgttacgatt
     4321 gctcctcctg ctgggatatg cggaacagct tctttcacta aagcaaacat agcgatgatg
     4381 tttatcataa atgtgtcttt gacttgctca agtggtaact cagcaataga ctgacaagaa
     4441 atttgctgtc ctgcgtttag taccatcgta tctagcccgc caagttcact cacggcttta
     4501 tctatgattt ctttaggtgc ttgttcgtca cgaagatcaa aaggtagtaa gacaatctta
     4561 cgtcctgcat ctttcacgta ttttgctact tcctctgcat ctttttcttc tcctgggaag
     4621 aattgaagag cgatatctgc cccttctcta gcaaatgcaa tcgctgctgc acgaccgatc
     4681 cctgaatctc caccagtgat tagtacacgg cggttttcta gttgattatt gccttcatag
     4741 gattcttctc cacaatcagg aacaggaatc atattctgct gtaaagctgg tgtatcctga
     4801 tcttgtttag gaaatttctc cgtatggtac aagtttcgcg ggtcgattaa ttttggttct
     4861 gtcattgctg tattcctcct agtttttaaa ttgtattgcc aaaacaaatt tctttgtttg
     4921 atacctgttt taaatagatt ctttaatttg tataatcttt ccggtataaa tggtaaccta
     4981 aaactactgc tgctatcaaa gagccaaaga ctacgagtat aaagcctaga gcagaacttt
     5041 tacccggtaa tcctcctgta ttcattcccc aaatccctgc gaacaacgcc ggaacggaaa
     5101 tgattaatgc agctgaatct agatatttca tgatatgatt tagattattg tccatcatcc
     5161 cgttgaatag atcaccaata ctctctaata aatctcgata gatctcgatc attttttctg
     5221 tttgtcgctg tctcaaatgg acatcataga gtagctgttc atcgtctaaa cgtttgatga
     5281 aagtttcaga attccataat tgatccattg tttcccgttg atctcttaag gtatgatcga
     5341 tatatacgat gtctctttca agatctgcct gtttatacag ttcttcattt tccgttgttt
     5401 tacgagctgc tttatccaaa gcgtcaattc gctttttcat ctctcttagt tctttcacgt
     5461 aatgggtgta aatatctaat aatgtatacg tgatgatctt ttcaaacgag tttaaatgat
     5521 ttccatgctt tgtcagcagc cgatccacaa attcggtttt tctatcgata caagtaatca
     5581 agagattatc ggaaagtaca aaagatattg gcgttaattt tttttcgata cgattatctg
     5641 actgatctag attaatcact accaaagaaa atggatgctc tagtttcgtc tcataaaggc
     5701 gctccatccg agaaacttct tccggatatt cggttcctac gaaaatatca tcaggaagct
     5761 cgaattcttc gattgcttgc tcaatctctt cgggctttgc actatcaagt acgagccaat
     5821 tatatttttt ttcatctgtt tctattacat tcttttcttc atccacaaag tagtatctca
     5881 atgtatacct cctttttgga ctttctcgaa actattagct gaagaagcag ttctttaatt
     5941 tgatattata atcgagtact gcgatttcat tttctaatga aaagctgtca agctgtttgt
     6001 tgaagtccat gttcaatcgt ttaccgaaat aattttgctc taatttagat gtatagatgt
     6061 ccaataatgg atgctcgatt ttagtcgcat attctccaaa ttccgaaaca aaaggaatga
     6121 tttgaaaaaa gctgcctttt gcaaaagcac ctttgtccct tagaaatgga tcggaatcat
     6181 tgataagcgc gagtgcgatt tttgtatttt ccccaacctt ttcaattaac gtctctactg
     6241 catttttaga cattgtaatt tccattccta ctccccctct tcagttgttg tcttctatta
     6301 tttaacaact acatctgttt ttggttgaac ttttgcatat tttggatatt tagggaattt
     6361 agctacacaa cggaaagccg tcatccctgc atatttttcg taatatttct tcgttggccc
     6421 ttcaaaatcc ttgctccacg gtttcacatg tcctgaaaaa tggataatgt aaggattgtt
     6481 gcgagtttct tcgtattcac gttcacctgc tgctgttgga tgtttttttg cttttgccat
     6541 gatatagcct tgtgcattcc aacgcggatg aagtggtaac caacggtcat gtaagatagc
     6601 atttaatgca tcttggtcat ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa
     6661 tactttttgc gtaatatttt catctaacca ttttttcaca ttgatcagca tcaaacctga
     6721 gttgaaataa cgcattgatt tagctgaaat tttcattttt tccagtcgtt gatggaaccc
     6781 agcgtcttca acagctgcta cgattgaatc accgaagtca agacgccata atttagagat
     6841 atcttgtaaa gcgatcatgt cgcaatccat atataagata cgttcaactt ctgtgccgcg
     6901 gaacaattcc ggaatcgcaa tacgataata agctgtttct ggaatacgat cgctgattac
     6961 cacatttgta aagaattctt tattgatttt caagaattcc acatctgagt tcattcgtgc
     7021 gtttttcact gagaagcgta aaagctgttt actttcaaaa tcgatatcat cgtcaatcac
     7081 atagaaccga acaaatttag acgggttgca attatctaat actgagacaa ataatgcagc
     7141 taaatgtggt acgaattttg tgttacaact tgcgactact gctatttctt ttttattcat
     7201 tgatctcgcc tactttcgtt agagtactgt gtgctaattt ttttaagtat aaattggtat
     7261 acggatgatc tttcaacgta ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag
     7321 gtttttcatt tccttcttta tactgacgtt catatttttc atttggtgct ggatgacgtt
     7381 caaaaatcag cgaagtctgc atgttccatt ttggatgaag ttgttcccaa tcttcataaa
     7441 gtacagcatt caaagcatct tggtcgtgat agatgattcg atcgccattt tcactgaggt
     7501 aatggatagt tttttctgta atttcttttt tattccattg atcaatgtca atcaccatca
     7561 ctcctgaatt gaagtacaag tcatcggaat cgattcctaa tctttccaaa gctttcactt
     7621 ggcctggatc gataatagcg ccgattgttt ttccatttaa cggctcatca tatagtttta
     7681 cgatatcgtc tagtactaac acatctgaat ctaagtaaag gactttctta taatcctctt
     7741 tagctaataa gtttggtaaa gaaattctta agtaagcggt agttgtgata tggtcactga
     7801 ctaggaagtc ttcgtagata tctttttcca ctgtcaggaa ttggatactt gcattacttg
     7861 aatatttatt taccgtttct tccaatcctt ctttactata ttctgataga ccatcgtcaa
     7921 tcacataaaa ttttatgcgt cttgttttat tgcaattttc taatgcagtt gcgatcatca
     7981 cgctcaagta aggagcataa ttttcgtctg aagcagttac cacaggaact acgccgtatc
     8041 tcttctccat caaaatcact cctttttcta atctttttct aacctgttct acatctagaa
     8101 tattcctcat ttggttttaa agcaaaatat atgctttgtt ttcattgaat attttaaaat
     8161 ttttttatca caaaacgcat cggtaagcgc taactattcg tttttcatgg ctttttattc
     8221 aaaggtattg accaaaaact actatatata gccacctttt tcttaagaat gttttttgaa
     8281 aaaattcata atgacaaagc aagttagttt cattttttta aattctcatt taatcttaag
     8341 gtaacaggaa aatagatatg taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat
     8401 tgatttagac ggtaccttgc ttgatcgaaa tggaaagata ccagaagaaa atatccaagc
     8461 acttcggaaa tttgatgaaa atggcggaat cgtaaccgtc gctaccggaa ggaacagcat
     8521 ttctgctaaa gatatttttt ccgaattagg aatcggtggc tatttgattt cttccaatgg
     8581 ttcactgatt gctgaaatga aggatggaaa aatggcgaaa gttctcagac gttcaaaaat
     8641 agatagcgat acactgagaa aagccttctt ctatgctcaa gaagcgaaaa tatccatcat
     8701 tgctagccga gaaacccaag atgatcagct gacattcgat gaatcaaatc tagtcaaaga
     8761 tgacccttat tatgaacatt tcaatttaca gaaccattct tttgatcaaa ttttaaatat
     8821 gttggaagat ccttctctta gctatcttaa attagcatta acagataaga gtgaagaaaa
     8881 actacagaag ctacaaagag atctgcaagc agatggtatc caatcagtat tctctgatcc
     8941 gcatttctta gaaatcacgc ctacaggtat cactaaagct cattcacttg tttttctggc
     9001 ggattacctt ggaatcgaat cgagtgacat catggctttt ggcgatcaag aaaacgacat
     9061 tgctatgttg aattacagcg ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa
     9121 agaaattgca aaccaagtaa cactgacgaa tgaagaagct ggtgttgctt atattttaaa
     9181 aaacttctat taaaaagaat aaaatgacga tatgaatgta ggaggaaaga aaatgttagt
     9241 tattattgat atgcaaaatc atattcttga tccaaacagc gaattttata ttccagagtc
     9301 agatcaattg gcagaacgta ttgctcaaag attagctaaa gcacgccaaa ataatgaata
     9361 tattctcttc acacgggata taccagttga actgaaggac aaagaagaag aacgagaaga
     9421 tttgcaattg atccctcttc tttctccaca agaaaatgaa cgagtgatta aaaagtatta
     9481 ctttacgatt ccacctgaaa ctttagctga aatccaggaa actttatttg aaagcaaaga
     9541 agaacaaaaa gagatcgaag tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct
     9601 gataggcctg cagagtgctt ttcctgaagc agactttttt gttgatccaa tgttagtctc
     9661 cagtagaaaa cacggagaat ccgctttaga gctgttgaaa gatttcaacg tctctatcgt
     9721 cgaatcataa aaatatctat cttcatttac gatatttaac acacaataaa aaaagcacgc
     9781 agaactgttt tagtcctacg tgctttttct atcaaagtag attggctttt ttatgacaca
     9841 gagtgaaacg gcaggctcac gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg
     9901 tattagctta acaaacggca aaatatgagg aactattttt ccgtttgtta agctaatact
     9961 caggtcaaaa cgctgatttc acaacctctt ccttaaacgg tattcaaaag cttgcatctg
    10021 cagtaataag aacagccaag taaaaacata agaagctgtt tttacttggc tgttcttatt
    10081 acttgatgca gggcagcttt ttcacaacct cttatgtacc ttgtaggact cgaacctacg
    10141 accggacggt tatgagccgt ctgctctaac caactgagct aaaggtacat cataaataaa
    10201 at
//
LOCUS       DAZKAS010000097        10192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000097 DAZKAS010000000
VERSION     DAZKAS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.099"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..625
                     /locus_tag="ACG8H7_002023"
     CDS             119..625
                     /locus_tag="ACG8H7_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFP8984099.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="ACG8H7_002024"
     CDS             642..1541
                     /locus_tag="ACG8H7_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFP8984100.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="ACG8H7_002025"
     CDS             1628..2512
                     /locus_tag="ACG8H7_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP8984101.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="ACG8H7_002026"
     tRNA            complement(2704..2775)
                     /locus_tag="ACG8H7_002026"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="ACG8H7_002027"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="ACG8H7_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP8984102.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="ACG8H7_002028"
     CDS             4318..5409
                     /locus_tag="ACG8H7_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFP8984103.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="ACG8H7_002029"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="ACG8H7_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP8984104.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /gene="pgk"
                     /locus_tag="ACG8H7_002030"
     CDS             6561..7751
                     /gene="pgk"
                     /locus_tag="ACG8H7_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP8984105.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /gene="tpiA"
                     /locus_tag="ACG8H7_002031"
     CDS             7855..8610
                     /gene="tpiA"
                     /locus_tag="ACG8H7_002031"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP8984106.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="ACG8H7_002032"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="ACG8H7_002032"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP8984107.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DAZKAS010000098        10091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000098 DAZKAS010000000
VERSION     DAZKAS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.100"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACG8H7_002033"
     CDS             162..392
                     /locus_tag="ACG8H7_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFP8984108.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACG8H7_002034"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACG8H7_002034"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP8984109.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACG8H7_002035"
     CDS             1803..1979
                     /locus_tag="ACG8H7_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP8984110.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACG8H7_002036"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACG8H7_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP8984111.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="ACG8H7_002037"
     CDS             2474..2941
                     /locus_tag="ACG8H7_002037"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8984112.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACG8H7_002038"
     CDS             3026..3910
                     /locus_tag="ACG8H7_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP8984113.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACG8H7_002039"
     CDS             4024..4260
                     /locus_tag="ACG8H7_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFP8984114.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACG8H7_002040"
     tRNA            4348..4428
                     /locus_tag="ACG8H7_002040"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACG8H7_002041"
     tRNA            4435..4506
                     /locus_tag="ACG8H7_002041"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="ACG8H7_002042"
     CDS             4631..4801
                     /locus_tag="ACG8H7_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984115.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="ACG8H7_002043"
     CDS             5021..5728
                     /locus_tag="ACG8H7_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8984116.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="ACG8H7_002044"
     CDS             complement(5788..6432)
                     /locus_tag="ACG8H7_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFP8984117.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="ACG8H7_002045"
     CDS             6559..6846
                     /locus_tag="ACG8H7_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984118.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="ACG8H7_002046"
     CDS             6954..7274
                     /locus_tag="ACG8H7_002046"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8984119.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="ACG8H7_002047"
     CDS             7355..8668
                     /locus_tag="ACG8H7_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP8984120.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="ACG8H7_002048"
     CDS             8665..9384
                     /locus_tag="ACG8H7_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP8984121.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="ACG8H7_002049"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="ACG8H7_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP8984122.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3363 a         1670 c         2152 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa c
//
LOCUS       DAZKAS010000099        10086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000099 DAZKAS010000000
VERSION     DAZKAS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.101"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..759)
                     /locus_tag="ACG8H7_002050"
     CDS             complement(247..759)
                     /locus_tag="ACG8H7_002050"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP8984123.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG8H7_002051"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ACG8H7_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP8984124.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ACG8H7_002052"
     CDS             complement(3212..3655)
                     /locus_tag="ACG8H7_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFP8984125.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ACG8H7_002053"
     CDS             complement(3656..4444)
                     /locus_tag="ACG8H7_002053"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP8984126.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG8H7_002054"
     CDS             complement(4441..5382)
                     /gene="rnz"
                     /locus_tag="ACG8H7_002054"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFP8984127.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ACG8H7_002055"
     CDS             complement(5559..6146)
                     /locus_tag="ACG8H7_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFP8984128.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG8H7_002056"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="ACG8H7_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFP8984129.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ACG8H7_002057"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ACG8H7_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP8984130.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ACG8H7_002058"
     CDS             complement(9063..9998)
                     /locus_tag="ACG8H7_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8984131.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAZKAS010000100        10010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000100 DAZKAS010000000
VERSION     DAZKAS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.102"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(474..698)
                     /locus_tag="ACG8H7_002059"
     CDS             complement(474..698)
                     /locus_tag="ACG8H7_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984132.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            889..3666
                     /locus_tag="ACG8H7_002060"
     CDS             889..3666
                     /locus_tag="ACG8H7_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP8984133.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3747..4166
                     /locus_tag="ACG8H7_002061"
     CDS             3747..4166
                     /locus_tag="ACG8H7_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8984134.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4178..4648
                     /locus_tag="ACG8H7_002062"
     CDS             4178..4648
                     /locus_tag="ACG8H7_002062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8984135.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4665..5444
                     /locus_tag="ACG8H7_002063"
     CDS             4665..5444
                     /locus_tag="ACG8H7_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8984136.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5431..6252
                     /locus_tag="ACG8H7_002064"
     CDS             5431..6252
                     /locus_tag="ACG8H7_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8984137.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6269..7318
                     /locus_tag="ACG8H7_002065"
     CDS             6269..7318
                     /locus_tag="ACG8H7_002065"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8984138.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7331..8335
                     /locus_tag="ACG8H7_002066"
     CDS             7331..8335
                     /locus_tag="ACG8H7_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8984139.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8968..9987
                     /locus_tag="ACG8H7_002067"
     CDS             8968..9987
                     /locus_tag="ACG8H7_002067"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP8984140.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3360 a         1650 c         2093 g         2907 t
ORIGIN      
        1 gcaatctttt ttgctacgta atttttgtaa aaaagtgggt tgagagaaat ctaattgatt
       61 ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg tttattgtgg gtactatctt
      121 agaggaataa ataatagtgg agaaaactat ggtttggcgc agattctatc aggtttgatg
      181 gaacgaagac aacctcccga catcaagtca aaagctactt gagattttga tctgaaaata
      241 aaaaaagaaa acaaggtagg aacatcaatg tttctacctt gttttcgtaa tcaatgggaa
      301 aaaactaaga gtggtgtacg taatcaataa aaaggatggt gacaacagta cctctttact
      361 ctctgaaagc atcataactc gtttttcctc attatgccaa taaattgttg ttataccaca
      421 tataaggatt tgtaatagct aaaaacagac aggaaagttg aaatctgtga atcttatttt
      481 gtatggaagg atttatagaa cgcaaaaatc aagataaaga tgaataaaaa tcctgtttcg
      541 gctatatatt gcgtatctac gccaataaaa cgagtttgtt ctgtcatagc catataaagc
      601 aaaggcaaaa atacaactac agcgagataa gatccgatga tttctcgaaa agttttccag
      661 gaagtatgtt tcctgttttt atatttatcc gatttcattg aatagtcata gcctccttat
      721 tctttcatat gttgaaattc cagtataaaa actggaagga actgtacgtt ttttagaata
      781 aaaattataa gggataagta ggctgctgtc tataataaga tatatttttc aattctttat
      841 taaagatttt attgacaaaa tgaatccgtt ttctatgatg gaggaggggt gaataagttg
      901 ttgaagaagg aaattttatg ttacttagag aaccagacag cctttttcga tttagaacat
      961 atgaacgagg tctttacagc acaaaggtta gctgatcatt ttggggtaaa aagaaacaca
     1021 gtcagtcatt atttgaacca gttaaatgat gagcaactac tagtcaaaat caattctcga
     1081 ccagtgattt atttccacaa agaagctttt gaaaaacaat tttttaagtt acgcacgaat
     1141 ttttattttt ctatcgatga tctcaaagag gaacagcctt ttttcgcgca accaaaagac
     1201 ttattctctt ttatgatcgg atacgacgcc agtttgaaag aaagtatcga acaaatcaaa
     1261 acagctcttt actatccaga tggcggatta ccacttttga ttacaggcga aagtggcaca
     1321 ggtaaaagtt atttagttaa tttggtctat cagtattgtc tgcttcatga tctgctagaa
     1381 gattctgcac cattcatcac ttttaattgt gcacaatatg cagataatcc tgaacttttg
     1441 actagcaatt tgtttggaca tgttaaggga gcgtacactg gtgcagaaga aaataaaaaa
     1501 ggggcttttg aagcagcaga tggtgggatc ttgtttttag acgaggtaca tcgcttaaca
     1561 ccagaaggtc aggaaaaact gttcacctat cttgatcaag gtgtgattta tcgtatggga
     1621 gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt ttgctacgac tgaagagatt
     1681 actagtcatt tcttaacaac tttcattcga agaataccta tccgaatcga actacctgca
     1741 cttaatcagc gtagtcgtaa tgaacggcta gaactgattt actctttttt tctcgaagaa
     1801 caaagaaaaa tggggagccc tttgtcagtc agtggaccag ctttgtcact tttaacaagt
     1861 cagaaattat ccggaaacat aggagagctg aaaaatatcg tcaaagtttc agcagcaaaa
     1921 gcatatgctg aacagcgtga acagcctgaa atccatgtca cgatccatca tttgcaaaaa
     1981 gaactacttg cgcagcctat tactaaaaat acagcacagc aaggaatcta tattacgcaa
     2041 gaccagacct tagagcaatt aatggagaag caacagcccg aacagcagcg aatcgttaaa
     2101 agttttgagc aaatcctaat tgatttcaag aaaaataact gtctgctata ttcttgcgaa
     2161 ggaaaattaa agcaagaagt gattcaatta tttgattttt tgctgtttga gaccagtaga
     2221 caagaaaaac acgagctgct ggtttattgg acacaatcta tccgagagac ctttcgacaa
     2281 atggaaactg cctatcaaat caaattcgat ggaaacagtg tctatgcctt gagttactat
     2341 ctctatcagc gaagcactat caaatggtta ccggaagaac atgatattat ccaactaatc
     2401 aaagaattag aacaacaagt ttctcagtct tatccatcta gttatcacta tgtccaacga
     2461 attctggaat taagtaagat caaattagat attgaagaca caagtatgga tcggatttta
     2521 ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa aacgactgcc aaaagcgatg
     2581 attgctgctc acggttatgc aactgctagc agtatggcaa atgtagtaaa tcgtttatta
     2641 caagaggatc tatttgaatc atttgatatg ccactagatg tcacaccaca gcagattgct
     2701 tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa atggattgat catattagta
     2761 gacatgggat ctctgaaaga aatccatcag ttttttaaaa aacagttatc cgtaccgctt
     2821 ttgattttga ataatgtgac gacaccttta gcaatcacag taggggaatg cctgcaaaaa
     2881 aatgtatcat tagaagagat agcagaagag actgttcgac agatccagcc agaatggcga
     2941 ctgctctatc cagaggaaaa caaacccaaa gcactcatta ctacttgctt cactggaatc
     3001 ggtacggcgg tccacttaag tgaactgcta gaaaaaagct tacctacggc ttgtcagttg
     3061 aaaatcatcc catatgaata tcagcaatta aaagataaga aaaacagtga cccattattt
     3121 tccatatacg aagtcgtagg aatgattggc accacagatc ctgatatcac caatatcgac
     3181 tatctttcat tagaagacct catctcgggg gaaaagatgt ctgtactagc tgaatggttg
     3241 gacactacta tgaatgcttc agagaaggaa atattcaatc agcgcatcat tcgtaatttt
     3301 tcattggaaa aagtactgga ttccgtgact attctagata cagagaaggt gatgggagaa
     3361 atcgatcatt ttatgagaga attagaggtg cagttgaatc gaagcatcag caatcccaaa
     3421 aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac gattgatccg acaaatgcca
     3481 atcgaaacct atcaaggatt agagaaatgg aagcagtgtc aaaaagaagg gttatctgct
     3541 attaaatctg cgtttagtgt cattgaagag cattatagtg tcatgatccc caattctgag
     3601 attgcctata tctatgatat tttgagtgaa gatacagagt ttctctcgat agaagaagaa
     3661 ttttgaacat aagcaagttg ttgccactaa ttttggcatg caacttgctt ttttagtatg
     3721 tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat tatcttagct tcccatggaa
     3781 aatttgcttc agggatatta gattcattag agctgatcta tgggaaaaat cattctatta
     3841 ctgtattcga ttgctatact gatgagaatt ttgatttagc ggaaacagta aggcagcttt
     3901 ttatgaggta caaagataaa gaaatcattg ttctgacgga tctttttggc ggaagtgtca
     3961 acaacgaatt cttacaatat atcaatcggg atcatgtcta tctcgttgcc ggattgaatt
     4021 tgccactatt gattgagcta gtatctcggt tggacgctga agtagagaca actgttttga
     4081 tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt ttgtaacgag cggctcaatc
     4141 agaaaataga agaagacgaa ttttaggagg aagaaaaatg attgtattga caagagtcga
     4201 ccatcggtta ttacacggac aagtagcttt ttcatggaca caaacaatag gcgcagactg
     4261 tattttgatt gctaatgatg atgtacctac gaatgagata cgaaagacaa cgatcaaatt
     4321 agccaagcca caaggagtca aactagtgat caaatcaatt gatgactcga ttgccgcgtt
     4381 gaaaagcggg gtgacagata aatataagtt attcatcgta gtggaatcga tcgaggatgc
     4441 atataagctg gcagaagcgt atccagaaat caaaaccatc aacttaggag ggatcaaggc
     4501 aaaacctggt actcgcagca tcggaaaagc ggtaaatatt ttaccagaag aggaggaacg
     4561 cttgaaacga ttaattgaca aacaaataga agtagaaatc cgtcaagtac cagcagataa
     4621 aaaagtggat gtaagagaag ttttgtaaga aaggaagaac ctcaatgatt gtacaagcag
     4681 tcttattagg aattgttgca tttgtcgcac aatcagaata tgcactaggg acatcgttgc
     4741 tttctcgtcc gattgtgacg gggctgttta caggaatcgt gttaggtgat gtaaaaactg
     4801 ggatcattat gggcgcaact ttagagttag catttatcgg atcgttctca gtaggtgctt
     4861 cgattccacc tgatgttgtg accggtggga ttttagggac ggcttttgct attacagctg
     4921 gagccggtac agaaaccgcg cttttactag gtttgccgat tgctacgttg acattgatat
     4981 tgaaaaatat ttacttaggg cttttgattc caattatgaa ccataaagca gacaattatg
     5041 cagaagaagg aaattataaa ggagtcgagc atatgcactt gcttgccggt atcggtcttt
     5101 ccttgatgct tggtgttgtg gtaatggttt cttatatggt gggaagcaat acgatcggaa
     5161 atctattaaa tatgattcct gacttcgttc aaaaggggtt gtcagtagct acagggttga
     5221 ttccagctct tggttttgcg atgcttgctc gtttgctgat caacaaacaa gttgcaccat
     5281 attttttctt aggttttgct atagcttctt acttagagat tcctgtgacc ggagtagcta
     5341 tttttggtgc gattttagca gttgtggtag tcaatattat gaatgtccgt caactaccac
     5401 ttcaaacaac tgcaggggag gtagaagaag atgaagaatt ctgagatcaa aaacagcaaa
     5461 ataacgaaaa aagagctaaa tcacgtattt tggcgttctt ttcaaatgga gttttcttgg
     5521 aattatgaac gtcagatgaa tatggcttat gtttatgcaa tgattccgat tttgaaaaag
     5581 ttatatcagt cgaaagaaga aatggcagca gctttgaaac ggcatctaga gttttttaac
     5641 acgaccccac atatcgttac actgatttta ggaatcacgg ctgcaatgga ggaagaaaat
     5701 aaggatgatc ctgaatttga tgtgactgcg atcgatagta tcaaaacatc tttgatggga
     5761 cctcttgcag ggttaggaga ttctttcttt tggggaactt tgcgattgat tgccacaggt
     5821 gtgggtacct ctttagccat gcaaggaaat attttaggcc ctatcttgtt cattttgatt
     5881 ttcaatattc cgcacattct gtttcgctat ctagcaacag gttggggata tcgcttagga
     5941 acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg aaagtttgac attaggcgcg
     6001 tctattattg gactgatggt cgttggagca atgactgcca cgatgatcac catcaatatt
     6061 cctttaaaaa tcggttcagg agaaaatgct gtgactgttc aaagtatttt tgatgatatc
     6121 gttccaaata tcttatgtct tggagcattt ggcatagtgt tttatctatt gaagaaagaa
     6181 gtgaaaccat taacgatctt gatcggacta gctttctttg gaatttttgg ttcgtggata
     6241 gggatttttt aaaaaaggag cagagaaaat gtttacgatg caagattata tctatgaaga
     6301 aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt tcgacaagag aacatatgaa
     6361 aaaaacagtg aatttactga tcttagcaac gggttcatct tataacgctt gtttagcagc
     6421 gaagccagca ttggaatctt atggtgatct tactgtcgat attcaagaac ctttttattt
     6481 tttcaattat ggaaaacttt ctccttcaat cgatactgtg attgctgtgt cacaaagcgg
     6541 gaaaagtgct tctactgttg aagcagtaaa aatgatacaa aaacaagggt tacctgttgt
     6601 tgctatcaca aatgacgtgc aaagtccact tgctttagaa gctgcacaaa ttattgattt
     6661 aggtgccggg gtagaatcag taggcttcgt tacaaaaggc tattctgcga ctgttttaca
     6721 gttactactc ttagggatag gaattggaat aagtaaaaat aaaatcagca aaaaaataga
     6781 acaagactac atgcaacagt tgcgcaaaat aatcaaccat ttaccagcga ttattcaaaa
     6841 aacggaagaa ttttttgatg gatatcaaag tttatttcgt ttagcacagc gtttcgtcac
     6901 cattggatat ggtccaaact ggggaacagc taaagaagca gagacgaaat tgactgaaac
     6961 aatccgcgtt ccgtcacaag gatttgagtt agaagcttat atgcacggcc catacctgga
     7021 ggcagatgct tcgcatttgc tattctttat tgaaggggaa agtgtaaata aggaacgttc
     7081 gcaaaagttg caaagatata tgagtcggta cgttggagag actctcacca ttaccacaaa
     7141 aaaagcaaga aacgagaaaa cacttggatt agcaatagaa tgtgatgaat atctgtctgt
     7201 attagcgctt gtcgttccgt ttcaactatt cgcttataaa accgcagtag ctaaaggtat
     7261 tgacttgaac aaaaagattt ttgaagactt tgatacagtc ttgaaaagta aattataaga
     7321 ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc aaagcaataa aaggcgcact
     7381 agctttacgt ccgcaagtag aagaaatcat cgataaactt tatgcggaaa aatttgacgc
     7441 tgtctattat ctaggaatcg gtggaacgta tgcttcctcg atgcaagctg tcacttatat
     7501 gaatgggaaa agcaacttgc ctgtctttgt acagcatgct gcagaatatt acactactgg
     7561 aaacaaaaga ttaacaaaag attctatcgt tattttgtcc tctgttaccg gtacaactca
     7621 agaagtagtg aaagcagtag aacaaatcaa gaaagtagga gcgacactga ttggatttat
     7681 cgataaagcg aacagtaagt tgtctcagct atgcgatttt gtggtcactt atccagctcc
     7741 tggaacagaa caaatcaaat tttttatggc agcagatcga ttgatgtatt tgaatggaga
     7801 attcgaagct tattcagaat actatgaaca gctcgaacgt tatttaccaa cagggttagt
     7861 ggaagcagag aaaaaagcag atgcatttgg cttatcattt gcagaaaaac atcgacgtga
     7921 tagtatgcat tattttattg gagcagggaa tcaatgggga gctgtttatt catatgccat
     7981 gtgttactgg gaagaacaaa gctggcttcc atcgaaatct atccatgctg ccgagtttct
     8041 tcacgggacg ttagagatcg tagaagaaac aaccccagtc acattatttt taggcgaaga
     8101 tgaacagaga gtgttatctg aacgtgtggc taaacttttg ccaaaaattt gcagcaatta
     8161 tacattgata gacacaaaag actatccagt agaaggtatc agtgaaaaat atcgcggacg
     8221 tgttttgtct ttcttattga tgcatgtagt gacacaacgc atcgatgctc atgtggagca
     8281 gttgaactgc catccattag agatacgccg ctactatcgt cagtttgatt attaggatag
     8341 actggataaa aagaatataa aaaataaaga gagcagcgat aactgctccc tttatttttt
     8401 taaaatattg gcatcaatta cgttataaaa cacatttttt gcattatcta cagttcaact
     8461 tataatctgt ttaatgggac atgtgggtgc caaccatttt ttgttatgca atagtactat
     8521 ttagttgttg catggatccg tagtaaaagt ccaagtacaa cgagttgcgc cagtttacat
     8581 agcgttttgt atccagaatg ttgactaaaa caaaagttcc tatgtcaacc gtcaacagag
     8641 acaatctgtc cgttgcagga cctgacagat ttagaaatgt tcaatctgtc tcttatgtat
     8701 tttaaatcaa tactcactag gagaagtttc ctaacctcct aatagtaatg ctggtagtaa
     8761 cattgtcaaa actgcttcct cttaggaaca agcaaagcat aacgagtgta aaaataaaat
     8821 gtagactatt gtaacatatc attgattatt tcaagtaaaa acgataaaag aagtaaacaa
     8881 acacttaaaa acaatggaaa ttagtcccaa ctttaagaaa tgtatactat aatggaaatt
     8941 atcaattatt tatttggagg aatatccatg aaacaaaaaa tcgcagttct tggtcctgga
     9001 tcttggggca ctgctttagc acaaacattg gcagaaaacg ggcatgatgt ccgtatttgg
     9061 ggaaatgtac ctgaacagat tgatgaaatc aataagtatc atacaaatca gcatttttta
     9121 cctgatttga cgatcccaga atcgattatc ggatataaag aattggcaga agcagtggat
     9181 aacgcagatg ctattttatt tgttgttcca acaaaagcga ttcgttcagt cgcacaggaa
     9241 cttattggta aaatgaatac taagccaatc attatacatg caagtaaagg actagaacaa
     9301 gatacgcata aacgaatctc agaagttttg gctgaagaaa ttccagaaga aaaaagacag
     9361 gcaattgttg tattatctgg tcctagccat gctgaggaag tggcagtaca tgatatcaca
     9421 acaattacag ctgcaagtat tgatcaaaaa gcagcagcct atgtacaaaa actgttcatg
     9481 aatgattatt tcaggattta tacaaataat gatgtgattg gcgtagaaac tggtgcagca
     9541 ctgaaaaata tcattgcaat tggtgccggc gctatccatg gattaggatt cggagatgat
     9601 gcaaaagcag caatcatgac tcgaggattg gcagaaatca gccgtcttgg tgtcgcgatg
     9661 ggtgccaatc ctctgacatt tatcggtttg agtggtgtgg gggacttgat cgttacttgt
     9721 acaagtgttc attctcgcaa ttggcgtgcc ggtaacttgt taggacaggg acataagctg
     9781 gaagaagttc tagaaaacat gggaatggtc gttgagggtg tggcaacgac aaaagcagca
     9841 gtagagctag ctcaacagct aggtgttgag atgccaatta ctcaaacaat ctataatgtt
     9901 ttgtataatg gagaagatat caaaaaagca gcaaaagaaa tcatgttacg tgacggaaaa
     9961 atggagaatg aattttctct tcgttaaata gatgatcaat agttaatcgt
//
LOCUS       DAZKAS010000101         9965 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000101 DAZKAS010000000
VERSION     DAZKAS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.103"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..720
                     /locus_tag="ACG8H7_002068"
     CDS             241..720
                     /locus_tag="ACG8H7_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFP8984141.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            753..2981
                     /locus_tag="ACG8H7_002069"
     CDS             753..2981
                     /locus_tag="ACG8H7_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8984142.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3110..4753
                     /locus_tag="ACG8H7_002070"
     CDS             3110..4753
                     /locus_tag="ACG8H7_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP8984143.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4826..5371)
                     /locus_tag="ACG8H7_002071"
     CDS             complement(4826..5371)
                     /locus_tag="ACG8H7_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFP8984144.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5394..5879)
                     /locus_tag="ACG8H7_002072"
     CDS             complement(5394..5879)
                     /locus_tag="ACG8H7_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP8984145.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5869..6681)
                     /locus_tag="ACG8H7_002073"
     CDS             complement(5869..6681)
                     /locus_tag="ACG8H7_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFP8984146.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6820..8352)
                     /locus_tag="ACG8H7_002074"
     CDS             complement(6820..8352)
                     /locus_tag="ACG8H7_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFP8984147.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8461..9492)
                     /locus_tag="ACG8H7_002075"
     CDS             complement(8461..9492)
                     /locus_tag="ACG8H7_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8984148.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3316 a         1844 c         1915 g         2890 t
ORIGIN      
        1 tggtgaagtt caccaacatc aaaaagtata gagccaaaat attccaacat catcttggac
       61 tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat
      121 gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt
      181 aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata
      241 atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa
      301 gttgatggaa gaaatgcatt aaatatggat gcaaaagggc tttcactggg ccttgctcag
      361 tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa
      421 tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct
      481 gcgcaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa
      541 aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc
      601 aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga
      661 gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa
      721 tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc
      781 gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa
      841 gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga
      901 aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag
      961 cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca
     1021 aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag
     1081 aattactaga agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga
     1141 accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga
     1201 tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag
     1261 cgattgaaaa aggctattta ttagagattc aagagccgac tgtcatccga gatgcctctg
     1321 ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa
     1381 tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa
     1441 atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc
     1501 ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac
     1561 ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca
     1621 tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac
     1681 aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg
     1741 aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca
     1801 tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagaa agaaccatta
     1861 gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag
     1921 atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa
     1981 caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag
     2041 gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca
     2101 tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga
     2161 aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa
     2221 cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc
     2281 aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag
     2341 gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga
     2401 acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa
     2461 tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt
     2521 ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag
     2581 aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa
     2641 aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag
     2701 cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc
     2761 agccaaaagc attgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt
     2821 tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag
     2881 aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat
     2941 ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa
     3001 cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta
     3061 aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga
     3121 acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag
     3181 caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga
     3241 tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg
     3301 ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg aagattttga
     3361 tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa
     3421 ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta
     3481 tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc
     3541 gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt
     3601 ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt
     3661 caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc
     3721 tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca
     3781 aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga
     3841 tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg
     3901 gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga
     3961 aaaagaaggg caattagatg tccttaaatt gaaacatgca ttaagcgctt ggcaaacttc
     4021 attggatggt atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt
     4081 ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat
     4141 gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac
     4201 gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa
     4261 aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac
     4321 gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac
     4381 acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga
     4441 atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa
     4501 taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt
     4561 ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact
     4621 tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc
     4681 ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa
     4741 aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt
     4801 cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag
     4861 gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag
     4921 gtacttggac atgcttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt
     4981 gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac
     5041 aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg
     5101 ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg
     5161 caatttcttt aggatccaac aactctttta acgtacggat gatccattga ccaacttccg
     5221 cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa
     5281 aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt
     5341 ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat
     5401 tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca
     5461 atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa
     5521 atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat
     5581 ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg
     5641 atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca
     5701 ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc
     5761 ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat
     5821 gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt
     5881 tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag
     5941 cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa
     6001 atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt
     6061 tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag
     6121 aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt
     6181 tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc
     6241 ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa
     6301 aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa
     6361 tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac
     6421 aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat
     6481 tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa
     6541 tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa
     6601 attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc
     6661 aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc
     6721 aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa aaacaaaaag
     6781 aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt gtgattttaa
     6841 ttctttctcg atttgaagcc gttgcacttt cattgtagct gttcgcggga ttgcatcaaa
     6901 atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc accatcgatt
     6961 ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat gatttttatc
     7021 ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt cttcaatagc
     7081 taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa ataggtggcc
     7141 atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa cattttcctg
     7201 gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta attgtatatg
     7261 accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag cgcttgtcat
     7321 gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct ctagtgtatg
     7381 agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa ataccgcatc
     7441 ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag catcaaaagt
     7501 ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag taaaacgcca
     7561 ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt ccaacatatg
     7621 aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca tggacatcaa
     7681 agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag cgattaattt
     7741 cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc ccatagaatg
     7801 agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg tcatataaga
     7861 aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt ccattaaata
     7921 aagaatagga atctgttttg attttgttcc atttgaaatt tctctcactc gcttttttgt
     7981 ctcatcatca aataaaatga aagggtcttc taaacgatcg ataaatactt ccattgttgt
     8041 tgttggtaga tgataagaag tcatgacagg cactgcacca aggtaagaca cagaaacagc
     8101 tagaaggtat gtgtcaaaag cggaactctt ataaataata actttgtctc cggatttcac
     8161 tccaagatga gccaattgat aagcccgttt caacacactt tcgcagcatt ttttgtaagt
     8221 tgtttcaagt cctaattctg ggaaagctga aagtggctca tcaaagataa tcggggtctc
     8281 ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag ttcaaagggg aatacgttat
     8341 ggtctccatc attcacactc cattatcttt tgttaagtaa tatacaaatt caagataatc
     8401 gtaacataat cctttatata ataacatagt ttattgtgaa aaaaagatca tacatttgtt
     8461 ttatatggaa gaaacccgaa cgaatgccgc taattcgtga taagaaaaat caaccatctt
     8521 ttcccagata tcttgaaaag aatgggatga gaggtatcga ttaccacata aataatttac
     8581 cgcgtcagca tcatctaaat atagttctgc ataataacga tatgtttttc tgcatagttc
     8641 tggcgatacg tttttatata ctgtaatttt gctcaaaatt tggcgaatag tccgaggaga
     8701 aatttgctga tcatgtgagg aacgaaaaac aaaagaattt tcgtccgtat tttgtgctgt
     8761 taacttaaaa tagttctcca gaaatggcat cgcttctttt ggtattggta ctttattcag
     8821 tctgttttct tttctattca gattaagaat ctgcttggtc aaagatacat ctttacgttt
     8881 aagttctact acttcatgga tctttaatcc gcaatacaga agaatggcag taattgcaag
     8941 gttcctcggt ctattctttt cttcaaagct agcttctctg cttgtttgaa agccttcaat
     9001 cgggataaac aaatgctcaa aatctttgaa aagttctgca tcgatatatc gcggagctgc
     9061 tttggttgtt tccggtaaac ttgtacccaa aagagaaatc tcttcttcaa tcagtccttt
     9121 ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc ttccttgaaa tcccgtaata
     9181 gctattttca aacaattgct ccgtttcttg gttcgctgta gtttggaacg aacgagtatg
     9241 tcgatagatt ttagctaaga aattctgata atcttgtttt tctaaatgga taatcagctg
     9301 atcatccact tcttcagaaa atacttttct caaataatca gtaaacagat gaaaatcttt
     9361 tgcgtattca tagcgactgc ctaatgaaag ggaagaacga ttttcttcta aaaactgttg
     9421 aaagacggaa gggagcaagt gtgtacgttg ctttaacttt tcttgcagtt gttttctagt
     9481 tttgctgtcc attacaaatt ccctccaata gtgttcattt tctatatttt accgcatttc
     9541 atcaaatgaa gatagacagt ttgtcttgat cttaggaagc aaatttttaa ctaagtacta
     9601 gctaaaagat atgaagtaag aagatacatt tttagttatt ttattagtgc agtcattctt
     9661 ttgatctttt aattgattta tcctcttttt agctatgtgg ttacttcatg agcaatgaaa
     9721 tggatttggt tcgttctaat ggcaggaggc tctttctttt atttccgaaa aattagaaaa
     9781 cattttttca gaatcgattg gctctccatt tattcaataa tggtttgtct aacgatattg
     9841 ttgggataat tcttaaagaa gtattagaaa tagttggtac ttcattactt tacatgaaga
     9901 tttattctat ggtaggagca attatattga gaattaatcc ttttttatat aagttgtttg
     9961 cttat
//
LOCUS       DAZKAS010000102         9831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000102 DAZKAS010000000
VERSION     DAZKAS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.104"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..205)
                     /locus_tag="ACG8H7_002076"
     CDS             complement(<1..205)
                     /locus_tag="ACG8H7_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984149.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="ACG8H7_002077"
     CDS             complement(221..835)
                     /locus_tag="ACG8H7_002077"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP8984150.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="ACG8H7_002078"
     CDS             complement(982..1665)
                     /locus_tag="ACG8H7_002078"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP8984151.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="ACG8H7_002079"
     CDS             complement(1774..2232)
                     /locus_tag="ACG8H7_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP8984152.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="ACG8H7_002080"
     CDS             complement(2316..4079)
                     /locus_tag="ACG8H7_002080"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP8984153.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="ACG8H7_002081"
     CDS             complement(4300..5760)
                     /locus_tag="ACG8H7_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984154.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8H7_002082"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="ACG8H7_002082"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP8984155.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="ACG8H7_002083"
     CDS             complement(6917..8344)
                     /locus_tag="ACG8H7_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8984156.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="ACG8H7_002084"
     CDS             complement(8375..9589)
                     /locus_tag="ACG8H7_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFP8984157.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2917 a         2041 c         1629 g         3244 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa g
//
LOCUS       DAZKAS010000103         9646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000103 DAZKAS010000000
VERSION     DAZKAS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.105"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(30..692)
                     /locus_tag="ACG8H7_002085"
     CDS             complement(30..692)
                     /locus_tag="ACG8H7_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984158.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(705..2207)
                     /locus_tag="ACG8H7_002086"
     CDS             complement(705..2207)
                     /locus_tag="ACG8H7_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8984159.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2755..4434
                     /locus_tag="ACG8H7_002087"
     CDS             2755..4434
                     /locus_tag="ACG8H7_002087"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP8984160.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4561..5040
                     /locus_tag="ACG8H7_002088"
     CDS             4561..5040
                     /locus_tag="ACG8H7_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFP8984161.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5259..5939
                     /locus_tag="ACG8H7_002089"
     CDS             5259..5939
                     /locus_tag="ACG8H7_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP8984162.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5947..7278
                     /locus_tag="ACG8H7_002090"
     CDS             5947..7278
                     /locus_tag="ACG8H7_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFP8984163.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7293..8255
                     /locus_tag="ACG8H7_002091"
     CDS             7293..8255
                     /locus_tag="ACG8H7_002091"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFP8984164.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8366..8704
                     /locus_tag="ACG8H7_002092"
     CDS             8366..8704
                     /locus_tag="ACG8H7_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP8984165.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8783..9490
                     /locus_tag="ACG8H7_002093"
     CDS             8783..9490
                     /locus_tag="ACG8H7_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFP8984166.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3061 a         1736 c         2073 g         2776 t
ORIGIN      
        1 gattttttat ttgatcatta tcctgttttt taaacttcga ttttttgaaa ttttttattt
       61 tttagttcaa agacttcatc acagcttttt gctacttcaa tctcatgagt cacgatgata
      121 atacagcgat tttgttcatg ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt
      181 gtttcatcta gatttccagt aggctcatcc gctacgatta gttgatgttc acagcagata
      241 gcacgaacga tagcgactcg ctgctgctga cctcccgaaa gtttggtcac ctgttttttt
      301 gctaatattt catcaattcc tacacgagcc agtcgatcta aagcaacttg tcgataattc
      361 tcttgcttgg tcttagtgat ttccattgct gctatgacgt tgtctagagc cgataaataa
      421 ggaagcaagt tgtaagcttg gaaaataatc gagacatcat ttttacgata acttcttaac
      481 ccgattttgt tcagtgaacg atcttgatag tatacctttc cttcttttgg tgcatctagt
      541 ccagaaataa gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa aatagcgtac
      601 atttttccag cttcgaaatt cagttctaca tctttataaa gcgcatcttg cggatcttga
      661 taccaaaatc ccaattgttc tgtccgtaac atcaaattcc tcctttagtt taataggatt
      721 tttcttgggt ttaatcttaa tatccctata gaagctaaaa agacagaaaa cagactgatt
      781 ccaaatgcta ctccggctaa tagtcccaat tgtgcaggtt tgacattgat ttccaattcg
      841 ctgacttgat cagaaacagc aaatggatta ttcgtggttc cttgttgacc atcgcgttga
      901 ccgccttgtt cttgtctgtt acctggcatt tgtccaccgc caggagcttg ctggttttgt
      961 gtcgtggttt ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc gacgacgttt
     1021 ccgctaagcg aagcaactcc taatgcaaga atcatacaaa tagccacttc ggtaaacaat
     1081 tggagaatga ttttacttct ggattctcct aaagatagca agacgcctaa ctcatgccga
     1141 cgctcccgaa tggtaatcat aatgatcaga gtaagtatga taataccggc tacagccact
     1201 agtaaaacaa catttttaga aaaacttgcc acgttgttta aaggttccaa cattgactga
     1261 tacatggaat catttgattg taagctaaat gtatctgtgt cgatcagctt ctcagcttct
     1321 ttgagaaact catccatttt atctggatca gatagagtgt aaatagcaga atctatcgtg
     1381 ttatcgtcag atgtcccatt tagttcattt gcaaaagtgt aagaagtgta aagtgtattc
     1441 gacggattca tgaagttgaa tttcataccc atactgccgc ttgtttcgct ggattcataa
     1501 attcctttga tcgtcatttc ataagtggta tcttcatctt cagtactcgt gattgtgaaa
     1561 gtatctccga ctgccaggtc atttgtttct gctagagtag aatcaatgac tacattgttt
     1621 gtgcctttgt cgctgtcatc tattccttca ccatccgtga tttttgctgt tccatcagaa
     1681 aaattactat tttgtgcagt tgctgacaca ccactgattt gaaagtctgc ttgagtcatt
     1741 tgcggattcc cgcccattcc tccagcaggt tcaccgcctg catcagtagt ttctgaactg
     1801 tcttcacttg aatcagaact agtgatggca gttattccat cttttgccat agctgaggcg
     1861 gaactttcaa aggaataact ctttacatta tctaattcag ctattttttc cgcgtccgtt
     1921 aaggaaactg gtgtaagaga aaaactgcct ggatctggac gttcttcagt ggtagaaccg
     1981 cttgaagaag tttgttcctg ttttttaaat gaagcttctc gattggcact taaagtcact
     2041 gttgctccaa cttctttttg tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg
     2101 aggccagcta gcacaaaaat aagaatggct gtgaacacgg caaacaataa caatgaacgt
     2161 ccccatttta cttttgtact tttccatgca cgtttcataa aattcatatt ttgcatccct
     2221 cctagtagat gcatttgata atagacaacc tatcttaaag ctagcttaaa tcttatgctg
     2281 tttgcacacc atcaacatga ggttctgtat atataaaaag tcactatagt tattgcaaaa
     2341 aatgtggtaa aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga aagaggaata
     2401 gcaaaagaaa tcctagatgc tacagaggga tttttacgtg catagttgtt ctaacaattg
     2461 ctgatactag cgatttagtt tatttgttgt ataataatgg ggaacagctg aatacttata
     2521 taagtattct ggctatagcg tttcgcaaaa gatccatttt gttaatgaag tatatccaaa
     2581 ataaaatgcc catccaactg tcagaacgcg gatgtgctgg taattttcct gctgattctc
     2641 cataagttga gaaatagtga ttttgcatat gcttcattgg aatggctcat atatttagat
     2701 aaagaagaaa atcgacatct ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt
     2761 accattaaaa ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca
     2821 gaagtgggcg aggatatttt tattttagat tgtgggctaa aatatcctga aaatgagttg
     2881 ttggggatcg acgtcgtaat ccctgatttt acgtatttag aagaaaacat tgatcgtgta
     2941 gctggtgtct ttttaacaca tggacatgca gatgcgatcg gggcacttcc ttacttgttg
     3001 tcaaaaattt ctgtaccagt attcgggaca gagttgacga tcgagttagc aaaattgaat
     3061 gtcagtcgta atgaagcagc caaaggattt aaggatttcc atgtggtaga tgagcataca
     3121 gaaatagact ttggtgaaac agtagtgagc tttttccgta ccactcatac gataccagat
     3181 tcagtgggga tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg agattttaaa
     3241 tttgaccaaa cggctatccc aatgtatcaa acagattatg cacgattggc ggaaatcggc
     3301 aaagaaggag tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta
     3361 gcttctgaag cccaaatagc agatgaagta tttgatacga tcaaatattg ggaagggcgt
     3421 atcatcgtcg cttgtgtagc aagtaatttg caacgagtgc aacaagtatt ggatgcagca
     3481 gaaaaagcac aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca
     3541 gcgatgcgtt tagaaaaact tcaattacca agtgaagatc tgcttgttac gttgaaagac
     3601 atgaaaaact atgatccaga agaactgatc attttagaaa ctggccgaat gggtgaaccc
     3661 atcaagtctc tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat tgaagaagga
     3721 gatttggttt atatcactac tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg
     3781 gaagatatca tttatcgagc tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt
     3841 tctggccatg ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa accaacatat
     3901 tttgtgccag ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt ggctcatgca
     3961 gtagggatgc catacaaaaa tatctatatt actggacgcg gagacgtgct ggaatataag
     4021 aacaagcgta tgagtgttgc aggaactaca agtgcagaaa atgtaatgat tgatggatta
     4081 ggcataggtg atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc agaagacggg
     4141 attttcgtag cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa
     4201 attacttcac ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc
     4261 aatatgattg aaacaatcgt tgagaaacat ttagaaagca atgattttga gtggagcaaa
     4321 ttaaagcaag acatccgaga tcaattaggc cgttacttat ttgaacaaac aaaacgccgc
     4381 ccagtgatct tgccagtcat tatggaagct actcaacgtc gaaataaaaa ataaacaaaa
     4441 agagtaagat gaacctctct atagagtgct cgtcttgctc tttttttgcg aaaaacaaat
     4501 aatttttgaa tacagcatcc ctcgctttat aataaacgat agaataaagg gagtgaatca
     4561 atgatgagtc aaacaaaagt cgaacgttat ttagaagaac aaaagatcag ctatcaacca
     4621 cttgatttta gtcatgtggt agaagaatct ttcacagaag aactacaaaa aagagggatt
     4681 gatccagctt tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc
     4741 gcattgattc ctttgaacga tcacttagat tacaaaaaaa cgagacaagt gacgaaagat
     4801 cgtaaagttg gttttcctgg aatggatttt gtattaatgc atacaggata tccccacgga
     4861 gcaaacaccc ctattggaat aaaactcgca catcctgatt attgtttttt agcagacagt
     4921 tctttaaaag aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag aagccttcgg
     4981 ataaaagtag ccgatttaga aaagatcgtc catcctattt acgctgattt aattagttaa
     5041 gggacgagaa gagaagctaa gcagagcaga gacctcaaac ttgagattat atctgcttgg
     5101 tacttggtgc atcctgtatt atctgttaca attgctttgt acatactgta ctatcaatcc
     5161 aatgaaaata tatttagcag ctatattttt tcattgatgt gcaagtgatg aaatcgtttt
     5221 taataaaaaa gatacagaga aaacgcgagg tgtaaaatat gaaaattacg tatcatggac
     5281 attcctgttt aacaattgaa atggaaaatg gccaacgcgt gatgattgac ccgtttatta
     5341 caggaaatcc attgacagac ttaactgcag aagaagtaaa gacagattgg ttgctgatta
     5401 ctcatggtca tagtgaccac ataggagata tgattcctat tgcaaaagaa aatcaagcca
     5461 caattatcag tattgttgag attgctgatt atgcgaacag cagaggagta gattcattcg
     5521 gtatgaatat cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc gtacatgcac
     5581 agcatagttc aagctatgaa gtagacggta tcgttcagta tatgggcgag cctagcggaa
     5641 ttattattca agcagaagga aaaacgattt atcatgcagg ggatactgct tatttcagtg
     5701 atctaggatt gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg attggcgata
     5761 actatacgat ggggccagaa gatgcagcaa aggctgcgca aacaattcgt gccaagaaag
     5821 tagttccaat ccattacaat actttccctg tcattgaaca agatcccgaa tcttttgtag
     5881 agttactgga agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg
     5941 cagaaaatgg cgacaaaaca tgatttgatt ttagaatata ttgaaagttt acctgttggc
     6001 aatcgaattt ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac
     6061 cgtgcaatca aagatgcaga aaatgtcgga ctagtttcaa caattcaacg agtagggacc
     6121 atccgtatcg aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc
     6181 aggatcattg aaggggacgt tttaggcggg gcggcaggat tagacaaggt cttgaataaa
     6241 ttcgtcatcg gcgcgatgac taaacatgcc atggaacgct atattacgcc aggatcacta
     6301 atgatcgtcg gcaaccgggc ggaagtccaa aaattagcat tggaagatgg cgccgcagta
     6361 ttgatcacag gaggattcaa tacgactgct gagattgcaa agctagctga tgatcttgaa
     6421 atgccgatct tacgcacctc ttatgataca tttacagtcg caaccatgat caaccgagcg
     6481 ttaagcgacc aattgatcaa aaaagatatc atgcttgtca gtgatatcta tatgccagct
     6541 gaaaagactc attatttgca gcagacagat acgatttctg attatcagcg tttgtcagaa
     6601 gaaacgcagc attcgcgttt tccagtcgtc aaccgccatc atcgtttgat ggggattgtt
     6661 acggcaaaag atgttttagg gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa
     6721 gaaccaatca gtgtaaagaa aacaatgagt attgcctctg ttagccatca aatgatctgg
     6781 gatgggctag aagtcatgcc ggtcgtttcc gatgacttga cattggaagg cttgattacc
     6841 cggcaagatg taatgaaagc gatgcagctt gtccagcgac aaccgcagat tgcagatacg
     6901 atttcggatc agatatctgg aaatatccat acgattgata ctgaccgtga aggaaatcgt
     6961 ttggaaaacc ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg cgttggaacc
     7021 atttcttttg gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg
     7081 aaccaaaaac gaaatatctt gattgaacaa gtgaacctac attatttgcg gctgatccaa
     7141 ttggaaagcg aattagacat tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa
     7201 ctggatatcg aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa
     7261 gtaatggaac gttcttagaa agcaggagtg aaatgacagt acagcaagaa atagtagata
     7321 caataaaaaa atacgataaa atcattatcc acaggcattt gcgccctgat cctgatgcat
     7381 taggatcaca agtaggatta gccgaactat tacgggaaag tttcccagaa aaagaaatac
     7441 gtcaagtagg tgaaacgatt gaagggcttc gctttttagc agaaatgcaa gaagttgaag
     7501 atgtcttcta ccaaggagcg ctagtcatcg tgactgatac agctaattca cccagaatca
     7561 gtgatcaaag atatcagttg gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg
     7621 acccttatgg agatcttgtg tgggtaaata caaaagcaag tagctgtagt gagatgattg
     7681 cagaatttgc tttgatgttt cctgaatctt taaagatgaa cgttaaggct gcacggttac
     7741 tttatgcagg aatcgtcgga gatacagggc gctttttgta tccctccacg acgtcccgta
     7801 cattagagat tgcttcgata ttgagaaatt atccatttga tgcaagtgct ttgaacagag
     7861 agattgaaca aatgccgatg aaagtcgcta aactttccgg ttatctttat caaaatatcc
     7921 aggtggatga aaatggtgca ggaagaataa tccttccgca agaaattctt gataaatatg
     7981 gaatcgatga ttcagagaca gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc
     8041 tggcttgggg gatttttgtt cagcagcctt cagggtatta ccgcgtacgt ttacgttcta
     8101 aggggccgat catcaatgaa ttggctaaac gccatcatgg aggaggccat ccattagcaa
     8161 gtggtgcgaa tgcaaaagat ttagcagaag tagaggaaat ctataaagag atccaagata
     8221 tatgccaatc gtataagagc agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt
     8281 gtagtaaaaa tcacaaatgt atcttttcaa gatcttcttt ttccgttaca atagtgctca
     8341 ctaaagattt taaaggagaa agaagatgac attaccaaat tgcccagaat gtggctcaga
     8401 gtacacatat gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa
     8461 agaagaacaa gaaactgtca tagaagacgg attagtggta ttagatgcaa atggaaaccg
     8521 tctgttagag ggcgatagcg tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg
     8581 tgccattaaa caaggaacaa aagtcaagaa catccgttta gtagaaggcg atcataatat
     8641 cgattgcaag gtagaaggat ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa
     8701 ttaaagaata ttcaaggtct tttcacattc ttttcctact actaatgata aaataaagta
     8761 aacgaaaagg agtgaaacga aaatgtatgg ttatggaatg tatggttttt tagatccgac
     8821 atttcttttg gtcattcttg gattagttat ttccggaatt gcgtcagcgt acgtcaatag
     8881 tactttccga aaatacgatc aagtgagaag caaaaacaat gtgactggta cacaagcagc
     8941 acaatatata ttacaaagtc aacagataaa taatgtcggt gttcaacaaa ttgccggaga
     9001 tctcacagat aactacaatt caggtaataa gatgttgagt ttgtctcaag caactgcaca
     9061 gtctacttca gttgctgcga taggtgtagc agctcatgaa tgcggacatg cggtacaaga
     9121 tgcttcgggt tatgtcccat tgaagattcg tgcagcactt gttccagcag ctaactttgg
     9181 ttcgatgatt tcgttcccct tgattatggt aggtgtattg cttagctgga actctacgtt
     9241 aatcaatatc gggattttcg ctttttcttt agcattactt ttccaattgg tgacattacc
     9301 agtcgaattt aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg gactactgac
     9361 ggaagaagaa gtaccgatgg caagacacgt attatttgca gcagcactga cttatgttgc
     9421 agcagcgctt tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg
     9481 aagagattaa aataaagaag ttgtgaaatc agcgctttga tctgagctaa taccgagagt
     9541 tagctgattg aataccattt atttggaata agggctcttt gtcaataagg actgatgggt
     9601 tgtacaaaat caaatctggg tcagaatgaa cctcattctg gctcag
//
LOCUS       DAZKAS010000104         9623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000104 DAZKAS010000000
VERSION     DAZKAS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.106"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..2107
                     /locus_tag="ACG8H7_002094"
     CDS             179..2107
                     /locus_tag="ACG8H7_002094"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8984167.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="ACG8H7_002095"
     CDS             2245..2700
                     /locus_tag="ACG8H7_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP8984168.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2780..2908
                     /locus_tag="ACG8H7_002096"
     CDS             2780..2908
                     /locus_tag="ACG8H7_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984169.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3170..3439
                     /locus_tag="ACG8H7_002097"
     CDS             3170..3439
                     /locus_tag="ACG8H7_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984170.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /gene="rpsT"
                     /locus_tag="ACG8H7_002098"
     CDS             3839..4093
                     /gene="rpsT"
                     /locus_tag="ACG8H7_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP8984171.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4182..5228)
                     /gene="holA"
                     /locus_tag="ACG8H7_002099"
     CDS             complement(4182..5228)
                     /gene="holA"
                     /locus_tag="ACG8H7_002099"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP8984172.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5266..7539)
                     /locus_tag="ACG8H7_002100"
     CDS             complement(5266..7539)
                     /locus_tag="ACG8H7_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFP8984173.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7532..8071)
                     /locus_tag="ACG8H7_002101"
     CDS             complement(7532..8071)
                     /locus_tag="ACG8H7_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFP8984174.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8207..8425)
                     /locus_tag="ACG8H7_002102"
     CDS             complement(8207..8425)
                     /locus_tag="ACG8H7_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP8984175.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8422..8667)
                     /locus_tag="ACG8H7_002103"
     CDS             complement(8422..8667)
                     /locus_tag="ACG8H7_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFP8984176.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8783..9511)
                     /locus_tag="ACG8H7_002104"
     CDS             complement(8783..9511)
                     /locus_tag="ACG8H7_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP8984177.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2978 a         1865 c         1671 g         3109 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa
     4141 aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac
     4201 tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg
     4261 atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat
     4321 ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca
     4381 atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat
     4441 tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg
     4501 tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata
     4561 ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga
     4621 ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg
     4681 gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga attctctaag
     4741 gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt
     4801 gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca
     4861 aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta
     4921 ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat
     4981 actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa
     5041 cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt
     5101 tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag
     5161 taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta
     5221 ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt
     5281 ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg
     5341 atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg
     5401 gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt caatatggga
     5461 aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc
     5521 tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa
     5581 aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc
     5641 ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc
     5701 atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa
     5761 tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga
     5821 taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat
     5881 tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat
     5941 cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat
     6001 cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa
     6061 agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc
     6121 aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc
     6181 gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga
     6241 agaaaaatcg attattcgct caaataagat taatccctct tctatcagca aaagtaaagt
     6301 ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt
     6361 gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg
     6421 ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa
     6481 ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc
     6541 acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa
     6601 taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag
     6661 caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag
     6721 tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata
     6781 cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc
     6841 actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa
     6901 agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga
     6961 tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt
     7021 ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt
     7081 ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc
     7141 ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag
     7201 ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc
     7261 acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga
     7321 atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc
     7381 gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata
     7441 accccaattg tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat
     7501 caaccgattt cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt
     7561 gaagttttga aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt
     7621 cgatcaacgc ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca
     7681 caccagcttg caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt
     7741 ctgaaccttc cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat
     7801 ggatcgttcg caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat
     7861 ctccactgac tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg
     7921 ctcctacttc taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa
     7981 agtactggtc ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg
     8041 tatcgcatgc ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg
     8101 attaaaaagt actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct
     8161 gacaaaagat cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa
     8221 aaattcttcg aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat
     8281 ggcgttttgc tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat
     8341 atttttgttt tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat
     8401 agtctttttt cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag
     8461 ttgtttatgc tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata
     8521 gctaagtaca tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc
     8581 cgtattcata atatgaatcc cagatatttt attttggaga tagacattca gcattggttt
     8641 ttttaatgtt tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt
     8701 cctgccaagc tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg
     8761 tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg
     8821 tcttttcacc gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt
     8881 cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa
     8941 gttctgatgg agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct
     9001 gttttccttc cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag
     9061 ggatatatag gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg
     9121 cttcttctaa taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa
     9181 aggaatagat accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac
     9241 gagcatcctc ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg
     9301 aagaaagtat ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata
     9361 ataaaaaacc acttgcgata caaaagataa gaactatcat agacatccaa atgatcaatt
     9421 tgggtgaagc tagtattttc ttgctgattt tttctatcat tgttcttttt tcaaagaatg
     9481 accagtaaaa atcattcatt actcctttca tttaaaattg aagaaacagt gagctctggc
     9541 aaaatcgtcc cttcaacatc taattacgtc aattttttct tttgttttta tttttatcgt
     9601 gaagattttt gacgaaaaag agg
//
LOCUS       DAZKAS010000105         9620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000105 DAZKAS010000000
VERSION     DAZKAS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.107"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8H7_002105"
     CDS             33..656
                     /locus_tag="ACG8H7_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984178.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8H7_002106"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8H7_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8984179.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8H7_002107"
     CDS             2152..3033
                     /locus_tag="ACG8H7_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8984180.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8H7_002108"
     CDS             3036..3806
                     /locus_tag="ACG8H7_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984181.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8H7_002109"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8H7_002109"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP8984182.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8H7_002110"
     CDS             5117..6280
                     /locus_tag="ACG8H7_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP8984183.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8H7_002111"
     CDS             6397..6807
                     /locus_tag="ACG8H7_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8984184.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8H7_002112"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8H7_002112"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP8984185.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..>9620
                     /locus_tag="ACG8H7_002113"
     CDS             9380..>9620
                     /locus_tag="ACG8H7_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984186.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIY"
BASE COUNT         3342 a         1632 c         2074 g         2572 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt
//
LOCUS       DAZKAS010000106         9564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000106 DAZKAS010000000
VERSION     DAZKAS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.108"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..2447
                     /gene="leuS"
                     /locus_tag="ACG8H7_002114"
     CDS             33..2447
                     /gene="leuS"
                     /locus_tag="ACG8H7_002114"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP8984187.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            2795..4348
                     /locus_tag="ACG8H7_002115"
     CDS             2795..4348
                     /locus_tag="ACG8H7_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFP8984188.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(4729..6513)
                     /locus_tag="ACG8H7_002116"
     CDS             complement(4729..6513)
                     /locus_tag="ACG8H7_002116"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984189.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(6528..8249)
                     /locus_tag="ACG8H7_002117"
     CDS             complement(6528..8249)
                     /locus_tag="ACG8H7_002117"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984190.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            8349..9110
                     /locus_tag="ACG8H7_002118"
     CDS             8349..9110
                     /locus_tag="ACG8H7_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFP8984191.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3100 a         1705 c         1921 g         2838 t
ORIGIN      
        1 caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa gaaattgaga
       61 aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat gatcctgaaa
      121 agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg ctacatgtag
      181 ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga agtcaaggat
      241 acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa caatatgcgc
      301 tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc
      361 aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca actgatccgg
      421 agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt ctagcttatg
      481 aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca aatgaagaag
      541 tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca atgcgccaat
      601 ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa ttagttgatt
      661 ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta ggagcaaata
      721 ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca cgaccagata
      781 ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt agagaaatta
      841 cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct aaaaaatcag
      901 atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt gcttatgcaa
      961 tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta ttatcttcat
     1021 acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt gaattcgctc
     1081 aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa aaagctgctt
     1141 acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac aaacaagaag
     1201 caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa gaagtcagct
     1261 atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca atcccaatca
     1321 tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca ttacggttgc
     1381 ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca aatatcgagg
     1441 agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa
     1501 tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct cataacaaaa
     1561 aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc tacattggtg
     1621 gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa ttcttatacg
     1681 atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa ggaatgatct
     1741 taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg
     1801 tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg gggccattag
     1861 atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg
     1921 tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact acattgaatg
     1981 atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa
     2041 atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa gcctataaag
     2101 tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca ccgattgctc
     2161 ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt tcttacgcac
     2221 catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt gttttccaag
     2281 taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa gatgagctag
     2341 aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa
     2401 aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg gctctttgtc
     2461 aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt cattctgtct
     2521 cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt gattttcatg
     2581 ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat ctctgtaatc
     2641 atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct gaaattgaga
     2701 gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg gattttgacg
     2761 cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat ttattatcga
     2821 agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga aaagaataat
     2881 gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag ctgtatcgtt
     2941 cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa aattaaataa
     3001 tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta ttgtttttct
     3061 tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca
     3121 aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact atctttattt
     3181 tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc agttattgct
     3241 gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa
     3301 cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc
     3361 tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc tcattctttt
     3421 agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc atataaatgc
     3481 tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga caaatgaagg
     3541 gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa atgatctcaa
     3601 acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta attgggtatt
     3661 tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa cggggctgat
     3721 tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct catggtttat
     3781 ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta aaacatggca
     3841 aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg tattttatgg
     3901 caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa ctttgtacat
     3961 ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa tgatcaatca
     4021 gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga cacgtgaaag
     4081 tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt cgattccgga
     4141 aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta ctcaaataag
     4201 cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg atgcagggaa
     4261 aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat tcgttcagca
     4321 actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga cgttcagcac
     4381 cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata gtgtaactcg
     4441 tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc tcgtatttta
     4501 caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg
     4561 attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta tccaaaaagt
     4621 atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa aaaagctatc
     4681 gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt acgctatctg
     4741 aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt cagaatgtgt
     4801 tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat tttcaatcgt
     4861 tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt ccattgcttg
     4921 ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta aaataacgac
     4981 aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc cttgagataa
     5041 cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc gaataaagtg
     5101 atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat ccattttccc
     5161 gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta aaaccatccc
     5221 aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt cgaattcgat
     5281 ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg tttttcctgc
     5341 accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat cgacttgatt
     5401 catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt
     5461 cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt catctaatat
     5521 ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca cataagaagc
     5581 ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta agaagccgat
     5641 cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga tgaaggcgag
     5701 ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag cacgtttaaa
     5761 agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag cttgctgatt
     5821 gaatgttttg accactagat taccagctaa gatttcttcc attcgattat ttaaaacacc
     5881 cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct tagttgtaac
     5941 aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt cgatataaaa
     6001 cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat tgatacctgt
     6061 caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga tgtcacctac
     6121 ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga tttcttttcg
     6181 caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt
     6241 gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca atgcttgttt
     6301 gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata agagatcatc
     6361 gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga ataaactgca
     6421 tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa aacgtatcaa
     6481 agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta tattccttgt
     6541 gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg agtaccttgt
     6601 ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat tgtactgagg
     6661 cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc tgctcttaat
     6721 gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata gatctctgct
     6781 ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga ataattagaa
     6841 ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag ttcttctaat
     6901 tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt tcccatctgc
     6961 aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata actgatgtgg
     7021 gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt tacttttcct
     7081 atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc agatccggtc
     7141 ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc ttctagaact
     7201 ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc atttgtctga
     7261 tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat ctctgcttgc
     7321 agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac caataccata
     7381 gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc agtcacgaca
     7441 ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat agaagcataa
     7501 acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg gttcactttg
     7561 atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc atatgattga
     7621 ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt gatggtgatc
     7681 cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat cagtcgataa
     7741 acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa tagagaataa
     7801 cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat catttggcaa
     7861 aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt caaaagagat
     7921 ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg gaaatatttc
     7981 tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact agaggcaact
     8041 gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt ttttataact
     8101 gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt tggtacacct
     8161 aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag tcctttgttt
     8221 ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg atacttttcg
     8281 attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt
     8341 gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt gatttgccaa
     8401 aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga caagaagaga
     8461 atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc gttttttata
     8521 gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca ttgacggagt
     8581 tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg gtgatgaaac
     8641 aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc gatacaaacg
     8701 aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg gagtttgaag
     8761 atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg atgagtatgc
     8821 cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa gtacatttat
     8881 ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga ttcttattga
     8941 aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa agaatgtatg
     9001 aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat acggatgaaa
     9061 acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg
     9121 tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt ctggttcgat
     9181 tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt tttttagagg
     9241 aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga tgtaattgtc
     9301 ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc atggtgaact
     9361 gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag atgagaatga
     9421 taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt acattattac
     9481 aatatgttaa taaggcaaaa ataaatagat ggattgaata aacatatttt ttaatcaaat
     9541 agtccctact gtgactttcc tcag
//
LOCUS       DAZKAS010000107         9537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000107 DAZKAS010000000
VERSION     DAZKAS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.109"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG8H7_002119"
     CDS             147..1016
                     /locus_tag="ACG8H7_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8984192.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG8H7_002120"
     CDS             1225..2250
                     /locus_tag="ACG8H7_002120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP8984193.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG8H7_002121"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG8H7_002121"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFP8984194.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG8H7_002122"
     CDS             3333..5036
                     /locus_tag="ACG8H7_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8984195.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG8H7_002123"
     CDS             5038..7071
                     /locus_tag="ACG8H7_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFP8984196.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG8H7_002124"
     CDS             7082..7522
                     /locus_tag="ACG8H7_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8984197.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG8H7_002125"
     CDS             complement(7722..8849)
                     /locus_tag="ACG8H7_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8984198.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG8H7_002126"
     CDS             complement(8852..9529)
                     /locus_tag="ACG8H7_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8984199.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
BASE COUNT         3240 a         1648 c         1952 g         2697 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggc
//
LOCUS       DAZKAS010000108         9357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000108 DAZKAS010000000
VERSION     DAZKAS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.110"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8H7_002127"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8H7_002127"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP8984200.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8H7_002128"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8H7_002128"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP8984201.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8H7_002129"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8H7_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP8984202.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8H7_002130"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8H7_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP8984203.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8H7_002131"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8H7_002131"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP8984204.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8H7_002132"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8H7_002132"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP8984205.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8H7_002133"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8H7_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFP8984206.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8H7_002134"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8H7_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFP8984207.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8H7_002135"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8H7_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFP8984208.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DAZKAS010000109         9343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000109 DAZKAS010000000
VERSION     DAZKAS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.111"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..293
                     /locus_tag="ACG8H7_002136"
     CDS             99..293
                     /locus_tag="ACG8H7_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984209.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="ACG8H7_002137"
     CDS             562..861
                     /locus_tag="ACG8H7_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984210.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="ACG8H7_002138"
     CDS             complement(958..1392)
                     /locus_tag="ACG8H7_002138"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8984211.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="ACG8H7_002139"
     CDS             complement(1624..2334)
                     /locus_tag="ACG8H7_002139"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP8984212.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="ACG8H7_002140"
     CDS             complement(2324..3184)
                     /locus_tag="ACG8H7_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP8984213.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="ACG8H7_002141"
     CDS             complement(3189..3827)
                     /locus_tag="ACG8H7_002141"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP8984214.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="ACG8H7_002142"
     CDS             complement(3842..4141)
                     /locus_tag="ACG8H7_002142"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP8984215.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="ACG8H7_002143"
     CDS             complement(4184..5668)
                     /locus_tag="ACG8H7_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP8984216.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="ACG8H7_002144"
     CDS             complement(5688..6149)
                     /locus_tag="ACG8H7_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8984217.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ACG8H7_002145"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="ACG8H7_002145"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP8984218.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="ACG8H7_002146"
     CDS             7452..8222
                     /locus_tag="ACG8H7_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP8984219.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="ACG8H7_002147"
     CDS             complement(8495..9322)
                     /locus_tag="ACG8H7_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984220.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2892 a         1920 c         1475 g         3056 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaa
//
LOCUS       DAZKAS010000110         9298 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000110 DAZKAS010000000
VERSION     DAZKAS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.112"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..333)
                     /locus_tag="ACG8H7_002148"
     CDS             complement(<1..333)
                     /locus_tag="ACG8H7_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFP8984221.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSF"
     gene            complement(323..2497)
                     /locus_tag="ACG8H7_002149"
     CDS             complement(323..2497)
                     /locus_tag="ACG8H7_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFP8984222.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2513..4855)
                     /locus_tag="ACG8H7_002150"
     CDS             complement(2513..4855)
                     /locus_tag="ACG8H7_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFP8984223.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4877..7420)
                     /locus_tag="ACG8H7_002151"
     CDS             complement(4877..7420)
                     /locus_tag="ACG8H7_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8984224.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7430..7828)
                     /locus_tag="ACG8H7_002152"
     CDS             complement(7430..7828)
                     /locus_tag="ACG8H7_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFP8984225.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7909..8193)
                     /locus_tag="ACG8H7_002153"
     CDS             complement(7909..8193)
                     /locus_tag="ACG8H7_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984226.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8217..9215)
                     /locus_tag="ACG8H7_002154"
     CDS             complement(8217..9215)
                     /locus_tag="ACG8H7_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8984227.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2644 a         1995 c         1456 g         3203 t
ORIGIN      
        1 aaaacttggg acacttttaa catggtaacg ttgtaaaaaa gtttgaagtt cttcttgtct
       61 tggttctttt cttgtttcaa ttcgatatac attcctagag aattttttgt cgtatctttc
      121 caagaaagat tcaacttctt ggcatttttt acaatcattt cttgctagat acacaaaaaa
      181 tgattgattc gttttttgaa gttctagtac ttcttctaaa ttactattga ttagttttcc
      241 atttgattct tcgttagttt ttatcagttt aaaacaacca aataagagga aactaacaat
      301 aagtatccta acaataactt tcttatttcg catattgttt ctccaaattt ggtttccgaa
      361 tatagacgac tcgcggtggt tcctggacgt cttcagctgt tcgatacacc acaaattcgc
      421 ctgttctcaa tgctcgaatg cgattcggat tgaatttaaa gcggtctacg tctcgtttcg
      481 tgcctctttc tcctgtccaa tcaacctttc ccatatcaga ataacctgct tcttgtgtcg
      541 tcatttccgt taattcaatg tcttcttctg tccccattaa atctgcccaa taatcaattt
      601 ctgtcgggtc atttgtcttc ccaataaaga acgtattcac attcccaatc aactgttttc
      661 gaaggacagg atcaattcta tcaatgtccg caagcgtttg aggggcatac gccgcatgca
      721 ccccagcgcc tcgtccctta ttgactaagt ctataaggtt atcatttaga taagaagctg
      781 gttcgtcaaa tattactagg actttcgagc gattggctgt ttctcctgct tgtttataca
      841 cttctgacgc aaagaaatta atatctccca caagcatttg agccaatgtc cgaataaact
      901 cactataaat caatccgttt aacgagatat aaacaatctt tccttcttta acaacttctt
      961 gaatatctaa ttctcttttt cctccagaag tatcaaagag ttcaccaagc tcactatcca
     1021 ataacatatt gatattggtc tgtaaattct cagaagtcgc atacaaataa aacgggttct
     1081 tcttatgttc gtaacgaaca aacaaccgtt taaacaacgt ttgctcgatt tctgtcatga
     1141 gagaaatgtt ttcttttaat aatagataat acgtttctaa gtcaacggtt ttacgatcat
     1201 gttcaacgat tcgctcttca cttaactgtt ccacatcggc aaaatcacgt tctgttttgt
     1261 ccatcgcttc attctttttc gtttttttct tcgtggtttt cttcttttct acgaccactt
     1321 tttctttaaa taaatctgga tttttcaact gaatacgatc cgcaaacaaa tgtaagacgt
     1381 ttcgtggcaa taaataatgt tgtaaccaaa ctagatttct ttcaatttta tggctctttt
     1441 ggaactcgtc taaaagctga atcgttacct gtaataacgc tttggctgct gtcgaataat
     1501 acacgctttc tgtttctgct agattggtta atttatctcg aatggcaata tcattgccat
     1561 tttttacagg attataacgc aaatcgccac cgtccacaaa gacttcaatc gggcgattgt
     1621 atgcctctgc cacttctttt aacgcttgta aagtctctcc tgctcctttc ccatcgacta
     1681 agataacggg gatacgaaac ttacacgcat gttctgctag taattggatc accgtcgttt
     1741 ttcctgaacc tgtagtcgct ggaataatca catgttggtt caattcacta aagttcatca
     1801 cataagggag cccatactca tctgttccta atagaatcac tttggctaat gaagccagtt
     1861 gtttattccg caaagcaacg tcttttatga caattgctac ctggtacgct tgctgatact
     1921 ctgctgtata tttttgggac tcctgaaatt tattcttccg acgttccttg tattctttac
     1981 tatttagcaa agctgtttgc cgttctttct ttgaaacaac ttttcttttt tcaataaaag
     2041 acgcatacgc aatcaaacaa cttcctgcac ctgtcccacc taaaaggagg gcaaaaatcg
     2101 tttgtccatt aaactgcaac gcatgagaaa acaagtttaa tggaaataat aagtaacacg
     2161 acaaataccc tctaggaaga acaaaaataa aacaaaatag atttaatagc gtaaaaccta
     2221 acgcaaaaat aaaaaaaaga gagcttcttc gttttaagaa gctctcatac tcaaagtcat
     2281 aaatatgtgc acgtgtctct tttttatcta tcataaagac aatcgctaaa ggaatactgc
     2341 ctacaataaa aaatggatat agacaaacac ctaataaagt gacgataaaa taaagttgtt
     2401 gaaaccgact ttttggcgtt tctttttctt gttttctttt gcgatcgaca gaaaacattt
     2461 gctcaaaatg tgtcggtcgt tttggtgcat tcatcatact atcgatttcc tcctatcgtt
     2521 ttcgttgttt catttctctt cgataaatcg gttgtttttc tcgttttcgt ttcatttttg
     2581 cttgagttgc tcgaaactca ctccgtgtct tcggtgcttg ttttggtgat tttgtttgtt
     2641 tcatggttga gtatgcttta gatacggttg aaggcgcttt ggtttgtgct gttactcgat
     2701 tgactgctga catatgttcc cgaaccccct ctggacgtcg aacagatttc ggtgtggtag
     2761 aacctgttgc tcgtggtaca ttcccttttc ttagtggttt ttgtaccgca tgtttttgtc
     2821 ctgcaactgt ccgggttttc atcgaacttg tttttgttac gggagtaagt gcctccttcc
     2881 gacgacgact tgtttgtccc tgacgacgtt ttcgatcgtt caattctgtt ctaggcgtat
     2941 gcttctggcc ataagacttg tgtttttgct ttggattcgc taatgacacc accattgcag
     3001 aaccctgatt gcgatacgga ctacgagacg caccttttct acttctttct cttagactag
     3061 gattgccttg caattctcgt tgtaataaat cggctgtttt tcttgcctgt ttctccattg
     3121 ctgttcgttg catacgttct tgactttgct cttctgcttg tttcgcttga tcatgcaagt
     3181 ctcttgcttt ttgtgccata cgttccatac gatttggtgc tttatgacga ggttgttgtg
     3241 cggattcacg ccgaaaagca cttcgtgtgg cttgtttcgc cgtttgtggc atacgaacgt
     3301 ctgagccacc cccacgtttt cgttgacgtt cttgtctctt ccgtgcgttt tctttttctt
     3361 tttgtttctg tgcttttctc gcttctttgc gttctttcaa ccgttgtttg gcttcccttc
     3421 tcaattttgc ttctgcccca aatggatttc ccgcaaaagc taacgctcct cgaacaccca
     3481 ttcttccagt aaatagactg cctaaaaagc ctcggaacaa ataaattccc aacggcgcaa
     3541 acaaataaac aatgatcaag agaatttttt cccatggatc gtcttgtgta ctacttaatt
     3601 ggaacccgta actaagaaaa gaagaaaaaa ggatacatgc aaaacctgtc attcctttta
     3661 aaaacgtcaa aataccaaaa actttggcat agttaccaaa cacattcatt gtgacaagaa
     3721 acaatcctaa aaaaagtaag aacggcaaca tcggtactaa caatagttgc atagttccaa
     3781 taatcaatcg aaacatactt aagacaaaat aaacaacggt ttgaatcaga ttcacgacaa
     3841 aataaaacaa ggcaaatgct gtatttttcc aactattgta atattgtatg ttcttgtttt
     3901 tcagtgtatc tgcttcatac tctgtgactt tgtcatgaat tttctcccct atttttgtac
     3961 ctaaatcatt gtccaataaa agattgattc gatcatattc ttttccagca tatttgactt
     4021 tcttctcacg aattgtgtct acattggttg tgccataatt taacaacaga tatggttcat
     4081 aaacattact ataaaagacc cgagccgcca tcaatttgcc tgccgtttcc acttttttat
     4141 ccgctgttac ctcttctgtt tcttttgttt cataatttgt ttcttcttca ggtgttaaca
     4201 ctggattgac cttcacgaac gccgtttcca ctgtagaatc taaagaaaaa agaacatcaa
     4261 aaagattctt atttgtccca gaatctttta atacgcttaa cccagtaaaa ataaggatcg
     4321 tcataagaaa gacacggagt gcttgtttca ttcgagcctc tccagcatat ttgattgcca
     4381 tcataattgc cgaaaaactg attccaatag ttcctgcaat acttaacata ttgtccgcaa
     4441 ttttttgtgt taagttttga atgggttctc gaatggcgga aataatatct aattcaaaga
     4501 gaaattcaac cattgaagca ttgaattctc ctaacatttt tacgccactc caaacaaaat
     4561 ttttcatcac tcctgcaaca ttgtacgata gctttttttt aaggttcatt gactcatagt
     4621 cattggtcat caattcaaac gaattctttt tgtaattttt atacaattga tccacatcag
     4681 tcgttacttc ttttacttct actttatcgt tggcattctc tccttcagaa cgatcggttt
     4741 cagtgacacc cgcaccatca ttcatttgat cggcagaaac agtagcccct tgtagacaga
     4801 aaaaaagacc ccctaataaa aggagcgtcc accatttcag agattttttt cgcaaaaaaa
     4861 ccacctcttt tttgttttat cgttgttttt cattttctaa cattgcttga attttttcat
     4921 cttgattttc agtagaagaa acagccaata accaactatc aaacaacaca tcaatggcaa
     4981 tcggttgatt tcttcccaaa tgatcctgga ataaacaggt tcctgtttgt aattgttcca
     5041 tcatttctaa attggctgaa ttcacttcca tatcaaagaa ggacaatagt ttctctcgtg
     5101 cttctttttg tttcggacga aacgcaaatt tgtaagaaat taactctttt ttatcttctt
     5161 gatcgtaatc tttaaacgct tgcacaacaa ggtaaatatc cgtattattc gcacgtcctt
     5221 ttcgcaagct atcatcaatc aaatagcttc cttgtgcggt atcttcatac ccttttgcct
     5281 cgtcataaat aatcgcagcg tcttcttctt tgtttgtaga aaagatatac gtaaacttca
     5341 taatgacttc cataattgcc attcctgcaa gttgttcgct atttaatcga cccgaatcaa
     5401 tctctttttg tgtcggaatg cgtattcctt gtgtccctaa cacgttgatt tgtgaatcaa
     5461 aacgtatcgg cgtactgtgc tcattcccaa tgagaatctt tcctaaacca acgctatgcc
     5521 ctaaaatcaa tttagctagt tctttgtcac tttttcgaat ttcttcgata acttgactta
     5581 acgaatgctt acctggcatg gtgtaaacta catcgatggc gtcttggatc accgttttct
     5641 tgtggctcgc tgtttctcga ttggtatcta cttctcctaa cattcctaac acatttttag
     5701 cggtttgaat tgcctcttct tttggtaaaa acaatagagg atcaagcatc cctcgacagc
     5761 tttcatctga agataacgaa ataaaattaa ttcgttcata catagccaca aactctggta
     5821 tgtgtccgaa tttttcaagt gcctttaaaa agaacttttg ggtttcattt ttcggatcaa
     5881 tataaagaat tttttgacct aaaaacatca gccataaaaa aatatatttt acaaggaccg
     5941 attttccttt acctggtgga ccaataatca acgtatttcc attggtatgt tccgcgcctt
     6001 caatactttt ctttgttaaa ttgggataat accatactag tttactagat agcgctaacg
     6061 cttctttcgc agatttaaac ttttttgcaa taacaatccg tcctaaagga aatcctttct
     6121 tatttcctac ttctttcttt aagtccaagc cataatctgc cacaaaacct gtcgtagcga
     6181 cctgctcgta actacgaaaa cgttgcgtac ttccaattaa gcattgatta aacaacgtta
     6241 gctgatccgc taccgatcga taaaatttga aatctgtaga ctcaatcgca aattcaatat
     6301 ctttgactcg ttcttccaat tcttcttttg aagaagcaga tacaaccagc gtgacggtag
     6361 aataacacaa tcgtttttct ttgcgcttca gcttatcttt tacatccatt aaccgttctg
     6421 taccaaaatt taatacatcg tctgaatcta aaatgccgtc aacggtctca tattcttttt
     6481 cttgttcata aatccgtcca gccattttat taatttttcg ttcatcagat tttgaatgct
     6541 caaatcgaag tcgaatatgc gtatcaattg gaaaagataa gctatcctga agattttgaa
     6601 taaagctact cccatacatg gctaaaggag cgtccgtttg acttaaaaac gtcgaataat
     6661 gcgtttgatt caactgttca atcgcaatgt atcctgcttc attcgaaatg acgccctcgc
     6721 aaatttccat tggcgtaagc tgtctttcag gcaatttttc ttctgatcga tggaaaaaat
     6781 aataataaat ttgtccaatc tcttgggcag ataagcgctc tatcggctta taattggaca
     6841 attccgtata gagctccttt tctaatgttt catacgttgg taacacacta cttataggaa
     6901 ctcgttgtcc cgtaatacga tagataaact ttttaaaaac atgcttaagc aattgaaaat
     6961 attcgatcgg atcagacact ttcatttctg tagtgaaacg cacaaacaga tacgttcgat
     7021 actcgtttaa ttgcacttcg tcttttaaaa tctttcctgc ctgttcaaag tacactttcc
     7081 caagatctgc gcattctccc tttaccaaat ctcgaatggt ttcttcaatg tgcgtatcta
     7141 ggttaaattg acgtggaata ttgataaaat gatagtcata ttcatcatgt ccaagtatgc
     7201 cttcgccatc ttgaagatag ttcttgaaaa aatcaatgtc atttaaagga aatacttggg
     7261 aagaaagttg atagcctacc cacacatcat catttttggt caaaacaaaa tttcggataa
     7321 tggcgtctat cggaaattct aatcgaggaa gcttcgtttt tttctttttg aactgccaaa
     7381 tcccgataat aaagacgacc aaaagaaaca aaaacgccat ttactcgact cactccttcc
     7441 atttgtgtag ggaatacgtc actttttcat ggaaaaacgg cacatacatt tcatgttcaa
     7501 agcgttgttt ccgtttctga taataggacg ttcgcccaat taaatactcg aaaaagtgtt
     7561 tattgtccca tttcattgaa aacaatgtcc aaacgagaac accaatccca aaaatcataa
     7621 tcaaccagga ctttcgaaac agtgtctgca agaaactaat ttgattcaca tacgcaatca
     7681 aaaataggat caaaagaatg aagagaaata ccccacctat gactaattgt tccatgcgaa
     7741 tgccattaga aatccaggga attttttgtc ctttaatccc gtaaattttg tgagggtgct
     7801 taaactcctt tgtatagttg tattccatgt tcactctacc tccttttaca aaaaaagaag
     7861 cgaccagaag ttctaatctt ctgatcgctt ttgcagtttg tctttttctt atgataattg
     7921 tatccagggc acaatcattt gcgttacccc cacgttctct tcactatcct taataaagat
     7981 aatgttcttt agccattcac caatatcaac ggcattcgat aacaataaga cgagcattat
     8041 caaaataaag acgccaataa tggcaaacgc tgcccgcgca aagccttggg tcgcataagc
     8101 accaaccatt agaacaatcg caacgattgc caatagccaa atgacttgtc cagatacatc
     8161 atcaaataac gatttaagat caaaaagttt catttccatt ctcctctaag ttcattttat
     8221 tcatttaacg tagtaacaaa ataactatcg ccacttgttt ttacttccat tgaaaagttt
     8281 gattggaact cgtttccttt ctcaaaatag aaacgatact tcccttgcaa aaagtagttt
     8341 ccttctttat tttttactgc atgatccagt tgtacattga tcaattcgcc gccttctaac
     8401 cctttttcgt tagaaatcaa tgccaatttt tcatcatttt tgacatataa ctcaaaaaac
     8461 cgtttcacaa atgcttccag cttgatttct tcgctttcag tgaccgcttg cccagaagaa
     8521 taaaatcgat tttcctgata gttcacagaa ttggtattct tactctcaga aagattcaaa
     8581 aattgaacgc catcaagtac ttttaaaaca ttgttttgat aactgactgg aaaagaaagt
     8641 tgactgcttt tccaatgttc tccttctgta aaagtaacct cataaaatag gcgatacatt
     8701 gcttgtgtca aatcgatttc ttctagttta ataaagcgaa catctttcgc tactcgattg
     8761 gtagccgtcg ttacaaagcc tagatcagac gcttccaatc cttgtgctaa ataatgattg
     8821 acggaagcct cccattcgtt tcgtttttct ggcgaataag taaataacct ttcaataaaa
     8881 tgttgtccct cgtacttcaa ttgttccgta gaagacaagg tttggttgac ttcagttaac
     8941 aatgtttctg gattgacttg ttttttcact gctaaacgcg ttaatgtatc cactcgtcca
     9001 aagtttagga ggagcacgac acccattaac agaaacaaac aaaaaaaagc gcctgaaaat
     9061 acactcatgg cacttttttg cgaaaattgg atcattcgat ctgacggttc tgttttcttt
     9121 ttttggaaca aaaaacgttt cttttgtttc ttttccttct gttgtttcgg tggcttagtt
     9181 tggaacgctt gtggattaaa ttttgcatta ctcatgagaa atgccatacc gttctaacaa
     9241 taatttcggt attttgtcca caatcactac ttcgtggaca accacacttg ccccctcc
//
LOCUS       DAZKAS010000111         9245 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000111 DAZKAS010000000
VERSION     DAZKAS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.113"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="ACG8H7_002155"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="ACG8H7_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFP8984228.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1552..2973)
                     /gene="tyrP"
                     /locus_tag="ACG8H7_002156"
     CDS             complement(1552..2973)
                     /gene="tyrP"
                     /locus_tag="ACG8H7_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFP8984229.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="ACG8H7_002157"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="ACG8H7_002157"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFP8984230.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /gene="tyrS"
                     /locus_tag="ACG8H7_002158"
     CDS             complement(5384..6640)
                     /gene="tyrS"
                     /locus_tag="ACG8H7_002158"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP8984231.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6752..6957)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7220..8521
                     /locus_tag="ACG8H7_002159"
     CDS             7220..8521
                     /locus_tag="ACG8H7_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP8984232.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8590..9189)
                     /locus_tag="ACG8H7_002160"
     CDS             complement(8590..9189)
                     /locus_tag="ACG8H7_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8984233.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2789 a         1918 c         1639 g         2899 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag acgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagtcta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctac ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcagaatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
     6961 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
     7021 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
     7081 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
     7141 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
     7201 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
     7261 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
     7321 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
     7381 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
     7441 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
     7501 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
     7561 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
     7621 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
     7681 aaatgatttt gttttagtta atttaaatga tttgatccaa aaagacgaac aaggaaatct
     7741 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
     7801 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
     7861 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
     7921 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
     7981 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
     8041 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
     8101 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
     8161 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
     8221 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
     8281 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
     8341 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
     8401 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
     8461 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
     8521 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
     8581 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
     8641 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
     8701 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
     8761 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
     8821 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
     8881 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
     8941 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
     9001 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
     9061 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
     9121 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
     9181 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
     9241 tattc
//
LOCUS       DAZKAS010000112         9062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000112 DAZKAS010000000
VERSION     DAZKAS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.114"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..761)
                     /locus_tag="ACG8H7_002161"
     CDS             complement(93..761)
                     /locus_tag="ACG8H7_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8984234.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="ACG8H7_002162"
     CDS             895..1182
                     /locus_tag="ACG8H7_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984235.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="ACG8H7_002163"
     CDS             1598..3136
                     /locus_tag="ACG8H7_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFP8984236.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="ACG8H7_002164"
     CDS             3133..4098
                     /locus_tag="ACG8H7_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984237.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="ACG8H7_002165"
     CDS             4085..5407
                     /locus_tag="ACG8H7_002165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP8984238.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="ACG8H7_002166"
     CDS             5648..6391
                     /locus_tag="ACG8H7_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8984239.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="ACG8H7_002167"
     CDS             6406..7398
                     /locus_tag="ACG8H7_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8984240.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="ACG8H7_002168"
     CDS             7410..8147
                     /locus_tag="ACG8H7_002168"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984241.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="ACG8H7_002169"
     CDS             8137..8859
                     /locus_tag="ACG8H7_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFP8984242.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9062)
                     /locus_tag="ACG8H7_002170"
     CDS             complement(8943..>9062)
                     /locus_tag="ACG8H7_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFP8984243.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3015 a         1549 c         1890 g         2608 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 ta
//
LOCUS       DAZKAS010000113         8866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000113 DAZKAS010000000
VERSION     DAZKAS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.115"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..753
                     /locus_tag="ACG8H7_002171"
     CDS             106..753
                     /locus_tag="ACG8H7_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984244.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(846..3125)
                     /gene="helD"
                     /locus_tag="ACG8H7_002172"
     CDS             complement(846..3125)
                     /gene="helD"
                     /locus_tag="ACG8H7_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFP8984245.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3403..5226
                     /locus_tag="ACG8H7_002173"
     CDS             3403..5226
                     /locus_tag="ACG8H7_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8984246.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5273..5452
                     /locus_tag="ACG8H7_002174"
     CDS             5273..5452
                     /locus_tag="ACG8H7_002174"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFP8984247.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5789..7729
                     /gene="thrS"
                     /locus_tag="ACG8H7_002175"
     CDS             5789..7729
                     /gene="thrS"
                     /locus_tag="ACG8H7_002175"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP8984248.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7953..8801
                     /locus_tag="ACG8H7_002176"
     CDS             7953..8801
                     /locus_tag="ACG8H7_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8984249.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT         2796 a         1576 c         1882 g         2612 t
ORIGIN      
        1 tttttgtgtg atttattttc ttttctaatt caacctgtgg tatgatgaga gcataagtat
       61 catgtgaaat gatatgaagt atgtggaaga tggacgggga agaatatgga agcaaaaatc
      121 actttagaac cttttgaacg tatcttaagc ggttaccgca aagtagaaga gttagctgtg
      181 aacgtaactg attgttcaaa attagcgcaa aaatacgcaa gattcggcgt tgaaggctat
      241 cgattaggaa actatgtcgg tacagggtat ctgaaccgtt atttagagtg tatggtggac
      301 cgcgcaccaa tgctgattta tagacaaaat tatctgattc cgctgctttt tcgtcgttct
      361 gattcagcct ttcggttatt tgaagaagag tatcggatgg aggctttctt tctactatta
      421 gagtggagct tgaaacatcg tcctgaaaaa attcttatcg aaagaaatga gaagatagac
      481 acgaaaaaaa ataaagtgat cgatagtgca tacctagcat ttcgtgtatc agagattttg
      541 gattgtggcg gctatccaat cagcaatttc caatcaattg accaattcat tgaatggaat
      601 cgaatttacc gattgatcga caatggcggg attggccgtc atagcaaggt atttgaccca
      661 gaatatccag aaaacatgga agaactgaaa atgattattt cgcttgtcaa attaaaatat
      721 cctgaaacag atttggattt atatatagaa taaatgacct taaatgattg aattactttt
      781 tctgcaatta ttattgtaaa taaaaagaca actgctaaca ttaatgatag cagttgtctt
      841 tttacttaca gtactttttt ctcataagat gaaatatcca tattgtttaa caaaggactt
      901 tcattattgg aggtaaaaag ggtcaattca tgcatcgcac gtgtacacat ggtgtaaagg
      961 atcaatttgt cttgctgaga agaaaaatgt tctgcatcta catcccacaa gaaaactcgg
     1021 tcaaactcca gaccttttgc taaaaaggct ggaagtaaaa taatggaacg cttcataaag
     1081 tcactctcat ttaaaatcag ctggacttgt tgtttcaact cgtctggcag ttgagcataa
     1141 agagcttcgc attcttcttg tgttttagct atgattgctg tacgccaata ccgggctttt
     1201 ccctgagtcg ttcgaatggt atctgctaac caattcaatt tgatatcatc tgttgttcct
     1261 tggatgagca aaggaaggtc tccttttctt gctgttgttt ctacttgatg ttctttcaca
     1321 ataaaatgat tagcaaaatc agtgatttct tttgtggagc gatagcttgt cgttaattgg
     1381 aaacgagtga cttcacggtc cacgaaaagg tcttccaatg atccaacgat cgtttcattt
     1441 ccaaagacat tttgatttag atctccacag aaagtcaaat ttgcttttgg gaacaaggct
     1501 tgcagcaatg ccacttgtga tggtgcgaaa tcctgcattt catcaataaa gatatagcga
     1561 gctttgtgtg taaccgtcac tggatatgtt cggcgcatca gtaaaaagaa tagtacagca
     1621 tcctcttgac gcaattctct ttgacgcaag tgtgtacgaa cagttgtcaa atgttcctgc
     1681 cacctggtct cgctgatttt atgtcgtgtt agaagagaaa gtggcacagt ttgtagaaag
     1741 tggagatatt gtttcgttat attgacaaat tgataattca tgatccctcg atggattcga
     1801 cggaatcttt tacgtacgat ttggttggcc agttttttac gtaatgaacg ctccttttgt
     1861 tctgtatatt caagatttgg atctgttgcg tatagttctt ggagctgttc ttccgccaga
     1921 tctttgaccc attgctgacg tgtttcgtct ttttgcagac cgcctaattt tttcaggagc
     1981 ttcgtttgta atagagaaag tctatgatgt aaaggtaatt gttcatttgt ttctttatac
     2041 cattggcgaa tcgattcctt tgataggatc gtacggccat ttattttcat atcacggaac
     2101 aagggaccgt agtttgtgat gccttggata tagcgatcaa tctgatcgac taaggtcaat
     2161 ccgcttttca tcacttgtat cggatctttt tcccctagca aaaaaccact ttcttgttgc
     2221 tgttcatcta gtaaagaaaa ctctggcagc agctgcacca aataattttt gaatgtctgt
     2281 gtaggcacgc cgctttcacc taatgaaggc aatacagtcg agatgtaatc agaaaacaaa
     2341 tggttgggtg aaaatagaag aacgttttca gcctcgagcc acttgcgatt atgatacaac
     2401 agaaaagcaa tacgttgaag caaagcggat gtctttccac ttccggcgat tccttcaatc
     2461 agcattactt tacttgttgt gtcccgaatg attgcatttt gtgctttttg tatcgtagag
     2521 acgatgtttt tcatatggtt gcttgatgat tcatccaata tctctagcaa aaagtcatca
     2581 ttgattactt ccgacgtatc taccatagat aaaatacgtc cttcttggat tttaaattgt
     2641 cgtttcaact gcagatcgac atctattcta tctgtttcag tttcataaaa tgcgggacca
     2701 atctcacctt cataataaag attggcaatt ggtgcgcgcc agtcaataac gattgtttct
     2761 tctgccagat ctcttaaaga agcgatacct agatataatg tttctgtttc acggtcttct
     2821 ttaaagtcga tacgggcaaa ataaggacta tctttcatga cagacaacgt ttgcaggcgt
     2881 ttttgctgtg cttcaagagt atggtatttc aaaagcaatt cctgctcatg ttggcgatat
     2941 tcaacagcag attcataaaa agcttcattg gaaccagctc gaatcttgtg attagctgtg
     3001 tcttttgtcg aatgattcat ttcctgagtc aattcttgtt gttttgtatc cagcatattt
     3061 tgttcgatct ggattagttc gatcgtttca tccacgtgcg tttgttctag ttgtttctca
     3121 ttcatcgcat gtttgctcct tccgccgtaa ataacgaatt aaaagtatag catagttttt
     3181 cgaaaaaagg tactttttaa ctgatgaatc gaaaaagtaa tccttgctga atatttttgt
     3241 ttgtagaact gatgaatttt ggaaacgctg atagtgatag ttgttttcaa ttaaggccta
     3301 ttgttttaaa aagtgctagt caaggactat gcttaagatg aaagaggcag ctggaaattt
     3361 attactaacc tctctaagtc acataaaaga ggagaaaaaa caatgacaca tttcaaaaaa
     3421 tttttactta ctttgcttat tggtgggata gctttattaa gtgaatttgt ttttcaccag
     3481 ccggcccttg catttgcgat tgtcgcattt actggaggga ttctcgcctt cttgatgttt
     3541 ttggaaatga tcaagacgtt acgttcagga agatatggcg tagatgtatt agcgattacc
     3601 gcaatccttg caacattagc cgtcaaagaa tactgggcta gtttgatgat attgatcatg
     3661 cttacgggcg gagatagtct agaggattac gcgaataaac gagcgggtaa agaactgcag
     3721 tctttgttag ataacacccc aagaattgcc catcgattca aagaaggaaa attgcaggat
     3781 atagtaatcg atgaaataaa tattgatgac cagttactag tcaagcctgg cgaacttgta
     3841 cccgcagatg gtcgagtgac caaaggagaa tcttcggtcg atgaatcttc tttgacaggt
     3901 gagtcccgcc cggaaaccaa gaaacctgga gatcagatta tgtcagggtc tttaaatgga
     3961 gacggatcga tccaaatgat cgctgaaaaa cgtgccgcag acagccagta tcaaatgatt
     4021 attaaactag tcgaagaatc aaaagaaaag cctgcccgat ttgtccgttt agctgatcgt
     4081 tatgcagtcc ctttcacgtt gattgcttat ttgatcggtg gtatttcttg gtggttctca
     4141 aaagaccctt tacgatttgc tgaggtactg gtagtggcat caccttgtcc actgattcta
     4201 gctgcgccca ttgctttagt tgcaggaatg agccgttcaa gtaaaaacgg gatcgttgta
     4261 aaaactggaa caacgattga aaagctcgca cgtacaaaaa cgattgcttt tgataaaaca
     4321 ggaacgctta ccaaagggat attggaagta gaggcgattc agccagtcaa tggaataaaa
     4381 aaagaagagc tacttctgta tgcagctagt gcagaacagg aatctagcca tatcttagct
     4441 cgttcgttag tgaacaacgt gaaaaacagc caactgtatc ctgttagtca actgaaagaa
     4501 gtgaccggtc aaggaatcca agcagtagtc aataatcatg aagtaaagat tggacgtgcc
     4561 tcttttgttt cagctgaaga aatagaaacg tccaatacag caatctatgt aacaattgat
     4621 cgatcttttg caggaacgat cctattttcg gatcagattc gcccagaagc agcaaagaca
     4681 atcaaggagc taagaaaact tgatgtgaaa aatttgatga tgatcaccgg ggatggatct
     4741 gccattgctc aaacgattgc agaaaaagtc caattagatt ctgtacatgc ccgtagcttg
     4801 cctcaagata aactgaccat tttagaaagt atccctaaag atcaaagacc tgttgcgatg
     4861 gtaggagacg gcgtgaatga cgcaccagca cttgctgctg cagatatcgg tattgcaatg
     4921 ggggctcatg gttctacagc tgctagtgaa agtgcagatg cggtcatctt gaaagatgat
     4981 ctcttgcgtg tagcaaaagc agtagtgatt tcaagagata cgatgagaat agcgaaacag
     5041 tctgtactga ttggcatctt tatttgtatt tttttgatgt taattgcaag tacgggacta
     5101 atccccgcat tattcggagc tgttttgcaa gaagtcgtgg atacggtctc tattttgagt
     5161 gccttgagag caaaaaaaga tccagccatt tttcctgatt cagtacccaa gacaaacata
     5221 gtatgataga atcgagttga aaaaagaacg atcagaaaga ggagagagca ttatgccatt
     5281 cgtacatgtc gaattagtag aaggtcgcag tccggaacaa ttagaaaata tgatgaaaga
     5341 tatcacggaa gctgttcata aaaacacgca ggcaccaaaa gaacacatcc atgtcatcat
     5401 caatgagatg aaaaaaggaa catacggtgt aaatggggaa tggaaaaaat aagtttagtt
     5461 tcccacttga tttttctatt tattagtggt taaataaaca cataattgat tgatgagaaa
     5521 gccaactgaa tttgagagtc ttcgataaga gaggaaaagc taggctgaaa cttttccagg
     5581 agaaaaccaa aggatgacca ctttcgaaac ccttgttgaa gcaagggcgg tcggaccgtt
     5641 acaacgcttg aggaacgcat ttgcgtttga acattaggtg gaaccacgaa cttttcgtcc
     5701 tagtatctct tttcaagaga tgctaggatt tttttgtttt tcagcttgat gatctgatca
     5761 atcaaaacat ttcaaggagg aaactattat gataaaaatt acttttccag atggtgcagt
     5821 caaagaattt gagtctggaa caacaacatt ggcaattgct gaaagcattt caaaaagctt
     5881 agcaaaaaaa gctttagctg gtaaagtgaa cggaaaactc gttgatttga ctcgtccaat
     5941 cgaggaagat gcaagtattg agatcatcac accagaccat gaagatgcac ttggcttagt
     6001 ccgtcattca gcagctcatt tgatggccca agccatgcgt cgcctctatc caaatatcca
     6061 ctttggcgtt ggaccagcta tcgattcagg gttttactac gatacagata atggacaaaa
     6121 ccaagtaact gcagaagatt tgccggcaat cgaagcagaa atgatgaaaa tcgtcaaaga
     6181 aaatctgccg atcgaacgcc gtgttctttc aaaacaggaa gcattagaaa tttttgcaag
     6241 tgatccgtac aaagtagaac tgattagtga attgccagaa gaagaagtga ttactgctta
     6301 tcaacaaggc gaattcatcg atttgtgtcg tggaccacat gttccatcca ctggccgtat
     6361 acaagtattt aaattattat cagtggcagg tgcttattgg cgtggaaact caaacaatca
     6421 aatgatgcaa cgtgtatacg ggacagcctt ttttgacaag aaagctttga aagaatatat
     6481 ccgtctccgt gaagaagcaa aagaaagaga ccatcgtaaa ctaggaaaag aacttgactt
     6541 gttcatggtt tccccagaag taggttctgg tttgccattc tggcttccaa aaggggcaac
     6601 aatccgacgc acgatcgaac gttatatcgt agacaaagaa gttagtctag gttaccaaca
     6661 tgtatataca ccaataatgg gagatgttga gttgtacaaa acatctggac actgggatca
     6721 ttatcaagaa gacatgttcc cgccaatgga tatgggagat ggcgaaatgc ttgttcttcg
     6781 cccaatgaac tgccctcatc acatgatggt ctacaaaaac acgatccatt cttatcgtga
     6841 attaccaatt cgtatcgctg aattaggaat gatgcatcgc tatgaaaaat caggtgcttt
     6901 gtcaggttta caacgtgtac gagaaatgac gttgaatgat ggtcatacat ttgttcgtcc
     6961 tgatcaaatc aaagatgaat ttaaacgtac gcttgaattg atggtggcag tatatgctga
     7021 cttcaatatt acagattatc gtttccgctt gagctatcgc gatccaaaca atacagataa
     7081 atattttgat gatgacgcaa tgtgggaaaa agcccaagca atgctgaaag ccgctatgga
     7141 tgaattagaa ctagattact ttgaagcaga aggcgaggca gccttttacg gaccaaaact
     7201 agatgttcaa gtcaaaacag ctcttggtac agaagaaaca ttatcaacta tacaattaga
     7261 cttcttattg ccagagcgct ttgatttgac atatgttgga gaagacgggg aaaatacgca
     7321 tcgtccagta gttatccacc gcggaattgt ttctacaatg gaacgttttg tcgcttattt
     7381 aacagaagta tacaaaggcg cttttccaac atggttggca cctatacaag caacaatcat
     7441 ccctgtttct gttgatgcac atggcgatta tgcttatgaa atcaaagaac gtttgcaaat
     7501 gaaggggttg cgtgtagaag ttgatgatcg taatgaaaaa atgggctata agatacgtgc
     7561 ctctcaaaca caaaagatcc cttaccaatt agttgttgga gacaaggaat tggaagatgc
     7621 aacagtgaac gtacgtcgtt atggaagtaa agaaacagct gtagaagatt tgaatatctt
     7681 tattgatgct atggaagcag aagtcaaaaa ttacagcaga gaaaactaat aggtaaattt
     7741 cctgtaaaaa agtagtagat agaaagcaat ccgaacaata gataacaggt attcctcaaa
     7801 gacacagatg ctcaatagaa tctgtgggat tgatgggata agatattctg ttgggcggat
     7861 tgtttttttg ttttttatcc agtatacgac cgtgaataag ggtctgatat ggtatactta
     7921 catagactat cattaaaagg gtggaggaac ttatgagcgt aattagtcgt attgaaagta
     7981 tgatggaaga ttattcttca gcagaaaaaa agttggccaa ctacattatc ggtaatattg
     8041 agaaggtccc tacaatgaca gctaatgaat tagcagaagc ttccgggttg agtgcgccga
     8101 cagttgtacg tttttccaaa aaaatcggtt ttcaaagtct gacggacttc aaaatcacga
     8161 tttcaacaga actacagact ggaatcgatg aaggttttag tgatatagaa gcaaatgagt
     8221 ccttttactc tatcaaaaac aaacttggta acaatgcaca agtagcgata aaagaaacag
     8281 tagatattct agaagaagaa acgatccaaa aagtcgtaga gtgtttggaa tcggcagaga
     8341 ctgttttttt atatggggtt ggcgcctctt ctttagtagt agaagatatt ctgcaaaaat
     8401 ggtctcgtgt cggtaagcca atcatttttg aaaaagatat ccacgtcttg ctcccgcaac
     8461 ttgtcagcaa tgagaaaaag aaagtgcttt ggcttgtttc aaactcaggt agaagtgcag
     8521 atgtagtcgc attagccgag ctggcgaaaa gcatgaatat cgaaatcatt gcgttgacgc
     8581 aatttggaaa taacccgctt agcaagatag ccgatgtact cgtacaaaca tctagaccaa
     8641 aagaaatcac gaatcgaagt gcggcaacta attcactttt ggcccaattt gctacgatcg
     8701 atatcatttt ttatttctat atggctaaaa acgaaaagct tagcgaaaaa gtgaaaaaaa
     8761 cacgagaagc aatccaagaa tattttatgg attcgaaata aaaaccttac tattttttat
     8821 ataagttttt tagaataaca aaacgtagtt ttccacaaat aaatga
//
LOCUS       DAZKAS010000114         8837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000114 DAZKAS010000000
VERSION     DAZKAS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.116"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(315..398)
                     /locus_tag="ACG8H7_002177"
     tRNA            complement(315..398)
                     /locus_tag="ACG8H7_002177"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="ACG8H7_002178"
     CDS             926..1426
                     /locus_tag="ACG8H7_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8984250.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="ACG8H7_002179"
     CDS             complement(1616..1960)
                     /locus_tag="ACG8H7_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFP8984251.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="ACG8H7_002180"
     CDS             complement(2114..3034)
                     /locus_tag="ACG8H7_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFP8984252.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="ACG8H7_002181"
     CDS             3221..3712
                     /locus_tag="ACG8H7_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFP8984253.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="ACG8H7_002182"
     CDS             complement(3736..6111)
                     /locus_tag="ACG8H7_002182"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP8984254.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="ACG8H7_002183"
     CDS             6437..7063
                     /locus_tag="ACG8H7_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP8984255.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="ACG8H7_002184"
     CDS             7086..7790
                     /locus_tag="ACG8H7_002184"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP8984256.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="ACG8H7_002185"
     CDS             7982..8815
                     /locus_tag="ACG8H7_002185"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP8984257.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2678 a         1682 c         1559 g         2918 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctctt
//
LOCUS       DAZKAS010000115         8675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000115 DAZKAS010000000
VERSION     DAZKAS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.117"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACG8H7_002186"
     CDS             <1..1101
                     /locus_tag="ACG8H7_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8984258.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1615)
                     /locus_tag="ACG8H7_002187"
     CDS             complement(1280..1615)
                     /locus_tag="ACG8H7_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP8984259.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1599..1862)
                     /locus_tag="ACG8H7_002188"
     CDS             complement(1599..1862)
                     /locus_tag="ACG8H7_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFP8984260.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="ACG8H7_002189"
     CDS             complement(2322..3125)
                     /locus_tag="ACG8H7_002189"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8984261.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="ACG8H7_002190"
     CDS             complement(3103..4227)
                     /locus_tag="ACG8H7_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984262.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="ACG8H7_002191"
     CDS             complement(4243..5058)
                     /locus_tag="ACG8H7_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8984263.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="ACG8H7_002192"
     CDS             complement(5048..5920)
                     /locus_tag="ACG8H7_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8984264.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="ACG8H7_002193"
     CDS             complement(6005..7294)
                     /locus_tag="ACG8H7_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8984265.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7438..7971)
                     /locus_tag="ACG8H7_002194"
     CDS             complement(7438..7971)
                     /locus_tag="ACG8H7_002194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984266.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8017..8160)
                     /locus_tag="ACG8H7_002195"
     CDS             complement(8017..8160)
                     /locus_tag="ACG8H7_002195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP8984267.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DAZKAS010000116         8572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000116 DAZKAS010000000
VERSION     DAZKAS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.118"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="ACG8H7_002196"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="ACG8H7_002196"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP8984268.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="ACG8H7_002197"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="ACG8H7_002197"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP8984269.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="ACG8H7_002198"
     CDS             complement(3070..4323)
                     /locus_tag="ACG8H7_002198"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP8984270.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="ACG8H7_002199"
     CDS             complement(4329..5252)
                     /locus_tag="ACG8H7_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP8984271.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="ACG8H7_002200"
     CDS             complement(5473..7965)
                     /locus_tag="ACG8H7_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFP8984272.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="ACG8H7_002201"
     CDS             complement(7972..8439)
                     /locus_tag="ACG8H7_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFP8984273.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAZKAS010000117         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000117 DAZKAS010000000
VERSION     DAZKAS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.119"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8H7_002202"
     CDS             complement(<1..149)
                     /locus_tag="ACG8H7_002202"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8984274.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8H7_002203"
     CDS             complement(593..1714)
                     /locus_tag="ACG8H7_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP8984275.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8H7_002204"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8H7_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFP8984276.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8H7_002205"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8H7_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFP8984277.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8H7_002206"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8H7_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8984278.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8H7_002207"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8H7_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFP8984279.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8H7_002208"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8H7_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP8984280.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8H7_002209"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8H7_002209"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984281.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKAS010000118         8319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000118 DAZKAS010000000
VERSION     DAZKAS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.120"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..979)
                     /locus_tag="ACG8H7_002210"
     CDS             complement(11..979)
                     /locus_tag="ACG8H7_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP8984282.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1211..2044
                     /locus_tag="ACG8H7_002211"
     CDS             1211..2044
                     /locus_tag="ACG8H7_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP8984283.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2078..2917
                     /locus_tag="ACG8H7_002212"
     CDS             2078..2917
                     /locus_tag="ACG8H7_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8984284.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            2943..3503
                     /locus_tag="ACG8H7_002213"
     CDS             2943..3503
                     /locus_tag="ACG8H7_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8984285.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3702..5423
                     /locus_tag="ACG8H7_002214"
     CDS             3702..5423
                     /locus_tag="ACG8H7_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFP8984286.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5588..6730
                     /locus_tag="ACG8H7_002215"
     CDS             5588..6730
                     /locus_tag="ACG8H7_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP8984287.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6746..7957
                     /gene="thiI"
                     /locus_tag="ACG8H7_002216"
     CDS             6746..7957
                     /gene="thiI"
                     /locus_tag="ACG8H7_002216"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP8984288.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2861 a         1441 c         1731 g         2286 t
ORIGIN      
        1 ataatgatgt ttagttttgg ataaacgaaa tcaattcttc acccgacaaa ccatagttta
       61 cagcaatcaa tagttttaaa cgtgcttttt gactattaat actgttacag aaaataatcc
      121 ccattttttc tagttctttt cccccgcctt cataatcata aacaggctct gcgattccat
      181 tgaaacaacg agaaacaagt actacaggga ttttttggac aagaagcttt tccaaagcag
      241 ctaaggcttg tggagggaga ttccccgcac ctaaagcttc gattaccaaa ccgtctactt
      301 tcgtatgggc gatggcttcc aataagtccc cttgcatccc agcatatgct ttaacgatcg
      361 ggatagtccc atctacagcg gaaatgtcca gtcgctttgt ttctaaaagt tcctgcaaga
      421 ataaaattcg attcttagtt actaagccga tgggacccaa tgtaggtgtt cggaaagtag
      481 caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga atggatctca tcgttcatga
      541 ccaccaaaac ccccttatct agcgcttctt cacaacttgc gacacgaatc gcactttcaa
      601 aattataaag gccgtcactt ccaagttcat tacttgaacg cattgcccca gtcaaaacaa
      661 ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc agtttcttct aatgtatctg
      721 taccgtgtgt gatcaccact ccggatatac ctgatgcata agcggacaag atacgttctt
      781 ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg aagattgaag atatcttcaa
      841 ctaataattc tacatgcttg gggattgtga tttctgcatc taataatggg tttgccacat
      901 caggactaac accgcccgtg agcatgtttt ctttcatggc gatcgttccg ccggtgtgta
      961 gtacaagtat ttttttcatt ttacagactt ccttacgaat aattttgcat agccgttaaa
     1021 tcttattgta tactatataa aagagttttg ctatcagtga agaataacga atagcctaac
     1081 ttatcataaa aaagctgacc ttattaaaat ggaaaagact caaccttgac ctcctaatac
     1141 aatggagtat ctggttgagt ttcttttaca agaaagcagc caaggaaatc aatcacgaag
     1201 gtggccggaa atgatcagaa tcggattaac atcgtttaaa gaacatgaca aattaactgg
     1261 taaaaagaat aacactcttt atgaatatgc agggtttcta cccttagtgg aattagatac
     1321 tgcctattat ggaattcccc gaaagtcctc agttgagaat tggataagtg aagtacctga
     1381 atcatttcgc tttgtagtca aagtctatca aggaatcagc ggtcaaggag actggaagcc
     1441 ttattatcag accgaagaag agatgattgc gcatttttta gatgttatgt cgccaatggt
     1501 agatgctgga aagatgtttt gctttttagt ccaattcccc tcatcattca aatgtacgaa
     1561 agaaaatgtc cagtacttgc gcaaacttag aaagtggttt gagggttatc ctgtttctat
     1621 agaattgcgg gattattctt ggtacagcga tgaatttctt gaacgtacaa gaaactttat
     1681 gtctagagcc gctttttctt tagccatcat cgatgagcca cagttgctga atacaacgat
     1741 tccttttgat ccatttgtca ccaatccgaa ctttgctctt tttcgattcc atggtaggaa
     1801 tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa aaaagaacgt tatatcgtta
     1861 ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa gtagttgatt ctaagacaaa
     1921 agaggtaggt gtgattttta ataataattc tggtggtgat gcggctgaaa atgcattagc
     1981 actgcaaaaa atattaaata tcaagccaga tggactcaat ccaacccaaa tggatctgtt
     2041 ttaagggctt attttattca agaaaggggc gaagtctatg atcaaagcta ttttttttga
     2101 tattgacgga acattggtca ccaatcgttc aaaagcgttg gaatcaacaa aacaagcaat
     2161 tgaatatgct cggaaaaacg gcattttatg cggtgtagca acggggcgca gcccagtaaa
     2221 gatcaaagaa atcattgatg aattggaatt agatatgtat gtcgtgtata atggtcaact
     2281 ggtttttact gcagatcgca cgattatcga ccaccctttt gaacaaaaag tattggaaca
     2341 tattgttgaa tttgcagatg agaaccatcg acaaatcgtt tttggtgcga gaaatcggtt
     2401 agatggatca acgacgatgc tattaggaca atcgattttt atcaagcgac tagtcagctt
     2461 tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc ctccaggttt ttagccctca
     2521 ccgacaaaag gatcgttatg aaacgttaga gatattaaaa gaaccaattt atcaatgtat
     2581 attgctcagt ccggagtcag aacagaaaaa gttagaacat cgctttcccg agtgtacgtt
     2641 tcagcgttca aatacgtata ctgtagatat catcccaaaa ggtggttcaa aattactagg
     2701 catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa gaaattatgg cattcggcga
     2761 tcattacaat gatatcgaga tgctgaaagg tgtaggaatt ggagtggcca tggggaatgc
     2821 tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa tcaaatgaag aagatgggat
     2881 atacgaagga ctgaaacact tcggtctgat ttattagtca atgaaagaag gaaaaaatca
     2941 gtatggaagc agagacacct tttcaaaaag tagaaacatt ccacaaaact tttgatccaa
     3001 gaagaccaga gatacccacg gcttttacag aaaatgaagc tcatacacgt gctgcgttca
     3061 aaatagaaga acttgttgaa tttgttgcag ctacttcacc taataaagaa caactgcatg
     3121 aagctgttat gctgctgcac gaagcattag ataaagcaga agaaaaaata cttggtaaaa
     3181 cacagtgggg aagtgatccg ctggttgaac aagtagatgc actgacagat atcctttact
     3241 ttacttatgg ttctttttca ttactaggaa tagtgcctga tcgaatattc gaaatcgtcc
     3301 atcaggcaaa tatggggaaa ttatttccag atggaaaacc tcattatgac cctgtaacaa
     3361 ataaagtgtt gaaacctgaa aactggcaga tggaatatgc tccagaacct aaaatcgcgg
     3421 cagaaatcca acgtcagatt caagaagcga ttgctaagaa gaaatccgct gatataggca
     3481 tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt ttgttatctt tatacgatgg
     3541 aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa aatagtatgc tataatctag
     3601 aatagatgtc ttttgcacga gagacgtatt atttggattt atgccaagca gaacgccatc
     3661 tgttgcataa aagatgcata ctgaaaacgg aggaaccata aatgggaaat aatgttatca
     3721 tcggaatcgt cattgcaatc attatagtcg ccattgtcgt ctttttggtt agttacttga
     3781 tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa acgcaaggaa gaattatttg
     3841 acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt gggcaaagcc
     3901 aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc tcgttcgctg
     3961 aactagaaag tcaaattttc gaagtggaaa acctcaatga aagttttcgg ttcttaaaag
     4021 gaaaaaaagc agttgttgaa gcagaagcta caatggacca aatggagaaa caagtgacgg
     4081 aaatccgtga aggtctaaag gaattgcggg aaacggaaga acgcaactca ctagctgttc
     4141 agcaggcact tgacgtctat gaagaactga aagaaaaagt cagagaagac gagcatgaat
     4201 acggaccagc tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa gaatttacgc
     4261 agtttgtgac tttaaacact tctggagacc ctgttgaagc ccgcgaggtt ttggataaag
     4321 cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg ctgtatgagg
     4381 agctccatac cactttccct gaacaacttg aagaaatcag tgatacttac gagaaattga
     4441 tggaagaaca atatgtattc ccagaagaag atcttgctga agatatcgca aaagtaagtc
     4501 gaagaattga aaattcatta gccaatcttg aaaaaacaga agttgaaacg gtcgaatttg
     4561 aaaaccgtga aacagctgat ttgattgatt cgttatatga tatcttagaa cgggaaatgg
     4621 aagctcagag atatgtaaaa actaaccaat caacgattgc agagtatatc aaacatacaa
     4681 caaagaataa ccgtcaatta ttgattgagc tggatcacac atctcaaagc tatacattga
     4741 accacaatga aattggccgt gtacgaggtt tccaaaccga agtggaagaa atggaacgtc
     4801 aaaatgaaca gatgattcca cagatccgtc aacacgaaat tccttattca gaagtgcgca
     4861 cgttctataa aacagtcttc aaagtcttag aagatatcga aaagcaacaa gtagagatcg
     4921 acgactcatt acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag atcgatacat
     4981 tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg cctggcttgc
     5041 cgaatgatta cctagaattc ttttttgttg ctactgaccg tattgaagaa ctaagtgttg
     5101 tcttgaataa aattcgagta aacatggaag aggtcaatcg tttagttgct ctatgtgaag
     5161 aagatctaga attattagat aagaagacac atgatttagt agatgcagca gcattaacgg
     5221 agcaaatgct ccaatatgca aatcgttatc gtcatacaca cgaagaagtg agagaagcaa
     5281 ttgataaaag ctactattta ttcaataaag aatatcacta tcaagaagca ttagacgaaa
     5341 tcggtacagc attagagcga gtagaacctg gtgctttcaa acgcatcgaa gatttttatt
     5401 ttaatcatcc agacttagta taaagcaaaa aagttagtaa accagcaaga gcaagacgga
     5461 ttattgtctt gttcttgtct ttttttgtat atttgaaaat tgaaacaagt gaaaatttca
     5521 cctgctcttg aaagcacctt ggacaaccgt tataatagaa aagaatttca aaaaagaggg
     5581 gtttcttatg atatattttg ataacagtgc aacaacccca atctttccgc aaagcttaga
     5641 tgcatatgta aaaaccagtc agcggattat tgggaatcca tcaagcttgc atgatttagg
     5701 caatcaagct aaccggttgt tgcagcaagc aagaaagcag atcgctgatt tgatccatgt
     5761 agaaccagaa gaaatctatt ttacttcagg cggaacagaa ggagataact gggtattaaa
     5821 aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg atcatttcag gagtcgaaca
     5881 tccggccgtc agcgagacag cagaacaatt aaaagaatta ggaatcgaag taagtatcgc
     5941 cccagtggat aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa taagaaaaga
     6001 gacagtatta gtctctgtta tggctgtgaa taatgaagta ggatcgattc agcccatcca
     6061 ggaaatcagt gatcttttgg atgattttcc taaaattcat ttccatgtag atgctgttca
     6121 ggcgattgga aaagtaccat tctcgcaatg gttgacagaa agagttgatt ttgcaacatt
     6181 ttcagctcat aaattccatg gcccacgggg aactggtttc atttattgga aaaagggtcg
     6241 tcgtttagca ccattactga caggtggagg gcaagaaaga aatcagcgaa gtgggacaga
     6301 aaatgttcca ggtattgtgg ctatggttaa agctttacgt ttatcattag agataaagga
     6361 acaaaaacct gagcagacac aacgcttaaa agtagctcta atcaatgcgt tgaagacgta
     6421 tgagaaagtg acgatttttt cagaaggaac tgactatgca ccgcatattt tatgttttgc
     6481 tctaaaagga attcgcggcg aagttctcgt ccatgcctta gaagaaaaac aaatatttgt
     6541 ttctactact agtgcctgct cctcacgtaa acatatggca agcagtacat tgcacgcaat
     6601 gcatgtacca aatgatctgg ctacatctgc tgttcgaatc agcttagatg aaaacaattc
     6661 gcttgcagaa gtcgagcaat ttatgattat tttcaagcag ctttataaga aattttctgc
     6721 aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa attatggtcc gttatgggga
     6781 actttcgaca aaaggaaaga atcgaaaaag ctttatcatg caactagcac aaaatgtcag
     6841 acaagctttg aaagactttc cagaaatcaa aatccatgct gatcgggacc ggatgcatct
     6901 tttattgaat ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa tatttgggat
     6961 ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt
     7021 tcaagacatc atgaaagaaa tttatactgg gaaagaaaca tttaaaatta tcgctaaaag
     7081 aagcgatcac caatttgaat tgacaagtaa tgaattgaat caaacattag gtaatgctgt
     7141 ttttgatatt ttcccgcata tccaagttca aatgcgtcaa ccagatattc cattacgtgt
     7201 agagattcgg cgtgatggcg cttatctatc gtatgaaacg atcaagggag ctggaggatt
     7261 gcccgtagga acaagcggaa aaggaatgct tatgctttct ggggggattg attctcctgt
     7321 agcagggtac tttgccatga agcggggagt ggaaattgaa gctgttcatt ttgctagtcc
     7381 gccgtataca agtgaacaag cactgcaaaa agcaaaagat ctaacagcca aactagcacc
     7441 ttatgtggga acgatccatt ttatcgaagt gccatttacc gagattcaag aagaaatcaa
     7501 aaagtcagtg ccacaaggat attggatgac aattactcgt cgaatgatgt tgcgtttgac
     7561 cgatgaaatt cgccgtattc gtcatggttt agtcattttg aatggtgaat cattaggtca
     7621 agtggcttca caaactttgc aaagtatggt ggctatcaat gaagtaacga atacgccaat
     7681 cattcggcct gtagcgacaa tggataaact tgaaatcatt gaagtagcag aacagatcga
     7741 tacatttaat ctttctatcc agccttttga agattgctgt acgatttttg ctcctccaca
     7801 gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt tagcaataga
     7861 tgaaatgatc caacgagcac tagctggctt gaagatggaa gagatacaag cagaatcgtc
     7921 taatgaaaaa actgaagaat tttcagaatt gctataagag gttatgaaaa tagtgttttg
     7981 accagagtat tagagaaaca aaacgaaaaa atagctccat gtattttacc gtttttgaac
     8041 taatgtcgaa gattagctga ttgaatacca tttatcaaga agttatggaa aagccggttc
     8101 ttttttacta actgaaaaat aaaattttat ttagagatca agctacagtt gataaatgat
     8161 ataaaaaaga gaatttccca tgaaatagcg aggttgtgac agaagtgggc agcttcaaga
     8221 aataagaagg aatttacgaa aattgctttt caaatttttg tgaaatttcg gcttattttt
     8281 cgaagaagtt acttctggaa caccgtgtat cagaggttg
//
LOCUS       DAZKAS010000119         8158 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000119 DAZKAS010000000
VERSION     DAZKAS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.121"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..1050
                     /locus_tag="ACG8H7_002217"
     CDS             322..1050
                     /locus_tag="ACG8H7_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8984289.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            1068..2264
                     /locus_tag="ACG8H7_002218"
     CDS             1068..2264
                     /locus_tag="ACG8H7_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8984290.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            2257..3252
                     /gene="lacD"
                     /locus_tag="ACG8H7_002219"
     CDS             2257..3252
                     /gene="lacD"
                     /locus_tag="ACG8H7_002219"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP8984291.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            3249..4190
                     /locus_tag="ACG8H7_002220"
     CDS             3249..4190
                     /locus_tag="ACG8H7_002220"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFP8984292.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            4366..4845
                     /locus_tag="ACG8H7_002221"
                     /pseudo
     CDS             4366..4845
                     /locus_tag="ACG8H7_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            4858..5748
                     /locus_tag="ACG8H7_002222"
     CDS             4858..5748
                     /locus_tag="ACG8H7_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP8984293.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            5735..6544
                     /locus_tag="ACG8H7_002223"
     CDS             5735..6544
                     /locus_tag="ACG8H7_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP8984294.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            6558..6959
                     /locus_tag="ACG8H7_002224"
     CDS             6558..6959
                     /locus_tag="ACG8H7_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8984295.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(7005..7955)
                     /gene="yidC"
                     /locus_tag="ACG8H7_002225"
     CDS             complement(7005..7955)
                     /gene="yidC"
                     /locus_tag="ACG8H7_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP8984296.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         2626 a         1331 c         1759 g         2442 t
ORIGIN      
        1 tttgtcatta ataagctatt tttagttgtt gtaaatgata ttggaagtaa taaaacgata
       61 gttgtaggaa tatcataggg attaatgtgg atcacgctgc tcgataagtt agaccatttt
      121 gacggatcgc ttatgtggtt cgtctttttt tctgttcttt tattggtaac tggtattttt
      181 gaggtatctt tgtctattca agatataaag ctttaaagag ctgttataat agcttttttg
      241 gtctaaaaaa cctttgacaa atgattaata attcactatt cttgtgttgt ggttataacc
      301 agtaaagaag aggtgatttt tatggagagc tcaaataaag gaaaatcatt gtattatcaa
      361 ctagttgatt tattgaagga tcgtatcgaa acacagatga ctccgcatga taagctgtta
      421 tctgaaagag aactttgtca acactatggg gtgagtcgta ccaccgtacg tctggcttta
      481 caggaattgg aaaatcaagg ttatatttac cgaagacatg gaaaaggtac ctatgtctct
      541 gatttgtctg ataagtcatc gaacttagct ggagcatata gctttacaga agaaatgcgc
      601 gaaatagggc gcgtccctaa gacggtcatt ttagaattta gacaaatgga agctaataag
      661 agaattgcta agaatttgaa catccattta ggtgaaactg tatttaaact taaaagattg
      721 cgtttggcag atgaagagcc attgatggtt gagcgttctt atctaccggc gaaactattt
      781 atgggacttg atttgccgtt acttgaaagt aaaccgttgt atgatgtgtt ttcacaggat
      841 tatgaacaac aagttcaatt agctgatgaa gagttttatg caagtatcgt gcaaaaaaaa
      901 gatgctgttg tgctacgaat cgctgatggc gctcccgtct tacatctgac gaggataacc
      961 tataacgtga aaaatgagat tatcgaatat acagatagtg tagcaagagc ggaccagttt
     1021 cattataaaa ttcggcatat ccgaaaatga ttgagaggga gaacaacatg tttgagctat
     1081 cgaagaacaa attaaaacaa ataggtgcag caatcacgac acaagaaata aaacaacaac
     1141 cagatctctg gaaagaaaca atcgatagat tgatgcaaag ccaagaaaat ttagctctat
     1201 tttttgatca aataaaaaaa gaagcaaacg gaagaagaat acgtgttatt tttacgggtg
     1261 caggaacgtc tcaatatatt ggggatacgc tcttacccta tctaaatgaa catggagaca
     1321 atcagttttt tatcttccac agttttggca cgacagatat tgtagcttca ccaaaagaat
     1381 ttttgtttcc aaatgaaccg actttattag tttcatttgc tcgcagtggc aacagtccag
     1441 aaagtgtagc ggctgtgcaa attgcaaatg aacaagttga aacattgttt catttgacga
     1501 ttacttgtgc taaagagggg atgttggcaa aaaacgcaca ggatgataaa cagaattatc
     1561 tgttattgat gccagagaga gcaaacgatg caggttttgc tatgacagga agttttacgt
     1621 gtatgttatt gagtgcttta tggatctttg atcaaacagc aatggagaaa aaagaaagat
     1681 atcttgaaca attagttaaa atcggtcaag atcttctttc aagagatcaa gaaattcaaa
     1741 agttattacc tgattttttt gaacggatcg cttatctggg ttcaggaagt ttggcaggtg
     1801 cgacaagaga agcacaatta aaaattttag aattaacagc aggtaaagtt gctacaattt
     1861 ttgattcttc gatggggttt cgtcatggac caaagtcttt tttaaatagc gagacactcg
     1921 ttgtaggttt tgtgagcaat caaccatata cgcgaaatta tgacttagat attttagagg
     1981 aaataaaaga agatcgggtc actgccaatg tcattgcgat tgcgcaaccg ggtactattg
     2041 atttctcagg tatgactgtg ctattggaag actcggtgag taaattgccc gatgcctatc
     2101 ttgcattagc gatgattatg actgcacaaa tcattgcact ctatacttca atcaaagtag
     2161 ctaataaacc agatacacca tctccaacag gcacggtcaa tcgagtagtc aagggcgtca
     2221 ttattcataa gtatgaaacc gaaggagaaa tgacagatga atgaagaaaa aaatagggca
     2281 atggagagac tatcaacaaa agatgggatt attagtgcat tagcgatcga tcagcgtggt
     2341 gctctaaaaa aaatgatcaa atcgttggga aaagagccaa gtgaagacca aatcagtgaa
     2401 ttcaaagaac tagtatcagc tgagctgaca ccctatgctt ctgctatttt attggatccg
     2461 gaatatggat tgccagcagc aaaagtcaga gcaaaagaga cggggttatt gttagcttat
     2521 gagaaaaccg gctatgacgc aactacgcct ggtcgtctac ctgatttatt agcggattgg
     2581 tcttctctca gattgaaaga acagcaagcg gatgcagtaa aattcttact ttactacgat
     2641 gttgatgaag cacccgaaat caatcatttg aagcatgtgt tcatcgaacg tttaggtagt
     2701 gagtgcgaag gagaaaatcg tcccttctat ttggagttag tttcttatga tgcacaaata
     2761 agcgatagtt cttctccaga gtatgcgaaa gtcaagcccc acaaagttat tgagatgatg
     2821 aaagaatttt ctgatccaaa atataaagtt gatgtgttga aagtagaagt accagtcaat
     2881 atgaattatg tgaatggcta ttccacaggt gaatttattc acagcaaaga agaggctgct
     2941 gccttcttta aggagcaaag tgaagcgact ccattaccat tcattttttt gagtgggggt
     3001 gtttcaacag aattatttca acaaacactg atttttgctc atgaatcggg ttccaccttc
     3061 aatggtgtat tgtgtggacg tgcgacatgg aaagatgggg tcaaagcata cgtcgaaaat
     3121 ggaaaagacg gagctattca ttggttaaga acaaatggga gagaaaacat cgagacttta
     3181 aataaagtaa taaatgaaac cgctacccct tggttcaata aaacaaaaag caaaaaggtg
     3241 aagatgaaat gattacgaca gttaccttga atccttccat cgatatcgcg tatacaattg
     3301 aagaattaaa cctcgacaaa gtcaatcgag ttgaaacagt caataaaaca gcgggaggaa
     3361 aaggagtgaa tgtggcgagg gttctaaatt tattaggaga agaggtgata gctactggtt
     3421 taataggtgg ccagcacggt gaatatctaa agaaccagct agatcagtta gggatcatgc
     3481 atcagtttgt tccaattaaa ggagagacaa gggattctat tgctatcctg catagttcga
     3541 gacaaacaga gcttttggaa cgaggcccct tcataacaaa gaaagaacaa cagacattta
     3601 ccgatcattt cgagcagctc ctcgaaatat cagatattat gacgatctca ggtagtttac
     3661 cccaaggatg ccctcttgat tactataagc aattggttgc tttagctgat aaaaaaggga
     3721 aagacatatt actagatact tctggtaaat ttttgcttga agtattggaa ggtaaggaga
     3781 agccactact tatcaagccc aatctggaag aattaggaga actgattggc caacgtatct
     3841 gttcttcgat tcaattagaa aaaattcagt ccttattagc tcatccaata tttgaaggag
     3901 tgccatgggt tgttgtttct ttaggagaga gaggtgctgt ggcaaaagtt gctcagtgtt
     3961 tttacgaagt cattattcct aaaatatcag ttgttaatcc tgttggttca ggtgacgcga
     4021 cccttgcagg atcggcccat gcgttatcaa aaaggcaatc aatcgaagag attctcgcat
     4081 tttctatggc ttgtggtatg gcaaacgcgc aacaagagaa aactggctgg atcgacccta
     4141 tgaaagttac gatgttgatg aaacacattc aagttaagaa aatcaaatga acttaaatat
     4201 aaaagaaagc gttttcttta gttgggaagg aatttgttct tagcttaaaa atgaaatata
     4261 agaaagaaaa aatgaaaacg ttatctttga aacagaaggg atatttgatg atgtcacagt
     4321 tttaattgaa caataaatag aaaaaataga taaggagaat gcatgatggc aattattggt
     4381 gtaagaatcg atggacgttt gatacatgga taagtagcaa atctttggac aacgaaactg
     4441 aatattagtc gaatcatggt cgtggatgat gaggtagcca caaacacaat tgaaaaaagt
     4501 ggattgaagt tagcgactcc agcaggggta aaattaagca ttttgccaat cgaaaaagca
     4561 gcaacaaata ttttagctgg caaatatgac tctcaacgag tattgatcgt agcaaaaaag
     4621 ccagatcgaa tcttatcttt agtagaaaaa ggagttccta tcaaagaagt aaatgtgggg
     4681 aatatgtcac aaacgaatga aacacggtcc attacaaaat caatcaatgt ggtagatgca
     4741 gatatcgaag tatttaaaaa attagatcag cttggtatac atttaattgc tcaaatggta
     4801 ccaaacgata aagcagaaga ctttatgaat cttttaatca aataggagga atgatccatg
     4861 gatattcaat ggtggcagat cttattatta acattatatg caggttatca aattttagat
     4921 gaattacaga tctattcttc aatgagtgcg ccagtgtttg cagggttata tgcaggttta
     4981 gtaatgggtg atttaaacgc aggtttgatt attggtggca gtatgcagtt gacagtttta
     5041 ggagtgggga cgtttggagg cgcatcaaaa atagatgcca actctgggac gattttagca
     5101 actgccttct cgatttctct tggaatgaat ccagaacaag cgattgctgc tattgcagtg
     5161 cctgtagcaa gcttaatgat ccaattggat attttagcta gattcgctaa cacatacttt
     5221 gcccatcgta ttgaccgtca cgtagaagac atgaattatc aagcaatcga acgcaatttc
     5281 ttaatgggag ctttaccttg gtcgctctct cgtatgattc ctgtattttt agctttagca
     5341 tttggtggtg gattagttga aaatgttgtt tctgttttaa acgggaatct aaaatggcta
     5401 ggcgatggac tttcagtagc aggagctgtt ttaccagctg ttgggtttgc cattcttctg
     5461 cgctatttac cagtaaaaaa acatctatct tatcttattt taggatttac gatcacggca
     5521 ttattagcaa cgttattctc taatgttcaa ttattaggaa caagtgtggc aagcgttgtt
     5581 aaagatcttc cggcaacttt caattcgctg ccgatgcttg ctgtagcatt gattggtcta
     5641 gcactagcaa tgatcagtta taagaatgat caaaacaaag accatcaacc attagctagt
     5701 gagccatcac attctgttga gggggaaatc gaagatgacg aaatctaatt acaaattaac
     5761 aaaagaagat tttaagcaaa tcaatcgaag aagtttgttc acttttcaat taggttggaa
     5821 ctatgagaga atgcagggtt ctggctattt atatactatc ttgccacaac taagaaaaat
     5881 atatggagac aatacaccag aattaaaaga gatgatgaaa acacatacgc aattttttaa
     5941 tacttctaat ttttttaata caatcattac tgggattgat cttgcaatgg aagaaaaaga
     6001 gggaattgct tcaaaggaaa ccgtatcggg gctgaaagct ggattgatgg gttcctttgc
     6061 agcgattgga gattccgtat ttgctgcttt gattccaacg atttttgggg ctttggcggc
     6121 gaatatggca actgatggaa atccgatagg tgtgtttata tggatttttg ctcagatcgt
     6181 tgtcatggtt tttcgttgga agcaacttga atttgcatac aaagaaggaa tttccttagt
     6241 tactactatg caatctcgct taactgcatt aacggacgca gccactttat taggggtatt
     6301 catggtaggg gcattagttg cgacaatgat caatgttaaa ttttcatggg caccaacgat
     6361 tggcgatgtg acattgaata tgcaaaacta tatcgatatg atcttaccta aaattttacc
     6421 tgctgggatc gtcggtggtg tttattggtt attaggaaag aaaaacatga ctcctactcg
     6481 agcgattttt atcgtattaa ttgtgtgtgt tgctttatct gcattgggtg tgatttcgaa
     6541 ataagggaga gcgaaaaatg agtagacaat tggtattgat tagtcacgga aaattttgtg
     6601 aggaattgaa aaaaagtaca gaaatgatca tggggcctca agaggatatc tacacgttac
     6661 ctttattacc agaagatggc actgaaacat acaaacaaaa gtttctcact gtcacagatt
     6721 cgttaaatga ttttgtggta ctatgcgatc ttttaggtgg tacacctgca aatatcattt
     6781 ctaaactaat aatggaagga caacagatta ctctttatgc tgggatgaat cttccgatgg
     6841 tcattgaatt tatcaactca caaatgattg gttcagagcc cgagtttgtt cgtgcaggat
     6901 gtgaaagcat tgtctctgtc aacgaattaa ttaatggaat agcagaagag gaagagtaac
     6961 aaaaaagatg agggcttagc tctcatcttt tttgtcctat gtttttattt ccgcttctgc
     7021 tgctttcctg cgttgcggcc tttgttgttt gtactgtttt ttggttgttt gatttgtttt
     7081 gttgcttgtt tgttgttagc tggctttgct gattctgtca cgtcttttct tggagtgacg
     7141 acttgttttg gcgggttttg tttcaactct tctgccactt gtgctttcaa acgtggtttc
     7201 atcataacgt ttgtgatgaa tgtttgcaga cagctgaaca caccaccgac tacccagtaa
     7261 agtgtaacac cggctggcgc actgatcgac atgaagacga tcatagcagg agagacgatt
     7321 agcatcgtac gcatcgtctt tttctgttct tcaggaatac cgatcgttga gatgtatcct
     7381 tgaagcagat aggcgacccc tgcaatgaca acaagtacca tacttggtga gcctaaatcg
     7441 atgccgtaga aagatgattc ccgaatacct tctgtataac gtgccgtaaa gtacaaggca
     7501 gagaagatcg gcatttggat caatagaggc agacagccga ttccgccggt catactcaat
     7561 ccgttttcgc ggtatacttg ctgcatttct gcttgtgctg ccatttgttc ttcacgtgtt
     7621 gttgcagctt tcaatttttg ttgggctgct tcaacttgtg gttttaacgc ttgcattttt
     7681 tcagattgga tcattgtttt tttcgattga ctaataccta gtggcaagat gatgatacgg
     7741 acgatcacag tcattgcaat aactgcccag ccataagacc aattgaaatt ctcaactaga
     7801 tatgtaatcg cttggcccat aggatggacg agtactcgat aaacgattcc cgaagtgtca
     7861 gggtttccgt ttgaatccat ccgcacacag ccagataaaa agagcaggat actgacaagt
     7921 ccggatccca ataaccagtt tttccattta ttcatagaat acttcctctt ctataatttt
     7981 tacacaaact taactatact aaaaaagcgc atggaattca atcaacttca tagaaatttc
     8041 atataaatat tcgttctttt acgtagcgaa aaattttgaa aaaaagacaa aaaatcaaga
     8101 aagaaatact tggaaagaaa agagtgattc caagtgtttc tttcttattt aaaaaact
//
LOCUS       DAZKAS010000120         8132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000120 DAZKAS010000000
VERSION     DAZKAS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.122"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..725)
                     /locus_tag="ACG8H7_002226"
     CDS             complement(<1..725)
                     /locus_tag="ACG8H7_002226"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8984297.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKC"
     gene            complement(736..2112)
                     /locus_tag="ACG8H7_002227"
     CDS             complement(736..2112)
                     /locus_tag="ACG8H7_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8984298.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(2241..2888)
                     /locus_tag="ACG8H7_002228"
     CDS             complement(2241..2888)
                     /locus_tag="ACG8H7_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP8984299.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(2972..4399)
                     /gene="uxaC"
                     /locus_tag="ACG8H7_002229"
     CDS             complement(2972..4399)
                     /gene="uxaC"
                     /locus_tag="ACG8H7_002229"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP8984300.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(4473..5552)
                     /gene="uxuA"
                     /locus_tag="ACG8H7_002230"
     CDS             complement(4473..5552)
                     /gene="uxuA"
                     /locus_tag="ACG8H7_002230"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP8984301.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(5555..7186)
                     /locus_tag="ACG8H7_002231"
     CDS             complement(5555..7186)
                     /locus_tag="ACG8H7_002231"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP8984302.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7509..>8132
                     /locus_tag="ACG8H7_002232"
     CDS             7509..>8132
                     /locus_tag="ACG8H7_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8984303.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQN"
BASE COUNT         2623 a         1622 c         1094 g         2793 t
ORIGIN      
        1 aaacatttaa ataaaatatc agttcttaag ttttcccatg attctttttc tttattaacc
       61 aaaataggat aaaagaaaac tttattctta tttgcagctt ctagatcacc aggagaatca
      121 ccaatcatta atatcttatc caaatcacaa ccattttcaa ttacacttcg aataatgtct
      181 tctttttttc ctttatcttg acaacacagt tcatcaacta aggataataa ttcatgtctt
      241 tcccattctt cttgaatagc ttccttatta gctgagctta caataattat cttcccatat
      301 tttttcagag cttttatagt atctctagca cttaaaaagg gtttatctaa tcctttagct
      361 tgactaatct gtcgattgac ttcttccgac cattttaaag cttttaataa atcctctgac
      421 tgagagtatt ctatttccct cttcaaagat ggattagaca attcttttgt tacagatacc
      481 cagtgcttta atcgattaat cgatggtaca tcattttttt ctaatgtttt aactaaacct
      541 aaaaatctgt ttattccacg agtctctgaa tataaattaa tattattcca atcctgtagg
      601 aatttttccc tatgatgaac atcaaaaatc tctgcagcaa ttggcccgaa aaagagattg
      661 tgtttataag tcattgtatc catgacacaa ccatctgaat caatacaaaa tatccaactg
      721 ttcatgtttt ttctcctagt tatgtttttg atttagcaaa ttataatttt ctaatacttt
      781 ttctttagat aatggatata cgaaaaataa aatcaatcct acaacaaaga aacaaactgc
      841 tggaatcaac gttgaagagt tatacagctt ttgtaaaact tctccacttt gtacttttgc
      901 taacgaatca taccctatta tagaaagagt ccaacctccg acaccgccag ctaaagcttg
      961 tccaattttt cgggcaaaag aatatactgc ataaatagtt ccatcttctc tttggccaga
     1021 tttcacttct tgatcatcga ttacatcagt aatattcgcc caaatgactg tattaaaaat
     1081 acctaacccc atatatccaa cagaagtaag aactaagaat acatacatat tactaggctt
     1141 tatcaaaaat aaaagtaaat atacaatagc agagaagaac atccctactg acgccaattc
     1201 ttttttaccg tattttttac tgaaaattaa acttacaggt gaaacaagta ccaacatcaa
     1261 aataggattg ataaagttca ttaaaactat tccctgtgca ttattataat aatctggaaa
     1321 tacataattg ttcatactca ttgttaaaag ctgagcaagc aacaaaaaaa tagctgcagc
     1381 tattatagaa agcaacgctc tatttttagc taatcctttt aaagatttca acaatgaaac
     1441 ttttaactgc gattcatcac ttttaacccg ttctacagtt aatttataac atattacgta
     1501 acaaataact gcacacactg aaaatactcc tgccacaatt gtaaaagttg atccaccacg
     1561 tacaatttga gctccagaac tatctcttgt ataaataaaa attggtgtta acacgccaat
     1621 gaacatacct gctaaggtag ctcctatact acgaaatgtt gataacgctg tcctttcttc
     1681 tgcaacagaa gtgatagctg atgccattga accgtaagga atattaatag atgtataaaa
     1741 aatacttccc catagtatat atgttacata catatatata actttaatac tcatagaagc
     1801 tgatgccatt gatgtttgat acattaaaaa acttgctaaa gcgactggac cactcatccg
     1861 taaaatccac ggtttaaatt tgccagtttt agatggtttt gatccatcca caatacgccc
     1921 cattgttatg tctgtaactg catcaataaa tcttgctact aaaaataatg ttcctacggt
     1981 agctccgctt atccccaata cttttgtata aaaaatcatt aaaaaagaac tagcaaaaat
     2041 gaaagtgaaa tcattcccaa aatctccaaa catatatcct attttgtctc ttagaccaaa
     2101 ttttctttcc atataatttg cgctccttcc tattgttaag cgttttcatt ctgttgaact
     2161 gccaacttac gtactatact ctgaagtata aagagttagt atttattttt tacattttat
     2221 agtattattt attttttctc ctaataattc tttatctcat aaaacttatc tatatattta
     2281 ctagctattc gagttacttc ttcaaaatca cctttttcaa caggagccaa caaattccca
     2341 cctacaccaa ctgcaacaac gcctgcttca aaccactctt ctaagttatc tagacttact
     2401 ccgccagtag gcattatatt tacatatggt aatggagctt gatacgtttt tacaacagat
     2461 ggaccaaaag cagaacctgg aaatagttta atgatttcta ctccagcggt aagtgcctcc
     2521 tttatctcag taattgtcat acaacctggt aaataaggaa tttgatataa attacaaatc
     2581 tctgctgttt gtcgatcaaa agatggactt acgatatatt ctgctccagc cataattgca
     2641 atacgagcag taaacgcatc taaaactgta ccggctccaa ttattacatc tttttgatca
     2701 gcatattttt tagcaatttc tttgattatt tcttcagcat tcggaactgt aaaagttagt
     2761 tcaatccctt taattccacc cgcagtaatt gcatttattg ctttaatggc ctcatcttta
     2821 ttttttccac gtacaaccgc aatgactcca gccgcttcta acttacttaa aatttctact
     2881 tttttcatat ctatctcctt cttttatgga tacatactct agctagttgt actaacttac
     2941 aataaataaa tttaagcttt tattttttgt attattttct actttcgatt tcaaaaaaat
     3001 taaaataatt atacgcatta cgataacaaa tattctccac tatctgacta agatacattt
     3061 catcatctgg aattcgacct gctttaaccc actcgcttag taattgacat aatactcgac
     3121 ggaaatactc atggcgagga tagcttagaa aacttcgaga atcagtcaac atacctgtaa
     3181 aattcgacaa caaactttgt tgcgccatcg tagtcaactg ttgacgcatt ccttcagctg
     3241 tatcattgaa ccaccaagca catcccaatt ggattttttg aatcataccc ccttggaagc
     3301 tttgcattgc tgttgctagt ggaagccaat cggttggatt taatgaatat aaaattgttt
     3361 ttggtaagca cccactcttt tcagcagtgt cgaacaatgc tacaacattt tgtgtcaaac
     3421 tagcttgatc ccccatcgaa tcaccaccga catctactcc aatttgttcc tttaatatac
     3481 ttgaattatt tcttaaagca tttaaatgca tttgcatcgt ccaaccatat cttgtatact
     3541 cagacatcaa atgtaattga atacctgtaa caaattgtgc aatttctgat aaatggaaat
     3601 ccgtttttcc tgctaaagct tttattaaca gattgtcgag tattttttca tctgctattt
     3661 cataaaaata agtattcaaa ccgtgatctg ctaatcgaca gccaatttga tgaaaataat
     3721 ccactcgttg gctcaatgcc tgcttcaacc cattaaagtt tttgattggt atacccgctg
     3781 cttgactaag ttttaaaata tagttcgaaa atcctgatga tttgatactc cagaccttat
     3841 ctggacggaa agtaggtaat accttgaaag aattatcctg tttagaaagc tcagtatgat
     3901 atcgcaagtc atctgtagga tcatccgttg tacaaatggc tttcacatcc atcatcttca
     3961 gcaattcttt tgggcgatag gaatccgttt ggattttttc gttggcacgt cgccaaattt
     4021 ctggtgcatt cttctcagaa attgtcaaat caatatcaaa gtaacgttgt aattccaaat
     4081 gagaccattc aaaaatagga ttacctggcg ccttctccac tgtttttaca aaagctaaaa
     4141 atttttcata atcattacct gcaccactga tcaactcttc ttctatccca ttagctcgca
     4201 tcaatcgcca tttataatga tctccagctc ctttatcatt taaccatacc tgagtcaaat
     4261 tcttgtattg cttattttct agcaaacatg ctgggtctaa atgacaatga taatcgatga
     4321 ttggttggtg ttctgcaaat ttatgaaaaa gtcttttagc tatttcattc tctaataaaa
     4381 agttcttatc taaaaacata tttaagcact cctttccttc ccttagtttt tagaagagaa
     4441 aatttttctt ctaaatagac tccattatat aattatttac tcgccccctt ttcaagcgct
     4501 tcccaaatac catttaaata aactgcacct agagctcggt catataaccc ataaccaggt
     4561 ctaccggttt ctccccaaat catccgacca tgatctggac gaatatagcc attaaatcca
     4621 ttatcgtaca ttgtttttaa tatcttatac atatctagag aaccatattc tgatttatgc
     4681 ggcgcttcat aaaaatcgag tccattatca cttaaatatt tgatattacg aacatgtgca
     4741 aaaggaatcc gatcccgttg acaaaattca gatagaattt catagacatc attatctggt
     4801 cgttctgcaa tacttccagt acataacgta ataccattag aaggagaatc cactacatta
     4861 cacaaccatt ctaaatcgtc acgattttta acaatacgag gtaatccgaa aatagaatat
     4921 ggtggatcat ctggatgaac tgccatttta atcccaactt cttcacatgt cgggataatt
     4981 gctttgagaa aatatgcaaa gttctctcgc aatttcgctt catcaacttc tttataggct
     5041 tctaataatt gcttgatttg tgataaccga tgaggttccc aacccggcaa ttcaaaatta
     5101 ttagtagaat tttctacttt tctaataatt tcctctggat cagtaggaat attttttgaa
     5161 atataggcta acgtagaaga cccatcatct aaatgataat ccaaatctga ttttacccaa
     5221 tcaaagactg gcataaaatt ataacagata acctcaatcc caaattctgc caaattgcga
     5281 atcgtctgtt tataattctc gatgtattga tctcgtgttg gcaaaccaat tttgatatca
     5341 tcatgaacat taacactctc aataactttc aaggtaaggc cattggcttc gacttcatct
     5401 tttaaagctt taattcgttc tctcggccag acctctccgg ctggtatatc cattaatgtt
     5461 ccaacaattc ctttcattgc tgggatctga cgaatttctt gtagtgagat tttttcttca
     5521 tttgccccat accatctaaa agtcatttcc atagttactt tcctccaact tctgttatat
     5581 atttattgat tgttttacga actgcttgag gacctactag catttcgcaa aagatttttt
     5641 caattttctt tcctatctca acttgatata aatctaaacc aaatatttcc ttattttcta
     5701 agatttcctg taccgcacaa tgaatctttg caggatcttg ttcacctaat ttcaatactg
     5761 agaatttttc ctgcaactct accaataaag gatcaggact tggcacaaaa ttttttcctt
     5821 gatcatcgac tgccaataaa taacgtaacc aagcagcaat agcgatagga ataaatgtta
     5881 gattttctgc atttccgtct ttttcgacat actttttaat tgtttcacca tagcgaatcg
     5941 ctactttttg agaagtatct gttgcaattc gttgcggtgt atctggaata tagggattag
     6001 gcaaacgttt attgattact tcatcaataa attgttttgg atcaataacg cctggacttt
     6061 caactactgg taagccttct acataaccta tccctttaat taagccaacc aaatcatcat
     6121 cttgcatttc tgcctgaatt gatttatacc ccaacaaaca acctgttatt gctaatgccg
     6181 tatggagcgg atttaaacat gtcgtaacct tcatgatatc tgctttatca actgtttctc
     6241 tatctgtcat aatcacacca gccgcttcca gtttaggtcg accatttgga aaactatctt
     6301 caatgactag ataatggact ttttcagtat tggtaaaagc agcgatattg gtatttttcg
     6361 cagtatgaat tatatctaag tcttcgaaac ccgccttcaa taaatccatt ttgacacttt
     6421 ccgaaggatt tggcgtaatt ctatcaatca tactccaagg aaaagtcact ttttctttat
     6481 cctttaaata agcaataaat ttttcagaaa cgtatccttt tttttgccaa gcttctgcaa
     6541 tcataaaaat gctatcttga aaacgttgac cattctgtga gaaattatca gtagaaacca
     6601 tcgcaatcgg taactctcca gcattaaacc tgctatacaa caaggatgtc acaatactca
     6661 tcgtatgttg aggattggta ggtccagatt ttatatctgc ttccactacc gaaaaaaatt
     6721 gtcctttaga atcttttaaa ctgtatcctt tttcagtaat agtaaacgtt gtaaactgta
     6781 atgtaggatc ttcaaaatat ttaaccactc tttgatagtc cgttatattc tttggattgc
     6841 aatataaact atcagctgtt actgctaaaa gtcttttttt gagttcacca tcttcaccca
     6901 taacaatctc caaaatatca ttgttgtagt catgataagc tttttctact acttcttcat
     6961 caaatgtctc acacacaact actccggatt ctaattcatt ttgatctgct aagcattgag
     7021 caatttctgc atgaaatcct cgataaagat ttcctccacc aaaatgaagc cacactggat
     7081 gtttctgacc ttcttgtttg accgcttcga cattaaaata cggtagttga acttgcattt
     7141 tgttcaatcc ttcgatagaa tcttgccagt ctttaatcaa aaccatattt acccctccat
     7201 actgatatgt tagtttccgt tacaattgta gtataaaatc gaatgtaatc gctgtcaata
     7261 agaaaaaact aaaaatattc ttttttctac catgtcagaa ttattgtgtc tttttttagt
     7321 aaaagcacac caatatatat tttttagaac taacttaaat taaagcttaa tttctctttg
     7381 atcttatttg agtatttttt gtttttttaa tagctagtat gttactttga aatttgatat
     7441 tatataacga gcatagaact aaaatatcaa attcaaaaat gaaattttga gagggattaa
     7501 atatgacaat taaaactagt ctccaaaatc aagcttatca atctattcgt cagcaaatta
     7561 tctacgctga cttagaacct ggaaaaaaag tatcagaaaa aaaattggaa gaaacgcttt
     7621 ctatcggtcg tactccaata cgtgaagcat tgattcaact ccgtcatcaa ggacttgttg
     7681 atacaattcc tcaaagtgga aattatatct cattaatcga cttagattta gcaaaaaatg
     7741 ctcgttttgt cagagaacat ttagaacgac aaattatgct agagtgttgt gccaagatga
     7801 ataatcaaaa gaaacaagtt ttagaaacta taattgcaga acaagaaaaa gctgttagtc
     7861 aaaagaatgc tcgtagtttt tttcataatg ataatttatt tcataaagct tgttttgaaa
     7921 ttgcagaacg ggaagaagta tggcaatggt tggaagataa taatacgcac ttggaacgat
     7981 ttcgttggtt acgagtaaaa acggaaggat taaaatggga aaaaattatg gaacaacatc
     8041 atcaactatt taaagccttg accatgcaag accctgaaga agctaacttc ttaacatcct
     8101 tgcatcttca tttaatgctt gatgaacaaa at
//
LOCUS       DAZKAS010000121         8067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000121 DAZKAS010000000
VERSION     DAZKAS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.123"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..530)
                     /locus_tag="ACG8H7_002233"
     CDS             complement(9..530)
                     /locus_tag="ACG8H7_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFP8984304.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="ACG8H7_002234"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="ACG8H7_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP8984305.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /gene="cdaA"
                     /locus_tag="ACG8H7_002235"
     CDS             4610..5497
                     /gene="cdaA"
                     /locus_tag="ACG8H7_002235"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFP8984306.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="ACG8H7_002236"
     CDS             5494..6663
                     /locus_tag="ACG8H7_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFP8984307.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /gene="glmM"
                     /locus_tag="ACG8H7_002237"
     CDS             6687..8042
                     /gene="glmM"
                     /locus_tag="ACG8H7_002237"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP8984308.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2595 a         1486 c         1829 g         2157 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaag
//
LOCUS       DAZKAS010000122         7995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000122 DAZKAS010000000
VERSION     DAZKAS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.124"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1140)
                     /locus_tag="ACG8H7_002238"
     CDS             complement(34..1140)
                     /locus_tag="ACG8H7_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFP8984309.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1140..2276)
                     /locus_tag="ACG8H7_002239"
     CDS             complement(1140..2276)
                     /locus_tag="ACG8H7_002239"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP8984310.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2308..3759)
                     /locus_tag="ACG8H7_002240"
     CDS             complement(2308..3759)
                     /locus_tag="ACG8H7_002240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFP8984311.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3803..4567)
                     /locus_tag="ACG8H7_002241"
     CDS             complement(3803..4567)
                     /locus_tag="ACG8H7_002241"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFP8984312.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4595..5293)
                     /locus_tag="ACG8H7_002242"
     CDS             complement(4595..5293)
                     /locus_tag="ACG8H7_002242"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFP8984313.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5305..6084)
                     /locus_tag="ACG8H7_002243"
     CDS             complement(5305..6084)
                     /locus_tag="ACG8H7_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFP8984314.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6100..7044)
                     /locus_tag="ACG8H7_002244"
     CDS             complement(6100..7044)
                     /locus_tag="ACG8H7_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFP8984315.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7090..7869)
                     /locus_tag="ACG8H7_002245"
     CDS             complement(7090..7869)
                     /locus_tag="ACG8H7_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984316.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2246 a         1687 c         1213 g         2849 t
ORIGIN      
        1 tttttatact tgaatgtttc tgtgtcatat tctttaacct ctttgtatta attcaaataa
       61 ctcataatat tttgattcta agattttgta tgctgtttga atattataat ctgcctttat
      121 taaaatcgaa cgttctgaat ctcgatgaga gatatctaca tttgcaatct ctctcgccca
      181 ttcttttgcc gattgattta aacttatgaa attggtctta tcactgaaag caacttctgt
      241 aggaactttc tccgaaaaga aacacggtaa atctgcaaat tgagcttcaa tacccactaa
      301 cggaacccct tcaaataatg atggcaacat taatacgtca aatgcttgat aaaatttttc
      361 tatatcattt ctatttccta aaaatttaat atcatcatct atgccacttt tctttgcata
      421 attttttatt tcttcttcca tatcaccagt tccaataatt aataatacgg aattattctt
      481 aatagcgtga aattctttaa aaatttcaat caaaaaacga tgatttttag aatatgaaat
      541 tcttccaata tgtcccatta caaacgtctg acttgaaatt cctaatttca ttcgtacatc
      601 atttctaata ctttcatcat aataaaattt tttagcatcc actgcatttg gaatcaaagt
      661 gaatttatca tttcgaaaca aaaaatcacc tgctactttt ccacatgaaa attcatgtgt
      721 caaaacagac gttaacttac atcgataata ttgctttaaa agatatttaa aatctctatc
      781 tatcgatgta ttatggctat gtgctatccg tatcggaatc ccagcttttt ttgcagcttt
      841 taaaggtaaa taactcatcg catcaatatg agaatgaaca atctcatatt ctggatgatc
      901 cttaaaaaat tgttccatcc atttaaaata tttaggataa ttttgtggat aaagtctcgg
      961 tgcataatag acctttcctc caagacttat tatttcctca tcataatcac ttttatatgg
     1021 acgatgtgtc aaaaaatcaa actgaatttt agttcgatct atattcctat aatagttcat
     1081 tagcatcgtc tccaaccctg cacgatgcat atcattcaca cactgtaata ttcgaatcat
     1141 tatatatttc ccctatacaa atttaatgtt tcttgtacga ttttatccca attatattta
     1201 ctacaaataa actcagtagc ttgattcttc aaaactttaa ccatttccga ttgattgcat
     1261 gcctcttcta atcttatctt taaatcactt acatccgatt tcttaaaaat catagccttg
     1321 tcttctacta cttccgtaca ctctgagata tttgaaacta tacaacagtt cccataactc
     1381 attgcttcta acaaacttaa tggcattcct tctaaatcac ttggaagtgt atagatatac
     1441 gcattactat ataattcttc taattcctgt ccttgtacaa atccagtaaa tataattcgc
     1501 tcatcacctt ttgctaattc ctttagttca tttgcaaatt catctgtatc agagcttcct
     1561 ccggctatta taagtttttt atctgtctga acatctttaa atgcctcaat tagataccga
     1621 attccttttt cgggaaccaa tcgccccaaa aacaaaatat aagaatcttt tgttaatcca
     1681 tacttttctg taatcaatcc tgctgatttt atttcaattt ttttaacgcc atttggtata
     1741 aaccttgttt ttcttccata tatatcttca aaataatttt ggacgctttc actaagtaca
     1801 attatttcat ctgcaaattt tacagcacac ttttccccta acatgatgta tgtactagca
     1861 agcttgttcc atttttctct ttgatgatct agaccatgaa ttgttgcaac acatcttttc
     1921 ccaaaaagtt ttggcaacca caacatagca catggtcctt ctgcatgaaa atgaacaaca
     1981 tcatattttc cgaatgctgc tctaattcca ccaaatactg aagaactcat tgctgcaatt
     2041 ccttttatat ttaatgtaaa aatagtcttt aattttacac ctttatattc tttattttta
     2101 tgctggtcaa attcctttcc gctaacatga tgaccacttc gattatagca agtcacttca
     2161 tgcccaagtg caaccattcg cgttgccaat tcttctacca ctatctcaat tccaccttct
     2221 cttgatggaa ttcttttatg acctagcata cagattttta atttttttct tgccatcacg
     2281 ttttactcct tctatagtat ctttatctta catggaaccg tttctatgaa gcacagtttt
     2341 gactgtccta aacaaaattc taaaatctaa tcccatactc caattcctga tatattctgc
     2401 atcaagtttt acaacctctt cgaagtctgt gatatcactt ctaccactca cctgccacat
     2461 tccagtaatc ccaggtttca tcgcaattct cgcacgatga tgaaaatcat acttctcata
     2521 ttctgacttc aagcaaggtc taaaccccac caggctcatt tttcccatta ggacattata
     2581 aaattgtgga aactcatcca atgatgtaga tcgaataaat tgtccaattc ctgtcttctt
     2641 tgttccatca ggaagtattt tattgccaat gattcttggg tcaaaatcca tcttaaacat
     2701 gagattatta ctcatcttat tttctttcat caactctgct ttgcgttctt ctgcatccat
     2761 atacatacta cggaacttat acatcttaaa tgtcttacca ttttgaccaa tacgttcttg
     2821 tgcaaagaaa atcggccctg gtgatttgat gtaaataact ggtgctacga agataaaaat
     2881 aacacctgtt gcaatacatc caataattcc agcaagaatg tcaatcgttc tctttataaa
     2941 tgcttgcctc atcgtcataa cattaatact agatgtaata acagtataat caccaactcg
     3001 gccaataaat tgttttccac gtgtactatc agacactttt gtaagattca tatgtacagc
     3061 aagtcccatt tccgaacatc tggcgattaa ttctttggga tatggaacac tttcgtcaac
     3121 attgatgaat acttcatcta cccaattctg taaaagatat tccgctgcat tatcagcatt
     3181 tgcaactact gggacacctc cgatggtagt tccatacata tctctgtcta ctacaatcac
     3241 accactaagt ttatacatgc cataattctt ctctttaaga ttacgaacta cattttttgc
     3301 aatcttttga gatgttacaa taactaacga tacatcttca ccgtttttca tgcggtgatg
     3361 caacaaccct ttccaaagta tacgaattat atatgttaat aatccataca ccacccccat
     3421 tataaataaa gaaactctgg aataatcatt tccatcctgc attgtaataa ggtataaact
     3481 gcacacaagt aacagcataa aagattgctt tatagaagct acagcctctt tataatatcc
     3541 tctttttaaa acatttttat atgcctcaaa taaaaatata atcgtaagat tctcaagttc
     3601 tataaaaatt gccatgtggc gataaatagg aatcacatac ggattccagt caccctgtcg
     3661 cattacatat gatatataaa aagcaagctg gacacaaaat aagtctaata aaataaaatc
     3721 aatatgttta gaccaaccac ttgagttttt tctatacatc tctcactctc tcctcatttc
     3781 ctttttatct acgaataaag aactaaaata aaccaaactt cttctttttt acctcttcat
     3841 agattggata attgatctca tccccgttga tcagatcctt tgcgacttgt ttcatctgta
     3901 ctactttttc cttaccaaac tccttggcaa tctgctcata gcactctttt aagtaaaagg
     3961 tgcgcttaga gaccccgtgt gcatcagaag cagccatctg cacgagatta tgtttgacca
     4021 ttgttttggc tgttttctgg atatctttgc caaatacacc aacataactt ggcgcagtaa
     4081 gttgggccaa acatcccata tctaggaacg gaagcaaatg atttgggtct tctcgaaaat
     4141 gcgcgttgcg ttctggatgg acgatcactg gtatctttcc tagttctaac aactcaaaaa
     4201 aaagccgttc tgcatataac ggcacttcca gtgtcggaaa ttcgatcaga agatatgtat
     4261 catctaaatc tgtaaataaa atttcatctc ggtggatgtc ttcgatgagc gttccggtaa
     4321 tacgtacttc ttgtccttcc aataacgtca aaggcaactg tcttttctct aattcggctt
     4381 gtaacgaagc gactagagaa atgacttgtg atttttcatt gctgtatttt ccattattgt
     4441 ggtgtggtgt gcataaaata tgcgtaatgc cttgctgaat cgctttttca gccattgcaa
     4501 tacttgcttc taagttttct gccccgtcat cgattcctgg taggatatgg caatgtagat
     4561 cgatcatttc tcttgtctcc tctcttctta ttccttaatt tccgtagtaa taaccagcat
     4621 ctttgctgtg ttctgctccg ttgtatacga cacctaagac tcgtgcttgt accatatcta
     4681 acagttcttt tgctttagtc aatgattctt ttcgtgacgt attttcccgt acgaccaata
     4741 aggtgccatc cgtttttgaa gacatgatct gtgcatctgt tacggcaacg actggcggca
     4801 tgtcgaagat cactacatca aatagttgtc ttgcttctgc taaaatctga tccattcttg
     4861 gcgaacttag tagttctgat ggatttggtg gtttaggtcc acttggcaga atcgacaaat
     4921 tgtcgactgg tgttcgttgg atctcatctg ctacacttcc tgatgaacta agaacagtac
     4981 ttaatccgct tgcgttgttc aacttgaacg ttttatagat gacgggctta cgtaagtctg
     5041 catcgactag cagcacccgt tgaccagatt tggcaaaaac aacggcaatg tttgctgcag
     5101 tggtcgattt cccttctcca ggtcctgatg aagtcacaac aatcgttttg atttgctgac
     5161 cagctgcgga ggcaaactgg atattcgtcc gaatcgtacg atattgttca gcgattggtg
     5221 aagagggatt ggtgagtgtg accaaactga ctgcatgagt ttgggctgct ttttgtttct
     5281 gtgttcgtgc cattttcttt cctcctagac tcttgagcgg cttctgcgtg atggcgctgt
     5341 tttatcgttt gtagcttttg gttgtgtctg agctggtcct tttttacgga taccagcatt
     5401 caattctttc gctgacattt ctggaactgt tcctaagatt gggaatccta atgtatctgt
     5461 cacatatttt tcatctttca ctgtccgatc gagtaattcc aataagaatg ccagacctac
     5521 acctaccatc attcctaaca ttaagccagt cattaaattc aatttattat ttggcgaaac
     5581 aggtgtcgta ttggcaactg cacctgaaat gatcgagatt ttatcaacat tcatcacatc
     5641 ttttgcgttc tcttggaaaa cttgtgaagt tgtgttagcg atctgttgag cagccactgc
     5701 attcgtactt attgcctgaa tcgagaacat ttgtgaattt tctgcctgat taactgagat
     5761 ggcatctttg atttgttcag cagtcatttt cacattgtct tctgtttcta aacgatcttt
     5821 gacttcattg agcactaaat cactgacgat catatcttta tacgtattga tcatttgcag
     5881 gttcgtattg acatcattga catttgcagt gtctgattgt ggcagtgtca cgatcaattg
     5941 tgcttgtgaa ctgtattttg gtgtcaagat gaaaaatgtt acgattcctg agatggccaa
     6001 gccagcaaac attgagatga tgattattac caagtgtttg cgtaatgttt caaaaatttc
     6061 tttcaagctg attgtctctt ccattttttc tccactttcc tatttattct ctagattcat
     6121 ttgatctttt aattcttttt tcacacgatc aagttcttga tcactgactt tttgatagga
     6181 gatattatct tgcatgaagc cttctccttg cagctgatct tgcttaatcg taccaaatgc
     6241 ctcccgataa tctaaagcaa tttttttcat ttcatcaaag ttcatgtctg ttttcatatt
     6301 cgactctaga gcattcaaaa cggattgata gttgctgaca ccgtttagac tcaatacttt
     6361 tttcgctacg ccttctacga tctttctttg gcgcccttgt ctaccgtaat caccatttgg
     6421 gtcttcataa cgcatacgag aataagataa tgcctgatcc ccgttcaatg taattttacc
     6481 gattggaaag tcatagccat cttgtgagaa agtgaggtca ttattgactt cgataccacc
     6541 aactgcatca acgagttcct ttagcccttt catattgata gagacatagt gatcgattgg
     6601 gatgtctaca tatttttgta cggtatccat cgccatggca gctccgccga atgcgtaagc
     6661 gtggttgatc ttgtcttttg tgtcacgacc tacgatgtcg acatacgtat cacgaggaat
     6721 gctgacgatc gttgtctgtt tgtcttcagg actgactgtg gcaaccatca tcgtatctga
     6781 tcttccttga tcgacccgac ctaaatctcc agtatcaatc cccatcaata gaacagagaa
     6841 agattcttgt ttgtccagat caactggttt cgttcgttct ccttgggttc gttccactga
     6901 ttcgtaagtt ttctcaatag aatgtgagag atccagatat aattttgctc ctactcctac
     6961 aattgctaat actaatacga taagaactgc taaaataatc agaaatattt ttttccctct
     7021 tgattttctt cttcgtcttg ccatagtttc ctccgtttgc taaccttgct tgaatcaacg
     7081 ttttaactgt caatagctta tattgctata gcccatttgc tcagcaacag tttgtgcttt
     7141 tgttgcgtat gctgcgctta ttttttcatt ccggatctgc tctatttgac tgagcaccgt
     7201 caaatattga tcgtaagtag cggtggcagt cacttgacca tcctttaaca tcgtatcgaa
     7261 taactgatct agttgcgtga cttgcgcgat ctgaatatca gcgaatccta gatattgcat
     7321 aacaaccgtt ttccaaggac tatctttaaa aaagctcata ttttcacgta cacgagcgac
     7381 atctgcactt gtcacctttt ctttgatgat gacatcgtct acagcttgct gccagtttga
     7441 tacattttta gtaaataaat cgttgacagc tgtctggatc ttcagttttg attctgcact
     7501 atctaactgt gtcacgattg ctttcttctc atcttggatt gcaacagctt ctttaggcaa
     7561 ttctgattct ttgatgtcgt aatcatctgc tgaaagtttg acagaggata gcttatttga
     7621 caaacttgtg attgtttctt ctgttatttc agggtttaga aaaatttttt cttttgtact
     7681 atacagcttg tctacttctt ttgataaaga agtcaaggtg tctttttgat tggtgatggc
     7741 agattctgca taattgatcc gttgctgatg ttgccgctga ttattgatat ggtatccaaa
     7801 caaacagatc gcaatagcac ctgcacctac cgccacagca gtcaatgact tgacgaatgg
     7861 cttttccata gattcactcc taagtattga tgatagataa attaagatat aattcactta
     7921 cataaaaatg acagcttttt atttaaaaaa acataattaa actaatatat tcacagaaat
     7981 atttgtaaac aagtt
//
LOCUS       DAZKAS010000123         7937 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000123 DAZKAS010000000
VERSION     DAZKAS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.125"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..718
                     /locus_tag="ACG8H7_002246"
     CDS             17..718
                     /locus_tag="ACG8H7_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFP8984317.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1327..2343)
                     /gene="tsaD"
                     /locus_tag="ACG8H7_002247"
     CDS             complement(1327..2343)
                     /gene="tsaD"
                     /locus_tag="ACG8H7_002247"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP8984318.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2340..2804)
                     /gene="rimI"
                     /locus_tag="ACG8H7_002248"
     CDS             complement(2340..2804)
                     /gene="rimI"
                     /locus_tag="ACG8H7_002248"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP8984319.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2811..3353)
                     /gene="rimI"
                     /locus_tag="ACG8H7_002249"
     CDS             complement(2811..3353)
                     /gene="rimI"
                     /locus_tag="ACG8H7_002249"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP8984320.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3337..4059)
                     /gene="tsaB"
                     /locus_tag="ACG8H7_002250"
     CDS             complement(3337..4059)
                     /gene="tsaB"
                     /locus_tag="ACG8H7_002250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP8984321.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4250..5230)
                     /locus_tag="ACG8H7_002251"
     CDS             complement(4250..5230)
                     /locus_tag="ACG8H7_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8984322.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5254..6738)
                     /gene="galT"
                     /locus_tag="ACG8H7_002252"
     CDS             complement(5254..6738)
                     /gene="galT"
                     /locus_tag="ACG8H7_002252"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFP8984323.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6750..7739)
                     /gene="galE"
                     /locus_tag="ACG8H7_002253"
     CDS             complement(6750..7739)
                     /gene="galE"
                     /locus_tag="ACG8H7_002253"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP8984324.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2228 a         1705 c         1404 g         2600 t
ORIGIN      
        1 aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata
       61 tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt
      121 tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt
      181 gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta
      241 ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga
      301 gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc
      361 ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat
      421 ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt
      481 tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga
      541 tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac
      601 gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta
      661 tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga gagaataacg
      721 cgaacaaaaa taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta
      781 aaacttctga aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt
      841 gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa
      901 gcgtcttgtc ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca
      961 gtgagggcaa tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca
     1021 ataatgatta aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac agccctattt
     1081 cgattaaacg gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg
     1141 aggagctgtt ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact
     1201 cttgattaaa cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa
     1261 catgagaagc cgtttttcct gggctgttct tattacttga tgcagggcag ctttttcaca
     1321 acctctttat tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt
     1381 tcttttttgc atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag
     1441 tcgaagaggg ggaatcacta gttctacctc agggagttta gctgccaaag catcctgcaa
     1501 tccttcacgc aacccttgat tggccgctac acctccagct ataatcagtt gcttgactgg
     1561 ataattttga caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa
     1621 gctagctgct aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa
     1681 attgataaat gcacttttta atccgctaaa actgaaatca taattatctt catggatcat
     1741 cgctcgcgga aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc
     1801 gcttgggtaa gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc
     1861 atctcttgtc tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt
     1921 atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc
     1981 tgcatagata tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc
     2041 aaaagcctta gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt
     2101 gacagcgact gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa
     2161 gcataaggtg atttgttcta cgtgatggcg gctagctact tcaggtacta cgccaccaaa
     2221 acgtttatga ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct
     2281 aacgaccgct acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt
     2341 catttcatcg tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg
     2401 cgataatatg atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt
     2461 gctgcttcat tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc
     2521 gtaatcagta attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca
     2581 gcaatatttg tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc
     2641 tggttatctt cagctaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac
     2701 tgcattttcg tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat
     2761 tctgccaagt acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt
     2821 tctaaaagtt ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg
     2881 atcgttgttg cttgaaaccc caatttccga taaagtcttt gtgcatcttg attactcata
     2941 cgcacttcta atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca
     3001 ataagcagcg aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata
     3061 tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag
     3121 atacctagat atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc
     3181 catggcaatt cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct
     3241 gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt cgactgtttt ttctgtataa
     3301 atcattttat cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc
     3361 gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt ttaaatagcg
     3421 aggaagaaaa gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt gagccacagt
     3481 tacaccactt ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc
     3541 ctttatttca gagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag
     3601 cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacagtttc
     3661 taatctattt tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa
     3721 catgggcaca ataatattat ccacaccgat gcaattagcc gcgatggttt tcagactaga
     3781 tactcctact agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat
     3841 acgaagccct gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg
     3901 acttatttga agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt
     3961 gcgtttgata tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc
     4021 caaagtttgg tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat
     4081 gacattctcg ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc
     4141 aaaactagta caataataca tgattatctg ctggcgggca aattttgccc cttcctgaaa
     4201 cacaaaaaag ctgcggtatc actccatttt cccacagctt ttccatcttt tatttcgttg
     4261 attctcgaat aatcaactct gtttccacaa taatctttct tgaaaccggt gctttggaat
     4321 tacttaattg aagaatcgtc tccactgcta gctctcccat ccattcggta taaactttta
     4381 cagtagaaag cgagggcgtt acatatttcg ctacactgac atcattgaag cccatcacag
     4441 aaatatcttc tggtaccact aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta
     4501 atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg attcgttttt aaatattgct
     4561 tcactgcttg atatcctgac tcgacagaaa aatctgcttc gataattgga ccttcgtcgt
     4621 agcctttttc tgccaaaact tgttgaaatg ccataattct gggatctttt agccgctgat
     4681 ggtttttctt cgtatattct gttccagcaa gcatagcgat atgttgcgct ttatgggaga
     4741 caaagtgttc aatgactttt tgaacacttg acttaaagtc gacaactaga gagtcgattc
     4801 cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta
     4861 ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga agatcactaa
     4921 tggaatccca cgtttctttc aataaagtaa cgtttgcttc ttctgccttt ttttcgattc
     4981 ctaggcgaat agataaataa taaagatctt caagctcttc cgtatcgtcg taccactgga
     5041 tcaggcgaag cgtttgctta gtatttgctg catttttacg atgtttcgta taatttaagc
     5101 gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga tcaggatcat
     5161 agttgagaac tcttgaaaca gtagcaggag aaactcctgc caactgcgca atgtctttaa
     5221 ttgttgccat ttgtctctcc tcctttttta cgtttataaa atcgcagtaa atcgttcaaa
     5281 cgctgcctgt cctttttcat ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa
     5341 aacctgacct acagattttt gtacgacatc ggtgaccgta tctttcgtca aatcaggatg
     5401 ttcttttttt aattgttctg cccacggacg atgatattca gccatctggt tttcttctcc
     5461 taataagaaa tgctctactt ctactagttc tggtttcaaa cgaggtggta aaacagcaag
     5521 ccccattact tcgatcaacc caatgttctc ctttttgata tgttggacat ctttatgtgg
     5581 atgaaaaatc ccatcaggat gttcttctga aacattgttg tctcgaagga caaggtccaa
     5641 ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc
     5701 tgctgaaaaa gctctgatag atactgtttc atccgaatat gctttccatt tttccaatac
     5761 tgagttagct gcttcaataa gcaagttttt ctctgttgat tgcaaacgga tcacagacat
     5821 cggccatttc acgactcctg cgatgacttg cggataatct tctagtttga taagtttttc
     5881 tatcggtgct tttgccatag gaaattcatg acgtcctgcc tgataatgat catgagaaag
     5941 aatcgatcct ccaacaattg gtaagtcagc attcgaacca acaaagtaat gaggcaagac
     6001 ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg
     6061 ttctgataaa atgatcgaat gttcattgta ataagcataa ggggaatatt ggaatcccca
     6121 gctttcaccg tctagattca ttcgaataat ccggtgattg gtgcgggctg gatagttcaa
     6181 gcgacctcta tatccttcgt tttccatgca aagcatgcag gtaggatagt ctacttttac
     6241 cgcctcgcgt tctgctgcga tttgttttgg atctttttct ggcttagata aattgattgt
     6301 gatttccagt tcaccatact ccgactctgc cggaaatttg atgttttttg cgatggctct
     6361 tgttttgatg tagtcatttt tcttacttaa attaaagaaa tactcagttg cttcctctgg
     6421 gcttttcgaa tagtgctgtg caaagaatgc attgacgact gaaggtggcg gtgtgagaaa
     6481 atccattaat tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa gaacaccatt
     6541 ttctcttgcc ttagcgacaa gctgatccaa taagtctaca gattcttcct taactggtct
     6601 tacttcaatg ctttctagag catcttctcc taccatccct aaaagacgat tctgtaaata
     6661 taagcggtcc atttccatcc atccgccagc ttcgatcgca agtgtggcaa aatcacaaat
     6721 cgtttgactg attgacatat ctcattcccc tattcttcat aaccatttgg atggctgaca
     6781 tgccaatccc atgctgtttt gataatcgct ttgatatctg tgtattcagg tttccagccc
     6841 agaatctctc tcgctttttc acttgaagcg actaatcggc taggatctcc agctcttctt
     6901 ggtgctattt ctgctggaat ttcttttcct gtcacttctc tagctgcttc tagcatttcc
     6961 ttgactgagt atcctttgct gctgcctaaa ttgaagaagt tgctttcatt tccttctttc
     7021 aagtattcca aagctaaaat atgtgcagca atcaaatctt ctacttgaac ataatctctg
     7081 atacatgttc catctggtgt atcataatcg tctccataaa cagccaaggc ttttctttga
     7141 ccaagagcta cttgtaaaat gattgggaca agatgcgttt ctggtgtatg atcttctccg
     7201 atagatgcat ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac atagcgcatg
     7261 ccatatgctt ggtcacacca cttcatcatt ttttccatca tcaatttact ttcaccatac
     7321 ggatttttag gattagctgg tgtatcttct gtgattggag actctttcgg ctctccataa
     7381 gttgctgcag tagaagagaa tacgatttta ttcacgttga actcgtgcat gacttcaagt
     7441 aagatttgca taccgtatac gttgttgttg aaatacatca atggtttttc gacagattct
     7501 cctactaaag agcttgcagc aaaatggatt actccttcaa tcggttcctt ttcaaatacg
     7561 gaacgtagaa attctttatc ccggatgtct ccttcataaa aatgagcatc tggatgtact
     7621 gcttgtttgt gacctgtgag caaattatct accacagcta cttgataccc cttttgaact
     7681 agctggtcta cagcgtgcga accgatataa ccggctccgc ctagtactaa aattgtcata
     7741 aaaaacactc ctttgattag taaaattgtg tttgttgatt ctatataatt taaaaaacga
     7801 agtttacttt tatggtgaaa acaaacaaag taaaatttcg taaattcata tggtaaattt
     7861 ataataaaac atttagtaaa cattttcaag ggttttttgc aaaggtttac aaacaaacgt
     7921 aaaactagcc ttttttt
//
LOCUS       DAZKAS010000124         7847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000124 DAZKAS010000000
VERSION     DAZKAS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.126"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1015
                     /locus_tag="ACG8H7_002254"
     CDS             152..1015
                     /locus_tag="ACG8H7_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFP8984325.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1144..2766)
                     /locus_tag="ACG8H7_002255"
     CDS             complement(1144..2766)
                     /locus_tag="ACG8H7_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFP8984326.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3015..3536
                     /locus_tag="ACG8H7_002256"
     CDS             3015..3536
                     /locus_tag="ACG8H7_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFP8984327.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3722..5452)
                     /gene="spxB"
                     /locus_tag="ACG8H7_002257"
     CDS             complement(3722..5452)
                     /gene="spxB"
                     /locus_tag="ACG8H7_002257"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFP8984328.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5617..6435
                     /locus_tag="ACG8H7_002258"
     CDS             5617..6435
                     /locus_tag="ACG8H7_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFP8984329.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6538..6735)
                     /locus_tag="ACG8H7_002259"
     CDS             complement(6538..6735)
                     /locus_tag="ACG8H7_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984330.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6904..7482
                     /locus_tag="ACG8H7_002260"
     CDS             6904..7482
                     /locus_tag="ACG8H7_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFP8984331.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7577..7714
                     /locus_tag="ACG8H7_002261"
     CDS             7577..7714
                     /locus_tag="ACG8H7_002261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984332.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2346 a         1401 c         1556 g         2544 t
ORIGIN      
        1 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcttattttg attagaactt
       61 gcgaaaataa accggttaaa gtgtgtctag cgcaagagat ttgctataat agacgataag
      121 ggtaaaatgt aacgagaagg ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg
      181 tgatctgcca taccatgtat tagcaaaaga aaaagttgat tttttatcaa tgaatctcca
      241 attgaatgat caagagttag tagatgacct cggaaagaca ttcaattatg accgctacat
      301 gaatcagctg aaaaatggtg cgatgcctac gacatcacaa atcaatgttg gccgatattt
      361 agaatttttc cgttcttatg cggaaaaaca gattcctgtt ttatatgtgg ctttttccag
      421 tggattaagc ggctcttaca gtagtgcgct tcaagcggtt gaattgctga aggaagaata
      481 tgaagaccct gagatctatg tttttgatac aaaagctgca agtattgggg aagggctttt
      541 agtattggaa gctgtaagat tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg
      601 gctggaaagt aattatttga aagtccattc atgggtaaca gtagacgatc tgaaatatct
      661 agaacgaggc ggtaggattt ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa
      721 gcctattatt acggtggata aagaaggaag gctgacgaat gttgacaaag ttcgaggaag
      781 aaacaagtcg atccagacaa tcgtcaatga gacggtaaaa acaatcgttg caccacttga
      841 tcaaatcgtt tatgtggctt acgctgggga tctagagagc gcggagaagg caaaacggct
      901 tttggaagag cagataaaga tgaaagatgt gaagctttat cccttaggac caacgatcgc
      961 cagtcacaca ggctatggct gtatcgcgat tttttcgatg ggaatcagtc gataaaagca
     1021 gtaactcatt ctgtaaaaaa gcccagaaat tgattgtaac aaaaacagcg actcttaaaa
     1081 gatagatgag catcctatct cttagaggtc gctgtttttt tattttttaa gctaaatagg
     1141 ccattatgcc cataactcgt tcaattgttt ttgaatatct gcattttcca aaaattcatc
     1201 atatgtcgta tcagctcgat cgatcacacc tttatccgaa acggcaatga tgcggtttgc
     1261 caatgtttgg ataaattgat ggtcatgtga cgcaaataag attgagccag taaatgccat
     1321 caagccgtca ttcaatgctg tgattgattc taagtctaaa tggttcgttg gatcatctaa
     1381 tacaagtacg ttagctttcg aaagcatcat ttttgaaagc atgcaacgga ctttctctcc
     1441 accagataaa acattcactg gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa
     1501 gaagcttctt aagaaggtgt tatcatcttc ttctttactt gcatattgac gcaaccagtc
     1561 taaaatcgtt agatcagtat cgaattcttt cgttgtatcc tttggtaaat aagaattact
     1621 tgttgtcaca ccccaacgta ctgttccagt atctggcgta atttcaccca tgataacttt
     1681 gaatagcgtt gttgttgtta tatcattttc tgcgacgaaa gcgaccttgt cttctttgct
     1741 caaattaaaa gatatatcat ctaaaatttt ctttccatca atcgttacac ttacattttc
     1801 tacctgcaat aagtcgtttc caatctcacg ttcaggtttg aatccaacga atggataacg
     1861 tctagaagaa ggttggatat cgtctaaagt aattttttca agcatttttt tacgtgaagt
     1921 tgcttgtttt gattttgatg cattcgcact aaagcgggca ataaattctt ggagttcttt
     1981 gatttgttct tcttttttgg catttgcgtt tgcttgaagt ttagccgcta gttgactaga
     2041 ttccaaccaa aaatcataat ttcctacata aagtttgatt ttaccaaaat ctagatccgc
     2101 catatgtgta catactttgt tcaaaaagtg acggtcatgg gatacaacga tcacagtatt
     2161 ttcaaaattg atcaaaaatt cttccagcca attgatcgat tggatatcca gaccgtttgt
     2221 tggttcgtct agcaaaagaa catcaggttt accaaatagt gtttgtgcta ataacacttt
     2281 taccttttgt ccaccagtta attcactcat ttgctgatgg tgcaattctt cagggatgtt
     2341 cagaccttgc agtaaaacag ctgcttctgg ttcagcttcc catccattaa gctcagcaaa
     2401 ttctccttct aattcagctg ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta
     2461 aatcgcatct ttttctttca gcacttcata cagacgcttg tgtcccatga tgactgtttc
     2521 aagtacagta tagtcttcat agtcgtaatg gttctgtttc aatacggcca tccgttcatc
     2581 tggtcccata gtaacggaac ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa
     2641 cgttgattta cctgcaccat ttgcaccaat caagccatag cagtttccgg gggtaaattt
     2701 aatattcaca tcatcaaata gtttgcggtc tgaaaagtgc aaactcacat catttacagt
     2761 aatcaatttt tttcctcact tttatcatta ttagttttga caagtcttat agaattatat
     2821 aggggaaagg taaaagattc aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa
     2881 tgaaaaaaat aaaaataaaa gcggagcgaa aatagctact ataggattta ttagtttgca
     2941 acagcataat ttttgacgtt atttataaga gggagtacta taaaactgta aaagaaatga
     3001 aagaaggatt gaaaatggat gaaaaaagaa ccatcgactg gggctcattg attttgggaa
     3061 ttttatttgt acttgtctca ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa
     3121 ttgtagtagt ctttgcgatt tttgccttta ttaaagggat atttgaactg tttgtacgga
     3181 acagattaaa agagctaaca ggatataaag gaaagatgcc cttaataatc gggatcatcg
     3241 atattctagt tggcgtcttt ttcttattca atattggtgc aggagtcgca gcattgcctt
     3301 ttgtttttgc agtatggttt attgctgatt cagttttagc gttatttact gctgacttgg
     3361 caagaggagt aagtgaagga tattattggt ttacgatcat cgtcaatatt ttaggaattt
     3421 tattaggaat tttcttattg tttaatccaa tttcttctgc gttgacatta agctttttag
     3481 ttggattcta ttttatgtta tttggtatca cacatatcgt ctatgcattt agataagtaa
     3541 aatcaaacaa ataaaaaagc cggcttccga ttacagtttt actgttgtaa ttggaagccg
     3601 gcttttttat catggacaaa tatgcacgag gtgattttta ggtgtcgaga caaatttatg
     3661 caaaaatgtg agaagctata tacacaaatt ttatcttaat actcgaagca tagcgcagtt
     3721 gtcacaacct cttttcatat aattcattca atactggcaa atcatggact tcatattttt
     3781 ctttgaatgc ttggatttct tcttcagaat attttcttgg atctaaatgc agatcttcta
     3841 ctggcaatgg gcgcttgtta ttgatcttca catcgattac tacaggacga tctgattttg
     3901 cagctgcatc aaaggcaaat cgaagttgcg agtattctgt gactgtaaag ctatttgccc
     3961 cgagcgcttc tccaacttta ccgaaatctg catcttctaa aaatacacca aatttctttt
     4021 gttccgtatc ttcttgttct gcttcgatga aaccaaaaga atcattcgaa aagacaacat
     4081 taataattgg aagctgatat ttgacttgtg taatgatatc ttgcatgacc atagcaaacc
     4141 cgccatcacc acttaaggta aatacttgtt tttctgggta gcttagttgt gcggcaattc
     4201 ctcctggcac accattaccc atagttgcga accatccaga tgtcgtatgt ttttgtttac
     4261 cgttcatatt caataaacga atagaatgga tagtcgtgtt accgacatct gtgacgaaaa
     4321 tcgcatcgtc ttcagcaata cgattgattt ctttatagac agcctctgga cgaatcggtt
     4381 cttcctctcg atcttcaaaa gatttcaacc aatttaccca tttttcttta ttttcttgat
     4441 ttgctaggta ccagctatca gcagggcgag catcccctaa ctcgacaagt ctttgcaaag
     4501 ctgtgtttgc atcaccgagg atcgacaaag atgtttcatg gcgtcgtcca aattttgacg
     4561 catcgatatc gatttggata aattcagcag ttggattaaa aaatgcacgt ccaaatggga
     4621 agtcgctacc gacaaataag atcagatcgg cttctgccaa tgcttcattt ccgggtttag
     4681 tagcgacacg tccagcaaaa ccaagaaagt tttcataatc atcaggaata attccttttg
     4741 ctagaacgga agcgacaaca ggaactgaaa aatgttctga gaattctttg atttgcggaa
     4801 atccgtttcg cgttccttga ccaacataaa gaactggttt ttttgctttt tcaagataag
     4861 gcaaagcagc tagcaaatct ttttcttctg gtaatatcac acttgttttg tgagtgtgag
     4921 cggtggagaa aggttgttcg tcaatttctt caaaaccaag atcaacaggg atagtcacga
     4981 tagccacgcc tttatgctca taggcggctt taatggcttc atctactaca tgcggaagac
     5041 tttgtggtgt catgactgta cgattgtata cgctaacatc tgcgaaaata ggattttcat
     5101 ttagttcctg gaaagagtta tagttcatgg atgtagaagc aacttgtcct aagaacgcta
     5161 ctagaggcac atggtccatc tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag
     5221 gtcctgcaga gccgaagaca gcgccgactt tccccgttaa ttttgcatct gcagcggctg
     5281 cgagtgcgcc gacttcttca tgacgaactt gaatgtagtt gatttcggtt ttttcttgat
     5341 atagcgcatc cataattgaa ttgaaagatc ctccgggaat gccgtaaata tggtcgatac
     5401 cccagctttc aagaactttt accattgcga ttcctgcatt gatttttgac atggtgtttc
     5461 actcctttag caatgatgat acataaattt tttgtatcta gagtatagaa ctttttatcg
     5521 aaaatcccaa ctaattcgtc ttttcacaaa aatgtagtca tgtttttggg aatatgctag
     5581 agtagtacat agatagattc aagagagggg aatggcatgg ttgaatggct attgagactt
     5641 gaaccttggc agcaagcact tgctgggaca gcttttactt attttatgac agctcttggt
     5701 gccgggttgg tttttttctt taaagaaatc aaaaaagaag tgctaaactt gatgttagga
     5761 tttgcttctg gtgtcatgat tgctgcaagt ttttggtctt tgttagatcc ggctattaca
     5821 aaagcagaag aaaatggaga tattgcttgg ctagttgtaa gtattgggtt tggtttaggc
     5881 ggtgtgtttt tatatatggc agataaaaca ctgccccata tgcattttgg accacaacat
     5941 gaagcagaag gattgcctac ccatttaaaa cggacgatct tgcttgtttt ttctattaca
     6001 ttgcataaca ttcctgaagg tttagccgtg ggggtagcat tcggtgcagc agcaactgcg
     6061 gataatccga cagccgctat attagccgct gtttcggtgg cgctaggtat tggtatccaa
     6121 aacttcccag aaggagcagc tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa
     6181 aaggcctttg tttacggaca agcttctgga atcgttgaac caattgctgg agtgattgga
     6241 gcgctactag taacgaaagt ggagctgctg cttccttatg cacttgcttt tgccgcagga
     6301 gcaatgattt atgtcgtggt ggaagaattg atccctgaag cacagcagac attgtccagc
     6361 aaacgtcatt ttgcagtttt tggcgtcatg agtggattta tcattatgat gattttggat
     6421 gtggcattag gataaataaa aaaaatatat atgatatatc aaatagggcc gcgaacttat
     6481 atcgcgaccc tattttttta gtaagttata tcataatgaa gtgaatggta tcatacatta
     6541 ataatagttt tttctatgtg tatttccttt gatccagcga acaagccaga tgataagtgc
     6601 gattgggatt agaactttga ccgcaaacca taaaagcccg cttaggacac tgacaaaaag
     6661 actgatgacg aagctagctg caattagcac taaaagaacc atgaagatat ttaagaacat
     6721 atttttttga cgcatacaaa accctcctct tgttcattct ttactataga aaaagtataa
     6781 cagggattgt ttaaaaacac ctccaactga agactgattt ttacttcatc tacttaaatg
     6841 atcaaacaat tgctttttct ctggtttatc atctaaaata gagcggaaaa ggaggagaat
     6901 atcatgccta aaaatcgtct tgaagcattt acagatgctg taatcgcgat tatcatgact
     6961 attttagttt tagaacttca tgaacctaac ggagacacgt gggaggcgct cgctgcatta
     7021 ggttaccgat tttttatcta tattgtcagt tttatcattt tagcgattta ttggaacaat
     7081 catcatcatt tatttcaagt tgtacataag ataaatggac gggtcttatg ggcgaacagt
     7141 ttctttgttt ttgcgctttc gctttttccg tttgcaactt catggatcag tgagtacatc
     7201 aatagcctgg ctccagaaat gacttatggt tttgttacct tgaccgctaa tattgcgtat
     7261 tatatcttgg caaaagaact tgtcaaagca aataaaggcc acgagaaagc attgaaggcg
     7321 atgttgaata attacaagaa atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc
     7381 ttagggtatt tagtagcgcc agtagccgtg ctgatcgcaa acatcgtgat tctagctgct
     7441 tggatcatcc caagtcgtac catcgaatca caatatcggt aaaatgatcc gtgtttaaaa
     7501 tttaagagaa aaatatagaa aaaataaata aacgatcaaa tatccgagtg atactagtat
     7561 tcaaagaggg atgcagatgg atcaaaaaag aattcatgta ggactgatta ctagttttgt
     7621 cacgatgctt atttatttgg atcaaggtca tttgacttgg acagtagttg gtgtcgtagg
     7681 atttttgaca ttattcacag caataaaaaa ataaacatct ttatgtaatc tgatgttcta
     7741 tctatttaag aaaagcatgt ttacagcagt atgattctgt tgtaaacgtg tttttatttg
     7801 tggaaaattt aatttttaga aaagtgtatc gaaaaataga tagttag
//
LOCUS       DAZKAS010000125         7835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000125 DAZKAS010000000
VERSION     DAZKAS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.127"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACG8H7_002262"
     CDS             complement(21..995)
                     /locus_tag="ACG8H7_002262"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP8984333.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACG8H7_002263"
     CDS             complement(1075..1950)
                     /locus_tag="ACG8H7_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFP8984334.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACG8H7_002264"
     CDS             2080..3987
                     /locus_tag="ACG8H7_002264"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFP8984335.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACG8H7_002265"
     CDS             complement(4528..7806)
                     /locus_tag="ACG8H7_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFP8984336.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2208 a         1658 c         1460 g         2509 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaag
//
LOCUS       DAZKAS010000126         7787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000126 DAZKAS010000000
VERSION     DAZKAS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.128"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACG8H7_002266"
     CDS             <1..342
                     /locus_tag="ACG8H7_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984337.1"
                     /translation="IFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPP
                     KSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNK
                     NNNFYFYRNKL"
     gene            complement(468..1145)
                     /gene="nth"
                     /locus_tag="ACG8H7_002267"
     CDS             complement(468..1145)
                     /gene="nth"
                     /locus_tag="ACG8H7_002267"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP8984338.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1152..1850)
                     /locus_tag="ACG8H7_002268"
     CDS             complement(1152..1850)
                     /locus_tag="ACG8H7_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFP8984339.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2118..3302
                     /locus_tag="ACG8H7_002269"
     CDS             2118..3302
                     /locus_tag="ACG8H7_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFP8984340.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3308..3751)
                     /locus_tag="ACG8H7_002270"
     CDS             complement(3308..3751)
                     /locus_tag="ACG8H7_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP8984341.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3977..4264)
                     /locus_tag="ACG8H7_002271"
     CDS             complement(3977..4264)
                     /locus_tag="ACG8H7_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFP8984342.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4435..5787)
                     /locus_tag="ACG8H7_002272"
     CDS             complement(4435..5787)
                     /locus_tag="ACG8H7_002272"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFP8984343.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6060..6506)
                     /locus_tag="ACG8H7_002273"
     CDS             complement(6060..6506)
                     /locus_tag="ACG8H7_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP8984344.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6742..7668)
                     /gene="cysK"
                     /locus_tag="ACG8H7_002274"
     CDS             complement(6742..7668)
                     /gene="cysK"
                     /locus_tag="ACG8H7_002274"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP8984345.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2106 a         1583 c         1270 g         2828 t
ORIGIN      
        1 attttttctg tacttttaag cactactctt aaaggaaaat ttatggaaag aagctctcat
       61 gcaatgattc tcactttttt atggttgttg ttcaccagaa tctatatttt tctgatgagc
      121 ctgaaaccgc ccaaatccat tagtttatgt ttagttataa atggtatttt tttattatca
      181 cttatcattt ttattacacc tttagataga tacgtcactt atttatataa tcctttggaa
      241 tactggactt attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc
      301 aataaaaata acaactttta tttttatcgc aataaattgt aataagttac catcctagct
      361 actgagtatg tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat
      421 aataacaaga taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt
      481 ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat
      541 agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa
      601 atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct
      661 tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc
      721 acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct
      781 gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca
      841 tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac
      901 aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg
      961 ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc
     1021 tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca
     1081 tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt
     1141 aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt
     1201 tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct
     1261 tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg
     1321 taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc
     1381 ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc
     1441 gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt
     1501 ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg
     1561 taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat
     1621 ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc
     1681 atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt
     1741 agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga
     1801 ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct
     1861 ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat
     1921 ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt
     1981 tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag
     2041 aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt aacattattg
     2101 cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg
     2161 tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa
     2221 cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg
     2281 tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg
     2341 ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg
     2401 gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag
     2461 gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca
     2521 aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag
     2581 ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt
     2641 tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt
     2701 cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga
     2761 tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc
     2821 ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg
     2881 gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc
     2941 gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc
     3001 tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg
     3061 ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat
     3121 ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat
     3181 ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc
     3241 ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat
     3301 agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg
     3361 tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt
     3421 gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga
     3481 tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt
     3541 ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt
     3601 taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg
     3661 caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat
     3721 attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat
     3781 tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa
     3841 accaagaaaa aaaggggttg acaaagcttg tcataacccc tttttctgat taaaagatag
     3901 taaagagaca gtcatcggta attgtgatga ttttttgtca tcacttaagg atgagcggcc
     3961 ttttcaaaaa ctgtctttaa agatcatcat cagtttctag cacatatggc tgaccttcat
     4021 atgtgtactc tgtattcaat gtgccgtcat tttcatctga gtagatgcgt ctactgaaag
     4081 gaaaacgatg agaaacatta tctttggcgt catttgctgg catcgtgtaa tattcctctt
     4141 gcgtttcatt caactgagag agtacttgat ctgccaactc atcaatattg atcgttaatt
     4201 tcttcgaagc acttttataa tcttctaaaa atgcatcttg ttggacaaaa tattctttat
     4261 tcatcttctt tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc ttttatgtaa
     4321 cagctttatc tttgctgctt attgtgacaa aaaattcgaa gaaagacaac taattgtact
     4381 tttcacaaaa aaagacagag ggtcgtgaca aaattgtgtc acaacccttt ttctttattg
     4441 tgcagtttcg atttcgccta gcgctgcttg tgcaagtagg ttcaagtaat tccatgggcg
     4501 gtcaaaatgt ggttggaaga agaaatctga aagtgcgaga tcttctacag tcattttatt
     4561 ttgaacagct aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg acatgaactg
     4621 agcacctacg atacgattcg ttcctttttc atagactagc tccatcatta ctttttcagt
     4681 agttggcatg aactctggac gataattatc ttcaaataca gtcgcttgga cttctagatc
     4741 gttcaaagtt gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc caaataggta
     4801 aagtcctgaa gttccttgag taccacgata cgccatttta tgatctgtca agttatttcc
     4861 cacaagcaaa ccttgacgaa cggcatttgt tgccaaagga atatagtttt gcgtgccact
     4921 tggattgtag ttgactactg cagagtctcc agctgcaaaa atgtctggat tacttgtctg
     4981 catgtattca ttcactttga ttgccccgtt tggcagtgta tctacttttc cttcgatcag
     5041 ttttgtattt ggacggaagc ccacacataa aatgaccata tctgtatcaa atgtatctgt
     5101 tggtgtagct acttgtttca cttttccttc ttcatctgtg ataaattcag taacattttc
     5161 gcctagcgca agagtaacac cgcggtctgt caattctttt tctaagatat ctgtgaatgg
     5221 tttatctaga tatttattca agatgcgatc taatccatca atcaatgtca cttgttttcc
     5281 agattcgaca aaagcttcta ctaattcaat accgatatat ccaccaccaa caattactac
     5341 acgttttgct tctttcgctt gtgcaataat ttcatttgct tgattgtagt ttttgcatag
     5401 gagtacattt ttcgcatcga ttccactaat cggtgggata ataggccatg aaccagttgt
     5461 catcactaat ttatcataag aaaccgtttc tgtttctcct gttttcagat cttttgccgt
     5521 tactgtttta ttttctgtat cgacgtctgt tacatcgtgt tccattttaa ctgttgctcc
     5581 taatgaagat aattcttcag gatttgagta gaacaagcct gctgggtcct ttactactcc
     5641 gccgacataa agtgcgatac cgcaagataa gaaagaaata ttgtcatttc tttcatatac
     5701 tgtcacttct gcttctggat gattttttaa aatacttttt acagctgcag tacctgcatg
     5761 agtacagcca attactacta ctttcattta tggtccctcc gattaacatt ttgtccttcg
     5821 acaaaactac tataacagaa ataaaacgga tacaacagtt taatgcttgt gaattcacaa
     5881 aggtttgttt gtagtttcat tccttttcaa gcataagaac ccaatatcta caagattctt
     5941 attttaataa tattcacttt tatatctaag attgtgaata aaagacaaaa aaataaaaaa
     6001 gctagcgttt tttttacaca gctagctttt tttaggtata acacttgtag aaactgtttt
     6061 tacatttgcg ccttttttgc tgactcttta caatccggac agagtccata caattccaac
     6121 cgatgttcat taatctcaaa atcagtcagt ctgcttgcag ccatttctat atcttcaagc
     6181 cctgggtaat gaaaatcaac aatcttaccg cagttttcac aaattacatg ataatgacgc
     6241 tttgaactga aatcaaatcg gctagaagca tcgccgtact tcatttcttg aacaaatcca
     6301 atcgaagtaa ataagcgaag gttattgtaa acagtcgcaa cactcatatt tggaaaccga
     6361 tgttcaagtg ctttataaat ctcatcagcc gttggatggg aatgatgttc aatcaaatat
     6421 tctaaaaccg cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa cttttcgata
     6481 acctcttcga ctaacttatt ttccatgtaa acaccccacg ttgttattct cgtcttatca
     6541 tactattttt acattttaaa atccacttaa catgaaaaga aaattcaatt ttattgtatt
     6601 ttaatgaaaa aagacgtaaa acagtcgcgt tcactattct acgtctctat tcaaataatt
     6661 tttctcatca taaatttttg tgtgttcttt tcatctttaa aagtttttta cataaaccta
     6721 tctggagaaa aaccaagtgc cttatatctc ttggtaaagg gctgtagata aataacgttc
     6781 accattgtcg ggaaccacag ctaatacctt cttgcctttt cctaattctt ttgcgacttt
     6841 tagcgcggca gcaattgcag cgccagatga gatgcctact aaaatccctt cttttcgccc
     6901 aacttctcta gctgtttcca tcgcttcgtc tccactaata gaaaggactt tatcatacac
     6961 agaagtatcc agtgttttag gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc
     7021 cgcttctttc ccttctaaaa tagcagattc tgctggttct accccgatta attcgatttt
     7081 cggatacaca cgttttaatt ctcggccagc gccagtgatc gttcctcctg taccgatacc
     7141 tgcaacaaac gcgtccaagc catttactcc aaatgcttga tgtatctcag gaccagtcgt
     7201 cttctcatgc acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt
     7261 ttcttttgcc aaacgttctg cttctctgat tgatccagaa ataccatccg caccaggagt
     7321 caaaatcaac tctgcgccgt accctttcat caataaacgt ctttcgatac tcatcgtctc
     7381 aggcatcacg atgattactt tatatccttt tgcaactcct accatcgaca atccgattcc
     7441 tgtatttcca gaagtaggtt caatgatagt atcacctggt ttcagtagac catcgtgttc
     7501 tgctttttct atcatactta aagcaatacg atcttttaca cttcctcctg gattaaaaaa
     7561 ctcaagcttt acaaaaacat ctgctgaatc ttctggtacg attttattta atttgacgat
     7621 tggcgttttg ccaatcaatt cggtgactga gttatagata gctgtcatct tcaattcctc
     7681 cttgtctttg ctcttcttac ttatcataat acaatctgtt catttatggc gagtgattcg
     7741 tctttgacta gcaaaaaaaa gacaaagata taccatatat ggtaatt
//
LOCUS       DAZKAS010000127         7688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000127 DAZKAS010000000
VERSION     DAZKAS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.129"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(115..639)
                     /locus_tag="ACG8H7_002275"
     CDS             complement(115..639)
                     /locus_tag="ACG8H7_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP8984346.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(632..1105)
                     /gene="tsaE"
                     /locus_tag="ACG8H7_002276"
     CDS             complement(632..1105)
                     /gene="tsaE"
                     /locus_tag="ACG8H7_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP8984347.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1228..2208)
                     /gene="pta"
                     /locus_tag="ACG8H7_002277"
     CDS             complement(1228..2208)
                     /gene="pta"
                     /locus_tag="ACG8H7_002277"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP8984348.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2361..3041)
                     /locus_tag="ACG8H7_002278"
     CDS             complement(2361..3041)
                     /locus_tag="ACG8H7_002278"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP8984349.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3167..4036
                     /locus_tag="ACG8H7_002279"
     CDS             3167..4036
                     /locus_tag="ACG8H7_002279"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8984350.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4062..4577
                     /locus_tag="ACG8H7_002280"
     CDS             4062..4577
                     /locus_tag="ACG8H7_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFP8984351.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4624..5187)
                     /locus_tag="ACG8H7_002281"
     CDS             complement(4624..5187)
                     /locus_tag="ACG8H7_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8984352.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5242..5421)
                     /locus_tag="ACG8H7_002282"
     CDS             complement(5242..5421)
                     /locus_tag="ACG8H7_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984353.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5498..6007)
                     /locus_tag="ACG8H7_002283"
     CDS             complement(5498..6007)
                     /locus_tag="ACG8H7_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984354.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6103..6483)
                     /locus_tag="ACG8H7_002284"
     CDS             complement(6103..6483)
                     /locus_tag="ACG8H7_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP8984355.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6813..7562
                     /gene="pnuC"
                     /locus_tag="ACG8H7_002285"
     CDS             6813..7562
                     /gene="pnuC"
                     /locus_tag="ACG8H7_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP8984356.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2196 a         1593 c         1364 g         2535 t
ORIGIN      
        1 gaacaccgtt tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg
       61 tgtttttctg ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc
      121 aatcaaataa ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct
      181 aggcatgacc gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt
      241 acgaacttga acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat
      301 gatttcttct agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat
      361 accgacttct cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc
      421 aataatcgtc ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata
      481 atccaactct tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa
      541 aaattctgtc tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg
      601 cactgcttcc cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta
      661 gaattcgctg tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct
      721 tttctaaatc tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt
      781 cttctaatag atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta
      841 accctaaatc tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc
      901 gaccttgact atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc
      961 ctaatccttg ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca
     1021 atatcagatt gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca
     1081 ttgttaatcc tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca
     1141 gtatatcgcg taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc
     1201 tgtttttggc tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat
     1261 cgataattta tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt
     1321 caagccttgt agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata
     1381 gccgatattt cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc
     1441 tgaatctggc gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc
     1501 gccgtcgatc atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt
     1561 gtctacttct ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg
     1621 ttcgatatcg aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg
     1681 cgcagttgga ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc
     1741 acgtaccatg atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg
     1801 aagtgctggg cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc
     1861 aatccccata tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc
     1921 ttgttctttg gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca
     1981 cttatcgtaa ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg
     2041 cgctgcattt cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc
     2101 tttcaaccgg gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt
     2161 gatcccacgg cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc
     2221 agacgttttt atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc
     2281 tgtatcagtc aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat
     2341 aacaggaaag cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct
     2401 cctaacgcaa ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga
     2461 cttgcagata acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc
     2521 attttgattt tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt
     2581 tcatttaatt tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa
     2641 tacgcttgcc cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc
     2701 catgacacta gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta
     2761 tagatatttc ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca
     2821 tgagcttgat tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca
     2881 taaggagtca attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac
     2941 gcatattctt tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag
     3001 gacaaaactt cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa
     3061 aaagaataac tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt
     3121 aatatggtaa actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat
     3181 tgcatcagat atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc
     3241 cgaggcgata cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa
     3301 ctatcaagaa gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt
     3361 gaacggggca caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa
     3421 taatcaagca atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc
     3481 aaccaacgat ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat
     3541 ggtggctacc catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa
     3601 gctggagtta ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa
     3661 tctggaagtt ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt
     3721 tggaaagcag atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat
     3781 cgaagtgaac cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg
     3841 tgggatttct ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct
     3901 gcaagtagca ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc
     3961 caaatataca acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat
     4021 tgcagaagac ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc
     4081 aggaaaagca gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc
     4141 tgtttttgat ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga
     4201 atggcgatct cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc
     4261 tgtataaaga gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact
     4321 tttattatat tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc
     4381 aaatccatca tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag
     4441 attactatgt attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg
     4501 cggttttaga ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc
     4561 gacttaactt aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc
     4621 tttttagata ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac
     4681 aactttgaag tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact
     4741 tagaccgatg acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg
     4801 cgaaccgatg ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc
     4861 gttgggaaac gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc
     4921 taagataggt accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc
     4981 aactcctgca actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata
     5041 atttggatca gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt
     5101 caaaaatggc agttccatat cttttagaat cactaagatc aatggggcga tttctggagc
     5161 atcttctttt ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca
     5221 gaaaaagaaa tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata
     5281 accatgagta acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc
     5341 tttatcatta tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt
     5401 agggcatttc attttttcca tgttatctca acttcctttc atctttactc tcttagctta
     5461 ctcttatatt gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac
     5521 ggtcctgttc tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa
     5581 gtgtccactc tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg
     5641 cttgcggttg tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt
     5701 ccgtgtcatt tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt
     5761 cacaaaaaaa ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg
     5821 caaaatgaat agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt
     5881 ttttcaatga gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga
     5941 gcagtcccgt cataccggta atccctatta cgatgagggc agcaacacca aacatcattt
     6001 ttttcattct tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga
     6061 aaaatcgaat gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa
     6121 tggcaatccg tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct
     6181 aatcggttct gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc
     6241 tactttgaaa gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc
     6301 attagcaatt ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc
     6361 atctcgttct ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa
     6421 atcacgtgat ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt
     6481 catatgagca ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg
     6541 taaatgtgtc agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa
     6601 aaacagaaag taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa
     6661 gaaattatat tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg
     6721 aaagactagt tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac
     6781 actacattta gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc
     6841 atcagctaaa aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc
     6901 aggtcatgac tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta
     6961 ccacgcttgg tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt
     7021 taggtatttt gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa
     7081 gtatcggtga acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag
     7141 aatggaatca taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg
     7201 caatcatcag tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag
     7261 atgatcctcg gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca
     7321 tcagtttctt gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa
     7381 tggtcctttg gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca
     7441 gctctgttta cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa
     7501 aagaacgtga acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat
     7561 aaaaaaactg agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
     7621 taaacggcgg aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
     7681 aagcaatt
//
LOCUS       DAZKAS010000128         7601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000128 DAZKAS010000000
VERSION     DAZKAS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.130"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG8H7_002286"
     CDS             complement(138..428)
                     /locus_tag="ACG8H7_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984357.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG8H7_002287"
     CDS             complement(546..806)
                     /locus_tag="ACG8H7_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984358.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG8H7_002288"
     CDS             complement(943..1356)
                     /locus_tag="ACG8H7_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFP8984359.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACG8H7_002289"
     CDS             complement(1812..2297)
                     /locus_tag="ACG8H7_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984360.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACG8H7_002290"
     CDS             complement(2436..2651)
                     /locus_tag="ACG8H7_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFP8984361.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACG8H7_002291"
     CDS             2875..3675
                     /locus_tag="ACG8H7_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP8984362.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACG8H7_002292"
     CDS             3694..5562
                     /locus_tag="ACG8H7_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP8984363.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACG8H7_002293"
     CDS             5555..6046
                     /locus_tag="ACG8H7_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP8984364.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACG8H7_002294"
     CDS             6034..6588
                     /locus_tag="ACG8H7_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP8984365.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="ACG8H7_002295"
     CDS             6606..7601
                     /locus_tag="ACG8H7_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP8984366.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAZKAS010000129         7406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000129 DAZKAS010000000
VERSION     DAZKAS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.131"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..739
                     /locus_tag="ACG8H7_002296"
     CDS             <1..739
                     /locus_tag="ACG8H7_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8984367.1"
                     /translation="FKFLFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRN
                     KRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYW
                     PFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLM
                     TILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGL
                     YQSVVGFVLLMITNTIARRFDSDSALF"
     gene            751..1671
                     /locus_tag="ACG8H7_002297"
     CDS             751..1671
                     /locus_tag="ACG8H7_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP8984368.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1712..3169
                     /locus_tag="ACG8H7_002298"
     CDS             1712..3169
                     /locus_tag="ACG8H7_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP8984369.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3514..4128
                     /locus_tag="ACG8H7_002299"
     CDS             3514..4128
                     /locus_tag="ACG8H7_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFP8984370.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4121..5845
                     /locus_tag="ACG8H7_002300"
     CDS             4121..5845
                     /locus_tag="ACG8H7_002300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8984371.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5842..7293
                     /locus_tag="ACG8H7_002301"
     CDS             5842..7293
                     /locus_tag="ACG8H7_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8984372.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2526 a         1232 c         1472 g         2176 t
ORIGIN      
        1 tttcaaattt ttattttctt cggatcaagc atttctcatc actaaaaata caattttata
       61 taatgtagca tttatccttt tgaatctgct gatctcagtc gtatttgcaa tcattatgag
      121 cgagttgaga aataaacgac tcgttaaagt ttatcaaacg atgtccttac taccttattt
      181 tctatcttgg gtcattatca gttactttgt ttacgctttt ttaagtcctg acaaaggaat
      241 attcaatcag tggatcactg gacatggagg agaagctgtc aattggtata atgaaccgaa
      301 atattggccg tttatcttgg tgttcattgg tacatggaaa gggattggtt ataacagcat
      361 tatttacttt gcatctgtta tgggaatcga tccgacttat tatgaagctg ctatggttga
      421 tggagctagc aaatggcagc agatcaaaca tgtgacgata ccacaactta ttccactaat
      481 gacgatttta acgattttag ccgtaggaaa cattttccga gctgattttg gcctgtttta
      541 taacattcct aggaattccg gtgcattata tgatgtgact caagttttgg atacgtatat
      601 ctacaatggc ctgacatcca caggagattt tggtatgacg gctgctgcag gactttacca
      661 atcagttgtc ggctttgtct tgttgatgat cacaaatacg atcgcccgac gttttgatag
      721 cgattctgca ttattctaag gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa
      781 gtccgctctt tcaataagac ggctaatttt ttctctaata taatgattgc actctttgct
      841 atttcctgta ttttgccatt tatatttgtt atcgccatct cttttaccga tgagtccata
      901 ttgtcgactc acgggtacag tttttggcca gaaaagttca gtatatttgg ttatacgtac
      961 ttgttcgacc agatgcagga taaaattttc caagcgcttt tcgtcactgt acttgttact
     1021 gttttaggta cactgatcaa tagcacggct acttctttat atgcttatgc gatttcacgg
     1081 tcaaactttc cattccgtcg tttttttacc gttttttgtt tgattacgat gttgttctca
     1141 ccagggatgg tggccaatta tcttgttatg acaaatcttc ttcagttaaa agatacgatt
     1201 tgggcattga tcttgccaat ggctgtcagt cccttcaata ttattgtgat gcgcacattc
     1261 tttaaacgtt cggtctctga ttcgattatt gaatcggcac ggatcgatgg agctagcgaa
     1321 ctgcgaatat ttgttcagat cgttttaccg ttagctgttc caggtattgc gacaatcagt
     1381 ttatttgccg cattgggtta ctggaatgac tggttcaatg ctttactata catccaagat
     1441 gataagctag ttccactaca gtatcttttg atgaaaatcc aaagtaatat tcaatatctg
     1501 acacaaaatt caggagcagg aagccaattg acaggcggat tagcctcagt tccaggagaa
     1561 tctgccagaa tggcgatcgt agtcatctcg acgttgccta ttgcgatcag ctatccattt
     1621 ttccagaagc attttgtcaa aggtctaaca atcggcggag taaaagaata aagaaacaaa
     1681 aatataaatc ataaaattag gaggaagaaa aatgaagtta tggaagaaac tagcctttac
     1741 aggtgtttca gcgttgttgt tgggaacatt agcagcatgc ggaggttctg gttctaaaga
     1801 ccaggcagaa gctagcaatt cggacacttt gcagatgtat caaatcggag ataaaccaga
     1861 taatttcgat caattgatgg aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa
     1921 agtcaatatc aattacattg gctggggcga ttacgaaaag aaaatgaacg tcatcatttc
     1981 ttccggggaa aattatgata tcgcctttgc gaacaactat gtttccaatg cccaaaaagg
     2041 agcctttgct gatttgactg aattagcgcc taagtacgct gaaaaagctt ataatgattt
     2101 agatgaagca tatattaaag gaaatctggt caatggaaaa ttatatgctt tcccagtcaa
     2161 tggaaatgtc tttgctcaac aagtacttac ttttaacaaa gctttactag ataaatacga
     2221 tctttcaatt gatggaatcc aatcttacgc tgatgcagaa aaagtcttga aggaattcca
     2281 tgaaaaagaa ccaaacacgg cagcttttgc cattggacaa ggcttcaaag tccaagggaa
     2341 ctttgattat cctttaggga atacgttacc atttgctatt gatctggatg gagatgattc
     2401 gaaaatcatc aaccaatacg ataatgaaac gtatatcgga ctgttacgta caatgcatag
     2461 ctggtacaaa caaggactca ttccaagtga cgctgcaacg agcaataagg attatccact
     2521 agaaggaaat acttggctaa tgcgtggaga aacacaagga ccgtatgatt atggcgatac
     2581 aattttgaca aatgcagcaa aacaagaact tgtctcaaaa gcaattactg ttccactaaa
     2641 atctacagct caagcacaaa tggcaaattt tgtcgtatca aatacttcaa aaaataaaga
     2701 aaaatcagta gaacttctag ggctgcttaa cagtgatcca gaattattga atgggcttgt
     2761 ttgggggatc gagggagaag catgggaaaa agtcgatggt tcagatgata aaatcaaatt
     2821 gctagatggt tatcagccaa atacacatat gtctgcttgg aatacaggaa acaacaagat
     2881 tctatataca caagagtcta ttacggatga catgattgcc aaacgtgatc aatcgattgc
     2941 tgatgcagaa acttcaccga ttttaggatt cagcttcaac actgacagtg tcaaaacaga
     3001 attaagtaat atttctaatg tcatgaatca atatttagat ggattgaata cagggactgt
     3061 tgatccagat gaaacattac cgaaattgaa agacgctttg gacaaggctg gctatgataa
     3121 ggtattgaaa gaaatgcaaa aacaatatga tgagttccga caagaataat aatgaataaa
     3181 gacaaagggg ttgtgacaaa agacgtgtca caacctcttg ttccgaataa acggtgttca
     3241 aaagccagtt tctcggtatt aagctaaata tatgtaaaaa tgtggaaagc catattcaca
     3301 aaatttttct tactactcga aactaagcgg ctatttcaca aactcttggt aaacggtgtt
     3361 caatcagcta accctcggta ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc
     3421 gtttgtttct ctaataatca ggtcaaggtg ctgattgccc aacctttttt tgtataatga
     3481 gaaagtataa acttaatgtt tggaggagaa gatatgttag ggaaggtttt ggaagagtta
     3541 tttatccgga tctgggtcat tataaaacta accttgtatt tctggatata tacatttgcc
     3601 ggcgggatca tctttggatt aggtgcggca tggaaaacag tcaatgaact attttatttg
     3661 tacggattcg agtataaaga aatcacaata aaaagaggct ggaatatata taaaagaaat
     3721 tttttaagag gcaatctgct atttagttta tttttatctg gtactgcttt attgagttac
     3781 aatttatatt tatctgttca aatcaaaggg ctcatttttt tagctattga ttttatttta
     3841 ctttttgcct tgctctgcct atttgctact tatcaataca gtatgatttt agatagtgaa
     3901 tatacgatct ctgttcctaa tctgctaaag ctaagtttta tttcggtttt ttccagcttt
     3961 tcgtcttttc taaaaataat tattggtagc ggtataattt taggcgtgac ttggcaattc
     4021 aaaggattga tcctgtttgg tgtcattggt cttttgacgg tttggaatgg tacgatgaca
     4081 acacattggc gtgaagaatt agataaacag ttggaaagtt atgaataagt ttcggattct
     4141 attttcaaaa ggaaccttac taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg
     4201 tgtaacgatg actgctaccg ttatttttag tatccatact tgggaacaaa atcaaaaaca
     4261 ggcagaaaac atgacaagtg atgccgttca aagcacaagc agaatgctga atgataaaac
     4321 aaccttgagt aggatcatca aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt
     4381 gacgacctac ttaacaaaac ccatagatca gtatctgatg tatgtatacg aacagcaaaa
     4441 cagtaccgat gaattagttt ctttcccaaa tcaaatcaaa gatctctacg caaattatga
     4501 agaactaagt gcgatatata tcgtgttgaa tcaactaccg gagtattatg aatcttctag
     4561 agaaaacaaa ggtggggaaa taaaaaaggg gacaccaaaa ttactagacg ccttttatat
     4621 caagttgcct atcacacaag gaggagctga gtcaggaagt atttttattg gttttcaaaa
     4681 gaaagaacta gatatgattt tagaaaatct aacgtcattc aatggactct ctttatacat
     4741 gatttctgga acgacaaatc gactatacac attccatgat aataaaatag ctaaagaaac
     4801 gtttgaaaga caagaaatga tcatcacaga gagtctgaag aaaaattcac aattgccgat
     4861 tgacgatctg gaaaaaaaca attttgttca acaccaagaa ctgtctgatg attatcgtat
     4921 attagctgta ctagataaaa aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct
     4981 gatcattggt atcattattt tagacaatat actgcttatc tttctttata aaacattcaa
     5041 acgatattca cagcaagtat ctatcgtcat gcaggccatg gatcaaacag cagcagggaa
     5101 tttagagacg cggatcgaca taacaaacac ggaatatgaa ctgagggaac tttcaatcgg
     5161 tatcaacgaa atgttggata gcatcaatca gtttgtagaa gacatctaca aattagaaat
     5221 caaacagcaa gacgcacata tgcgggcgct tcaagcacag atcaatccac actttttata
     5281 taatacatta gaatatatcc gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga
     5341 tgttgtgtat gcgttttccg ctttgcttcg aaacaatacg aatcaagaga aaacgatcac
     5401 tttaaaagaa gaactggact tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc
     5461 taatcgagta gcctatcatt ttatgatcga tcctgatttg gaaaagatcg aagtacctaa
     5521 atttgtgatc cagccattag ttgaaaatta ttttaaacac ggtattgatt ttactcgatt
     5581 cgataatgcg ctaagtgtca aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa
     5641 agataatgga aaagggatga ctgaaaaacg gctaaaacag attgaagaaa aactgtctca
     5701 tcctaaagtg gaacttcacg gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc
     5761 cagcttcggc agcagttatt atatgtcgct tgagaataat gagacaggtg gactgaccgt
     5821 ttctattaca ttcaaggagg gatgagcgtg tatcaagtgt tattggtaga tgatgaatac
     5881 atgattttga atgggttaaa aaagataatt gattggcaaa gtttaggctt tcaaatagta
     5941 gcaacagcag agaatgccaa agaaggacta gctgtacttg aacagcgaca gattgattta
     6001 gtagtaacgg atgtgacaat gccagaaata aatggactgg aatttattga agcggctcaa
     6061 aaagaacgtc ataattttga atttatgatt ttatcaggat accaagaatt cgattatttg
     6121 aaaggaggta tgcaattagg cgccgtgaat tatttgatga aacccgtgaa taagttcgaa
     6181 ttggttgaaa gcctcaagaa aatcaaaaca cggttagatc agcaaaatga acaaaaaaat
     6241 caacaagaaa tctatcaaga actgttattc agtcaatggt tgaatgaaga attggatgaa
     6301 ataagtgaag aagaaataat aggacaaata agtgagaagc agcataggat agtattgatt
     6361 caactggcaa gaactcatga tactcttatc aatagatggt tgcaagaacg gcaacaaatc
     6421 ttttactatc aacgaaatta cggagaccgt gtattgatca cgttgttgtt ggaggcagat
     6481 gaagttgatg gcttctgttg ttttgttcaa gaaaactttc ctgatcagga atggctgatc
     6541 agtatcgggg aagagacaga ttctttggac aaaatccctg aaacattcca acaagcgaaa
     6601 gacaatctac agttgcatca attttatgga gataaagacg agcatgtttt ttacgcagta
     6661 caagcagaaa caaaagatca aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat
     6721 aaacaattgg aagtcgctca aaaaatgatc actgattttt ttgaacagtt tcagttagca
     6781 gccatgatgc cggaagatat acgatattcc agttttttac tgtttatgga gattcaacgg
     6841 gaattgattg atctggaaga tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca
     6901 aaaacggtgc aagaacttca gcagctactt ctgtcattca tcaaaaagca tcagagacag
     6961 aaaaagtaca gtgacaatgt ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa
     7021 ccacttacac tcaaagaagt gtcagaaaac ctgcatttaa atgtcatgta tctaggacag
     7081 ctgttcaaaa aagaaacgaa aaaaagtttt tcagcttatt tgaaccattt acgtatggaa
     7141 aaagcgaaac aactgctttt gcacagtaat caaaacatca atgagatagc aagtgagatt
     7201 ggctacaaca acaccacgta tttctctaaa ttattcaaga aaatcgtcgg cagatcccct
     7261 aaagagtacc gagaaaacag tggagaatca taaaagaaaa agccaatgta atgaaataac
     7321 ttataaaaaa ttgtctagaa atctaatttt gggctgttct tattacttga ggcaaggcag
     7381 ctttttcatg acctctcgat aaacgg
//
LOCUS       DAZKAS010000130         6924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000130 DAZKAS010000000
VERSION     DAZKAS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.132"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1248
                     /locus_tag="ACG8H7_002302"
     CDS             94..1248
                     /locus_tag="ACG8H7_002302"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFP8984373.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="ACG8H7_002303"
     CDS             complement(1270..2022)
                     /locus_tag="ACG8H7_002303"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8984374.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="ACG8H7_002304"
     CDS             complement(2386..5052)
                     /locus_tag="ACG8H7_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFP8984375.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="ACG8H7_002305"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="ACG8H7_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP8984376.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2044 a         1421 c         1252 g         2207 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaa
//
LOCUS       DAZKAS010000131         6859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000131 DAZKAS010000000
VERSION     DAZKAS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.133"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8H7_002306"
     CDS             complement(<1..182)
                     /locus_tag="ACG8H7_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFP8984377.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8H7_002307"
     CDS             517..951
                     /locus_tag="ACG8H7_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFP8984378.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8H7_002308"
     CDS             974..1294
                     /locus_tag="ACG8H7_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984379.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8H7_002309"
     CDS             1435..1626
                     /locus_tag="ACG8H7_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984380.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8H7_002310"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8H7_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFP8984381.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8H7_002311"
     CDS             2112..2375
                     /locus_tag="ACG8H7_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984382.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8H7_002312"
     CDS             2399..2587
                     /locus_tag="ACG8H7_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984383.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8H7_002313"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8H7_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984384.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8H7_002314"
     CDS             3070..3441
                     /locus_tag="ACG8H7_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP8984385.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8H7_002315"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8H7_002315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8H7_002316"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8H7_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984386.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8H7_002317"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8H7_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFP8984387.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8H7_002318"
     CDS             5250..5447
                     /locus_tag="ACG8H7_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984388.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8H7_002319"
     CDS             5553..6035
                     /locus_tag="ACG8H7_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984389.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8H7_002320"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8H7_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP8984390.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2327 a         1058 c         1348 g         2126 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgc
//
LOCUS       DAZKAS010000132         6731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000132 DAZKAS010000000
VERSION     DAZKAS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.134"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1062
                     /locus_tag="ACG8H7_002321"
     CDS             52..1062
                     /locus_tag="ACG8H7_002321"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8984391.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1131..2075)
                     /locus_tag="ACG8H7_002322"
     CDS             complement(1131..2075)
                     /locus_tag="ACG8H7_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP8984392.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2059..4779)
                     /locus_tag="ACG8H7_002323"
     CDS             complement(2059..4779)
                     /locus_tag="ACG8H7_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8984393.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4776..5996)
                     /locus_tag="ACG8H7_002324"
     CDS             complement(4776..5996)
                     /locus_tag="ACG8H7_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP8984394.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6277..>6731)
                     /locus_tag="ACG8H7_002325"
     CDS             complement(6277..>6731)
                     /locus_tag="ACG8H7_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFP8984395.1"
                     /translation="KELNYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQL
                     KANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSL
                     NDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         1984 a         1283 c         1093 g         2371 t
ORIGIN      
        1 ggtatagtta atatataaaa ataataacta attacaggag tgcaaatttt aatgaaaaaa
       61 aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc agcttgttca
      121 ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt atctgatttc
      181 tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat gatcatttat
      241 aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga tgcagaatac
      301 gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc tgctggatat
      361 acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc tggcttgaaa
      421 gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt ccatccagaa
      481 gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt gaaaaaatca
      541 gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga tacagagaca
      601 aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc tgcagaagta
      661 aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac tacaacaaat
      721 cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca aaataaaggc
      781 aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac taaactaagt
      841 gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa tgtaaaaatc
      901 aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc aagtacgaaa
      961 gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac tgattcaaca
     1021 aaagaatctt caactgaaga aacaactgat tcatcaaaat aaaagaaaac aaaaaaagcg
     1081 aagacttatg tctcgctttt tttgttttga atgaaaaaaa taaatttttg ctataaatct
     1141 tttggtacat agaaacttcg gttgtccata ccgaaaatcc gatcggtgta ttttcctggt
     1201 tctgtttgac gaatcgaagt caacatcatc tgcatcgaag catcgatatt gtcgatttga
     1261 tgtaatattt ctgcttccat tattcttgga cgtacaggtg agccatattc taataagcca
     1321 tggtgtgcta aaaccatatg acgtaataca agaacatctt cgtcagcttc gctgattttc
     1381 aacgcaagac aggctttagt tatttcttcg tcaatcaata ccagatgacc gatcaaattt
     1441 cctgcaagcg tatattctgt cgacatagga ccggaaagtt caatcacctt tcctaaatca
     1501 tgcaaaatga taccagcata caacaatgct cgattcagct caggatattc atcggcgatt
     1561 gatttcccta agtgaagcat tgtcaaagta tgataagcta atccactggc aaatgcatga
     1621 tggtttcttt ttgctgccgg aaactcaaaa aattgttttt gatatttatt aagtagatag
     1681 cgaacaatac gattccaatg cgcattggtg atctcaaaca acaattgatt gatttcttct
     1741 tccatttctt ctttcttcaa aggggctctt tccatgtata aactaggctc tcctggctca
     1801 tctacccttg ttaagcgcat atgaagaatc tttacttgag ggtttccttg ataaacctct
     1861 cttttaccgt ttagaaaaac gactgtgccg gctgtaaatt tattgatttc ctcctccgaa
     1921 gcatcccagt atttaccatc gatagtacct gaagtatctt ggaaagtaaa agcaataaat
     1981 ttttttccgt ttttagcgat tcttacatct gcacttttga tcaggacaaa ttgttcaaac
     2041 tgctcatcga ttactaaatc acgaattttt ttcattattt gctccttcta attgttttac
     2101 gatttggttc atctcatggt aatagcttac catttcttta tcagaagaaa aacaaattat
     2161 ttgatacttt tctccaaatt tagctaaaag ttcagctaat tgatactttc gctgatgatc
     2221 ataatgaagc cagccatcat caatgatgat cgggcatatc gaacggttag cttctaatga
     2281 gaggtaagca aatctcatcg ccataatcag ctgatctttc gtacctgtag ataactcata
     2341 gatgggaaat tctgtatatc cgtctgttaa agtcagtgta tcatttttca gtaatatctt
     2401 ctgataattt ccttttgtta aaatacctaa ataatcacct gctgctctta acaactgggg
     2461 taattgtctt tcactcatct ctgttgaaag atcacctaag aatgtcccgg cgatttcaag
     2521 tgctccccat tcctccagta attcctgtaa ttttgctttt tgccggctag cttgttgata
     2581 aagttcgtcc aatgtgccgt ctctttgcaa ctcttctact tgaagttgga gtcgttgccg
     2641 attttcactt gcttcttttt cttcttgctg gagttgggat tgcttttctt tcagttggcg
     2701 cagtcttaca gataatttct cttttgtcag ctcttcagga aatagtggag taagcatttc
     2761 cgataattca ttgtatcttt tcatctgctg ttcggcttgt ttggcttttt gtaaaaagac
     2821 aggtacttcc gaaggataag ataagcctgc ttgagtcaat tgttcgctgt attcttgtat
     2881 ccatactttt tgtttctttt tcaattgatt gatttcttgc ttcaataaag tattttgctg
     2941 atagcttctg gcaaatttca tctgttccat ctcttgtata aaacgggaaa gcaataccat
     3001 tttttctgaa attgtcttat cttgtatcgg taaccattct ttaacaaact caaatttttg
     3061 gtcgatttgt cgtagttttt cttgtatttc ctgttctctt ttatgtttcc gctcaaatac
     3121 gccatataat ttctgataat ctatgacttc ctgtccatat tctttcatga gtttgagcgt
     3181 atccatcttt cctaagtgcc gatgatccgc aaaacgatat acttcttgct gaagaatctg
     3241 tttttcttct ccagttctct tttgtttttc ttctgcttca gctaattgag ctaaggctag
     3301 atcaagttgt cctagttttt cttgccacat tccttttatc tcagagaaat catttttagt
     3361 tttaattttt ggttgccttt tgtagaagaa atagccaatc atgcctgcac caattagtaa
     3421 aaggatcact ttcaagggca ttggtacaaa tatacctgca atgattattg ctccgccagc
     3481 catcagcata tacggtttag atgaagagac tggtgctatc tctgactgat tttgtaataa
     3541 tgctggatag gtctcttcca agtcattcac ttctgcttca atcatctctt gctgttcttt
     3601 agctatttgg agacgcacag aaatctggct ttctagatca gcaatccttc tttcttcagc
     3661 cagaagatgc ttccattctt cttcatataa aaattcttcc ggtaacggat ttttctgcca
     3721 atgccacctt tgttccaaaa tcttcaattc tgaatttgtt tctctagtct caatttgtaa
     3781 tctttgccat tcttcgttta attgttccgc tgtaaattga agttgctgga gatcttttag
     3841 ttcctgctcc tgctctaaat aaaaataata tctggctgac tgctgatcca tcccgctatg
     3901 cttttccagt gttgcttcta attcttccaa acgctcattt gtttgctgat accgtttgga
     3961 gaaagactcc agatctaaaa gaagttgctc atcaggaaga cctgtagtgc tgtttttttt
     4021 gagagattgc cattcttcat ataaagaaaa attaagttct tgctgtctgg ctaatgaaaa
     4081 ttgtttgttc gcctctttta attgtgccaa taactgctgc tgttgcgact cgtattcctg
     4141 ttcttgttgg atcaacttgc caaaataagc ttcctgttct tgtttttgat gaattttttc
     4201 ttgcagctct ttccattcat ttaatttttg attgattagt aatttttggc cttttttctt
     4261 gaataattgt tgtgctttct gataatattc ttgccgcttt tggaatactt gagaactgcc
     4321 cgtgatgcct aaagaaatca gcgcatcatg tagctctttt tctctcaaat gatctagttc
     4381 catcaactgc tcttgctgga aagtaaatac ctgctggaat aactctttcg tcaaaggtgc
     4441 gatcagtttt tctagtaact catcacttcc cacttgatcc cccagccaca ccttcgattt
     4501 tccgcgattt tgctgcttgt agcgctcgac agcaaattca ccatatacag gatgtttaag
     4561 ccatattttg ccgccatagg ctgagccgtc ttttggtgta taatcctttt ttttacggcc
     4621 ttttgaaggg aaaccaaata atattgcttg aataaattga taaatcgttg attttcctgc
     4681 ttcgtttccc ccatagatca gctgattgcc tgaataaaag tcaaaacttt tttgacgcca
     4741 ttttccaaaa gcagcaatct cgatccttaa aatcttcatt cctgatcctc ctcaaataaa
     4801 aaatcttgat taagcagctg cttggctttt tcaagtgttt cttcttgata ttccggcagt
     4861 tcgctcaata ttctagccgc ttcttgatga ctatatactt catttaaaat ttgttggaaa
     4921 tcttgaggtg tctgatagta ttgaaataac tgttccatca atttagcaga agcagcaagt
     4981 ggggtcttcg tataaaaagt attctctatg agagaaatac gccaaataaa gaaatcattt
     5041 atcctatctg agaattcctc taacaaatag tctaatagct ctcctgattg tatccgttcc
     5101 aaaacatctg tattgagatg gtcataatct tttaatttta tctcaattaa actaaatcca
     5161 tcctctactt ttgaaagctg ctgacgaatg tgaacaatga cctcttttgt cgttcttgct
     5221 ttcctcaatg atatttcctt tgtcttccaa tagacctctg ctactttaat cggatgtatt
     5281 tggcaacttc ctttacttaa ttcaactagt agaatagatg tagaagcttg ttccttttta
     5341 gtatgacctt gaggcgcacc tggataaaca attgtttgct tttcatttaa gaccgttggc
     5401 acatggatat gccctaaagc ccaataatcg tatccctttt cttgcatctt tgagggttgg
     5461 aaaggagcat agtttccttt ttgagagacg ccaggttccc cgtggtacaa cccaatatgg
     5521 taatctgtga gttctctaga aggaaattcc atcactttgt ctttttgaat ccacggttga
     5581 cgataactaa atccactgag agagacagtt tctccacttt ttatcgttat tttttttgtc
     5641 tctaccgttt cggatgtaaa taaatggaca tttttaggaa aggagaacca ataccgctct
     5701 ttttgataga aatcatgatt tccgaatatt ataaatacag gtatatggtt cttttctaac
     5761 cgtttcatct gttctgaaaa atgtttctgt atttttaatg aaggacgatt ttgatgaaaa
     5821 ttatctcctg caagtaaaac aaaatcaacg ttatttatta ttgcctgttc aatgatattt
     5881 gataaaacgg tcagattcgt cttcaatagt ttttcctgta cagttttatc aagagttgtc
     5941 agtccttcaa atgaccgatc catatgtaaa tctgccgcat ggataaattt caccattaaa
     6001 aatttcctcc tttttttctt ttatcatacc attttcaatg ttttttttca tcaataaccg
     6061 aaaatttgtt cgtattatgc aaagaaatta cgataaatgg ccgacttcaa aaaataagtt
     6121 tattcgaaaa aaagattgtg acatgatttt tgtcacaatc ctaaatatct atgttatatt
     6181 cgcttcgttt gacactgaag aagtactatt tgtactggaa tttatgctcc atttttccgt
     6241 atttagaatt ctatgttcta tactacctta attagtttat atggtaagaa ttctttcaaa
     6301 tgcttgagat actccgtctt tatcatttga aacagtgaca taatcagctt ttttcatgat
     6361 ttcttgatga ctatttccca ttgcaaaact aatatctgca ctttcaaaca tagataagtc
     6421 atttaaacta tctccgatcg cgattctcaa aggtttcttt aaattgtaga ttgattccaa
     6481 acgatcaaac accacacctt tagatacatt ttgaggtacg atttcaatat ttacaggtgc
     6541 cgattcagta acagacacat ttagcatctt tttcaaatgg ttaaatatat tagctttcaa
     6601 ttgacgatta ggaataaaaa taaaaaattt gtaaatgctg atagcatcct tttctataat
     6661 ttcctcaata ttattataag ggattgaaca aatagatttt tcatactcaa gctcatagtt
     6721 tagctctttg a
//
LOCUS       DAZKAS010000133         6643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000133 DAZKAS010000000
VERSION     DAZKAS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.135"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8H7_002326"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8H7_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8H7_002327"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8H7_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP8984396.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8H7_002328"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8H7_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8H7_002329"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8H7_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFP8984397.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8H7_002330"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8H7_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8984398.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8H7_002331"
     CDS             3582..4271
                     /locus_tag="ACG8H7_002331"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP8984399.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8H7_002332"
     CDS             4285..5787
                     /locus_tag="ACG8H7_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8984400.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8H7_002333"
     CDS             5800..6276
                     /locus_tag="ACG8H7_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP8984401.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8H7_002334"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8H7_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAZKAS010000134         6630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000134 DAZKAS010000000
VERSION     DAZKAS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.136"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..2821
                     /locus_tag="ACG8H7_002335"
     CDS             146..2821
                     /locus_tag="ACG8H7_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP8984402.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3003..3242
                     /locus_tag="ACG8H7_002336"
     CDS             3003..3242
                     /locus_tag="ACG8H7_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984403.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3258..4631
                     /locus_tag="ACG8H7_002337"
     CDS             3258..4631
                     /locus_tag="ACG8H7_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8984404.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4635..6269
                     /locus_tag="ACG8H7_002338"
     CDS             4635..6269
                     /locus_tag="ACG8H7_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8984405.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6432..>6630
                     /locus_tag="ACG8H7_002339"
     CDS             6432..>6630
                     /locus_tag="ACG8H7_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984406.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2323 a         1071 c         1331 g         1905 t
ORIGIN      
        1 gtgatttcca aaatcacaac ttttttcttt tgtcaagatt ttgagtggga tatatttttg
       61 tagaactgaa gatcatgaaa ttatgagagg ttggaggtaa tgtagtcata caaataaaaa
      121 ttttttagaa agtgagtggt aattaatgaa aatgaatcac acttcaattc agaagcattt
      181 acaactgtta aatacactgg ggatttctta tgttaatttg agaacttttt ctacattaaa
      241 caagaaaacc gtttctcata ttttttctac tgcagattct caagcaatta ttaactattt
      301 agaaaaacag aaattgtctg aaggtacagt aaacataact tataatccta ttaaacaaga
      361 ggtgattaca caaaaaaagc attctgtgag agacaatgat attgaaaaaa ttcggtttgt
      421 atttattgat attgacccta aaaggaaaga gggaagtgca acagatagcg aaaagaaaaa
      481 ggcagaaaat gttatggaac aagtagaacg ttatttgaaa gaaaagagaa tagaatcatt
      541 tgtaaaagtt gatagtggga atggctacca tatttttctt cccataaatg aacaaccaaa
      601 taatcacgag acgattctta ctattcagaa ttttcttcaa ttacttcacc gaagatttgg
      661 agtagaagat gaagttgata ttgatacttc tgtttataat ccttcaagat tatgcaaatt
      721 agttggtaca ccagcggtca aaggaaagtc cacaaaagag cgtccgcatc gtttatctag
      781 tttcctttct attcccgata acatagatag aagtaattgt tttaaaattc ttgaacagat
      841 agttgaagaa aattccatga tgtcaacctc tagagtaaaa agccatccat cttttagaga
      901 agtgttacct tttattcaag cagactataa acaatggtta gctcactatc ctgaattaac
      961 ttataccata aaagagggcg atcataaagg agttacctta cttatatttg acgaatgtcc
     1021 attaagaaaa cacacgaata attctaatgg ttcttgtttg tctgttacgg ctgataataa
     1081 gattcgattt cattgtctgc atgcttcgga taaaaataaa gatattcaag actttgtaaa
     1141 aaaatatccg ttgccaaaac ctgtaaatcc ttctgtagaa tcattagaat ccaatcaaga
     1201 atattatttt gaccagttta aactgaatca agaaggagta tttttggtga aagaaggtca
     1261 agaagaaaaa gttgcttgtc cgatgtttat taagcaaata cgcaaaaata tcgaacaaaa
     1321 agtcattaca cttgttcttg cctattatgt cgatcattgt tgggatgaaa tagaaataga
     1381 aggtggatgt ttacaaacta ataattttaa acaattagca aaacatggct taatcattaa
     1441 agctagaaga gaagctgaag tcgttgactt tttacagatt caacgggaga atgctccaat
     1501 cttttttgaa catgaatctc ttggctggaa aaaagaaaat gaagcaatga tttttcagtt
     1561 acaagattct tttggacgtg aaattccttc tgtattaact aaaaactcat gctaccaatt
     1621 aacaactaat ggtacagaag cggaatttga cgcattggtt cataagtatg ttttaggaac
     1681 gaacttagaa cttgcttggt cgattggtat tgcttctatt gtgttaggct atttaaacgt
     1741 tacgaaggct aatacttttt tgagtttact aattaaccta cagggcaaga gcacaacggg
     1801 taaaaccact acgttacact ttattgcctc tttatttgga aatccaacta cggttcttag
     1861 aaatatgaat gctaccaata aagccattat aaagctagca tcaaacaatc agggaggtgt
     1921 tccactaata ctagatgagt taggagcagc aactcaaaat aactttagct cgttgatcta
     1981 tcaattagct tcaggagaag aaaggctaag attagattct aattgtcagc ttatgccaca
     2041 agagactttt tctgttttga cccttacctc ttctgaagag cgtttggaaa attatttcaa
     2101 tgagaaatta actggtttga aagttcgata tatcgaattt tctgatatca agtttacaaa
     2161 ggatgcgaat cacgcagaga aagttaaaag tctttcttct aatacttttg gttggggtat
     2221 aaagttattt cttttgaaac tctttgaaca agggattaca aaaattgaag caacttttgt
     2281 cgaagctcaa aaaaaactat gggctgtttt acctgaacat tctttgaagc aacgcattgg
     2341 taataatcta gctttgattt atagtggagc cctcctaatt caagatatac tgggctatcc
     2401 tttacgatta gagcatatac agacggtgct tattaattcg tacgaacagt taattgtaga
     2461 tactagttta ggaagtgagc aaacggtatt tgaccgagtg aaaacattgt tggtgatgaa
     2521 tagccataaa tttttaacaa acagtgacaa caaacatacg cccagtacga tttggggaaa
     2581 agtatcaatt caaaaaaatg gggatattaa ggtaaatatt tttcctatcc cgtttgagga
     2641 cttgttaagg cgagaatttc aagtaaagga tctaacctat ctttttaggc aattgcttga
     2701 ttcgggtaat atgtgtgcag aaaaagggag aagaacgaaa cgtattcaca tgaatcgtca
     2761 agttcttcca acatacgaga ttctattacc ttcttcactc aaatcgtatt ttcgattgta
     2821 atttagaata aaccaaagta tgaaagtgag gcaccatgtt atggaaatat ttcttattga
     2881 tttagcaaaa caactgatta cttatctgta tgatagcgag caaacccaaa cggaagtaaa
     2941 tgaagcttga tctatgcttc atttacttca ttcaatcaaa catgaaagaa aggaaaattc
     3001 taatgactaa aaataaaatg acactaaaag cagaggttct tctgtatatt caagaacatt
     3061 tttcaaatca agcctttttt acaaaaccaa tttacttgga tttcgaaata agaggggtat
     3121 ctgctggatc tatcgggggt actcttcaag cgttgaagaa cgaaggatat ttagaaaatc
     3181 actttgtgca acgttcgttc aatagaagag tcgtgaaaaa atggtattta gttcattcat
     3241 agagagggga atcaagcatg cgtgtggctt gttatgatcg aacgtctgtt tatgaagaag
     3301 cgaaaattag taatgacgct caatatgatg cagtgaaaga gtatgtttta gagaaatatg
     3361 aacaaattcc ccaacgattt tcagacaaag gagaatcagg ggaaaaagcg gagcgtccag
     3421 cttttcgaca gcttagagct tgggtttgtg cgtcagaaga agagggggtg atctatgttt
     3481 ggcgttatga tcgattggca cgtcaagcca aaatagcctt agaatttatt gagttgtgcc
     3541 aacaacatca ggttcaagtg tattcgattt ctgaacctct tcctgcttct ttctcacacg
     3601 cgtcaacgaa taaactattt gtgcaattat tgttcacttt tgcagaacaa caacgaaacg
     3661 tcattattga gaatgttcgt gccggacttt cctataaaaa gcaacaacgg caatatttgt
     3721 cttctgcggt accatatggg tatcgtttag tgaatgggaa aatccaacaa gaagtacacg
     3781 aagcaaggat tgttcgctat atttttcaac tttatttgac caaaaaatat ggctacaaaa
     3841 agctttgtca acgattgacg caacaaaagc ttttcttccg cgaacgtcct tttcagccct
     3901 atcatattta tagtatccta aaaaatccat tgtactatgg cgaaatcaaa ggaggatctt
     3961 tggggaagta tctaggaacc tttgaaccga ttctctcgaa agcaaccttt ttacaagccc
     4021 aagagattcg ccaatcaagg tgtacagcta aaaaagatac gtatctgtac ttattacgtc
     4081 aaaaaattcg gtgtcccttc tgtggtcgtc atctgagttc gaagtatcaa tggaatacaa
     4141 agaagacgaa gacgcttcat tattatcatt gtaccgaccg aagctgccaa ggaatttttc
     4201 ttcgggcaga aagggttgag tcagaagtat tggaaaagat acaggctttt tttcaatacg
     4261 aaggcgtcta tcaagccttg attgcccaat taacgcaaca attagccgaa aagcgaaaag
     4321 atgaaaaacg caaagtaatc caacaaaaga aacaagaagc acaatggatt aaggcatttg
     4381 aagagggcaa ttgttcactg gaagaactca agcaacaact gcaagctctt caaaagccga
     4441 tgtctacttc ttcgtttctt tcactccaac agtgtgaagc aaaagtgaaa gaatggcttc
     4501 aactaaaaga cctctctagt cgacaattat tatgtgacta tgtttgtcgg attgaggtaa
     4561 caaaggataa aacagtttgt gggatttact tccgacaact gacacaaaat attttagaaa
     4621 ggaacgaatg atctatggaa atgatacaaa ccaaacgagt cgcattgtat attcgcgtga
     4681 gtacacaaga acaagcttta gaaggacata gtattcaagc acaagaacaa aatggacgac
     4741 agtttgccga acggatgggc tatgaagtgg tagaagttta tgccgatgaa ggaatcagtg
     4801 ggaaatccac aaaaaatcgg ttcgcctatc aacgaatgat gaacgatgct cggaaccatc
     4861 gttttgatct actagtgatt tggaaattga cccgtctcgg acgcaatatg ttagatgtct
     4921 tacaaacagt cgaggaatta ttgcgatatg acgtaggatt acattcgatc tcggaacaat
     4981 ttgacgtgac cacttcgaca ggtaaattga tgttacaact ccttgggagt ttcggtgaat
     5041 ttgaacgcaa ccaaattgcg gagaatgttc aaatgtcaat gcaaagctta gtgaaagata
     5101 aaaagcgtta tgcaggaggc cgacgtttag gctatgtcag tggtattgac acgaatggtc
     5161 aaaaacaatt ggtcattgaa ccagaagagg cgaaaattgt tcgtctgatc tatgcaaaat
     5221 atttaggcgg agcagggtat cgagcgattg ccaatttttt gaatcgtcaa ggatatcaaa
     5281 cagtcaaaaa gaatgctttt tctaccacgg cagtcaaaga tatcttacgc aataaaattt
     5341 atgcagggta tttagaatat gcgcgctaca tcaattggga aaccaaacga cggaagggga
     5401 agaatccgag accgatttta gtgaaaggaa cccatgaacc gattatttcg gaagaagatt
     5461 atcaaaaagt acaagaacgc cttgctttag aaggaaaaca acccaaatgg aaccatacag
     5521 gtgaaaatgt attgacaggc ttgttacgtt gtccagaatg tggcgcaccg atggcagcga
     5581 gtaatgtcac aaatacccta aaggatggca caaagaaacg gattcgatat tattcttgtt
     5641 cagtctttcg gaataaagga gcaagtgtgt gtcatgccaa ttcgattcgt gccgaacaag
     5701 cggaaacatt tgtcgccgac cgattaaagg agatcgttca gctaccagaa gttcttcccc
     5761 gattggtggc agcgttaaat gaagaaatag tgcgacaaag ccaaccattg gaacaagaac
     5821 tcgtggtgtt actggaacga aaggaagaat tgaagactaa aattgaaaaa tgggaagctg
     5881 ctttggaaga tagtcctgaa ttgtttccga tgttaaaaga ccgcttagac gaattgactg
     5941 aaaaacgacg acagttgcac attcgagaaa atgaaatctt agggatcttt caacaaggag
     6001 agccgattca agtcaaagat gtccaacgga tattgactaa tttggatcgt tttcttgccc
     6061 agagtgagaa gaagcaaatc aaagccttgt atcgtacctt tatagagaaa ataacctttg
     6121 atcctaacca taaggagatg ttggaaatta cgatgaaatt cacgcatgcc gtggtacaac
     6181 aattgaatga gcaatatcaa atagccgtat ccaaaacaaa ggatacggct atttttgtgt
     6241 taaaaacacc ctttacgtta acgatctagt ctaggttata tcaaacaacg ttagaggggc
     6301 gctaacgctt ctctagcgat ttgaaatcgt tagagaagta gttagggagg tccttatcat
     6361 aaatagggat atttattaat gaatatttga aatataattg tatataagaa ttaaaaagag
     6421 tggggataga tatgaaaaag atgggtaagt gtattttatg tgaaaaatac actgaactta
     6481 ctaaagaaca tgtaccacct aaaaaatcag ggaatactgg aggtaaaaag accagaactg
     6541 gtaatttaga tgatttcctt aagagcgatt ttacaaaagg tgattttccg aaggggatta
     6601 aaaggaaacc tcaaggaaat gtttattata
//
LOCUS       DAZKAS010000135         6609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000135 DAZKAS010000000
VERSION     DAZKAS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.137"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..499
                     /locus_tag="ACG8H7_002340"
     CDS             185..499
                     /locus_tag="ACG8H7_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP8984407.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            520..897
                     /locus_tag="ACG8H7_002341"
     CDS             520..897
                     /locus_tag="ACG8H7_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFP8984408.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            907..1680
                     /locus_tag="ACG8H7_002342"
     CDS             907..1680
                     /locus_tag="ACG8H7_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984409.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1702..3105
                     /locus_tag="ACG8H7_002343"
     CDS             1702..3105
                     /locus_tag="ACG8H7_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFP8984410.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3287..4471
                     /gene="mobT"
                     /locus_tag="ACG8H7_002344"
     CDS             3287..4471
                     /gene="mobT"
                     /locus_tag="ACG8H7_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFP8984411.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4468..4758
                     /locus_tag="ACG8H7_002345"
     CDS             4468..4758
                     /locus_tag="ACG8H7_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984412.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4755..4976
                     /locus_tag="ACG8H7_002346"
     CDS             4755..4976
                     /locus_tag="ACG8H7_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984413.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5018..5797
                     /locus_tag="ACG8H7_002347"
     CDS             5018..5797
                     /locus_tag="ACG8H7_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP8984414.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5858..6358
                     /locus_tag="ACG8H7_002348"
     CDS             5858..6358
                     /locus_tag="ACG8H7_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP8984415.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6414..>6609
                     /locus_tag="ACG8H7_002349"
     CDS             6414..>6609
                     /locus_tag="ACG8H7_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFP8984416.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSD"
BASE COUNT         2269 a         1076 c         1312 g         1952 t
ORIGIN      
        1 gttgttttaa atatagtagg aatcggacgc tcagtacgga gcgtctattt ttttacccaa
       61 ttttgaaagg aagtgaactt atgaaaataa aaaatcaaga atcaaaaggt cgctctcccc
      121 cctttaaaac catcacacaa ataagattca ttcaaacata aaaacagaaa ggataggtaa
      181 aaatatggaa cttaaatatg tcattcccaa tatggaaaaa acatttggac aattagaata
      241 tgctggtgaa gggaacattg aacaacgtcg aataaatggc agaccaacca ttctctctcg
      301 tagttacaat ttatactcaa cgattcaacg agcagatgat attgttgtca ttcttccctc
      361 cgaagctggc gagaaacatt ttgaatccga cgaaaaagtc aaactcatca acccacatat
      421 taccgcagaa ggttataaaa ttggcaatcg aggatttacc aattacatta tgcacgcaga
      481 cgatatggtg aaagcataag aaaggaaagg tgaaaaaata tgagattagc agaaggtatt
      541 gtgattgaaa aagaccagac atttggcaca ttgaaatttt ccgccttgcg tcgtgaggtt
      601 cgtatccaga atgaagatgg tacggtttcc gaagaaatta aagaacgtac ctatgactta
      661 aaatcaaaag gacaaggacg catgatacaa gtcagtatcc ccgcttctgt tcctttgaaa
      721 gaatttgact acaacgcaga agtggaatta atcaatcctg tggcagatac cgtagcgacg
      781 gctacttttc gaggggcaga cgttgattgg tacgtgaaag cagacgatct tgtattaaaa
      841 ggacagacaa aacctcatca gaaatataaa aaagaagcgt ccgaaagtga aaaataggtg
      901 ttgcttatga caaacacacc attgattttg aaagaaattc cgaaggatga agccatttct
      961 tttattcggc aataccatta ttcaaagata ctccctagat tgtgtaaata ctttctaggg
     1021 attttttcag aagaaaaatt attaggcgtt gtggaattag gctggggtac gcaaccccta
     1081 caaaccatac gcaaactctt tcccgacagt tcccttcaaa ccacagatta tttagaaatt
     1141 ggaaagatgt gttttcttcc agaaatgaat caaaccaatt attttggcag tcaagctctt
     1201 tcagctctaa ttaagtggtt gaaagaacat acggactgtc attttttata tacactggca
     1261 gatggaattg agggaaaatg tggctatgtc tatcaagcgt ccaacttctt ttactgcggg
     1321 tatttcaaga caagtgtcta tcgtgataag cagtcatggg aaaagataca ccctcgaagt
     1381 gctcgccttt tattagagga aaatgctcgc tttgaacagg tagagaaaaa gcattggctt
     1441 tcccaggcat tttgcgaata caaagggatt gagaaaatca atggacgcat gtttcgctat
     1501 ctctatccgt tgacaaaaga agcgaaaaaa ctgttaggac acactctcta tcgacgtcat
     1561 tattatccga aagaaaaaaa tttacgcttt gaaaagcgaa tcgcttatca gaaatatgaa
     1621 gccatttctc aaccaacttt tgataaacag gctcgtatct acaatacaca attattttga
     1681 taggtggaaa ggaggatttt tatgccattg cttcatgaca gaggagagcg aatcaaagcc
     1741 aacgataagc acttggtcta tcattttgta ctaatttcat tgctggtagt ttttctagtc
     1801 gtcctactcc cttttcattg gagagcgtta caatcggtga actggcaagc agttcagtta
     1861 gatacatttt tctatcaact tcatacgcca tacttttgga gcagtattct cactgctctt
     1921 tttgtatgcg gacttatcag ctggctttat tatcactatc gtatagattc tataaaacag
     1981 ttagagcacc gtcaaaagtt agctcgtatg attttagaaa atcattggta tgaagccgaa
     2041 gaaactcaat cagagggatt ctttaaagat ttacccagca agaaaaccaa acaacgaatc
     2101 cgccattttc ctaaaatata ttatcgaatg aaagatggac taatccatat tcatgtagaa
     2161 atcaccttag ggaaatatca agatcagctt ttgcacctag aaaacaaact agaaacagga
     2221 ttgtattgtg aattagtgga aaaaacgttg aaagattctt atgtagagta tgtcctactt
     2281 tatgatacta ttggcaatcg aattggcatt gaagacgtgg tagctcaaga tggacgtgtc
     2341 aaattgatgg aatcggtcta ttgggagttc gattctctcc ctcacatgtt aattgctggg
     2401 ggcactggta gtggaaaatc ctattttatt ctcacattga ttgaatctct cctgcataca
     2461 aatgcaaagc tttatattct tgaccctaaa aatgctgatt tggcagactt aggggcggtc
     2521 atgaataatg tctattaccg taaagaagat atgttggcat gtatcaatca attttacgaa
     2581 gatatgattg atcgcagtga aaccatgaaa caacacccta actataaaac aggagaaaat
     2641 tatgcttatc ttggtttgtc tgccaatttt cttatttttg atgaatatgt ggcaatgatg
     2701 gatatgctag gtcgtgaaag taccaccgtt attcataaac tcaaacaaat tgtaatgtta
     2761 ggacgacaag caggcttctt tttaattctt gcctgtcaac gtccagacgc aaaatatttt
     2821 agtgatggga ttcgggacaa ctttaacttc cgtgtggctt tgggtcgtat gtctgaaatg
     2881 ggctttggta tgatgttcgg aagcgataca caaaaacaat ttttcttaaa acccatcaag
     2941 ggaagaggtt acgtggatac aggaaaaagt gtgattagtg agttttatac accccttgta
     3001 ccaaaaaggt acgatttctt aggagaaata ggaaaagtca ttcaaaaaaa gcaatctgag
     3061 cctgtaccgc acgaagtgaa aggtacaggt tcagacttgc tttgattggt gtggcgttag
     3121 ccacgccaat cctttagccc cctctatcta acaggggggc acaaatacaa ctgaaaacag
     3181 gcaattctca tcaaccaatg ttgatggtac aaaggattgc ggaaaatatg aaaggtgtaa
     3241 actcaagggt catagtttac atctttttta gattggaggt ttgcgaatgg aaaacgaaaa
     3301 aatttggatt aaggaattga aagaaaagcg actggcttat ggcgtttctc aaaataaact
     3361 ggcggttgct tctcatatta ccagacctta cctatcggat attgaaacag gcaaggcagt
     3421 ccctactcaa caggtgaaag aagatttact gaacgctttg gaacgcttca atcccgataa
     3481 cccattggag atgttattcg actatgtacg gattcgattc cctaccaatg atgtggctca
     3541 aattatcggt gaggttttac gtttgaatat ggattatatg ctacacgaag actttggcta
     3601 ttattcttat cccgaacact atcgtttcgg tgatattgtt gtcctagtct ctcatgatgt
     3661 aagtaaaggt gttttattag aactgaaagg aaaaggctgt cggcaatttg aaaactttct
     3721 attggctcaa catcggagct ggtatgattt ctttcaagat tgcatggaac ataaaggtat
     3781 tttcaaacgc ttggatttag ctatcaacga taaaacaggg attttgaata ttccagattt
     3841 ggcaaagaaa tgtaaacagg aagaatgcat ttccgtcttt cgaagcttta aaaattatcg
     3901 cagtggtgaa ttggttcacc gtgatgaaaa accatacatg ggaaacacac tttatattgg
     3961 ctcattaaaa agtgaagtct atttctgtat atatgagaaa gattatgagc aatacgtgaa
     4021 aaatgatgtt cccttagaag acgcagaagt aaagaatcgg tttgaaattc ggttaaaaaa
     4081 tgaccgagcc acccaagcca tgaaagattt attggcacat caacaggcgg aaaaaacagc
     4141 ctttgaaatt atcaatcgct atatccgctt tgcagataaa gacgacacta aacgtcggag
     4201 tgattggaaa actaatgaac gttgggaatg gtttatcggt aaaaatcgtg gagcattacg
     4261 cctaaccaca cagccagagc cttattcttt tgaacgaaca ttgaactggt tacatcatca
     4321 agtcgcccct acattaaaaa tcgcaagcat tttagatgtt ctcaacggta caaccattat
     4381 ctccaccatg attcaggaag ccaaactgac agaaaaacat gagaaattaa ttgaacaaca
     4441 acatctagcc atggaggatt tgattacatg agccttttaa caaaagaatt aaagaagtta
     4501 gggtttcagt gtggcattga gtttcaagcg tatatacaaa atacagggaa atatacgtct
     4561 ctcattattg agggaaaacg gcaagctggt gataccatct atacctatga tttttacaag
     4621 gtgagatttt ataataatta taccaatcga gtaaccgtct atggagagca tttaacccct
     4681 tttcaattat taaggcgtgt aaaaagctat atctattatc gagaaaaata tcttaaggaa
     4741 aggaggacaa tcacatgaat tttgggcaga atttgtacaa ctggttcttg gataatgcac
     4801 agtcattggt attgttagcg attgttgtca ttggactgtt tttaggtttt aaacgagaat
     4861 tttcaaaact cattggcttc ttgattattg ctctaatcgc cgtcggactt gtgttcaatg
     4921 cagctggcgt gaaagatgtg ttattaaact tatttaaccg aatcattggt gcttaacaga
     4981 tatttttcat gataaaatag gaagtatata ttttgttatg aaaggattta tgaatatgtt
     5041 tacaaatgat caatttaaat ctcttttaat taaaacatta gaaagaaaga gctcttcaaa
     5101 ttattatgaa ggaggtaacg agattgtttc taaaatgaaa atatcagagg taactttaga
     5161 tgagactgac gattttactt attataaagg atataaaaag ggctggaata cgctttgcac
     5221 atatcttaaa ataagagttc cttttgatga tttagacttt tttgaaagtc ataaagattt
     5281 gattacccag acagcaagct ccatatatga taaacaaggt gataatgtat tagtggatac
     5341 tattttagta ccacttccag agaattacga agtaattaac tttagtcagt tgaaaattag
     5401 tgatgtggtt tcacaagcta ttgaagacgc tgagtctttt atgagtaatg gtgaatatca
     5461 acgtgccttt gacagagttc acactgcttt tcatggatat ttaattgaaa tactaaaaaa
     5521 atatgaaatt acagttccta gagacgaaaa tctatctaaa ttatatagta ggatacaaca
     5581 attaattgaa aaagaaattc aacctactga acttgctgat atagtgaaga caacgataag
     5641 gtcatcaaat ggaatgatta gctctcttaa tgaagcaaga aataggcatt cacttgcaca
     5701 ccctaataca aatattattg gaaagagaga agcaaaacta ataataggta tttcaagtac
     5761 agttacagat tatattagcg gataccttga taagtagcgt cattaactga ttgctacttt
     5821 ttttatgact atttttataa aaaggatgtg ataagaattg gaaatgaaag tctatgtggc
     5881 aaatttagga cggtataatg aaggtgaatt agtcggagca tggtttactc caccaattga
     5941 tgaggaagaa atggcagaaa gaatcggttt aaatgaggac tacgaagaat atgcaataca
     6001 tgattttgag cttccttttg atgtggacga atacacgccg attagtgaaa tcaaccgtct
     6061 atgtgaagcc attcaagaaa tcgaaggtac acctatttat aatgaactca aagaaattca
     6121 aggcatgtgg tttagtagct tggaagaatt actggaaaac aaagaggata ttcattgtta
     6181 tagtgattgt gattcgatgg aagatgtcgc ccgttattac gtggaagaaa caggacagct
     6241 tggtgaagtc ccttccaatt tacaaaatta tattgactac caatctttgg gacgtgatat
     6301 ggaaatagag ggtaattacc tagttacctc tcatggggta tttgaatatt gtcagtaagt
     6361 ttaacaatcc gtgttataat taaattaata aatagcacgg agaggtataa attatggtaa
     6421 atgtatttga aaatcttaaa ctattaaaac tagacatgaa agataaaggc tgggtgatag
     6481 attcctttta ctttcgttac aaacagcaaa attatattgt acttgttaag ctatttgaaa
     6541 aagaggaaaa agttccagaa tatgctctgt taaaactaga atttttaaag gaaaacgatt
     6601 tttcagata
//
LOCUS       DAZKAS010000136         6494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000136 DAZKAS010000000
VERSION     DAZKAS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.138"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..503
                     /locus_tag="ACG8H7_002350"
     CDS             51..503
                     /locus_tag="ACG8H7_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984417.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            517..1206
                     /locus_tag="ACG8H7_002351"
     CDS             517..1206
                     /locus_tag="ACG8H7_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984418.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1331..3682
                     /locus_tag="ACG8H7_002352"
     CDS             1331..3682
                     /locus_tag="ACG8H7_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFP8984419.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3762..4157
                     /locus_tag="ACG8H7_002353"
     CDS             3762..4157
                     /locus_tag="ACG8H7_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984420.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4482..5099
                     /locus_tag="ACG8H7_002354"
     CDS             4482..5099
                     /locus_tag="ACG8H7_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8984421.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5140..5439
                     /locus_tag="ACG8H7_002355"
     CDS             5140..5439
                     /locus_tag="ACG8H7_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFP8984422.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5473..5640
                     /locus_tag="ACG8H7_002356"
     CDS             5473..5640
                     /locus_tag="ACG8H7_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP8984423.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5685..6290)
                     /locus_tag="ACG8H7_002357"
     CDS             complement(5685..6290)
                     /locus_tag="ACG8H7_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8984424.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2243 a          896 c         1279 g         2076 t
ORIGIN      
        1 cttatcgtag atatatatta taaatttaat aagaaagcga ggtgagaatc gtgaaaaaaa
       61 cgatactaag tattttttca gtatttacaa tattaattgg gggctatttt cttttgaatt
      121 tagattctgt taattcaaac gctcaaataa aagatcaaag agtagctgaa cataataata
      181 aaaaaatgag tttgaccaaa aatatctatt ctatgtcaga tgaagaattg tataaagaat
      241 ttggtgattt atccggggaa cagttagaag aaagtattcc aggaattagt gactcagata
      301 aaattgttgt cttgtctgct ggtgggcaat taatgggaga tggcaaatcc gaattagtta
      361 gttataaaca aaatcacaga cagtttattg atgttaatac tgattccaac gctatttcga
      421 ttaaggatct aaaagagctt ttaaatagta aaagaaatga attaaagcag ataaaagaaa
      481 ctaattcagt ggaattagag taaaggaaga ttcattatga aaaagatttt tggtgtttct
      541 gttgggctac ttatgatgtt aacatttgga aatgcagtat atgctgctga gaaagaagtt
      601 atttcagatc cagagcagta cgcctcacga tactttgctg attttgaaga agaagataaa
      661 gtacttgcat tgccagaagg tggtttttta aaaggggaag ctaaaattgt atctatggat
      721 aatccagata taattcaagg ttcatataat tcagaaactg atcctgcttc agtttcagtg
      781 acagaagcaa aaaaaattgt tgctgaaaaa ttcaaaaatg atattgaaac tactccaatt
      841 atgcctcgag gagcatttcc acccgatgtt ggtactagcg gacaccttct tgtactagca
      901 ggaggaggaa gctataaatc acagccattt agtggtgcgg gatggcgttt tggaggtatg
      961 ggtgtaaaac cagctgatgg tacaggcaac tttttacgtt ggaaatccta cattgacgat
     1021 ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat ctggttctat tcaaggaatg
     1081 gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa acgggcctgg ttggacaatg
     1141 aatataggta tttattacac ttataatcct gttgcaggta cgtattatgt tgttcaaaat
     1201 atttaataaa acagcacaaa gagatttctt tgtgctgttt tattattttt agttgttgac
     1261 aaacactaaa atataaaata taatcaacac ataaacgaac gtatgttcgg caagaaagca
     1321 ggggattttt atggatgaga tggttttaag tactcagaaa tggctaaaca agaaatacag
     1381 taatgttaca gggtttgata aggttccaga aaatgggcga acagggtggc ctacaatcta
     1441 tggacttatt gaaggactac aagtagaact tggtattact aatctagtag ctaattttgg
     1501 acctaccact gaaaaaatgt acgataatca ggttactcct aaatggggca aaaatctacc
     1561 taaaaatatt gtatttctta ttcaaggtgc cttttggtgt aaggggatta atccaggtgg
     1621 cttcgatggg gtatatactc catatttaga tactgctgta aaagagttgc agactgatgc
     1681 agggtttaat ggaagcgctg taactggagt tttggatgcg aaatgggcca aagcgttatt
     1741 tgatatgagt gcttttgttc ttgttcccgg cggagatagt aaaattagaa aaatgcagca
     1801 atggttaaat gtaaattatt tagaatacac gggaataatg ccttgtgatg gtatttatca
     1861 aagaaattca aatcaagcat taatttttgc attacaagct gaactaggat attctcccag
     1921 tgaagcaaca gggtcttatg gtaatgggac aactagtaaa acttatcctg tgtctgaagg
     1981 taactcggga aactatgtaa gaataattca atatggatta tatgttaatg gttattttga
     2041 agacggtaca ttcgatggta ttttcactaa atatatgggt ttagaagtac tggcattccg
     2101 aaaatttatg gtattgcctg aatatactga aatagcagat gttgttgtaa taaaaggact
     2161 attgtctagc gctggagata ttagaagatc tgcagatggc gcagatactt ctacgcaatt
     2221 aactagatct caaatccaaa cattggttga taatgatata aaaatagttg gtagatatct
     2281 tactggtact gttggaattg gaaaagatga acgaaataag tatctaacta cagaggaatt
     2341 gaataatatt ttttctaaaa atttatctgt gtttcctatc tatcaagatg gaggtgcagc
     2401 attagcttat tttacttatg atcgtggctt atcagatggt aagaaggcta tagatgcagc
     2461 aaaaaatcta aatataccat taacaacagt tatttatttt gctgttgatc tagacatgtt
     2521 aggggaagaa attcttgcat atgctggaga atattttaag ggggtatctg ctgtaatgtc
     2581 atatagcggg tatcaaacgg gtgtttacgg aacaagaaat gtttgttctc aaataatcaa
     2641 taacgggtat gcttcgtttg catttgtttc agatatgtcg actggttata gtgggaattt
     2701 aggttttcct atgcctagac aatggtcttt tgatcaattt gttgaattta ccataggaag
     2761 tggcaacgga gctgttggta tcgatttaat cgctacatcg ggtagagata gcggatttaa
     2821 tgagttatca aatgatacta ataatgataa ttacattgcc aaatacaatc agaaggttat
     2881 aaatcagatg gtacgtgcgt atcaatatct atcacaagct ggtcttgata atccttggaa
     2941 ccctcatttg acattttata gatatgtaaa ttattctggt ttgtcatggg acatcatttc
     3001 ttctcctgta acagagcatg atagaaaaat ttatgatgaa tataggaata aattaactaa
     3061 tagcgagggc ctgtataatt attttataga tcctaatact ggttcaataa taggtttgcc
     3121 tcacttaatt gtgacgttac aatcacagat gtttctagcc gacacaatca atgatagtgt
     3181 ttctgacttt gcaggttggt taggagattt aatgacttgc tggggtgaag ttaagaaatt
     3241 aggaatacaa atggagcaag gtgtatttgc attagtaggt acggctgcag gtggaacatt
     3301 tagtctagaa gatttatacc aagatgttga cgcggtaaat gtttatacct tagcaaaaag
     3361 aaattctgat tcaacagctt tatcggcttt aaaaacgtac tattgtgata agggtgctat
     3421 tgatagattt aatcaatttg ttaagaacag atttggttct tcagataaaa tcgcttcagt
     3481 aacaaatagt ttattagaaa atgacgaaac agttgatatc ttaggtgtag ggataatttc
     3541 atttttcatt gataaaatag ttaataattc acttgggaca gattttactt atcttgattt
     3601 tattggagat gaggggaaag ttgcaaatgg attttctgaa aaaattattc aatttcaaga
     3661 aaatagaagt aaacttaatt aagaatcgtt tactatttaa acagtgtcct tttccttttt
     3721 aaatatatac gataaaataa gtatatgaat tgtaaaagga catgatgatt atggaagaaa
     3781 tagaatggaa taatctgact caaaataata aaaataattc tggatgtgga attatcttta
     3841 tgtttgttat agttttggga atagtagtac ttttaataac tgtcaagata gtagattact
     3901 ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc tgagtttgaa agagtaccac
     3961 cttatgatcc aagagataat tcatttcaag taaaaaaatt aaaagagtct ccaaatgaaa
     4021 tgtggtttga agttacagtt gaatcaagcg acttcaaaga aaaaaagaaa gaatacaatt
     4081 atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa ccctaaatta gaaatatata
     4141 gtgttgtaat agaataatag tgttaaaaaa attgtaaaag ttaaagaagt caaattctga
     4201 tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa actggataac tagtttattt
     4261 aatggcagac cgttaaggcc tgccattttt atgtatttta gcggaataaa tataatcatg
     4321 cgggagtgta aacttactgt ttcaaaaagt atactttttg acatgccttg attttctttg
     4381 tttatgtatg gcataataac cacataatac aatttgaaac gtttctgatt taatctgatt
     4441 gttttgaaac aattttagtg aaaaaagggt agataaaaac gatgagaaaa tttttttata
     4501 tccgggtcag tagttatgat caatcgatcg aacgacaact gatttccgcg aaggaattag
     4561 gaatccctga agaatatata tttgtagaaa aagccagcgg caaagatttt aaacgccctg
     4621 aatatcaatt aatgaaacgg atgtttcgaa aaggagattt actctatcta caatcattag
     4681 atagactagg cagaaataaa caaatgattt tagaagaatg gcgagaatta actaaggaaa
     4741 agcagattga tatttctgtg ttagacatgc ctctgttaga tactgctagt tacaagcaac
     4801 tagatgggtt agagacgctt atctctgact taattctgca attactcagc tatatggccg
     4861 aagacgagcg gaaacgaatt aatagccggc aaagagaagg cgtagaagct gcgaaacaaa
     4921 agggtgtaaa attcggcaga aagaaaattg ctttacctcc tgaatttcct gcgatctatt
     4981 ctgactggaa agcaggaaag ataactgcag tctatgctat ggatcaattg ggcatgaaac
     5041 gaaatacctt ttatcgacgg gtgaaagaat atgaacatac cttgcaccac ttttcttgat
     5101 agatataaca gctattagac gggaagggag agtttgaaaa tggacttaac gaatcaaagg
     5161 gtcgttttta ctggaacgct gcagcaaatg acacgaaccc aagccatcgg tttggttcac
     5221 tctttaaacg gccattgtca atcaagtgtt acttcaaaaa caaatttcct ggtttgtggt
     5281 cagtattcta agtcgttgtt tgatgattct ctctatacaa aaaaggaaca aaccgcgcgg
     5341 gacttgattg cgttaggtca tgagattacc attttatcag aagtcgaatt ttatgcgtta
     5401 attagtcaac agttgaaaga atggcaacgg tatttataat cgttagtaaa atttacaagg
     5461 aggaaaattt ttatgaagcg aaaatcttat gatcgttggg gcattgaaat aactagtttt
     5521 aatcgaaata ttgcttttca cgtggagtgt gactgtgggg aattagcaaa aagtgtttat
     5581 caaaaaaatt acttttactg tcctaaatgc catcgccgat acggggtaca tggaacatag
     5641 aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat atttctacaa atcattcgct
     5701 ttctttattt tataaatagt tggtcgagag atcccgtttt cttttgcaat ctctgcaact
     5761 ggaatgcctt gctttaacat gtccaccacg gttttaaaaa ctaagcgttt ttgcggatct
     5821 gttgagttaa tagaatactc gattggtcgt cccttatatc ggccggcttt ttgagccaat
     5881 aatactcctt gccgctgacg ttcacgaatt ttctcacgct cgttttgtgc aatataactt
     5941 agcagtgata aaaacatatc gaacatagcg gtatccagga gttcatttcc agtattaaaa
     6001 tctaaaaact gtgcatctaa tatttttaac gctacttttt tctgtttcat aaaagcaact
     6061 gtatttttta tatcttcata atctcttcct aaacgatcta gtgatgtaac gatgacgcaa
     6121 tcaccttcac ggatataatt tagtaatttt tgaaactcag gacgctcagt atctttacca
     6181 ctggcttttt cttggaataa tttatcaatg ttaagtcctt ccatcattgc caattgtcga
     6241 tccaaatttt gatcttgtga agatactcga atatagccaa tttttcccat aaaaaactcc
     6301 tttttaaaag tgatataatt tacatgtaaa atttacttgt agttttactg ctatcatagc
     6361 atttttaaaa ataaatttta tgtagactta aaatttactt catggagggt ttaaatggat
     6421 gaaatcaaaa gtaacaaaaa agcatttggc atttatttag gagaacctag tacagaacaa
     6481 ttaaaccaat tttt
//
LOCUS       DAZKAS010000137         6420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000137 DAZKAS010000000
VERSION     DAZKAS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.139"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..249)
                     /locus_tag="ACG8H7_002358"
     CDS             complement(4..249)
                     /locus_tag="ACG8H7_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984425.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ACG8H7_002359"
     CDS             complement(266..1117)
                     /locus_tag="ACG8H7_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP8984426.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ACG8H7_002360"
     CDS             1283..2287
                     /locus_tag="ACG8H7_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFP8984427.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(3070..3627)
                     /locus_tag="ACG8H7_002361"
     CDS             complement(3070..3627)
                     /locus_tag="ACG8H7_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984428.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(3806..4417)
                     /locus_tag="ACG8H7_002362"
     CDS             complement(3806..4417)
                     /locus_tag="ACG8H7_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984429.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(4530..4787)
                     /locus_tag="ACG8H7_002363"
     CDS             complement(4530..4787)
                     /locus_tag="ACG8H7_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984430.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(5384..5662)
                     /locus_tag="ACG8H7_002364"
     CDS             complement(5384..5662)
                     /locus_tag="ACG8H7_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984431.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(5696..5947)
                     /locus_tag="ACG8H7_002365"
     CDS             complement(5696..5947)
                     /locus_tag="ACG8H7_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984432.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1990 a         1231 c         1021 g         2178 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa
     2401 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc
     2461 ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa atggtgtcca
     2521 aaagcttgca tctgccagat tatctcattt cttatgacaa gctaagcaaa ttatctccac
     2581 caaataattt attcatattt ttgacttgcg catactaact aattcttaaa ttttcataca
     2641 aaatttctca atttccctta acgagtaata tttatgtacg gtgattctca ctagtatccc
     2701 aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc ttttatgtat
     2761 gctgttagtt gattagatgg tcatttgttg agctaggtcg tttattttaa acaagttgtt
     2821 tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa atgcatacgc
     2881 atctgtattt gccactttga ttcgtataaa gctaacaact attaactcct ctcagctaat
     2941 agttgctctc ttttttattc agattcattt ttgctgatca cagcctagta tttcaatggt
     3001 tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa atggattagt
     3061 agatttcgat tattcaaagc gattttctat tggtggatcg tgttcccaac taataagacg
     3121 gattccaaac ctatcgtaca gatcttctaa accacttata aattttgcac ctatttcagg
     3181 tgttttattg cgtggatatt ctattgttac gccattaaat ggataccaat taggattagg
     3241 ctctcttttt ttctgtacat ctcccataga atagttagca ctttctggga cataatatat
     3301 tcttcgacca tcctctaatt catctaataa gatatttcgt gatcgtctat cacttgctaa
     3361 atgtagacgc cacgtgcgat accatgccat gctacctgca tctgcagaaa atccatcaaa
     3421 taatgcaaca attgtatctt ctatcacaat cctttcccca tctacttcat attctaatct
     3481 aaaatcaaac tcccctcggg taatttctgg tcttggtggc ttaggaccca atattattct
     3541 acttaaaacc gcattcgttc ccagatgaaa cacagctata acaacaataa tgattaatat
     3601 tttgtttcta tttgataaat ttctcattat tcgcacatca cctctacatt ctcacatatt
     3661 agttcgtaat actcctttaa tatcaaaata tctactggtc gtaataatat tcgctatgaa
     3721 ttcctactac tcgtataaga aaggccaaat catcagcttt tacttgctga tgatttggct
     3781 tgtttctatt tatcgtaatt gttttctaat caccaaaatt gttaacaatc ggttcagata
     3841 tttcccagtt gattaaggta attccgtatt gtgtcaaaac ttcttgcgct tctccgaaat
     3901 taagtacagt tcttatgtat ccactttctc ctaatataaa gtctacctct cttttaaaac
     3961 cttcctcgag atttctaagg gctatatgtg gttttaatga cattgattgt acattttccc
     4021 ccatataata ttgcgcactt cctcgactaa aatagattcc tagatcgtcc ccaaaaggta
     4081 gatcttctct accactagct aatctacttc tccatcttcg ttctcttttc atagaacctg
     4141 cgctgaactc acacccgtta aactcagcta tgacagtatc ttcaattatg ttaatctgtc
     4201 catctagttc gtattcaatc ctaaatggaa attctccata ggtgatttcc ggttcgggta
     4261 ttttaggtga gattaatgca aagaaaggaa atattaagct tggcaacatc aaaaaaactc
     4321 ctcctatgat cacaattccc gatgtaatcc aaaatataat ttgtacaatt ctcattgatc
     4381 cattgtattc cattttattc cccctattac tattcatatc cgcctatcta tatcgatctt
     4441 ccaatgcaga gataattgct tccatacctt gattttttac tgagttacct atgcgagata
     4501 tcattctcca ctaaggtgca tcatattgtc tatctaacat gattaattct ccaataggtg
     4561 tatactcctc caaaaacaac atttaatgca tctccctcga caacataagc taccatgttt
     4621 gctgtatatg tgtttgagtc taattcataa gcagataaat ttactgtcgc tggattaaat
     4681 ataatcgcat tcgtatttat agctactgaa tttgctgctt cagcaccacc ttttgaatgt
     4741 ccgatcattg tgatttcaat agtaggattt acgtggacag ccctcaccgt ttaccctgac
     4801 cattatagaa atcgcttact tttttgaact acgcaacaaa ggatacaatg ctcctaactt
     4861 cactgtctcc atttttgtcc actaaggaat ataaaaaata ggcattaatt catttaattc
     4921 ggtcacaaca cgttaccttt taaatcatta tactgtatct catacattat tatcaataaa
     4981 ctattataaa aatgcaaaaa atgctttatc aatctatttt gtaattggtc ataatatcat
     5041 tgaaatcata ccctgtatcg atttttccta tgaatctcgt ctatttgtga ttgacgaaaa
     5101 agaggcaaga cagacactgg aatggtaatt ttacccacta ttttgctcca actatttgta
     5161 atcgtaccca cttttgaaat accgagatga tcattttaat tgatatgctg attcccaagc
     5221 aaaaccccaa gaaattaaat gctctatcgc atctagcttc ttgggttttg cttatataaa
     5281 ttccaacatt taaatttttt atgctgttcg gtctcagaaa tgccgttttt gcttatatca
     5341 caatatcaag atgtcaaatt attcgtgttg ccgatgactt tgattaattt ttttcaaatt
     5401 gtttattaag actaatgctg tctttttgca gataccaatg ttcgaacttt ttaacattat
     5461 catatgatcc tgcaagtttt aacttcttac cattagatgt tatcaaagtt ataattaacc
     5521 ttgccttttt cccattagga tcttttattt tatgaatttt ctgcaggtaa tctctcctaa
     5581 tggcagctat ttttacctca tcagtgctga taaaccattg atcatccaca tacatactgc
     5641 ctgttatgtt gtgtttttcc atttcttccg agaacgaaaa gtttaaggct cgttcttact
     5701 tccaaagttg aatgagtcca tggattggac ttagtgtgag aattgagaac aacaataaga
     5761 atccatatcc tcccgcaaac atccctaagg ctatactgtc tctccatcca taagtaggat
     5821 tagaagagaa aagagtcacc gtttgtaata atatgaaaag gaataatcca gcacaaacac
     5881 caaagataac aactccaaac attgaatttt tatacaatga ccatttattt ataggctttt
     5941 ctttcatttt acttccccct actgttctgt tcacacggaa tgaattcaaa gttcataagc
     6001 cctcctcttt gaacatatga atacgacttg ctaactatac tttaaaaaag cttgttttaa
     6061 gcggttatag gcgagttgta atctctattc atctcattag caaataactt gccgaaaagt
     6121 ctactgaaat taaagcagat agtggaagaa ttatttagcc aggatagaca ctatttgtga
     6181 aacacatgtc tttacttttg aaattctatg atgaaaagcc ttggaataca gtagctctta
     6241 attggtaata ctttttccat tataaataac ctcttttcat tttatattta tcccctctaa
     6301 aaataatatc taatctaata ttttaaactt atatttccct cgctgtgcta atagagcatt
     6361 actaaataaa tagaaactag aaaaaagcat tacgcctgcc agaataaacc aattatttgt
//
LOCUS       DAZKAS010000138         6331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000138 DAZKAS010000000
VERSION     DAZKAS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.140"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1305)
                     /locus_tag="ACG8H7_002366"
     CDS             complement(<1..1305)
                     /locus_tag="ACG8H7_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8984433.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKA"
     gene            complement(1464..1904)
                     /locus_tag="ACG8H7_002367"
     CDS             complement(1464..1904)
                     /locus_tag="ACG8H7_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP8984434.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2064..2570
                     /locus_tag="ACG8H7_002368"
     CDS             2064..2570
                     /locus_tag="ACG8H7_002368"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFP8984435.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2738..4651
                     /locus_tag="ACG8H7_002369"
     CDS             2738..4651
                     /locus_tag="ACG8H7_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8984436.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4656..5657
                     /locus_tag="ACG8H7_002370"
     CDS             4656..5657
                     /locus_tag="ACG8H7_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8984437.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5859..6065)
                     /locus_tag="ACG8H7_002371"
     CDS             complement(5859..6065)
                     /locus_tag="ACG8H7_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFP8984438.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2198 a         1031 c         1310 g         1792 t
ORIGIN      
        1 ggctttagtc agttgggtag tgttcttagt tttcttgaaa tattttcctc gatataaaaa
       61 gattcgttct cggtagtttt gaaagtttct aaagccatag gcaattcgtt ttagtacttt
      121 gatatggttg ttcaaacatt ctatagggcc gttggaatag ggaactcgca acgctcgttt
      181 gatttctgtt cgatatttct tgaaggtcgt gaacactggt tgatagtgct ctggcaactc
      241 cttgtagtct tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc tgatagcgga
      301 aagaaaaacc tggtagactt cgtagcctcg ctttaaggag tcatcataat ataatagccg
      361 atcgactatc tccgcttctg tcagatgatc tcgaaatgac ggacgccagt agcgatgctg
      421 accattcaat tcccacgcat tcttttgaag tagtctccag tatttcttta agtgcttcga
      481 ccgacgatcc ccttttttaa aggtcttcat tacttgaatt cggtggttgg taaaggcccg
      541 accgatgtgt tggctaatat gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc
      601 tttcaccaat gtgcgatagg gtgtatagaa atctgaaacc actagacgta cttgttcacg
      661 gacttttcta gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc gattttccac
      721 aatatcaatc agttgatggg tcttgccgtc catcatgaca aagctcatgg ctcccgagac
      781 gtttttcacc gatttgaatt catcgaaaca caccacttct ggtaacggct ggttcaaaga
      841 aacggtttta ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt
      901 catacgtgca atcaaagaca tagaaatagg ctcacttaat agctcaagaa tggcttgttt
      961 gactctgcgg gcaatggagc agcgacgatc gctaactgaa tcttctgcta aaaaagtctt
     1021 tccacagtgt ttacatttga agcgctgttt gttaatccgc acaatggtct tgtagttact
     1081 gacatcattc aataagatgg atactttttt ccagccccat ttaatgatcg tttgcttatt
     1141 ttgatagtgg caatggaagc attcctctgg ttgataggtc aatgtgccat caaaaacaaa
     1201 acatgtttcg tcattaatgc gctctttgcg aaaggcatct tcatgaaagg taagatttag
     1261 gtctagaata tctagtaact ctttggtaaa ctctgaatag gacatcttga aacactcctt
     1321 tgaatggttt tcgtcgactt tattctaaag gatttcaagg tgtctgtttg cgtttattag
     1381 aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca ctagatatta tagatccaga
     1441 aaagcagatt gtttatgcca aaattaagac attgcaaaaa gattattttc tttaaaatcg
     1501 acaagatgac cagaaagcaa gagttcgttg agctcttgct ttcttttagt tttagaaagg
     1561 aacatcctga ttttatcttc gctaaatcca gtcaaaagtt ttgcagattg gtcgtagata
     1621 atcggttttt tcaataaaga aggggatgta cgaatctcaa tatatagttc actcaaactg
     1681 agttcatcta ttctgtgaac aagattttta taactaatag aatgagtaga gagcatttcg
     1741 gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt catttttggt tagtggttga
     1801 cgaattaagt tctgttcgat aaacggtata tcatggacga taagccaacg ttttgcgcgt
     1861 cttgaagacg agcagccaga acctgtatac aatttaatca tcatacattc ctccaatagc
     1921 taagtattta tgaaataata acatgaagaa atagtcgaaa agtgccattt agaggtgtaa
     1981 aataagaaaa tgttgtcacg tataatggaa ctagctagat aagaaatact attaaatcaa
     2041 gaaaaaagaa agaaggcaca agtatgtcaa acacagccga cgcaatagta gtgatcgatt
     2101 tacaaaaagg ggtatgtttt gattcttcgc cgatacacga attagaaaca gtgacagcat
     2161 tagtgaataa aagaatcaaa acatacgaaa cgattgggaa accagttatt ttcgttcaac
     2221 acgaagatgc tcaattagtg ccacaaacaa aaccatggga gctattgtcg gaattatcta
     2281 ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa ttcattttat aagactaatt
     2341 tgagtgtttt attaactgaa ttggactgtc attcgatcga attatgtggg gcacaagcag
     2401 agtattgtat agatacgact gtgaagtttg ctcatggatt aggataccag cttatgatgt
     2461 gtagaggagc aacgacaact tatgataatc cttttatgaa agcaaaggaa acaatcgctt
     2521 tttacgagaa aatttgggat aaacgatttc tgacattttt ggagcaataa gttgtttctt
     2581 cgtttaaaaa aagcagaatg gatactggtt tatccattct gctttattac gtaaaatgag
     2641 aaaataatcc tgttctattt ttatgggata aaccatttat tttttgtata aaaaatgttt
     2701 gcgtttacat ataattctcc tagaaacata aaaaggagtg aatagcttga gaacaaaaca
     2761 agtattaaag ggagcgttgg tcatcttatt tgctgtaagt acaagcgtcc cgcaatggac
     2821 agtatatgga gagagcattt cttccgaatc ggaatctcgg gaggtagaaa ccacagaaac
     2881 tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa acagaaacaa atactgaaac
     2941 aacggttgag acatctgtta ctagcacaga tcaagaaacg accgctacca cagataacca
     3001 acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac gaatcctctt ctgctaaagt
     3061 aagtgacaaa gagaatggga cagaaaatga tgatatcggt aaaggtgaaa atttcaatgg
     3121 taaagaatat ttgaattact tcggtaaaca gatgacagaa atacttaatg gaccaacaga
     3181 agacatcaag tcaaaattaa tagagttatt agtcactgag cttgctgtta atgattatgg
     3241 tgtagataaa gagattatca aacatctgat tgctacttat ctattgtacg atatcgcttt
     3301 tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca gatgatggga caacgcctac
     3361 agaaaacaca ggcttctatc caaatacaaa acaacttcat caaactgtac taaacaaaca
     3421 agatggcaaa atgatggatg tatatgcata ttatgtcgat caaggttcag ataaaacagt
     3481 catgatccat ggcggatttc gagggaattg gaataacggg atcgttacag aagaatacaa
     3541 tgatttttat aaggccgggt ataatttatt atttgttgat tccagagcga ctggtaatag
     3601 cggaggcgat tatgtgacct atggacaata tgagtcagat gacgtccttt actggataaa
     3661 tcaagaagtt cgagagcggc catctcaaaa gattttactt tatggtggtt cgatgggagc
     3721 agctacgatg atgtctgtat tagcgaaaga tattcctgtc aatgtcaaag gaattattga
     3781 aaattgcgga tttgcatcaa ttgatgaaca actacgtttt acgtacagcc agacagtagc
     3841 ccccgcacta ccggatgcca taaaaaatca gttagacatc attggggatc aagagcatga
     3901 agatttattt atgggccttt tgaaacaata ctattttgat caggagatgc atttggatac
     3961 aaaagctgct ctacctacga ttggaatgtc agattcattg cctaaattga ttatccatgg
     4021 aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa ttatatgagt tatcaggagg
     4081 gtataaagat cttttattag tagaaggagc agggcatgga aaggcacagg aagttgacca
     4141 tgctgcctac acgaaacatg taacggactt tttgaaagta gtgttccatg atcagataaa
     4201 cgtaaagtat gtggatgaaa acaataaaag tctactcaaa gaccaggacg aaatccagct
     4261 ttacggagct tatggagaaa attatatgac agagcaaaag acattcgaag gatacgaatt
     4321 agcaaatgtt gaaggaccga ctgaagggat ttttaatgag acgataccaa ctattatttt
     4381 ctattacaaa aaaatcccag tagctccacc aaaaaagcaa gatccgacag agaatgcaga
     4441 caataaggga aagaatgaac ctcgagagga tcaatcaagt gatgatacgc aaccgattca
     4501 gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa agaatgttgc cgaaaactgg
     4561 agaaaagaaa cattcgcttc tcatttattc aggtggattt ttatctgtta gcacgattat
     4621 cacatggatt tggcgaagaa agagagggta aatgaatgaa atggttcaag cgaatagtga
     4681 ttgcagtacc agttatcatc gtagttattt tagtcactgt atttttgatt aatcaaatca
     4741 caccaaagcc gattagcttg cttgttagaa aacaatttga cacaagtgaa aaagaacaag
     4801 tatatgcccg accagatgat ttccagcaaa aaatcgaaca aatcgatata aaaaaagaaa
     4861 tatcctatcc ttcaaagtac aaaaataata atatggatat ctacacaccg aaagtggaaa
     4921 agaaaaaatt accgattctt ttctggatgc atggaggcgc atatgtagga ggggataaaa
     4981 atgattgtcg tgattatttg gaatatctat gttcagacac gcagcaaatc atcgtaaata
     5041 tagattatga acgatcaccg gaagcaaaac atcctacacc agttatccaa ttgaacgaag
     5101 ccattcaagc agctaaaaaa gagataaaag atcaagcaga ttggtcgaat atttcgattg
     5161 gtggaaattc ggcaggtgca caaattagtg gagaatattt gttagcactt caaaatgaag
     5221 aaattaaaaa agctgatcat cttgatccga ctttggagaa caagcagatt acgaagttta
     5281 tttctttgtc tggtctgttg gatccttctg cacttactca agtaagtgat aaaatctcta
     5341 gctttcttta tgaaaaatgt ggatgggctt actttgataa taaggacttt gaacaatcag
     5401 agaaggtcaa aagtttagcc ttggtcagac atgcggatcg ttggacgcaa aacacattct
     5461 taacggatgg aaatacaaat acgtttacga aacaaatgga agaagtaact tctgtatttg
     5521 agaaagcggg ggtgaaagtc acaaaagtag attatgcgga gaaaaatgct gttttgaacc
     5581 atgaatatca gtttgatttc agcaataaac aagcgcaaga aacatatcaa aagttagtaa
     5641 tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt gtaatctgtc acttagctcc
     5701 aagtcacaag gagcaatttc aataaacaag cttttcatgg tttatgaata ttgtaacttg
     5761 tgactgagga gttagagatg gcaaggggca ccttttattc gaaatttggg aatatcacac
     5821 gattattgtc acgatctctt ttacgtaaat tttataagtc agatttgttg ttttgatcgt
     5881 ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa gggacaaaaa gccgagtggt
     5941 aagcaaaaga atgctggttc cactaatatt ccgttgttat tgatataagg ctgggaacaa
     6001 agatgaatag caaataaaat caatgcacta gcgaaaaaaa gcaaagaaat atggatagct
     6061 ttcataagca gcacctcctt ttttataatt gtatgataaa tagattaaga atctataaat
     6121 gtgacaagtt tgttaggatg tgaataaaat gatcatctag cgaacttgaa acaaagatgg
     6181 ctaatgataa gcaattaatg attcagcaat cagataggta caaagtttac catcatacgt
     6241 ctagaccaat aatagaaaat atgggaatat aatagaaaag atttaggata cccccaaaag
     6301 aatttcccca aaaattcgat gtatcctaaa c
//
LOCUS       DAZKAS010000139         6287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000139 DAZKAS010000000
VERSION     DAZKAS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.141"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG8H7_002372"
     CDS             190..690
                     /locus_tag="ACG8H7_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP8984439.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACG8H7_002373"
     CDS             787..2634
                     /locus_tag="ACG8H7_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFP8984440.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACG8H7_002374"
     CDS             2637..3533
                     /locus_tag="ACG8H7_002374"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8984441.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACG8H7_002375"
     CDS             3583..3972
                     /locus_tag="ACG8H7_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP8984442.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACG8H7_002376"
     CDS             3959..>6287
                     /locus_tag="ACG8H7_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8984443.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAZKAS010000140         6190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000140 DAZKAS010000000
VERSION     DAZKAS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.142"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..244)
                     /gene="rpmB"
                     /locus_tag="ACG8H7_002377"
     CDS             complement(56..244)
                     /gene="rpmB"
                     /locus_tag="ACG8H7_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP8984444.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            514..876
                     /locus_tag="ACG8H7_002378"
     CDS             514..876
                     /locus_tag="ACG8H7_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP8984445.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            928..2610
                     /locus_tag="ACG8H7_002379"
     CDS             928..2610
                     /locus_tag="ACG8H7_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFP8984446.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2727..4763
                     /gene="recG"
                     /locus_tag="ACG8H7_002380"
     CDS             2727..4763
                     /gene="recG"
                     /locus_tag="ACG8H7_002380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP8984447.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4812..5813
                     /gene="plsX"
                     /locus_tag="ACG8H7_002381"
     CDS             4812..5813
                     /gene="plsX"
                     /locus_tag="ACG8H7_002381"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP8984448.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5935..6177
                     /gene="acpP"
                     /locus_tag="ACG8H7_002382"
     CDS             5935..6177
                     /gene="acpP"
                     /locus_tag="ACG8H7_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP8984449.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2060 a         1076 c         1416 g         1638 t
ORIGIN      
        1 aatttttatt aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa
       61 cgcgttcaac ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc
      121 cgtcgaccat aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca
      181 tagcgtgtga acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg
      241 ccatggtaat ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag
      301 actaacatat catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat
      361 tgagaagaat aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt
      421 actttgggta actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa
      481 tgggagtttt ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct
      541 ggaaccattg aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat
      601 gtgttcggtg ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt
      661 ttgcgtaaag aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct
      721 gtagatgttt atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc
      781 caagaaaaag tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat
      841 gtctttgtac aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag
      901 cagtcgatct gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc
      961 caggaaatgg tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca
     1021 ttaaatgtat tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact
     1081 agtggagcta aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg
     1141 ctttcaaaag gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg
     1201 tttcgtggat tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct
     1261 gcagccttca ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga
     1321 acgattttga cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact
     1381 gatgatgtcg ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa
     1441 actccagact tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga
     1501 ctgctgtttg tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa
     1561 acatatgaac cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt
     1621 gtccaaggtc aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta
     1681 aaaatcggag aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat
     1741 ttagatggta taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac
     1801 gtgcatacag aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc
     1861 aaaatcaaag tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa
     1921 gtgaacgagt ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct
     1981 gctggtaaag gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt
     2041 ggacaaacaa tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca
     2101 gataaagtga ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca
     2161 gaagtagcag atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca
     2221 gctatgcttt cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa
     2281 atgttgtcta gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac
     2341 ggtgtagaga tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct
     2401 aacccagata tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca
     2461 gagatcgtaa cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct
     2521 gagaaactaa cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct
     2581 gtctatcctt atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag
     2641 tattcattca taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt
     2701 tttgttaaag gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg
     2761 caggagtcgg agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag
     2821 acttactgac ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga
     2881 tccaggatca agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc
     2941 gatttggtta taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca
     3001 acgtttcttt ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg
     3061 ccgtatacgg taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt
     3121 ctcaaacaat ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga
     3181 cattagttga tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc
     3241 tgccaaacaa ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta
     3301 tgcattttcc aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag
     3361 agttcttttt atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga
     3421 atggtttgga gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt
     3481 ttgaactgac aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc
     3541 cgcagcatat gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg
     3601 cgattgcgtt atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag
     3661 aaatcttggc acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa
     3721 ccatcgcatt gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac
     3781 tagagcaagg ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg
     3841 aatttcagaa tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc
     3901 ggaaagtact tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga
     3961 ttcctagaac attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac
     4021 ttccagctgg acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg
     4081 tactggattg gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc
     4141 tgattgaaga atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc
     4201 gtgaactatt tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg
     4261 aaaaagaagt aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta
     4321 ctgtaattga agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg
     4381 atcgatttgg attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg
     4441 cttcctactg tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa
     4501 tcatgacaga gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc
     4561 ctggtgaagt atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg
     4621 tgacggatgg aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa
     4681 aggattggca gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc
     4741 aggataatcg ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag
     4801 gagtggaaac aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa
     4861 ttgtagaagg cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg
     4921 gaaaagaagc agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata
     4981 cagacgaaaa aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg
     5041 catcaatggt ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag
     5101 cagggaatac aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata
     5161 tcgaacgccc tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg
     5221 atatgctgga tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta
     5281 ttttaggttc tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct
     5341 taaacaacgg aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc
     5401 ttcagaatga aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg
     5461 gtgtggctga tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg
     5521 aaggaacagc aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca
     5581 aaggaaaaat gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg
     5641 attattccaa acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc
     5701 atggagcaac aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg
     5761 agacagatgt agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg
     5821 atatctgaag cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa
     5881 aattagccat ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact
     5941 cgcgaagaag tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc
     6001 gataaagtga cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt
     6061 atggaattcg ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct
     6121 gaacaaattg aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt
     6181 caaataaaag
//
LOCUS       DAZKAS010000141         6121 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000141 DAZKAS010000000
VERSION     DAZKAS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.143"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2149
                     /locus_tag="ACG8H7_002383"
     CDS             <1..2149
                     /locus_tag="ACG8H7_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFP8984450.1"
                     /translation="KSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLE
                     GTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKG
                     NVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQK
                     GLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFE
                     YSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALAS
                     ATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAA
                     GAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHL
                     IQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVAN
                     NRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHS
                     KTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFF
                     FSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQR
                     QRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKAC
                     DQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2118..3533
                     /locus_tag="ACG8H7_002384"
     CDS             2118..3533
                     /locus_tag="ACG8H7_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP8984451.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3566..4600
                     /gene="sppA"
                     /locus_tag="ACG8H7_002385"
     CDS             3566..4600
                     /gene="sppA"
                     /locus_tag="ACG8H7_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP8984452.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4602..5123
                     /locus_tag="ACG8H7_002386"
     CDS             4602..5123
                     /locus_tag="ACG8H7_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFP8984453.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5120..5506
                     /locus_tag="ACG8H7_002387"
     CDS             5120..5506
                     /locus_tag="ACG8H7_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFP8984454.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2179 a          847 c         1143 g         1952 t
ORIGIN      
        1 aaaatcattt ccttttgtgc agcaacaaga tgaaaaagat tgtggtgttg cttgcctttc
       61 gatgatttta aaatatttta aaactgatat cccgattcat aaattacggg acctatctgg
      121 gactgattta gagggtactt cagcatttgg attgaaaaag acatttgaaa aattgtcttt
      181 tgattgtatg gctattcaag cagatagtga gatctggaaa gaaagagatc ttatcttacc
      241 attaattgca catatattgg ttgataacag ctatatgcac tacgttgtaa tttataaaac
      301 gaagggaaac gtactatgga ttgcagatcc cgctaatgga aaaaatagaa aaacgattga
      361 agcgtttcaa aaagaatgga caggtattct tttattacct atgcctagag aaacttatat
      421 tcctattaaa gaaaaagtag ctggattaag ttcttttttg cctataattt ggaaacaaaa
      481 aggattagta ttccacatcg ttttagcatc gattttcatt acgttttttg gcataggaag
      541 ctcatattat tttcaaggta ttcttgattt tttcatccct aatcaagcac gttcaacttt
      601 aaatataatt tccattggct tgatcgctgt ctatttattt cgggtattgt ttgaatatag
      661 tcgtagttat cttcttatta ttttaggaca gcgaatgagt atggcaatta tgcttcagta
      721 tttcaaacat gtgttaactt taccaatgaa tttttttgct acgagaaaat caggggaaat
      781 tatttctcgt tttcttgatg ccagtaagat tattgatgct ttagcaagtg ctacgttatc
      841 aatattttta gatattggta tggtgttgct tgttggggtt acgttagcta ttcagaatag
      901 tacgttgttt ttaatcactc tatgctcttt gcctttttat gtagttgcaa tattcgcttt
      961 cgtgaaaagc tatgaaaaat cgaacaggga agaaatggca gccggtgcag ctgtaaattc
     1021 tagtatcata gaaagtctta aaggaataga aacaattaaa gcatatagcg gtgaagagaa
     1081 agtatataac cgtatagacc atgaatttgt taaattaatg aaaaaatcat ttcgtacggt
     1141 tacgcttgat aatattcaac aaggaattaa acatttgatt caattaatta gcagtgcatt
     1201 aattttatgg ctaggttctt attttgttat ggataataga attagtctag gtcaacttat
     1261 cacatacaat gctttactag tatttttgac agatccttta caaaacatta ttaatttaca
     1321 agtgaaaatg caaacggctc aagttgcaaa taatcgttta aatgaaattt tcgcgattga
     1381 ttcagaaaaa tcgaaccagt cttttgaata tatgctttct gaacaattat ttcaacaaga
     1441 tatcactgta aaaaacgttt cattctcgta tagcatgaag gccccaacgt tgaacgaaat
     1501 ttcatgtaca attccttcgc acagtaaaac ggctatagtt ggagttagtg gttctggaaa
     1561 atcaactctt gcaaagttat ttgttcgttt ttatgaagca tcagaaggat cgatccatta
     1621 tggaaacatt aatcacttag acattccttt tcagatatta cgcgatcgag tgacatattt
     1681 gcctcaagag tcatttttct ttagtggaac aatcattgag aatcttatat tcggtttaga
     1741 tagcccacca agtttcgaac aaataataga agtttgtgag atagtccaac taaatgaatt
     1801 tattaatcaa cagccattac gttacgagac attgttggaa gaaggtggaa ataatctttc
     1861 aggagggcaa cgacaacgat tagccttagc acgggcttta ttaaaaaatc cggacatatt
     1921 aattttagat gaagcaacta gtggattaga taccttgtta gaacatgcaa ttatgaatta
     1981 tctcctcaat ttaaaagaaa aaacgattat ttttattgcg catcatttac caatagcaaa
     2041 agcatgtgat caaattttag ttctccatga agggcatttg gtagaaaaag gaacgcacga
     2101 tgaacttcgt tttgccgatg gtatctatca aaaattatgg gaaatctagg agagggaaac
     2161 aatttatgca gaataataat tggttcgaac attctagtat ctatactaaa caacatcata
     2221 gcttctaccg ttgggtgtta taccctgtaa ttcttttttt atgtattatt ggattgtttt
     2281 taacctttgc taaaaaagag gttgttattc ggacaacggc tcaattgaac cccgaaaaaa
     2341 tagagaagtt acaagtgcca ttagaagcta aaataatgga aaacttccta aaagaaaatc
     2401 aatttgttca taaggacgat gtgttggtta gattggattg ttctttaata gagaatgaaa
     2461 aggctcagat agaacaagaa aaccaacgta ttactcaaca aataaagatg gctcagctat
     2521 ttattgaaag tataagtaaa ggaaaaaatt tgttttcaac ggatgacagt tttggctaca
     2581 gtaatcaatt aaagagcatg ttgtcagaaa aagaatcact ccgctacgct ttgaagcaaa
     2641 gtgaattaaa tgatcaaaag caattagaag tatacgaaaa gacaaaaaga caactagaaa
     2701 aacaaattga gagttcagat agtaaattac aagaatggca acaagtacag gtagcttgga
     2761 gtaataatca atcattaaaa gatttttcaa aagaaatgat ggcaaactat gagaattggc
     2821 aagaacaact aaataatgtt tctgatgatc aaaaaaatca agtgaaactg acaatttcag
     2881 caagcataaa tgaacaaatt gagcaactaa aaaaagaagt agaacagtat cagtcagaaa
     2941 aagctaaatt agttaaacca actacttctg agaatgacag aattagtcaa acggaaaaag
     3001 gaaagcaaga gctagaacaa gcaataacaa ctgtaaaaca aacagttaca gagctaaatg
     3061 aaaaacaaga gaagaaccag tcagttatca aaagtttgga cgatcaactt tcaaaaggaa
     3121 tactaaaagc tcctgtatct ggaacggttc atttaaatga agaaacaaag ggacaagcag
     3181 aactagccaa aggaacagtg cttgcagaaa tttatccgat ccacgaaaaa tcaaaaatga
     3241 aatttacagc acttttacct gcgaatgaaa gtacctatat taaagaaggg atgaaggttc
     3301 attttaaatt ggatcaaaaa ggtggggctc cgattacgat cgacggaact ttagatgaaa
     3361 tttcagaaaa ttctactgcc actgagaaag gtgtttttta tgcagtgaaa ggaacgttaa
     3421 aaacaactca atcgtttccg tttcgttatg gattaactgg agagttgtct ttaattgtag
     3481 gagaaaaaac atattggcaa caaattgtcg atgtgtttct aaataaaaaa taattatttt
     3541 tactaagaat ataggaggag tttatatgaa taaaagaaga tggattgctg tgggagtagc
     3601 gtgcggatta ttgttactat ccattatcgt cgcgctaatt ccaggaaaag ataaagagga
     3661 agcatcaaat acaaccctaa caggaataaa taagattttt tacggatcta acgaaatcac
     3721 agaagaaacg ttagaagaag gcgcatcaaa taaaaagatt gtcaaactat ccgtcaatgg
     3781 agttattgca gatacaggtg aaagcaatct ttttagtagg gaacaatata accatcagaa
     3841 ttttttaaca cagctaaaga aaattcaaga agacaaagcg gttaagggag tgttattaga
     3901 agtaaattca cctgggggag gaatatatga aagtgctgag attgctaaag aaatggcaaa
     3961 aattaaaaag ctagatattc ctatctatac tgcatttaaa aatacagcgg caagtggagg
     4021 gtactacatt tctgctggtt cagataaaat atttgctacg gaagaaacaa caactggatc
     4081 aattggtgtc attatttctg gtttaaatta ttctggctta ttagaaaaac ttggcgtaac
     4141 tgatgcgacg tataaaagtg gtgcattaaa agatatgatg tcacctcaac acaaaccttc
     4201 agaggaagag aataaagtta tacaggaatt tgtgatgagt gcttatgatc gttttgtcaa
     4261 tgttgtagca aaaggaagaa atatggatac taatgcagtg aaagaacttg cggatggaag
     4321 aatttatgat ggtaaccaag cagtagaaaa tgggttggtg gatcaaattg gttactctga
     4381 agatgcatta gattccctaa aaaaagagaa gaaattaact gatgcgacgg ttatagaata
     4441 taaaaatgac acaactggct ttgcttcttc ttggttaggt aataagattg ctgaatggca
     4501 agggctaaag gcaactacat cagatcgtgt aatttcagtt tttgaaaaac ttggattttc
     4561 tgatactcca aaaccaatgt attactatgg agggctataa aatgaataag aagagtggca
     4621 ttgaaacttt gcctaattat gtatttgcgg gattttggtt acgatttttt gcttttctta
     4681 ttgatttaat ttgtattagc atcatcaccc atgctactat aggtctcttt tacaaaatca
     4741 tgggactgga atctgacaaa tccattatta ctttttatgg tatagcgtca ttagctattt
     4801 atttaaccta ttttgtactt ttgactaagc tgaattcagg acaaacaatt gggaaaatga
     4861 tttttgggat aagagtagtg agctttgagg aaaaagagct aagttggcaa actatcttta
     4921 ttcgtgagtg tatatgtagg tttatattaa aaaattttgt atttcaaatt gggtatttgg
     4981 tggctgcttt cacgcccaag aaacaacata ttggggatta tttttctaac acgtcagtgg
     5041 ttgttattcg ttcattagaa gctattaact atggtcaaaa aatttctaat tctaaaacac
     5101 ttaatctgga gagaattaaa tgaataagag aaaaacagag tttattttga ttttaattag
     5161 tggaataaca gggattttat gtagtctttt agcattgatg ggtggtatgt ttctatcttc
     5221 tattgattca actactttaa atactacaga actaaccagt aaggatatag atatgttagt
     5281 caatctaagc gggaatgttg ttttatttgg aggaattacc ctagtttttt cgataatgat
     5341 tttcataata ggttttttag taagaaaaaa tcaaaaaaca gtggtctatg gtattagttt
     5401 actcatttta tctattattc cattttttct tttaacatta ttgtgggtaa ttcctggagt
     5461 tttaggtatt attgcgggat tgatgcttct aataagaaaa tcttaattag gcaatgtgta
     5521 gacaacgtga aacgattaat caattaaaag ctaaatacgt tttgagaaat gataacttgt
     5581 agtcgatgga agacatgtag ataaaaaaac tacgtgtctt ttatttattc taattttttc
     5641 gaaaaaaaga cactctctga ttgttgtatt aatggaaaag gggaaagcag caagtaaatt
     5701 tagtttttct attaaatatt cttcactttt taacctttta tagtacttag atttaatcct
     5761 atatgtttag agagttcatt ggcagcatga ttttgcaaag cttctcgaat tattgttata
     5821 ttagaaactt tactgttcat aattatgctt cttttttaac tcaaagtcca aagggctaca
     5881 gtggactttg agttaaaaat aaaaagccct agttattttg aaaaataaaa aaagaacagc
     5941 gtaaatgttt tcgaaatgct ttcgcaccaa catttacctg ttccgtgggt ttggcaccca
     6001 atgggccgtg aggggctcga acccgcgacc cgctgattaa gagtcattaa tgtcaatttt
     6061 taaataaaat taatatgtct ctttaagtat taaactatca ctctttatcc tatgataaat
     6121 c
//
LOCUS       DAZKAS010000142         5872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000142 DAZKAS010000000
VERSION     DAZKAS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.144"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..1083
                     /locus_tag="ACG8H7_002388"
     CDS             76..1083
                     /locus_tag="ACG8H7_002388"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFP8984455.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1095..2099
                     /gene="neuB"
                     /locus_tag="ACG8H7_002389"
     CDS             1095..2099
                     /gene="neuB"
                     /locus_tag="ACG8H7_002389"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFP8984456.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2096..3244
                     /gene="neuC"
                     /locus_tag="ACG8H7_002390"
     CDS             2096..3244
                     /gene="neuC"
                     /locus_tag="ACG8H7_002390"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFP8984457.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3217..3894
                     /locus_tag="ACG8H7_002391"
     CDS             3217..3894
                     /locus_tag="ACG8H7_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP8984458.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3845..5026
                     /locus_tag="ACG8H7_002392"
     CDS             3845..5026
                     /locus_tag="ACG8H7_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP8984459.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5039..>5872
                     /locus_tag="ACG8H7_002393"
     CDS             5039..>5872
                     /locus_tag="ACG8H7_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFP8984460.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NN"
BASE COUNT         2179 a          747 c         1138 g         1808 t
ORIGIN      
        1 attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga ctataattca
       61 taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg aactggttcc
      121 attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat cagaatttat
      181 agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca cactaatttg
      241 agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg tcgtgatgtg
      301 gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata taatcctatg
      361 gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc gatcaaaaat
      421 aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac taacaccatg
      481 ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta taaaggaaaa
      541 acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag tggatctgtt
      601 attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac agatgttgat
      661 atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc tgctgctatg
      721 aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat tttaacttta
      781 gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag aggtgaaata
      841 aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac tacagacgaa
      901 attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta tcatgctact
      961 gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc agatgcaaca
     1021 gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt cgtgacttgg
     1081 taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt gtaaaccata
     1141 atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt ggagctaacg
     1201 cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct ccaatggcaa
     1261 aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta aaaaaattag
     1321 aattatctca atctgatttt attagaataa aagattattg tatacaaaat aaaattgatt
     1381 ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt ggtgtacaag
     1441 gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa atagatatgt
     1501 atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc agtgaggctg
     1561 tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat tatccggcta
     1621 gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc gggatacccg
     1681 ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt gcgatgggtg
     1741 ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca gatcataaag
     1801 cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac actgaaatat
     1861 ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa gaagtatcaa
     1921 gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca gagaaatctc
     1981 ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa tatattggca
     2041 aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat gttgaatgaa
     2101 aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac ctgttgctga
     2161 gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc atttatccca
     2221 taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta gtggagaagt
     2281 tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg cattagagat
     2341 aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat tactagggga
     2401 tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca aaacagcaca
     2461 tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg ccataacgaa
     2521 aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa taaaaatggg
     2581 ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta ttttaaataa
     2641 tagattacct agttttagag atataaaaga aaaatatcat ctaaaatttg aaaagaaaaa
     2701 atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag aaaaacaaat
     2761 tttagctttg atcaattcaa tggaacaaca atctcaacca attatttgca ttttacctaa
     2821 tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa aaacggatat
     2881 tatttatatt acgaatttag agccggagga atacctgact atattaaaag agtgcaaatt
     2941 tatgatcgga aattcttctt ctgggataat tgaagctgca tcattcaaaa taccagtaat
     3001 aaatttagga accagacaaa atggacgaga acaatctaaa aatattataa atgcaaatct
     3061 aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa gatcctcagt ttataaaaag
     3121 cttgaaaaat gttaagaaca tctacggtga cggcaacagt tctagaagaa taacaaaaac
     3181 attgaatcaa atattgaatg atggggaaga tattaagtgg attcaaaaac aaatagctta
     3241 ttaaaatata ctagtgatcc atacattatt attggggctg gcggacacgc aaaagtcatt
     3301 attgaaacct taagattaga aaataagtta atatatggat tttttgatga taagcaaaat
     3361 aaaatgcaag atctgaaata tcttgggaaa ataaacgaat tgaatagctg ggcggatagc
     3421 aaaaaaaaaa tctataaatt tattgtagct attggtgact gtgtagtaag aaaaaagatt
     3481 ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag ctgttcatcc aagtgctata
     3541 gtggcaaaag aaatttcaat tggttatgga actgttattt ttgcgaatgc tgtaattaac
     3601 ccagatgctg taattggaga gcatgctata ataaacacag gtagcattgt tgagcatgat
     3661 tgtagaatta ataactatgt tcatttatct ccaggtgtat gcttagcggg tggagtacat
     3721 gtaggtgtag gaactcaaat aggaattggt tctcaatgta tccagatgaa gaaaattgga
     3781 tcttggtgta tgataggcgc agggagcact attgttaagg atattccctc acattcctta
     3841 gcttatggca atccagctaa aattaagaaa gaaggaatta attttgaatt ctagaatttt
     3901 attatcaagt cctcatatgg gaggaacaga agaaaaatat gttgctaagg cttttaaaga
     3961 taattggata gcgccattag gtgaaaatgt ggataaattt gaagaaagta ttacaaaata
     4021 tactggttct aagggcggag cttctgcgtt aagttcaggt acagctgcca tacatcttgc
     4081 cttaagactg cttaatgttg gaaaaaacga tattgtcttt gctccttcat ttacttttat
     4141 agctacagta aatcctattt tatatcaagg ggctgtacca gtgttcattg actcagaacc
     4201 taatacatgg aatatgagcc ctgttgcact agagagcgca ttagaagaag ctgaaagaaa
     4261 caaccaatta cctaaggcaa tcatcgtagt gaatttatat ggtcaaatgg ctgattatga
     4321 acgaatttta gctattgcaa accaatttaa tgttccagtg atagaagatg cagcggaatc
     4381 gttaggttct acttataagg gaatttcgtc aggcttattt gggacaattg ggatctattc
     4441 atttaacggt aataagatca tcacaacctc aggtggagga gctctagtgt ctagtgataa
     4501 aaaatatgtt gagcattcaa gatttttagc aacacaagca agagatcaag caccgtatta
     4561 tcaacatagc gaaataggtt ataattaccg tctaagcaat atttctgccg gtatcggacg
     4621 tggacaaatg gaagtattga atgatagagt agctaaacga agagaaattt tcaatcgata
     4681 taaggaagct ttttctggaa ttgatagaat tgaaatgatg ccagaagtgg atggcatggt
     4741 gccaaatcgt tggttgtcta ctttaacgat caatgattat aattctattg atcaagtagt
     4801 taacgtaata aataaactaa atgaaaaaaa cattgaaaca agagctttgt ggaaaccact
     4861 acatttgcaa ccagtatttc aaggggtaaa aatgtattac catgataata atcttagtgt
     4921 gtcagaaaag ttatttaata gcggcttatg cttaccttct ggaagtaata tgacagatag
     4981 tgaacaagaa tttgttattg atgagtttaa aaaaatattc aagtaggtgt gatattttat
     5041 gaacatagga attattggtt taggttccat ggggaaaaga agacttagac tcattcaaga
     5101 acactttaat gatattttac tatgtggtgt tgatactaat gaggcgagac gaaaggaagt
     5161 tgaaaaggaa tttggaatac caacttataa tagtctaaca gaggcaaaaa atttgtttga
     5221 aataaattca gtttttgttt gtacatcccc tataacccat gaatttatta taaaagaggc
     5281 tttaaattta gattgtaata tctttacgga aatcaattta ttaaatgaat attatgatga
     5341 aataatcgaa gtagctaaaa agaaagatag attactttat ctatcttcta catttttgaa
     5401 gagaaaagaa attcaatata ttaagtctaa agtgacgcat aataaaaaaa tttcttatca
     5461 ataccatgta ggacagtatt tacccgactg gcatccttgg gaagattatc gcaatttttt
     5521 tgtttcaaac aaaaaaacta atgggtgtag agaaatattc gctattgaat taccttggat
     5581 tatagataca tttgggaaag ttaaatcgtt tactgtaatg acccagaaat tatccaatct
     5641 tgatattgat tattcggaca gtttcactct tattattgag catgaaactg gtatagttgg
     5701 aactttaagt gtagatgttg tttcaagagt agctaaaaga aatttaacta taattggtga
     5761 aaagatttta atagaatggg aaggtaaccc ggattctcta ttttcatgga atgaagaaac
     5821 ttctagtatg acagatattc aattgtacga tgaggttatg catgataata at
//
LOCUS       DAZKAS010000143         5841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000143 DAZKAS010000000
VERSION     DAZKAS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.145"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8H7_002394"
     CDS             <1..395
                     /locus_tag="ACG8H7_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP8984461.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACG8H7_002395"
     CDS             398..1228
                     /locus_tag="ACG8H7_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFP8984462.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACG8H7_002396"
     CDS             1244..2095
                     /locus_tag="ACG8H7_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8984463.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACG8H7_002397"
     CDS             2092..2451
                     /locus_tag="ACG8H7_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFP8984464.1"
                     /translation="MNKYRNKKTVHRGIKSDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACG8H7_002398"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACG8H7_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACG8H7_002399"
     CDS             2624..2929
                     /locus_tag="ACG8H7_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984465.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACG8H7_002400"
     CDS             2929..3240
                     /locus_tag="ACG8H7_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP8984466.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACG8H7_002401"
     CDS             3237..3410
                     /locus_tag="ACG8H7_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984467.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACG8H7_002402"
     CDS             3407..3823
                     /locus_tag="ACG8H7_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP8984468.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACG8H7_002403"
     CDS             3823..4491
                     /locus_tag="ACG8H7_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFP8984469.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACG8H7_002404"
     CDS             4488..4865
                     /locus_tag="ACG8H7_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984470.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACG8H7_002405"
     CDS             4862..5161
                     /locus_tag="ACG8H7_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984471.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACG8H7_002406"
     CDS             5151..5309
                     /locus_tag="ACG8H7_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984472.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACG8H7_002407"
     CDS             5403..5819
                     /locus_tag="ACG8H7_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP8984473.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2172 a          886 c         1354 g         1429 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtctg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg a
//
LOCUS       DAZKAS010000144         5817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000144 DAZKAS010000000
VERSION     DAZKAS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.146"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..270
                     /locus_tag="ACG8H7_002408"
     CDS             25..270
                     /locus_tag="ACG8H7_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984474.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            333..755
                     /locus_tag="ACG8H7_002409"
     CDS             333..755
                     /locus_tag="ACG8H7_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984475.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(849..1166)
                     /locus_tag="ACG8H7_002410"
     CDS             complement(849..1166)
                     /locus_tag="ACG8H7_002410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984476.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(1193..1399)
                     /locus_tag="ACG8H7_002411"
     CDS             complement(1193..1399)
                     /locus_tag="ACG8H7_002411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984477.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(1380..1562)
                     /locus_tag="ACG8H7_002412"
     CDS             complement(1380..1562)
                     /locus_tag="ACG8H7_002412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984478.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(1576..1926)
                     /locus_tag="ACG8H7_002413"
     CDS             complement(1576..1926)
                     /locus_tag="ACG8H7_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984479.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(1952..2254)
                     /locus_tag="ACG8H7_002414"
     CDS             complement(1952..2254)
                     /locus_tag="ACG8H7_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984480.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(2257..2490)
                     /locus_tag="ACG8H7_002415"
     CDS             complement(2257..2490)
                     /locus_tag="ACG8H7_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984481.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(3394..3924)
                     /locus_tag="ACG8H7_002416"
     CDS             complement(3394..3924)
                     /locus_tag="ACG8H7_002416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984482.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(3921..5036)
                     /locus_tag="ACG8H7_002417"
     CDS             complement(3921..5036)
                     /locus_tag="ACG8H7_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP8984483.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(5042..5440)
                     /locus_tag="ACG8H7_002418"
     CDS             complement(5042..5440)
                     /locus_tag="ACG8H7_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP8984484.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         1704 a         1179 c          767 g         2167 t
ORIGIN      
        1 aaataactaa aaggaatgtc acacatgcaa cttaataatt taaatgaaat acatgattgg
       61 gctcaaaata atttacttaa tcgtaaacaa gctgccaaaa tcactggaca aaactatgat
      121 gctttttcta gctctttacg tagaaataat atcaaaccat ttctagaaat aggcgacgaa
      181 cctggtcctt ccatcattcg cttatatcta agagaagata ttgaaaaata cgcaaaacag
      241 ctcgaagaaa aaagacagaa acgtcaatga ggcgttttct gtcttggatt tacaagtaaa
      301 acaataataa acacttttac aacggaggtt ctatgaataa gaaatacatt gtttcagttg
      361 gttcaatcgt actgattgta ctcgctatta ttgctttttc tgtatccaac aaaacagaaa
      421 cacagccaca aaatacggtt caatcgacta ctacaagtca atctagcgat cattccgtct
      481 atccttcact aaatagttta aaagaaacgc atggacaaga tttaattgat cttggttctt
      541 ctcaaattac tactgtctta tttgaaaaga aaaatattgc gtttattact cctaaagaag
      601 gaactgacct tgaattttct atcaaagtaa caaataccaa agaaaattta ttctccattc
      661 ctgacattca atatacccct aacaagttaa agattgggga tacgtttggt ttggctaaag
      721 taaatgaaca atactttgct tactcactga aataagtttc taatagcaag aggaaaaacc
      781 ctcttgttat ttttatcaca taatcatttt ataaaaacgc ttcgaaaata tcaacatttt
      841 tgaagaaact atttttctaa tttctcaatt tcttccaagg gattttcgct aatgacccat
      901 acaggcacag attgctctcg aggctcccct tcttctaacc accaacctgc ataatgaact
      961 tcttcctcct cagcattacc atgataccaa ccaacaaagg attcatctac aacgaatgaa
     1021 atttcttctt ctggatcaaa taattcattt ttccatgctt caattgcttg ttctttggtg
     1081 tacttttgtt tgttaaatac accacaaatt tcgtcatcta cataaaaaat ttcaaaatca
     1141 aaattactcg ttccttttac tggcatctta acttttcctc ctttgactca cttcaataga
     1201 tataccactc atgttcattc cattgtttac tagttaattg gagttttatc ttagggcttt
     1261 gtgcactctt tccaaaaact tgagcgtcgt ttttttcttc taaccaaaaa aggtaatttc
     1321 ttacattcgg tactaaatgt ttttcctgca tttcttcatg aaaaacatat aattctgctt
     1381 catttttcaa ccaattcatt attcattccc tcattcactt cttcataccg ccattcagat
     1441 agtgagattt ctttttgttt ctccacttgt cctataaaaa tcgctgtatt caacaagctg
     1501 atcacactta ataataaagc aatgaaacta atggttacac ttactctttc acattttgtc
     1561 attttgtttc cttctttact cgtttgttat ttcttgtaat aggtctaata actccttcac
     1621 cgaaatttgc aaatggttcg caaaaatttc aataaaagca ctcgcacaca ttaccagacc
     1681 ttcaactgat atatcactgc gacaataaaa ttctggagaa acactgattt ctgattcttt
     1741 cttacaattg aacgaaaaaa ctatttttga atcaaattcc ggtaattctt ccacaactat
     1801 gttttgggat tctaccaaga aagggagtgc ctgttctaat gcttcttctg gtgtttgagc
     1861 agatagaata attgcttctt gttcttttat ttcattgttc tcaaatactt tataactttt
     1921 tttcattgtt agttcctctt tcattcctct ctcaattagc taagccgtta ctggtacgcc
     1981 attctctaaa tttagtatcg ctaatcctta atgctcgtcg aatatcaatc attcgataac
     2041 ctattgcacg aagttcttta tatcttttag cagttagcgt ctctaacgtc atttcaggca
     2101 atgggttcga atcaaaaata gaattcgaac cgattatttg ggccaattgt tgtacttgta
     2161 acagctcttc tgaacttaat tcagtgattg ctcgattgcc tatcattgca tacattttct
     2221 gtcgaatata cttcttttct tgtttagaca acatttctat ccactcctat tttcattatc
     2281 atttgttacc tccacttcag taaatccaca aataaatgaa ttcatattcc aagtaccatt
     2341 cccttcaact acaacttttc tcctgtcctc tttcggatat tcaagaatca gaccgtttac
     2401 caatatgttt ttcactacta ctaatttgtt tgtataccgt ggaattgctg ttccgatata
     2461 cttcacttta tttcctacgc taattgtcat ttcctcactc ctattctttt ttatccaaaa
     2521 ctttttcctg tcctattcct gccaacgaat atacttcact gcatgcttaa acaaaacaaa
     2581 atgttgccct gttttatttc cgtcttttac ttgttccaca tacagttcat atttggtcac
     2641 accttttaaa acgccagata ctttttttca tccattgtca caattagttt tttcatcaat
     2701 cgattgatac tgcactgctc atttttcttg ttcatatagt attcctttct ttttcaaaac
     2761 tagcctcttt tgtttactag ttttcatcaa tcatcaacga tttaacagat tttttctttc
     2821 gcagggtttc tagcgtcagc aaccccaaac tctaaaaaag tgcctttgct cccttgaaaa
     2881 acgagcggac gattgtactc gtcatcatat gtttttcata ctacctgatc cacgcaaata
     2941 accttttttt gttctgtgcc acacccatcc atacgtatcg cttgagattc accatacttt
     3001 tttaggtggt ttatccttcc taagtccctg ttagacacgc tgattaactt agaaagtcct
     3061 gtttgtatcc aattttcaaa gatctcccaa aagaaataag taggtttacg tgtatcgctt
     3121 ctgttcaaag tacctgtttg tacttgagta tttctactcc cctcgttcaa gggattcctt
     3181 ccaccagcta gcttttcctt tcatcatcaa atgttcctga aaagcattta cgtttcgtgt
     3241 ccgtgtgctt acttattctt ttttcgatag cgattatccg ctcgctactc gctattttat
     3301 actttgtttg ttagctcaaa taaaaactaa caaacaaaaa aggctagttt ctttccgttt
     3361 tattttttta actgtcgata aatagttgtt agtttaattc caagttctaa aaccaatacg
     3421 cccaacaaaa ccacaggaaa aacaattggg gcaaacaagt aaaatcggat agttttcatg
     3481 tcttccctct ttctttttgg tgtcgctgtg gtcgttcttg ccgttcaata cttcgataca
     3541 ttttttattc attatttgct cgttttgttt tcctctggac tgcttttctt gctttttgaa
     3601 tactttatac gcttttcgtg cctgaattaa taggtcttgt cggcgtttca ttcgtgagaa
     3661 aacaatattt gaaaccatca aatcctggtt cgaacaacca cgattcgcaa taattttccc
     3721 atcaaaaaaa atagttttca cttgagaaaa aaacagttgg aaatcttgtt ttgttgctaa
     3781 tttttttgtc aaaatcactg tgcttttccc tgttgaatct acaatgattc ttcgaacaat
     3841 ccgattcgat aacttccaac gttgccgatt aaacaatgtt acttcacaac aaatatgacg
     3901 taagtgatta ggggtcattt tcatgtgtcc aattcctcca accaattatg catcgaaatt
     3961 ttgggcaatt gtgacagccc ataaatctgc gtttccaggt cttcataact acaagaacgc
     4021 ttggcttgaa gtaatgaata ttctgcttga tctgatagtt ttgttgccat tggatctaga
     4081 ttcaattctc ttacacaaca ttcccaaaaa ttcaatagcg ctctaaaata aaatcctttt
     4141 cgattctgaa ttgcttttcc ttcatgacga gcaatttttt cattttgaat acaccgttga
     4201 tacgttttgc ttaaatcttc tgcaaatgtt tcccctaaaa ttcgatactc gcttccatca
     4261 gataaaccat gtgtttgatt atactttcgt tgccgctttt ctataattcg tttactgtcg
     4321 tatatcatgt ttagctgctc tttcgcttca cgccattcag ctgaactgcg aacaatccat
     4381 gtcaaaacat ctttcggaac aaacgtactt tcgctgtaat gagcaataaa ctctttttgc
     4441 caatcatcag ccatttcaac aggactagtc gtatcgattt ttgtatctag attttctgta
     4501 tctagattgt tagaagaatt attaaataat tgtgcagaat tttcacatgg atttttttcg
     4561 tcaaaatcat cgaaaatcgc taaattatca acagattcct cctcttcttt tccttgtgca
     4621 gaatctacac tggttgagtc aacaggacct aatgaataca catcattttc tgtcgtatct
     4681 aattctccta gatacaaccg agctttgttt ctttttcctg tttgttgatc aaatcccata
     4741 tcctctattt ctagtagctc atattctttc aattcttttt tgattgtatt aacagaacgt
     4801 ctgcaacaat tcaatgcatt ggctaattct tgttccgtaa attcaaaata aacaaaacct
     4861 tgactatcta cttgatttcg ttgaatggct aaatctaagc gtgaccgaaa aaccatatac
     4921 gcgatttttg ctttatcaga caatgagcga tatttttcac ttgtaataaa tacctttgga
     4981 aattgatagt ataattcatt gtatttacga gttgcggtaa atctttttgt tgccatcgtt
     5041 attacacctc atcttgtcca tatattaaga aagaattttg gtgttggtag acacgaacat
     5101 cttccattgt agcaatcaat tcttttagat aaattcgacc gaattcaatt gttagtaagt
     5161 cgtaatcttc taattcattt ttgatttctt caatttcttc ttcagacaca ttgagtaaaa
     5221 tggctaattc ttgattggta taagaaatat agacatgttt cttttcatca atccgttttt
     5281 caattaagga ttcttctaag tactcattca gtaagctata ggcgtatttt gcttgtgcag
     5341 tcatcgcata atattttgac gattttataa agaatagttt tggcatatga aaagttgctt
     5401 gtgtcagcac atctgttgcg cgaaatttgt tttttaccat attttttcct cctagaaaaa
     5461 cacgcaaaaa aagctctgta taaatcgaat tacacagagt ttcttcttgt tgatatttaa
     5521 tttctaaaat acgtttcggg taaaaactaa acacttacta atatttagat tgtagaatct
     5581 tcgccttcat ttattcattt taaagagaaa aagatcaagt gaaatgtgcc caaaagtttg
     5641 attctattaa tttttaaaca ccattcataa tatatttcct ataatgtaaa aagcgtcgat
     5701 acttattaaa gcatcgacgt tatttttata gtaaatttat caaaattttt gccaacattt
     5761 tttccgattt ttttccctat ttctatcttt ccttatcttt cttagttaat attattt
//
LOCUS       DAZKAS010000145         5809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000145 DAZKAS010000000
VERSION     DAZKAS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.147"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACG8H7_002419"
     CDS             450..1604
                     /locus_tag="ACG8H7_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFP8984485.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACG8H7_002420"
     CDS             1577..2020
                     /locus_tag="ACG8H7_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984486.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACG8H7_002421"
     CDS             3143..4075
                     /locus_tag="ACG8H7_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP8984487.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACG8H7_002422"
     CDS             4092..4607
                     /locus_tag="ACG8H7_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP8984488.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACG8H7_002423"
     CDS             4903..5184
                     /locus_tag="ACG8H7_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984489.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACG8H7_002424"
     CDS             5186..5602
                     /locus_tag="ACG8H7_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984490.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAZKAS010000146         5793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000146 DAZKAS010000000
VERSION     DAZKAS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.149"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..477)
                     /locus_tag="ACG8H7_002425"
     CDS             complement(19..477)
                     /locus_tag="ACG8H7_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP8984491.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(490..1821)
                     /locus_tag="ACG8H7_002426"
     CDS             complement(490..1821)
                     /locus_tag="ACG8H7_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFP8984492.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1832..3274)
                     /locus_tag="ACG8H7_002427"
     CDS             complement(1832..3274)
                     /locus_tag="ACG8H7_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8984493.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3261..4025)
                     /locus_tag="ACG8H7_002428"
     CDS             complement(3261..4025)
                     /locus_tag="ACG8H7_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFP8984494.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4129..5004)
                     /locus_tag="ACG8H7_002429"
     CDS             complement(4129..5004)
                     /locus_tag="ACG8H7_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8984495.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5259..5343)
                     /locus_tag="ACG8H7_002430"
     tRNA            complement(5259..5343)
                     /locus_tag="ACG8H7_002430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5307..5309),aa:Leu,seq:caa)
     gene            complement(5361..5432)
                     /locus_tag="ACG8H7_002431"
     tRNA            complement(5361..5432)
                     /locus_tag="ACG8H7_002431"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5398..5400),aa:Gln,seq:ttg)
     gene            complement(5442..5514)
                     /locus_tag="ACG8H7_002432"
     tRNA            complement(5442..5514)
                     /locus_tag="ACG8H7_002432"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5479..5481),aa:His,seq:gtg)
     gene            complement(5576..5660)
                     /locus_tag="ACG8H7_002433"
     tRNA            complement(5576..5660)
                     /locus_tag="ACG8H7_002433"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5624..5626),aa:Leu,seq:caa)
     gene            complement(5681..5751)
                     /locus_tag="ACG8H7_002434"
     tRNA            complement(5681..5751)
                     /locus_tag="ACG8H7_002434"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5717..5719),aa:Cys,seq:gca)
BASE COUNT         1755 a         1239 c          891 g         1908 t
ORIGIN      
        1 aacttttttc atcgtacact aaaattcaaa acaactatct ttaatcttta cttcttggtt
       61 ttgttttaat aaatttcctt caaaaatgcc tggagaaact acaatgatca ccgtatcttt
      121 ttgtgcttgc tccaacttct tcaaatctat aatagctaac agtgtccctt cttttacctc
      181 ctggttactt tcaacccgta tctgaaacgg acttccttcg agttcaactg tatctaaacc
      241 catatggatc agtatttttc tgccagattc actttcgatc gttatggcat gtcgggtagg
      301 aaatatatta tcgattattc ctttaactgg cgcatggata tatccatcat gattcgttat
      361 agcgtatcca actcctatca tttttgaaga aaagacctcg tcttttactg ctgttaatgg
      421 aataatttga ccactagcta cagcttttag aatgtttctt gttttaaaga aatgcacttt
      481 ttatcacctc tactttaatg ctggccaata ttttttgttt actttgatca tttcatctag
      541 tatttttttt gcgactctgg catttggtac ggtttgattc aatgtaaatg cttgtaaagc
      601 tttttgataa gaaccttcaa agtaagcatc tactagtaac ttctctactg caacttgtgc
      661 ctccatcaat cctttatgaa aatcactgat tggttttctt agtgtaattg gctccacacc
      721 agttgcacca acatatgccg gtatttcaac aactgcatct gaccgtagat tcggaatcgc
      781 cccttgatta ggaacgatta gcataaagcg acgacgatca tcattcaaca aggaaatagc
      841 tatatcgacg atatattgtc catgctcccc aaaattaaag ttcaatactt cttccacaat
      901 accgcggcgt attttatcag ccatttcttt ggtatttttc tctcgtccat ccatgaccat
      961 attagcacgt gtatactctt tgtcagtatg ctcaacgacc atatcaggat ataagtaata
     1021 ctctaagtaa ttattcggga tattacttgg aaaatttttc gtgatgactg acatcatttg
     1081 aaaggttttt tgccaagttt tatctcctat attaaaatcg gcgacttgca tttcttttgt
     1141 tattagtttt tcaataatct ctggcataat ttctcgttgc aattctttat cataaatcga
     1201 tgtataccag ccaaagtggt ttaaaccata atacgtaacg atccaattct tacgatcata
     1261 accgtaattc actgcaatcg tttcttcaat tgaaatagtc atatcacagg cattaatcat
     1321 ttttacattc ggatatttac gacgaacagc ttcagaaacg atcgactctg gatttgtata
     1381 attcaaaatc catgctttgg gggcatagtc ttgtatatga tcgacaattt ccaataaacc
     1441 tttgattgag cgcatcccat aagcaaatcc tccgagtcca cacgtttctt gtcctactaa
     1501 tccatgttta agtggaatct tttcgtcctt ttctcgcatc tccaatcctc caacacgaat
     1561 ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt ggatcatccg ttgcacgaat
     1621 gcgaatattt gaaaaacctt ttcttttcag cataaattta acaatcaagt acatatccat
     1681 atttctttct tcatcaatat catataaacg tatttcagat aatggcaacc gtttttcatt
     1741 gttcaaaatg gcttgaacga ttcctggtgt atacgtactc ccgcctccag ctatagtgat
     1801 aatctgtttt ttcatcatca tcttttcctt tctattcttc taataattta ttgaattctg
     1861 ccataacata agtcacttct aagccgatga taatttgata atttttttca gttggtttaa
     1921 tgatcccgct tatccctaat tgcttgattc gctgttcctc gaccatggtg ggatccacta
     1981 cctctacccg taggcgtgta ttacaactat agacatccag gatattggct tttcctccaa
     2041 acgcttgtag catttttgtt gccatatatt cataattttt acttgctaac cctaactctg
     2101 tttcttctaa ggttacttca ttgccaaact ctgccttagc ttcacgtcct aatgtctgaa
     2161 tgtttctctt attgatcaaa taataaaaag taacgtaata caaggcaaaa aagaaaattc
     2221 cgattggaag aatcaaaata gcattggtag cgattcgaaa attgatgaga taatcaacaa
     2281 tacatgctcc gaatgtaaaa ccaattcgta tattgaataa gtaacagaca atccctgcta
     2341 aaccggcaaa aagtgcatgg atcacgtaca acataggaga aagaaacatg aaactaaatt
     2401 caacaggctc agtgatattt gcaataaatg aagttcctgc accgcttccc atgattcctt
     2461 tgaccatctc tttattttct ttataagctg ctttatagat agctagacat atagctggta
     2521 taccaaacat cattactaca aaaaaaactg ataaaaacag tccagcctga ggatctccgt
     2581 tgataaagcg tgtcatctct cctctgaaaa cttccttgct aggagatgta tagcttccta
     2641 aatcgtaata aatatacgta ttcaacacat ggtgcagtcc aaaggggatc agcaaacgat
     2701 tcaaaaacat aaatatcccc acacctaatg ctcccatatc aacgagcagt tttgcaaaat
     2761 tatcaatccc aatttggatg agcggccaga taaagcccaa aaaaacagct gcgattgttt
     2821 gtagaatcat caccatagtc aatgggaatt tttcgcccgc aaaataagaa aaaactactg
     2881 gtaatttttg ttctttaaag cgattatacg tccatgccgc tactaaaccg gaaataattc
     2941 caccaaaaat ccccatattc acttctgggt ccataatttc taagcctttt ttcaaggtag
     3001 caatcgctaa aaaaccagta agtgcaggta ttcctttgtc cttatttttg acaaaaccga
     3061 ttgcaactcc tatagcaaat aagatatcca agtttccaaa aacggcattt ccagctgcac
     3121 caattactgg gatgttcagc atatcgtcag ctgataaacg catgatcaat cccgctaatg
     3181 gcatcgctgc aactggaagt agcatcgctc ttccaagctt ctgcacattt gatttaacat
     3241 tcatttttat tcctcctcta ttaaatatcc tcatatgtta ctaactcaat caaaggatca
     3301 tttttcactt tttgagcaaa tgctgaatga attaaaaatt cgacttggtg ggtacgagga
     3361 tacacaaaac ttgaaccatt taacaattcc tgatccaaat acgctgcgtg acacatgact
     3421 tcgatcgttt tatcttctct caatatcatg agtaaacttg ccaaatattc ttccacactc
     3481 gtattgattt gtcgtttttt ttcatcagtt tctcctacga catcgaaaca aggctcaaaa
     3541 taatcgacat gacgaacttc atcttttcgt tcaaagttgc tccgaaccgg caaatcatat
     3601 ttttttgcta atgtaacaac tacctcctga ttttcatcca aagtatgagt atgatgatga
     3661 ctgtctaaat gagttggtat gatccccgca tgataaattt tttgaatctg agcattccat
     3721 tcctgataca gttgatcttt gtcaatgtta aatggatgct gataataaga taaggatctg
     3781 aactctccgc tttcgataag agtatcgaca gttttgagga gtggtttgcc acacgtcaat
     3841 gttaaatgga ctcctacacc tagcctaggc atttcctttc ttagtttcac agcatgctcg
     3901 aaacccggca tatttgccat caatgtagtt gatgtcaaaa ttcctcgctg ataagcatcc
     3961 ataatcccat aattcacacc atggctatag ccaaaatcat ccgaattaaa aatcactttt
     4021 cccatttcat tctccttctt ttttctacta aaataggcgc aaccaaataa tcatagtcca
     4081 tttactatgt gtcatttggt tgcgcctata gatagttacg cccttatttt aatagattct
     4141 tgctagatga attgccaaat agatgatttc attttcttga atggcaagtt gaaattgctg
     4201 ttcgatgata tctttaatct ttagtgctgt acaatacgct ttgctatgtt taaatgtttc
     4261 agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga taaatcaagc gtttaaccag
     4321 gaatttgata tgaaccatta agcgatgata agtcagtgat tctttatcat aaacgtttga
     4381 ataggatttt tctagatggg acaaaatcgt ggtcgtcatt tctactattt ttaagctctt
     4441 taaactatta ggctctccta gctcagcatt aatgatatgc atagtgatga atcctacttc
     4501 atttgaccct aactcggttt gatatcgatg attgattcgc tcgaccactt ctactgctat
     4561 atcatattca tttggataaa ggaatctcat ttctggcaac ataattgctg tgacatctct
     4621 atcttctctt tttcgttgaa tagcaaaata aatatgatct gtcaatgtaa tatagatatt
     4681 tgaatctaat ggagaggaga tcctagctgt tatcagttca atcacttctt ccgcaaaatc
     4741 cataaattcg attggaatct gcgagactat ctcactgaat cgtttgatct tttctttaga
     4801 atctaataca aataatttgt cagcatcctc aacatcaaat ggcttactca ctttagcaga
     4861 aaagccaatc cctttcccca tcgcaatttt ttctattcca tcgtcatcga tcaatacagc
     4921 attatgattc agctttttca tcacatacac cgttaattca cctcctctat atgataaaac
     4981 aaaacaactt gcagactcct tcatacatat ttttgtctcc aaacaatttt taaagacaaa
     5041 aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa ttgaatagta gcaccctaca
     5101 taatttacaa aaatagttta acacataata aaagcgttat caatatcttt tttactcgtt
     5161 ttatgataat acttgtatcg aattgatttg gaggtaatta cataaaaaca aaaaaaatat
     5221 agcaactctc tataagaaag ttgctatatt gataattata ccggcggccg gggtcgaacc
     5281 ggcacgtcct caacggacac tggattttga gtccagcgcg tctgccaatt ccgccacgcc
     5341 ggcaaattta aaaataataa ctggggtagc tggattcgaa ccaacgcatg agggagtcaa
     5401 agtcccttgc cttaccgctt ggctataccc caagaataaa gggcgaatga tgggaatcga
     5461 acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatcat
     5521 aaaagatgtt atttaatttc tcaataaaaa actaagatga agacatctta gtcaataccg
     5581 gcggccgggg tcgaaccggc acgccctcaa cgggcactgg attttgagtc cagcgcgtct
     5641 gccaattccg ccacgccggc aagttgattt attagtacta aggcggtaac cggatttgaa
     5701 ccggtgatga aggttttgca gacctctgcc ttaccacttg gctataccgc cattatacta
     5761 ttatcaatct ataaaataaa gaataaatta tag
//
LOCUS       DAZKAS010000147         5790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000147 DAZKAS010000000
VERSION     DAZKAS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.150"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8H7_002435"
     CDS             complement(99..1229)
                     /locus_tag="ACG8H7_002435"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP8984496.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACG8H7_002436"
     CDS             complement(1877..3079)
                     /locus_tag="ACG8H7_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8984497.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACG8H7_002437"
     CDS             complement(3139..3342)
                     /locus_tag="ACG8H7_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFP8984498.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4548)
                     /locus_tag="ACG8H7_002438"
     CDS             complement(3484..4548)
                     /locus_tag="ACG8H7_002438"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP8984499.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACG8H7_002439"
     CDS             complement(4609..4716)
                     /locus_tag="ACG8H7_002439"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984500.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACG8H7_002440"
     CDS             4879..5691
                     /locus_tag="ACG8H7_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984501.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAZKAS010000148         5785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000148 DAZKAS010000000
VERSION     DAZKAS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.151"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..936
                     /locus_tag="ACG8H7_002441"
     CDS             97..936
                     /locus_tag="ACG8H7_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFP8984502.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            1087..4104
                     /locus_tag="ACG8H7_002442"
     CDS             1087..4104
                     /locus_tag="ACG8H7_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8984503.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            4168..5631
                     /locus_tag="ACG8H7_002443"
     CDS             4168..5631
                     /locus_tag="ACG8H7_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8984504.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
BASE COUNT         2211 a         1003 c         1200 g         1371 t
ORIGIN      
        1 aaataaaaaa atctacaaaa aatatatcga ctgatagaca taagtttggt actatgtttt
       61 tgtaaataaa tttattcaaa tcggaggcga aaaacaatga aaatgttgaa agtattggca
      121 ctaggaatcg tagtcggagg agttagtctt gctgtgggaa cgaatgctga agcagcagaa
      181 aacaaagttt ggacacctcg ttcggcggaa caaatcaaag cagaaattaa agggaatgaa
      241 tacacaattg tctggggaga taccctaagc gcaatcagtc aagcaacaaa tattactgtc
      301 caaaaattag cggacatgaa taagattaca aatgtggatt taatctatgc aggaaataaa
      361 ttagtattta atggcaatgt agcaagtgta caaaacagcg caggtgaaac tgtggctcaa
      421 acagtgattc aagagaaaga caaagttgat ccgaatcaac cagtaggaca aggaacgact
      481 tcaaatacac aaacgacacc aaatacaacg acaccaacaa caccagctgg agatcaaaca
      541 ggaacaactg gtggtacaac aacgccaagt acaccagaaa acaatggtgg cactactcaa
      601 acacctacac aaccaggtaa tggtggaaat actggaggag aaacaactcc ttctaaccca
      661 agcacaggtg gaggaacaac aacgcctgaa gaaccaagca atcctggtgg aggcgatcaa
      721 ggcggaaatg gtggcacaac gacacctgaa gagccaacaa ttgtagttgg agagattggt
      781 aatagtggaa aggtatttaa cactgtagat gaagcctatg attgggctaa tgcagaggtt
      841 agcgatccaa attcgcagtg gtacaacaaa ggttttatgg tggatgttat tatttacagt
      901 gacgggtcac aaaaacacac tgtagatttc tactaaaaat aaaagaaaag taagtggttg
      961 atgaagacta gaattataag ttctagtctt tttttgtttt gaggaggata aaaatgtttc
     1021 ggtgtcgaag tccataagaa attactttag aagactgaaa aataaaatga aaggaacgaa
     1081 aaaaatatgt ttaaaagaat ggcaacttgg ttggcaattt tcgttacatt tctcaatccc
     1141 atgttaggat ctgtaacggc atttgcagaa acgataaatg aatcaaattc tactgaagaa
     1201 gtaatgaaga ctgaagaatc aaaaagtagt gaagaagcga tcagtactga aaccacaagt
     1261 gaaacaacag aagagacaac tacgcaggac acgacagaag atgtttctcc gcccaatgta
     1321 atgactcaag aggtccctga aggttatgag aaatacttta taaccattga agatattcaa
     1381 aaagcgactt ctttttctga acaagaacga ttggcgcaag aatctgaaga atggcaagca
     1441 atcttggaaa agaatgcaca gacacgtgct accaatctaa cattgactga aaatggtcgt
     1501 aaagatgtga atatgactcg aattactcct gaaggaacaa tgtatactca tgattacatg
     1561 gttcgctggc aaattgacgg agaagatgtt ttttgcattc aagaaggcgc aatcacgagt
     1621 gcgggtattc aatatgatgt gacaccactc gaaaatttaa ttacgacaag tgctattaga
     1681 aaaaagattt ctttaattgg ttattttggc tactattcta gtggttcaat ggataactat
     1741 gtattagcac aaacatatat ttgggaagtt ttaggtggtt catttgttaa ttatggaact
     1801 attggacaat ctaattatga agctttcaaa cagactgtag atagaaaaat aaacaattta
     1861 cttgaagatc cttcttttga tagccaaaca gtgacggtta aagtagggga agaagtagaa
     1921 gttaaggata caaaaggtgt gttttctcaa tgggatgggt cggttgttga aaacacagct
     1981 aacgttcaag taagaaaatc aggaaatagt ttgtttataa aagcaaatca aaactcaaat
     2041 gacggacgaa tttctgtttc ttcactggat ccaaattcta gaggggtagg ttcaagtttt
     2101 gcctatgtag atcctgttgc tcaaaatcta ggaaagcttt atgtgccaga tcctaataga
     2161 gctggactaa ctattaaagt tctaaaaaat ggcgacttca aactaaccaa actagacgct
     2221 gtcacaggga aaaaagttcc aaatgtgacg tatgaaatca aacgtcatga tacgggagaa
     2281 acaaaacaat atacaacgga tgccaaaggg gaaatcgcct taaaagaaat ccctgcattc
     2341 tatgaaaaag acggaaaata tgttcaaaat aaattggatg tcaaagaagt caaagcacca
     2401 gctgggtata ttctagacaa acaaactcat acgattacga ttgaaccaaa caaagaagtg
     2461 acgttaactg caaaaaatga cttacaacag ttcattccag aaggaaagaa agtcaaagaa
     2521 gtctataatg cggaagaatc ctggaatcaa ggcaaacctg tttatgatac cgttcctgca
     2581 gaaaatatcg aatttgatat tatcgccaaa acagatatta ctgcgccaga taaagaaaca
     2641 gtccttgtga aaaaaggaga agtcttggat tcagtaaaaa ctgacaaaga cggacactgg
     2701 aaggcaaaca aaaaattcta caatggcgat caaaacaaat accaattgat tgaaaaaaat
     2761 gtgccagaca actaccgtca accttctgaa gaacaaacca cgttctctgt ggcctatggc
     2821 gataacacga cgcctgtgct tacgtttgaa ttagatgtga ttgacaactt gttaaaaacg
     2881 ggtgaagtag acttcaataa agtcgattca atcaatcatc tacaattacc aggagcgaaa
     2941 ttcctgattg aaggtatgac tgcacataat aaaaatgtgt cctttgtgta tgaaacaaaa
     3001 gaagaaccag aaaaactaaa attaccagac ggtacgtata aatttacgga aattgtgttc
     3061 cctgagcatt atgggcaagc gtcaggacaa acagaaacga ttttagtaac ggtcaaagac
     3121 ggagaagtac aaccaattac tgtgaaaaac gaagaaatcg tggttccaga agaagaccaa
     3181 ccagaaattt ctactttgtt tgtgacaacg acaggtggaa aagatttcaa cccgaaaaaa
     3241 gaccaagaat tagtggataa agtgtcccaa gtattcccta aatctgcggt tgggacagag
     3301 aaatattggg tgacgcaatt ccataaaatt actgccgatg gaaaagtcac tgtattagat
     3361 acggtaaaag aaacacgaaa agtgaccgat gaaaaagaaa catttgatgt gacgttcaac
     3421 tacaaagcag acatggtttc agacggtgac acgattgtcg caacgcacat tgtctataac
     3481 gacaaagaag aggaagaaga attcgctcgt cattttgatt taaccaataa agatcaaaca
     3541 gtgactgcga aagaaccaaa aatcgaaaca cgctttatta cagtgaatgg cgaccaaaca
     3601 tttgatccag acaaagacca agagttgatc gatcatgtat cgcaagaatt tccagaatct
     3661 acggtagggg aaaccaaata ctgggtgact caattccata agattgataa aaatggaaaa
     3721 gatacagtcg ttggtacggt agaaactgca cgtaaagttt ctaaagcgtc tgaagagttt
     3781 aaagtatcct ttgactacaa agcaggcacg ttaaaagacg gggagaaatt ggtcgcaact
     3841 catattgtct ataaggataa agaccataaa gaggaagaag cacgtcactt tgatttgaag
     3901 aatgaaaaac aaacattgac agcaaaaggc attaaaaaag aaacgccgac aacccctgtc
     3961 aaacaaacgc cgacgagcac accaaaacaa agcttaccaa aaacaggaga aacagtgaac
     4021 gatgtgttaa ttgcattggg actgtttctt tttgttggtg tgggggctgt tctggttcgt
     4081 cgtaaaatga aacaaagcaa ttaaagtatg actagagaca gcaatcagct gtctctagat
     4141 gattgattga agaatggagg caatcttgtg aaaaaaacga gtaaatgttt atctgctttg
     4201 ttattagtag gtcaagtatt tgggtatacg ccagcggttt tggctaatga acaaggcaac
     4261 aatcaactga caaattcgca atctgcaacg atcactgaag aaacgacaac aaattccact
     4321 ggagaagaaa atcaaccaga aacaccgact gaagatacaa tagatacacc agataatcca
     4381 gaaatgaacg ctccaccaac aggttctgaa gaacaaccag aaacaccgaa tcaaccagac
     4441 aaaccagaag acaatcaaca acctgaggag gaaccgtcag aagaaccaaa tgagcaacct
     4501 tctgatgaaa gctcacagac tcctcaaaaa ccaagtaccg aaacagaagc ggaaccgcca
     4561 acaaaaacgg aacgcccaca aaaggcacct gaacagagtg tgactgtacc tatgaatgac
     4621 gtaccgcagg cacaatcaat cggttcagaa gagacaaaaa actaccacca agcaccaagc
     4681 gcaccgatac aagaattagt cccttccagc acacaagata acgaagggac tattcatttt
     4741 gaaaaagacg cgtctgtaga aacgtttatc cgtaaaatcg gtgaatctgc tcgtaaaatt
     4801 ggacaagaaa atgatttata cgcttctgtt atgatcgcgc aagccatttt agaatccgca
     4861 agcggacaaa gtcaattggc tcaggcacca aactacaatc tatttggaat caaaggcaca
     4921 cataacggaa agggcgtctc ttttgcgaca caagaagatt taggcaatgg gacactttat
     4981 acgacccaag caacttttcg gcaatatgag agctatgaag aaagcttaaa agactatgcc
     5041 aaactgttaa aagaaggctt agttggaaat acctcttttt atgatggtgt ttggaaaagt
     5101 aaggcaaaaa cctatcagga agcgactaaa tttttaacag gaagatacgc aacagacact
     5161 agctacgata aaaaattaaa tgggttaatc gaaacctatg acttaatcaa atatgataaa
     5221 gaagtagctg gtccacaact aagcaaagaa ggctatatgg tacctctaaa aaattattca
     5281 atttctagtt ccttcggttg gcgaggagaa gaatttcata gaggattaga catggcagcc
     5341 aatcaaggag aaccaatcta tgcgagcaag gcaggaacgg tcatagaagc ttcgtatcat
     5401 actttgtcag gaaattatgt cacaattgag catgaagacg gaagtgtggc tctttatgcg
     5461 caccaacagg aatattgcgt gaacgtagga caaacagttg aacaaggaca aattattggt
     5521 tatgtgggtt caacaggaaa tagtacagga ccacatttac atttagaact tgccttagat
     5581 ggtagtttaa caaaagacaa attaatcaac ccagaaacag tcttattcta aagaataagg
     5641 ctgtttctga taaggagtgg tatagaaaat gtggttactt tttaaatatt tgatatattt
     5701 gtatctctca ttttcgtttt ttaatttgaa atctcgtatc tatctagaac gtagtttatt
     5761 aaatatagta ttaggaatat ttttt
//
LOCUS       DAZKAS010000149         5782 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000149 DAZKAS010000000
VERSION     DAZKAS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.152"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8H7_002444"
     CDS             <1..888
                     /locus_tag="ACG8H7_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFP8984505.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(904..1170)
                     /locus_tag="ACG8H7_002445"
     CDS             complement(904..1170)
                     /locus_tag="ACG8H7_002445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984506.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFLVN"
     gene            complement(1186..1836)
                     /locus_tag="ACG8H7_002446"
     CDS             complement(1186..1836)
                     /locus_tag="ACG8H7_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984507.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(1847..3019)
                     /locus_tag="ACG8H7_002447"
     CDS             complement(1847..3019)
                     /locus_tag="ACG8H7_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFP8984508.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(3038..3910)
                     /locus_tag="ACG8H7_002448"
     CDS             complement(3038..3910)
                     /locus_tag="ACG8H7_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFP8984509.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3932..4852)
                     /locus_tag="ACG8H7_002449"
     CDS             complement(3932..4852)
                     /locus_tag="ACG8H7_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984510.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(4852..5121)
                     /locus_tag="ACG8H7_002450"
     CDS             complement(4852..5121)
                     /locus_tag="ACG8H7_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984511.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(5118..5498)
                     /locus_tag="ACG8H7_002451"
     CDS             complement(5118..5498)
                     /locus_tag="ACG8H7_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP8984512.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1676 a         1182 c          946 g         1978 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc accaaaaagg caagtgcttc tttgtaggca ttttgactgt atttttttga
      961 taaagcatga caaatttctt gataaaatag ttgactgcct accgaatacc gattattgtg
     1021 tatccgccac aaaacgaaca cactgatttg aatcgacggg cgtaaaaaag aaaaattctt
     1081 tccataagaa aaagaacgtc cttcttgggt ttcttttgct tgttctgttg gtacttcttg
     1141 tacctctctt attgattctt gtctctccat aaccttctcc ttttcttatt cttctaaaag
     1201 ctgttgatta aagctacttc ccaaattttg cataaacgtg attttctttg acgctggatc
     1261 aaaggttacg tcaaaaatat tcatggcatt attccaggaa ctatcaccaa ttttattgcg
     1321 atacgacacc acaacgagcc cttggatagt cgctttttgt gtgcttttta aataaacaga
     1381 acatttctct aattcagaag acaccccgtt tgaatcacca taggaaacgc cttctttgaa
     1441 atattgcttc atcagtttgt cagaaagata tggtgcacaa ttgttttttc gttcttgaaa
     1501 agaatcaggc tcataattaa acagaacctc gaacgcttct gtcactgttt tttgaaaggt
     1561 ttgaatgttt cctttttctg ttgaatcatt gtttttcaat tcttcatttg ctttttttaa
     1621 cgtcgtgacc tcgctttcta cttgttgaag ttgttctttg agtgacgtta caaggtcttc
     1681 ctgtcctttc ttctcttctt ggaacttgtt tatctccacc gtcagtttct cttcctttgc
     1741 tgttagctgt agcttttcat gaataagaaa aaatgtactg cctacaccaa ataagaacaa
     1801 tccgcctaca caaacataaa taaatcgttt cttcatttga cacctcttat ttctttatcg
     1861 gtcgagcaaa acaaatatca ccaccattaa gcaaataccc aaaggataaa ttgacttgcc
     1921 cttcacctac accgttagta ttcattcccc cttgttcgat tactttcgta gaaagtccat
     1981 gccaatcttc cgctaaaaca gctgtgtgtc catcatttcc cacaccagaa cctaagttca
     2041 caatgataat atcgccagct tttgcctcgc tctcaggtat ttcttgtaag tattgcttac
     2101 ttcctcttgc gtcgctcgtc attgagccgg taaaccaccc aacgttcgca ggcgttttat
     2161 accctgcttt cgttaaaaca agccacacaa agctcgaaca atcggcatag ccatttttat
     2221 ctggattttc gacactacca aattgtgccc gttgcggttg tgaataatga aaataaccaa
     2281 tcagactttt tgctacgttt aatacattac tatcggaaga actgcttgtt cctgttgttt
     2341 ctagacaata atcggtcaag tctttacttc ctgcgtttgc cccattttct aatgtaattg
     2401 aagcgacggg atcactgtca ttctttccta gttttgcgta ccattgattc gcaaaagtaa
     2461 tacgattccc catcattggt atccctgcac gctcaaattt cacttgccac tgttctgttg
     2521 cttcctctac gctttttgct tgtaatgcaa cttgtgaagc tttttctgtt gtgtttagtt
     2581 catgaatcaa aaactcaact tgtacaccta aatctgacca ctctttgttt cgctctttgg
     2641 caaaattttt caaagcagtc gttcgccctg ccgtccattg tgcaatccca tgcccagata
     2701 atggatcgtt tggtcgttca atcgctgtgg gactcatact cgactcttgt tgaaaattgc
     2761 ctaacatacc cgctagaccc tctaatgtcg tatttggaat atgtttttgt acttcctttg
     2821 caatttgaag cccgtttgtc tccatgcctt ggttcgttaa tgagatagta cttgcgacaa
     2881 tttcggttgg actacagtcg atttcttcct cttcagaacc actactaatc aaaagtatca
     2941 taaacagaaa accaaacata catagtaata gtgtgggtag acaaccgaac ccacaccata
     3001 aaagaagttt tttcttcatg tttgatcctt cattcgctta gttcattttt tgtgttgctt
     3061 ttcttttgcg ccaaaaagca aaaccgcttc ctgaagccaa taatccacca agtgctgtcg
     3121 caacgagaga cacttcttcg ccagtcttag gcaatacttt catttttcca tcagaccctt
     3181 tcaccgcaat atttccgctt ggaagttgtt cgaccttgtc tgaaatgggc gtcaagccat
     3241 ttcctgattc tttataagcg acaccatcaa cgacggagac aattttttct cctgaagctg
     3301 tttcaattgg tgtacttggt gttggaatat gggttaattc agacgcatta ttggtttcaa
     3361 tttgaacatt ttgtccacct gtttgttggc tgccaatact tccatctggt tttactacga
     3421 cgtcctgtac tggaggcgta gtgttcgtcc ccccattatt ggtaggtggt gtcgttggag
     3481 gagtcgtatt tccttcattg cctcctggat tcgacggctt ttctggtgtc tctgtattac
     3541 caccatttcc tgggttttcc tcaacgggtg gaacttcagg tgttccttcc cctccgccag
     3601 tatctggatt ttctggtact tcaggcgttt ctgtgcctcc acctgtatct ggattttctt
     3661 ccgttggtgg aatctctggg gtttcaggag tttctggtat ttcgggaatt tctggggttt
     3721 cttctcctcc atctgttggt ggtaaaacag gttcttcagg aacttcaggt gtttcttccg
     3781 ttggtggaat aatttctgtc ggtggtacac ttggatcacc tggtgacgta gtatctgcat
     3841 acgcactggt tcctaccata cctagcaata cagttgcact aaataaactt cctaaaatca
     3901 tttttttcat gtgtttttcc tcttctctta tttattgatt tatttcaaaa tcatctagtt
     3961 cgggaagcgt ggaaactaga taattttttt cctgttcttc caattgaaaa gacacttttt
     4021 ttgtttcttg tgtctttttc tcggtttcca atgtaatgac atacgcaatt tgccaatgct
     4081 tgccttcttt cttcatttcc caggataaaa tacttttgac tttttccttt tgaggactga
     4141 tttgtttcgc aactttttcc gttagaaacg attgaagctt tttcctatac cgttcttctg
     4201 ccatttcacc cgaataataa ttcgtaataa aataacgact aaatgtatca atttgtggtt
     4261 gttttgtcgc aagttcttcc gcaaatgcaa tgcgttcttg taactggtgc tcctttttcg
     4321 ttagttgttc aatctctctt tgcgttgaaa gttgcataat aaaaagacca caagatacaa
     4381 cgacgataaa tccaaacaaa agccattgaa aagacatttt tttcttctga agagagccgt
     4441 tgctttgtgt aaacgctgtt tctacagaaa gtggtgtttt cttttccttt ttgatttttg
     4501 ttggtcgacg ttcgttcttt tggtctttct ttacttgata aaacttcggt aattcggaat
     4561 ttaaggtttg aataaaggtt tgtgcttctt cacttaattg cccattttct gttaaagatt
     4621 gttcaatcaa tgaaatcaaa tgaaactctt ctactggaaa atcatacgag ccttcaaata
     4681 ctggaatttc atggttttct gtgtagattt ccaaggtaca ccagccaatt tccgctcgtg
     4741 ccaatagctc ttcattttct cttttcaatt gaataaatgc cttatctaat gcaatttttt
     4801 tatctgttag taaagacatg ttatcaactt ctgtaaatcg aatcatcatc attagtcaaa
     4861 cacttcttcc accacttcta ccgcatacac cacttgaata gggttattta aagaaaattt
     4921 cacccgatat tcttgattgt tcaaactgac gatatagcta tcctcatctt cagcataata
     4981 ggtatattcg ttgcctttga cttgaaaata tttttggatc gcttgtcttt gttgccattc
     5041 gccaatcttt ggaaaatagg cgaaatgata gccaaataaa acgcctatcc ctaataaaac
     5101 aattcctgtt actataatca tctggtttcc tcctgtacct gtttatcttc tattgactct
     5161 tttgtcggta agcaataaag aaccagtaac acaagagagc caatcgttgg gacaaaatag
     5221 aaaaaaataa atgcccaatg aaaacctgcg tctcgtaaac gtcgaacgct gagagaaaga
     5281 gacgggacaa gaactacgaa acaatacaca atcaatacgc ctatcaaaca accaattatt
     5341 gtttcagaag cattcaacgc tcctgctagt ccaatcgcta agcctaggcc aaacccaatg
     5401 agggcattca ttaacgcaac gagccaatac tcttttcttg tggctttccc tttgtaatta
     5461 aagctattct gccaaaattt tttataattt gtgatcatat ctttgtcctt tctaatgttt
     5521 atttttgatt ctagtttctt ctgttttact aacaatgaaa cttgccataa aaaaggtggc
     5581 caatacacca ccagctagaa atccacttac aaataacaaa attttttcct cctttctaaa
     5641 aaatttcgcc ataaaaaggc aaagtatgaa cacgataaat accatgtaca tactttgcct
     5701 ttttatgcaa tatatgttaa ctctccaagc aattactgat ttttaaattc tttataaata
     5761 aaataaaaat cgtttgagta aa
//
LOCUS       DAZKAS010000150         5726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000150 DAZKAS010000000
VERSION     DAZKAS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.153"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="ACG8H7_002452"
     CDS             complement(1778..2443)
                     /locus_tag="ACG8H7_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984513.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2569..4605
                     /locus_tag="ACG8H7_002453"
     CDS             2569..4605
                     /locus_tag="ACG8H7_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFP8984514.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="ACG8H7_002454"
     CDS             4609..5247
                     /locus_tag="ACG8H7_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984515.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="ACG8H7_002455"
     CDS             5275..5703
                     /locus_tag="ACG8H7_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984516.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1888 a          867 c         1046 g         1925 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaat
//
LOCUS       DAZKAS010000151         5677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000151 DAZKAS010000000
VERSION     DAZKAS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.154"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1190)
                     /gene="mnmA"
                     /locus_tag="ACG8H7_002456"
     CDS             complement(69..1190)
                     /gene="mnmA"
                     /locus_tag="ACG8H7_002456"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP8984517.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            1594..2193
                     /locus_tag="ACG8H7_002457"
     CDS             1594..2193
                     /locus_tag="ACG8H7_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8984518.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2331..3617)
                     /gene="efmA"
                     /locus_tag="ACG8H7_002458"
     CDS             complement(2331..3617)
                     /gene="efmA"
                     /locus_tag="ACG8H7_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFP8984519.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3888..4232)
                     /locus_tag="ACG8H7_002459"
     CDS             complement(3888..4232)
                     /locus_tag="ACG8H7_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFP8984520.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(4369..5514)
                     /locus_tag="ACG8H7_002460"
     CDS             complement(4369..5514)
                     /locus_tag="ACG8H7_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFP8984521.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT         1665 a         1231 c          991 g         1790 t
ORIGIN      
        1 gctatgctat ccaaaagaaa aggtgtttct tttgttagcc atagctgttt tgattcattt
       61 aatatcgctt aaacgtattg taaaactttt gtttcttgat atgcgtggtc gatcaaacca
      121 ccacccagac attccattcc atcataaaat acaactgctt gtccaggagt gattgcacgt
      181 acaggttcgt cgaatacgac ttctgcacga ttatcatcca ataagcgaac tgtcacagga
      241 acgtcttgct gacgataacg gaatttcgct gtacacttga attcttttgg cattggttca
      301 ttcgtcgtaa agtggatttc acttgcatcc aagcttgtgg catacaatgc aggatgatgg
      361 aacccttgcc ccacatataa tgtgtttgtc gctagatctt ttcctacaac aaaccagggt
      421 tcttgcgaat ctcctccgcc accgatccct agtccttgac gttgaccaat cgtataatac
      481 atcaagccag catgttgtcc tttgacttca ccatcaagag tgaccatatt tccttttttt
      541 gctggcagat agttgctcaa aaattgtttg aagttctttt caccaataaa gcagattccc
      601 gttgaatctt ttttcttagc agtcgcaagg cctgcgcgtt cagcaattgc ccgtacttct
      661 gatttttcca tgccgcctag agggaacatc gtttttgaaa gctgttcttg tgataattgg
      721 ctaaggaagt aagtttgatc tttattattg tctactccac gaagcatgtg gaccacgccg
      781 ttttcatcgc gagtgacttg cgcataatgt ccagtagcta cataatcggc gccaagatcc
      841 attgcataat ccaagaatgc tttgaatttg atttctttgt tgcacatcac gtctgggtta
      901 ggtgtacgtc ctgcgcggta ttcggctagg aaatattcaa atacacgatc ccaatattct
      961 ttttcaaaat tcacagagta ataaggaatc ccgatttgag cagctacttt cgctacatcc
     1021 ttataatctt ccgtggccgt gcatacaccg ttttcgtccg tgtcatccca atttttcatg
     1081 aatattccga tcacgtcgta tccttgttcc tttaacaaaa gtgctgttac agatgagtcg
     1141 acaccgccgc tcatccctac gacaacgcgt gttttgctgt tgtctgtcat gatatcacca
     1201 tcatttcaat tagattctga aacatctacg agttgaaggt cgcaactttt cagtatttta
     1261 cattatacgt catatttctg aaaatttcat cccttaatta tcaagtcgta aaaatactgt
     1321 tctactggac ctattcagta cacaaacatg atagtcaacc aaaaaatcac tcatcttttg
     1381 agcattcgat ataaaaaatt ggcatactca taaaaagggc cagcctttcg gcaataagga
     1441 ataattttat aaattgtgaa aaactatttt tcaaaattgc ttcttatcgc ttaaagctgc
     1501 acgtctcatt tacaaccttt tctttttaga tgttatgagt tgctcagata agtgattttg
     1561 tttactctaa gaaaaaaggg aggcaaaaga tacatgaatt actttatcag tgatatgcac
     1621 tttttccatg aacgcttatt aggaaaaaac gactttgctc cgcggctctt tgcaaatgtg
     1681 gatgaaatga accaacagtt gatcactagc tggaatgcag taataaaaga aaccgaccat
     1741 gtttatcatc taggcgacct tgctatgcat ccgcaatatg aaaaagcaaa tgcagaaatt
     1801 ttggaactgg tcactcaact gcaaggaaca atccacttga tcaaagggaa ccatgacagt
     1861 cgcgcttttt tccgttattt agaaaaaaat gatcctaggc ttactgacgg aagtcccaaa
     1921 ttttattttt atgatgtagg tgccatcgtt aaattcgacc atcaacaata ttacttgact
     1981 cattatccga tgttgctcgg gcctaacgac actatccgca atcttcatgg acatatccat
     2041 cattacagcg tacctatcga gaacgatgtc aatgtaggcg tagacgcacc ggaaagagaa
     2101 ctcttaaagg aaaaactacc atttggcact ccgttatctg agcaacaaat caaagaaatc
     2161 tatgaagcaa agaggaagta cgataggtcg tgaaaaaact gccctatatc aagtattaaa
     2221 caaataaaga taattactca tatttttcat ttgagtaggc ttattgccac aggaacatta
     2281 ggatcagaaa tggagttgta acaagacttc tgtcacaact ccattccata ctattcgtct
     2341 ttttgctcat ggtgttcttt taccagtttt ttctgtaatt ctttatcata atttcgggca
     2401 gctgacgtta aaaataaaac gattccgcat aataaaatcc cgattccgga aaacaagaac
     2461 attttttcta cccctaaacg atcagctaat ggtcctgcaa ataataatcc aattggacct
     2521 gtcaggttca tcaaagagtt gaagttccct aagacacgac ctaaaacatt cgattcataa
     2581 ctctgctgga tcattgccat cagcagtgta ttgaaatatg gcgttgcaca acctgctcct
     2641 gcattcaaga taagaaagta caagaaccct tgactatcgt ttggaaccaa tccgcttgca
     2701 ccgatcgtca ggcccataat gatgtaggct ataataatag gtttcattcg gtctttccat
     2761 gttcctataa aactgatcag tgcaccgcct aagagcattc cgactgcgta gactacttcc
     2821 actgctcccg cctgtccaac ggaaccacca aagtaatcta gcgtcatcaa tggatacaga
     2881 ctgattgctg gcatgaacaa cagcatcaca aatgcaccat tgatcgttat gtaccataac
     2941 cctttattat ccatcaattg ctgcagccca aattttgcat ctttaaaaat agtaagcgga
     3001 acttccaata tttcaggaga aacttttggg atcttcacaa aaatcaataa gccgacacca
     3061 aaaactgccc ccaatacatc taataaaata aggtaattga caggaacgac tgaaaacaaa
     3121 gccgcaccta gagctggcgc aatgatgaaa ttcgctgatt ggaccattcc taactgtcca
     3181 ttcgtccttg ttatgtgaga agatggaacg attgtcggca taatcgactg gatcgttggc
     3241 atttggaatg tttgtgcgac cgaacgcata aacaaagaca caaaaactag ccaaatcggg
     3301 aaggattctg aaatcgttcc gactactgct aaaataagcg cgaaaatcgc tacaataata
     3361 tccgtgacaa tcaaaagagc ttttttatcc cagcgatcca ctagcggtcc tacaaatggg
     3421 cttagtaaaa ccatgggaat cattcctagt aaagtagcaa aactcaaaat tgtcgccgaa
     3481 ccggtttctc ttgtcaaata ccagatgatc gcatattgga cgaccatact agtgattcct
     3541 gacaaaaact gtcccgataa aaagaacaaa taatttctct tccaattcgt taaaacgacc
     3601 gactgttcat tttccataaa catccctctt ttctttttca ttttgtatag cgtttgcaca
     3661 acaatcatta tacgggattc ttttttcaaa aaataccttt ttccctaaat aacaatcgtt
     3721 tcaatttaaa tttctttact tagaaacaat aaatcagcaa aatattaaaa ataacaacat
     3781 gtgttaaatt taaaaataaa aaagacaact aatttgcgtc gtccttacaa agttagtcat
     3841 ctaacaatag gccgtttcaa aactagttgt ctttttatat ccacagatca ttcgactttt
     3901 tcgaataacc cacggctaat catcccatcc atcagaaaat aaaatttatc ttggctcatt
     3961 ttctgttttg gatctttgaa aatattgact gctttcaatc catttgctgt agtgatcgac
     4021 attttcaatg tttgaatatc ttttgataca gtgattttta atttgaatcc ttctttgctt
     4081 tgcggcgtgg catctaatgg tcggatcaat tgataattgc ctccgtttgt ttctgtaaat
     4141 ccattatcgc gcaatgtata tctttttgca tcaggatgca aacgatagct aattggtgcg
     4201 cccagtaccg ttgctgttgt ttcaaatgcc atgattttca cctcgataaa attagtttct
     4261 ctttattata aaagtttatc ctgattttta ctaggttttt cagttaagtt ccctaaaaaa
     4321 tgaaggcgtt ctctcagaca tctgttcata taagaatgac ttcactagtt attttttgac
     4381 agcacgaact aattgatcaa ggaaatactg gatgtcgtct tcggtatttc ccaaaccaaa
     4441 gctgatacgg atggattctt ttagtatcgg tgtatcttct ccatacattg cttccaatac
     4501 gtgtgaaggc tcgatatttc ctgctgtaca agctgaacca gtagaaatag cgaccccatt
     4561 aagatcaagc ttcatcagta gcaaatcatt tgcttcgcca attaagcgaa gattcaacac
     4621 atgagggagt ttattcgtta aatcaccatt tacttgataa gggatttcgt ttgcttctaa
     4681 tgtatcaaga atcaattgct ggaatccttt atatttgttg ttacgtgctt cacgttcttc
     4741 aggtgacagc agttcaactg cttttgccat accccagatc cctgctaaat tttctgtacc
     4801 tgcacgccgc ttttcttcct gctccccgcc taaaagcaat gcagggatat ttactccgtc
     4861 tcgtttatat aagaaaccga cgccttttgg cccattgatt ttatgagcag agatacttaa
     4921 taaatcgatt cctaattctt ctggatggat cgtttggttt ccgtacgctt gtaccgcatc
     4981 tgtatggaaa taagctggat gatccgctaa aagttcaccg atttctttga ttggcatcaa
     5041 atttccgatt tcattgttcc cgtacatcac agagaccaag atcgtatctt ctcgtaacgc
     5101 ttttttgacc tcttcgatcg acagattccc tttttcatct acaggcagat atgtaacgtc
     5161 aaaccccttt gtctctaaat atttcatggt attaagaaca gcaggatgtt cgatagctgt
     5221 agtgattaga tgtctgcctt ctttttgacg cgagaacgct gtttctaaga tagctgtatt
     5281 atccccttct gttcccccac tgttaaagat gatctcgtga ggttttgctt gcagactgtc
     5341 tgcaatgatt tgtcttgctg tttccaattt ttcatgcgcg tggcgaccaa atccatgtat
     5401 gcttgaagga tttccaaacg tgtcttgcat gattgccgtc atcgcttcaa ttacttgtgg
     5461 atgcatgggg gtagttgccg catgatccag ataaatagtt ttcacttcat tcatcccttc
     5521 caaattattg ctaggcacat tctagcataa aatagcgaac aaaccaaaga agagctcatt
     5581 tttgtctaga ggacgtttca tgggcataga aaaaaggagc tgtgacaaaa gtcttgtcgc
     5641 agctccttga agctgctcac ttctgtcaca acttctt
//
LOCUS       DAZKAS010000152         5645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000152 DAZKAS010000000
VERSION     DAZKAS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.155"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..1150
                     /locus_tag="ACG8H7_002461"
     CDS             158..1150
                     /locus_tag="ACG8H7_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8984522.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1210..2631
                     /locus_tag="ACG8H7_002462"
     CDS             1210..2631
                     /locus_tag="ACG8H7_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP8984523.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2624..4123
                     /locus_tag="ACG8H7_002463"
     CDS             2624..4123
                     /locus_tag="ACG8H7_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFP8984524.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4162..5556
                     /gene="uxaC"
                     /locus_tag="ACG8H7_002464"
     CDS             4162..5556
                     /gene="uxaC"
                     /locus_tag="ACG8H7_002464"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP8984525.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1851 a         1048 c         1161 g         1585 t
ORIGIN      
        1 cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg
       61 tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg
      121 cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatatcgc
      181 taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat
      241 aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa
      301 ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag
      361 tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt
      421 attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat
      481 caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt
      541 catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga
      601 tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta
      661 cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc
      721 gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa
      781 tctagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata
      841 cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat
      901 tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat
      961 cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa
     1021 gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag
     1081 gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa
     1141 ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag
     1201 gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa
     1261 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
     1321 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
     1381 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
     1441 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
     1501 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
     1561 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
     1621 ccacagagct tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
     1681 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
     1741 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
     1801 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
     1861 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
     1921 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
     1981 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
     2041 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
     2101 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
     2161 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
     2221 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
     2281 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
     2341 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
     2401 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
     2461 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
     2521 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
     2581 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
     2641 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
     2701 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
     2761 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
     2821 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
     2881 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
     2941 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
     3001 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
     3061 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
     3121 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
     3181 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
     3241 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
     3301 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
     3361 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
     3421 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
     3481 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
     3541 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
     3601 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
     3661 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
     3721 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
     3781 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
     3841 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
     3901 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
     3961 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
     4021 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
     4081 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
     4141 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
     4201 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
     4261 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
     4321 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
     4381 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
     4441 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
     4501 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
     4561 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
     4621 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
     4681 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
     4741 aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
     4801 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
     4861 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
     4921 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcttaca
     4981 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
     5041 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
     5101 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
     5161 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
     5221 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
     5281 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
     5341 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
     5401 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
     5461 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
     5521 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
     5581 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
     5641 accaa
//
LOCUS       DAZKAS010000153         5614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000153 DAZKAS010000000
VERSION     DAZKAS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.156"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..2008)
                     /locus_tag="ACG8H7_002465"
     CDS             complement(59..2008)
                     /locus_tag="ACG8H7_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8984526.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="ACG8H7_002466"
     CDS             complement(2050..3108)
                     /locus_tag="ACG8H7_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFP8984527.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="ACG8H7_002467"
     CDS             complement(3227..3682)
                     /locus_tag="ACG8H7_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984528.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="ACG8H7_002468"
     CDS             complement(4266..5069)
                     /locus_tag="ACG8H7_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP8984529.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DAZKAS010000154         5508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000154 DAZKAS010000000
VERSION     DAZKAS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.158"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..2124
                     /locus_tag="ACG8H7_002469"
     CDS             64..2124
                     /locus_tag="ACG8H7_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFP8984530.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2207..2776
                     /locus_tag="ACG8H7_002470"
     CDS             2207..2776
                     /locus_tag="ACG8H7_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8984531.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2951..3757)
                     /locus_tag="ACG8H7_002471"
     CDS             complement(2951..3757)
                     /locus_tag="ACG8H7_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984532.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3895..5409
                     /locus_tag="ACG8H7_002472"
     CDS             3895..5409
                     /locus_tag="ACG8H7_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFP8984533.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1956 a          954 c         1126 g         1472 t
ORIGIN      
        1 tgaaatcatc ttatgaaaat tactacttca tgtttagaca agagtaggga ggactaagaa
       61 taaatgtgtt caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg tttagataag
      121 gaacggtcgt gttctgaaaa acactacgtt aagtttatga tgcgcacgat gctgatcagt
      181 ttatctgtag gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc
      241 gtacgtacct ctgtttctga gaccactgat tcccctatca ctattactca tactttcact
      301 gatgcaaatg gaaaacagct acttcctgca ttgacttcct ataataaagt cagttatcct
      361 ccaacgataa accactactg gttaattcct aacaaaacat tatggacatc ggaaaccgga
      421 agcagtgtgc cgctttctat cttttccgat atgactgatc ctacaaaagg tgatcagtta
      481 aaacagatca ctgatatttt gaatcatgct ttgacagata taaaggacgg tggaagcgca
      541 caagtttctt atgtatatga aaaagattat tcacagtttg aaagaaagaa aatcgatgtt
      601 ctggcaggga caaaagtttc gatatctgat ctggtagaaa ggctgaagaa cgcagatgga
      661 acacaaggag atatgtccaa agtaatttca aacaatggga atatcgtgga tacgactaat
      721 acaggaacat ctactatttc cttgtcctat tttgatccaa ttgctttaaa aacaatgact
      781 gcatctgctg tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa agatcttcac
      841 ttaagaacag gagagagtta cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat
      901 ggaacttcag gaaataaaga agaaatcaaa atgaacggga cagtagatac tacaactccg
      961 ggagaatatc ccgttgtatt aacttatata gactcgaaaa catcaaatgt ggcagaaaca
     1021 acagctgtag tttatgtaga agcatctgct gcttcatcga ctgtcatcac tcattttata
     1081 gatagtcaaa caaataaaga acttcaagca gaaacacata tgagtagtgt aaatcgtatt
     1141 cctgatttgc gcctacctgc tgaattttct gatcgagtgc caaatataga tttatcgact
     1201 tatgaacaaa atgggaaagt cacttctatt tctgattgga tgcaagcaaa cggattagac
     1261 agaaaaagtc actggtcgga gatccttgaa gtacttcact cagaactgat gaataatagg
     1321 aagcaatcag gaggggaaat tacctttact tatgtttata taccagacaa tcgttcatta
     1381 gaaggtcatg atcttattgt tcatccaaac gaaaaaataa acaaagaaga gttgatccaa
     1441 tcagcaacaa attccttagg taaacccgta ccgttaaagg atgtatcggt tacaatcaat
     1501 gaagtaccat tggaggatgg ttatttgacc aaacaaacag gtgattttac gattatgtat
     1561 agttatcatg acccgtattc actccaagaa ctccatgctc agtcactatt acatgtttta
     1621 ccattaaaca aacagataga tcaaaaagaa caaaaaagac gagaagaaag cttgtcagaa
     1681 aaaaaacaac aaatggctca tgcagtttct ccaacaggga aagtaaaaca aacaagaagc
     1741 ctagagttag caagaatatc tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa
     1801 gctcatttgg ccccacaagt ttctggattg gaaaatgaaa ttttagagcc taaaataaaa
     1861 caacaatcag aaaaagaatt tgttgctaaa cagtcggaag atcagaagga atcagatgac
     1921 cagaaggaaa cgaagaaaaa acgcttgtca actattgatt ccaccgatga gcatctgcag
     1981 ccgcaagtac cacttcctag tccgggaaca cccgcacctg gtggaggtat tccttcttct
     2041 gctttaggag atttcatgcg aggtatggca ggaacatggg tatacgctaa tcgaaatgaa
     2101 tttgacgcag atgactggtt ctgatttata tctctatctg ttatagtcaa ataagtagct
     2161 gagtcatatg ttatttggca taatggagac gaggagggat agaaaaatgt tagataaaac
     2221 gttaccttac gcagaaatct ggatgacaag accacttcaa caaaatgttc caatcacgcc
     2281 tttagcggaa ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga
     2341 gattgagact tccgttgggg aatttcaaac gacagatgaa gcacttgagt attttcaaca
     2401 aaactttgct ccttatcctg aagaacttga aagaaggatg ttttttgtag agacaaaaaa
     2461 aggagaaaaa gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc
     2521 ggtttttcat tggttagcag tgaaaccaga ttaccaagga aaagggctcg caaaagcatt
     2581 gactgccaga gtcttgcaag aatttcctac cctccatacg gacggaccaa tctatcttca
     2641 cacacagaca tggagccatc aggcgattgc tctttataaa aaaatgggtt ttacttttat
     2701 tgctgaaaat ctagacggca cacctaatcc agattatgaa aaagttatgc aggttttaag
     2761 taaattagat aaataacgaa atggtgaaat aagtcctcag tattgggata aaaaacgaaa
     2821 tagtcattcc tcatatctta cttttttgag ttaatactat cggataacta atggcatgcc
     2881 atacattcaa aaaagaaggg gcagtgacaa aagtattgtc actgcccctt ctcttgtttt
     2941 ttagcatgtt ttatcgtttg atttttttag gaataaaatt cttgatgaac gtaaattttt
     3001 ggtaatcgtt tcgactatag aggtaaccaa atacactgaa aacaactgct ccaataaaat
     3061 taacaaataa atccttcata gtatccatga ttcccacgtc taagtaacca ttttggatga
     3121 ttgttttatc tgttttacca tctgcatttt gactttcaat gattgtccga gtgatatctg
     3181 gaacatgata gacagtgttt ccgtctttac ttaactccac actattgatt tgctggatga
     3241 tcgtatcttt ttgcatatcg aattgcagta atttatctgc tgaaaattcc acaaactccc
     3301 acataacacc aactgtcatc gaaaaacaga aggaaacaac tgctagtaat aaaggagtca
     3361 agttcatggt ttttgtattt ttattgagca aataaacaag ggaaaagccg acacccccag
     3421 ctaaaaatcc gctgaatgca tgtagaaaag tatcccacat tgaaaaatag ccataaaaat
     3481 cacttaactc tcctaaaact gttgaactaa aaataaaaga taacaaaatg atttctagca
     3541 tatttggaat ctgtatcttt aatatctttt ccagaaaata agggatttca aataaaatta
     3601 atgtcagtaa taaaagcatt gtatttcccc agtgttggtt ccagacctga tccactagca
     3661 taataacgac gatgatacgt aataaaatat agattccgtt tgtgattcta cctgcagttt
     3721 ttgtactgtt cgtaaaaaag gttttcaaat gattcataaa ataactcctt catgtatatc
     3781 cttaattata ctaagaagcg tgtgtttacc aaagctatat gacttaaaga aaaacagagt
     3841 tgatattgtg aaaaaaatca cttaatatag tataaggaga aaagggggat acaaatgaaa
     3901 aaattagttg ctggtttatt aacagccgtt gcacttcttg ctatgagcgc atgcggcgca
     3961 gatcagaaaa atacagcgca gacaagttct agtacgaagg aatctgccga agtatcatca
     4021 ggcgcttctg aacaagtata tacagatcca aaagaaatga aagaatctta tgatgtcgtg
     4081 attattggag ctggtggtgc agggatggca gcagctatcg aagcaaaaga tgctgggttg
     4141 aatccagtaa ttttggaaaa gatgcctgtt gccggaggaa acacatctaa atcatcaagc
     4201 ggtatgaatg cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat
     4261 gccttctatg aagaaacgct aaaaggtggt ggaggaacga atgataaaga aatgcttcga
     4321 tactttgtcg atcactctgc tgatgcggtc gattggctag atcaaaacgg tatcaaactg
     4381 gacaacttaa cgatcactgg agggatgagt gaaaagagga cacatcgtcc gtctgatggc
     4441 tcggctattg gtggatactt agtggagggg ttattgaata acgtccatga gcgggaaatc
     4501 cctatttttg tcaacgcaga tgtgacagac attaagaaaa atggggaaac ggtcaatgaa
     4561 gtgactgtac atgttcaagg agaagaacca aaagaggtca ccggaaaagc aatcgtagtt
     4621 accacaggcg gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc cgatttagct
     4681 gattatatta cgacaaatca agaaggatca actggagatg gtatcaccat gattgaaaaa
     4741 ttaggtggac aaaccgttga tatggataaa atacaaattc atccaactgt gcagcaggat
     4801 gagggggtct tgattggtga ggttgttcgc ggagaaggag caatcttggt cgatcaagag
     4861 gggaaaagat ttgtcaatga aatggataca agggataaag tatctgcagc aattacagca
     4921 ttgcctgaaa aatcagctta tttgattttt gaccaaggcg tgcgtgatcg tgccacagct
     4981 attgaatttt atgaccaaaa aggatatgtc acaactggaa agacaatgga agaattagca
     5041 gaaaaaatcg atttaccaaa agaaacacta gcaaaaacag ttgatggatg gaatacggat
     5101 gttgcggaga aaaaagatgc tcaattcaac cgtgccacag ccatggaaca tgacttatcg
     5161 tctccgaatt attatgcgat caaaatcgca ccaggaatcc attacacgat gggcggtgtg
     5221 aaaatcaata cgaacactga agtattagat aaagaaaacc agccaattag ggggttatat
     5281 gctgctggtg aagtcacagg tggcttacat ggaaacaacc ggatcggtgg aaattcagtt
     5341 gcggagatca ttatttttgg gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa
     5401 aatgaatgaa atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac
     5461 cgagggtcag ctgattgaac actgtttagt gaagaggttg tgaaaaag
//
LOCUS       DAZKAS010000155         5343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000155 DAZKAS010000000
VERSION     DAZKAS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.159"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACG8H7_002473"
     CDS             complement(<1..302)
                     /locus_tag="ACG8H7_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984534.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDS"
     gene            complement(600..1574)
                     /gene="sgrA"
                     /locus_tag="ACG8H7_002474"
     CDS             complement(600..1574)
                     /gene="sgrA"
                     /locus_tag="ACG8H7_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFP8984535.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1770..2609)
                     /locus_tag="ACG8H7_002475"
     CDS             complement(1770..2609)
                     /locus_tag="ACG8H7_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFP8984536.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2794..3681
                     /locus_tag="ACG8H7_002476"
     CDS             2794..3681
                     /locus_tag="ACG8H7_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP8984537.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4265..4960)
                     /locus_tag="ACG8H7_002477"
     CDS             complement(4265..4960)
                     /locus_tag="ACG8H7_002477"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP8984538.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4944..5342)
                     /locus_tag="ACG8H7_002478"
     CDS             complement(4944..5342)
                     /locus_tag="ACG8H7_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP8984539.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1706 a         1081 c          783 g         1773 t
ORIGIN      
        1 gaatccagta aaatataacc aatcgttccc tctaaaagta tagaataaac tttactcagt
       61 aaaaactgag aagaaaactc gacttttatc atcttgtttt ctgaaagaaa agataattta
      121 tattctggat acctttcgca aaacgattct gcttgttgaa tcaccgaatt catcttagcc
      181 agggtgtagt ttagatctaa agcgatatcc ttcaccacat ataatttctc gtctctttca
      241 taacaatagt tcaaaaattg atatacttgc ctttcattga tatcaataaa ttcttcaaac
      301 aaagtacata ctccttttta tctattattt agacttattc cgacagctac atgaataacg
      361 tacaaataga ttcgttactg atttttcaga gatactacca tgcgcagtgt gcattgtcaa
      421 ttattcttgc tcttattttc catttgtgtt cctgttattt cctgtttctc aaatatgtta
      481 gtaaacaaaa atagatctgg caaagctaca acttcgccag gcaatacagc actctcgaat
      541 gaaatattca ctcaaaaatc tatcgtttta ttcaatttta gatctgttgc ttttattctt
      601 tatttcactc gatggaagag agaacgaaaa tagcttgctc ctacaatcaa aatgagtaac
      661 acacccatcc atgtaattag cttgttcaag gttctactac cagtttctgg taaatcgctt
      721 tttctattat ctgtttcttt cgctttttct tgatgatcaa ttccacgaga aacaaaatca
      781 ggctgtttta gcacactttt taggttgctc cctgcatttc tcttttgttc cttagttggt
      841 atgataggta ccaaatcaag ttcattattt acttttcctg tgctgctttc gctgctcgtg
      901 cttcctgtgc tactctcact actcatgctt cctgtgctac tttcactact cgtactctcc
      961 gtactgcttt cgctgctcgt gcttcctgtg ctactttcac tactcgtact ctccgtactg
     1021 ctttcgctgc tcgtgttttc tgtgctactt tcactgctcg tactctctgt actgctttca
     1081 ccgctcgtgt tttctgtact gctttcacta cttgtgctat ccgtactgct tggcggtcca
     1141 acaactgtcc aactgcttgg tttcaaattc tccgtgatcg tcatctgtcc atcataaaaa
     1201 acaatcaaat caactttatc tttactcttg atctcatcaa tcgatagttc tgcagaaatc
     1261 attattgtat actcaatacc agcggtccat tgtacgtctt ttaaataaac ttccccaata
     1321 actttttctg aacttccatt accatcccaa ccatatgtta gaacagcatg tacatcacta
     1381 gcattcacgc ttttgtttgg tttgactgga atagtaatga cttgttgtgt catgtcataa
     1441 acaatccgat cagttttcac agtaaatgaa agattctcat ttgcccgttc atttgcagaa
     1501 gtactgattc caatcattgc tgcaattata ctcagcccaa gaataacaaa taatacggtc
     1561 gctgttttct tcatttctat cactctccat ataattacct tctatttgat aaaggaatta
     1621 tataaaatga aaatgatgag aacacgacag tcagttgtga aaatctaaca aaagatgtcg
     1681 tgtattcgct tgaatttgtt tactttttcg tttaatcaat gacagactta aaaaagcaag
     1741 accgatatca gccttgcttt cttcatcttt tattgataaa cagcagtgta tttcttcatc
     1801 cgctttgttt tatatgcata gagtaacaaa ccaacaatca agactaacaa gaataatgta
     1861 gttgatatag atgcttcagc cccaaagttt cccccagtaa ttattgtatt ttgcgattgg
     1921 atcagagtgg agaaaaaacc ttgttcttgc gttccgctga ctggtatacc caaaatagct
     1981 ccttgtgtcc aattccaagt aatatgagca ccaatagccg taaataaact tcctgtactg
     2041 tagataagtg cgctgaaaaa gcaacctgct aaaaagatat tgaaaactgc cagaaatgtc
     2101 atgttaggat tcaatccgtg caaaagagca aataatgcag cagatccaat cattgctatt
     2161 aacggaccaa atctaagcat ctgatgctga aaaaagccac ggaataaaag ctcttctgat
     2221 gcccccactg caataaataa aataaaactc gttatcagcg gcacaatgtt tgcgtttcct
     2281 gaatctaacc gatacgcccc cgtcaaatat aatggactag taataagcag aaacactcca
     2341 attccaacaa cgagacctcc cgctaaatac ccccagtctt ttcctcgaac agaaacacca
     2401 taatcttcta ttctttttcc ttcgacctta ctagcaagaa ataaagctag taaaaaaaca
     2461 gctaaatgtc cgaccgtacc ggcaattgct ccaatccatt cactatttcc aataaacata
     2521 tccgcaagtg cattattgac aagaattcct aaaacaaaac caactaagac tattaaaaca
     2581 gatccaatca accgaataat cctgatcatc tttcatccct cctttatatt aagtttaaca
     2641 acccttttct ttcttgtctc gtgaaaacta tgtaaatgtt ctccatcata gagagaatag
     2701 atttttacga actaagaata tctacagcaa ccttacgaaa atgaaagact gtctccctaa
     2761 ttcttctgtt agactgtttt taataaggaa aggatgggaa attttttgaa aaaacaacta
     2821 ctaattatgt gcctttcagc atgtgcaatt ttactattct caggttgtag caagtccaat
     2881 gaaacgattg caacgtttaa aggtggaaca cttacagttc aggattttta tgataaccca
     2941 aaagttaaat caaataatca aacactatta aaaaaaatga ttgtcttcaa agcatttgat
     3001 gatgtatacg gaaaagagat ttcttcaaag gaagtaacaa aagaatataa tgaacaaatc
     3061 aaaaaactcg gtccaaacta caaggaacaa ttaaaagcag ttggccaaac agaagagaca
     3121 tataagaaac tctttaaaca gatgctggct ttccaatacg gtttgaaagc caatgtaaaa
     3181 ttaacagata aagatctaga cactgcttgg aaagaattct atccagaagt tagtacacag
     3241 atcattctat tttcgactaa agaagaagca gataaagcaa aaaaagaagc aaatgaaggg
     3301 gaaaatttct ccaagcttgt tcaagcttat ggaaaaaata aaacattaaa ggaaactgat
     3361 ggaaaaatga attttgattc aaccaatcca gagattccta ctgaagtaaa aaaagcagca
     3421 tttaaactaa aaaacgggga agtttctgat atcattcctg taacagatcc aacgacttat
     3481 cagcaaagct attatctagt aaaaatggta aaaaaacagg ataaaggttc taataaagac
     3541 aaatacaaat ctgagctaga aaaaattgct accgaagcaa aaactcttga tacggaattc
     3601 atggataaaa caatccgtaa agtgatgaaa aaagacaatg taactattaa agatccttat
     3661 gtaaaaaata tattcaaata aacaaatttg acccaattgg ggatgtggca aaatttgaga
     3721 agtgattttt ggaaattcct tcttattttt gaggcaaacc acttttgcca caacttcatc
     3781 ggggcctttt tttatttgta ccaaaacaaa tcactggatt tgttacaatc atgaggatat
     3841 aatttaaaaa agttttttat ttctatcaca tgaatcaatg cgccattaag tagattcttt
     3901 catgcttccc tattttttca cttgccgtat cgcttcaaaa acaactatca taaactcaaa
     3961 ctcgactgaa aagttatttt aaaaaagact aaaaatatcc cttctggact tgttcctctt
     4021 attctgttat actagagaaa cacgaaagat atttttatgc gacacttttc atttgttttt
     4081 atgaaattta ttcaaaactg gcgtaacttc agagttacaa ggatgtatta gaggttaggg
     4141 aatacatcgt ttaaatgaga aataagtaat agtcacccaa actttaaatt aatgaatatt
     4201 gaatcgtcag atagcctcct cgagttttcg agaaggctat cgtttatttt atggagaaat
     4261 tgattcagaa gtaatcagcg acctttttta gttcaccaat cgtttctgct tggaaagttt
     4321 gcacccaatc ttcttggaag cccctcactg ctttatcaat cgagggcatc tctaatactt
     4381 gatctagtag ctctttacct aacgttatcg catgagctcc agatcgaatg gctgtagtga
     4441 cttgttcaca atttttgaaa ctagctgcta atatttttgt tggtctttgc gtacgttcga
     4501 tctcagtccg caacgaatca atcactttca tagcatcaat gccttgattt tgcatcctgt
     4561 tataataagg tgcaatataa tctgctccat tattaatagc taaataccct tgtaattctg
     4621 tgtaaatagc tgtaccggta atcaaataac cttctttttt caactttttg atcgcttgaa
     4681 ggcctgcttg attcaccggc accttgataa acgtatcttt tcccagttct tctatgataa
     4741 ctcgagcatc ctctatcatt tcttcttccg ttcttcctat tgtctgaata tggataggaa
     4801 tctgtccgat tatttttttg atttctttca tatgcgagaa gaagtttgac gctccttctt
     4861 ttttcagaat tgtaggattt gttgttacgc ctgttaaatt caaaaactgt tgacaagaat
     4921 aaatactttt tgtatttgct gtatcaagca tgatttccat atagctttcc cccattctga
     4981 tcctcttgat cgattttaat gattgtcccc tttcttaaat gcggcatttc cttatcttct
     5041 acatacattg ttcctggaag ttccggctca tgagatccat caaattttat cgtgatatgt
     5101 cctaattcat tcaaattagt ttggacaaca tctcctactg ctgtgattac atacatttgt
     5161 ttatcaaaac agagcttcat atcacaagtt atttcttttc ttacctgatt caattcaatc
     5221 ttataacaat aatctgttaa tgtttccggt gcttcatctc caaataaaat cattattttt
     5281 tctttcctga atacttctgc ttctatacca acatctgtta caaccgtttc aaagatcgtc
     5341 atc
//
LOCUS       DAZKAS010000156         5259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000156 DAZKAS010000000
VERSION     DAZKAS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.160"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..352)
                     /locus_tag="ACG8H7_002479"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="ACG8H7_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="ACG8H7_002480"
     CDS             complement(612..1160)
                     /locus_tag="ACG8H7_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984540.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="ACG8H7_002481"
     CDS             complement(1161..2018)
                     /locus_tag="ACG8H7_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP8984541.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2591)
                     /locus_tag="ACG8H7_002482"
     CDS             complement(2304..2591)
                     /locus_tag="ACG8H7_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP8984542.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2581..2910)
                     /locus_tag="ACG8H7_002483"
     CDS             complement(2581..2910)
                     /locus_tag="ACG8H7_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP8984543.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3052)
                     /locus_tag="ACG8H7_002484"
                     /pseudo
     CDS             complement(2987..3052)
                     /locus_tag="ACG8H7_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            3088..3657
                     /locus_tag="ACG8H7_002485"
                     /pseudo
     CDS             3088..3657
                     /locus_tag="ACG8H7_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            3792..4344
                     /locus_tag="ACG8H7_002486"
                     /pseudo
     CDS             3792..4344
                     /locus_tag="ACG8H7_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015474649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            4496..4729
                     /locus_tag="ACG8H7_002487"
     CDS             4496..4729
                     /locus_tag="ACG8H7_002487"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8984544.1"
                     /translation="MFQSFIHEKQRELSIKMIQALKTFRQSEEAIVNALNYHYSNGLV
                     EGINNKIKVIKRTAYGYRNFSNFRNRIFIEREL"
     gene            4814..>5259
                     /locus_tag="ACG8H7_002488"
     CDS             4814..>5259
                     /locus_tag="ACG8H7_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFP8984545.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDSDTLPMKGKRKPNGHQEKRGKQQYQRSIHDR"
BASE COUNT         1651 a          996 c          841 g         1771 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccagtccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgccc ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataaggaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag cttttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttatttgata
     3061 gtagatttaa aactttgcaa cagaaccatt gtagctattg gggcaaagac ttattcagct
     3121 cactcgattg ccggcagcat tgaaagtttt gtttatatgc cagcttatgg attagctacc
     3181 gctgcagcaa ctttagcagg aaatagtatt ggaaaaaaag actatgtgga aacaaaacgt
     3241 gttgcatttt atgcggttaa atacggcgtg attgttttaa gtatcctagg tataggattg
     3301 ttttttggta cgccatatat tgcaccttgg tttaccacag atgctgaggc aataaagcaa
     3361 attgttattg cgctaagaat tgacgctttt aatcaaccag gactagctat cagtctaata
     3421 ttagctggcg tactccaagg aatgggagat actaaaaccc cattatacag tactgcattt
     3481 ggaatgtggg tcacacgcat attaggtgta atacttttag ggaatatatt ggatttaggg
     3541 attgctggag tgtggttagc aattggcatg gatctatata tacgttcact ttttttaatc
     3601 tatcgtttca gacgaaatat ccaaatttta aataacaata acgaaacacc ttcctaaacc
     3661 agtgattttt tatcgctggt ttttgccatt tagcgattaa aaaaattgcc atataaatag
     3721 aaaagtaaca caaggggtac aatggtgtca taactaatgg tcataagtga agtgaaataa
     3781 ggggtgaaaa catgaaatat ggctatgcac gggtgagtac ccgccaccaa gatctggaag
     3841 ggcaaatgcg tcagctggaa gaggaacgct gtgataaaat tttctttgaa aaaataacgg
     3901 gaacgaagag tgatcgtcca gaattccaaa aactgcttca ggaagttcag actggtgata
     3961 cgttagtggt cacaaaatta gaccgatttg ctcgtagcac gcaggatgct cttcatacaa
     4021 tcaagcttct ttttgaaaaa ggcgtgcgaa ttaatgtttt gaatttgggc attattgaga
     4081 acacgtctac tgggcgatta attattcact atttttagtg cctttgcgga ctttgaacgc
     4141 gatttaattg tggaacggac acaagaggga aaagaaattg ccaagcagcg gcccggtttt
     4201 agagaagggc gaccaaaaaa attttccaaa caacagatga ctttagcaat gaaactacta
     4261 gagacaaatt cctataaaga agtagagaaa atgactggta ttagcaaaag tactttaacg
     4321 cgttataaaa ggaagaagag ttaggagcca cagaataccg atatcatcgt cttttcaaag
     4381 gcatggtgac ggaaagggga atcgttgatt attttctttc cttggacgaa ggtttacgcc
     4441 gccattacaa cgtctaccaa acaactgtct ttgccattaa ctatcggaac cgaaattgtt
     4501 tcagtccttc atccatgaaa agcaacggga gctttcaatt aagatgattc aagccttaaa
     4561 aactttccga caatcagagg aagccattgt caatgcgtta aattatcact actccaatgg
     4621 tttagtggaa ggtatcaata ataaaatcaa agttattaaa cggacagcct atggctaccg
     4681 caacttttcc aattttcgta atcgtatctt tattgaacgc gaattgtaaa attaagttag
     4741 aaaaataaaa aggcatttat ggtacactca aattgtattt ccgacgaaag aaaacaaagg
     4801 agtgtaacca taaatgacct acaaacatct taccatagac gaactgacaa tgatagaatc
     4861 atattatctt caacataata agccggttga aatcgctaac cgaatgggac gtgctataca
     4921 aactatttat aatgtagtca ataagttcaa gcaaggtaag actgctcttg attattggca
     4981 ccagtataaa gaaaataaga aaaaatgtgg tagaaaagtc attcaattac ctgctcatga
     5041 agtagattac attaaagaga aagtcactct tggttggacg cctgacgtca ttatcgggcg
     5101 aaaagaaaga cctgtttcat gcggtatgag aacactttat cgtttatttt ctaaaggaat
     5161 atttgatagt gacacactac cgatgaaagg taaaagaaaa cccaatggcc atcaggaaaa
     5221 acggggaaaa caacagtatc agcgctcaat ccatgatcg
//
LOCUS       DAZKAS010000157         5180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000157 DAZKAS010000000
VERSION     DAZKAS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.161"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..790)
                     /locus_tag="ACG8H7_002489"
     CDS             complement(62..790)
                     /locus_tag="ACG8H7_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFP8984546.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="ACG8H7_002490"
     CDS             complement(803..2083)
                     /locus_tag="ACG8H7_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFP8984547.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="ACG8H7_002491"
     CDS             complement(2224..2826)
                     /locus_tag="ACG8H7_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFP8984548.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="ACG8H7_002492"
     CDS             3071..3277
                     /locus_tag="ACG8H7_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984549.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="ACG8H7_002493"
     CDS             3589..4308
                     /locus_tag="ACG8H7_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFP8984550.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="ACG8H7_002494"
     CDS             complement(4389..5072)
                     /locus_tag="ACG8H7_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFP8984551.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
BASE COUNT         1791 a          880 c          948 g         1561 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc
//
LOCUS       DAZKAS010000158         5044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000158 DAZKAS010000000
VERSION     DAZKAS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.162"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..254
                     /locus_tag="ACG8H7_002495"
     CDS             <1..254
                     /locus_tag="ACG8H7_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8984552.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(424..1386)
                     /locus_tag="ACG8H7_002496"
     CDS             complement(424..1386)
                     /locus_tag="ACG8H7_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8984553.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1388..2827)
                     /locus_tag="ACG8H7_002497"
     CDS             complement(1388..2827)
                     /locus_tag="ACG8H7_002497"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP8984554.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3012..4958
                     /locus_tag="ACG8H7_002498"
     CDS             3012..4958
                     /locus_tag="ACG8H7_002498"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP8984555.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1628 a          854 c          830 g         1732 t
ORIGIN      
        1 gaaacaaatt gaaggagtac aaaatctttc aaagcatgtc tcggaaaggg aactgcttag
       61 ataattcact tatggagaat ttttttggct tattaaaaca agagattttt cacgggaaag
      121 tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat tactacaata
      181 atgaacggat aaaacagaaa ttaaactggc aaagtcccgt ccaatttcgt aaaacaaccg
      241 taacagttgt gtaaaaatat cattttagag catgaaaaaa ggagtggatt tctccactcc
      301 aaaaaagtct aacttttggg ggtcactaca agttgacaac tcctttttgt ttacaaatca
      361 attggatatc tacaaattgt attttctaac gaattttata gacattaaaa aattaattta
      421 acgttaaata cttcctccag aaagtattga gattggtaaa atataatcct tatttgtctt
      481 ttcgccttta atttttttca gaagaatatc aacaataaga cttgcaattt catcaattgg
      541 ttgtcttatt gttgtcaatt gtgggaaaaa tttctcaata aaagaggtac catcgtatcc
      601 aataattttt aaatcttctg ggatattaag tttaagttga tgagcaattt tcatagtgag
      661 aatggctgtt aaatcatcag aaacaaaaat accatctggt ttgtgattta taatagttga
      721 ctttatttcc atttcacgac ggatagtaga cagattatta ggaattttga aaatttttcc
      781 atttggattt tgaaaagaaa aacctagtgc acgtaattct gtgggagaat ccgtgttatc
      841 atttcctgta atcataatgg tattttgaca accatttttt tgtaaagtct tagctgcaag
      901 ttttccccct tcaaagttat ctgaagaaac aatggaaaca ttaggagcta aatttctatc
      961 aaaagcaaca ataggtgctc tcacacgctc ataatcagca attccaagat tatggcttga
     1021 tgagattatg ccatcaactt ggttggcttc aagcatttca atgtattctc tctctttagc
     1081 aggatcattt tgactattgc aaataatcgt tttatatcca tgtttaaata actctatttc
     1141 gagatactca atgagttcag agtaaaagat attactaata ttaggaaaaa taagaccaat
     1201 aagctgaggt gactttcctt gaagtccacg tgctaaatta tttggtttat aaccaagagt
     1261 tttcatagct tcatgaactt tttgttttgt tttttctgat aaatatccct tattattaat
     1321 gacgcgtgaa acggttgttg ggctaactcc agctaattca gcgacatcag ttaattttgc
     1381 taccatttta atattttaat tcaaagtatt ttccagtagg ttcgcctgat ttgatttgaa
     1441 taccattttg gtgattttca gggaatacac gactagtaaa cactttttct cctttattaa
     1501 taaaaatctc aatgattgag ttatctacaa aaatgttagc agttgtttct ttagtatcaa
     1561 ttgagcattc acgaatcgta ccaaagtcag tggcatattg aatgcctgct ttgctgcgat
     1621 ctaaaataat ttttccttct ttggtatcta caatcaagct aagtccattt cctttactat
     1681 cagagaaaag aagtaactcc gatttctgat ttggaggaaa agttagttca agctcataag
     1741 tattatttgt ttctgctttt gctgtaaatt gttcagaact agcacgtaat gatgtaatag
     1801 cttctactgg atattggtaa agtttaccat cttttattga aagttctttt acaagactca
     1861 ttgctccctg ataatcatac ttatcagttg gataatcgat atcaggtagt ccaatccaac
     1921 tgacaatcag agcacgacca tcaggagtat tgaatccttg agtcgcatac gcttcaaatc
     1981 cataatcaag attttgaatt tctgaagcgc ccacaagttt tactttttct atgtcaagcg
     2041 attgacagat cttaaaagta ttcggataga tattcccata atttagctca gatttatcca
     2101 acccttgagg acaataaatg agaacaggtt ttttgtctac aaatactaga tttggacact
     2161 caatcatgta ttctgaacca gtgccgccaa aatcaaggtt gccaacttcc tcccatgttt
     2221 ccacgttatt gtcatccgct ttgtaaagtt tgataaagcc tgatttttct aaattttgtg
     2281 ctccaacaat cgcatacaat tgtccttcat aattaaacat ttgtggatca cgaaaatgat
     2341 cagttacatc agcaggttta tgaataagaa cattctcacc tttcgtaatg ttgtagtctt
     2401 tatccatcca tgctccgatt tgaagtgtat cacgttccca attttcattt cgaacgtttc
     2461 ctgtatagag taagaataga ttcccctcta tttcatatgc agaaccggaa taagcgccgt
     2521 ggctatctcg ttttgtgtct ggaagaagtt ctgctcctgt tacatgaaaa tgaactaaat
     2581 catctgattc attgtgtacc cattctttca acccatgagc agctccaaat ggccaatttt
     2641 gataaaaaag cgtatatttt ccgttaaaaa aagaaaaacc attaggatcg tttaacaatc
     2701 ctgttttagg ttcgatatga tagctagcac gccaaggtga tcgttttaca ttatccttaa
     2761 ttttatccat ctcatcttta gtccaatcag aataagcacg atatcgtatt tctcgaggta
     2821 agttcatatt ttactccttc aatttatctt tttacaacta gtatggtaaa cgttttccgt
     2881 tgaaaaatca atcatttcat ttaaaaaaat aaactatatg acaaacgttt gacatatttt
     2941 taagatatgc taaaataaat ttgcatttca gtgaaccgtt ttcacgtaaa aataaaaaag
     3001 gagtgattcg aatgaattac agtaaagtag cttccgacgt aatacaagcg gtcggaaaag
     3061 ataaccttat tgcagctgca cactgcgcta cacgtcttcg tctcgtacta aaagatgaca
     3121 cgaaagtgaa tcaaaaagct ttagacgaaa atccagatgt gaaaagcaca tttaaaatta
     3181 atggacaata tcaagtgatt atcggtgcag gtgatgttaa tttcgtttat gacgaactta
     3241 tcaaaaaaac tggactttcg gaaatttcaa cagatgattt aaaacaaatt gctagtaaag
     3301 ataaaaaatt caaccccatt atggcattaa ttaaattatt atcagatatt tttgttccaa
     3361 ttatacctgc ccttgttgca ggtggtttat taatggcatt acaaaacttt ttaacagctg
     3421 ctggactttt tggaccaaaa tcaattgaag aaatgtatcc tgcgattgaa ggtatttcct
     3481 caatgatcca actgatgtcc gctgcaccat tcatgttctt accaatttta gtaggtattt
     3541 cagctgctaa acgttttgga gcaaaccaat ttcttggtgc tgcaattggt atgatcatga
     3601 caacaccaga tcttggtggc tcaactgaat attggaatat ttttggttat catgtttcac
     3661 aaacaaacta tgcttatcaa gttattccag ttttagcagc tgtttggata ttatctatat
     3721 tagaaaaatt ctttcataaa aaattgccat catctattga ctttacattc acgccattat
     3781 tatcagtaat gattactgga tttttgactt tcacagttat tggccctgta atgttattgt
     3841 tgtctaatgg aattacagac attattgttt ggctatataa cactactgga tttattggaa
     3901 tgggtatttt tggaggaaca tattcactta tagtcatgac tggtttacat caatcatttc
     3961 cagctattga aactcaatta ctttcagcgt ggacaaatgg tatcggacat ggagacttta
     4021 tctttgtagt tgcatctatg gcaaacgttg ctcaaggtgc tgcaacattt gccatttggt
     4081 tcttaactaa aaattctaag acaaaaagtc ttgcttcctc tgcgggtgtc tcagcacttc
     4141 ttggaattac agagcctgcc ttgttcggag ttaacttaaa ataccgcttt ccattctttt
     4201 gtgctttaat tggttcagga attgctacag cagtagcagg attacttaaa gtagttgctg
     4261 tttcacttgg ttcagcaggt ttcttgggat ttctttcaat taatgcaaca tctattccat
     4321 tttacgtatt gtgtgaattg attagctttg tagttgcttt tgcaattact tacttctacg
     4381 gtaaaacaaa agctgctggt atctttaaag cagaagaaat tgctgaacaa acaccagtta
     4441 attcttcaaa agtagcaaca acagatccaa tatctactga tgttgaatca acacaaacaa
     4501 aagtttcatc agaaacaatt gttagtccca tttctggtaa agtgattagt ctttcttcag
     4561 taaatgatcc agtcttttca tcagaagcta tgggaaaagg gattgccatt aaaccaaatg
     4621 gaaacacggt ccattctcca gtagatggta ctattcaact cacgtttgaa actggacatg
     4681 cttatggtat taaatcaaat aatggtgctg aagttctaat tcatattggt attgatactg
     4741 tatcaatggg aggaaaagga tttacacaaa aagttactac taatcaaaaa gttagaaaag
     4801 gcgatattct tggagcattt gaccgttcaa tgattgagaa agaaggtctt gatgatacaa
     4861 caatgattat tgtgaccaat acttcggatt attcagaagt aaatatagta gctgaaaata
     4921 cagtcactga gggtactgat ttacttgaaa tcaaataaat atatttccct gagtaaagat
     4981 ggaattttct ttccaaaatc aaataatata gattaatcaa atatttgaga ataacatttt
     5041 attt
//
LOCUS       DAZKAS010000159         4996 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000159 DAZKAS010000000
VERSION     DAZKAS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.163"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(200..1183)
                     /locus_tag="ACG8H7_002499"
     CDS             complement(200..1183)
                     /locus_tag="ACG8H7_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFP8984556.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1188..1835)
                     /locus_tag="ACG8H7_002500"
     CDS             complement(1188..1835)
                     /locus_tag="ACG8H7_002500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFP8984557.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1920..2552)
                     /locus_tag="ACG8H7_002501"
     CDS             complement(1920..2552)
                     /locus_tag="ACG8H7_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP8984558.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2568..3119)
                     /gene="coaC"
                     /locus_tag="ACG8H7_002502"
     CDS             complement(2568..3119)
                     /gene="coaC"
                     /locus_tag="ACG8H7_002502"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFP8984559.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3116..3853)
                     /locus_tag="ACG8H7_002503"
     CDS             complement(3116..3853)
                     /locus_tag="ACG8H7_002503"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFP8984560.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3850..4839)
                     /locus_tag="ACG8H7_002504"
     CDS             complement(3850..4839)
                     /locus_tag="ACG8H7_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP8984561.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         1445 a         1025 c          971 g         1555 t
ORIGIN      
        1 ttcggaaaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
       61 ataaacggcg gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga
      121 aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc
      181 ttttttgatc agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt
      241 ttgcttccat atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga
      301 ttggccgaat cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga
      361 gctctgcttt atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt
      421 tcccaatcaa tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca
      481 agacccaacg tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga
      541 tgaaatccaa aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa
      601 agtcttgttt tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt
      661 tttcttctga tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga
      721 tcgctgctgt tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta
      781 attttccaag ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag
      841 cagaaacata gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat
      901 attcggcata accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg
      961 agatatcact tttattctca actacctctc cagctacttc gactcccaaa atagggtagg
     1021 gaggtttcaa agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat
     1081 ggactttgat cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta
     1141 atacatctac agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt
     1201 gcccattctt ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta
     1261 cgagaaatat ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg
     1321 atcgtttttg atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg
     1381 ttagtcaatg tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca
     1441 tctgcataat gtttagtaat ataataatct gtcatggact caggccattt tgctggttca
     1501 tctgcataaa cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc
     1561 actgttttga ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca
     1621 gcaaaaatca atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt
     1681 aaatcaaaag agacatacga aacattctct ttttttacta atgtttggga agcaacatct
     1741 ctcacccctg caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca
     1801 atttgtccgt ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat
     1861 ctttccattc aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat
     1921 cagttagtaa gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc
     1981 gcaagacctg cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct
     2041 gaataaacag catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag
     2101 tagatcatat tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat
     2161 aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa
     2221 ggaaccaccc caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca
     2281 tgttctgttc cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata
     2341 actgtgctac ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt
     2401 ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga
     2461 ccgataggga taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc
     2521 gcagttaatg taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt
     2581 tcattaagag tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt
     2641 ccaaaatctc cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat
     2701 ctcaatgttt ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca
     2761 ggggcaatca attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg
     2821 tctgcaatgc cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac
     2881 aagtcagctt gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg
     2941 acgtctgtat ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt
     3001 gtgctgcttt tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta
     3061 agatcagcgc ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt
     3121 ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag
     3181 cttcttccac tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt
     3241 ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga
     3301 ctgccagaag agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt
     3361 ttggctgcca ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa
     3421 gatgctcggt gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa
     3481 accaattgtc cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt
     3541 ctgaaaaact aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt
     3601 agtgttcttt tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg
     3661 tataaatcgt tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat
     3721 cgattgtggt atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg
     3781 tcgaatgatt tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga
     3841 ttagaatctt catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc
     3901 aagtagttta gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc
     3961 atctgcaatc tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac
     4021 attgacagaa tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc
     4081 gtttagtttg attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa
     4141 tggaagaaga ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg
     4201 ttttcctaaa gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc
     4261 acctagactt tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg
     4321 gaatcctagt ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt
     4381 aacgacaact tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat
     4441 agcagtaaac cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag
     4501 aggaactaac cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac
     4561 gccaagccca aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact
     4621 ttggacgaca gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg
     4681 accagactcg ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac
     4741 caagacttca ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac
     4801 aggttccttt attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa
     4861 attttctttt atcataccat aaagcctact cttttattct tgttcttccg taaatttaga
     4921 caaaaattag gtgaaaaata aagaatcaaa caactagaat ttattccaaa agataaagaa
     4981 atattcagca aaaaaa
//
LOCUS       DAZKAS010000160         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000160 DAZKAS010000000
VERSION     DAZKAS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.164"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..854
                     /locus_tag="ACG8H7_002505"
     CDS             42..854
                     /locus_tag="ACG8H7_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFP8984562.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            851..1651
                     /locus_tag="ACG8H7_002506"
     CDS             851..1651
                     /locus_tag="ACG8H7_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP8984563.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1812..3080
                     /gene="rseP"
                     /locus_tag="ACG8H7_002507"
     CDS             1812..3080
                     /gene="rseP"
                     /locus_tag="ACG8H7_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFP8984564.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3148..4857
                     /locus_tag="ACG8H7_002508"
     CDS             3148..4857
                     /locus_tag="ACG8H7_002508"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP8984565.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
BASE COUNT         1604 a          816 c         1103 g         1410 t
ORIGIN      
        1 cttaagatag tgggaatctt ttaagtatag gaggaaaaag aatgttacgt ttttttccgc
       61 aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt taataccgat ggaccaattc
      121 ctcagcacat tgccatcatc atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc
      181 cacgagttgc tggccataaa gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat
      241 caaggcttgg agtgaaagtg ttgacgcttt atgccttttc gactgaaaac tggaagcgac
      301 caaaagatga agtaagtttt ctcatgcaac tacctgtcga cttctttgat acgttcgtac
      361 cagaattgat taaagaaaat gtcaaagttc atgtcatggg ttatgaaaac gtattgccag
      421 aacatacgca agatgctgta agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg
      481 tattgaattt tgctttgaat tatggaagca gagcagagat cgtgactgct gtccaagaaa
      541 ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga aatcgatgat gaattgattg
      601 ctaagcattt aatgacaggt ttcttgccta aagagttaca agatcctgag ctgatgatcc
      661 gtacaagtgg agaagagcga attagtaatt ttctgttatg gcagatcgca tatagtgagc
      721 tgtattttac aaaagcgctt tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg
      781 cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag
      841 gggatcagtc atgaaacaac gtgtggtcac agcagccgtg gcgctagtct tgtttattcc
      901 gattatatgg atcggaggca tagcggttga gcttacagca gcaatcttag cagtaatagg
      961 agtttacgag ctgtttcgga tgaaaggatt gacactctta agttttgaag gagttttatc
     1021 cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga tggtttttct ttcttccaga
     1081 gaatgtaagt acatttatgt tattttatct tatagttatg attcttttag gaacatcagt
     1141 tatttctaaa aatacgtata cgattgatga agcaggtttt cctgtgcttg tcagcttgta
     1201 tgtgggaata ggtttccaga attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat
     1261 atttggtcta tttattgttt gggcaactga tatcggagca tatatggttg gtcgacgtta
     1321 tggtcagcat aagttaagtc ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg
     1381 tggtattggt agtgctgttg tagtcgcatt tctttattta ttgctatacc cagcaaaaga
     1441 actatttaat catggtatgt ttgtcatgct tctgatgacg attatttttt cagcagtcgg
     1501 acaatttggt gatttggtcg aatctgcaat caaacgtcat tatgatgtga aagactcggg
     1561 aaatatctta ccaggccatg gtggaatctt agaccgtttc gacagtttgc tgtttgtatt
     1621 tcctatcatg catttatttg gcattattta gtaagtatac ctgcgtttct ttgtgaattt
     1681 cataaaagac gcaggttttt atttttcatt atattgatac tattggtgtc tttggtatac
     1741 tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg
     1801 agaaaaaagc tatgaaaacg attctgacat ttatcatcgt ttttggtata ttagtgattg
     1861 ttcatgagtt tggtcatttc ttctttgcga aacgatcagg aatcctcgtt cgcgaatttg
     1921 ctatcggtat gggtccgaaa atttatgggc atcaagcaaa agacgggaca acatatacgc
     1981 ttcgcctatt acctattggc ggttatgttc gaatggcagg caatggggat gatgaaacag
     2041 aaatggcacc aggaatgcct ctttctcttt tactaaattc tgacggcatc gtagagaaga
     2101 taaatttaag taagaaaatc caattgacaa atgctatccc gatggaattg agccgttatg
     2161 atttagaaga tgagctgact atcactggtt atgtgaatgg agacgaaaca gaagtagtaa
     2221 cctaccctgt tgatcacgat gcgacgatta ttgaaaacga cggaacagaa atcagaattg
     2281 cgccaaaaga cgtacagttc caatcggcta aattgtggca gcgtatgctg accaactttg
     2341 ctggacccat gaataacttt attttggcaa tcctgttatt catcattctg gcatttatgc
     2401 aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga gatcatgcct aatggagctg
     2461 cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc
     2521 attcatggaa tgatctgaca acagtcatta cgaaaaatcc ggataaaaca ttagacttta
     2581 agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa
     2641 atggcgaaaa agttggccaa cttggtataa aagccccaat gaatacaggt tttatggata
     2701 agatcattgg agggacgcgt caagctttca gcggttcatt ggaaattttc aaagccttag
     2761 gatcactatt tacaggtttc agtttagata aattaggcgg acctgtcatg atgtaccaat
     2821 tgtcttcaga agcagcaaat caaggtgtaa caactgttat tagtttgatg gcgcttcttt
     2881 caatgaatct cggaatcgtc aatctgcttc cgattcctgc cttagatggc gggaaattag
     2941 tcttgaatat ttttgaaggg attcgcggga aaccattaag tcaagaaaaa gaaggaatat
     3001 tgacattagc tggctttgga tttttaatgt tgttgatggt gctagttaca tggaacgata
     3061 ttcaacgatt ctttttctag aaaaagttga caaatagggt ataataacaa acgataacta
     3121 ttttgaggaa ataaaggagt atatagaatg agacaatcaa aaatgttgat tccaacatta
     3181 cgcgaagttc caaatgatgc agaagtatta agccatcaaa tcttgctaag agctggatat
     3241 atccgccaag tatcagcggg tatttattca tatctgccat tagcaaatcg tgtattggaa
     3301 aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg
     3361 cctgccatct tgccagcaga actttgggaa gaatccgggc gttacgaaac atatggacca
     3421 aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga tccttggacc aactcacgaa
     3481 gaaactttta ctgatttgat gcgtaatgaa atcaattcat ataagaaatt acctttaaat
     3541 ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc
     3601 ggaagagagt ttatcatgaa agatgcgtat tctttccatg caacggaaga aagtttggac
     3661 gagacatata aagattacga aaaagcctac accgaagtat tcaagcgttg tggcttagaa
     3721 tttcgctcga ttatcggaga cggtggcgca atgggcggga aagactcaaa agaattcatg
     3781 gcgatctcag aaatcgggga ggatacgatc tgttattcga ctgaaagtga ttatgctgca
     3841 aacttggaga tggctacaag ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa
     3901 ttggagaaag tagctacacc taacgtaaaa tcaattgacg aagtagcaga tttcttttct
     3961 gttgaacctc aaaaaatcat caagtctgta ttgttcattg cggatgaaca acctgtcctt
     4021 gttttggtac gcggagacca tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct
     4081 gactttttag aagaagcaac agaagaagaa gcacaaaaat atctaggtgc tgagtttggt
     4141 tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa
     4201 gatttagcga atgccattac tggagcgaac gaaacaggat tccactataa aaatgtgaat
     4261 ccagaacgcg atttcactgt actttcttat gaagatcttc gttttgtaca agaaggagat
     4321 ccttcaccag acaacaatgg tgttcttgcc tttactcgag gaatcgaaat cggacatatc
     4381 ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa ctgtactaga cgaaaatgga
     4441 cgagaaaaat ttgtgattat gggctgttat ggtatcggtg taagtcgttt attatcagca
     4501 atcgtagaac aacattcaga tgaacaagga atcaactggc caaaaggaat tgcgccattt
     4561 gatttgcata tcgtccaaat gaatctaaaa gacgaatacc aaacaaacct gacgaatgaa
     4621 ctagaagaag cgatgacaaa agctggctat gaagtactgg ttgatgacag aaatgaacgc
     4681 gcaggagtga aattcgcgga ttctgatttg attggctgtc caatcagaat cactgtcggt
     4741 aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga aaactggcga aatggttgaa
     4801 gtacgtaaag aagaattggt gaatacttta ccaatcctct tgaaccaagg cgaataataa
     4861 gcgttaaaac acagaaaata aaaatagctg aatcagctgt ttttgacaat ctgatgattc
     4921 gcttttaaaa ttg
//
LOCUS       DAZKAS010000161         4858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000161 DAZKAS010000000
VERSION     DAZKAS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.165"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..877
                     /locus_tag="ACG8H7_002509"
     CDS             161..877
                     /locus_tag="ACG8H7_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP8984566.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            896..2479
                     /locus_tag="ACG8H7_002510"
     CDS             896..2479
                     /locus_tag="ACG8H7_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFP8984567.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2608..3192
                     /locus_tag="ACG8H7_002511"
                     /pseudo
     CDS             2608..3192
                     /locus_tag="ACG8H7_002511"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            3138..3656
                     /locus_tag="ACG8H7_002512"
     CDS             3138..3656
                     /locus_tag="ACG8H7_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984568.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3679..4704
                     /locus_tag="ACG8H7_002513"
     CDS             3679..4704
                     /locus_tag="ACG8H7_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP8984569.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
BASE COUNT         1797 a          530 c          954 g         1577 t
ORIGIN      
        1 attatgtaat agaaattata attatgatta ttttaattat tataaatatc agttatatta
       61 agcagttgtg ttatagagga agaaacatta tttcaatgat taagagtcga gagcacaaac
      121 ggtagacgat gttattttta taaaaagata taatttatta atggtcgata attatgaaag
      181 gaaaaatatt cttaaaaaaa agagtatagg atgtcgtatg aaaatagtcg ctatagtacc
      241 aatgaagtta aataacaaaa gattgccaca aaaaaacacg aaagctttta ccaatggaaa
      301 accattgtgt tattatatac tttcaacact tttaaaagta aaacagattg ataaagtgta
      361 tgtctattgt agtaacccag atattaaaga atttatacca gaaggtgtaa catttttaaa
      421 gagatctagt tccttagatc aggatacaac gagtattaca caggtattag ccggtttcac
      481 aaaagaagta gaggctgatg tatatgtaat gacacacaca acagcaccat ttattagcga
      541 aaaatctatt gaaaaaggac tagatgccgt gctgacagga gaatatgatt ctgcatttgc
      601 tgcaaaaaaa ttgcaagatt tcttatggaa agacggtgaa ccatttaatt atgaattgga
      661 caatattcct agaacacagg atttgccgcc tttatacgaa gaaactagtg gattttatat
      721 ttataatcgc aatgtgatga ccgagttgca tagaagaatt gggaaaaaac cttgcatagt
      781 tgaagtaggt gagattgagt cagttgatat tgatgaagcc gaggatttta tgattgctga
      841 tgcgatttat aatcatctta ttttacaagg tagttaagac agggggaagg ttaggatgaa
      901 caatgtacag attttagatt gcacacttag agatgggggc tattgtaaca attgggaatt
      961 tggatttgat aatacaaaaa aaattatttc tggacttata gaatcaggaa ttgagattat
     1021 agaatgtggg tttctcacaa ataaaacagt atacaataaa aatgttacaa aatatacgac
     1081 cttagaagaa ttagcacaga taattcctga aaaaagagaa ggaaaattat ttgtagcaat
     1141 gatgaactat ggcgaatata atatagagga tcttccagat tatgatggtt catctattga
     1201 tggaattagg attgcgtttc ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga
     1261 gataaagaaa aaaggctact tagtttttgt tcaagctatg gtatctctca attatacaga
     1321 tgaagagttt ttagcagtaa taaaaaatgt gaatcagttt aaaccatacg ctttttatat
     1381 tgttgatagt tttggaatga tgaaaggaaa agatttaact cgtctttttt acatgattga
     1441 gcataatcta gaaaaagata tacagatagg ttttcactca cataataaca tgcagcttgc
     1501 atattctaat gcacaaaaac ttataaccgt acaaacaaat cgaagcctga ttattgattc
     1561 ttcggtgtac ggaatgggaa gaggagcagg taatcttaac acagaactat ttgtagaata
     1621 tttgaatgat aatgcaggaa aaaaatatca attaaaacca ttattatata taattgatga
     1681 aatactaaat agtttctatc agaagagtta ttggggatat tcattaccaa actatatttc
     1741 agcagttcat aatgctcatc ctaattatgc aagctatctg tcatataaga atactttaac
     1801 aattgaagca atgaatgaaa tattcgaaat gatggatgaa gaaaaaaagg tatcttatga
     1861 taaacaatat atagaagatt tatactacaa atatatggaa actgggaaaa tacaggagga
     1921 atatatggaa gaactgagaa aatttctctc agacaaaaaa attctactga ttgcaccagg
     1981 aaaaagttct gtagatgagg tagaaagaat aaaagagttt gttaccaaag aagaagtcgt
     2041 aataattagt gtaaattttg aatattctca tatagatata gattacgtat ttttaagtaa
     2101 tttaagacgt tttcatgaat taccgaaaga aagcagaact aaatgtattg ttacatcaaa
     2161 tatacaagca tcggatgtat atttacggac gagttataaa aagttgttat ctgaaaggga
     2221 agttgtaaga gacaatgcag gattaatggc aattagattt ttagccgata tgaatgtgga
     2281 tgaaatattc ttagctggat ttgatggata ttcacataat gaagatgaga attatggtga
     2341 gcaatccatg gaaattataa caaaatttgc attattagat gcaatgaacg tgcaaatgag
     2401 agatgaattg tcagaattag ctaaaaaagt aaaggtgaca tttttaacgg atccacgttg
     2461 ggttcgaatt gataagtagt agtaataatg tttttaaaat tttgataaag agatagtgag
     2521 agaataaaaa tgggtaaagt cttaaataaa aaagaagaaa aatatttaag ggacgatggg
     2581 gcgagatgaa actctaattt tgaactttta agaatcattt ctatattgtg tattgttgct
     2641 taccatgcta atttgcgttt aaattataat gcagacggaa ttatagtaca tggttcatta
     2701 actgctaacc agattttctt gaattatatt ggtatttggg gtcagctagg agttaattgt
     2761 tttatattta tttcggcata ttttttaagt gagaaaagaa acattcactt tgaaaatgtt
     2821 ttgggaattg tattagaatg tattttttat agtgtattag tttatggaat tctttgtgca
     2881 tgtggaatag aaacattttc attgaacagc tttgtaaaag aaatattatc atttgctcta
     2941 ggtagatatt ggtttattac atcctattta gcattgtata ttctaagtcc gtatttaaat
     3001 atcatgctta acaatctgga aaaacttcag ttgcaaaaaa taagtatgtt attgtttata
     3061 ggtgtatgct gttatcgtat gttatttaaa acatctttgt ttggggattg tgctgtttta
     3121 attgcactat atgtcatatg ttatacactg aaaaaaacga taataatatt tttgagagac
     3181 acagaatcat aggaacaatt gtatgtataa cagttatacc acttctcata tccataggaa
     3241 tacttttcaa atttatacca aatgcaatgg ttaaccagat ggttcatatt ggttctccat
     3301 tgatggttat agcaagtata tttttatttt atgtatttaa aaatttttca ttttacagta
     3361 agtatataaa taaacttgcg agaaccagtt taggggtata tattttacat gaaaatcatt
     3421 atttcattga tggtatgaag tctagaggct taatatggga tgggatattt ggattaggca
     3481 aaaggttttt agatagggat ttttggattt ggtttataat tgcagttgta gttgtgtgca
     3541 taatttgcat ggctatcgat gttgtaagag aaaaatttga aagacagatt tttaaaatta
     3601 agagtgtaaa gaacctgtgt aaacttatga atcatgttat aaatatatac ccataattaa
     3661 taaatgagga gaaaagaagt gaaagttata tcattcggag aattattatt gagacttgca
     3721 gcacccggtt attcaaagtt atttcaaaga gatagtttgg agactacatt ctgcggaggg
     3781 gaagctaatg tagcggtatc cttagcaata tttggatatc aatcagaatt tataacaaag
     3841 cttccggatt cagatgttgg atatgcggca gctaatgctt tgcgttattt tggagtaaat
     3901 gttgataata ttatatttgg agagggacgc atgggactgt attatttaga aaaaggagca
     3961 gctcagagac catctaaagt aatttatgac agaaaatttt ctgcaatttc gttagctgat
     4021 agtgatgaat ttaagtggaa ggagatccta aacgatgcaa attggtttca ttggacaggg
     4081 ataaatccag cattgtctga gaatctcttt gagacgtcct tagatgcatg tcaggctgcc
     4141 aaaaaaagag gtataacaat ttcttgtgat ttaaattatc gatccaagct ttggacatca
     4201 gaggaagcaa aagaaaagat ggaacagata ctaccattgg ttgatatttg tatttgtaat
     4261 gaagaagatg cagaaaaagt gctaggaatt aaagctgagc atacaaatat taatagtgga
     4321 gaattaaata aagatggcta tgtgcaggtg gctagggaga ttcacaagaa gtttggatgt
     4381 aggtatgtag ctacaactct gcgtagaagt tattcggcaa gtagaaatgg ttggagtgca
     4441 atgttatacg aagcagaaaa agataaagcg tatttttcca cagaatacaa catacaaatt
     4501 gttgatcgcg ttggtggtgg agacagcttt tctgctggta taatatatgc gttatgtgaa
     4561 aatatgtccg gacaaaaggc tattgaattt gcgactgcat ctagttgttt aaaacatact
     4621 atagaaggtg attttaatcg aactacaatt gaggatgtat ataatttaat gaatagcggt
     4681 ggaaatggtc gcgtgttgcg ttagtcaaca tataaagata aatcacagta atttttatgg
     4741 tgattatctg tcttagtagt gattagaata acgtgaaaat aaattgttga gcgtttatac
     4801 tgttttgaaa aataaattta acatattgaa aaatacaaat atatacgtat attataaa
//
LOCUS       DAZKAS010000162         4852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000162 DAZKAS010000000
VERSION     DAZKAS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.166"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            800..1711
                     /locus_tag="ACG8H7_002514"
     CDS             800..1711
                     /locus_tag="ACG8H7_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFP8984570.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2198..2464
                     /locus_tag="ACG8H7_002515"
     CDS             2198..2464
                     /locus_tag="ACG8H7_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984571.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2536..2877
                     /locus_tag="ACG8H7_002516"
     CDS             2536..2877
                     /locus_tag="ACG8H7_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984572.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2932..3351
                     /gene="ssb"
                     /locus_tag="ACG8H7_002517"
     CDS             2932..3351
                     /gene="ssb"
                     /locus_tag="ACG8H7_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP8984573.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3428..3724
                     /locus_tag="ACG8H7_002518"
     CDS             3428..3724
                     /locus_tag="ACG8H7_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984574.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3739..4170
                     /locus_tag="ACG8H7_002519"
     CDS             3739..4170
                     /locus_tag="ACG8H7_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFP8984575.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4475..4657
                     /locus_tag="ACG8H7_002520"
     CDS             4475..4657
                     /locus_tag="ACG8H7_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984576.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1783 a          747 c         1024 g         1298 t
ORIGIN      
        1 aaaattgaag caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa
       61 taaacgtatt ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta
      121 tttgagggtt atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc
      181 ggcgcttggt atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt
      241 ggaaaaaccg ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata
      301 acataccaag cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat
      361 aaaatttaaa agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc
      421 cccctaaccc cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac
      481 gccccagtgt tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa
      541 gtgtagagga aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac
      601 aagtgtagag gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa
      661 acaagtgtag aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa
      721 agcagttgca gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt
      781 aaattgaagg tgatgaaata tgaagtattt attgcatggc tattttattg attattctac
      841 tcgcaatagt agtactttag cgattctatg tttgcttcat gaattgagag tggttactag
      901 aaaacagcta tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa
      961 tgctttagtg agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat
     1021 gtattattat ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa
     1081 agttcctgaa tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat
     1141 ggcattggca aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta
     1201 tgaaacgaag gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat
     1261 ttttcgtttt cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac
     1321 gttgaaaaca cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg
     1381 aacacgtcct gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact
     1441 tcatgcgttt aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga
     1501 attgtttgag cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt
     1561 attagagaat gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa
     1621 tggcgctgtt caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc
     1681 aaaagtgaag tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg
     1741 tgccagtttg ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga
     1801 ctaacttgtt tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat
     1861 agcagtcatt cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc
     1921 acgctgcgcg cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc
     1981 gctatacgaa aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa
     2041 aatcaaacaa gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc
     2101 cccaaacccc cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc
     2161 aaataaaaca ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact
     2221 gaaagaacag gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga
     2281 aggagatttt gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt
     2341 agacccaatg aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc
     2401 gcaagatttc actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt
     2461 ataaaagtca gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa
     2521 agaaggaaaa agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt
     2581 agttggggct acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa
     2641 acagcatggt ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga
     2701 aaagttgttc caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt
     2761 attgtaaaaa aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt
     2821 tgggacacgt ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg
     2881 gatagtttcc cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat
     2941 tcagtgaatt tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc
     3001 aatgcgacag gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag
     3061 cgtgaagcag attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa
     3121 tatactggaa aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag
     3181 aatcagcaag gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta
     3241 gagagtaaag aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct
     3301 tcaattccag gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat
     3361 aattttgttc tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg
     3421 attttttatg aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt
     3481 tgattataca gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt
     3541 tcgcttggta gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga
     3601 ttatgtcgga gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac
     3661 aggagaagaa cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga
     3721 ttaaagagga gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg
     3781 attgattttg ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa
     3841 ataaaacaag caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata
     3901 gtgaatcaaa ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg
     3961 caattttttt cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat
     4021 aaaagcagga tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa
     4081 caaaagaaaa tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa
     4141 cgtgcgacaa gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta
     4201 atcacaacta acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg
     4261 tctgatactc ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag
     4321 gcggcagaac aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa
     4381 attactcagg gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat
     4441 aaccgactta tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa
     4501 gaatgttggt catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa
     4561 aacatgtttg attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat
     4621 agagcaacta gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa
     4681 atcctctaca gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa
     4741 tttttaccat aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa
     4801 aaaatggaag cggttactta ttcttttgaa tctaagataa aaattcaaaa ga
//
LOCUS       DAZKAS010000163         4788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000163 DAZKAS010000000
VERSION     DAZKAS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.167"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACG8H7_002521"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACG8H7_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFP8984577.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACG8H7_002522"
     CDS             1019..1183
                     /locus_tag="ACG8H7_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984578.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACG8H7_002523"
     CDS             2141..3118
                     /locus_tag="ACG8H7_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP8984579.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACG8H7_002524"
     CDS             3128..3625
                     /locus_tag="ACG8H7_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP8984580.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACG8H7_002525"
     CDS             4001..4282
                     /locus_tag="ACG8H7_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984581.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACG8H7_002526"
     CDS             4284..4700
                     /locus_tag="ACG8H7_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984582.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1756 a          816 c          787 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttgg gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAZKAS010000164         4777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000164 DAZKAS010000000
VERSION     DAZKAS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.168"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..1228
                     /locus_tag="ACG8H7_002527"
     CDS             38..1228
                     /locus_tag="ACG8H7_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFP8984583.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1362..3551
                     /locus_tag="ACG8H7_002528"
     CDS             1362..3551
                     /locus_tag="ACG8H7_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8984584.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3548..>4777
                     /locus_tag="ACG8H7_002529"
     CDS             3548..>4777
                     /locus_tag="ACG8H7_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP8984585.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT         1716 a          735 c          862 g         1464 t
ORIGIN      
        1 gttaccacaa cacattcaca gaagagagga cttccctatg aacgatttta ctacagaaat
       61 tctaaagact ctagcgaaca aaggcgattt gaatgaatta ttccgtgtcc atttggagaa
      121 agcagtcaat acgcttctca aaacggagtt aacggctttc ctagattacg aaaagtatga
      181 tcgcattggt tttaacacgg gtaattctcg taacggctcc tatgaccgta cggtcaagac
      241 cgagtatggg gaacttcatc tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca
      301 gactgttcct gcttatagac ggacgaatga cacgttagag gagaccgtca ttcacctctt
      361 ccgaaaaggt attaccatgt cggaaatcgc agacttgatt gagaaaatgt atgggcatta
      421 ctacacgccc caaaccatgt ccaatataac aaaatcattt acagaagagg taacggcgtt
      481 taatgggcgg gagcttcatg accgttatgc tgctatttat atggacgcaa cgtatattcc
      541 gttaaagcgg aaaaccgtcg ccaaggaagc tattcatatc gcagttggca ttcgcccgga
      601 cggatcaaag gaagtattga gctatgcgat tgcaccgact gaatccatca cgatttggga
      661 ggaaatttta ttggaccttc aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga
      721 tggcttaaag gggatggtag gagcgattag tcggttctat cccaaagctc gttttcaaca
      781 ttgttgtgta cacgtttccc gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga
      841 agtctgtgat gattttaaaa tggtgtatca agcctcatct aaagaggtgg cgttggaagc
      901 acgtggtact tttgcggaaa aatggaaaac cagctatcca aaagtggttg aatcgattct
      961 ttcgaacgat cacttgctca ctttctatga tttccccttg gccatacgca agagtattta
     1021 ttctacgaac ttgattgaat cctttaataa acaaatcaag aaatacagcc accgcaagga
     1081 acagttccaa aatgaagagt cgatggaacg tttcttagtc tcgtcttttg atacttacaa
     1141 ccaaaaattc ctaggtcgca gtcataaagg tttccaacag gccgaaggcg agctcgaaca
     1201 aatgctaagc caactgattg agaattagaa acaatctgca gtagggaaga atcatttaca
     1261 caaaattatt gacgctccct tataatgaga acttttacta acataattta tgataaactt
     1321 actatataat catattaatt aaatagttag gagatctgct tatgtttata tctactcttc
     1381 aaattcgaaa ttttagaaat tatgaatccg aaatctttcg attcgataat gagacaaata
     1441 ctattatagg tgagaatgat tcagggaaaa ccaatgctct tactgcattg agaatcttat
     1501 tagatgatag ttattactat agtagtaaaa cactaaaaga atcagatttt tttcatggaa
     1561 ttcaaaatgg gtggcaaggg cattggatta ttatctctgc gacatttgaa ggaatcagcg
     1621 aagaagaatt tgacaatgag atttgtgctt ccatttcttt aaattctgaa agtcaaacaa
     1681 tcttagaaga attgatttca aatgcagata aaggtgtagg tagtatttct ctatttatta
     1741 gacctaacaa agctattaga aagcaattat ttgatatttc cactgaatta gactctcatc
     1801 aatttaacga atttagaaat tctattcgtc tatccgacta tgaattttac tacacatcta
     1861 aatcaaatct aaatttctgt atcaatgaaa attatgataa attggtagga agactcaatg
     1921 acaatatagc tagtaatcct gaagaagacg atgaagctct gttgggctca agaattgaca
     1981 tgtcagatat tttcaagcat atttcggttg tttatgttga cgctcttcga gatgttctta
     2041 gagaaatgaa aaacaatcga aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa
     2101 tatcatcaga taatgttgag tcattaaaaa caattataca gcaattaaat gagactatta
     2161 ccagtgttcc tgaaattagg aaaattggcg aaaatattaa tcggcaacta aatagcataa
     2221 ttggttcagt gtattctcct aatcttcttc tttcctcaac tatgtcagat gatatgggtt
     2281 ctttagcaaa atttctttca atgaagccag aacaaaatat cgacttagac ttactagggt
     2341 taggacatct aaatatgatt tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt
     2401 caagagaact acttaatatt atgttgattg aagaaccaga agctcatatt catcatcata
     2461 ttcaaaaaac tctgttcgaa ggattaaatc ttcaaaaaaa ctatactcag attctcatga
     2521 ccacacattc tgtacattta gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat
     2581 cgtttaatgg caaatctatc gttatgaatc cagcaaataa tctgaactca tttgctaaag
     2641 aaaagttaaa aaagaacaat ctaaatctaa taaacgcagt agagagatat ttagatgtta
     2701 aaagaaatgg attgcttttt tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa
     2761 tacttattcc tcaaatagta aaaaaattat ttggtataaa cttagacgaa cttggaatag
     2821 gtttgattaa tgttggtagt acttcgtttg aatatatcgc tagcttgttt gacgatgata
     2881 gaatacagag aaagtgtgct attgtaactg atttagatga acaggcaatc ccatcagaac
     2941 atcgattgta taaaagcact gcacaagaga aaggtcaagc aagaaaagaa aaattatcct
     3001 ccctttttaa tgaaaacaat tgggttaatt gtttttatgc taatacaaca tttgaaattg
     3061 agtttttaga tgtgaataaa gaaaatattc taacttatgt cagtccaatt atagatgacg
     3121 cattcaaaga taaaaaaaca ataactgctt ataaagaaga aatacaaagt gaacaatatt
     3181 tagaaagaaa tttagcgatg ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa
     3241 ttgagttagg tgaaaaaatt gactttttaa tcaaaattcc taagtatatt cttaatgcta
     3301 ttgcttttgc atgtcaggaa atagtagatc tctcaattta taaaaaaatt ctactccata
     3361 caatcgactt gtacgaggaa actacagaat tgtcagcttt atcagctagt ttaaaaacaa
     3421 gtgatacagc tgatttgaat atagcaataa tttctttatt agagtacttt ggtaaagatg
     3481 aggatgttgt gtatctaatc aatcagatag aaaattacac aaaaatactt tatgctgagg
     3541 tttcaaaatg acaaatatct tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga
     3601 tgttgtggaa agtgaagata acctttatgt aaatgcatgt cctgggagtg ggaaaacaag
     3661 ggtacttaca aggaaaattg cttaccaagc aattaaacac gatggatcac taaaaagaat
     3721 aattgctatc acatacacta atagagctgc tgaagaaatt aaggaacgcc taagtttact
     3781 tagtatagat gatgatatta atatttgggt ggggacaata catcagtttt gtcttgaatt
     3841 tattatttat ccttttaaaa tgaatctctc aagagtatca aaaggattta caattattga
     3901 tgattatact caaagagctt acgtcagcca aattaataat atgctgaatt taaatctcaa
     3961 aacgtatgaa ctgaacaaaa ttgatttgtc actttctcat gattttaaaa ttaatgaagt
     4021 tcagtatact caagtttctg aaatgtatca cactatgttg gaaaaaaata aagaaattga
     4081 ttttgatatg attttactag tagcgtataa aattttaact gtaaacagta acgttgcacg
     4141 taatattgct agtaatataa gggcgattta tgtggatgaa tttcaagata ctagagaatt
     4201 acaatacaat gtaatagcta aattacttca aaacaaccct caaattcagt caatgtttgt
     4261 tggagatatt gaccaagcaa tttatggatc attagatggt atagctaaat cagttgaaga
     4321 actagaaatg ctaactagtc acacttttca atcaaagacg ctacatggct gttacaggtc
     4381 taatcagaga ttagtagact tctactctaa ttttcagagt tgtccttacg agattgaaag
     4441 cagagggaat aataaaaacg accgtggttt tataagttat gattgcaaag ttactaagga
     4501 ggatttaccc aacataataa aaaatataat aaaggaaaag attgaaagcg gcattcatga
     4561 aaaagatatt tgtgtacttg ctcctcaaca ctatcctctg ttttcactgg gtgagtattt
     4621 aaagaaagaa ttaccgtatt gcaattttga tgctataaat atatcaccaa ttaaggtaaa
     4681 taatcatagt ttatttttca agttagcaat tttgtacttt actgaatcag gtgaaaaggt
     4741 tcgaagaaga aagctaattg caaacgatat tttaatt
//
LOCUS       DAZKAS010000165         4768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000165 DAZKAS010000000
VERSION     DAZKAS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.169"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1118
                     /locus_tag="ACG8H7_002530"
     CDS             72..1118
                     /locus_tag="ACG8H7_002530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP8984586.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="ACG8H7_002531"
     CDS             1135..2358
                     /locus_tag="ACG8H7_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP8984587.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="ACG8H7_002532"
     CDS             2447..3334
                     /locus_tag="ACG8H7_002532"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP8984588.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="ACG8H7_002533"
     CDS             3420..3650
                     /locus_tag="ACG8H7_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFP8984589.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="ACG8H7_002534"
     CDS             complement(3707..4672)
                     /locus_tag="ACG8H7_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP8984590.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1613 a          792 c          969 g         1394 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctatag ttctgttcac agaaaata
//
LOCUS       DAZKAS010000166         4697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000166 DAZKAS010000000
VERSION     DAZKAS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.170"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG8H7_002535"
     CDS             <1..649
                     /locus_tag="ACG8H7_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984591.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACG8H7_002536"
     CDS             708..1148
                     /locus_tag="ACG8H7_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984592.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACG8H7_002537"
     CDS             1160..1828
                     /locus_tag="ACG8H7_002537"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP8984593.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACG8H7_002538"
     CDS             1892..2515
                     /locus_tag="ACG8H7_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFP8984594.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACG8H7_002539"
     CDS             2571..2957
                     /locus_tag="ACG8H7_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFP8984595.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACG8H7_002540"
     CDS             3170..4561
                     /locus_tag="ACG8H7_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFP8984596.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1559 a          782 c         1000 g         1356 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttg
//
LOCUS       DAZKAS010000167         4652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000167 DAZKAS010000000
VERSION     DAZKAS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.171"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(375..1328)
                     /locus_tag="ACG8H7_002541"
     CDS             complement(375..1328)
                     /locus_tag="ACG8H7_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP8984597.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1351..2106)
                     /locus_tag="ACG8H7_002542"
     CDS             complement(1351..2106)
                     /locus_tag="ACG8H7_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984598.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2103..3062)
                     /locus_tag="ACG8H7_002543"
     CDS             complement(2103..3062)
                     /locus_tag="ACG8H7_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP8984599.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3055..4005)
                     /locus_tag="ACG8H7_002544"
     CDS             complement(3055..4005)
                     /locus_tag="ACG8H7_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFP8984600.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4155..4607
                     /locus_tag="ACG8H7_002545"
     CDS             4155..4607
                     /locus_tag="ACG8H7_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFP8984601.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
BASE COUNT         1528 a          964 c          784 g         1376 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa
       61 aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga
      121 ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat
      181 caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa
      241 accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat
      301 aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc
      361 ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat
      421 tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt
      481 ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct
      541 ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca
      601 tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca
      661 aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc
      721 ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc
      781 tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag
      841 atctccccta aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa
      901 gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca
      961 gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt
     1021 ccacctgctg attctacttt tttataggat gaaagatact caggaaggct gcttgtagct
     1081 gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg
     1141 aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt
     1201 atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg
     1261 ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat
     1321 tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc
     1381 aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat
     1441 ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc
     1501 ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg
     1561 tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa
     1621 aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg
     1681 ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag
     1741 gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata
     1801 aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga
     1861 ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt
     1921 atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac
     1981 cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt
     2041 taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc
     2101 aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca
     2161 aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt
     2221 cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga
     2281 taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga
     2341 cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca
     2401 aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct
     2461 agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc
     2521 aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca
     2581 tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct
     2641 aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag
     2701 aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta
     2761 cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg
     2821 acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc
     2881 tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc
     2941 ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt
     3001 cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc
     3061 atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat
     3121 cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac
     3181 taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata
     3241 tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat
     3301 caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta
     3361 atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc
     3421 aaatagccaa accaagagca acaatggaac aacagcatac aataactcat aatttcctcg
     3481 tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg
     3541 ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg
     3601 aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc
     3661 tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa
     3721 aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga
     3781 aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc
     3841 cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg
     3901 cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat
     3961 actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct
     4021 aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt
     4081 tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa
     4141 ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg
     4201 gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct
     4261 gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa
     4321 aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg
     4381 tccaaaacca atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg
     4441 attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa
     4501 atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa
     4561 aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac
     4621 ataaattttt acatgcaaaa aaagcttttg at
//
LOCUS       DAZKAS010000168         4642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000168 DAZKAS010000000
VERSION     DAZKAS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.172"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1574
                     /gene="gadC"
                     /locus_tag="ACG8H7_002546"
     CDS             63..1574
                     /gene="gadC"
                     /locus_tag="ACG8H7_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFP8984602.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFDLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1587..2987
                     /locus_tag="ACG8H7_002547"
     CDS             1587..2987
                     /locus_tag="ACG8H7_002547"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP8984603.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3152..4592)
                     /locus_tag="ACG8H7_002548"
                     /pseudo
     CDS             complement(3152..4592)
                     /locus_tag="ACG8H7_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1451 a          768 c          878 g         1545 t
ORIGIN      
        1 ataagcgatt ttccataacc ttggtaggaa tgacaaatta aataggaaag ggaagtaata
       61 atatgaataa aaaaaagtta tctctattcg gtttttttgc cctgacagct tccatggttt
      121 tgactgttta tgagtatccg acctttgcaa catcacaatt acatttggtt ttctttcttc
      181 ttttgggagg tattttatgg tttctaccag ttgctttatg tgctgcagaa atggcaacag
      241 ttgaaggatg gaaagatggt gggattttca gttgggttag tcaaacttta ggagaacgtt
      301 ttggttttgc agctattttt ttccaatggt ttcaaattac agtaggtttt attacaatga
      361 tctacttcat actaggagcg ctgtcgtatg taattaattt tccagagctt aatacagatc
      421 catttatcaa gtttattggt gtattagtca ttttctgggt actgactttt tcacagttgg
      481 gcgggaccaa tcgtaccgct aaaattgcta aagcaggatt tgtcattgga attgttattc
      541 cttcgatcat tctctttgat ttagcagcag cctactttat cggcggtaat ccgattcaaa
      601 tggctattaa tgcgaaagct tttattcctg acttttctca gctttcaact ttagtagtat
      661 ttgtatcttt tatattggct tatatgggcg tagaagcttc agcatcacat attaatgagc
      721 tggaaaatcc ccaaagaaac tacccattgg caatgttttt actagtgatt ttggccatcg
      781 ccttagatgc aatcggcggt ttctcagtag cagcggttat tccacaacat gacttgtctc
      841 tcagtgctgg tgtaatccaa acatttgaaa cgcttatttt acattttaac agtcatttac
      901 attggttggt aaaagtgatt gctctcatga ttgcctgtgg cgttatgggt gaagttagtt
      961 cttgggtagt tggtccatca cgagggatgt ttgcagctgc tcagcgaggt ctgttaccag
     1021 ttttcttccg gaaaactaat aaacaagacg ttccagttcc gttgattatg atacaaggaa
     1081 ttatcgttac tatttgggca gcagtcttaa cttttggtgg aggaggcaac aatttatcat
     1141 tcttagtagc aatctcctta actgttgtca tttatctcgt aggatattta ctatttttcc
     1201 taggttactt tgtattagtt tttaagaaaa aagacttaaa acgaacttac aatgtaccag
     1261 gaaaaactgt tggcaaaacg attatcggtg gaattggttt tattttatca gtgttcgcat
     1321 tgcttatctc ttttgtacca ccagcttcaa ttgcagctag tgagcaccga acataccaaa
     1381 tcatactact tattagcttt gtagtgactg ttcttttacc ttttatcatt tatgaagtac
     1441 atgataaaag tgctcacaca actattgagg aacctactca cgtgaaagct gaagacgtta
     1501 atcccgctgt ttatccagct gctagaggag agcaccacgt tacaaaaaat gatgaacata
     1561 ttttaaaaca ttaggaggaa ttcattatgt tatacggaaa agataatcaa gaagaaaaaa
     1621 actatttgga accaattttt ggctctgcaa gtgaggatgt tgacttgcct aaatataagt
     1681 taaacaaaga atccattgaa ccacgaattg cttatcaatt agtacaagac gagatgttgg
     1741 atgaaggaaa tgcgcgatta aacttagcta ctttttgtca aacgtatatg gaacctgaag
     1801 cagtgaaatt gatgacccaa acgttagaaa aaaatgcaat tgataaatca gaatacccac
     1861 gaacaacgga aattgaaaac cgctgtgtaa atatgattgc tgatttatgg catgctccaa
     1921 ataatgaaaa attcatggga acttcaacga tcggctcttc agaagcctgc atgctgggtg
     1981 gtatggccat gaaatttgct tggcgtaaac gtgctgaaaa attaggtctt gatattcaag
     2041 caaaaaaacc taacctggtg atctcttctg gttaccaagt ttgttgggaa aaattctgtg
     2101 tatattggga tgtggaactg agagaagtcc caatggatga aaaacatatg tcaattaatc
     2161 tagatactgt catggattat gtggatgagt acacaattgg tattgtaggt attatgggta
     2221 ttacttacac tggtcgttat gatgatatca agggtctgaa tgatttagtt gaagctcaca
     2281 ataaacaaac tgactataaa gtatacattc atgttgacgc tgcatcgggt ggcttttatg
     2341 caccatttac tgaacctgat ctagtttggg attttcaatt gaaaaatgtt atctcaatta
     2401 attcttcagg tcacaaatat ggtttggtat atccaggtgt gggttgggtc ttatggcgtg
     2461 accaacaata cttaccagaa gaattagtat ttaaagttag ttacttaggt ggagaaatgc
     2521 caactatggc tatcaacttc tctcatagtg cagcacaact aattgggcaa tactacaact
     2581 ttgtacgcta tggctttgat ggttatcgtg atattcacca aagaactcat gatgttgctg
     2641 tttatttagc caaagagatc gaaaaaactg gtatttttga aatcattaat gatggatcag
     2701 aattgccagt tgtgtgctat aagctgaaag aagatcccaa tcgcgaatgg acactatatg
     2761 atttatctga tcgtctgtta atgaagggat ggcaagtccc agcctaccca ttacctaaag
     2821 acttggatca attaattatt caacgcttag ttgttcgagc agactttgga atgaacatgg
     2881 ctggtgatta tgtacaagat atgaaccaag caattgaaga gttgaataaa gctcatattg
     2941 tttatcataa aaaacaggat gtgaaaacgt acggctttac tcactaaaat tcttcagaga
     3001 aatataaaag aagttaatta actaataaaa ggagttgtga ttaccacaca gctccttaaa
     3061 tctatttgaa taaatatatg atattcattt gttacactct caatcttagc tgttgttcta
     3121 ttcatcatta ctcttattgt aagtagttca gtcaaatttg cttgttcaat tgatcgataa
     3181 cagtatgaat tgtttgaaaa gtaatcggat atataattgt tttctcacta cctaagcctt
     3241 cttggtataa agaactatcg caaagtataa tccgctgagt acaaaagaaa gtgtcgtcta
     3301 ctatttctct aagttgttta caaatgatat tactaataga aaagtgttct cccaaaatgt
     3361 cttctaactg ttgtcgatag tataaatacc taaagtctga cggagctact atgaacactt
     3421 caattggaga tttccgttta tgctcattta aaagacttaa actaatggtt agtttacgaa
     3481 tagaattctc attccaacgc agaaataaat tgttttgttt tcgccattgt tcaagaattt
     3541 catgaacttc taagtacaaa gcgcgttgct cttcatttaa caagtaagtc ttatcaggtt
     3601 gaaatatctg aatatcagcc caaagtgagc ggataaacgg gagaagtgct ttatcaaaaa
     3661 gtatctgatt ttcttcagat atattcagag ataagataag tttttgactg agctcaatca
     3721 ccaaagggtt atctgctaca tggttttgat aaacaacgtt caagtctttt tgtaacaatt
     3781 caaagttatc gcatagataa tttctagagt tgaatagaaa gtagatataa aggcattcat
     3841 cttcgtcatg acataaagag aaagaaagtt gctctaatcc tttctgtatt agctgaaaca
     3901 agggagtatg tcgggcaact cttttttctt cctgactaaa atatattggg ttagtcttag
     3961 atctctgcaa accgatagcg atacctaata aaataaattg tttttcaact gtagggtaac
     4021 ttcttgaaaa aaaatgtttt tctacataat caatcaattg ttgaccgctt tgaataattg
     4081 atttatttag ctggatcaac tctgtccgat ttgtctcata gattatcgtt agtaacaaac
     4141 ttcgataatc cttttcagga atttcaaaat ggccatcagc atttaagtac tgcatttgtt
     4201 taaaaaaatt aatcaactca tttcgtaagt tgtaaacttt gcttaacgaa atgaaggtag
     4261 tatcagctat attactccaa ttgtgttccc ctgataaata atgtcctagt acattgaaaa
     4321 agtcagagcg ctgatagatg agttgagtga gtgccaacct cgaaaacgaa gagtcgaatg
     4381 cgactgtaaa gctacttcta gatttctcca ctgaaataat ttgctgatgg agtatgtcgg
     4441 taatgcattc caagtcgtta gtgacagaca gtgagtgggc aaacgttcaa ttgatctgct
     4501 atttcgcgaa tctgaatggt ccctttttcg tgtaattgct cacaaatgta aacttgacga
     4561 aaaatacttt tctccagata ggtttccaac atagtactcc tcctagtcat tttatacata
     4621 actattataa ctctaccaat cg
//
LOCUS       DAZKAS010000169         4635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000169 DAZKAS010000000
VERSION     DAZKAS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.173"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..519
                     /locus_tag="ACG8H7_002549"
     CDS             175..519
                     /locus_tag="ACG8H7_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP8984604.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="ACG8H7_002550"
     CDS             520..939
                     /locus_tag="ACG8H7_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP8984605.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="ACG8H7_002551"
     CDS             complement(1031..1381)
                     /locus_tag="ACG8H7_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984606.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2677)
                     /locus_tag="ACG8H7_002552"
                     /pseudo
     CDS             complement(1347..2677)
                     /locus_tag="ACG8H7_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            2933..3499
                     /locus_tag="ACG8H7_002553"
     CDS             2933..3499
                     /locus_tag="ACG8H7_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP8984607.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="ACG8H7_002554"
     tRNA            3644..3731
                     /locus_tag="ACG8H7_002554"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4635
                     /locus_tag="ACG8H7_002555"
     CDS             4065..>4635
                     /locus_tag="ACG8H7_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFP8984608.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1685 a          778 c          891 g         1281 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tggaa
//
LOCUS       DAZKAS010000170         4615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000170 DAZKAS010000000
VERSION     DAZKAS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.174"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1068)
                     /gene="lacD"
                     /locus_tag="ACG8H7_002556"
     CDS             complement(94..1068)
                     /gene="lacD"
                     /locus_tag="ACG8H7_002556"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFP8984609.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(1065..1523)
                     /locus_tag="ACG8H7_002557"
     CDS             complement(1065..1523)
                     /locus_tag="ACG8H7_002557"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP8984610.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1520..2983)
                     /locus_tag="ACG8H7_002558"
     CDS             complement(1520..2983)
                     /locus_tag="ACG8H7_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP8984611.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2965..3897)
                     /gene="pfkB"
                     /locus_tag="ACG8H7_002559"
     CDS             complement(2965..3897)
                     /gene="pfkB"
                     /locus_tag="ACG8H7_002559"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP8984612.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            4039..>4615
                     /locus_tag="ACG8H7_002560"
     CDS             4039..>4615
                     /locus_tag="ACG8H7_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP8984613.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         1382 a         1031 c          730 g         1472 t
ORIGIN      
        1 tgcagtttta tacttttttt ctataaaatt tgacaaacat ttttttaaat aactaattat
       61 tttttgaatc tgtcactcag acaatccttt ctcttacttg ttaacataaa tagaagttgc
      121 tgtttcttct aatagtgtac ttaattcttt aatattttgt tttccttctt ctaacatcca
      181 actactcgct ttttcttcgc cttcggtaac aaaaggtgct actcccttcg cccatgttgc
      241 tcgcccacat aagacaccat tgaatgtcga acctgattgt tttgcaaaac gcaatgtatc
      301 ttgaaatagt gcagcgctga ctcccgcact taaaaaaata aacggttgtt cagtcacttt
      361 cgattgttca tagaaatacg cggatgcctc tgcttgacta taacaagtat cttgcccata
      421 tccttccaca aatctcatat tcactggtac ttctactttc aatacatcca cttgataacg
      481 ttcttttgaa aattctcgca tggcttcaat cactttatgc ggttttactt ttgcatagtc
      541 aagagaagac ggatcgccaa tagttgcatc gtaagaaagg agctctaaaa agaaaggtat
      601 gccttctgct tcgcattctg aaccaatccg ttctataact atttgttttt gcacattaat
      661 ctctactggt tcatcaacgt catagtacaa taaaaattta caagcatccg cacccacttc
      721 ttttaacctt ttcactgacc aactattcag taaatctggt agtcggcctg gcattttcgc
      781 atcatagccg gttttttcat aagctagcaa caaaccacat tctttctctc gaagcgctgc
      841 tgcaggcaga ccatattctg gatcgagtaa aatagcggaa gcatacggag tcaggtactg
      901 agaaaccatc tgtttaaatt ggatgatatc ttctgatgta gcttctttat attggttgat
      961 cattttcttc aaagcaccac gttggtcgat agctagcgcg ctaataattc cagcttcatc
     1021 cgtcaatcgc ttcaaacgtt cctgtttttc tatactcatt ttcttcatat atcttcacct
     1081 atttctttag ttaatattgt agctatttct atttttgatt ttgcagctct caactgttct
     1141 gtaacctctt ttcgcatcaa tgatcgtgca acgagagaca ataatttcaa atgagtcgtt
     1201 cctgcttctg aatctgggat aaataacgca aaaataaaat caactgactg tctatctaaa
     1261 ctgttccatt caattccttt tttcagtgtg acgataatca aactaggttc attgattgtt
     1321 tgagacttcg catgagggat agcaaaacct gacattattc ctgtagtacc ttctttttct
     1381 ctttccagaa gtttttgata tacttctttt tcgtcatgag cgattcctaa acagaatgtt
     1441 tgctttgcta aaaacttaaa tgcttcttct tgattatcta atggctgttg taaaaaaatg
     1501 ttctcttcat gaatgattgt catgataaac ctcctgctct tccttcttcg ataactgtac
     1561 gatttgttgc tttattatcc ctcttcttgt ctttatcagt tactcttttc ttttggatca
     1621 aacctagtat cactccacca acgatcgaac caataagaat cgctgtaacc cattggattt
     1681 ttcccgtcac tacaggtaac actaaaaaac cgccatgtgg ggctggtact tggacaccaa
     1741 acagataggt caagacagca gcaattgatg agcctaacat catcgttggt aatactcgga
     1801 tcgggtcttt cgcagcaaac ggaatagctc cttccgtaat atgagttgat cctaaaataa
     1861 aattaaccaa acctgcatta cgatcattag tatcaaaata tttaccaaac aataaagtag
     1921 caaaaccagt aatcaacggt ggtgcaatgc aagctgctga cgcaccagcc ataaaagtcg
     1981 tgtttccttg tgtcaaaaga gctgttcctg ttacatatgc cgccttatta accggtcctc
     2041 ccatatcaaa cgcacacata cagccaacaa tcacgccaag aacaagtgga ttcgaatatt
     2101 caaatccagc taaaaagctc atcattcctt catttatagc cttcattgga cctgccagca
     2161 aagacatcac gatgccaata aatgctacac caataactgg atataagacg attgccttca
     2221 agccatctag ttgtttaggt aaaattatca gtagcttttg caaaaataag ataaaataac
     2281 ctgctaagaa cccagctaaa atacctccta aaaatccagt accgccattc atggctacga
     2341 ttccaccaac aaatccaacg actaaccctg gacgtttcgc gatcccttct gcaatataag
     2401 ctgctaaaac tggaaccatc attcccattg ctgcatcgtc aactgctttt accattgccg
     2461 ctgtagcatt gtattgtgca tgatctggat cagaagaata gattccccaa acggcaaatg
     2521 aaatagcaat caatactccg ccagctacca cgaaaggcaa catatgagag acaccgttca
     2581 tcaaattttt atagatgcta cgaccaaaat tagcaccact tatcgcttct ggttctaaag
     2641 tatcagcagc ttcattatct gctaagacag taccttttcc tgccaatgct tcgtcaatca
     2701 gccgttgtgc atccttgatt cctgtactga cagaaacatc aatgactcgt tttcccacaa
     2761 atctttctga atggacatct ttgtctgcgg caataatgac agcatctgcc tgtttgattt
     2821 cttcagccgt taattcattc tcgatcccaa tttgtccatg ggtctccact ttgatcgtga
     2881 tgcctttttt cctagctgct tgctctaatg cctcttgcgc catgtaggtg tgcgcaattc
     2941 ctgtcggaca accggttgct gcaattagtt gatattttcc catctttcga acctctcttc
     3001 cttgattttg atttgtttac ttaattcctc cacatccaaa aaatcagtca gtcctttacg
     3061 aaaagcagta gagctgcctg ctgctatgct tttacgtaac gtttcatcaa gcgatcgtcg
     3121 attcatgtat cctgctaaaa aagtgcctaa catggcatca cctgcacatg ctgtattgac
     3181 aacttttcct tttggcgagt ttcctcgaat caccctgtct ttcgtaaaaa gaatcgctcc
     3241 tttctcaccg agggataaca aaacattttc tgcgcctgac tctactagtc gttgtccata
     3301 aacaaagtaa tcttcatctg taaccatttt tcgtccgaac caagaagaaa gttcttcctc
     3361 atttggtttc agcaaaaacg gtcgataggg taaacaatcc atgacttctt gatcactact
     3421 atcaataatc aaacaaatcc gactgatttc tattaaaacc tttggagaca gccctcttgg
     3481 taaactccca gaaacacaaa gataatcccc tgcttgtaat gctcttattt gatttaaaaa
     3541 attatgaaca gctgtctgcg gtatttcagg tccttgattc actaatttgt attcttctcc
     3601 cgtttgattg acttgtgtaa atacattgat acgcgtcata ccaggcactt caataaactc
     3661 tgtttggatt tgtttctgct gtaaatagtc ttctatatac tttcctgtga acccagcaga
     3721 aaaacctaat gccgtacttg ggaaatctag catctttaat actagtgaca cattcacccc
     3781 ttttccgttt gcctgaatgt catcctcttt tgtgcggttt accataaaag gatgcatctc
     3841 atctgtttca ataaatagat caattgccaa attcatcgtg catgtgtaga tagacataac
     3901 caacgacctc cttgattcta ttatgaaata cgcgaaatag aaaatgtttt cttaatcgtc
     3961 ccaacacaaa gaaaaccttt acatttataa actcgtcgtg ctttttttaa ctaacttttg
     4021 gtgaaagaag gaaagaatat gaatcacaaa ttaagtgaaa cagagcagta cctctggaac
     4081 tttatcgaac acctcatcct agagatccct aactattcca tcgtgaaact aagtgaacaa
     4141 gcaaatgtct caacagccac cattgtgcgg acaatgaaaa agaaaggcta tgaagggttt
     4201 acttcgttta agcatcatct aaaagaagga gaaaacaaga acatcaattt ttcattttta
     4261 gataaagtag acaaaggaat ccgccgagcc attctaaaaa atgaacaaga agtggtacgt
     4321 acaatcaata tgttggaaat cgggaacatt gaagatgcaa tacaaagaat caaatttgct
     4381 gaccgggttt ttgtctttgc ccgaggattt tcagaaatga ttggccaaga aatgctggta
     4441 aaacttcaat taacgggaaa aaattgtcag atgcatactg atccagaaat tatcaaaagc
     4501 atcagtcgga aactaactaa gaaagacagc gttatttttg tttctttgaa cggtgagaaa
     4561 acaaataaaa acatcaaaaa taagatttat gtctttattt ttcaattttt taaat
//
LOCUS       DAZKAS010000171         4609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000171 DAZKAS010000000
VERSION     DAZKAS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.175"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(84..398)
                     /locus_tag="ACG8H7_002561"
     CDS             complement(84..398)
                     /locus_tag="ACG8H7_002561"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8984614.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(449..703)
                     /locus_tag="ACG8H7_002562"
     CDS             complement(449..703)
                     /locus_tag="ACG8H7_002562"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8984615.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACG8H7_002563"
     CDS             complement(896..1171)
                     /gene="prgO"
                     /locus_tag="ACG8H7_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFP8984616.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1143..2096)
                     /locus_tag="ACG8H7_002564"
     CDS             complement(1143..2096)
                     /locus_tag="ACG8H7_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP8984617.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2708..4201
                     /locus_tag="ACG8H7_002565"
     CDS             2708..4201
                     /locus_tag="ACG8H7_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFP8984618.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4336..>4609
                     /locus_tag="ACG8H7_002566"
     CDS             4336..>4609
                     /locus_tag="ACG8H7_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFP8984619.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1541 t
ORIGIN      
        1 gcttgaattc ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta
       61 tgttttcttc tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatg
      121 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
      181 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      241 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      301 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      361 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      421 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      481 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      541 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      601 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      661 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      721 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      781 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      841 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      901 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      961 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
     1021 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
     1081 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1141 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1201 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1261 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1321 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1381 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1441 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1501 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1561 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1621 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1681 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1741 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1801 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1861 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1921 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1981 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     2041 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     2101 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2161 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2221 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2281 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2341 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2401 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2461 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2521 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2581 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2641 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2701 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2761 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2821 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2881 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2941 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     3001 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     3061 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3121 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3181 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3241 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3301 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3361 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3421 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3481 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3541 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3601 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3661 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3721 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3781 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3841 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3901 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3961 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     4021 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     4081 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4141 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4201 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4261 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4321 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4381 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4441 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4501 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4561 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaa
//
LOCUS       DAZKAS010000172         4587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000172 DAZKAS010000000
VERSION     DAZKAS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.176"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..1552
                     /locus_tag="ACG8H7_002567"
     CDS             251..1552
                     /locus_tag="ACG8H7_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP8984620.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1579..1839
                     /locus_tag="ACG8H7_002568"
     CDS             1579..1839
                     /locus_tag="ACG8H7_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP8984621.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2838..4073)
                     /locus_tag="ACG8H7_002569"
     CDS             complement(2838..4073)
                     /locus_tag="ACG8H7_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP8984622.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1352 a          711 c          917 g         1607 t
ORIGIN      
        1 aatttacaaa atttttttga aatgatatgt ttcaatcact agatttaatc attttgattt
       61 ttgttaatta tcgaattttt ttgttgctcc tatgatttag aaaacttatt ttcaggattc
      121 ttaaaaataa atttgattat ttaagagaat atattgaaat aaaatttctt aattatataa
      181 ttagttatga acattctttg cgttgtcatg caatgaatgg ttcatatttt attgaaaagg
      241 agtggtttta gtggaaaagc tgatgaaatg ggtcgaagag aaacttgtcc caccaatggc
      301 aaaaattggt acacagcgtc atttattagc aattcgtaat ggggttgttt caacattgtc
      361 attgattttg attgggacat tttttatggt ctttatcaat ttaccgtttc ctggatggaa
      421 tgaatttatt gccccttatt cggcaacgat tgttttaccc tttaggatga cgatgggatt
      481 gatggctatt tatgcaactt ttgttatggg ttcggatcta gctaaatcat atggattaga
      541 tagtgtgaca ggtggtatat tatctttagg aacgttcttt atgttacaag ttcctgtaaa
      601 cgttttaact cctgaagagg cacctcttgg ctgggtctta cctatgagtt ctttgggagc
      661 atctgggatg tttgcgggga tcttatctat gatttttgca gtggaagtat atcgtttctt
      721 taaaaatcga aatattacta tcaaaatgcc agaacaagtg ccacctgctg ttgcccgtag
      781 ttttgaggca ttgattccag gtgcagtcat tttaacaatt acttggctga ttcgaagcgt
      841 cgcgggcttt gatgtaaacg ctgccttatt atcgttattc gagcctttaa ccaatatttt
      901 agggaataat ttgcttggtg ttttactacc aatgtttttg atccatttgt tgtggtcatt
      961 tggtatccat ggcatgagta ttgtaggggc tgttgttcgt ccaatgtggc tgatcatgtt
     1021 ggatgagaat gcaaaggcat tggcagatgg tacagctgcc acccaattac cctatatcgc
     1081 tcctgaacag ttttatcaat ggacggtaac aattggtggt gcaggagcaa cgatcgtggt
     1141 atctgttttg tttatattct tctgtaaatc aaaattcttg aaagaggttg gtcgtttttc
     1201 tattattcct ggtatcttca atatcaatga gcccataatt tttggagctc ctatgatgtt
     1261 gaatccttac atgttcatcc cgttcaactt agttccttta gttttgacta ttatcagtta
     1321 ttttgcagtc aaattagaaa tggtcaatag atttactgtt ttgccaacat ggacattacc
     1381 agctccaatc ggcggttatc tttcagcagg aaatgactgg cgtgttgtta tcttgatcat
     1441 gatcaacaca ttgatagcat ttattatcta ttatccattt gtaaaagctt atgataaaaa
     1501 gatgctgtca gatgaacaaa gtcgtggaga aaacgaagtc cagactgtct aagtttggtt
     1561 tttagaaggt gggaaacaat gtattggctg attttatttt ttgtttttat ctttttatta
     1621 acggctagtc accttatatt gaatatgcta gctacatatc atattcaaat caatcgatgg
     1681 atttgggcac tggcatcatt tttgatcgtg attcttccaa agatcattgt accgcatatg
     1741 aatgttttgt tttcatgggg gacatatgta ctttgtggaa tttttgctat caattttatg
     1801 atcgagcagc atagatggtt tgtcacatcg aagctttaaa tgttctaaaa gtggttgtaa
     1861 cagaagtggt tagcttcaag aaataagaag aaatttacga aaattgcttc ttaaattttt
     1921 gtgaaatgtt ggcttatttt tcgtttggtt gaacttatta ccgcagatgc aagcttttga
     1981 ccaccgttta ttcggaatat gggactgtga caaggctttt gtcacagccc ttttttacat
     2041 aaagattatt attgttttaa ctgttttggc agaaagaagg aggaaaaaat ggaaatttta
     2101 tcagcaatgg atttagaatt agtagatcta ctaccaaagt gatcaagtca aatatagatt
     2161 tgggagctta caaccatata gtaggggctg cagtatggtg tatgaatggt aatatgtatg
     2221 atgtgagtgt ttattcaaat catgaaacgt gtatagaaat aattgtgctg ggaatagcaa
     2281 tcagtcatgg tgaacgtgac ttttagtgta tcgttgctga cgacatagaa cattctgaca
     2341 tgatctactc aatatgtggg gattgctggc agtttatata ggagtatgca tcggattgcg
     2401 agatcattgt ctcaacagaa gagggaggtt gattatcaaa gataaagtgg taaatcctac
     2461 atttgattta gtaattgttg aggggactag cggcacaaat ctctacaaag cgatgcttct
     2521 agatctatat gacacatttt aagaaaatgt atttgcctaa ttattatggt ctttcttttg
     2581 aagaaacgtt gagatgatcc aaccagagaa aggtgtcaaa gaatttgact aagaataaat
     2641 ggcagacgct acctatgctc atttggatag cgtcagatct tttattttaa ggatgatttt
     2701 gtgtttttta gacaccgtaa tttaagtaac tctaactttt caatccacta tttacttttt
     2761 ttgtattttc actgtctggg tctcgaatag cgaaattact gatgagtctc tattaaaaaa
     2821 gctttttcta cttgaaacta tattgggtgg ttgctcagga aggtttcgat ttcttgaatt
     2881 gcttgttgtg gaccatttat tggaaaaacg gcttgcagcg tgcgtgcctg ctgacgaatc
     2941 ttgggtgaag aaacttcgcg cagagcaatc tgcagttttc tttcagtcac tggttttgtg
     3001 agggcgattg cgtttccgta ttttacacaa tagtcaatat tacagctttg ctcaaattgc
     3061 atccccacac cgataaacgg tttgccggat tcacaggcag tttggacggt cccttccccg
     3121 ccgtggataa aagaaaaatc aatgaattca tgcaaaagat gactagacag atattctgtt
     3181 attaaaagat cttctgagtc agattgaatg tcatcaagca gatagtcaat aggtgcaata
     3241 accgctagat ccggttgttt ttgtaagaag aacaaaattt tgcgtaacat acgcggattg
     3301 cctgaactac ccattgcgaa atagataata cgttttcttt cttgttgctt tcgctgtata
     3361 aaagttagca cttcttcagg caaggaagta ctgagcttag caaaaattgg accaacgatt
     3421 cgataatttt ccggcagctg ttcttcttcc aaaaatagtt taggcgttgt gatgagattt
     3481 agatcagcat ccatcatttg taaacttgtt tttggtaaag gaagcatttc tttttgtgca
     3541 gcgtgtttga aggcattggg gatccatcgg gtatttacta tacgcttttt tgtccagtgc
     3601 caaaggggat tgcttaaagg gccgaagaaa cgtaactcgt ggggcatcag cgcatgctct
     3661 gattgaaaaa aaggttcact caaagcatag ggtttaacat atactaaagg aatcttttta
     3721 ttgcgggccg ataaaaaaat tgtcacattc gaacctgtca caatcagtcg aggctcaaac
     3781 ttagcaatca gactctgttc actagtgata cgagaggcaa ccatttcttt ggtaaaaggg
     3841 tttttaagtg tacgcaactg gtcaaatttt atgatttgtt cttcttgttt tttcgtaagg
     3901 atcggcgcta gtaaatgata ctcaaagcca ctttctgtga tcaaaggagc aaaagtttta
     3961 gaaaagccaa agaatacaca gtaataacgg tctttcatag ctttggcaac ctcaatcatt
     4021 cgtgttgtct cagctaaatt gaatactgca ggagcaaaaa gaattgtctt catgaaatcc
     4081 tccttattgg aatgagtcta tcccttcgtt tcttactagt atactaaaaa acacagcgtt
     4141 cgagtagcct atgtatattt tgaatattaa actgtctctc gctgttttca caacttctaa
     4201 atttaaattt tgtaatcaag ttgaaactcc aattttcaaa aaaacacgca acaatttttt
     4261 gatcgattta tatgttgtgc cagataaata cgagaagttg tgtcattaaa aataaacatg
     4321 agaacataaa aaaataaaat aattatatta attgataggt gtgctgtcgt cattatacac
     4381 acgtaagaaa gaaatagtta tgtttaaaaa tattcttaga ctttctcgcc atgacaattt
     4441 tcactatgtg ttataactgt aaacgataat tattcagata acacaaaaag aaatcaagga
     4501 gcagatacgc cagttgttta atttgaatat tattttaaaa ttaaaatttt tattctgttc
     4561 taaacatttt tggttgttta gaacttt
//
LOCUS       DAZKAS010000173         4566 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000173 DAZKAS010000000
VERSION     DAZKAS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.177"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..913
                     /locus_tag="ACG8H7_002570"
     CDS             59..913
                     /locus_tag="ACG8H7_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP8984623.1"
                     /translation="MENSLYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACG8H7_002571"
     CDS             1010..3259
                     /locus_tag="ACG8H7_002571"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP8984624.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..4437
                     /locus_tag="ACG8H7_002572"
     CDS             3262..4437
                     /locus_tag="ACG8H7_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8984625.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4440..>4566)
                     /locus_tag="ACG8H7_002573"
     CDS             complement(4440..>4566)
                     /locus_tag="ACG8H7_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP8984626.1"
                     /translation="FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1604 a          657 c          829 g         1476 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct ctatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acaggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagtaa cccgtggtgc tagttaattt
     4441 taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga
     4501 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     4561 ttgaaa
//
LOCUS       DAZKAS010000174         4550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000174 DAZKAS010000000
VERSION     DAZKAS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.178"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1983
                     /locus_tag="ACG8H7_002574"
     CDS             49..1983
                     /locus_tag="ACG8H7_002574"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP8984627.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2440..2856
                     /locus_tag="ACG8H7_002575"
     CDS             2440..2856
                     /locus_tag="ACG8H7_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFP8984628.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2870..3436
                     /locus_tag="ACG8H7_002576"
     CDS             2870..3436
                     /locus_tag="ACG8H7_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFP8984629.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3459..4445
                     /locus_tag="ACG8H7_002577"
     CDS             3459..4445
                     /locus_tag="ACG8H7_002577"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFP8984630.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         1415 a          848 c         1001 g         1286 t
ORIGIN      
        1 attatcatat attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa
       61 aatattgtcg ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag
      121 aaactgaaaa aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg
      181 atgaccgagt tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat
      241 ctgcagcatt tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt
      301 atcgataaag aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg
      361 atccttggaa tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga
      421 ttcacccttt ggtcatttga agattctgta aggattcggg aacatattga gaagacagta
      481 gcaaaagctg cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc
      541 tgtggttctg gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc
      601 ctagccaaag attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg
      661 ccgacgatct tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt
      721 aagcacaacg tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc
      781 aaattgaaag acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa
      841 ggaacttctg acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc
      901 aatgaatata tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta
      961 gtcttttatg aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa
     1021 acaggacaca cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa
     1081 tttaaaggaa actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac
     1141 ctatttgata agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat
     1201 ttgaaatact tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag
     1261 tttttccata tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat
     1321 gtcttatgga gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc
     1381 aaaaaaatca ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca
     1441 tggttcactg gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc
     1501 agtggtgctt ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc
     1561 ggagaagcag cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca
     1621 atggctgtga ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca
     1681 aacaatgatg tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca
     1741 ttagcattga ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg
     1801 atcacgttct gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct
     1861 ttcgcattga tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg
     1921 ctacaacgta cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa
     1981 taaatagata aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac
     2041 cgtcacagcc tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat
     2101 gagcaagaca caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga
     2161 agtagcttct tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa
     2221 attccttctt atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt
     2281 tccacctgct gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc
     2341 tattcttcgt tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc
     2401 taaaattggg tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct
     2461 gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct
     2521 tttttaccag tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg
     2581 gtactgcgtg tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc
     2641 aacacatata cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat
     2701 cgagtacgga taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca
     2761 acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca
     2821 acagatggag gtgacgaaga cagtctgatt tattagacct tcacacacca tgagtaaatt
     2881 acaaaaaatg atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga
     2941 aaatatgatc ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct
     3001 aatcacgatc gttgccattt ttaccatgaa aaaacaagac agcttcttac tactttggct
     3061 acgactattc ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag
     3121 tggtgctttg cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt
     3181 cagtatcatc ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat
     3241 ggcttctttc attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt
     3301 agggatttta tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca
     3361 gacacttcaa cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat
     3421 gtttaccaaa aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg
     3481 aaaactatcc tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg
     3541 tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga aacgatccat gccggcggaa
     3601 agctgttgcg tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag
     3661 acaaagccat cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg
     3721 atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg
     3781 gtaatgtcgt ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt
     3841 ctgattactc cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat
     3901 tgaatggcga attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat
     3961 atttggaaaa tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg
     4021 cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta
     4081 tcgggatggc ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa
     4141 tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact
     4201 ctttgcaaaa aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag
     4261 aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta
     4321 ggaagttagc ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca
     4381 cctctcttcg aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag
     4441 actgaatagg gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt
     4501 ttgacaaatt gaattgtagt tcttgtattt atgataaaaa gcaaaaataa
//
LOCUS       DAZKAS010000175         4485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000175 DAZKAS010000000
VERSION     DAZKAS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.179"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      142..308
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            456..1013
                     /locus_tag="ACG8H7_002578"
                     /pseudo
     CDS             456..1013
                     /locus_tag="ACG8H7_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1071..3110
                     /locus_tag="ACG8H7_002579"
     CDS             1071..3110
                     /locus_tag="ACG8H7_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFP8984631.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3219..4370)
                     /locus_tag="ACG8H7_002580"
     CDS             complement(3219..4370)
                     /locus_tag="ACG8H7_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP8984632.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1621 a          821 c          870 g         1173 t
ORIGIN      
        1 aatttgcaac gggattcttt caacttattt gttatttttt tctaaaaaat attaaagatt
       61 gttaaatttc actatttcat ttcaaattcc attgcctaat cattaggaaa agtctatcat
      121 gaactcgtaa atagaatata gtaataacct tgttaggtga ggctcctata caaacatagg
      181 ctgttgccca gaaacgtcga aagtcgccaa tgggtaaaac agaaactgtt ggattaaggc
      241 tttttccaat agctaagaat gttttcttta cgttgtatag tgctaaaact cggacgatga
      301 gatgatgcta ggtcagaatt cttgttcgaa aaggtttctg accttttctt tatatttttg
      361 tgactgactt atttgatgag acaaactcgc tcctcacctg acaagtggat tgctgtgtga
      421 ggaggagatg cggtgaaaaa agatcgattg gtggaggtcg ctcggattca cttctggttg
      481 cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg
      541 tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg
      601 atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta
      661 tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg
      721 atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg
      781 atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga
      841 ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt
      901 caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc
      961 cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac
     1021 aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa
     1081 gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag
     1141 cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag
     1201 ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag
     1261 ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct
     1321 tagataaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc
     1381 taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca aagatgaata
     1441 cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc
     1501 aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag
     1561 taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat
     1621 tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag
     1681 gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta
     1741 tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca
     1801 ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat
     1861 attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag
     1921 aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg
     1981 agatggcttt tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg
     2041 cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca
     2101 aattgacaat cgatgcgaag gcacaaaaaa tagcttttga cagtctagga ggaaaagctg
     2161 catcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa
     2221 gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag
     2281 aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt
     2341 ttaaaatgat tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt
     2401 tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatctttt aaaacaacgc
     2461 gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata
     2521 tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata
     2581 aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta
     2641 atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac
     2701 ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac
     2761 ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg
     2821 aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta
     2881 cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa
     2941 tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg
     3001 ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa
     3061 ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt
     3121 tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt
     3181 tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata aacttttttt
     3241 gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa
     3301 ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa
     3361 gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa
     3421 ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc
     3481 aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca
     3541 ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact
     3601 tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag
     3661 ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg
     3721 ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat
     3781 ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa
     3841 gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg
     3901 actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact
     3961 acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc
     4021 ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa
     4081 acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc
     4141 aaaagagata aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc
     4201 gaaaagtcat ggactagaat atcaggtttg acccaattca atccagcttg gcctagcaaa
     4261 acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag
     4321 ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt
     4381 cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa
     4441 tatactcaca aaaaaaccaa caatccaaaa gatttttttg gattg
//
LOCUS       DAZKAS010000176         4473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000176 DAZKAS010000000
VERSION     DAZKAS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.180"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..1052
                     /locus_tag="ACG8H7_002581"
     CDS             324..1052
                     /locus_tag="ACG8H7_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFP8984633.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="ACG8H7_002582"
     CDS             1197..3224
                     /locus_tag="ACG8H7_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFP8984634.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="ACG8H7_002583"
     CDS             3332..4429
                     /locus_tag="ACG8H7_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFP8984635.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DAZKAS010000177         4430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000177 DAZKAS010000000
VERSION     DAZKAS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.181"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG8H7_002584"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG8H7_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFP8984636.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG8H7_002585"
     CDS             complement(2020..2922)
                     /locus_tag="ACG8H7_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP8984637.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG8H7_002586"
     CDS             complement(2919..3611)
                     /locus_tag="ACG8H7_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFP8984638.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG8H7_002587"
     CDS             complement(3654..4295)
                     /locus_tag="ACG8H7_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFP8984639.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1366 a          948 c          764 g         1352 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag
//
LOCUS       DAZKAS010000178         4422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000178 DAZKAS010000000
VERSION     DAZKAS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.182"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..945)
                     /locus_tag="ACG8H7_002588"
     CDS             complement(49..945)
                     /locus_tag="ACG8H7_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8984640.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(938..1618)
                     /locus_tag="ACG8H7_002589"
     CDS             complement(938..1618)
                     /locus_tag="ACG8H7_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8984641.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1619..2401)
                     /locus_tag="ACG8H7_002590"
     CDS             complement(1619..2401)
                     /locus_tag="ACG8H7_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP8984642.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2394..3311)
                     /locus_tag="ACG8H7_002591"
     CDS             complement(2394..3311)
                     /locus_tag="ACG8H7_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8984643.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3475..3729)
                     /locus_tag="ACG8H7_002592"
     CDS             complement(3475..3729)
                     /locus_tag="ACG8H7_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984644.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1300 a          953 c          768 g         1401 t
ORIGIN      
        1 tatataagat aatacatttt tctactagtt ctcactaatc ttgtataatt aaaccctccg
       61 caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat
      121 tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg
      181 gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg
      241 tttgatagga ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt
      301 tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa
      361 catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat
      421 atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa
      481 agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa
      541 ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc
      601 ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa
      661 aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc
      721 ttgaaatgct tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag
      781 gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc
      841 agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa
      901 acttcccata aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc
      961 cccacaccgt atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa
     1021 tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa
     1081 gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt
     1141 ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca
     1201 gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat
     1261 gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat
     1321 agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa
     1381 gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa
     1441 gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt
     1501 ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca
     1561 gtaaggaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct
     1621 acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta
     1681 ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg
     1741 tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg
     1801 cgaaaacacc gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga
     1861 cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca
     1921 gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa
     1981 cttcaccaaa tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca
     2041 cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat
     2101 tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa
     2161 agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa
     2221 tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg
     2281 tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg
     2341 agacccacat tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca
     2401 tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga
     2461 ataccatcca caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca
     2521 attccttcaa aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga
     2581 gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga
     2641 gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata
     2701 ccatagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg
     2761 atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga
     2821 tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt
     2881 gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta
     2941 ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc
     3001 gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca
     3061 atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc
     3121 gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga cttacccgct
     3181 ccattttccc caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct
     3241 aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt
     3301 ccaaaatcca tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa
     3361 agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa
     3421 caatcggaca atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt
     3481 tcctgattct ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt
     3541 tgcttctttc agatcaagct tgagtaattt taccagaccg gttataagga taactatagc
     3601 aagactccct cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc
     3661 aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat
     3721 cgttgacata agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata
     3781 aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt
     3841 tgggatttac aattagctac caaataagag gttatgacaa aagacttttc acaatccctt
     3901 cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa
     3961 acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac
     4021 aacctcagaa gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt
     4081 atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa
     4141 aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc
     4201 agcattgctt cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta
     4261 acgttttgag aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc
     4321 attgaataag tttgaaaaac cttgatttat aaaggttttc ccgtaaatat attttattat
     4381 atttgattaa atttgaaatg tttgtgttct aaaatgtgtt ct
//
LOCUS       DAZKAS010000179         4354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000179 DAZKAS010000000
VERSION     DAZKAS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.183"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8H7_002593"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ACG8H7_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFP8984645.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="ACG8H7_002594"
     CDS             complement(2944..3378)
                     /locus_tag="ACG8H7_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984646.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..>4354)
                     /locus_tag="ACG8H7_002595"
     CDS             complement(3395..>4354)
                     /locus_tag="ACG8H7_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP8984647.1"
                     /translation="GPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQNLTLYSLGD
                     IRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYE
                     NKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGM
                     DIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQF
                     IGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHT
                     ISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1337 a          815 c          601 g         1601 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gacc
//
LOCUS       DAZKAS010000180         4324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000180 DAZKAS010000000
VERSION     DAZKAS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.184"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG8H7_002596"
     CDS             complement(<1..281)
                     /locus_tag="ACG8H7_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP8984648.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="ACG8H7_002597"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="ACG8H7_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="ACG8H7_002598"
     CDS             complement(2359..2721)
                     /locus_tag="ACG8H7_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984649.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="ACG8H7_002599"
     CDS             complement(2725..3033)
                     /locus_tag="ACG8H7_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984650.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="ACG8H7_002600"
     CDS             complement(3042..3461)
                     /locus_tag="ACG8H7_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984651.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="ACG8H7_002601"
     CDS             complement(3454..3687)
                     /locus_tag="ACG8H7_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984652.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="ACG8H7_002602"
     CDS             complement(3684..>4324)
                     /locus_tag="ACG8H7_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFP8984653.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DAZKAS010000181         4163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000181 DAZKAS010000000
VERSION     DAZKAS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.185"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8H7_002603"
     CDS             352..1041
                     /locus_tag="ACG8H7_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFP8984654.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8H7_002604"
     CDS             1028..2236
                     /locus_tag="ACG8H7_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFP8984655.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8H7_002605"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8H7_002605"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP8984656.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1368 a          718 c          842 g         1235 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt att
//
LOCUS       DAZKAS010000182         4054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000182 DAZKAS010000000
VERSION     DAZKAS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.186"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..1668)
                     /locus_tag="ACG8H7_002606"
     CDS             complement(163..1668)
                     /locus_tag="ACG8H7_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8984657.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1665..2351)
                     /locus_tag="ACG8H7_002607"
     CDS             complement(1665..2351)
                     /locus_tag="ACG8H7_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8984658.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2544..3965)
                     /gene="gndA"
                     /locus_tag="ACG8H7_002608"
     CDS             complement(2544..3965)
                     /gene="gndA"
                     /locus_tag="ACG8H7_002608"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP8984659.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1115 a          857 c          692 g         1390 t
ORIGIN      
        1 ttttttcgtt catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg
       61 ttcgctagtt tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt
      121 attctttgtt ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt
      181 tgcgatagga ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat
      241 acgtccttta tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc
      301 cttcgttcta gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc
      361 ttctttgggt attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga
      421 agaaatagag atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat
      481 gataatgatt aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa
      541 atcatcatct aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg
      601 ataggtgact tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc
      661 tcttgataag tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct
      721 ggcgcttaat gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact
      781 aagatgccct tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa
      841 ttgctcttgt tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc
      901 taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc
      961 tttacgtata gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga
     1021 taaaaagaaa cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt
     1081 caataatagc ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata
     1141 gccgatcttt tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt
     1201 tccttctagt gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt
     1261 ttcatgggtt ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg
     1321 ctgccctagc tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt
     1381 atgatctacg acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac
     1441 taatgtcagt tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc
     1501 tacattgttt ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc
     1561 gaaaattgta aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa
     1621 ggagggccct ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca
     1681 taacctgttc cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg
     1741 cgaagataac gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac
     1801 actttattta gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc
     1861 agcaactcgt attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg
     1921 ttttcttttt cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta
     1981 tcgccttcaa tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata
     2041 gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc
     2101 acagaatctc ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg
     2161 cagacttcta aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca
     2221 ttatttaatg ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct
     2281 tcatgcttca actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga
     2341 atattgctca tttattattt tatcctttct attctactgg ctttttaaaa actctatcat
     2401 tataccgata atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa
     2461 agagctgaag tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc
     2521 atttcctaac agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct
     2581 tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata
     2641 tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc
     2701 ggtacaggta cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat
     2761 tttttcgtga tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg
     2821 tcataggcat ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag
     2881 atttttgcga tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat
     2941 tgagcaaatc cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt
     3001 tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg
     3061 cttgcttgta cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg
     3121 atcaatggaa gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct
     3181 ttattcccag ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct
     3241 ttacgtgtca gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc
     3301 cattctttaa agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag
     3361 tcataagatt cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc
     3421 acataatgtc cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct
     3481 tttgcagaaa tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca
     3541 ggcatgattg aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata
     3601 aagttgatgc cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta
     3661 tttcctccgt caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt
     3721 gcatctgttg ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat
     3781 tctacaaatt cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact
     3841 acagcggttg ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct
     3901 atgttcaagg ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt
     3961 gacatatgaa ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac
     4021 acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZKAS010000183         4036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000183 DAZKAS010000000
VERSION     DAZKAS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.187"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..391)
                     /locus_tag="ACG8H7_002609"
     CDS             complement(242..391)
                     /locus_tag="ACG8H7_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984660.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ACG8H7_002610"
     CDS             853..1065
                     /locus_tag="ACG8H7_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984661.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ACG8H7_002611"
     CDS             complement(1343..3142)
                     /locus_tag="ACG8H7_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFP8984662.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ACG8H7_002612"
     CDS             complement(3266..3862)
                     /locus_tag="ACG8H7_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFP8984663.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAZKAS010000184         4011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000184 DAZKAS010000000
VERSION     DAZKAS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.188"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..576)
                     /locus_tag="ACG8H7_002613"
     CDS             complement(34..576)
                     /locus_tag="ACG8H7_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFP8984664.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            711..1100
                     /locus_tag="ACG8H7_002614"
     CDS             711..1100
                     /locus_tag="ACG8H7_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984665.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1104..1868
                     /locus_tag="ACG8H7_002615"
     CDS             1104..1868
                     /locus_tag="ACG8H7_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8984666.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1997..2944
                     /locus_tag="ACG8H7_002616"
     CDS             1997..2944
                     /locus_tag="ACG8H7_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8984667.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3074..3814
                     /locus_tag="ACG8H7_002617"
     CDS             3074..3814
                     /locus_tag="ACG8H7_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984668.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1330 a          684 c          814 g         1183 t
ORIGIN      
        1 atttagatat tagacagttt ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt
       61 tttagtccag aaaccacctg aaatatattt tggctcataa gaaatgataa aagcacgctc
      121 ttcgatttct ttgatcaggc gatacagttc acgttcgctt tttctaggag aaagtatttc
      181 gagaactagc cgctcacctt ccagccccat tgcagaagtc tgtgtcacac cataaccgtg
      241 ttctctcagt atacccggca aacttttatc ttctgcgcta tttgctggca aaatcgttgt
      301 gaccatgatg tatcctaatg ccaatttgtc ttcaatcaaa attccgacac tgatacctac
      361 tgcgtaacct aacgcataga caaataaatt cagtggatta tctaaacgat ctaaaacaag
      421 ggataagccg acgatataaa tcgtgatctc gaccatactg acaagtggag caatcagtcg
      481 atagcctttc attgtcaaca tgaaacgtaa cgtgttcaga gtgatataag caaaattaat
      541 gaggaagatc atccccaaca tttttggatc aatcatttgt aaaactcctt tcatttatat
      601 aacaacaata tcaaaaaaaa tcgtaagtga agaaaaattg aaactttata catacaaaaa
      661 aatgataaaa tgattataat agaattataa agataggtta ggtgattaaa atgaaaacgg
      721 tcaatcgtgt gttttgtgcc agccacgtgc taagtctcct tctcagtata tacttaggtg
      781 tctattcgat tcaacagcca attgaggtag agaacattgt ttttcagatg tcgctgacag
      841 atggtttaat tttaagcgtt tttttctttt tgatactgtt tattggtaat attttcggta
      901 gcgttgccag ttttttgaat ttttctattt atccactcct ttcgctagca ttaggagtgg
      961 tgggactttg ttctctgtgc ttatttcccg agccattcag tcctgcattt atattatttg
     1021 gtacactgaa cctgttccag acaactgttg gagcatggtt gctatggcga tcaggaaatt
     1081 taatgaaaat aggtgagtaa caattgaatc caattatatt gaaacgttct gtccatcata
     1141 ttccagtgtt agaagttgtt ctgaaagaat taagagagga acgtattccc gttgtcatct
     1201 attatcacgg atggcagaca agtaaggagt tagttttgac gcaaggaaga aaaattgcgc
     1261 agcagggcat tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc aaacagccgg
     1321 tttcttctat tccatcctat acattttggg atagtatcta cggaaatctg tttgaatttg
     1381 acacacttat caagcattta gaaaaaagag gtcttctaca tgatcagctt gcagttggcg
     1441 gggtttcgat ggggggaatg acaacatgcg ccttgttggc taaacatcct catatagtag
     1501 cgggcgtgtg tttgatgggt tcccctgcac ctttggaata tggaaaagaa atcgtaaggc
     1561 gcgcatcgga atataattat gtcgtaccaa aagactattt tgatttgatt tcatgggtta
     1621 agagctatga tttgtcattg cagccggaaa agttagctgg acgtcctctg ttcttttggc
     1681 atggacaagc tgacgaaaaa atcccttata tacaaacgga acgatttgta gaaaaaaatc
     1741 ggcataagtc ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag cgacatatgg
     1801 tacaaatacc actaatggaa gaagcttcca cttttttagc aaaaaatttg ataaaaaagt
     1861 ataaataatt gaaacggaaa atcactcatg acgatagagg attttccgtt ttttattttg
     1921 ggctataata aactctgtta ggaaaatttg gtataattga tagtaagaat gacaacatgt
     1981 tagaaaggaa gagagcatgc ccactttagc agatgttgca aaacgtgcca atgtttcaaa
     2041 aatgacggtc tctcgagtca tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt
     2101 agttttctca gcaatggctg aactggatta ccgtccaaat attgctgcaa aagcacttgt
     2161 cacgaatcgg tcgcagatca tcaaattatt tatactggaa gaaatcgaca caactgagcc
     2221 ctattacatg aatttgctga tgggaatcgc taaacggatc ggtaaagcgc attattctct
     2281 ccaattagtt acaaatgatg ccttcgacgt cggttcttgt gatggataca tcatcacagg
     2341 tgtcagaaaa aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg
     2401 ggaaaattct gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc
     2461 aacgcaatat gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga
     2521 cgagcctttt gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct
     2581 tcctagtaaa atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga
     2641 aaactgggca gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact
     2701 aggagcagta agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat
     2761 tggatttgat ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca
     2821 agatattata cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa
     2881 aggggcgagt caaggttatc gtcgtttttt tcctgaacta gttatcagag agacaacgaa
     2941 ttaagtctta ttactatata tatatttatg aaattcatcg ctattttctt aaaataatca
     3001 gcgcattgct actgaaaaca atgaatctgt gctattattg agaaaagttc catttttgag
     3061 aaggatggtt tgaatgaaag aaatcagacg aaaacatttc cgttttcctg cgtacgatga
     3121 tgagttaggc gtaaaattaa ccaacgacaa gaagcgagta ttatttgacg gacaagaaga
     3181 tcttttgact gaacagaatc agtccccgtt tgaacagatg aaagaatttc atttcacgaa
     3241 cacgaacacc gagaaaatcc gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc
     3301 gcgtaaggaa aatttggaaa agcataaagg aaaattaccg gattataccg gccattacca
     3361 atcagaaatc acatcgacag gacgaaaaaa actttttggt gattcctctc gttctgtcag
     3421 acaaagtgaa acaaggacag agtccaagca tactacacga aacgcatctg ttgacacgaa
     3481 ggtgcgggag cgttcttatt ttgttcctaa atatgtaccc gcatcgatta tcccagaccc
     3541 tagagaaagc aaaatttctg aggatcattt aatcgaatcg atgaaaaaaa cggatgactc
     3601 ttatctgttg ttcgatacag aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc
     3661 tgtacgaaca ttcaatcgaa caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa
     3721 atcgaaaaaa agccatggta tcttagaacg ctcattacaa ggattgatcg atgatcaagg
     3781 aaactctttg ggagaaaata gctattttaa ataagtgata tacaaattta tattcggcta
     3841 cagttggaaa ggaaacgctg cggcgtttcc ttattttata ctctatccga aagtttatat
     3901 cgtatcctca aatttttttg agtttaagcg aattttttct cccaataagt aagaaaataa
     3961 tggcaaaata aaaaaacaaa ctattttcgc agatcgtttg caaaaatagt t
//
LOCUS       DAZKAS010000185         3945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000185 DAZKAS010000000
VERSION     DAZKAS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.189"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            706..1656
                     /locus_tag="ACG8H7_002618"
     CDS             706..1656
                     /locus_tag="ACG8H7_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP8984669.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1677..2210
                     /locus_tag="ACG8H7_002619"
     CDS             1677..2210
                     /locus_tag="ACG8H7_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP8984670.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            2226..2396
                     /locus_tag="ACG8H7_002620"
     CDS             2226..2396
                     /locus_tag="ACG8H7_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984671.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            2846..3379
                     /locus_tag="ACG8H7_002621"
     CDS             2846..3379
                     /locus_tag="ACG8H7_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984672.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            3391..3594
                     /locus_tag="ACG8H7_002622"
     CDS             3391..3594
                     /locus_tag="ACG8H7_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984673.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
BASE COUNT         1415 a          646 c          731 g         1153 t
ORIGIN      
        1 aagaaggggg caaatgaacc gttcaaacat tagatacacc tccttccagt cgcaagactg
       61 tcggataggc gacaagttat tataccttac aaaataaaat ctgtgtggtg tttttaagta
      121 tcccttattt tcgggttgtt agggcttgcc ctgaccgcct gtaaggcgct ttaagaacaa
      181 gataaggttt tttacccaag actgtcttaa aacgacttag acgagctatc agaggcttcc
      241 cccacctccg ttactgactt gtttttagca aaggagcctt taggcgactg cgcagtacaa
      301 tgtgagagca tagcgatcac acttttttag cgaccgtagg gagcaaggtt ttgaattggc
      361 agttttgaaa aatgcgcgaa cctgttggta gaaaaaagaa gtggttttta ggcgggaaat
      421 tttgtcaccc tcttttataa tctcttttta aacctttttt taaacctctt taccgtcggc
      481 tgtgtcctta ctccttcaat ggatacagcg ttttataaac tacattttgt ctgcttttaa
      541 actacatttt gtctgctttt aaactacatt ttgtctgctt ttaaactaca ttttgtctgc
      601 ttttaaactc tttttttgca ctcacgtatg ttataatatg cattatcaca gacaaaacgt
      661 tgtttataac gtctgtatcc attgaaggag taaggaggaa tatctttggc aaatgaaatc
      721 gtgaaatacc atcatgaatt aaataccatt ccgttgcgga aatttacacc agttgaaatg
      781 aatctatttt tctctattgt gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt
      841 acttttgacc aactaaaaga tttaagtaat tataaagcaa ctgctaataa tcgtttcatt
      901 aatgatttag aaatgactta tgataaatta atggatttac gttttggtaa aagaagtgct
      961 aatgggctcg atatagatag attcgttatg tttagtgagt ttaaaatacg tggaactgct
     1021 aaagatccat atgtagacat acaagtttac gaaaaagctt tacctttact aaacaattta
     1081 gatgagtggg ttcgctattc actccaacaa tttaacgaat tacaaagtag ctatgcaaag
     1141 actatgtttc gattactaaa acaatatcga actaaaggtt ttgcttattt ttctaaagag
     1201 gattttcatg aactattaga tattccaaaa agttactgga acaaccaagc aaacgttgat
     1261 aatcgtgtta taaagcctat acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc
     1321 aaaaagaaat acggaaaagg gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa
     1381 ccagaaatca aaaaagcaga tgattttaat aaacatgcac caaccaaaga agaacaaaat
     1441 aaagcattgg ctttagctga taaacaaaaa gaatacgaag aacctgctac aacaattaac
     1501 gaaaaaatca aacagaagaa acgagaactt gaactcctcc aacaaatcaa acaactagaa
     1561 aaagagaaag aagctcttga aaaacaaaca gagtccgcac tttcgaaaaa agagaaaaaa
     1621 agtttattat ctgatttaga taatttattc cagtagcaac ctaggaggca accagtatgc
     1681 aaccacaaga aacctttaca ataaaggaat tgtcagaact ttttaagatg agtaggcaag
     1741 caatatcgaa acatatccaa aaattagatt cttccatgat agctaaaaat gagcgtggtt
     1801 ataaggttgt attgcgttct ggagtattgc aacttgctcg caacttaggc aaccaaatgt
     1861 tgctagatag gttgcacgag tacgaacaag acaacgataa aaatgttaat ataccaagca
     1921 aaaacgtggt tacaaacaag ttgctagatc agttgcgtga agaaaatgaa tttttacgag
     1981 aacaacttgc cctcagagag caaaataatt ctcatataca aaaactactc gaccagcaac
     2041 aacaactcac cctacaagcc aacaaacaaa tcgaaaaact gcaagaaaaa ctacagttga
     2101 cttatacaga agaagctcca gagaatgact caacgactct ttctgaagca acagaaacaa
     2161 tagacaaaca aattgatact gaaaaaaaat ggtggcaact ttggaaataa ctggagggaa
     2221 cattcatgac aagaaaaaat cttctattcg gttctatttt actaggaatt ctaggattca
     2281 gtccgatgtt attgtcagtg atcaataaag ggaataaaca tggtgaattg aaattgaatc
     2341 caaattatta caacagtcct aacgaagaat tgtttgtaaa agaaacagat aaataataaa
     2401 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
     2461 acgcgcttat gaagcgctac caaaaattta tcatatttta ttaaaaataa caacccagta
     2521 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caaccgcaga aaatcaagct
     2581 taaaaagagc ttttgaccta gaccaatcga aaatcacaaa gtgagcgcct gtatctattg
     2641 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
     2701 tcctaatgca ttctaactat accacattat caaaaatata aattatcttt actaaataaa
     2761 atattgacaa gcctatacct tttgttttat gattttcaca aagaaaacgt tttctatata
     2821 tttttctgta tcaggaggaa attctatgaa aaaatttact ttaggggtag ctttgttaac
     2881 tattggggca gttgttttac cgtcaactac tatagtttat gcttcagaaa attcccaagt
     2941 ggtatcatta aacgaaaaca atgctactga aaatgtagtt attgagtaca ctgaagatgg
     3001 tatggtcgta actaaatatg aggatggtta tagtgcggaa gattttgcaa attcttcaga
     3061 tatcatgaca agagctgcag ttgcaagatg gggcaaaaag gaatatacaa atattgctgt
     3121 aaccactgga gtagctgcta atgctattaa tgctgccttt tgggcaggag ggaaagctag
     3181 cacttctatg tttggtatcc caggttgggc aattggtgct ttgttaacag gtgcaagttg
     3241 gactaaaaaa ggtgcttctc cagggaatgc agttgcaaaa gaatgggaca aaaatggtaa
     3301 tggctggatt ggattttatt accaaagggg atatgatgca gctggaagag ttgtagctac
     3361 tagatattca acgttataag ttaaggagtg atgtttttga tatctaaatc attaaaatca
     3421 ttaaaaacat ttactaaaaa gaatccgcaa atagctacta ttcgtatttt tatatttgct
     3481 atagctgttg ctataattgt taatatgcta agcggagggc aattatacaa atctggttta
     3541 tttttagtgt taatcttagt tgtcttagac ataataaagt ctaaaaaaaa ataaatcgat
     3601 acattttaga agaatagggt tgtttgaata ttacaaagca acaatttcac cttcggtgaa
     3661 ataagcgaaa ctttacaaaa agtggctagc aaatgctagc ctttttttgt attgtggagc
     3721 gtggattttg agggggattc aagggggagc ttgcgctcct ttgacaagcg gttatttgca
     3781 ccaaatacgg aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt
     3841 tactcattgt acctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact
     3901 gaaagggtgg gacttatgag cgaacaacat caaaatttgg ctagc
//
LOCUS       DAZKAS010000186         3901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000186 DAZKAS010000000
VERSION     DAZKAS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.190"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..752
                     /locus_tag="ACG8H7_002623"
     CDS             66..752
                     /locus_tag="ACG8H7_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFP8984674.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            754..1698
                     /locus_tag="ACG8H7_002624"
     CDS             754..1698
                     /locus_tag="ACG8H7_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFP8984675.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1897..2541
                     /gene="pcp"
                     /locus_tag="ACG8H7_002625"
     CDS             1897..2541
                     /gene="pcp"
                     /locus_tag="ACG8H7_002625"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFP8984676.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2620..3897
                     /locus_tag="ACG8H7_002626"
     CDS             2620..3897
                     /locus_tag="ACG8H7_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984677.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1245 a          676 c          861 g         1119 t
ORIGIN      
        1 actatatttt ttatgtgttt tgctttataa ttaaaaaaat attgtcattt tatggaggaa
       61 aatatatgga aaattatctt agccttttag gcgtcttgat tgttattctc gggtttgcat
      121 ttaaattaga ctcgatattg atcgtaatgg ttgctttagt tgttacagct ttgactggcg
      181 gtcttggcat tcaaggaatg ttagaacttt taggtacaag ttttgtaaac aatcgcggga
      241 tggcgatttt tatcatcatt atgttggcaa caggaacgct tgaaagaaat ggtctgaaag
      301 aatcagcggc ttccttgatc aagcgattca agaaagtatc tgctggtatg attgtagata
      361 tttatgggat ctttcgaatg atctttgcgg cttttaacgt gagttttggt ggagttgctg
      421 gatttgttcg tccaatcatt ttaccgatgg cattggggac catcgaatca aaagggttag
      481 aagtccatcc taagcatgaa gaagaactaa aaggtatggc ttcagctatg gaaaatgtct
      541 gctggttctt tggccaagta ctattcatcg gaggagcagg ggcgttgcta gtccaatcaa
      601 cactgaaaga tctaggatat gacgtaacat tgggacagct tgctttagtg gaagttccag
      661 tggctatcac ggctttagtt gtggcaagtg tctacttcta tcttaaagaa aagaaattag
      721 cgaagaaata ttatccagga aaggggcaat aagatggact tttttacaaa tcctgatatt
      781 ttattaggcg ataaattact ggagatcctt tatatcgtga tgggactcgt attgatttac
      841 actggagtaa gaaatttaac agataaaacc aatcctcatc gttatgggtc agcctttttc
      901 tggacggtct taggggtagt tattgcagga ggaagatggc ttccggcaat cgtgaacggt
      961 ggcttgatct ttgcgatgac cataccagca attgctaaga aagtcagcaa aggagaaagc
     1021 cggcttccat caaaagaata tatggaaaaa atgtcagaca aactaggaat gaaaattttt
     1081 attccagctt tgagtatcgg tgtctttgcg atcttgtttg ctctatttac caatttaggt
     1141 gctttagtcg gcgtgggcgt cggtgttttt gcagccatgc taatcttgat gttcttttct
     1201 cgagataatc gaccaagtac atttttagat gatgcagcag atatgctggg cacagtaggg
     1261 ccgttaagca tgttgccaat gcttttagct tctctaggtg cggtatttac aagcgctgga
     1321 gttgggacag tgatttccaa tgcagtaggt acgatcgttc cagaaggaaa tgtgaacatc
     1381 ggcatcatca tttacgctct tggcatggtg atatttacgg taatcatggg gaatgcattt
     1441 gcggccatca ccgtgatgac ggttgggatc ggtgcaccat tcgtatttag tcatggagcg
     1501 aatccagcgt taataggaat ggttgcattg acttgcggtt tttgtgggac cctgctgaca
     1561 ccgatggctg ctaacttcaa tatcgtgcct gtagcgatgc tggagatgaa agacaaatac
     1621 ggagtgatca aaaaccagtt gtttattgcg ttatttatgc tagttttcca aattgcttat
     1681 atgattctat ttaaataata gaagagaggg gttgtgacag aataattgtc gcaacctctt
     1741 ctaacattgt agtggaacac tattttttta taagaatata ttaagtttga tctgttatgt
     1801 tatttaaact attgtttcct ttatttacgc tgttcaagga aaatggtatg ataaaagata
     1861 accatatgtt aaatcaatcg gacggaggaa caacagatga aagtactagt aacaggtttt
     1921 gatccatttg gtggggacaa agtgaatcca gcctatgaag cagtaaaaaa aatgccagat
     1981 gaaatcagcg gtgctgaaat catcaaagtg gaagtaccga ccgtattcga aaaaagcagt
     2041 caagtagtca aagaagccat ccaacagcac caaccagatg tcgtgatctg tgtcggacaa
     2101 gcaggtgggc ggtcagcagt ctcgttcgaa cgtgtcgcaa tcaatctagc agaagcaaga
     2161 atccctgata atgaaggaaa tcagccattc gatacggctt tagaagaaaa cggtccagcc
     2221 gcttacttta cctcattgcc aatcaaagca atgacaaaaa atgtacaaga tcatggtcta
     2281 ccagcgtaca tctcttatac agcaggaaca tttgtctgca atgatatcat gtatcgtttg
     2341 cttcatttga tcgaaacaca attcccggca attcgaggtg ggttcatcca cgtgccattc
     2401 tcacccgatc aagtgatcga tcgtccagtg ggtacgcctt ctatgtcttt agaagacatt
     2461 gcttcttctt tgacttatgc agttgaagca gcagtagaaa ataaagaaga tatttccgga
     2521 aatgccggaa caactcacta atttagcaag aaacactggc tggttgctaa cgttatgtta
     2581 gtagccagct ttttgtaatt agaaaggaaa gaaacagccg tggaagaatg gcaatccgtc
     2641 tttgaagaat ggtttccaaa agaaatcagc aaaagctatc ccatcaaaat ttctaaacaa
     2701 tacacaagca gtcaacgttg ggagatctat gcaaaattaa cgaaaaaaca gcgggaacta
     2761 gtcgataagc accgccgata tctaatcagc tctcgtttta tggaagaaca ttatttagct
     2821 gcaacagatt gggtgttttc tgacttcaag atcaatccgt tttttcgaac aaaacgaagc
     2881 cagcaaaaat tgtattgtga gtgtggtaga gaattaaaag tgcaatacat cgttaaatcg
     2941 ccaaaaaccg gaaagatcct aaaactaggc atcaaccact ttgccgatca cctgcatgtc
     3001 tctccgacag tggcagcttc gatccatcag ggaatgacaa aagttgatct ggccttagac
     3061 gaattattat ggcttaagca gaagaatatc gattttcctg aaggactatg gcaaaaatac
     3121 tgttttgtct tgtaccaaaa tcgtcggatg aaacagccat atttaccgga tataaaatta
     3181 gctcagcggc tcgcagaatt tcgccaagta gaaatgccga tttacatagc ggattatcaa
     3241 gcattggaaa atgaaatcaa gaaaataagt gaacacatca atgggcagtc aaaaaagcga
     3301 cagataaaaa aagaactatt tgacgacttt gcagaagaat tagtaaagga tgtagaagaa
     3361 ttcttgatca actatcgcgc atttttacgt aaagattggc aatccatcgt atatgaagaa
     3421 gtgccagtac atcctaatgc ttattttgaa acatttattt ccgtgttgag aaagacaaaa
     3481 cgtcaacgaa caccagaagt cactgctcag atggaatatt ttgcgaaaaa ccaacgattc
     3541 atccaaccaa aaatctatct atttatttgg aagcaatatt gtcgctatgg ttttacagaa
     3601 gggttcttcg acagcattcc tcggatagtc agaaatggtt ttttaaaagt attgcgtaaa
     3661 gaacgagaag ctatacaatc tgctgataag aaagaccgta cagtctcaaa agaaaaatgg
     3721 caactagtag tgaaagatat ccaatctgga aatgtgcaag agacaatcga caaatggaaa
     3781 ggaaaacatt atcgttttac tgaagctcag aaacaagcgt tggaatatta tcagaagtta
     3841 gaagaatcac tgcgtttcaa tgatgaggca agaaagtatc tgaaggaatt actataaaaa
     3901 a
//
LOCUS       DAZKAS010000187         3898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000187 DAZKAS010000000
VERSION     DAZKAS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.191"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(657..1160)
                     /locus_tag="ACG8H7_002627"
     CDS             complement(657..1160)
                     /locus_tag="ACG8H7_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFP8984678.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1519..2544
                     /locus_tag="ACG8H7_002628"
     CDS             1519..2544
                     /locus_tag="ACG8H7_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP8984679.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2867..3745
                     /locus_tag="ACG8H7_002629"
     CDS             2867..3745
                     /locus_tag="ACG8H7_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFP8984680.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1301 a          605 c          727 g         1265 t
ORIGIN      
        1 taagagataa aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga
       61 tatacaacag aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt
      121 ttggaataaa aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata
      181 acgacacaga aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt
      241 tcctttgaaa tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga
      301 agggagccta aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag
      361 ctttttatag aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta
      421 gattgagata ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc
      481 ctttttgtta aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc
      541 aataatatag tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag
      601 accgtcatag atacacattg gaatgtgtct attacggtct ttttttacga acgattttat
      661 tgaagacgga cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg
      721 tatcccaaaa taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt
      781 tgataggccc ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc
      841 gctacgccaa taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata
      901 atccctaatt ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa
      961 ggaaaaaaga caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa
     1021 aaagtagaaa aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga
     1081 ttaaatgata gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa
     1141 aattttgata cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg
     1201 ctgaaaaatc ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta
     1261 gaagcttttt tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg
     1321 gaaaagttgc tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt
     1381 tctccaagtt gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata
     1441 ctaggtaatg ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta
     1501 ctaggaagga gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat
     1561 ccttttcgta tcatgaatct gatgctactt atctttattg tattatggtt aggttacata
     1621 aatcatgatt ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat
     1681 caaaacatac cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg
     1741 gttgctttcg ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta
     1801 gctttggttg ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt
     1861 ggcttgtttt ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa
     1921 ttaccgatcg tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg
     1981 attacaaaat tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa
     2041 agatttcacg aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt
     2101 ctttccgttt atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca
     2161 tgtgcatcga ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat
     2221 ctgattggat cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg
     2281 cagttgcaga ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct
     2341 agaaattatg cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt
     2401 attcgtggag catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga
     2461 agtttcatcg gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg
     2521 gttgttcgtc aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa
     2581 gatttttgtc acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca
     2641 aattcacaca aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa
     2701 aattttgttt atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa
     2761 agtcttacag atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa
     2821 agtatgttta taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga
     2881 tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac
     2941 tttaactctt cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga
     3001 tgaggtgttt gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc
     3061 aaagaaaata gacaaagtct atgaagagga accttttgca gcattagcta tttatctata
     3121 tgcagcttct caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta
     3181 tttggcaaca atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag
     3241 ctatcgcttt gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat
     3301 tggtgaattg gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga
     3361 aggaaagcag tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat
     3421 aagagatagt tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta
     3481 ccaagtgatg tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa
     3541 agagtcaaaa atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga
     3601 cgatagtgga gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga
     3661 aagtacgagt ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc
     3721 tggcttaggt attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag
     3781 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
     3841 gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaa
//
LOCUS       DAZKAS010000188         3877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000188 DAZKAS010000000
VERSION     DAZKAS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.192"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..915
                     /locus_tag="ACG8H7_002630"
     CDS             25..915
                     /locus_tag="ACG8H7_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP8984681.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(988..1812)
                     /gene="nadE"
                     /locus_tag="ACG8H7_002631"
     CDS             complement(988..1812)
                     /gene="nadE"
                     /locus_tag="ACG8H7_002631"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP8984682.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1824..3296)
                     /locus_tag="ACG8H7_002632"
     CDS             complement(1824..3296)
                     /locus_tag="ACG8H7_002632"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP8984683.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1135 a          795 c          684 g         1263 t
ORIGIN      
        1 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
       61 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
      121 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
      181 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
      241 tcgagccttc gtgtcgataa aatcgatatt agttttccag aaaaaatatt caatgctttt
      301 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
      361 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
      421 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
      481 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
      541 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
      601 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
      661 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
      721 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
      781 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
      841 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
      901 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
      961 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
     1021 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
     1081 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
     1141 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
     1201 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
     1261 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
     1321 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
     1381 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
     1441 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
     1501 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
     1561 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
     1621 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
     1681 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
     1741 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
     1801 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
     1861 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
     1921 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
     1981 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
     2041 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
     2101 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
     2161 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
     2221 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
     2281 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
     2341 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
     2401 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
     2461 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
     2521 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
     2581 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
     2641 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
     2701 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
     2761 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
     2821 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
     2881 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
     2941 ccttccactt ggatcaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
     3001 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
     3061 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
     3121 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
     3181 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
     3241 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
     3301 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga
     3361 aaagaaatct tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa
     3421 tgaactccat ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat
     3481 acttatttta ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact
     3541 tgataaaata agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac
     3601 aaatataacc aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac
     3661 gtctttcctt ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat
     3721 ttttttggaa aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa
     3781 atatcacacg tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca
     3841 aaaatggcga gacatcaatc agtctcgcct tgttttt
//
LOCUS       DAZKAS010000189         3876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000189 DAZKAS010000000
VERSION     DAZKAS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.193"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACG8H7_002633"
     CDS             158..2257
                     /locus_tag="ACG8H7_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFP8984684.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACG8H7_002634"
     CDS             2453..3316
                     /locus_tag="ACG8H7_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFP8984685.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="ACG8H7_002635"
     CDS             3421..>3876
                     /locus_tag="ACG8H7_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP8984686.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAZKAS010000190         3770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000190 DAZKAS010000000
VERSION     DAZKAS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.194"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..1023
                     /locus_tag="ACG8H7_002636"
     CDS             55..1023
                     /locus_tag="ACG8H7_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8984687.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1091..1849
                     /locus_tag="ACG8H7_002637"
     CDS             1091..1849
                     /locus_tag="ACG8H7_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP8984688.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1917..2627
                     /locus_tag="ACG8H7_002638"
     CDS             1917..2627
                     /locus_tag="ACG8H7_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP8984689.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2663..>3770
                     /locus_tag="ACG8H7_002639"
     CDS             2663..>3770
                     /locus_tag="ACG8H7_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP8984690.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAY"
BASE COUNT         1247 a          554 c          845 g         1124 t
ORIGIN      
        1 tacttttatt ttggataaaa ttaacatttt taaaatttta aggaggaaat gttgatggat
       61 gtagcaaaaa cggttaacgc aattgtagaa aaaaatgatg gagagatttc tggaatttat
      121 ttggtggctt gtggcggttc gctggtagat atgtatgtct cagattattt tcttaaagcc
      181 gaagcatcga agtgtattac aggcttatat acagcgaacg aatttaacca tgtaacccca
      241 aagcaattag atgataaatc tgttgtcatt ctatgttctc atggaggaaa tacagcagaa
      301 acggtaaaag caggagaagt cgctcaagct agaggagctg tgactgttgg tctgactcat
      361 aataaaactg cggatttatt aaaggtttct gatcatagtt tcctttacga atggggagat
      421 gatgcaaaaa tagtcaataa tcctatggcc atcattcttg atttaactgt atctttggtc
      481 aatgttaccg aaacatttga acattacggt aaattcagag aaggcatgaa ggttatcgat
      541 accgtaattg aaaatggcat taagcaggtg caggatcgta ttaaagtgtt tgctgataag
      601 tacaaagatg aaacaatgtt ttatatctta ggtagtggac catcatatgg acatgcttat
      661 ggtttttcaa tttgctcttt gatggaaatg caatggcttg attcaacttc tgttcattct
      721 ggagaattct tccatggacc ttttgaagta acagacaaag atacgaattt tattctcttg
      781 atgagcattg gacgcactcg tgctttagat gaacgggcaa aacgattcct tgataaatat
      841 gcagaaagag tagaaatcgt tgatgcaaag gaactaggat tggatatgat atcagatgaa
      901 gtttctgaat tcttcaatcc cattttattc tacagtattc tttcagaata tcgttcagct
      961 ttagcagatg tacgcaatca tgatttagat gttcgtcgct atatggggaa agtggagtac
     1021 taggaggatt aaaatgaagg tattaggctt aggtgataac gtcgtcgata aatatgaaaa
     1081 tctgggaatt atgtatcctg gaggaaattc attgaatttt gcagtttttg caaaaaaact
     1141 gggacatgaa agtagcttta tgggagtttt tggctcagat gaggaggcag ctcatgtttt
     1201 atcagcaata aaagaaatag gactggacaa cagtcactct cgctttgaaa aaggcgagaa
     1261 tggctgtgcc agagtgaaga ttgatgaggg cgaccggatt ttccttggca gcaattcggg
     1321 aggtgttact cgtgaatacc cgattcagct tactgaagaa gacagagaat atttaaacca
     1381 gtttgaattg attcatatgg gattatatag ccatgtcaat catctattag aagagttaca
     1441 aaatgtttct ggaaagattt cttatgattt ttctgacgac tttacggaag aagaggttca
     1501 gcgggcaatt gataaagttg attttggctt cttttctata gaagactttt ccgatgaaga
     1561 agtaaagcag tttttgatga agcatttttg tgagcgaaat gatgtattga ttgcaacccg
     1621 aggagaaaaa acggctattg gttatgatgg agcaaagttt tatgaagtga accccgtgct
     1681 agaagaacca gttgatacaa tggcagcggg agattctttc ttaacagctt tcctacttta
     1741 ttttctcgaa ggggacgata tagtcaccgc gatgaaaaag gggaatgaat ttgctgggaa
     1801 gtcttgtctg gttgacggtt cgttcggctt tggtattcat tacgattgag cttgttttaa
     1861 attcctgcat ttgataacct aaagaaagaa atcccggtaa agtggaggtt aaaaaaatgc
     1921 tggatacaaa tgcacaatta ccattgtact tacaattaaa agaaatatta aaaaataaaa
     1981 tcaatacagg ggagtatccc gagaatacga aaattcccgc tgaatcagag ctgatagaaa
     2041 gttatgaggt cagccgaatc acagtgagaa aagcagtaga agaactggta gaagaagggt
     2101 acctacttaa aaaacaaggg aaaggaactt ttgtcaaccg gcataaagtt tttcgtaaaa
     2161 ttgaatatat tatgggtttc agtgagagtt gtaaatctaa tggttttaca ccgactagca
     2221 ttgttttgga acgcaagatc attccagcaa ctgaagggat ggcaaaaaaa ctcgatatcg
     2281 ctcaaggtga tgatgtttta tatatccaga gaaagcgaat ggcggataac atccccatta
     2341 tgttagaaaa caattatttt tcttatgaaa gatatagttt cttaatggag gaagagctga
     2401 ctgattcttt gtatgaagta cttaaagtcc aaggtacctt agccatcaat cctggggaaa
     2461 ctgcacttga aattgtcaca gctgatgacc aaattgccaa aataatggaa gtagctgttg
     2521 ggacgccctt tttctttatg gatactttaa tcaacgatca aaacaacata ccaattcatg
     2581 taggtaatca atattacctg ggagaagtat atcgttttta cttatgattt cctgtttgga
     2641 tttcaaggag tagattttat ttatgaagca aatattgcgg tcaaaaatga tttttgatgg
     2701 agagcagcca cctttcaaag gttatattgt aatcaatgaa gggatcattg aaaaggtgga
     2761 aaacaactgg aattacagtg atctattaaa tgaaaacacg gaattaattg cttatgatga
     2821 tcaattcgtt atgccagcaa ttcatgataa tcatgtattt ttcagtggtt atttggctat
     2881 gaatgctggc atagatttaa gcgatacatc ttcagctaac gaggctgtag agcgtattaa
     2941 agctgtaatg aaccataaaa aagcagatga accaatttat gctcatggct gggatgctgc
     3001 cctgtggaaa actgacccat cagaagagct attgaatgaa ataaaccact ttggtccaat
     3061 cacagcgatc gacaaaaatc gcagccattg ttggatgaat gctattgcta agcagactta
     3121 tggttttaca gaaaaagaaa ccagtgctga aagtcgagta ttattaatta aggaaatgct
     3181 agcaaacaaa aagcttttag cagaggttta cgctgaattt gaagcattac tgctttcaaa
     3241 aggtgttata tcaatgaaag aaatcgtttt tgatgacgca gaatttcttt ctaatatacc
     3301 tcagaggaaa atacttagta acctatatgt gcaggcagtc caagaacctc tacagcatga
     3361 acttctatat gagtataagg aaaaagtttt ccctgaaaag gtccgctttg gtggagtgaa
     3421 gatcatggta gatggtgttg ttgcggatag aagtggagat atttacggtg tttatcagtc
     3481 aggagtagct tcacccgaga ttgactatga ggcaattaaa gcagaagttg ctgcattcaa
     3541 tgaagtagag attccttgct gtttgacaac ggaaggagat aaagcgggtt ataaagctgc
     3601 gtcaattctt gtcgaatttg gaaagcggtt acctgaaggc gtttttaatt cgattagtga
     3661 tttggaaatg gttactcaaa agacagcaga aaaaatgaac gaaggacaag tagtagcaga
     3721 ggtatatcca caaattctcg gcttgaatca aacgttagaa gaggcttaca
//
LOCUS       DAZKAS010000191         3758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000191 DAZKAS010000000
VERSION     DAZKAS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.195"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1912)
                     /gene="uidA"
                     /locus_tag="ACG8H7_002640"
     CDS             complement(104..1912)
                     /gene="uidA"
                     /locus_tag="ACG8H7_002640"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP8984691.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(1940..3706)
                     /locus_tag="ACG8H7_002641"
     CDS             complement(1940..3706)
                     /locus_tag="ACG8H7_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFP8984692.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
BASE COUNT         1152 a          752 c          470 g         1384 t
ORIGIN      
        1 actagaaaaa aatatccttc tttaaacaga aaatcttttt ataaaaaaaa ggagttggca
       61 attaagcagc cactcctttt ttatattcct tataaaaggt aaattatttt ttataactga
      121 aatttggtat tgatgaccat cgtttcttta gatagtgagc aatcatttta ggctttctat
      181 ctctagtaaa gattcctttc ttatttcctt gcactcttaa aatcccttga cttgtttcaa
      241 aatctgcaaa attccacact tgttcaccaa caaaattgtc acatttatct attacttcgt
      301 gatttgcttc gtaataaccc acttgatact cttctgtaaa cattgttggt actgtatcat
      361 gaagtccagc tactgtatct gctccatatt ctgtaaacat tattggcgta tcgggacatc
      421 tttccatcca tccatctagc tcttttttca acagatattt tgcatgtttt aggtctccac
      481 ctgaaacata ccaaccataa tagcgattta aacataaaac atcgacaata tctcctactt
      541 tacaaatatc tggtgttgac tttaagtaag taacgattgt tacaggtctc ttctgagggt
      601 caagttcttt agtcaattca actaaaggtt caaaatattc tttagcgcct tcagaatccg
      661 aatctggttc atttgcaata gaccacatta cgacactcgg atggttcttg tcgcgttcta
      721 tcaattcctt aatcgccatt ttgtgagcat tttctgtccg tatttctttc caagtatcat
      781 gtttttctac gttgtcatct aataaagtag ccatgaaatt caaatgtaat cctactgcag
      841 gtacttcatc aataactaca ataccttctc gatctgctaa gcgcataatt tcttctgaat
      901 aaggataatg tgaagtccta aatgaattag caccgatcca tttcattaaa ttaaaatctt
      961 tgatatttac tgcttcattc aaaccacgac cattaatata cgtgtcctca tgcttcccaa
     1021 atcccttaaa gtaaaaaggt ttattattaa tcaaaaactt cccattcgaa acctgaatac
     1081 tacgaatgcc aaattcttct gtgtacgtat caatcacttt attttcgtta attaaactaa
     1141 ctttcatact atataagtaa gcatttagag gctcccaaag tgttgggttc ttaagttgaa
     1201 tcactccttc tttcccctta gcttctgcta taagttgagc ttcttcgtca tagattgcta
     1261 ttagaatttc tccggtccat tctgtatcga tattaaattc tacatttgca tctgaatcaa
     1321 ttatttttgt attaatagtg acatccttta tatagtcttt cggagttgta tacaatttaa
     1381 cgggtctatt aatgcctgca taattgaaaa agtcaaaatt aaccaaattt tcttttttta
     1441 tgtctccatc ttcctgtatc gtttctttca aaatccctac aggtaaagtg ctatcgtcaa
     1501 taacattgtt tacagccact gtaattctgt tacttccatc ttttataaac tgattaattt
     1561 ctgattcaaa tggcgtaaac cctcctttgt gttgattaat caattttcca ttcaaaaata
     1621 cttttgcttc atgtgttgca gatccaaaac gaagaactaa tctttcttct aataattttt
     1681 taggaagaat cacttctttt tcataccaca cccatccaac atgatttcta atgttctctg
     1741 tttcaaataa atcattataa gaagaaggaa ctgaaattgg ataactctct ttttcaacca
     1801 ttggttctaa agcaagttct tcttgtaagt catcatttgt atctaactta aatctccaaa
     1861 gaccgcttaa atcaattact tgcctcgtct ctgtcataat gggatataac atgtaaatca
     1921 ctcctatata atttaaatat taaataaatg tatcctttaa cccacaataa gcatctattg
     1981 gacttattcc cttaaattca gaaaggccca ttagtttgtc cactaatatt tctaaagttt
     2041 cttgttttcc atcataagta ttgatataag tttttacttg aggcacatca gctaaatgaa
     2101 acggacactg tacagataca aaaattgttg gaatttcatg aatataaaat ggaatatccg
     2161 gagtaccttt acttgctggc caaacaattc tttgaactgt acctgggtta acattagcta
     2221 tattaataat caagtcatat ttttcagtta aatctttgat tggtctctta gcagcatata
     2281 ttccagctat atttttaatt ttttcttctt gcgataatcc tttcaattct tcttcaggtg
     2341 aaatgtattt ttcaacttca aatccttttg ctgacaaaat ctgtttcaat aaatcaatgg
     2401 cctgaggtct atttccagca actacttcac caaacccacc ttttacccca tgtacatcta
     2461 ccagtaaaat ccttttatat ttttctggta caataggcca aatatcttgt ttatctttaa
     2521 ctagtgtaat tgcttgatca gctacctctt taaaaattga tttattttca attaaaccaa
     2581 ttttttccat agcttcttgt ttcggtaaca aaattttatt cttttctttt ttatgcaaac
     2641 ctagtttagc ctttaagcct aaaattcttc ttaatgcatc acgtaaacgc tcttctgaaa
     2701 taattccttt gtaataacct tccatcatat aattaaagtc ttcatctgga tcattaaaaa
     2761 ataggaatag atcacatcca gccgcaatag aagtgggcaa taattctgac cgtttcattg
     2821 ccccagtcat tcctaacata tgactagcat cagtaactac taatccatta aacccgagtt
     2881 ttcctctaag taagtcagtg attaattctt ttgataaagt tgcaggaagt aaatcttcat
     2941 ctttcagatc aagattaaaa tgttttgaat aagctggcat atggatatga cctgccataa
     3001 ttgaaggtaa tccagcttct attaagcctg agtatacttt tccaaaagaa ttatcccatt
     3061 cttctgtact taaactatta atactaaaag atagatgttg atctcgttcg tcaataccgt
     3121 caccagggaa atgtttagct gcaggcaaaa ttccactctc ttgaatacct ttcatataag
     3181 ctaaactaag ttctaaaact ttatcagggc tttgtccaaa agatcgacta gaaatgatag
     3241 gatttctcca atttctatta atatctacaa taggagcaaa actccagtta catccaatag
     3301 ccgatgcttc aattccagaa actctcccta attcataagc atatttcgca tcatttgttg
     3361 caccaatttt tacttctaca ccaacttcgg taccatctgt acaagctccg ttaccacctg
     3421 cttctgtatt agcagcaatt aataaaggaa ttttgctttc tgtttgtagg atatagtttt
     3481 gatcatatac ctcatcagca gttcctggat tatatcgtac agctccaata tggtactgat
     3541 tgaccatact acttaagtaa tttttatcac gactagatcc catattaaca aataattgac
     3601 ctattttttc ttctaaagtc atagcatcaa tcgtctcttc aatccaagaa atagcatctt
     3661 catctaaatg atatggtttt tttcgtaagt ctacttttat agtcattttc tttctcctct
     3721 acttattagt aagatttttc caaaaagtat ctatatat
//
LOCUS       DAZKAS010000192         3718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000192 DAZKAS010000000
VERSION     DAZKAS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.196"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACG8H7_002642"
     CDS             102..2543
                     /locus_tag="ACG8H7_002642"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFP8984693.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="ACG8H7_002643"
     CDS             complement(2873..3577)
                     /locus_tag="ACG8H7_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984694.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1193 a          654 c          813 g         1058 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaa
//
LOCUS       DAZKAS010000193         3717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000193 DAZKAS010000000
VERSION     DAZKAS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.197"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..715)
                     /locus_tag="ACG8H7_002644"
     CDS             complement(131..715)
                     /locus_tag="ACG8H7_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8984695.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(926..1129)
                     /locus_tag="ACG8H7_002645"
     CDS             complement(926..1129)
                     /locus_tag="ACG8H7_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984696.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1139..1561)
                     /locus_tag="ACG8H7_002646"
     CDS             complement(1139..1561)
                     /locus_tag="ACG8H7_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984697.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1799..2413
                     /locus_tag="ACG8H7_002647"
     CDS             1799..2413
                     /locus_tag="ACG8H7_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8984698.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2578..3402)
                     /locus_tag="ACG8H7_002648"
     CDS             complement(2578..3402)
                     /locus_tag="ACG8H7_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984699.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3426..3581)
                     /locus_tag="ACG8H7_002649"
     CDS             complement(3426..3581)
                     /locus_tag="ACG8H7_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984700.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT         1235 a          642 c          526 g         1314 t
ORIGIN      
        1 atattaacaa atgaataaaa tccttacaac atttgagaat aagttatcag tagacactta
       61 caaaaaattc tcatactatt gtcacttttc tagataaatg gcaaaaattt tcagcattaa
      121 atgacaaaaa ctaatgagaa tttgtctttc catttttctc ctccattttt gccaaataac
      181 gataaatggt ggttcttgag acgttccacc gttccgcaat agtcttaata ggtgtaccgc
      241 tatccactaa agtcttcagt aattccaaat cttgttcaga caatttttca ggacgaccac
      301 caaaccgtcc tcttgcacga gcagctgctc gtcctgctgc ggatcgttcc aggatcaaat
      361 tacgctcaaa ttctgcaaat gccgcaaaca aatgaaacat gagctgtcct gtagaacttg
      421 atttgtccat cgtaatattt tcttgaatac tatgaaaact cacaccacgc tcattcagtc
      481 gattgacgat tgaaatcaaa tcttccatat ttctacctaa acgatctaag cgccaaacga
      541 caagtgtatc gccagagcga acaaaatcaa tggccatttc taatcctggt cgattacttt
      601 ttgccccact catatgatca gtaaaaattt tttcacaacc aaatttattt aggctgtctt
      661 cttgtaaatc caaattttgt aaaccagtag atacccgggc gtatcctatt ttcaaaaaaa
      721 tcactccttc attaatttta ttgtaacata actttatcca tatagtttaa ctgaacatag
      781 atattgttat gggtttatga acatgaaatg aattgttttt ttgaaaaata aagcaaaaaa
      841 agaaagtgtc cagaaatggg tgattttatg aacactttca attagaaaaa aagaatgtcg
      901 actagacact ctttttttaa aatcattatt tttctttttg atatgttttt tggtctcttt
      961 tttttctttt ttcttcagaa tcttctagat tagaaattga tttttgaatt ttttgtgtct
     1021 cctgtaaagt tcgctgaact tgtaaagctg attcttcttt cggtaaatta attttactta
     1081 gaatatcgct atcttgataa aaatttggtg tgttcacaaa ttctggcata aaaaatccct
     1141 actttctagt taaggttcct gaataagtcg caatccaatt gtttccagct gatttataac
     1201 tttgtaaata caattttcca tagtaccaat aaccatttct tttttctttc caattccgtg
     1261 attgaggagg tctagaaggc caaccaactt tatcaaaaat catcgttcct gtcactcgta
     1321 tcgttttaga agctgctaga gcaagtaatg gagaaactat taaataattt tcagtaacag
     1381 ttaagtcgtt agttgggtta ccaatggcta ttccgtccct atctaaagga gttaattctt
     1441 ggttattatc tactacagta gttggttcat ttgtactatc agcaaatgta ggaattgtag
     1501 ttccagttac actaattcca atagttaaaa tacctaaaga taaaataaac ttatttttca
     1561 ttgtctatca cctcgaaatc aaaactaaga aaacgttttc ttaataaatc ttaacagata
     1621 aatcaagtta tatactagaa aattcataaa atatattttt aatgtcataa tcgtttaaat
     1681 tttcttgatt gacatttatt tacaaaaaat attgccattt aaaaagaaaa gtgacatatg
     1741 gcttcctcgt gtatgattag ggtgtatttt aaatagttgc agaaagtagg taaaagtttt
     1801 gagagttgca tacatacgtg tctcatcgat cgatcaaaat gaacaacgcc agattgaaga
     1861 aatgaaaaaa ttcggtgcag agagaatttt catcgagaaa caatctggcg caacgattac
     1921 tcatcgacct gttttccaag aagcattaga ttacgtccga gatcaagata tttttattgt
     1981 ggaagctatt gatcgacttg gaagaaatta tgacgagatc atcgcttcag tcaattactt
     2041 gaagaagaaa aatgtgaagc tgatcattac tagtttaccg atcatggctg aagcaattgg
     2101 caatcctctc ctggataaat tcattaagga tctgatcatc caaattttgg ccatgatcgc
     2161 tgaacaagaa cggactgaat cgaaacgtcg ccaggcacaa ggaattaaga tcgcaaaagc
     2221 aaacggcgtc tacaaagggc ggcccaaatt atacagtgca gacgcaaaag atccccaacg
     2281 tcgccttgtt tacaaaagca tcgttgaaga tcttaaaaat ggtgttgcga ttgctaagat
     2341 tgctaaagac tacaatgtta cgagacaaac cgtgtataga ataaaaaaag acagcatggt
     2401 caatgacaaa tagttgatca tgctgctttt attatcttct catctttaat ttcctacata
     2461 taattactat ctataaaatt aagtcttact cagaacagat aaagcataaa attccaacaa
     2521 accatttttt acttaactaa ataaagagtc tgttaatata atcttcactt atttacgtta
     2581 atcccctact gtatttgttg gttccttatc tatattttct acaaaatcct tattatcttg
     2641 tgatttagct tcgtataatt ctttgtatgt cgacacattg gttatactaa ttcctatagc
     2701 tagttcttga gactctactt ttctctctag ctcctttgac aaagtgattt gagatttatt
     2761 tggttccgtt ggagtcaatt ctatagattc ttcataaaaa tctctataca taaagctatc
     2821 attaatttta taaaaaaaat taaatgtttt tagcactgcc atataacatt caaaacattc
     2881 ttctatagtt ggtttacttc cttgaggaga atgaaccaat ttattacgtt tttctatgaa
     2941 atttttaatt tcctttctta tttctttcgg aaactcaata ttaaagtacc ctttaaacat
     3001 gctccctaat aatttgtcta cagggggaga ttgtaaattc tcaaatatta gagttaaatc
     3061 aggtttctct tgttgataaa attcctttac ccctatttca caggctgtaa ttatcgaaat
     3121 aaacctatct ctagtatttg atatattttt acttctttta aggagtataa gagaaaaggg
     3181 gatactttcg tgtccatgca tgtaattaac taattgttga gcttcagaag ttatatctct
     3241 aattatatag ttaacggaat taggaagtgt ctttaactct aatgaaattt ctatccaatt
     3301 actatcttct aatctgtata tgctcgaagt ataatttgat tcgtcagcaa gcatcgctac
     3361 atcccaaaca ctgatcataa aatcttttaa acgttcaatc atttcagcaa tattgctaaa
     3421 atcagctaat ttttctttag aataaaaaaa acatttatat cagaatcaat aaattctatt
     3481 ttttttatta ctatatcgtc atcattatct tgaccattta tgtttattgt tccctgagtg
     3541 gggagtactt ctttcaaatt caagttaaat ttatattcca ttacttctcc tccaatatat
     3601 aaaaattact taactgtatc caaatttaat atgtaaataa atcattaatt tttagctaaa
     3661 ttctatgaga agtttatttt tgttgttttt gctaattaac taaaactagt acaaaaa
//
LOCUS       DAZKAS010000194         3714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000194 DAZKAS010000000
VERSION     DAZKAS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.198"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..545
                     /locus_tag="ACG8H7_002650"
     CDS             75..545
                     /locus_tag="ACG8H7_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFP8984701.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
     gene            complement(465..683)
                     /locus_tag="ACG8H7_002651"
     CDS             complement(465..683)
                     /locus_tag="ACG8H7_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984702.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ACG8H7_002652"
     CDS             790..1104
                     /locus_tag="ACG8H7_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984703.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ACG8H7_002653"
     CDS             1208..2386
                     /locus_tag="ACG8H7_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8984704.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2567..2752)
                     /locus_tag="ACG8H7_002654"
     CDS             complement(2567..2752)
                     /locus_tag="ACG8H7_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984705.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            2789..3082
                     /locus_tag="ACG8H7_002655"
     CDS             2789..3082
                     /locus_tag="ACG8H7_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFP8984706.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3316)
                     /locus_tag="ACG8H7_002656"
                     /pseudo
     CDS             complement(3170..3316)
                     /locus_tag="ACG8H7_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1270 a          549 c          717 g         1178 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaa
//
LOCUS       DAZKAS010000195         3713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000195 DAZKAS010000000
VERSION     DAZKAS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.199"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG8H7_002657"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG8H7_002657"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP8984707.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACG8H7_002658"
     CDS             complement(2261..2512)
                     /locus_tag="ACG8H7_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFP8984708.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACG8H7_002659"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACG8H7_002659"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP8984709.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACG8H7_002660"
     CDS             3462..3680
                     /locus_tag="ACG8H7_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984710.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DAZKAS010000196         3697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000196 DAZKAS010000000
VERSION     DAZKAS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.200"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..486
                     /locus_tag="ACG8H7_002661"
     CDS             <1..486
                     /locus_tag="ACG8H7_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP8984711.1"
                     /translation="LIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLKDESNTTVSD
                     ANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKTGADIDHFDK
                     ALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQETIKKLRIG
                     G"
     gene            493..1344
                     /locus_tag="ACG8H7_002662"
     CDS             493..1344
                     /locus_tag="ACG8H7_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFP8984712.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            1356..1625
                     /locus_tag="ACG8H7_002663"
     CDS             1356..1625
                     /locus_tag="ACG8H7_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984713.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            1630..1794
                     /locus_tag="ACG8H7_002664"
     CDS             1630..1794
                     /locus_tag="ACG8H7_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984714.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            1787..2089
                     /locus_tag="ACG8H7_002665"
     CDS             1787..2089
                     /locus_tag="ACG8H7_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984715.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            2086..2397
                     /locus_tag="ACG8H7_002666"
     CDS             2086..2397
                     /locus_tag="ACG8H7_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFP8984716.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            2394..2612
                     /locus_tag="ACG8H7_002667"
     CDS             2394..2612
                     /locus_tag="ACG8H7_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFP8984717.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            2623..3438
                     /locus_tag="ACG8H7_002668"
     CDS             2623..3438
                     /locus_tag="ACG8H7_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFP8984718.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            3440..3565
                     /locus_tag="ACG8H7_002669"
     CDS             3440..3565
                     /locus_tag="ACG8H7_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984719.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
BASE COUNT         1407 a          565 c          821 g          904 t
ORIGIN      
        1 ctcataggcg atattttcat cgatacaggc atgccaacgg acttctggaa ggaattcttt
       61 tacttccgct ttgaaggtgt cacagggcgc aaaataagcc tcaaagatga atcgaatacg
      121 actgtgagtg atgctaacgt cttagcaaat atcatcttag atttcatctt tgaacatcat
      181 attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta cttctacaaa
      241 tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag gagctgatat cgatcacttt
      301 gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc attcagagta cacatttgca
      361 gcactctgca gaatccatca cacggagaaa cacaagatag gtgtgatcaa tttcaaaaat
      421 aaatatcaaa tcaaagggat caagttaaac caggaaacaa ttaagaaact tagaatagga
      481 ggataaaaaa tattggctga caacaaacga tactactatt taaaactaaa agagaacttt
      541 ttcgatagcg acgagatggt tcttttagaa agtatgccag atggatacat ttattctaat
      601 attcttctca aactttattt aagaagtctg aaacatgaag gcaaactaat gtttaatgac
      661 aggattccat tcaattctac aatgcttgcg actattacaa gacactctgt gggagttgta
      721 gaaaaagcag tacaaatatt tcgtgattta caacttattg aggtattaga taacggagca
      781 atttatatgt ctgatataca aagttttatt gggaaatctt caactgaagc tgataggaag
      841 agaaaataca gaaaagaaat tgaggaagca aaacagaatt taataacaag tggacaaatg
      901 tcggacaaat gtccggacaa aactacacca gagttagaga tagagttaga gaaagatata
      961 gagttagaga aagatataga gaatgtaacg ccttcgaaaa aatcgaaggc taagcccgtc
     1021 cgtcataaat acggagagta taaaaatgtt cttttgtcag atgagcagat ggagaaactc
     1081 aaaatagaat tccctaatga ctatcaagaa cgaatagaac ggctatctga gtattgtgaa
     1141 tcatctggta agacttataa aaactatttg gcaactattc gaagttgggc aaggaaagaa
     1201 aaaagtgagc ctaagaatgc aagtggtgca tacaagcgca caggaagacg agagaagctt
     1261 cctgaatggg caatcgacca agaagcctat ctcaagaaaa aagcgctaga acgagctaat
     1321 agacaatcaa aagcaccatt ttaagaggtg gaaaattgaa aatcgattat ctagaactaa
     1381 ttaatgaaat agcaaagtat aaaactggtg aggaaataga aatcctgaga gacgtatatg
     1441 atcaactcga agaagctgga atcgaaggaa ttaataatga tcgttcgagt tggagtaaac
     1501 tcagatacta tttcgcactc tatatcgata caacacaatt aagaaattta gcttatacaa
     1561 aattactatt tgttgattgc gttaaaggat tgcaaaaaca tcttagtgaa cttgagcagg
     1621 tgtaatcaga tggatctaaa aacatttaca gcacagatcg aactaatgca tcaagaagct
     1681 ttaagacaaa gcgcctcgta cgaagacaag tggctcaaca cgttccatgg tggacgtgag
     1741 agcgcacttg atcaagtact caaattattg aaaggggaac gtcgggatgg ataagaaagc
     1801 ggcaatgaaa cgaatcatcg aactgacaca ttctgagaat tggcaagaag acaaagaaat
     1861 agttacagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
     1921 gccgagaaag attgcaattt ggcatggtga tcgaattcta gtaacaggta ctgctgaaca
     1981 gttatctgaa attactggtc tgagcaaaaa catcatctgg gatagagcaa ggagcttatg
     2041 gattgattca aaaggacgac aatttaggta tgtggaggag aaaaaatgaa cgaactaatc
     2101 acaaaagtag agcagtgggc taaagacaag ggactggatc aagcagatcc aaaagcacag
     2161 tttttgaaag tagctgagga attcggggaa atcgcttcag caatggcaag aagtaatgat
     2221 gagctattta aagatagcgt aggagacgta atcgtcacgt tgattatcct ttccatgcaa
     2281 aaagggacaa acgtacaaga gtgtttagaa atggcataca acgaaatcaa agggcgcaca
     2341 ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt tggaggacag caaatgatag
     2401 gtttaacaat attttggtgt gcgattggtg gtttactagc atttctagga tactacctca
     2461 tctgtgaaag tagaaaagga agaaaatata cagtgacagg tattgtcctg ttaattatcg
     2521 gaattctagt tgcagttgga gcagaactga atctttatgt aaatggtaca caagaagatt
     2581 tggtgcgatt tatgttttgg atgaagaatt aggaggaaaa aaatgaacga aaacaaatta
     2641 atcaaattag gtgtagcagg agcagtaatc gtaggtattg gagttatcgg aggatttaag
     2701 ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa cggcggtatc
     2761 aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt gatccaatat
     2821 ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag cgacggcaaa
     2881 aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc agcaaaaatg
     2941 tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt aaaatctaag
     3001 cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt cctttcagga
     3061 gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt tgaatctaaa
     3121 gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga aacacaaaaa
     3181 tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct agatgcagaa
     3241 actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc agaagcagag
     3301 gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg ttatgaagaa
     3361 gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac tgtagttacg
     3421 gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt tcttaggttt
     3481 agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca aagaagatga
     3541 caacaacgat gaaatgaaat tttaggagga gaaataatgg atctcattac acaatacagt
     3601 gatatcatcc tcaagaaaat catggcaaag attcagaaag ataaaaaagc aaaagaacga
     3661 gcggaattag ttaagttgga aatggctgaa acaggag
//
LOCUS       DAZKAS010000197         3589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000197 DAZKAS010000000
VERSION     DAZKAS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.201"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8H7_002670"
     CDS             complement(254..541)
                     /locus_tag="ACG8H7_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP8984720.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8H7_002671"
     CDS             complement(561..785)
                     /locus_tag="ACG8H7_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFP8984721.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8H7_002672"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8H7_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984722.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8H7_002673"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8H7_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFP8984723.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8H7_002674"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8H7_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP8984724.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1014 a          646 c          548 g         1381 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctt
//
LOCUS       DAZKAS010000198         3550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000198 DAZKAS010000000
VERSION     DAZKAS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.202"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..1897
                     /locus_tag="ACG8H7_002675"
     CDS             131..1897
                     /locus_tag="ACG8H7_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFP8984725.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1912..2349
                     /locus_tag="ACG8H7_002676"
     CDS             1912..2349
                     /locus_tag="ACG8H7_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP8984726.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2362..3513
                     /locus_tag="ACG8H7_002677"
     CDS             2362..3513
                     /locus_tag="ACG8H7_002677"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP8984727.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1189 a          634 c          768 g          959 t
ORIGIN      
        1 ataccgaaat ctttttattt tgctgacata ctcaaacctt tggcacaaag tttcgtgcca
       61 aagtacgact atttctcttt ttagtaagcg gatacaatta gagtatcaaa aggagagagg
      121 gagagacaaa atggaacgga cgattcccaa aaacaacatt tcgctaaaag cgagggtaca
      181 aaaattaggc agtactttat caagcatggt catgccaaat attggagcac tgattgcctg
      241 gggtgtatta acggctctat ttatcccaga tggttattta ccaaatgagg ctttggctac
      301 catggtaagc ccaatgctaa cttatctgat tcctttattg atcggttata ctggcggaaa
      361 agtaattgct ggtgaccgcg gatcagttgt tggggcaatt gcaacgatgg gtgtgattgt
      421 cggaacagat attccaatga tgctaggcgc gatgatcatg ggacctcttg gtggatatgc
      481 catcaagaaa tttgatcagc tattccaaaa acggatcaaa tccggttttg aaatgctagt
      541 caataatttt tcagcaggtc ttatcggttt tggattagcg ttattaggtt tttctgcgat
      601 tggtccagtt gtcgatgcat tgactcaagc aatggcaaaa ggtgtagaga tcattttatc
      661 tgcccatttg attccattga caagtatttt tattgaacca gcaaaaatct tatttttgaa
      721 taatgcaatc aatcatggga ttttgacacc attgggaact gaacaagtag cagaggctgg
      781 aaaatctatt ctattcttgc ttgaagcaaa tcctggtcca ggacttggcg tattacttgc
      841 ttttactttg tttggcaaag gagcagcaaa atcttcagca ccaggagcca tgatcattca
      901 ctttttaggc gggatccatg aaatctattt tccttacgtt atgatgaaac cgttattatt
      961 cttatcagtt ataggcggag gaatgtcagg aagtttcatt ttccaattga tggatgctgg
     1021 actaagagcg ccagcttctc cagggtcgat cattgcgatt ctagcgatga caccgttcaa
     1081 ttcttacctt cctgttattt tgggcgtgat tgttgccgca gctgtttcgt ttggtatttc
     1141 tgcagtaatt ttgaaagcgg atgcgaaaga tacaaatgaa gacttcgaaa aatctgttca
     1201 agagacaaag gcagctaagc aagcttccaa aggtgcccaa ggtattcctc aaggagctac
     1261 attctctggc gtacaaaaaa tcatttttgc ttgtgatgca ggaatgggat caagtgcgat
     1321 gggcgcttcg atcttgagaa aaaaagtaca ggaagcagac ttgcctcaaa cggtgaccaa
     1381 tcaagcaatc agccaactga cagatgactc gaatacattg atcgttactc aggtcgaact
     1441 acaagaaaga gcaaaacaaa aagcaccgag cgcgcgtttt gtctcagtag aaaacttttt
     1501 gaattcacct cgatatgatg aaatcattca agaattaagc agcaagcaga aaacatcagt
     1561 ttctgaaatt gagaatgagc cagcagaaga gtttctttca gcaaacatca aacaaatcaa
     1621 agagattcta cttgtccacg atgatcgttc cggatcagca acaatgggga tgaccgtgct
     1681 caaagatata ttagacaaga accatttaac tatcccgtta agaaaagtca atatccaaga
     1741 tttaaaagat gaacctgatt cactgatcgt agccaaagaa agtttagcgg aaaaagtaca
     1801 gatggctgca cctcaatcca ttcatttatc agtcaacagc atggtcaatc cgcaaaaata
     1861 cgaatcgatt gcacaatatt tgaaacagac agcttaaggg aggaaaacag catgcaaagt
     1921 ttgccattcg acatgatcga gctcaatcga tcttttaaaa ccaaagaaga agcgatccgc
     1981 tattgcggtc agatgcttgt agaagcaggg tgcgttgatg aggaatatat cgatgccatg
     2041 gtagatagag acaaaatgtt atctgtttat atggggaatt ttatcgcgat tcctcatgga
     2101 acagatgaag caaaaaaata tgtgaaaaaa tccggcatct gtgtcgttca agtaccagat
     2161 ggtgtagatt ttggtaccga agaagaagaa aagattgcga ctgttttgtt cggtattgca
     2221 ggagtcggag atgagcattt gcaacttgta cagcaaatcg cactttattg cagtgatatg
     2281 gacaatgttg tccagctagc agacgcgctc agcaaagaag aagtaacgga aaatctagca
     2341 attgcttaag gagagagaaa aatgagagca gtacattttg gtgcaggaaa tatcggtcga
     2401 ggatttattg gagaggtttt ggcagctaat ggatttgaga ttactttcgt agatgtgaat
     2461 cacacgatca ttgatgcttt aaatgaaaga aaagaatacg aaatcgaatt agcagatgaa
     2521 tcaaaagaaa aaatcactgt aaaaaatgtc aaaggaatca acaatcaaac aaatccagaa
     2581 aaagtaatcc aagaaatcgc tgaagcagat ttagttacta ccgccattgg cccgaatatc
     2641 ttacctttta ttgctgagtt gattgcaaaa ggtatccaag aaagagaaaa ggaaggaagc
     2701 acaacatctt tagatatcat tgcctgtgaa aatatgattg gcggttcagc atttctagaa
     2761 aaagaagtgt ataaatatct tccggaaaca tcatttactg acaaatatat cggctttcca
     2821 aatgcagcag tcgatcgaat cgtcccatta caacaccatg aagatccttt attcgtccaa
     2881 gtggaacctt tcaaagaatg ggtcattgac gatagccaaa gaaaaaataa aaaaatccaa
     2941 ttaaaagggg ttttatatgt agatgatcta gaaccatata tcgaacgcaa actatttagt
     3001 gtcaatactg gtcatgcgac tgtcgcttat acaggtgcct tgttaggtta ccagaccatt
     3061 gatgaagcaa tgcaagatgc gttagttgtg gcacaattaa aatcagtttt gcaggaaaca
     3121 ggaagtttgc tgattgccaa atgggggttt gatgcagagc agcatcatgc gtatattgaa
     3181 aaaatcattc atcgcttcca aaacaaaaat atctctgatg cgatcacacg tgtcgctcgg
     3241 acaccattac gtaagttagg ttatcaagaa agatttacac gtccagtgag agaactacaa
     3301 gaacataact tgacttgccc acatttgacg gcaacaatgg ggattatttt taattactat
     3361 gatcctgaag atgaacaaag ccgtcaattg catgaaatga agatccatga aaacttggaa
     3421 caattgattc aagaagtcac aggaatcaat gatccgaaaa caatcgggaa tatcaagcaa
     3481 aacgtgaacc gttatgccaa gcaagtcgct taaaatagga tacgaaaaag gggaatgtga
     3541 cgaaattttt
//
LOCUS       DAZKAS010000199         3516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000199 DAZKAS010000000
VERSION     DAZKAS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.203"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1821
                     /gene="yfcC"
                     /locus_tag="ACG8H7_002678"
     CDS             82..1821
                     /gene="yfcC"
                     /locus_tag="ACG8H7_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP8984728.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1978..3162
                     /gene="iadA"
                     /locus_tag="ACG8H7_002679"
     CDS             1978..3162
                     /gene="iadA"
                     /locus_tag="ACG8H7_002679"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP8984729.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3273..3476
                     /locus_tag="ACG8H7_002680"
     CDS             3273..3476
                     /locus_tag="ACG8H7_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP8984730.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1116 a          590 c          817 g          993 t
ORIGIN      
        1 tgtcattttt actgtataat tcatttgatt atccattttt tattcatcaa aattactgaa
       61 aaaagaaagg aagaaataaa tttgaagaaa acaaattctt caggaaaacc gaaacgcgac
      121 ttcactaaat tatcgacacc ccatacgtat gtcattattt ttggtgtagt catctttgca
      181 tggatactga cttttgtcgt tccagcagga aagttcagca cgcaagatat tgaatacaaa
      241 gatgcaaatg gcgaaacaag tactcgtact gttttacgtc aagattcctt ccgttatgct
      301 tacgagctgg ataagtcgta tgtgtttgat caattagaag aactgcaaga tcatccagca
      361 gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa aagtcattgc tgacggagaa
      421 aaaaatctta cgcaggaaaa actagatgaa atctccttga cagatgatgt gctttacgat
      481 cagtacggcg aaaatatcta tgatacttct aaaaaattgc ataaaacagc caaaatatgg
      541 ggaacagatg attttggcgg ctttggtttc ttaaattttg tttttgaagg actagtatct
      601 ggagataagt atggatctgc tgtagggatc gctgcattga tccttgttgt tggaggtgct
      661 tttggtatta ttatgcgtac cggagcgatc gacgcaggta tctatgcgtt tatcagtaaa
      721 actaagggac tggagcgttt agctttaccg ctattattct ttgctttttc attcggtggc
      781 gctacattcg ggatggcaga agaggtaatt ccattctcaa tggtgatggt tccatttgtc
      841 attgcgttag gatatgactc gatcgtagca gtaaccgtga cttatgtcgc atctcaagtc
      901 ggaaatgcga cctcttggat gagcccgttt agcgtagcgg tggctcaagg gattgctgga
      961 atccccgtgt tgtctggtgc gacattccga ttgattatgt gggtagttgt tactgcatta
     1021 gccgctggtt atatgatgat atatgcagaa aaaatccgta aaaaaccaga aagttcgttg
     1081 acttacaaat cagatgatta tttccgttct catatcaaaa agacatctga tgaaaataaa
     1141 aaattcatgc ttgggcataa attgatctta ttagagatgt tagttgtcct tgtctggatc
     1201 gtttggggcg tgactcaaaa aggctattac attccagaaa tcgcttctca attctttgtg
     1261 atgggattag ttgctggaat cattgctgtt cttttcaaat tagatggaat gaaagtcaat
     1321 gacatcgctt cttctttcca atctggtgca gctgatttag ctggtacagc aatcgtagta
     1381 ggtatggcca aagggattct tcttgtcttg ggaggatctg atgcatctgt tccttcagct
     1441 ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg gtgttccagc tgtcattggt
     1501 gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg ttgttacatc aaattcagga
     1561 caagcagctt tgactatgcc aatcatggct ccactggcag atttagtagg tgtatccaga
     1621 caagtagcag ttcttgctta tcagcttggt tcaggtttta tggacgcttt tacacccgta
     1681 tcagccagct tgattggtgt gctaggtgtt gcacggatcg aatgggcaaa atgggcaaga
     1741 ttccaaatca agatgcaagg attcctgttt gttttaggaa ctattttcat catgattgcc
     1801 attgcaatag gtctgcagta gtactggaga cctgtaaata aaaaagtaat ccaaacagta
     1861 gataaggact ccaagaaccg tcattttacg gtaaaatcaa aggagtaaag atcctatcgt
     1921 ttggattatt ttttaagatc ctatcatcag ttatacttaa agaaagagga aaaatgtatg
     1981 aaaatcattc gacaaattga agtgtttgca ccagattact taggaaaaat ggatgtactg
     2041 attgccggtg ggaagatttt ggctgtagaa gaacagctag aaggcggcta cgaaggagtg
     2101 gaagtagaag aactttcagg tgagggaaaa gtactaaccc cgggtttcat tgactgtcat
     2161 ttccatattt taggtggagg aggagaaggc ggttatcaaa atcgaacgcc agaagtcaca
     2221 ttgagccaat tgactacagc tggtgtaacg acagttgtcg gttgtttggg aacagacgga
     2281 gaaggtagag atatgactgc gttgatcagc aaagcgaaag gtctggaagc agaaggaatc
     2341 tccacttatg tatacgaagg ttcgtaccga ttgccggtaa aaacagtaac agattctatc
     2401 atcaaagact ttttgacaat tgataaaatt atcggaatcg gagaaatcgc agtatccgat
     2461 caccgttctt ctcagccaag ttttgaagag ttcgctcgtg cagtagcaga tgctcgtgta
     2521 ggaggcatgt tatccggaaa agctgggatc atcaatgtcc acttaggcgg aggaaaaaga
     2581 aagctagagc tattgacacg aatcgtagaa gaaacagaaa ttccgatcac acaatttttg
     2641 cctactcatg caaatcgcac accagaatta tttgaagctt gcgtggcatt tgccaaacgt
     2701 ggtggcacga ttgacttcac tgctagcgaa gatcctgatt tttgggagaa gacagatggt
     2761 gaagttcgct tcagcaaagc actgaaacga ttgatcgagg aagatgtttc tttagataat
     2821 ttcacgatga cctcagatgg acaaggaagc ttgccttatt ttgatgaaaa caatcatttc
     2881 ctagggttag gagtaggaag tgccaaggca ttgctagtag ggatcaaaga agctgtgcaa
     2941 aaagaaagta tccctttgga aatcgcctta cgagctataa ccagtaatcc agcacgtata
     3001 ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg atgcagatct gtgtatcttg
     3061 gataaagaaa cgcttgatat tgatacagtt atcgctaaag gagaaataat ggtccaagaa
     3121 aaagaagtca aagtatgggg gactttcgaa aaaagttttt aatcaacagc tctaactatc
     3181 ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg ttgatgttac cctgaaggta
     3241 gatcaataaa caaactaaga ggaggaagca caatggcaga tttaaaagga cgttctaaag
     3301 atatgaaaga taaagtagtc ggtgaaggaa aagaagcata cggcaaagca acagacgaca
     3361 agagcaaaga aacagaaggg aaagcacaat cactaggtgc tgacatcaaa gaaaaagctc
     3421 gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa agacgaaaaa aaataaaaac
     3481 cacttattac taattgaatc aagcaaataa aaaatc
//
LOCUS       DAZKAS010000200         3494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000200 DAZKAS010000000
VERSION     DAZKAS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.204"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8H7_002681"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8H7_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFP8984731.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG8H7_002682"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG8H7_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          987 a          774 c          548 g         1185 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaac
//
LOCUS       DAZKAS010000201         3484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000201 DAZKAS010000000
VERSION     DAZKAS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.205"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(775..2229)
                     /locus_tag="ACG8H7_002683"
     CDS             complement(775..2229)
                     /locus_tag="ACG8H7_002683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984732.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2230..2529)
                     /locus_tag="ACG8H7_002684"
     CDS             complement(2230..2529)
                     /locus_tag="ACG8H7_002684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984733.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2832..3203)
                     /locus_tag="ACG8H7_002685"
     CDS             complement(2832..3203)
                     /locus_tag="ACG8H7_002685"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP8984734.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         1067 a          739 c          561 g         1117 t
ORIGIN      
        1 ttttttatgt aaagttctgt caaatatagt cactttatat tactttctct tgagtaaaaa
       61 aacatgtgag gtcgtaaaca aagattgaac tatctacatt catactctat aatctgttta
      121 tttgcagact gataatagca aaagaaaact tataatcaaa taatatcaac atttgatcac
      181 ataatttgag attgaatgct gatttaccta tgttttacgt aatggtcttt agttattttt
      241 gctcataaaa tgcataaaac acgcgttttt atgtgtgctt gaaatttctt ggctctataa
      301 tttctaagac tgataaaaat attctatcgg ttctttttgc aaataaaaaa acatatagaa
      361 gctccattag attgaatcac cacaacacaa tcaaaggagc attccgtatg cttgattttc
      421 attctataaa actactttta ggaatcaaag aaaatcacaa cggttcaaac taaaagcaag
      481 aatatccgaa gtaccattgt ttcatcgatc atgagaaaag cattcacctc tgtctgcaga
      541 tgttcttata tcgctctgtc agtcctatta ataaagtact gttttgtttt caaaaaaact
      601 attacgagta ctttttaaga gaatgccttg caaatattca tcacacaccg ttatataagt
      661 caaatagttt ctttcagtcg tcaagaaaag attctcctgt ccttggcatt atcagttaag
      721 tagcaactta attctcggac ttgttctact ctagcaaaat ttattttcat caacttacgt
      781 ccaaatgttt ttttgaataa ttacttcatc gcgggtctgg aagacttgcc tcattcgata
      841 agtcactagc ttttggcgtc cttcaggatt tctagtatac accaactgac atttgggcag
      901 cgacgattga tttaattcgt ttagaaaaag gttagcgatt tgcttatttc ggccaaagcg
      961 ttctggtacc gtcagaacta tctttttccc ttcgattaaa taccgcggat tctccagcgg
     1021 tgctaaaagc tccctcatag cttcagtaaa aagctgtcct tctcgatagc cagcattctc
     1081 aagatagcat ctaaattcct gctgatcagc cgctacgatt acttttctat cactaacttc
     1141 accaaggcgc tgcaacgtcg tttgaacgca tatggccaag ctttttttgc gggcacattc
     1201 cttttcccat agcttttgtc gcctacgaat ttggaaatat tccagcaagt tacctttaaa
     1261 tatagctgac cagaagctta gaggctctat attttttact actatcggca atgaagtttc
     1321 ttgtttttgc tttacctgat cgactttcct aacgacgatt ggcatagata ggtgccagcc
     1381 atcagctaaa gcagtactcc attgttcttt caaccgactt cgttcacaag aaaaatagaa
     1441 catcgactga gtataagttt ccatcgtggt ggtcgtatat tccagtggta aagcaaagcg
     1501 atcgccacca ctctcaattt ttggtgaaga ttcaaaggtc agacctgaaa tattgcccaa
     1561 acgccgtagc attcttttca aatcaggctg ttcagacaga ggaactcccg gtaatccaat
     1621 cgctaaatgc caaatgttcg ttactttatt ttgtgcacct aggcgtaagc cccgccctcg
     1681 tagttgttgt gtttgcacaa aagtggaagc ttgattagcc aagatcaaca gattaattgc
     1741 aggacaatcc cagccttctc ctagcaaact aacggttcct accaaaatat tcaacttctt
     1801 ttcggttaat aggcgagtaa ctgcttgcat caatgcttgg cggtttttgt catccaccgt
     1861 caaataaaaa taacccgaca tataaggatg ttcattcata cccgtcgcta gttctgccag
     1921 ctcaacagtt actagcattt tcggtaacag taagaactct ccacagatgg caccgatttg
     1981 ctgagtgtta cctaatagac gaacaacttc atcaaacaaa ggaaccacgc cataagctaa
     2041 ggtaccatca ctgcctgcta atacctcctt gcaaatacga tccatcaaaa tgaccgctgt
     2101 cagttcttgc ttcagctgaa cttcttcttt taacaaaatt tgacaaactg cttgcttttt
     2161 ctcaggaatc gcctgactac caacagtgtc tggatactct ggaaacaaat gcagcgactc
     2221 atcaaacaat catccagctt ttttcaattg ataaaaaata ggcaattccg gttgatcttc
     2281 aaacatctgc tgataaatag tgatcagctc ttttttacct aaagatggaa ctgaagcttt
     2341 tttaccttgc tgcttagaac tgcctaatag ctgccaatgt tcgggtatca attcatagtt
     2401 atgatgattc aagtaaatta atgcactagt gaacagctca aaattttcat aaatttcctt
     2461 cgaatgctca agcggatgct gaacaaattc taaagaaagc aaataatcca ccgtctcttg
     2521 gctttccatt aacgcttcca ccagctcatt ttgtttctct aaaaaggtaa aataatgctg
     2581 agtagtttcc tgattagccg gaatcaaata tagaaaatcc tgatgaggac atagaacctc
     2641 ctcctttacc aactcgggaa tgctaatttc ttcgtcaatc aggccatttt aactcaatat
     2701 attgcttcca ctccgtctga gagctatcaa tcggaggcgt ggctgtcaag gaaatcgtca
     2761 acagattcgg ttgttttttc ttcaagtcaa tcaacaattc accccagctt ttttttaaat
     2821 gatgagcttc atcaaaaaac taacagctcc agctgatcac cataagttgg ctcctccttc
     2881 agtcggttgt aaatggtctg atacgtttcc actgtaatat gactaggatg ttcaatcgcc
     2941 ccagaaagat agcaactatc tgctggattt actaagaaat attcctcgat tgttgttaac
     3001 cactgtttct tcaatattag ggaaggaacc aaaattaagg ttttacaatc caaacgtcgg
     3061 atcatctcaa ttcctaaaaa ggtcttgcca gcccctggtg gagccactag atggactgaa
     3121 ttgttattta aataagtagc aaagttagtc aatatacgct tctgatagct acgccactga
     3181 attttttcat cagatagcca catcccaggt ccccctctca cgtattttct ctatcataat
     3241 ctaaatgaat acagcaaaaa aaagatttct tcaagaatat gtgtttttat agtaatattt
     3301 tacttactct aaactcaaat gaaaaggtaa aaaaactggt agtacaatct tttaaattct
     3361 cattgttttg tacgttcttt actatcaaaa aaacaaaaaa aacgcgtttc tacgcgtgac
     3421 caaaattttt agctctataa tttctaagac tgatagaaaa tcattctgtt tttgaagtct
     3481 tttt
//
LOCUS       DAZKAS010000202         3261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000202 DAZKAS010000000
VERSION     DAZKAS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.207"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..693
                     /locus_tag="ACG8H7_002686"
     CDS             <1..693
                     /locus_tag="ACG8H7_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8984735.1"
                     /translation="KNDVVMYLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHIT
                     LDKAYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVI
                     HAPEDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIP
                     KDKLAAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVEL
                     PAMPDLVDKRRS"
     gene            908..1876
                     /locus_tag="ACG8H7_002687"
     CDS             908..1876
                     /locus_tag="ACG8H7_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP8984736.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1869..2900
                     /gene="vanA"
                     /locus_tag="ACG8H7_002688"
     CDS             1869..2900
                     /gene="vanA"
                     /locus_tag="ACG8H7_002688"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP8984737.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2906..>3261
                     /locus_tag="ACG8H7_002689"
     CDS             2906..>3261
                     /locus_tag="ACG8H7_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFP8984738.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT          966 a          640 c          798 g          857 t
ORIGIN      
        1 aaaaatgacg ttgttatgta cttggcgcac gatattaaaa cgccccttac atccattatc
       61 ggttatttga gcctgcttga cgaggctcca gacatgccgg tagatcaaaa ggcaaagtat
      121 gtgcatatca cgttggacaa agcgtatcga ctcgaacagc taatcgacga gttttttgag
      181 attacacggt ataacctaca aacgataacg ctaacaaaaa cgcacataga cctatactat
      241 atgctggtgc agatgaccga tgaattttat cctcagcttt ccgcacatgg aaaacaggcg
      301 gttattcacg cccccgagga tctgaccgtg tccggcgacc ctgataaact cgcgagagtc
      361 tttaacaaca ttttgaaaaa cgccgctgca tacagtgagg ataacagcat cattgacatt
      421 accgcgggcc tctccgggga tgtggtgtca atcgaattca agaacactgg aagcatccca
      481 aaagataagc tagctgccat atttgaaaag ttctataggc tggacaatgc tcgttcttcc
      541 gatacgggtg gcgcgggact tggattggcg attgcaaaag aaattattgt tcagcatgga
      601 gggcagattt acgcggaaag caatgataac tatacgacgt ttagggtaga gcttccagcg
      661 atgccagact tggttgataa aaggaggtcc taagagatgt atataatttt ttaggaaaat
      721 ctcaaggtta tctttacttt ttcttaggaa attaacaatt taatattaag aaacggctcg
      781 ttcttacacg gtagacttaa taccgtaaga acgagccgtt ttcgttcttc agagaaagat
      841 ttgacaagat taccattggc atccccgttt tatttggtgc ctttcacaga aagggttggt
      901 cttaattatg aataacatcg gcattactgt ttatggatgt gagcaggatg aggcagatgc
      961 attccatgct ctttcgcctc gctttggcgt tatggcaacg ataattaacg ccaacgtgtc
     1021 ggaatccaac gccaaatccg cgcctttcaa tcaatgtatc agtgtgggac ataaatcaga
     1081 gatttccgcc tctattcttc ttgcgctgaa gagagccggt gtgaaatata tttctacccg
     1141 aagcatcggc tgcaatcata tagatacaac tgctgctaag agaatgggca tcactgtcga
     1201 caatgtggcg tactcgccgg atagcgttgc cgattatact atgatgctaa ttcttatggc
     1261 agtacgcaac gtaaaatcga ttgtgcgctc tgtggaaaaa catgatttca ggttggacag
     1321 cgaccgtggc aaggtactca gcgacatgac agttggtgtg gtgggaacgg gccagatagg
     1381 caaagcggtt attgagcggc tgcgaggatt tggatgtaaa gtgttggctt atagtcgcag
     1441 ccgaagtata gaggtaaact atgtaccgtt tgatgagttg ctgcaaaata gcgatatcgt
     1501 tacgcttcat gtgccgctca atacggatac gcactatatt atcagccacg aacaaataca
     1561 gagaatgaag caaggagcat ttcttatcaa tactgggcgc ggtccacttg tagataccta
     1621 tgagttggtt aaagcattag aaaacgggaa actgggcggt gccgcattgg atgtattgga
     1681 aggagaggaa gagtttttct actctgattg cacccaaaaa ccaattgata atcaattttt
     1741 acttaaactt caaagaatgc ctaacgtgat aatcacaccg catacggcct attataccga
     1801 gcaagcgttg cgtgataccg ttgaaaaaac cattaaaaac tgtttggatt ttgaaaggag
     1861 acaggagcat gaatagaata aaagttgcaa tactgtttgg gggttgctca gaggagcatg
     1921 acgtatcggt aaaatctgca atagagatag ccgctaacat taataaagaa aaatacgagc
     1981 cgttatacat tggaattacg aaatctggtg tatggaaaat gtgcgaaaaa ccttgcgcgg
     2041 aatgggaaaa cgacaattgc tattcagctg tactctcgcc ggataaaaaa atgcacggat
     2101 tacttgttaa aaagaaccat gaatatgaaa tcaaccatgt tgatgtagca ttttcagctt
     2161 tgcatggcaa gtcaggtgaa gatggatcca tacaaggtct gtttgaattg tccggtatcc
     2221 cttttgtagg ctgcgatatt caaagctcag caatttgtat ggacaaatcg ttgacataca
     2281 tcgttgcgaa aaatgctggg atagctactc ccgccttttg ggttattaat aaagatgata
     2341 ggccggtggc agctacgttt acctatcctg tttttgttaa gccggcgcgt tcaggctcat
     2401 ccttcggtgt gaaaaaagtc aatagcgcgg acgaattgga ctacgcaatt gaatcggcaa
     2461 gacaatatga cagcaaaatc ttaattgagc aggctgtttc gggctgtgag gtcggttgtg
     2521 cggtattggg aaacagtgcc gcgttagttg ttggcgaggt ggaccaaatc aggctgcagt
     2581 acggaatctt tcgtattcat caggaagtcg agccggaaaa aggctctgaa aacgcagtta
     2641 taaccgttcc cgcagacctt tcagcagagg agcgaggacg gatacaggaa acggcaaaaa
     2701 aaatatataa agcgctcggc tgtagaggtc tagcccgtgt ggatatgttt ttacaagata
     2761 acggccgcat tgtactgaac gaagtcaata ctctgcccgg tttcacgtca tacagtcgtt
     2821 atccccgtat gatggccgct gcaggtattg cacttcccga actgattgac cgcttgatcg
     2881 tattagcgtt aaaggggtga taagcatgga aataggattt acttttttag atgaaatagt
     2941 acacggtgtt cgttgggacg ctaaatatgc cacttgggat aatttcaccg gaaaaccggt
     3001 tgacggttat gaagtaaatc gcattgtagg gacatacgag ttggctgaat cgcttttgaa
     3061 ggcaaaagaa ctggctgcta cccaagggta cggattgctt ctatgggacg gttaccgtcc
     3121 taagcgtgct gtaaactgtt ttatgcaatg ggctgcacag ccggaaaata acctgacaaa
     3181 ggaaagttat tatcccaata ttgaccgaac tgagatgatt tcaaaaggat acgtggcttc
     3241 aaaatcaagc catagccgcg g
//
LOCUS       DAZKAS010000203         3232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000203 DAZKAS010000000
VERSION     DAZKAS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.208"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACG8H7_002690"
     CDS             complement(554..2167)
                     /locus_tag="ACG8H7_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP8984739.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACG8H7_002691"
     CDS             complement(2308..3216)
                     /locus_tag="ACG8H7_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8984740.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          556 c          572 g         1066 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt tt
//
LOCUS       DAZKAS010000204         3221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000204 DAZKAS010000000
VERSION     DAZKAS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.209"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8H7_002692"
     CDS             301..585
                     /locus_tag="ACG8H7_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984741.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8H7_002693"
     CDS             588..1004
                     /locus_tag="ACG8H7_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984742.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8H7_002694"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8H7_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP8984743.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG8H7_002695"
     CDS             2544..2630
                     /locus_tag="ACG8H7_002695"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFP8984744.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG8H7_002696"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG8H7_002696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984745.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZKAS010000205         3196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000205 DAZKAS010000000
VERSION     DAZKAS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.210"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..698
                     /locus_tag="ACG8H7_002697"
     CDS             <1..698
                     /locus_tag="ACG8H7_002697"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP8984746.1"
                     /translation="SNCQAVKQLAQKHALDIIAFTPESIIYPYNIAAPETMQWEKSKA
                     YFRNAILATAELGCSLMTINSGYGLMEEPIEESWKRSREMLRYLAEIAETEGVTIALE
                     TLRPEESKIVTKLQEAQRMVHDVAHPSLKLMVDTAAMGVAGEGLQDWFKAFGDDIVHL
                     HFVDGTPEGHLAWGDGNFNLHEFLTILEKYDYQGFLGQEITDSRYLKRPYLADKQVFD
                     KLSEALRKREGNV"
     gene            695..1483
                     /locus_tag="ACG8H7_002698"
     CDS             695..1483
                     /locus_tag="ACG8H7_002698"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFP8984747.1"
                     /translation="MKDFVAVGYACMDIYDDLGKKYPTGNGVDVIAHLSKAGYDVALV
                     SAVGDDTYGKEFIEFCEDMEFDTSHVHVEEGDTAQIHLSLIDGDRVHGKVIDGVLANF
                     SLTKEDRAFIKEFKYLHTDYFGRIYGMLGEFQQAGCKIVFDFSSFINDKDFEMVAPYV
                     DIGQISVEGFSQETKKIMQYMHCLGIKICIATAGSQGALAYDGKNFYCQEALDPQEIV
                     NTVGCGDAFLAGFLMGDAASKPIEECLLLGAKSSQKIIQKFEPY"
     gene            1504..2367
                     /locus_tag="ACG8H7_002699"
     CDS             1504..2367
                     /locus_tag="ACG8H7_002699"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP8984748.1"
                     /translation="MTIDTCFHPIYFDMIQTSEAELKKREEAFGVHKQSPYTMDEILS
                     EMDYGKIEKSILHPIALTTTENVSIVTNEEISKIIQQEPNRFIGFASVDPNNPEAETE
                     LEQAFQKGLAGLTFHPGKQQIYPNDPKMKPLYQLCEKYDKPIYFHSGLSWEPRSYTKF
                     CQPLFVEDVLIEHPALRICLAHFSWPWIQEVMMLMIKYSNLYTDTSMLYLNSFDDFFG
                     DFFSNQLTPSTIERNFPRQIMFGSNTPRFRAFKVKRALEKIPFSKEVRENILTNNAYD
                     YVYGRKSRHDL"
     gene            2357..2785
                     /locus_tag="ACG8H7_002700"
     CDS             2357..2785
                     /locus_tag="ACG8H7_002700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP8984749.1"
                     /translation="MIYKEYLEFFSTEDVQLINITEEVEKVMARSNITSGVAFILSNH
                     TTTGITINEPLECVEKDIKNTLERLIPENSNYIHAHFLPSYGATSNNSPGHLKSSLLG
                     NHAMFSVEDGKLEKGSAQDIYLVECDGPQTRKILIKILGE"
     gene            complement(3084..>3196)
                     /locus_tag="ACG8H7_002701"
     CDS             complement(3084..>3196)
                     /locus_tag="ACG8H7_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8984750.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         1031 a          540 c          681 g          944 t
ORIGIN      
        1 attctaattg ccaagccgtt aagcaactag cacagaaaca tgctcttgat atcattgctt
       61 ttactccaga atcaattatc tacccttata acattgctgc gccagagacg atgcagtggg
      121 aaaaaagcaa ggcctatttt cgaaatgcaa tattagctac ggctgagtta ggctgctcac
      181 tgatgacaat caactccgga tatggtttga tggaagagcc tatcgaagaa agctggaagc
      241 gctcaagaga gatgctacga tacctcgctg aaatagcaga aactgaggga gtcacgattg
      301 cattggaaac gttgcgtccc gaagagtcga aaattgtaac caaattacag gaggctcagc
      361 gaatggttca tgatgtggca cacccatctt tgaagctgat ggttgatacc gcagcaatgg
      421 gggtcgctgg agaaggtctt caagattggt ttaaagcctt cggggatgat attgtccacc
      481 ttcattttgt tgatggaaca ccagaaggac atcttgcctg gggagacgga aattttaatt
      541 tacatgagtt tcttactatt ttagaaaaat atgattatca gggatttctg ggacaagaaa
      601 tcacagatag ccgctatcta aaacggcctt atcttgcaga caaacaagta tttgataaat
      661 tatcagaagc attgaggaaa agagagggaa atgtatgaag gattttgttg ctgtaggcta
      721 tgcatgtatg gatatctatg acgatttagg gaagaaatac cccactggaa atggagtgga
      781 tgttatagct catctctcaa aagctggtta cgatgtagct cttgtttcag cggtaggaga
      841 cgacacttat ggtaaagaat tcattgaatt ttgcgaggat atggaatttg atacttctca
      901 tgttcatgta gaggagggag atacagcaca aatacacttg tctctgatag atggggatcg
      961 agttcatgga aaagtaattg atggtgtact agcaaatttt tccttaacaa aagaggaccg
     1021 ggcattcatc aaagagttca agtatctaca cacagattat tttggccgca tctatgggat
     1081 gctaggagag tttcaacaag ccggctgtaa aattgtattt gatttttctt cttttatcaa
     1141 tgataaggat tttgaaatgg ttgctcccta tgttgatatt ggacagattt ctgtagaagg
     1201 attctctcag gaaactaaaa aaattatgca gtatatgcat tgtttaggaa tcaaaatctg
     1261 cattgccact gctggaagtc aaggggctct cgcctacgat ggaaagaatt tttattgtca
     1321 agaagcgctt gatcctcaag aaattgtcaa tacagtgggg tgtggggatg ctttcctcgc
     1381 gggtttccta atgggagatg cagctagcaa acctattgaa gaatgtttgt tattgggagc
     1441 taaaagttca caaaagatca ttcagaaatt tgaaccttat taattgacag aggagcggaa
     1501 gtaatgacca tagatacttg ttttcatcct atctatttcg atatgattca aacatcagaa
     1561 gcagaattaa aaaaaagaga agaggctttc ggtgtacaca aacaaagccc ctatacaatg
     1621 gacgaaattc tttcggagat ggattatgga aagatagaaa aatcaattct tcatccaatt
     1681 gctctaacga ctaccgaaaa tgtcagcatt gtgacaaatg aggagattag caaaattatc
     1741 caacaggagc caaatcgttt tattggtttt gccagtgtag atcccaataa tcccgaggca
     1801 gagaccgaac tggaacaagc ctttcaaaag ggacttgccg gtttaacttt ccatcctggg
     1861 aaacagcaaa tttatccgaa tgatcctaaa atgaaaccgc tttaccagtt atgtgaaaaa
     1921 tatgacaagc caatttattt tcactctggt cttagctggg agccaagaag ctacacaaag
     1981 ttctgtcagc cactatttgt ggaagatgta ttgattgaac atcctgcgct tcgaatctgt
     2041 ttagcacatt tttcatggcc ctggattcaa gaagtgatga tgttgatgat aaaatattca
     2101 aatttgtata ctgatacctc tatgctttat ttgaactctt ttgatgactt ctttggagat
     2161 ttttttagca atcaattgac tccctcaaca attgagcgaa attttccaag gcaaattatg
     2221 tttggatcaa atacaccacg ttttcgagcc ttcaaagtga aacgggctct ggaaaagatt
     2281 cccttctcaa aagaagtgag agaaaacatc ttgacaaata acgcttatga ttatgtatat
     2341 gggaggaaaa gtaggcatga tctataaaga atacttagaa ttcttttcca cagaggatgt
     2401 ccagttaata aatattactg aagaggtaga aaaggttatg gcgcggagca atataacaag
     2461 tggcgttgcc tttatcctta gtaaccatac aacaacgggc atcacaatca atgagccact
     2521 ggaatgcgtg gagaaggaca taaagaatac actagaacga ttaatccctg agaattcgaa
     2581 ctatattcat gcacattttc ttccctcata tggagcgaca agtaataact ctccaggaca
     2641 tttaaaatcc tccttactag gaaatcatgc aatgttttca gtggaggatg gtaagctgga
     2701 gaaggggagt gcccaagata tttatttagt agaatgtgat ggacctcaaa ctcgcaaaat
     2761 tctaataaaa atactaggag agtagttttg aattaccgga agggactggg acaaaagttt
     2821 tttcttaaaa aatagtccca tttcttcagt attaattaaa taaaaagtaa aatacattcc
     2881 cgaaactatt ggatgaagat cccagtagtt tcgggaatgt attttttcta gaggctttct
     2941 tttgctcttt gtcaagaagt gttgatgacg agattttcta attcaaatga aggataattt
     3001 cacaatctat taagcagtgg ctaggaacca attggttcct aagttgctgc ttttatttct
     3061 aataatttat attctatgat ttgtcaaatt aagctagaat aaaatgcttc ttgtacatag
     3121 ctcaaaaata tttcgattgg tgttctgtaa ttcaatgatt ttcttggaat atgattacgt
     3181 tgattgctga cactgg
//
LOCUS       DAZKAS010000206         3163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000206 DAZKAS010000000
VERSION     DAZKAS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.211"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..818)
                     /locus_tag="ACG8H7_002702"
     CDS             complement(<1..818)
                     /locus_tag="ACG8H7_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFP8984751.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
     gene            complement(832..2049)
                     /locus_tag="ACG8H7_002703"
     CDS             complement(832..2049)
                     /locus_tag="ACG8H7_002703"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP8984752.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2039..>3163)
                     /locus_tag="ACG8H7_002704"
     CDS             complement(2039..>3163)
                     /locus_tag="ACG8H7_002704"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP8984753.1"
                     /translation="IKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          878 a          677 c          529 g         1079 t
ORIGIN      
        1 acttgataag gatgtaacac catgagggtt ttattgtctt gatcttcact gacaatcgta
       61 taatccgcaa tcaagcgatg ggcatctggg atatttagca cttgtttgca aaactcatat
      121 aagttttcta cctttttatt atctttcgtt cgccaactaa ataaaaactt aggatgcaaa
      181 tccttcggta aggcatttgc aaaataccga gtcgtttgtt cattggaaac cacaaacaac
      241 tgtaaggtcg aaaagatatt gttccgaaac atgccttctt cagcatattt tttgatttga
      301 ttaaacgcct gataaaaacc atcttttgca gttacttgtt ttagctcgat ttggataatc
      361 ggtaagccat tgatcaataa ggtgacgtca aaacgtcgat cccgcgcttc tatgttactt
      421 ccacgttttg caatttgatg gaccacttca taagtagaga ttcctccacc aatgtcttga
      481 ttggaataaa ggataagtga gacagaaccc aattgcggat tttcccgttc aatcgtgatc
      541 cgagccatcc cattttcacc ttttaaccat ttcgcagcat caaatggggt tttcgtccgt
      601 agcaataatt cggttttgat cgtttcaaac tctttatcgg tcaaaggaga atcattcagt
      661 tctgcttgat tatttgagat gattttttga cgtaaattgc tccaaagatc ttcttctgat
      721 tttagatccg gacgatacgt ccactgatta tgtccttccc cgaggacacg aatcaactgt
      781 tcttcgactt ccgcttcctc acggtgacta attttcattc ttcttccctc cctaaacaaa
      841 catcttttgc aagaagcctt ttttcgtttc cttcagcaaa tctaacttac gttgatgaag
      901 agcgatcgta tcatctagct gcttgaagaa ggcaccaatt ttttgttgtt catttatatc
      961 cggtggaaac tgaaatgtcc tttgttctat accaccttta gtcacatgta ccattgtcgt
     1021 tccgttagtt gattctttaa ttttctcttt atcagtcaat agccaagtat atagatactg
     1081 tttgtccaat tccttagata aaaatttaag tttccaaata tgataatggt agattacacg
     1141 ttcttcattc cacatttcag gaccgaagct tgttgcccat aaatataaga gatctccttt
     1201 attagcatac ttgtcttcct ctaattctaa gtttgaataa taccatctat cattagtatt
     1261 aaaattacct actcttaaaa ccctatattt tccagaatca agtaattctt gttgtttaaa
     1321 agctctacca ttcaaaaaaa ctaccacttc ccccaacttc cgctcttccc aatctcccgt
     1381 aaatcctgga aaacgaactt ctggcacttt tgctccgttt ttagggaaca ttttttgtaa
     1441 gaatcctttt ttcgtttcct tcagcaaatc taattcgtgc tggtgaagca tgatggtgtg
     1501 atcaagttgt ttgaaaaatg aaccgatctt gatctgttcg tcataagttg gaagtgagat
     1561 aggtgtcttt ttaccactat caattgtata ggttccgacg gttccaatgc ctgcaacgtc
     1621 acggatatac ttctgaattt tgttatttat gaacgagtag taaaagaagt ttccgtcaat
     1681 tttatgctca ttcttgaacc aaatgatgtt tccatcctta aagtaaattg ggttatcatc
     1741 agtgattaat aaggggacac ctattgatcc gacaccagtt acaagcaagt ctccttgaga
     1801 aactttacct gaatttttcg aatattcatt atatttttct tctgaaatgt aaagataatc
     1861 ggatggttcc tcattttttg cagctgagac aatatcacga gcacgtaaaa atctaatacc
     1921 tgagttagtc caatctgatt gatgaattcg cttcacagaa gtaacatcca tcatatctcc
     1981 taacttacgc tcttcccaat cttccgtaaa accaggaaac cggatttctg gtcgtgtatc
     2041 attgctcatg tttgaacacc tccagcgcac cttgtaacca cgcttcattt tcaggtgttg
     2101 tttgcaggga agagatagct tctaaaagtt ctttttctag cacttgctgt tcttgtcgta
     2161 tcttcttgat gtcatttccg acatgaacca tatcaattgg ttcttcttct tcgaaagtat
     2221 cgacatagcg tgggatattt aagttatagt cattttcttt gatttcatca aaggttgcca
     2281 aatgagcgta tttctcgaca tcttttcttt cagcgtaggt ttcgaggatt ttttgaatgt
     2341 tttcttccga taatttattc tgatttttcc ctttaacaaa ttcacgacta gcatcaatga
     2401 ataatacatc tcgagtttga cgattcttct tcaatacaat caccgtcgtt ggaatcgatg
     2461 taccaaagaa caagttcgca ggcataccga tcacagcata aatactgccg tcttctaata
     2521 atttttgacg aatgacccct tctgcagcac cacggaataa aaccccgtgt ggcaagacga
     2581 tagccatagt ccctgtttcc ttcaaatgat agaaaccatg aagaagaaac gcaaaatctg
     2641 ctttggattt tggcgctaat ttcccgtagc gattaaaccg agaatcatct aagaaggtcg
     2701 tatctgctga ccaattagcc gaataaggtg gattcatcac tactgcatca aatgtgtaag
     2761 gttcatctgt tggccagtcc ttattcaacg tatccccatt gcggagattc atttcttcgg
     2821 catctacccc atgtaaaatc aagttcattt ttgctagatt gtatgtcgtt gtatttagtt
     2881 cttgcccatg atatttaacg ttatctggat gagttaagta gttacgtaca tttaacatca
     2941 aggaacctga acccatggtt gggtcaaaga cactaaagaa tcgtcgttct ttttgatcca
     3001 acgtcacgat ttgtgccatc atatcagaaa ccatatgagg cgtgtaaaat tctccggctt
     3061 tcttccctgc ttctgaagca aattgactga tcagaaattc atacgcatcc ccgatcacat
     3121 ccccatcgtg acctaaaact tccacgtcat tcaatttctt gat
//
LOCUS       DAZKAS010000207         3162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000207 DAZKAS010000000
VERSION     DAZKAS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.212"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG8H7_002705"
     CDS             <1..1125
                     /locus_tag="ACG8H7_002705"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP8984754.1"
                     /translation="IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1115..2332
                     /locus_tag="ACG8H7_002706"
     CDS             1115..2332
                     /locus_tag="ACG8H7_002706"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP8984755.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2345..>3162
                     /locus_tag="ACG8H7_002707"
     CDS             2345..>3162
                     /locus_tag="ACG8H7_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFP8984756.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
BASE COUNT         1089 a          534 c          658 g          881 t
ORIGIN      
        1 atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg
       61 tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac
      121 acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga
      181 cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt
      241 aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca
      301 tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc
      361 cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca
      421 gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat
      481 tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt
      541 cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga
      601 gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt
      661 atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg
      721 gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt
      781 gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca aaaaatcctc
      841 gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc atttggcaac ctttgatgaa
      901 atcaaagaaa atgactataa cttaaatatc ccacgctatg tagatacttt cgaagaagaa
      961 gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca agaacagcaa
     1021 gtgctagaaa aaaaactttt agaagctatc tcttccctgc aaacaacacc tgaaaatgaa
     1081 gcgtggttac aaggtgcgct ggaggtgttc aaacatgagc aatgatacac aaccagaaat
     1141 ccgtttccca ggatttacag atgattggga acagaggaag ttgggggact tcgttgtaga
     1201 ttatgttgaa aagacaagtg tacaaaatca atttcctatg ctaacttcat ctcaacaaaa
     1261 agggatagtt ttgcaagaag actattttgc aaatcggcaa gtaactacag aaaataatat
     1321 tggttacttt gtactaccta gaggatattt cacgttccgt agtcgaagtg ataatgatgt
     1381 gtttgtgttt aaccgtaatg acattattga tagagggatt ataagctatt tttatcctgt
     1441 atttacttta aaatctgctg attccgactt cttcttaaga cgtattaaca atggaatcca
     1501 acgacaatta tctattcaag cagagggaac aggtcaacat gttctgtctc taaaaaagtt
     1561 taaaaatata gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg gcaccttctt
     1621 caaacaactt gacgacacga tcgctcttca tcagcgtaag ttagatttgc tgaaggaaac
     1681 gaaaaaagga ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc cagaaatccg
     1741 gtttcctggt tttacggaag attgggaaga gcgtaagttg ggagaattag gtaaaactca
     1801 atcgggaatt ggatttcctg atgccgagca aggtggttct gaaggtactc cattttttaa
     1861 agtttccgac atgaataaca ttggtaatga gtatgaaatg agaaatgcaa atcattatgt
     1921 atctaatgag caaattgagc gtaaaaaatg gaagccaata aaagatgtgc cagcgattat
     1981 atttgctaaa gttggcgcag caataatgct taatagaaaa cgtttggtca caagcccttt
     2041 cttaatagat aacaatacca tggcttattt atttaataat acttgggata tctattttgg
     2101 taaaatattg tttgaaacta taaatttgcc aagatactcc caagtagggg cattgccttc
     2161 ttataacagt tctgatattg agaatatcag tgttaaggtt cctgtaaagg atgaacaaca
     2221 aaaaatcggt actttcttca aacagctaga tgatacgatc gctcttcatc aacgtaagtt
     2281 agatttacta aaagaaacga aaaaaagctt cttacaaaag atgtttgtct aggagggaag
     2341 aagaatgaaa attagtcacc gtgaggaagc agaagtcgaa gaacagttga ttcgtgtact
     2401 tggagaaggg cataatcagt ggacgtatcg tccggatcta aaatcagaag aagacctttg
     2461 ggtcaattta cgccaaaaaa ttatctcaaa taatcaagca gaactgaatg attttccttt
     2521 gaccgataaa gaatttgaaa cgatcaaaac ggaattactg ctgcgaacga aaaccccatt
     2581 tgatgctgcg aaatggttaa aaggtgaaaa tgggatggct cggatcacga ttgaacggga
     2641 agattcgcaa ttgggttctg tctcacttat cctttattcc aatcaagaca ttggtggagg
     2701 aatctctact tatgaagtgg tccatcaaat tgcaaaacgt ggaagtaaca tagaagcacg
     2761 ggatcgacgt tttgacgtca ccttattaat caatggatta ccgattgtcc aaatcgagct
     2821 gaaacaagta actgcaaaag atggtgttta tcaggcattt aaccaaatca aaaaatatgc
     2881 agaagaaggt atgtttcgga acaatatctt ttcgacctta cagttgtttg tggtttccaa
     2941 tgaacaaacc actcggtatt ttgcaaatgc cttaccgaaa gacttgcacc ctaagttttt
     3001 atttagttgg cgaacgaaag acaatgaaaa agtagaaaac ttatatgagt tttgtaaaca
     3061 agtgttaaat atcccggatg ctcatcgttt gattgcggat tatacaatcg tcagtgaaga
     3121 tcaagacaat aaaaccctca tggtgttaca tccttatcaa gt
//
LOCUS       DAZKAS010000208         3154 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000208 DAZKAS010000000
VERSION     DAZKAS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.213"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..797
                     /gene="fabI"
                     /locus_tag="ACG8H7_002708"
     CDS             42..797
                     /gene="fabI"
                     /locus_tag="ACG8H7_002708"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP8984757.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(907..1959)
                     /locus_tag="ACG8H7_002709"
     CDS             complement(907..1959)
                     /locus_tag="ACG8H7_002709"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFP8984758.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2085..3005
                     /locus_tag="ACG8H7_002710"
     CDS             2085..3005
                     /locus_tag="ACG8H7_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFP8984759.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          929 a          640 c          604 g          981 t
ORIGIN      
        1 tacgactatc aaatttttat cagaaacaaa ggagagtcat tatgtcattt ctagaaggaa
       61 aaaaaatcgt cattatgggc gtcgctaaca aaaaaagcat tgcttggggt tgtgctgaag
      121 caatgttgaa acaaggggca gaaattatct atacttacca aaatgaacga atgaaaaaat
      181 cattagtcaa attggtcggc gaagaagcat tcactgtaga atgtgatgtt tctagcgacg
      241 aaagcatcga aaaagcaatg aatgccatcg gtgaatatgc tggggagatc cacggccttg
      301 tccatgcagt tgcttacgcc aacaaagaag aattgtccgg aaatgtgtct gatatttcca
      361 gagacggcta tcaattagct caagatatca gcagctactc tttaattgct gtatcaaaat
      421 acgcacaacc gtttcttgca ccaaacagcg ggatcgttag catgacttat ctaggttcag
      481 aacgcgcaat cccgaattat aacatgatgg gtattgcgaa agcttctctt gaagctgcta
      541 tccgttattt ggcagctgag ttctcgccta aaggaattcg ggtcaatgga atctcagctg
      601 gggcaatcaa aacattagct gtcacaggtg ttaaagacta tcaaaaactg atccagttat
      661 ctgaatctcg tacgcctgat cagcaaggtg tgacgatcga agaagtcggt aatgcttgtg
      721 ctttcttaat cagcccactt tccagcggaa tcatcggtga tattatctac gttgacaaag
      781 gtgtccatct ttcataaact tttgttgcat tctttaaagt aaaacatata aaaatcagga
      841 aaaaactttt taaagtctct tcctgatttt ttctttttac gtttttttca actcattact
      901 ttgaaattat tccacgactt caatccagcc ttctggtgct tctatgtctc caaattgaat
      961 acctgtcaat tcatcataca accgttttgt tactggtcct acttccgttt cactataaaa
     1021 tacatgaaga ttgttattgt gataaatccc accgattgga gaaataacag ccgctgtacc
     1081 acatgcacca gcttctgcaa attgatctag atcatcaatg aaaatatctc cttcaattgc
     1141 ttgcagtcct agtcgttctt cagctaactg caacaaggaa tatttcgtga tacttggtaa
     1201 gatagagggc gattgcggag tgatgaattt tccgtctttt gtaattccaa agaaatttgc
     1261 tgcacctact tcttcgattt tcgtatgtgt ggctggatca agataaacac aatctgagta
     1321 atccagttct tttgcttctg ttcctggaag taaactcgct gcgtaatttc cgccgacttt
     1381 tgctgcacca gtccccttag gagcagcacg atcgtaatca gatacaataa aattcgttgg
     1441 ttttagtcca cctttgaaat acgctcctac tggcgtacag aagacgacaa ataaatattc
     1501 tggagctgga tggacaccaa tgttttcacc gaccccaatc agtaaagggc gcaagtataa
     1561 tgtagcgcct gttccatagg gcggcacaaa atcttcattt gcttttacca cttgcttgac
     1621 agcatcaacg aacatctttt cagggaccat tggcatcagc aatcgtctag cactttgatt
     1681 catccgtcga ctattttgtt caggtcgaaa gagattgacc ttgccgtctt ttcgacgata
     1741 agcttttaga ccttcaaaac attgctgacc ataatgaagc gctggagagc cttcatgaat
     1801 cgtcaggtaa ttatcttctg tcaattctcc ttcttcccat tgtccgtctt tccatttggc
     1861 aataaaacgc caaggagttt tgatataaga aaaacctaga ttgttccaat cgatttgcat
     1921 atacttacca ccttcgttat tttctccaca ttttatcatt atccatatta tttgtcattt
     1981 tattttcagc ttttttcctt cattatagtt atttttgcta tttccttttt tgaaaacttc
     2041 ttggtacaat acagaaagaa tcaaatttta gaaaagagga ttttatgttt tcattattag
     2101 caactgccgg tacgacagac tcttctggcg agacattgac agatcaagct gtcagcaaag
     2161 taaatgcgtt tcagcggttt tgggacggga ttgattggga cgccattttg gctgccttta
     2221 tccaaaaaag tttatatctg atttttttga tcgtcctttt ttggattctt caccgtgtgg
     2281 gaaaatatct gattgataag tcttttaatc agtattctaa aaaagccatt ctaaacgaga
     2341 gccgcttaaa gactttacat agtcttttaa atactatttt tcattatacc cttggtttct
     2401 tttttatcta tgcaatttta tctgctatcg gtgtgccgat cggatcactt ttggctggtg
     2461 ccggaattgc aggtgtggca atcggtcttg gtgcccaagg gttcatgagt gatgttatta
     2521 ccggattttt tattatcatg gaacaacaga tggatgttgg ggactacatc aagctagcaa
     2581 acttaacgat tgaaggaacc gtcgtatctg ttggtatccg tacccttcag ctgaaagcaa
     2641 cggatggtac tgtccatttc atcccgaatc gaaacatcac cacgatcagc aatctctctc
     2701 gtgcaaatat gcaagtactt ttagatattc ggattgttcc tgaagaagga tatgacaaaa
     2761 tctatgagat cattgatcga gtaaatcgta cattagctga aaaataccaa gaagagctcc
     2821 aaactgaacc ttctatcttt ggattagtgg atcttggaaa cagcaatttt gcgattcgta
     2881 cggtctgcta cgcattgaac ggaaaacaat atgccttaaa agaagagttc ttgagctcct
     2941 atgtcaaaga attgacagct tctggtttca cgattccgaa cagtccaatt gctgtcggaa
     3001 aataatttgg acaaacgatg caacgaatct cactaaaaag agcgtgggac aaaagtaaag
     3061 aacacttttg tcccacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3121 gaaataagct gaaattcaca aaaatttgaa aagc
//
LOCUS       DAZKAS010000209         3150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000209 DAZKAS010000000
VERSION     DAZKAS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.214"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..357
                     /locus_tag="ACG8H7_002711"
                     /pseudo
     CDS             106..357
                     /locus_tag="ACG8H7_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ACG8H7_002712"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ACG8H7_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP8984760.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ACG8H7_002713"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ACG8H7_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1786..1869
                     /locus_tag="ACG8H7_002714"
     CDS             1786..1869
                     /locus_tag="ACG8H7_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFP8984761.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACG8H7_002715"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACG8H7_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP8984762.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..>3150)
                     /locus_tag="ACG8H7_002716"
     CDS             complement(2994..>3150)
                     /locus_tag="ACG8H7_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HFP8984763.1"
                     /translation="KEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPK
                     TPKLPGI"
BASE COUNT         1036 a          502 c          631 g          981 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc
//
LOCUS       DAZKAS010000210         3117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000210 DAZKAS010000000
VERSION     DAZKAS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.215"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..1040)
                     /gene="murB"
                     /locus_tag="ACG8H7_002717"
     CDS             complement(105..1040)
                     /gene="murB"
                     /locus_tag="ACG8H7_002717"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP8984764.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1113..2033)
                     /locus_tag="ACG8H7_002718"
     CDS             complement(1113..2033)
                     /locus_tag="ACG8H7_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP8984765.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2146..2898
                     /locus_tag="ACG8H7_002719"
     CDS             2146..2898
                     /locus_tag="ACG8H7_002719"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP8984766.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          880 a          632 c          542 g         1063 t
ORIGIN      
        1 tgtgttaaaa tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac
       61 ggccaccgtc ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt
      121 ctgcttgttt ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac
      181 acatcaaatt tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc
      241 gctgtcgcat gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg
      301 ccccatttca ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact
      361 gggcgcttaa agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat
      421 tcggtcaact catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat
      481 cgagcttcca atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc
      541 atttcgtctt ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca
      601 aaaacatctt tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca
      661 cttcccggga tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa
      721 gtcgtatcaa tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc
      781 tgtttcattt cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga
      841 ttgcttgcat ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt
      901 tgctttactt cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc
      961 gtaaacgtat agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca
     1021 actatttctt ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc
     1081 tatcacgctt aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta
     1141 agcacttcct cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt
     1201 tccgatttac ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt
     1261 ttgactagcg tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcctcttga
     1321 atgacaagct gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat
     1381 ttttctatat tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt
     1441 tctaattgta aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt
     1501 ctcaaaatgg gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca
     1561 taaaggacga actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac
     1621 cgcttatctg ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg
     1681 atcatcaaaa ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga
     1741 tggacactca gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct
     1801 aattcataat gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa
     1861 agctcatcga tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt
     1921 gtttctttat ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt
     1981 ggtaaatatg ctttttctgc gatattccct acaccgatta caccaatctt catctacttt
     2041 cctcctggat catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat
     2101 agtatcgcaa aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct
     2161 tggaacgtaa acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag
     2221 ttagacgccg attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta
     2281 gatcttccag gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca
     2341 gcgatttttg ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat
     2401 gatacagaag gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac
     2461 acgccaaatt ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc
     2521 ttttataatt atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta
     2581 aatgtcgctc atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga
     2641 ttcactccag aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca
     2701 ttccgttatt tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat
     2761 tcaaggaaaa acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag
     2821 ccaggactag ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta
     2881 gaattagatt tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa
     2941 attttgtgaa aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc
     3001 ttttgaacac tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac
     3061 agccaaacaa aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagat
//
LOCUS       DAZKAS010000211         3071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000211 DAZKAS010000000
VERSION     DAZKAS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.216"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1720
                     /gene="alsS"
                     /locus_tag="ACG8H7_002720"
     CDS             56..1720
                     /gene="alsS"
                     /locus_tag="ACG8H7_002720"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFP8984767.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1733..2443
                     /gene="budA"
                     /locus_tag="ACG8H7_002721"
     CDS             1733..2443
                     /gene="budA"
                     /locus_tag="ACG8H7_002721"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFP8984768.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2573..3070
                     /locus_tag="ACG8H7_002722"
     CDS             2573..3070
                     /locus_tag="ACG8H7_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFP8984769.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1029 a          510 c          687 g          845 t
ORIGIN      
        1 ttgtgattat ttctaactaa taattaaaaa taaagcataa aaaggacgat gttcaatggc
       61 tgaaaaaatg aaacaaggtt cgaaatctgt agtagaaagt ttgattaatc atcatgttga
      121 ttatgtattc ggtataccag gtgcaaaaat cgatggtgta tttaatgaat tagaggatca
      181 aggtcctgaa ttaatcgtga ctcgtcacga acaaaatgct gcatttatgg cacaagcaat
      241 cggacgtatt acaggagagc caggagtagt tatcgcaaca agtggtcctg gtgctagtaa
      301 cttagctaca ggtttggtaa cagcgacagc agaaggtgac ccagttttag caattgccgg
      361 acaagtaaaa cgaagcgatc tattgaagtt gacccatcaa agcatggata atgcagcact
      421 gttccagcca atcacaaaat acagtgcaga aatccaagat ccagaaacaa tttcagaagt
      481 gattgccaat gcttatcgta tggctaagag ttcaaaaaaa ggcgcgagct ttatcagtat
      541 cccgcaagat gtagtggatg cacctgtcca aggtaatgtc atcaagccat tgagcgatcc
      601 taaattaggt tctgcttctg cagatgatat ccgttaccta gctgaacgca ttagagaagc
      661 aaaactaccg gtactattag taggtatgcg aggatcaagt gaaaaagaaa cgttagccat
      721 caggcaatta gttgaaaaga cagcattgcc tgttgtagag acattccaag cagctggagt
      781 gatctcacgt gaattagaag cgcatttctt tggtcgtgtg ggtttattta gaaaccaacc
      841 aggagatatg cttttgaaac gaagtgatct agttattgca attggctatg atccaatcga
      901 atatgaagca agaaactgga atgcggaaaa agatgcgcgg atcattgtga tcgatgaagc
      961 gccagctgaa atcgatcctt ttatgcagcc tgaaagagaa ttgatcggtg atatctcagc
     1021 cactcttgat ttattgacag gttcattaga gcctcaacaa gtgtcagaag atgcaaaaga
     1081 atatttagca tctcttcaag caaaattaac ggagcgggac attgttcaat caaaaggaga
     1141 agcaggtatc ttgcatcctt tagaagttat taacacgctt cagtcaaaag taacagatga
     1201 tatgactgtt accgtagatg taggtagtca ttatatttgg atggcacgcc actttagaag
     1261 ttatgaacct cgtcatttgt tattcagtaa tggtatgcaa acgcttggtg ttgcattgcc
     1321 atgggcgatt tctgctgcat tggtgcgtcc aaatacacaa atcgtatcag tttccggcga
     1381 tgggggattt ttattctctg cccaggattt agaaacggcg gtacgtaaaa aattgaatat
     1441 cgttcatttg atttggaatg atggacacta caatatggta gagttccaag aaaaaatgaa
     1501 atatcagcgg gcgtcaggtg tagactttgg tccagttgat tttgtgaaat atgcagaagc
     1561 ttttggcgct aaaggaatcc gagctacaag tgtagaggaa ttagaaaaag ctttagaaga
     1621 aggatttgcg acagaaggac ccgtaattat tgatattccg attgattacc gtgataatga
     1681 gaaattggga gaaacaatct taccagatca attctattaa ggaagtgtcg acatgactga
     1741 aaaaatctta taccagcacg ggacattagg agctttgatg gccggcttga tggatggaac
     1801 agaaacgatt gctcatatat tagaaaaagg aactctggga ttgggaacat tgcatgggct
     1861 tgatggtgaa gtcatctttt tagatggcgt agcttatcaa ggaagatcag atggctcagt
     1921 tgcccaatta gatggaagtg aacttacccc ctatgcagca attacagatt ttacgccaga
     1981 tactagtttt tctgtctcaa aaactgcaga tggagaacaa ttgaaaaaag acatattagt
     2041 aagagaagcc ggggaaaacc tatttttggc agtgaagata actggattat tcaaaaatat
     2101 gcacatccgt atcatgccga agcaagaaaa accttacaga agactagcaa aaatttcaga
     2161 gtctcaacca gaattccaac aaagcaatat ccaaggaaca ttagtcgggt tctttacgcc
     2221 tgaattattc caaggagttg ctgcagcggg atttcacctg cattttatcg atgatacaca
     2281 tacatttggc ggacatgtaa tggattttga agtggctgaa ggaaaagttg agattagccg
     2341 catctcgtcc cttgtacagc attttccagt tgatgatccg acttttgttc acgaaacgat
     2401 tgattatgct gatttagcag cagaaatcca acaagcagaa tagaaaaaga cagcgagatg
     2461 cgttcaaggt gattaacctg acgtatctcg tttttttgag gaggaaacct agcgtttaca
     2521 aaaaaactga gctataatct tctccataga tcaaaaagga gcggttttta tcatgtggac
     2581 agaaaaacag aaaaaagatg catacgaaat tttattatta cagcaacaag gattattaga
     2641 agcagaagat aactggttag caaatttagc aaattcatct gcattgttga atgagacttt
     2701 gcctgagact gtctttgcag gatattattt attcgatggt aatgaattga ttttaggacc
     2761 gttccaaggg cgagtttctt gcacacgtat caagatggga aaaggagttt gtggagaatc
     2821 agctgaaaaa caagaaacat tgatcgttga tgatgtcaaa acccatgcaa attacatttc
     2881 ctgcgattca gccgcacaat cagaaatcgt agtgccaatg gtaaaagaag gtaatttaat
     2941 cggtgtttta gatatcgatt gcggagtaac gaaagggtat gatgaaatcg atcaagcatt
     3001 tctagaaaaa tatgtagatc ttttattagc aaattttaat ataaatgctt tttcatttat
     3061 taataactaa a
//
LOCUS       DAZKAS010000212         3039 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000212 DAZKAS010000000
VERSION     DAZKAS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.217"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(256..870)
                     /locus_tag="ACG8H7_002723"
     CDS             complement(256..870)
                     /locus_tag="ACG8H7_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP8984770.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1476..1853)
                     /locus_tag="ACG8H7_002724"
     CDS             complement(1476..1853)
                     /locus_tag="ACG8H7_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984771.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2153..3034)
                     /gene="galU"
                     /locus_tag="ACG8H7_002725"
     CDS             complement(2153..3034)
                     /gene="galU"
                     /locus_tag="ACG8H7_002725"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP8984772.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          638 c          468 g         1015 t
ORIGIN      
        1 tctttttcca ctacttttta tttttctgac actacatatt cttataactc atttctcata
       61 actgtgaaac aacttataaa aataacaaac tttaaacaaa acaagcttaa aatcaaagat
      121 gatccatctt aaaataacta aaacaaaaaa agtagtcgtt gttaatcctc attaataatc
      181 gttttctttt tttcaaacgc tccattttta tgtggaacat tattttttta aggaactttg
      241 tcaacatgtg gataactatt ttccacttgt gtcctggact agctgttttg ctgtaaaata
      301 caaaacttgc tgcagcaaaa agatcgttgc aagagaggct gttccgcaga tcgctggatc
      361 tagatttggc aaagtcaaga ctgcatcatg taaattggcg tggttgtcca aaaccaaatc
      421 agcaaattca aataatcgca aaccactgct atgttgcgat ttgatacttc gtgacaaatc
      481 aaatccagta acaatgatta atggatgacc attttctttg atccactgag ctagttcgat
      541 gattgccggg tttcttcctt cattcgaaag aagaagaaaa atgtcttctg atttgacaga
      601 aagctgatca ataattgcct gtcctgcacc ttctagcgtt tcatagacac ctccggctgg
      661 attttcaatc aatatacttg gtacaagatc cggggtttga tcccaaaatt caaatgctac
      721 accacttagt gtgcggctag catatatctg caatctacca ccttgattaa tttgtttcgc
      781 gatccaagtg ccagcttgaa gcatcagcgg ctcttctatc cttaatttct cttccaattg
      841 gtgataatag tcaaaataac ataaactcat ttatctctcc cgcctagcta tacttgacat
      901 gaatagaaac agctgcctaa tctaaggaag tacataaaaa aacagtagtt agacagtaac
      961 acatgtggtc tttgctgttt caaacacaaa aaaggcattg aacgaatacg cccaatacct
     1021 tgacatagca aataaaacaa cgtgcagacc atggaaaata tctacatagg ggtagaattt
     1081 tcaccctaga ttaggtacaa taaattaggt cgatgcactg cattgactat ggcaaaatga
     1141 tcggtcaggg tgtatgtgtg tgactggtca ttccctgatt cttagcgcca actaagggtc
     1201 agggagcctt tttttattca gctgtttcta tctgaattta ttgtaagtaa tctcttatcc
     1261 aagtgcaagc aagaaaatac aaaaaaacat tggtatgatt tatatatata taaataacat
     1321 aaaaaaagga atggcttatc ccgatacaac aagcattcct ttaaaaaaga ttcttttata
     1381 taagtgaaaa aagctttttt ccaccaatta aataaatctc tcatctcaat agtttatcca
     1441 ttattttatc tttttttgcg tatacctact aaatattacc gtcttcttct cgttcttcta
     1501 ctttgttcga gagaatctgt actagaaaaa gtagttgtat gtctaccttt tctgcctatc
     1561 cgagactttc ttttcatata tgggatataa atgattagat acagtacagc aaaaaataaa
     1621 gcaaatacgc aagcttccca actacctaac gccaattcaa ccatcaaggt agcaacccca
     1681 acggatgctg cataccagat aaaaatcaga aacgtttctt tttttctgac catgcgaaac
     1741 atgataaata gactaataag caatagtggc aacacaaata tccaattgct tccttccatc
     1801 agccacttga gtatatcaag cagcgactgt gcaacagcga ctaattttcc catagtgaac
     1861 acacaccttt ttttataatt aaaaaattcg cctttttatt ctactatgaa tcttctggct
     1921 tggctttcat ttctcttatg taatcatttt cttttataca acttaaaaaa aaattaaacc
     1981 attagcataa tattttctga atctattcac gaaagtcttt ttctatcttt cgttttccac
     2041 gactcaactg atctagagtc aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct
     2101 gccgctttaa taacaaatag cctttcattc tcagcaggcc acttatttcc agttaacttt
     2161 ctgttcttag ctttttactc aactggataa tatatgcact taatgaagtc ccaatttccg
     2221 aatgttgaag tccatattca atatttgtct tcatatatcc taacttatcg cctacgtcgt
     2281 atctttttcc tttaaactct ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg
     2341 catccgttag ctgtatctcg tttcctgctc ctggttcttg agtttctaat atctcaaata
     2401 ttttaggagt cagtagataa cgtccgataa tggctaaatt acttggtgct tttcccattt
     2461 caggtttttc tacaaaccct tttactctgc aacgaccctt ttcgctatct gttttaggat
     2521 caataattcc gtatttagat gtttcatttt ctggtacacg catgacggca acagcggaag
     2581 cttgtgtttc ttcataatcc atcatcaatt gttgagtcaa tgggatatca tctttcatca
     2641 aatcatcccc cagcatgacg acaaacggtt catttcctat aaatgctttt gcttgaagca
     2701 cggcatcccc cagtcctctt ggatgagatt gacggatgaa gtgaagattg acttgagtcg
     2761 tttcttcaac taaagcgagt aattctgttt tttccttttc ccgcaaattg ttttccaatt
     2821 cgatatttgc atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga
     2881 tgtcttcgat tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt ggtttatcca
     2941 cgattggaag catttctttt gccatggctt ttgttgccgg caaaaaacgt gttcctagcc
     3001 cagcggcagg aatgactgct tttttgacct tcacttgct
//
LOCUS       DAZKAS010000213         3013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000213 DAZKAS010000000
VERSION     DAZKAS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.218"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            177..359
                     /locus_tag="ACG8H7_002726"
     CDS             177..359
                     /locus_tag="ACG8H7_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFP8984773.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="ACG8H7_002727"
     CDS             462..746
                     /locus_tag="ACG8H7_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984774.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="ACG8H7_002728"
     CDS             762..998
                     /locus_tag="ACG8H7_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984775.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="ACG8H7_002729"
     CDS             1020..1274
                     /locus_tag="ACG8H7_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984776.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="ACG8H7_002730"
     CDS             1356..1541
                     /locus_tag="ACG8H7_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP8984777.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="ACG8H7_002731"
     CDS             1608..2003
                     /locus_tag="ACG8H7_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP8984778.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            2020..2370
                     /locus_tag="ACG8H7_002732"
     CDS             2020..2370
                     /locus_tag="ACG8H7_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984779.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="ACG8H7_002733"
     CDS             2392..2766
                     /locus_tag="ACG8H7_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984780.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DAZKAS010000214         2975 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000214 DAZKAS010000000
VERSION     DAZKAS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.219"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACG8H7_002734"
     CDS             complement(<1..1097)
                     /locus_tag="ACG8H7_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP8984781.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ACG8H7_002735"
     CDS             complement(1227..2603)
                     /locus_tag="ACG8H7_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8984782.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..>2975)
                     /locus_tag="ACG8H7_002736"
     CDS             complement(2603..>2975)
                     /locus_tag="ACG8H7_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8984783.1"
                     /translation="SPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVA
                     IARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSAD
                     IILELSGKKLKKVNKMNLEVE"
BASE COUNT          904 a          568 c          415 g         1088 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgat
//
LOCUS       DAZKAS010000215         2964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000215 DAZKAS010000000
VERSION     DAZKAS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.220"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8H7_002737"
     CDS             complement(37..1257)
                     /locus_tag="ACG8H7_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFP8984784.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8H7_002738"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8H7_002738"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP8984785.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8H7_002739"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8H7_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP8984786.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          810 a          620 c          515 g         1019 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgt
//
LOCUS       DAZKAS010000216         2909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000216 DAZKAS010000000
VERSION     DAZKAS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.221"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..1242)
                     /locus_tag="ACG8H7_002740"
     CDS             complement(163..1242)
                     /locus_tag="ACG8H7_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8984787.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            1386..2354
                     /locus_tag="ACG8H7_002741"
     CDS             1386..2354
                     /locus_tag="ACG8H7_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFP8984788.1"
                     /translation="MNATRRIAKITSWKEAFATLQAKIDIQVMNRNGYNESKKQKEHL
                     LKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNCIWVCWWQGLDQSPEIVKA
                     CVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGIITRTNYSDLLRLSLLAKH
                     GGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASGYFAGYSLACNQESRWMFA
                     IIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTFDKIPSNNPLCDELIKNLN
                     NIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGMLVENKLL"
     gene            2376..>2909
                     /locus_tag="ACG8H7_002742"
     CDS             2376..>2909
                     /locus_tag="ACG8H7_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP8984789.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKN"
BASE COUNT          875 a          534 c          665 g          835 t
ORIGIN      
        1 catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg agtgggccct
       61 tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag ctttccagac
      121 acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg tcaaccgttc
      181 ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa tctttcgcgg
      241 gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt tgccgattgg
      301 gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg tcccgcgctc
      361 ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg catcgtgcag
      421 tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc cgaagtacgc
      481 accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat ccttcccggc
      541 cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca gcacggcatc
      601 cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg ttttccgggt
      661 accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat tcgggcggtt
      721 gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca tattcttcgt
      781 tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga taatggccgg
      841 gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc atttttccaa
      901 cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata agggccgtct
      961 gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt cgtagacgtc
     1021 atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag taacggtccc
     1081 gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg caacctgacg
     1141 gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt aggttaggtg
     1201 ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc atccttgtct
     1261 attgagagtt gtggtaactt caatataaca tggaattcac gtggcgtttt tttatttcca
     1321 aatggctaag ttaattataa aatccgccaa gtataaaaag aattatacat cgcatatccg
     1381 aagaattgaa tgcaacaaga cgaattgcaa agataacatc ttggaaggaa gcatttgcca
     1441 cgttacaagc taaaattgat attcaagtca tgaatcgtaa tggatataat gaaagtaaaa
     1501 aacagaagga acatttatta aaaaaacatg aggtaatgct gaagtattat gaaaagactt
     1561 tttcagattt tttgaaaaca tatgattata gtcaaaatca aataattaat caatcaaaat
     1621 caccatattc taactgcatt tgggtttgtt ggtggcaggg attggatcaa tcgccggaaa
     1681 ttgtaaaagc ttgtgtaaaa tcaataaaac gaaatgcagg gaaacatact gttataattt
     1741 taacagaaga taattataga caatatgttg atataccaaa atgggtggaa gaaaagaaaa
     1801 acaaaggaat cattacgaga acaaattatt ctgatttatt aagattatct ttgctagcaa
     1861 aacatggagg tttgtggtta gacgccacat tttattgtac aaaaccaata ccagatgagt
     1921 attttgatat cgatatttgg tctattagga ggcctgatta tgctcacgca tccgtagcat
     1981 caggatattt tgcgggttat tcattagcat gtaatcaaga gtctagatgg atgtttgcta
     2041 taattagaga ctttttttta aattattgga aaaataatga tatgatggtg gattatttga
     2101 tgatagatta tatgattgtt ttagcacaga gaataaatca tgaaatagca cagacatttg
     2161 ataaaatacc tagtaacaat ccattatgtg acgaattgat taaaaattta aataatatat
     2221 atgatgaaaa agtatggaac ttattaaaga aagatacttt actgtttaag ttgacatgga
     2281 aacaatcttt taaaaagcaa aaagatgata aagttacttt ttatgggatg ttggtagaaa
     2341 acaaattatt ataaatatta gaggtataaa aagttatgaa acacaaggta agtgtgatag
     2401 taccaattta caatgttgaa aaatatttaa gaaaatgtgt ggatagttta ttgagtcaga
     2461 cattaaaaga gatagaaatc gtattagttg atgatggttc gccagatgct tcgggggaaa
     2521 tagcagatga gtatcaaaaa aaatattcta atgtaaaaac aatacataga gaaaatgggg
     2581 ggttaggacc ggcaagaaat acaggaattg agaatgcgac aggagaatat gtggcttttc
     2641 ttgattcaga tgattgggta gaaagtgata tgtatgaaaa attatactta gttgcgtcca
     2701 aagaaaatgc tgatatagta gttagtggac attgtgattt tgcgaatgac aaagctatgg
     2761 tgaaaaagca acatcctttg agtgggaatg tatatgatac gcctaatagt attaaaaatg
     2821 tacgaaaaaa tttatttgga catagtccaa aagatcaaga agtagaggcc ttcccgatgt
     2881 cagtatgtat gtctttgtat aataaaaat
//
LOCUS       DAZKAS010000217         2886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000217 DAZKAS010000000
VERSION     DAZKAS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.222"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            798..1052
                     /locus_tag="ACG8H7_002743"
                     /pseudo
     CDS             798..1052
                     /locus_tag="ACG8H7_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="ACG8H7_002744"
     CDS             1122..1634
                     /locus_tag="ACG8H7_002744"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8984790.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="ACG8H7_002745"
     CDS             1631..2668
                     /locus_tag="ACG8H7_002745"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8984791.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1041 a          513 c          574 g          758 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacaccgac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
     2881 ctctac
//
LOCUS       DAZKAS010000218         2852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000218 DAZKAS010000000
VERSION     DAZKAS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.223"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..247
                     /locus_tag="ACG8H7_002746"
     CDS             29..247
                     /locus_tag="ACG8H7_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984792.1"
                     /translation="MDLYDIESGATVSVESDHLIISFADERQIIIWVVDDMLYPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            244..642
                     /locus_tag="ACG8H7_002747"
     CDS             244..642
                     /locus_tag="ACG8H7_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFP8984793.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            639..977
                     /locus_tag="ACG8H7_002748"
     CDS             639..977
                     /locus_tag="ACG8H7_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984794.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            974..1204
                     /locus_tag="ACG8H7_002749"
     CDS             974..1204
                     /locus_tag="ACG8H7_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984795.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            1211..1414
                     /locus_tag="ACG8H7_002750"
     CDS             1211..1414
                     /locus_tag="ACG8H7_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFP8984796.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            1411..1716
                     /locus_tag="ACG8H7_002751"
     CDS             1411..1716
                     /locus_tag="ACG8H7_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984797.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            1700..1993
                     /locus_tag="ACG8H7_002752"
     CDS             1700..1993
                     /locus_tag="ACG8H7_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984798.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            2253..2669
                     /locus_tag="ACG8H7_002753"
     CDS             2253..2669
                     /locus_tag="ACG8H7_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFP8984799.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         1044 a          392 c          656 g          760 t
ORIGIN      
        1 aatgataaaa aagctcattc aattcagcat ggatttatat gatatcgaat caggagcaac
       61 agtatctgtg gagtctgatc atttaatcat aagttttgct gacgaacgtc aaattatcat
      121 atgggtagtt gacgatatgc tatatccaga aattgtacat gattttgaag aatcaaaagc
      181 ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag
      241 caaatgatac cgaagtttag agcgtgggat aagcgagaaa acacaatgag agatgtagct
      301 gtcttacatt ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc
      361 gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat
      421 aagaatggtg tggagatatt tgaaggggat gtagtaatta catcaaaagg agcgattgga
      481 tatgttgcgt atcttcaaca agaagccggc tttgtagttg tactaaagaa aagtgattat
      541 agattaggcc ataggaatac tggcgaaagt tatgatgtag caactgctca tgaagtcatc
      601 ggaaatatat acgagaatag cgagttattg gaggtagagt gattatctta gctactgact
      661 acatcgattc tttaaaagat gaaaatggga atgtcccagc attgctaaat tgtggaatta
      721 aaagcaagaa tagaatcatt ttgttacgga tatcggctga tttagcaaaa tatctttatc
      781 tgagcaagac agcagtatat tacactgcgg ctacacgtta tcagtacaag ggaaaaacaa
      841 tcagtcagga ttattatgat cgtttaatca gtcctgacat gcatggaaaa tcaaaatcag
      901 cgatcaagtt atttggcgca atagagatat atgccgatga ttttccaaat ttatggttga
      961 aggaggaagc ggaatgaaat acgaaatacc actaagtgaa gcgggcattc aagcaattat
     1021 caatggtcgg gaggttaata aaaaacttcc tgatggtact gaattagtca tcagacaaag
     1081 ttatttgaaa gatatggcag ctccagtatt aattgatcgt tttaacgtga ctgattctgt
     1141 ggtagagaac cacttgaaag aatttcgatc aagtataaac gacactttca gattagggag
     1201 ttgattgaca atgaacacca gacaccgcag agtagcaaaa ctaagaaaac aggaactgaa
     1261 tgtactaaag acaaagtttg aaaaagaata tggaatttca gcagaagaaa catataaagt
     1321 ggcaagtcag tttgttgctg atgcgagtga gactattcgt aagtttggga tttcgatatt
     1381 aaatgatgat cgtaaatggg aggcagaaag atgaaactaa aagacggatt ttacactgat
     1441 gcttatggaa ttggtggttt gatgatggat ctaccaacta agaatcctat aaagcaagag
     1501 gaatcagaaa ttaaagtcgg tgacatggtt cgctgcgaag cagaagggtt catctatcca
     1561 tttcgtggat atgtagagca tctctataat cactcagcga tcattcgtat tgaaaacaca
     1621 atggaatgcg ataagtggtt agcgaaaagc aaagagaatt tagcagtggt gagattggtg
     1681 gatatggagg ttataaacaa tggaattaaa aattgacaag gtaacgtttg aagaaccagc
     1741 atttcaagaa atggatgaac gaattgcaga attggtcgag aaaaaacaag atgaattgat
     1801 tgctttgcta gaaaagaaat accaatttcc gaaatatatg aacaaaatgc aagcagcaaa
     1861 atatcttggt gtaagttaca acacgatgat ggataagtac gtaccagctg gtttgaaaat
     1921 gggtatcgtt aacggcgtta ttcgtatcag caaagaagaa tgtgatcgtt ttatgaaaga
     1981 acatcaaaaa taaaataaaa aaacgtttag ccccttagct aaacgcacaa ttataattta
     2041 cctgacagtt aattataaca aaattgcgga ggttgaggaa tggcggaatg aaaaagacaa
     2101 taaaaacaga agaagcatat attgaagtat ttgacacacc agcatgctaa gtcgagaagt
     2161 gtaactcggc aaataaaaaa agccggatcg ctccgactga tgtaataaac ccaacaagtt
     2221 tattatatca cataaaggag cggtttgact tgatgcaatt gttacgagag gtagatttca
     2281 aacagacaag atgcaatgcg agagatgtgc tgaagaattt tcgacgctta gagcggatgg
     2341 caggtcgctc tttgatagat attaagtcgc cgattattac ggatatgccg aaggcaccga
     2401 agcacggcaa taaggcagag gacgcgatta ttcagatgat ggatatagaa gcagagagag
     2461 atgcgattct agcggctttg atggctctta gtctgattag ccgtcagata ctctattata
     2521 gcttttgtga cgtaaacaag cactctaatt atgaaatagg gcaattgata cgaggatacg
     2581 gagagaagaa tgtagagaag ctgaaatcta tcgcgctgat cgaatttgct gaagcataca
     2641 aaaaaggcgt attagttcag tatcgttgat tttgtagggt ttttgtaggg atagtgtagg
     2701 gtttttgagt ggtttaacgt gatattatga tagtgtcgaa agattagtga taggtctaag
     2761 acaaaataat aataaaagga acatcgtttt attattgttt cacaattaag cttcgataga
     2821 cagcagcgga aatattaaga ataaggatgt ga
//
LOCUS       DAZKAS010000219         2805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000219 DAZKAS010000000
VERSION     DAZKAS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.224"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..572
                     /locus_tag="ACG8H7_002754"
     CDS             384..572
                     /locus_tag="ACG8H7_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP8984800.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="ACG8H7_002755"
     CDS             650..1663
                     /locus_tag="ACG8H7_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP8984801.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="ACG8H7_002756"
     CDS             complement(1730..2764)
                     /locus_tag="ACG8H7_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP8984802.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT          871 a          536 c          546 g          852 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgaca
//
LOCUS       DAZKAS010000220         2707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000220 DAZKAS010000000
VERSION     DAZKAS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.225"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(88..1035)
                     /locus_tag="ACG8H7_002757"
     CDS             complement(88..1035)
                     /locus_tag="ACG8H7_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP8984803.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1040..1792)
                     /locus_tag="ACG8H7_002758"
     CDS             complement(1040..1792)
                     /locus_tag="ACG8H7_002758"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFP8984804.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1793..2701)
                     /locus_tag="ACG8H7_002759"
     CDS             complement(1793..2701)
                     /locus_tag="ACG8H7_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFP8984805.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
BASE COUNT          883 a          512 c          338 g          974 t
ORIGIN      
        1 ttctatccca gatttgattt tacaaaactc atcagtcctt attgacaaag agcctgaatt
       61 taccctctaa tcttatctta gtatgtttta attgatttca gttttcaaat aattagtcat
      121 tccacttaaa ataccatatt tcggttgata gccaattgac tggatcttag taatatctgc
      181 tagagagtca tggatatcac ccgaacgctc ttctttgtat tcaagtgtca attctacacc
      241 gagaatttca ttgattgcat gaatcaattc caaaagtgtg gttgctttac ccgttccaac
      301 attgaattgt ttccccagtg cagcttcctc attcgcaact aaaagtaatg cttggattac
      361 atcatctata taaacaaagt ctctagactg gcttccatca ccaaataatg taaatgaagt
      421 tgcttctcct acgatttgtt tcttatagcg atctacgaga atagaaatca caccactgta
      481 tggagaattc ggattttgat ttggaccata gacattgaaa aaacgaactg cgctaccggg
      541 tacatcatat aaatgacaat aatctagcac atattgttct gcagcgaatt tatcaaccgc
      601 ataaggcgtc aatgggcgaa tcacggactc ttcttttttt ggtaaagtcg gttcatcccc
      661 ataaacagcc gctgaagaac taaacacgat ccgttttaaa tcaggttgat acttgcggat
      721 caattctaaa atcattaata cactttcaaa attcacacga tgtgtttcta ctggtcgggc
      781 aactgaatct gccacactag caactgctgc taaatgaaag atataatcaa atttattagc
      841 tttcatgatc tcttccatca aggatttctc agcgacatct ccctcaataa aagtgatatt
      901 tttcgtcata tcaagatttt ctacttttcc cattgataag tcatcgacga cgattaccac
      961 atgttcttct cctaaatgat tggctaaggt agaaccaatg aagccagccc caccggtgat
     1021 aagataacag ctcatccttt tacctccgat ataaataatt atagatatct tgaagctccg
     1081 aagaatcttc taaaaattta ggaaacaaaa attctaacca tttaaaatct tcttcactat
     1141 caatatctaa aattagataa tctctcattt ttattatccc acattgccca tcaaaaatat
     1201 aattactttt ttttagaaat tcaggtttaa aagaatacat tgaggcattc atttcaaaaa
     1261 cttcaggtgt ctgttgccta gatgtgtatc ttgatttatt aactatagta acttcatttt
     1321 caattctttt taccatatta aaatagggat tacgtcttgc agaaacaact gaaaagtaga
     1381 cgtctttatc taaatttttt agatgttcat ctattaattc ttcaagatta gaaacttcac
     1441 gtaaaggtga agtcaaatct aaatctataa ttaaatcaaa tatattatct tctctttcta
     1501 gttgtaagaa tgaatcacgt atcacttcaa ttttccctac agtatctgta gctaattcag
     1561 gtttacgttc tacaattttc acagacggat ttaacttagt cgtcaattct actaactcat
     1621 tactgtccgt attcaaacaa gtcgtgcaat tatgttcagg atgtttttgt tgatagatat
     1681 ctatcattgc taaagcgaaa tggactaggg gctttcctgc caaatatttc aaatttttat
     1741 ttttaaaccc cttagaccca gctctaccac aaatagtaaa taaaatttcc atttatttct
     1801 cctcagctat tttcaatact tcgtttgcat gatataaatc attccagttt tctttgcctg
     1861 ttactacgtt ttcaaagaaa tatttcaact ccttctcata catatcatta gcagattcag
     1921 gcatttcttt aacaatgttt tcttcaaaac ttattttatt atttaataag tcaacattga
     1981 tacattcatc taaagtaaac aattgaaatt ttcttgtagg aactcttcca aaataatcta
     2041 aatgaatttc tataatttta tcatcgtatc tcaatatata tgttgctaaa tcattactgt
     2101 taatttcaag attcgaatat ttaccaaact gtttttttac tgatttaggg aaaccaaata
     2161 ggtttataat ataatccaat tcatgtatac agtctaattc tactcctccc cccatagatt
     2221 taaaggcaga ataattattt ctataatcag taccttttct ccaatcagga agataagtag
     2281 aacaaatagc tcttacagaa tatacttttt tactttgaat actattacga acaacttcca
     2341 tgatattttt atacctcagt ggagcagcaa cataaatatt tttgtaatca taattatatt
     2401 gagtaagatc aatactctca gaaaaagcag gtttttctaa aaatatgtaa tcaacttttt
     2461 caacaattac atttagtgtt tccagatgct taaaagtcgg atttgtaata aaagctatat
     2521 cataatgtgt atccaaatcg tctctatttt gaatatacgt tatttcttca aactcttttt
     2581 ttccactgct actataagct gttatatcaa aattaataga attctttttt ccatagctta
     2641 ttagattttt tagatgtctc tttccaattg accctaaccc aaaaaaaatt actttcatca
     2701 ttaattt
//
LOCUS       DAZKAS010000221         2655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000221 DAZKAS010000000
VERSION     DAZKAS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.226"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..281
                     /locus_tag="ACG8H7_002760"
     CDS             45..281
                     /locus_tag="ACG8H7_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP8984806.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            300..515
                     /locus_tag="ACG8H7_002761"
     CDS             300..515
                     /locus_tag="ACG8H7_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFP8984807.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1218..1970)
                     /locus_tag="ACG8H7_002762"
     CDS             complement(1218..1970)
                     /locus_tag="ACG8H7_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8984808.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1985..>2655)
                     /locus_tag="ACG8H7_002763"
     CDS             complement(1985..>2655)
                     /locus_tag="ACG8H7_002763"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8984809.1"
                     /translation="QYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKE
                     IANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKI
                     CCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISI
                     SNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQV
                     ILLF"
BASE COUNT          850 a          419 c          429 g          957 t
ORIGIN      
        1 acgtatgtta cagtagcacc gtaaaaaata ttatttaaag aagcgtgttt aaacatgcaa
       61 aatgtaaagg aactaagtat tgtggaaatg cacaaactgt tggtggaggc aataatgctt
      121 ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg ggcagtaggt ggtgcaggtt
      181 taggaacagc aatttgcgga ccagcttgcg gtgttgttgg agggttatat ggagctgtcg
      241 ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata aaaaaggaga ataagtagta
      301 tgcatattaa aaacacaaaa acaacattta tattatcttc agaagagttg aaaaatattc
      361 aaggtgggag tgccgtggga gttttaggca ctactttttc tggggcaaca gcaggagtaa
      421 aactttgttc agccggagga ccttatgcaa tagctgcttg tggtgtaggt ggagctttat
      481 taggtgctgg attcagcatg tggaccggtg cgtaaacaat taaaaaatat caaacctcaa
      541 gattggtttg tgtcgattac gaacataggt atgttcttta tcttttcaaa agtttggaat
      601 ggacagaact ttttactaaa gcttcttgtg ctagttttta taattatatt aaatttttat
      661 cttttattaa agtcagataa aaattctttg gaaatgtaat ggtagagata tttttacaaa
      721 aggacttgca gcgctgtagt tggtgctgca ggaggaccta tttcggccgt tacaggaggt
      781 attggtaatg tacttgtcgg atgtgcatta aagcctgttc ctgcaagtta aatttataaa
      841 aacaggaaag taattatctc aaatcctatc aagtacaatc ataggattgc acttgacagg
      901 acaaattttt taactaaaaa atgaaaaaca ctttctaaaa attagtagaa tttgccaaat
      961 ctttcgcatc ttccaccata catttaattt taattcccct ttatggtact taataaaaac
     1021 aagaaaagaa ctattcattt ggcccttttt tggttccaga ccatagaaat attcctccag
     1081 taactaatat tgctgttatg cacgtaacta ataacagcta taaaaattgg tacaacttta
     1141 ataggattca gaaaaatttg cactagtgaa ggaatctatt ttattagaaa aatgcaatct
     1201 ccctaaagaa aaaaccacta aacagtgctt tctttcaact tctccttaat tttttgtgct
     1261 ttccctaaag taaagtttct ggccaactct gaatcaaaat atagtgaacg ttttttaaaa
     1321 tctatttctt taacgttaaa gatatttact aaacacgctc gactaattct tgttaacata
     1381 ggatatttct tttcaagctc actaattctt ccataaaatt cgtactgccc atctttagta
     1441 cacaatgata atctatgtgg taattttgaa gcttctacaa aataaatgtc atttatatca
     1501 aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt gattcgtaac ctgttttgtt
     1561 gcatctatcc tttcttgcgc taataacaat aactcaacaa tctcgtttcg atactcatct
     1621 agtgtttgat cttttgttac aaatcctaac gtttcaactc ttctttttat tgttacgggt
     1681 aacatctcat catgagtagt tgtaaaaatg atttttgctt gaacatcata ttttctgatt
     1741 acttctgcta attctatacc gttaacttca tgatttaaat caatatctaa gaaatatatt
     1801 ccttgctttg gtcggaattg tttgaggtat tttttaactt ctaacggact ttgtgttttc
     1861 aatactatct taaatacttt atcgtgaaaa agaatgaaat tatcaataat tcgttctaac
     1921 tgatttaatt gtataatttg atcttcacat aatattattg gataactcat aaatcctcct
     1981 tccctcaaaa caacaaaata acttgagtag taaattgata cggatcgttt tgatagttca
     2041 caaacatatt gggattcttt ttattgattt cctctatact agataaacct aacccttgat
     2101 gtcccttttt tgttgttatt ccttttttca ttagagtatc tatagagagt agtatctctc
     2161 tacaactatt cgatatcgaa atttctatct gtttatcatc tcggtaaata gctagagaca
     2221 gttctctatc ttgactctct atagatgctt caattgcatt atcgagaata attccaagca
     2281 ccctaataca gtcaaatatt ggaacaggaa cttcaataat agtacttgga cattcgaatt
     2341 tacaacaaat cttattgtta tttgcttggt atagcttcgc tatcaacaaa cttttcaaat
     2401 attcattttt tatatttttt aaataacgat attcaaactt attggttaga taagaattag
     2461 aatagatctc taattctttt atttcttttt ccaatatcga atcatggttt tgattagcga
     2521 tttcttttaa actcagcaat aaatttttat agtcatgtct aaatctggca aatttttctt
     2581 gatctttttc cagtgaatca gtatattttt ttagataaac caattctttt cttttcaatt
     2641 gctgttcata ttgtt
//
LOCUS       DAZKAS010000222         2648 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000222 DAZKAS010000000
VERSION     DAZKAS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.228"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..303
                     /locus_tag="ACG8H7_002764"
     CDS             <1..303
                     /locus_tag="ACG8H7_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP8984810.1"
                     /translation="GQFQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSY
                     YVSTRTSRSKSESEANCFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            353..1006
                     /locus_tag="ACG8H7_002765"
     CDS             353..1006
                     /locus_tag="ACG8H7_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984811.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETNISKTKET
                     TEISSSTSKSTSKTDSSSTVTSSNQATAKPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNTEAQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            1143..1793
                     /locus_tag="ACG8H7_002766"
     CDS             1143..1793
                     /locus_tag="ACG8H7_002766"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFP8984812.1"
                     /translation="MKKNLILGFICTSILTLGACSSPSNNATNSETQKTINSLKKENE
                     ELKKQIASQSNDTEENSESIDNASKEKTLPVVGILEYTISSIKTEQVTNDEDNFTNAE
                     YNFSGIENFPEKYYRTTISYKLKNKATSPFSLSSYQASLIDGDGMEYSRTAGEYFLYD
                     ENSNSLVQPETTTSGVFYLLSKNKPNLSTFKLNASKQFTDDFTAEPIGEAGTTEYK"
     gene            1903..>2648
                     /locus_tag="ACG8H7_002767"
     CDS             1903..>2648
                     /locus_tag="ACG8H7_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8984813.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRK"
BASE COUNT         1036 a          452 c          448 g          712 t
ORIGIN      
        1 gggcagtttc aagaaattag agatcgtgcg ataatctttt taaatgatga actgcgagac
       61 tctgaggaaa gattctacat ttgcgctcac gaattaggtc acgctatttt tcatcgtggc
      121 ttatccagct attatgtatc aacaagaaca tctagaagca aatctgaaag cgaagctaat
      181 tgctttgctg ctaatctcat tgtttctctt tataaagaag ataatgatca atatcctaaa
      241 aaaatcgagg aattaaagaa tctttacggt cttccagaaa gcgcttatcg ttttcttata
      301 taaaaaagcc cgtgttagca cacatattac aacgagaaag aggaattata aaatgaaaaa
      361 agttagcgtt atgttgttgt taagtactgc tctgctactt tcagcttgtt caaataataa
      421 aaaagctgaa tcaacagatg ccacttctaa ccaagaaaca aatataagta aaacaaaaga
      481 aacaactgaa atcagttcat ctactagcaa atctacatct aaaacagatt ctagttcaac
      541 agttacaagc tccaaccaag ctacggcgaa acctagccca acagttataa gctccagtca
      601 ggatacaatc caaaccgcac ctcaagaaga aacatatgaa cagatgaaac aacgcacttt
      661 acagtcaact ccagctgatc gtgcaaattg gtccaacaaa gagtgggaag ctttcggcgt
      721 ggccctttat gaaaatggat tgactacaga tgatgctggc aatattatca gtcaagatca
      781 gaaagaacaa caagctgcat ctcaacaaaa tacagaagcc caacaaacaa gcgctcaaca
      841 agacgctgac actttatcac ttactgattt tgttaacaaa tacgggatgt cgcctgttgc
      901 atggaaagta cagaatggaa tgtctgaaga agaagcattg cgtacaacac agcaaaaaac
      961 ttccggtgaa gttcaattag gattttctaa atacggaata caataatata tttttgtgtc
     1021 ccactatctt gcctataacc tctaaaaagt tataaagaaa aaagcccgtg ctgcaacacg
     1081 gactaaaatc tcatttctaa gattctacat aaaaataata tcatagaagt gaggaattaa
     1141 aaatgaaaaa aaacttaata ttaggattta tttgtactag tattttaact ttgggtgcat
     1201 gtagttcgcc ttcaaataat gcaactaata gtgaaactca aaaaacaatc aatagtttaa
     1261 aaaaagaaaa tgaagagctt aaaaaacaaa tagcatcgca gtcaaatgac actgaagaaa
     1321 atagcgaatc tatcgacaat gcttctaagg aaaaaacctt gccagttgtt ggtatcctag
     1381 aatatacaat ttcttctata aaaactgaac aggtaacaaa tgacgaagat aattttacaa
     1441 atgccgaata taactttagt ggaatagaaa attttccaga aaaatactat cgtactacta
     1501 tttcttacaa gttaaaaaat aaagcaacat cacctttctc tttgtcttcg tatcaggcat
     1561 ctttaataga tggagacggt atggaatata gtagaacagc tggagaatat ttcttatatg
     1621 acgaaaattc taattcttta gttcaacctg aaacgacaac atctggggtt ttctatttac
     1681 tttctaaaaa taaaccaaat ttaagtacat ttaaacttaa tgcgtctaaa caatttactg
     1741 atgattttac agctgagccg attggcgaag caggaacaac agagtataaa taaaaaacac
     1801 gccccctccc tcgccaaagt ttgtggacgc gttaaaaaca aacctacaca ataggcttct
     1861 ttacagtgcc tattgtatca gagaaagaga gtagattcaa ttatggcaag attagtcaaa
     1921 cgtggaaata gttggcaata cgaaatttca tacaaaaaag atgacggaaa atacacgaag
     1981 ataagaaaat caggattcaa gacaaaaggc gaagcaaaag atgccgccaa cgaattagaa
     2041 tataacctga ataaaggtct taaaggggat cgcaaaaatc tattattatc agattacttt
     2101 gaagattgga tgcaactcta caaagaagga acagtatctc ctatcactta tagaaaatac
     2161 gaagatacgt taatgaatat aaagaaatat atgccagcag tattgatttc tgatttagat
     2221 agagttggat atcaacgctt tttaaataag tatgcgaaag accatgtaaa atccaccgtt
     2281 attaagttta ataaccatat tagagcatcg ttgaaagatg ccgtagaaga aggattaatt
     2341 ccgtttgatc caactagaaa agcagtaatc aaaggaaaag attcattgaa gccaaaagaa
     2401 gataaatatt tagattatga tcaatttaaa tctttaatga aactcgtaga agaaaacctt
     2461 tctgcacagt actcttctcc tatgctcgtg ttagttgctg gtgctactgg aatgcgattt
     2521 gctgaacttc taggattaac atgggaagat atcgatttcg aagatcaaat catcactatt
     2581 aataaaacat ggaattataa attaaatgaa tggggaaaaa caaaaaacga gacttcaaat
     2641 cggaaaat
//
LOCUS       DAZKAS010000223         2613 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000223 DAZKAS010000000
VERSION     DAZKAS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.229"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..306
                     /locus_tag="ACG8H7_002768"
     CDS             <1..306
                     /locus_tag="ACG8H7_002768"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8984814.1"
                     /translation="FMTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILD
                     EPFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            299..1327
                     /locus_tag="ACG8H7_002769"
     CDS             299..1327
                     /locus_tag="ACG8H7_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP8984815.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1383..1601
                     /locus_tag="ACG8H7_002770"
     CDS             1383..1601
                     /locus_tag="ACG8H7_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984816.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1672..2487
                     /locus_tag="ACG8H7_002771"
     CDS             1672..2487
                     /locus_tag="ACG8H7_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984817.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTESKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
BASE COUNT         1005 a          291 c          455 g          862 t
ORIGIN      
        1 tttatgacga aattaggtat tactttggac gacaatattt atgatgaaaa aatattatta
       61 tctggaggag aaaaacaaaa aataagttta gctagggcgc ttattaaatc agcttcagtt
      121 attatattag atgaaccatt tagaaattta gataatgaaa ctcgcgataa tttgagggat
      181 tatatactaa atacaaaaaa agaagtttgt tatataatta taactcatga tcacattcta
      241 gataattttt tggattcaaa tgatgaaata ttatatttaa ataaggagta tgataataat
      301 gaatagcaaa aaacttcaga tgggtgaaat tgttagaggt cctagtcaaa tatctattga
      361 tgttacatat aaatgtaatt ttagatgtaa acattgttac aatgcgagtg gtggagacaa
      421 ttgttttatg gataatgagt tgactgatga tgaactgtta gaacttacaa aagatatatg
      481 taatttaaag ccatataata tttgtttctg tggaggagaa actttactga gaaaaaaaat
      541 agttttatct tgtgccactt tattaaaaga ggcaggtatt cctaatattg ctattgttac
      601 taatggatca ttagtaactc aggatgttgc aaaagaatta ttcaaagcag gtattaatcg
      661 atttcaagta agtttagatg gaaaaaatgc tagtagccat gaagcactta gattatatcc
      721 aaacagtttt gaaaaggcaa ttaatgctat caaattattt agagaattgt ccgaagaagt
      781 ggatgttgct ttttgtccta ctagttttaa ttacaatcaa ttacgagaag tatatcaact
      841 agtaaatact cttaaagtga agtctttaag aattcaacca tttatgctta gtggtagggg
      901 aatgcaaaat agggaaatag tgccaactgt ttctcaatat agaaaactag tttctgatat
      961 taactatatt aaaagaaatg aagattatac caagatagat tggggagatc caattgatca
     1021 tatcatccga cacagaactt tattgaaaga tttttcgaat tttgtttcta ttaggtcaga
     1081 cggaggatta atggcatcac cttacttgcc aattcaagtt ggaaatatta aatgtcacag
     1141 tctttctaaa tattggcgag caggacttta taaagttttt tctttagatt tagttcaaga
     1201 tatagctact caagtttctt caatacaaga tatgagtaaa gaattaaaaa aaggacatat
     1261 taaatggctt aatgaagata tagaaattga tattctcgat gaatacaaac atgatatttt
     1321 aatttaggtt tatcattgga aagtttagat aaacaaagta cttttagaaa ggagggaaaa
     1381 aaatgatttc tgaacctcaa atgaataata tgtatgaaag cccaactata atgggagctg
     1441 gtgaagctgc gccaagagct tgttcagcaa ttatggtgtg tgtgttatta gcatctgcag
     1501 gagtaggtat ccaagcagga gcggtgtata cttatgctgt agttgctaca gtagctgtag
     1561 ctgcagccgc agtttggaaa acagtaggat tagttggtta ggaattaata ggagactaac
     1621 tgtctcctat tgattccaat gattaagcta aggaggaatt ttaagaaaat aatgaaatac
     1681 aataatttaa aaccgaaatt acttaatgag aacatagaat atataagaga agaaaattat
     1741 ggatatttaa ccatagttcc caaaaatgga actgaatcga aagataacgt tttcaatcca
     1801 acagtaagat atatattggc taactgtact ggaaaaaaca gtgtaaaaaa aatagcgaat
     1861 ggtttaaaaa agaagtttcc tagtttagaa tatgaaaaat tctatcaaga tgttgtagga
     1921 actattaaat ttttcaaaca ctataatatt attgatatag gagattatag tatggaagaa
     1981 aagaaaatat ataaaattga acaaaattta gaattagaat tactaaatga atcacattat
     2041 gaagaaattt ataattttat cacagatcat cctgaaagta tgtattttca tgaagatatt
     2101 tatcatcaag gaaatagtaa agaattagaa gttgtcctta gatataattt attttcgttt
     2161 acgagtgaat tttacttttt aagacataaa gagagaattg taggaatggt ttctataatt
     2221 gttaatccat ttcaaaactt atcctacagt atagtaaatt acctcatatg tgagcctgca
     2281 tatactacga ctattttgac aacagtatta gaaaatatta acgaagtttc aattttagaa
     2341 gtaaagaagc tagtgattca tttgctaaat tcaacagaag atgaatcact aatcccctat
     2401 ttaaaagatt cacatttttt taaagaagcg gtactatcta aagaatatag agagcatgat
     2461 gttttgagat acgcttacat ttattaaata aaatttctga tagtatttta ataattcgga
     2521 tcatatcata agatatagga gggaaaagaa gtgattttat tgattttgtc tctcgtatta
     2581 ctagttgtat ctaatctaat gaaaaataat cct
//
LOCUS       DAZKAS010000224         2595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000224 DAZKAS010000000
VERSION     DAZKAS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.230"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..594)
                     /locus_tag="ACG8H7_002772"
     CDS             complement(<1..594)
                     /locus_tag="ACG8H7_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP8984818.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIV"
     gene            687..1036
                     /locus_tag="ACG8H7_002773"
                     /pseudo
     CDS             687..1036
                     /locus_tag="ACG8H7_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1308..1598)
                     /locus_tag="ACG8H7_002774"
     CDS             complement(1308..1598)
                     /locus_tag="ACG8H7_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984819.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1806..2498)
                     /locus_tag="ACG8H7_002775"
     CDS             complement(1806..2498)
                     /locus_tag="ACG8H7_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP8984820.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          769 a          483 c          374 g          969 t
ORIGIN      
        1 gacaatcaaa tataaactaa ctatacttgt tagaacaaaa aaacaggcgt tgacttgttc
       61 ttgattagtt aaaaatatca ataaaggaat acttacccaa tatatttttc tatttatttc
      121 ttctcctctt aagagttgaa aaaacggata aaaagttagt aaactaaatg ctactggcca
      181 ctgatagttc aaggtggttg ctctccaacc ggtttccccc attactgtca atggtattaa
      241 acagtatata gagcaaacca ataataaatt tttaacattt attttctcaa aaacatactt
      301 gcaaagtaat actacaattc ctaacataat cgtagaattt aataatttcc atagcataaa
      361 gtgtttagaa aagaacatta agaatagttc tataataatt cttgaactcc atgtcttata
      421 atgttctatc gaagcaccca aatagccaag ttcatctaat ctacttgcat aaactacgtc
      481 atccgctctc atagtcggca tatacatatt aaagagtaaa aagatacccc aaatccctat
      541 tagtaacaag atcagttctt tgttattcgt tatttttcta ttatgtttgt tcatttttat
      601 actccttctt atatttccta atagagtata acatagcttt aagcattact taaagataac
      661 ttaaaagtta tcttttaaat acttaattgt taaatacctt atttgatgca aaagaaatct
      721 acgatagtca acaaaaaaat tttcattata tccagaaaaa tttttcttag ttaaggacct
      781 atggattgca gtaatgcaga attcgtcgaa taaactgcca aagacataca atcatattgc
      841 ttttaaaatt gacgaacaag atatagattc gtttgtctca aaaatccaaa tgttaggttt
      901 aactgtcgaa ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt acttttacga
      961 ctatgataat cacctcttcg agttacatac cggtactctc caggaacgtt taaaatccta
     1021 caattccact acctgagact agtaatgact agtctctttt tatggcctct gatcccccca
     1081 ttgagcgaat actaataggg cttcgaattg atctgttcaa tctttaagca atatccctca
     1141 aatgagtttg gaaagtttag tgattcatca caaatactta aatacatgta gttatatata
     1201 gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc tgcatccatc
     1261 tttccaaaac aagcggctag gacatttttg tctcagccgc ttgttttcta gtttattctt
     1321 tcatcttgtc ggaccttttt tcttgggtat agacttttct catattctac cacaattttg
     1381 ggtttcccta acacatgata ggatctcaca acaaattgct ttctattatt ataataacct
     1441 gtaacgacta acttcatccc ttcgtctact tcagctagga agttcaaact atgggaagca
     1501 atcaaacaat tgaccccatt taaactgaaa cggatcaacg gtctggcact aacttgcaaa
     1561 gtacggatct tatctactgt tccatatttt ttctccataa ccttcacctc aataccatta
     1621 tacgaacata cattcccttt gtaaagaaga aactctgttt tatctttttg actttttgtc
     1681 tattaccttt cttaaaatca tattggcact tcctgcgaag atgaaggaca tgtactaaaa
     1741 aaaagaggca gcagtataga ttactgttgc ttctttacta atttaggttt acttgttaag
     1801 aaagattatt tacgcaaatg ttcttcaaca atttgctgag atttatcagc tgtatcttcc
     1861 acttcatctt ttgctttttc taacttttga atatattgtt tcaagcttga gttttcagca
     1921 gttgcttcgt ctaatttctt ttgtgtatct gttaactgag tttctacatt tgttttttct
     1981 tgtgtcagtg ttgcgatttg ttgattcaaa ccatttacag tattttgtaa ttctgaaagt
     2041 tttgcttgtg cttcttgtag tcttgctgtc caatctgcat tcagatgatt gatttcatca
     2101 tttttttggt tgatctcatc tagtttgaca ttgatttcat tttgtttgct ttgtagttgt
     2161 gcatccttgc tgactaattg actttctaag ttttgttttt ctgctgccga ttgatttttt
     2221 tggttttcta attcatttac ttttgtttgg tattcttgga catctgcctt tgaatcttgc
     2281 aatgtctgtt ctttttgtgc tagtcgagcg gtaagatctg taagcgcttg ctctttatct
     2341 gtcaatgtat gatcaagctg tgtcagattc tcattgattg tatcaatatc tgtctctcca
     2401 ccccacaaaa taatttcatt tgcgtaccgc gtgcctgtgt agccaattag acatagaaat
     2461 gcaacgacaa tcccaccagt aataaatttc tttttcattt gcatagctcc ttaatccaat
     2521 acttttacat gctaaaagta agtgatctca aacacaatgt caagcgtttt taaattacat
     2581 gtgtaatttt aatat
//
LOCUS       DAZKAS010000225         2543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000225 DAZKAS010000000
VERSION     DAZKAS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.231"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1350
                     /locus_tag="ACG8H7_002776"
     CDS             <1..1350
                     /locus_tag="ACG8H7_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP8984821.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2543
                     /locus_tag="ACG8H7_002777"
     CDS             1484..>2543
                     /locus_tag="ACG8H7_002777"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFP8984822.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSES"
BASE COUNT          996 a          356 c          506 g          685 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt cta
//
LOCUS       DAZKAS010000226         2472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000226 DAZKAS010000000
VERSION     DAZKAS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.232"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG8H7_002778"
     CDS             <1..395
                     /locus_tag="ACG8H7_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFP8984823.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACG8H7_002779"
     CDS             398..1147
                     /locus_tag="ACG8H7_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984824.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACG8H7_002780"
     CDS             1159..1428
                     /locus_tag="ACG8H7_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984825.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACG8H7_002781"
     CDS             1433..1597
                     /locus_tag="ACG8H7_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984826.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACG8H7_002782"
     CDS             1590..1892
                     /locus_tag="ACG8H7_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984827.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACG8H7_002783"
     CDS             1889..2050
                     /locus_tag="ACG8H7_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP8984828.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACG8H7_002784"
     CDS             2047..2352
                     /locus_tag="ACG8H7_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984829.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..>2472
                     /locus_tag="ACG8H7_002785"
     CDS             2352..>2472
                     /locus_tag="ACG8H7_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFP8984830.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETG"
BASE COUNT          968 a          398 c          551 g          555 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg ag
//
LOCUS       DAZKAS010000227         2439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000227 DAZKAS010000000
VERSION     DAZKAS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.233"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..1055
                     /gene="pplA"
                     /locus_tag="ACG8H7_002786"
     CDS             120..1055
                     /gene="pplA"
                     /locus_tag="ACG8H7_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFP8984831.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1145..2248
                     /locus_tag="ACG8H7_002787"
     CDS             1145..2248
                     /locus_tag="ACG8H7_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFP8984832.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2261..>2439
                     /locus_tag="ACG8H7_002788"
     CDS             2261..>2439
                     /locus_tag="ACG8H7_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP8984833.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT          890 a          439 c          510 g          600 t
ORIGIN      
        1 ttgaaatatt acatgggtta tttatcaaaa atagttgact tataggctaa aaagattaga
       61 atgtataatg aatcacaaag aaaaattgtg gactagaaat ggaatggtgg ggtattaaaa
      121 tgaagaaaaa acgttttgta actggatttg ccgtattagc attttcagct ctagtgttag
      181 gcgcttgcgg cgcagatgac aacggaagct caaactcttc ttctgaatca tctacagcac
      241 aatcttcgac tgcaaaaagc acaacagaat catctgcaaa agttgtagct ggcggagatt
      301 tgcaagacgg cacatataaa ttagaagaaa aaaattattc aaatggttac cgtgcagtct
      361 ttgaaatggt tgtaaaagac ggcaaaatca ctgaatcaaa atatgataac gtcaatgaaa
      421 acggcgaatc aaaaacaaaa gatgcagaat acaacaaaaa catggaagca aaatctggca
      481 caaacccaga aaaatacatt cctgagttga atgaccaatt cttaaaagca caaagcgcaa
      541 gcggtgtgga agtcgtaact ggagctactc actcatctga aagcttccaa aactacgcac
      601 aacaattgat ccaagctgct caagctggta acacagatac aatcgaaatc gacaatggtg
      661 ctgatttgaa agacggcact tacaagcttg aagaaaagaa ctactctaac ggctaccgtg
      721 tgcaattcga aatgacagtt gctggcggca aagtaactga atcaaacttt gattatatcg
      781 acaaagacgg taaatcaaaa caagatgata cagaatataa tgaaaatatg aaagcaaaaa
      841 gcggtacaga accaaaaact tacatcccta ctctgaacga cgaattcgtc aaagcaatgg
      901 gcgaagaaga tggctcacct gctaatgtag aagttgttac aggtgctaca cacagctcac
      961 attcattcat catgtacgca caacaattag tgaatgcagc tgaaaaaggc gacactcaaa
     1021 caatcgaagt agataatatc gtaacagaaa aataatcaca taatcatact aataaaagtt
     1081 ggagggtggg acgatagcca gattaggcag tcgtctcacc tttcaaaatg aagagaggaa
     1141 aaagatgcgg aaaaaactac tattttcctt aatcacactg ggcatctctt ttatggtgct
     1201 aactggctgc agcaactcag caaataaaac aaccactgaa agttcagcag ctaccaagat
     1261 attgaaagat ccatattctg atgaacagtt cttgttaggg acttacgttc gtatccgtgt
     1321 gtatgatgaa ggcaaagaat cagcattgaa acctgccttc gatcgagtaa aagaacttgg
     1381 tgataaaatc acgatcaatc aaaaaggttc tgagatcgat gaagtgaatg agcaagctgg
     1441 gatcaaacca gtaaaagttt ctgatgacgt ctacacgtta gtcaagcggg cctacgaata
     1501 tagccaagat tctcaaggcg gattcgatat ggcgattggt gcaatcacac aattatggcg
     1561 aatcggtttt gatgacgcca gaaaaccttc ccaagaagaa atcgatcagg cattgaaatt
     1621 agtggattat cataagatcg aactaaatga caaagaaaaa actgtttatc taaaagaaaa
     1681 aggaatgatc atcgatttag gtgcaatcgc caaaggttac attacggacg aagtagtgaa
     1741 agtcttgaaa aaacaaggag tgaccactgc catcgttgac ttgggcggta atgtctatgt
     1801 cttaggccac agcccaagag gcgaaaacca agactggaca gtcggcatcc aagatccgaa
     1861 catggctcgc ggatctgttt taggttctat taaagaacgg aacaaaacat tagttacctc
     1921 tggaatttac gagcgttatt tagaagtaga tggcaaaaaa taccatcatt tgtttgatcc
     1981 aaaaacgggt tatccgttcg acaatgatat cgcaagtgtc acgatcataa cagataaatc
     2041 aattgatggt gacgggttgt ctacagcagt cttttcaatg ggtgtgaaaa aaggactgga
     2101 atacgtcgaa agcgaattga ataacgggac ggaagcgatc ttcgtgacaa aagacgataa
     2161 agtatatgtg accgatccaa tcaaagacac cttcaaatta tcggaggact cccattacac
     2221 gatgggtgac cgttctgaac taaaataagg agtacgaacc atgacactaa aaatcttttt
     2281 agaagtagtt gaaatacgaa caaaggtggc tagtgtattt ccttttgcgg taggggtatt
     2341 attctctatc gctttttttc atgagataca ttggctaaac acttttttat tttttattgg
     2401 aatgctagtt tttgatttag cgactaccgc tatcaataa
//
LOCUS       DAZKAS010000228         2433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000228 DAZKAS010000000
VERSION     DAZKAS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.234"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..2282
                     /locus_tag="ACG8H7_002789"
     CDS             129..2282
                     /locus_tag="ACG8H7_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFP8984834.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          832 a          426 c          539 g          636 t
ORIGIN      
        1 gtgaaacaaa aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt
       61 ttttcttttt tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag
      121 gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa
      181 taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc
      241 gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg
      301 gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga
      361 ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag
      421 ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag
      481 atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc
      541 tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca
      601 caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg
      661 tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca
      721 tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg
      781 ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga
      841 ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca
      901 gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag
      961 gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc
     1021 tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc
     1081 aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat
     1141 tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca
     1201 aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc
     1261 tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag
     1321 gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag
     1381 gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc
     1441 aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag
     1501 tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag
     1561 gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc
     1621 agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc
     1681 cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata
     1741 atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca
     1801 catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc gggaaacgag
     1861 tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag
     1921 tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg
     1981 cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca
     2041 aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg
     2101 cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag
     2161 acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca
     2221 taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat
     2281 aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat
     2341 agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg
     2401 tttactcggc aaaaaaggtt gtgacaaaaa ttg
//
LOCUS       DAZKAS010000229         2407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000229 DAZKAS010000000
VERSION     DAZKAS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.235"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACG8H7_002790"
     CDS             5..1150
                     /locus_tag="ACG8H7_002790"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP8984835.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACG8H7_002791"
     CDS             1321..1779
                     /locus_tag="ACG8H7_002791"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP8984836.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACG8H7_002792"
     CDS             1938..>2407
                     /locus_tag="ACG8H7_002792"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFP8984837.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAZKAS010000230         2398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000230 DAZKAS010000000
VERSION     DAZKAS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.236"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8H7_002793"
     CDS             207..2069
                     /locus_tag="ACG8H7_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP8984838.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG8H7_002794"
     CDS             2069..>2398
                     /locus_tag="ACG8H7_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8984839.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZKAS010000231         2369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000231 DAZKAS010000000
VERSION     DAZKAS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.237"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..2257
                     /locus_tag="ACG8H7_002795"
     CDS             71..2257
                     /locus_tag="ACG8H7_002795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP8984840.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT          911 a          398 c          470 g          590 t
ORIGIN      
        1 aacgaaactt taaataacca acttgaaata aacaaattca aagaagaaag atgggaagga
       61 aatcaatcag ttgaatgaaa aagaaattct ccgattacaa aaagaattga aacgctggga
      121 gaacaagtat aaaaagcttc aacaagtctt tcggctacaa gaagaaatca ttgctaccta
      181 tgaagcggct tatggcgaag cacccggact tattgaggaa gaagtacaag taaaagaaag
      241 tgaaggaaac agctctttct ttaaaatgat ggagaaacgt tcctataatc gcttcaatca
      301 agaacaaaaa gcagctatca catatgatat gaagaaacat cttcgaatca tagcaggagc
      361 tgggagcggc aaaacgcaaa caatctgtgc aaaagctgtt tatctgatga cgcaaaaaca
      421 agtagacgaa gaacgcatac taatgatcac ttttacaagg aacgctgcaa atgaactaaa
      481 aaaacgagta gataatttca gccagagaaa aaccgctgtc catatcggaa cattccatag
      541 tatctttttt aggatataca atgaaatttg tcgaaaattt cctgaagtag caatgcaagg
      601 aatacaagga gacttttcaa aggatactgc acaaaaagtg aatgctgttt tgcaacagct
      661 gatacgtaaa tataatcttt atatatttga tcaatatgga gaaaaaacga ttgcttctcg
      721 gttagactat tggcagaata tgaacctttc agtagaagaa atggttcagt tagttaaaga
      781 aaaatatgat tccattgata agaatgcacg acagccgata tcagaacgac tgtatggatt
      841 gctcacggaa ctccaagaac aaaaaagaag ccagcaattg ctagagttca atgatgtact
      901 ccaaaatctg aaaagcgctt tggagaatga ggagatccag cattatatcg gccaaaaata
      961 tgattatcta ttcattgatg aatttcaaga tactaatcca cttcaatggg aaatcgtgaa
     1021 acggatgaca aaggaaaaca ggatcaagtt gattgttgtt ggagatgatg atcagagtat
     1081 ctatggattc agaggatcag agccagcata catcaagaac tttgaaaaag aatatcctac
     1141 caaaactctt ttcttattga ctaattatcg ttctcgggca gctattgtcc aaggagcaaa
     1201 tagattgatc agctataata aaaatgatcg gataccaaag gcgatgattc cagcacaaaa
     1261 agaagcaggt gttatggaag cctatttgtt ttctgatact aaagcagaaa gtcagtggct
     1321 aatcaaacaa attcagacat ttattcaaaa aaatgggaaa tataaggaat caatcatatt
     1381 ataccgatca gcttcacaaa cgcagcagct tgtccaatct ctgctgaaaa cgaatattcc
     1441 ttttgtactg gaagcagata gtccatatga aggtatcttt ggtatcaagt ctttccaaca
     1501 tttctatcaa aaaattgttc attggcaaac agcactgaat ctgcaaaaga aaaatcaggc
     1561 ctaccagcaa ttgctccggc agctgatggc agactgctac ctgaaaaaaa gtgaatgcga
     1621 tcaatacttt tctcctaaat atcaaaatat agccgttgca gattatattt tgtcgataag
     1681 ccccaattta aaatcgaaat ctgcagaaat ccgagagttt gaggaagcgt tgcgtaaaac
     1741 agaccagcat caagaaatct attcgcttgt tcaaagctat cttcatctgc cacgtatagc
     1801 taaggaaatc gaccagagtg atcaagaatg gatacgagaa gaagtaaagc agcatgctac
     1861 tttcagaaga ctccaagcct tatcgcaaga aacccaagcg acagctaaag aattaaaaac
     1921 ccgcttgatt gcttacagac aaggaaaact tgatgcacta tgtatccaga gcattcacaa
     1981 aagtaaaggt cttagttacc gaaatgtctt tttgattggt tgtaacgaag gaagtttgcc
     2041 ttataacgga gcgacagaaa agaaagtaat cgactttaat gaaataaaag cagaaccagt
     2101 gacgacgata gaagaagaac gaagactatt ttatgttgcg atgactagag caagaaatcg
     2161 tctgtatcta tgtgtacctc aaatgaaaaa ttcaagaaaa ttgaaagtat cgagatttat
     2221 caaagaaacc gggagtcgtg tgaaaaaagg aaagtgatag aagagagggt gtgacagaag
     2281 tggtcagttt caagaaagaa gaaggaatgt acgaaaattg cttctcaaat ttttgtgaaa
     2341 tttcgactta tttttcgaag aagctactt
//
LOCUS       DAZKAS010000232         2362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000232 DAZKAS010000000
VERSION     DAZKAS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.238"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(158..2242)
                     /locus_tag="ACG8H7_002796"
     CDS             complement(158..2242)
                     /locus_tag="ACG8H7_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFP8984841.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          687 a          524 c          362 g          789 t
ORIGIN      
        1 atcaaaaaaa taagttattt tcttttattt cattgacctt attgaatttc aagctatttt
       61 gaattttctg caaaattaaa aacattagtt attttataat cctatctatt ttctttacat
      121 ttttcattaa gaaaaggata taaaaaaaag aggacgttca ttcgtcctct ttttttacat
      181 gcaagcttcg catggcattc aaaacagcta atactgtcac cccaacatct gcaaatactg
      241 catctcccat tgatgcaaaa ccaaaagcac ctaaggcaag taccaaaatt tttatcccaa
      301 tagcaaagac aatgttttgt ttgacgattt tcaatgtttt ctttgctaaa tggattgctt
      361 ctgcaatctt cgatggttga tcattcatga tgactacatc tgctgcttca attgcagcat
      421 cgcttcccaa tccacccatt gcgattccta catctgctct tgctagcacc ggtgcatcgt
      481 tgattccatc tccgacaaat gcggtctttt tattgtttct ttgtaagatt tcctctaacc
      541 gctgcacttt atcttcagga agcaattcgc tatatatctt gtccacgcct acttgcttcc
      601 cgatatgatc agcaattttt ttcgagtcgc ccgtcaacat aactgtgttc ttcacaccag
      661 cctgttttaa ttgaaccaat gcgtctacgg catcttcttt caaggtatct gcaataacaa
      721 gataaccact gtaactttga tcgatcgcta gataaagcaa tgtaccgatt tcttgactta
      781 aaggaaaaga gatttcaaat ctttccatca atttgtgatt acctgctaat acttgtttgc
      841 catcaacttc agctaagatc ccttcacctg caaattcctg gatattagtt gttggtaaaa
      901 gcggacctgc gtagccttct aaaacagatt gagcaatcgg atgtgtagaa aactgttctg
      961 cactcgcaat atattgcatg aatttctttg gatcgatttt tgtatcgatc gtctggatcg
     1021 aaaagtcacc tttcgttaat gtgccggttt tatcgaatac cactgtctcg gtttgcgcaa
     1081 gcaactccaa gtaatttgaa ccttttacta atacaccgat tttacttgcg cctccgatac
     1141 cgccaaagaa actaagtggc acggaaatca ctaatgcaca tggacatgag atcactaaaa
     1201 atgtcaatgc tcgatagatc cattcagaaa acgccgctcc agaaacaaca agtggaggta
     1261 aaacagctaa taaaacggcc aatcctacaa cgactggtgt gtaatagcgc gcgaatttag
     1321 taatgaaatt ttcagctggt gcttttttac tgctggcatt ttctactaat tctaataatt
     1381 tgctgatagt agaattttca taagattttt ctacttgaac taaaaatggt ttatttttat
     1441 tgataaatcc gcttaatact gtttcaccaa catatacaga tttagggact gattctcctg
     1501 taagtgctga cgtatcgact aatgattctc cttcgatgat cgttccatct aacggtacac
     1561 gttcaccagg cttcacaagt acaaactgac cgatctgcac gtcttcaggg ttcacggtca
     1621 tcaatccttc cgtcgtctgc acgtgagctg tttctggacg gatcgccatc aattcgcgaa
     1681 tcgattttct tgaatgactg actgccaatc cttggaaata ctcgccgatt tgataaaata
     1741 gcatcactgc tacggcttct ggatattcac cgatcaacat agcgccaatc gtagcaacag
     1801 acataagaaa attctcatca aagatttttc ctttgacgat attcatcact gccttcttca
     1861 ctacatcata tcctatcagc agataaatca ataaataccc tatcaagcga attggttcat
     1921 taaaatcaaa taatagcaat aaagcgatac ctgcaaggga aaataaaatc cggaacagcg
     1981 tatttctctc tttttcattt ccatgttcag atactttttc cttatctatt tctgttactt
     2041 tgacatctgg ttctaattcc ttcactactt tttttgtttc ttgttcaatc gtagccaaat
     2101 gatcttcctc tgcgtcaatc gttagttttc cagaagtgaa gtttaccgtt gcttccttga
     2161 ctccattgat cttctgaact cctttttcga ttttcattgc acagttcgca caatctaaac
     2221 cttctaatct gtaacttttc attttaatga cctcctcgcc tcaacatatg aacatctctt
     2281 cataactaat tataaatgaa tggttattca tatgtcaagg tatccgaaag aaaaaatgaa
     2341 aaacatctgc atcggaaaat tt
//
LOCUS       DAZKAS010000233         2361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000233 DAZKAS010000000
VERSION     DAZKAS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.239"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8H7_002797"
     CDS             <1..1076
                     /locus_tag="ACG8H7_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP8984842.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
     gene            complement(1236..2141)
                     /gene="xerC"
                     /locus_tag="ACG8H7_002798"
     CDS             complement(1236..2141)
                     /gene="xerC"
                     /locus_tag="ACG8H7_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP8984843.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          765 a          471 c          406 g          719 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttgactta
     1201 tcaccattag actaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga
     1261 tagttttttt gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg
     1321 gcatgtccta gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta
     1381 gcaaatgtat ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc
     1441 atttgattca agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca
     1501 aaaacgatat catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa
     1561 tattcttgca gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct
     1621 ttaccatgga tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta
     1681 cactcactta aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa
     1741 tccaaaggac tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa
     1801 aaacgtggca gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt
     1861 atcacttctt ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg
     1921 atagaatttc tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc
     1981 gtaaaatatt ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa
     2041 tggagaatgt cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc
     2101 aaataacgca aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt
     2161 cccagagaat aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc
     2221 aatatttcta agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt
     2281 aagaaaaacg caagaaaaag ggctctataa tttatgggac tgatgaatca gaattgattc
     2341 ttcagcccca ttttcatttt a
//
LOCUS       DAZKAS010000234         2346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000234 DAZKAS010000000
VERSION     DAZKAS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.241"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..326)
                     /locus_tag="ACG8H7_002799"
     CDS             complement(<1..326)
                     /locus_tag="ACG8H7_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984844.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIAT"
     gene            629..814
                     /locus_tag="ACG8H7_002800"
     CDS             629..814
                     /locus_tag="ACG8H7_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984845.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            826..1074
                     /locus_tag="ACG8H7_002801"
     CDS             826..1074
                     /locus_tag="ACG8H7_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984846.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            1105..1284
                     /locus_tag="ACG8H7_002802"
     CDS             1105..1284
                     /locus_tag="ACG8H7_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984847.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1327..1797)
                     /locus_tag="ACG8H7_002803"
     CDS             complement(1327..1797)
                     /locus_tag="ACG8H7_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984848.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1884..>2346
                     /locus_tag="ACG8H7_002804"
     CDS             1884..>2346
                     /locus_tag="ACG8H7_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP8984849.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVM"
BASE COUNT          831 a          345 c          422 g          748 t
ORIGIN      
        1 gtcgctatca ggatatcttt tacgcgctgt atttgttcgg gtgtcaatgt ttccccacca
       61 taagacatat taacatttga gtctagtagt ttatcaagtt caaccacttc ctcttttgta
      121 gcccatttgg gaacattatt atttcccaat aaataatcag ttgtgacacc aaaataatca
      181 gcaactttct ttaagttctc agattttggc gaggctttat cccatcttct tatttgtcca
      241 ttagagatgc ccacctgtct ttctacttct gctatagtca catgcttttc gtctgctaat
      301 tctttaatct tagtaactaa actcattatt atcaaccttt caaagctgaa aagaaaataa
      361 atagcttaaa agttatattt ttggttgaca attagctttt aagctagtat atttaattcg
      421 taagctaaat tgttagctaa ataagagcaa caaaaaactc tactaattta aaacattctc
      481 tcggtcgcca aacttagaaa tgttatttta gaggcttttt ataagtctta tttaactatg
      541 tattcataat agcttaaaag ctaataggtg tcaacgattt agctaatttt ttagcttaca
      601 aattattttt tagaaaggag ctatttttat gtctgagaat ttagacttaa aaattcgagc
      661 ggagatgaga aaaagaagaa tgactttcaa agaactagct gcgcttatcg gtatttcagg
      721 agcttattta tcagatattc taaacggcaa tcgtgatgga aagaaagcac aacagcatat
      781 cgaaacagtg aaaaaaatat tggacatccg ataggggggt aggcgatgac aaaactaaaa
      841 aaacaagatt ttgtaaaaaa atacaattat tctccatcta cttatcaacg tcgaatgtcg
      901 gaactaaaaa atacagcaat tttctcagcg gcgtatgaac gggtcacagg acaagaagtt
      961 tggatcaata cagaattata cgataaattt ttgtctttca aatcctataa caggttacgc
     1021 acaagaaagg taacacctaa agaatttatc gagaagcatt tagttgattt ataaaaaaca
     1081 gaacattttg agaggtgaag gttaatgggt aaattcaaca gagcattggt attcagcgca
     1141 ccgctaatca tctacgcttt aggactttgg ggaagcaggc aagcgttgat aggaacgatt
     1201 gtttacatgg tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa
     1261 aagccaaccg agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac
     1321 tattactcag tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc
     1381 aacaattttg tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag
     1441 gagtttatta aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt
     1501 aggaggaata tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc
     1561 tttagcaatt aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga
     1621 aagttctaaa ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt
     1681 ataaatcata tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat
     1741 tccttttatt tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca
     1801 ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta
     1861 taccaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg
     1921 tgactacgag cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag
     1981 atattgatgc actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg
     2041 aaccagattc atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct
     2101 tgctggctat ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag
     2161 cattcaacga aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata
     2221 tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag
     2281 atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag
     2341 ttatgg
//
LOCUS       DAZKAS010000235         2313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000235 DAZKAS010000000
VERSION     DAZKAS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.242"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..445
                     /locus_tag="ACG8H7_002805"
     CDS             191..445
                     /locus_tag="ACG8H7_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP8984850.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ACG8H7_002806"
     CDS             446..763
                     /locus_tag="ACG8H7_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP8984851.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="ACG8H7_002807"
     CDS             1322..2257
                     /locus_tag="ACG8H7_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984852.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILITRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          847 a          312 c          433 g          721 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga aacgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttta ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataactc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag gaaaatgtct
     2281 aaacatgtta gtaaatcaac aaaaaaacgc ata
//
LOCUS       DAZKAS010000236         2273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000236 DAZKAS010000000
VERSION     DAZKAS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.243"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..985)
                     /locus_tag="ACG8H7_002808"
     CDS             complement(<1..985)
                     /locus_tag="ACG8H7_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFP8984853.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACG8H7_002809"
     CDS             1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACG8H7_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP8984854.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..>2273
                     /locus_tag="ACG8H7_002810"
     CDS             1810..>2273
                     /locus_tag="ACG8H7_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HFP8984855.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQR"
BASE COUNT          705 a          438 c          454 g          676 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaa
//
LOCUS       DAZKAS010000237         2262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000237 DAZKAS010000000
VERSION     DAZKAS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.244"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..>2262
                     /gene="acm"
                     /locus_tag="ACG8H7_002811"
     CDS             104..>2262
                     /gene="acm"
                     /locus_tag="ACG8H7_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFP8984856.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYK"
BASE COUNT          890 a          348 c          447 g          577 t
ORIGIN      
        1 tagttaaaag attggaaatg agtgggggag gaattgaaga aaggattttt gagagatgat
       61 atagtaggtt ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa
      121 tattatttta tcaatgttat ttattattac taatataaca tcactgattc ctgttcatgt
      181 ctatgcggat gcaggcagag atatcagcag taatgtcact tcgttgaccg tggacccaac
      241 gaatattaca gatggaggaa acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa
      301 tatccaacca ggtgattatc tttggatcaa ttggccaagt gaaggaaata ttcgcggtga
      361 aggattccaa aaagaaatcc cattaatgat tgaaaataaa aatgttggaa ctttaacggt
      421 cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga
      481 aggttggggg caatttgaga tacaggccag aaacgtaacc gataccggtg aagaaaatac
      541 gggaccattc accgtgacga gcggtgataa aacagctact gtaaatgtga caaagccagc
      601 ttctggttct tcttctagcg ttttttatta taaaacagga gacatgctac ctgaagatac
      661 taagcacatt cgatggtttt tgaatattaa caataacggc acttatgttg aacaaccagt
      721 taaaatcagt gatgaaatcc aaagtggaca aagattagat ccaagtacgt ttgaaattaa
      781 tcaaatacac ttaggtgaac agaaggttta tagaggagaa gagggaatcc aacaattttt
      841 acaagatttc cccagcgcta catttaattt ttcagtaact gataattaca ttgaaataac
      901 tataccaaaa aactttgtca atcttaggaa aataatggtg agttataaga cgatcataga
      961 aaacccagaa caaataaatt ttgagaatca ttcggaagct tggtttaaag aatttaataa
     1021 acctgctgtt gatggagaaa gtttcaatca tacagttaaa aatatttcag cttctggtgg
     1081 agtcaacggt acagttagag gcgaattgaa gattttcaaa tatatcaatg acacagagat
     1141 tggtatccca aatgtgactt tcgaattaag acgagcagat gaacagccta ttcaaggaca
     1201 gtctagtatc ctgttgactt cgaatgaaca gggagaagct agtatcaaag gcctgcaagt
     1261 aggagactat gttgtaaaag aaaaagaagc acccaattgg attgattttg atccactaag
     1321 ttcaaatgaa ctcaaattct ccataaacga aaatgataca gagggagtca gtttacctat
     1381 ttataacaag aaaaaggtta cgaacatcac agcaactaaa atttggaacg gtggaaccac
     1441 tccaagaccg agtatttatt ttaaattatt cagagcaagt acaaacaatt gggaaccagt
     1501 tcctgatgca gaaacaaaac gattagacaa tggaataact tcagtgactt gggaagatat
     1561 ccaacagtat gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa
     1621 cggaaatgat tatgtccctt caggttatca aaaaattgaa aacgggctag tcgttacaaa
     1681 tgagaataaa gaagtcattt cagtctcagg acaaaaaaca tgggaagaca atgagaacca
     1741 agacggcaaa cgaccgacga cgatcacagt aaatctatta gccgacggtc aaccgatcca
     1801 acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc tgccaaaata
     1861 cagagacggg caagaaatca tctatactgt aacggaagac aacgtgcccg aatacagtac
     1921 gacgattgac gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa atatatctat
     1981 accaggtagc catataacac cgtggaaaaa ttttccagag aagacagaga gagagaaaat
     2041 tagaaatcta ttctctcaaa aattgcaaat atttgctcat acaggagaat ccagcgaaca
     2101 aagaagcaat tatattagta agagagctgt acctaaacag ataacaaata aatcaaaaag
     2161 cattcttcct aaaacaagta gtaaaaaatc tagttttgct gtaatcattg gtttattgat
     2221 agtaactatc tgtgctggga tattcttaca aaaatataaa aa
//
LOCUS       DAZKAS010000238         2233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000238 DAZKAS010000000
VERSION     DAZKAS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.245"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..544
                     /locus_tag="ACG8H7_002812"
     CDS             <1..544
                     /locus_tag="ACG8H7_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8984857.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="ACG8H7_002813"
     CDS             complement(900..1472)
                     /locus_tag="ACG8H7_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8984858.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /gene="fic"
                     /locus_tag="ACG8H7_002814"
     CDS             complement(1488..2093)
                     /gene="fic"
                     /locus_tag="ACG8H7_002814"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFP8984859.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DAZKAS010000239         2221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000239 DAZKAS010000000
VERSION     DAZKAS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.246"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(206..1105)
                     /locus_tag="ACG8H7_002815"
     CDS             complement(206..1105)
                     /locus_tag="ACG8H7_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP8984860.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1115..1471)
                     /locus_tag="ACG8H7_002816"
     CDS             complement(1115..1471)
                     /locus_tag="ACG8H7_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP8984861.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1826..2122
                     /locus_tag="ACG8H7_002817"
     CDS             1826..2122
                     /locus_tag="ACG8H7_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFP8984862.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          692 a          422 c          380 g          727 t
ORIGIN      
        1 ttatattttt ttttgtgatt atcgttcaaa aaaagaatac acgcttgcat ctagtactct
       61 gcgagttaca aatcatccga tcaaataaac attcgctgat tattttccgc aaaaaaagca
      121 gcatatccac gataaatgga tctactgctc ttacttatcc ttatgtttta caaggtattt
      181 tcttttacac attactaatg ctgtctcaat acggaacgat tgtcatcgct tgttcaaatt
      241 cctcttgcaa atgttgtgtt tgataggcct gaaaagctgg gtgatgggca cgcaagagga
      301 cacgtaattt cataggatca tagctgtcta gcaactgttc tttcaagaca aaatctcggg
      361 tcagctgcca ttcataataa taaccaaaag gtctgcagct gtaaatcgtg tgaccgatgg
      421 atttatcttc aaagcgacga tgcgtatggc caaaaactac ttgtttgata tttgaataac
      481 ggtcaagtaa ttctccgaat gattctgatc ctaaaaatgc atttaatttg ttccaacgct
      541 cataaggagc agattgatac atgataaatg cccgtttagg aacgaaatga gtggataaaa
      601 tcacctcaag tttttttgct tccagctggt ctagcaaatt ttttgcagct gcaagcattt
      661 ctgcattgac ctcgagatct gttttcccac gctggatgaa ccgatcatac caatacaaac
      721 gcttcattcg tacgattgcc tcgtaatctt tcaattcgga aaactgatag tcgtaccagc
      781 cattcatccc taaaagaacg gtctgctcat ttaaagcaat ataccgttca tttaaaaaat
      841 gtgggtctgg aaaatggtcc atcatctgtt cattgggcac atctgccaac tcgtgattgc
      901 cgaaatgaaa tgtcgtagat aatcctttat tgacgaaaaa ttcgacaaca gctaggcaac
      961 ggtctacttt attagccgta tctccggcta gatgaagccg agtgatcttg tgcttttgca
     1021 aaacttgcca aagctgcatc agctctgatt cgccgaattt attgatgtcc acatgtaggt
     1081 cgctgatgat cgccaatttt cccattttat ccctctagtc ttttcgaaca acttgcaagt
     1141 tgccttttct caagccgctt ggtcctttta attgtaaact attataagtt ggttctaaag
     1201 caagtgtgga tggatcatcc atgtaaagta ttgcactgtc tttgattttg attggtccaa
     1261 ctggtgtatc agccactaca gaataaagac ttagatctgt ttgtgtatct tttttgacaa
     1321 tgatcaatcg aaatgccgta tccaattggc aactgacttg gctttctgca aatggaccgt
     1381 caccgttttc gatatctaat aaaagttgat cgtctgcttc taatttattc ttcaatactt
     1441 cttgtgcttc ttttgtcact tctaatttca tctgtatcac ctcatgtcat agaataatca
     1501 ctttattcat cagatgcaac taatttgtct ggttctgact gttctcaatt tattggaaag
     1561 taagtcattc gtttattatg atttctctta tcacaaaaga gacaactttc ccttttcttc
     1621 gttcagtttc ttgaacggaa aaagacccaa aaagcaacct gttatttgtt taaccttatt
     1681 cctttcttaa ataacaaaaa atactataaa aaataaaaaa cctttaacaa ctattggaaa
     1741 aagaattact ttttgttcac ggattattaa caactcttcg gtagagtaaa cttatcaaat
     1801 aaaagacttc tttggaggaa tcactatgaa ggaatatata aaaaacatct attttatcga
     1861 agaaacacaa aatatcgagg gttcttatat tgaagtaaaa acactctttg tcaatgaaga
     1921 taaaaccaaa gcattggata tttataaaaa attagcttca aaaaaaacga atagttttgg
     1981 attgatatta agcgagtata aaatcaaagc agaagagagt tatttctatc aattattaaa
     2041 acggtggagt aagcttcccg ccgattttta tcgaaaaatg cagatcatca actatcaacc
     2101 gcttgctgag acacatgcct aaatagaggt tgtgaaatca gtgctttgag ctaataccga
     2161 aggtcagctg attgaacatt gtttagaaga gattgtgaca actgcgattt gctttgagta
     2221 a
//
LOCUS       DAZKAS010000240         2157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000240 DAZKAS010000000
VERSION     DAZKAS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.247"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8H7_002818"
     CDS             complement(<1..914)
                     /locus_tag="ACG8H7_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFP8984863.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8H7_002819"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8H7_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP8984864.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          731 a          381 c          417 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaa
//
LOCUS       DAZKAS010000241         2081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000241 DAZKAS010000000
VERSION     DAZKAS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.248"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..453
                     /locus_tag="ACG8H7_002820"
     CDS             61..453
                     /locus_tag="ACG8H7_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984865.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(626..829)
                     /locus_tag="ACG8H7_002821"
                     /pseudo
     CDS             complement(626..829)
                     /locus_tag="ACG8H7_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            795..992
                     /locus_tag="ACG8H7_002822"
     CDS             795..992
                     /locus_tag="ACG8H7_002822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984866.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1035..1610)
                     /locus_tag="ACG8H7_002823"
     CDS             complement(1035..1610)
                     /locus_tag="ACG8H7_002823"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP8984867.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1765..2081)
                     /locus_tag="ACG8H7_002824"
                     /pseudo
     CDS             complement(1765..2081)
                     /locus_tag="ACG8H7_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          673 a          355 c          313 g          740 t
ORIGIN      
        1 acgtaaatca attatttttc attttacttt aactaataat gtttatttgg agggattatt
       61 atgttagatg atagattgac gtgttctaat tgtggtacat tattttacta tgaggaagtt
      121 agaaatattg taaaacatag aaaaaaagaa atgcctatac gttgtccaaa ttgcaatgaa
      181 gttattaagc gaaagttgtc tcatggttat tttgtcagct acgaactata tgaagaaagt
      241 aaaataaatc tgaaaaaaaa accaaaatac tataaaaatt tgaaaactgg agctattata
      301 actgaaaaag aatttaaaaa tttagtgact gaagaagaaa gcaggtttta taatttgtct
      361 ggtaatattt gccctggatt tatggatcag attattgaaa atgagtcaat tgaatcaatt
      421 gaaaactatg ttcctgttga cttaccaaaa tagaaaaaat aatcctgttt ttacagtgaa
      481 ttattttaat tttgattttc aataaaaata tattacaata ataatcgtaa tagctaatga
      541 aacaatttct caatcttttt accactccaa acagggagcg tcaataattt tgtgtaaata
      601 actcgtcctt ctgcaaaata atgggttact cagtaaacat tgaagctaat gtatccgtca
      661 cttgttggaa gcctttatgg cttctattta agaatttttg attgtatgta tcaaagatgg
      721 atactaggaa acgctctagt gattcttcat tttgaaactg ctcttttctg cggctgtatt
      781 tcttgatttg tttattgaaa gattcgattg tagaaccaga aggaatggag aacacttttg
      841 aactttacaa caaaactact gttgataaat taattccaat tttagttcca actgatttaa
      901 atggtcatga tgttttactt cgttttaacg gatttttagg tgaggattat agatacctaa
      961 aaatttctga tcctcaacct ttaacagaat aataaaaaac gtgtaatcaa ttatttgatt
     1021 acacgttttt tatttcaatc ttcgttgtag tagtggttgc gacataccga aaggtaatct
     1081 tcattacctc ctactacaat ctgttcacct gaataaactg gaataccatc aacaatacga
     1141 gcattcatta cagccttttt attgcaaaaa tggcaaatag ttttaatttc ctcaatttta
     1201 tcagcaaatg tcaataatgc ttcggatccc ttgaagagat tattttgaaa atcattcttc
     1261 aatccatagc aaattactgg tacttcctca ttcattacga taagtgatag atcaataata
     1321 ttctctctac ttaaaaattg agactcatcg accaaaatac aatcaacttt tccgtactga
     1381 tcaataatct ccactaattc tccgtacaca tccatttcag aagtaattgc aatagctggt
     1441 ttcgaaacac ctatacgact ctcaactttt ccaaatcctg atcgagtatc tattgcgctt
     1501 gtcattatga taactctctt cccttgttct tcataattat gagccacttt cagtagctca
     1561 atactctttc cactattcat tgttccatat ctgtaaaata attgtgccat taaatttatt
     1621 cccttctttt acaattcaat tattccttaa ttccatctgc ttttatgcag ctgctcgttt
     1681 attccttgat atgtctataa catctactat ccctaaaacc aatttataag ccattagaga
     1741 tggtattcct gaacatctct catattgttt tttgagtggt atatcatctg aaaaactgaa
     1801 ggaatcatga aaatagcgac atacttatca tttagagttc taactttaaa tgcatttact
     1861 tcttcagtca gcactttagt catgttggac atggtttggg gcgtgtagtg atgcccatac
     1921 attttctcaa tcaagtctgc gatttccgac atggtaatac cttttcggaa gaggtgaatg
     1981 acggtctcct ctaatgtgtc attcgtccgt ctataagcag gaacagtctg ttgcttgaac
     2041 tcgccattgc ggtcgcgcgg aatctggaga tgaagttccc c
//
LOCUS       DAZKAS010000242         2072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000242 DAZKAS010000000
VERSION     DAZKAS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.249"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            687..1019
                     /gene="traE"
                     /locus_tag="ACG8H7_002825"
     CDS             687..1019
                     /gene="traE"
                     /locus_tag="ACG8H7_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP8984868.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1638..1979
                     /locus_tag="ACG8H7_002826"
     CDS             1638..1979
                     /locus_tag="ACG8H7_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP8984869.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          725 a          279 c          433 g          635 t
ORIGIN      
        1 aatcacaaac gtagcctttt acgtcataag tgttaaaaga aataattaat ttcaaatata
       61 gaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata cgcgaagaga
      121 gataatttca ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg
      181 aatattattc gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt
      241 gattttcgtt ctgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa
      301 aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat
      361 ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata
      421 acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata
      481 ttacgatgaa tctatgccta agcacaatgt tataaaaatg tccgtgtggc tctttgccgt
      541 atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct tttttgtttg
      601 tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt gttaaaaata
      661 tttaaagaaa ttgggtggtt tattttatgt catatacagg aattttgagt ctcaaagata
      721 tttgtcacta cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg
      781 gccaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac aaagtctcag
      841 aggaaatgat ttattacatt gggaaacaaa agcaaataat tttaaaagat cctatcccct
      901 taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta ctgattggtc
      961 gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgtttg atcggctgaa
     1021 ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat attgtcgaga
     1081 cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct atcaacaatt
     1141 cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg
     1201 agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata agcgcagagt
     1261 aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta atcatgagca
     1321 gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta
     1381 atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat acttgcgagg
     1441 acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc tcgtttcgga
     1501 cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac gataaaatat
     1561 gaacaagtcc tgaataaagt gaaaaaattg atataaaaca tagagaaaat gaaatttacg
     1621 taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc acacatacta
     1681 ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga agaggcggaa
     1741 attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat caaacattga
     1801 ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat gaatggttta
     1861 aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt
     1921 tgagtgatta tattgtcagc tttgaaatca ttgaatttgt agcggataaa gaagaataaa
     1981 aactggagga ataaatttta cagataaagg cttagaagga gtcacgatga ttaataaaaa
     2041 ggtagtatta ccttaggcat tgagtaagga at
//
LOCUS       DAZKAS010000243         2040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000243 DAZKAS010000000
VERSION     DAZKAS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.250"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACG8H7_002827"
     CDS             <1..1872
                     /locus_tag="ACG8H7_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFP8984870.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAZKAS010000244         2027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000244 DAZKAS010000000
VERSION     DAZKAS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.251"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..168
                     /gene="mobC"
                     /locus_tag="ACG8H7_002828"
     CDS             <1..168
                     /gene="mobC"
                     /locus_tag="ACG8H7_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFP8984871.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVAPDDAQRLSENLKIMQ
                     KELQKIWQQLK"
     gene            150..1064
                     /locus_tag="ACG8H7_002829"
     CDS             150..1064
                     /locus_tag="ACG8H7_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP8984872.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1309..1998
                     /locus_tag="ACG8H7_002830"
     CDS             1309..1998
                     /locus_tag="ACG8H7_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984873.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
BASE COUNT          807 a          383 c          372 g          465 t
ORIGIN      
        1 caggcaatcg cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgccaagtgg
       61 tgcaatacac accaacacga cgttgctcct gatgatgctc aacgtttatc cgaaaaccta
      121 aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagt cgaaccactt
      181 catgtagccg agcaatcaat tacgcagaac cacgagcaac cgtcaaaaca ggaataaact
      241 gtgacattga ttatgccaaa agtgaaatga aacaaatacg cacgctttat ggaaaagacg
      301 atcgtgtaca agcacactta ttgattcagt cctttcgacc aggagaaatt accgctgaaa
      361 aagccaatca actaggggca gagtatgcga aaaaaatagc ccctaaccac caaatcgcga
      421 tttacacaca tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc
      481 ttgaaactgg gaaaaaattt caagcccatg ggcaagcatt tttaaacaaa tgttatgacc
      541 taaatgacga gatttgtctt gaacatgact taacaatcac ggaacgagga aagaaaaccg
      601 aaaaacgaac catgtctgaa attaaattaa aagaaaaaaa tgaaccggtt tggaaagacg
      661 aaattcggtt tgcgattgat caaacaatga aaaacaaaaa aactcgaacg tatacccaat
      721 tttgtgattc acttaaaaca ttcggcattc attgcttgaa tcgcggaaaa aattttactt
      781 acgaattggt ccacaaaaag aaaaaagtac gatccaacaa actaggaaag gattacgaaa
      841 aggagacgat tttacatgag ctggacagac gagaacgaac ctacaatcaa caacgaacca
      901 acgagctacg aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac
      961 aacgaacttt tacgcctgaa caatcggaaa ttagaccgac tcgaagaaac catgaacaac
     1021 acgttgaacg aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacatc
     1081 gaaaacgaaa taaagagaca acgtgccttt cttgcgcctt atacccaaga aaggatacaa
     1141 actacgatgg attcacaagt caaaaaactc caccaagcgc tattagacca gcgaaaccaa
     1201 gaacaaaaaa aggctttaat tcaacgatcg atggatattc ttgccccctt atgttgctta
     1261 gtactaacat tattagcctt ttggttggta tggggagaat aaacagacat ggcattctat
     1321 ttaccctatc tactcatttc tgccagtatt ttaggaatta tttggcttag ctataaggct
     1381 tatcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagcgatt tttattggtc
     1441 ggggttatat ttgccttatc aatcgtttta agtggtttat tcggcgtttt agagggtaat
     1501 aaacgtgttt caagaagcat tttattaggg gatatcactc aaaagtatga agcagcatgt
     1561 gataaaaaga aaaaagagca agcattagca caaaaaatgg aggaatttac cgcttgttat
     1621 gaggacatga acaatatttt tgtggatcaa gagaaacgtt taacagataa aaacatgaaa
     1681 aaactcactc gtttttatca aaacttacca gaaaaacagc aaaaagaggt acaagaaaaa
     1741 tatgagcaag taaaaaaaga cgtgcaatat tttaaagata cgcagacaga agaagcttgt
     1801 tgtaacttat ttagcgatac aatccctttt tcaaccagtg aacaagaacg aaaagaacgg
     1861 caacaatcgg tcacatacga acgttatgaa acactgcttc agcaagcaac aaacatacaa
     1921 aatccaacga aaaaagaaac ggcattaaat tatctagaaa gtgtcaagga atggttggat
     1981 cagcaacaac aaaattgaat ttgattagca atcatgttaa actgaac
//
LOCUS       DAZKAS010000245         2027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000245 DAZKAS010000000
VERSION     DAZKAS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.252"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..168
                     /gene="mobC"
                     /locus_tag="ACG8H7_002831"
     CDS             <1..168
                     /gene="mobC"
                     /locus_tag="ACG8H7_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFP8984874.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVDPGDAQRLSENLKIMQ
                     KELQKIWQQLK"
     gene            150..1064
                     /locus_tag="ACG8H7_002832"
     CDS             150..1064
                     /locus_tag="ACG8H7_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP8984875.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            1024..1302
                     /locus_tag="ACG8H7_002833"
     CDS             1024..1302
                     /locus_tag="ACG8H7_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984876.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            1309..1998
                     /locus_tag="ACG8H7_002834"
     CDS             1309..1998
                     /locus_tag="ACG8H7_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984877.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
BASE COUNT          802 a          384 c          374 g          467 t
ORIGIN      
        1 caggcaatcg cgcgcgattt agccaaagct ggcgggaata tcaatcaaat tgccaagtgg
       61 tgcaacacac accaacacga cgttgatcct ggcgacgccc aacgcttatc cgaaaaccta
      121 aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagc cgaaccactt
      181 cttgtagccg agcgatcaat tacgccgaac cacgagcaac cgtcaaaaca gggataaact
      241 gtgacattga ttatgccaaa agcgaaatga aacaaattcg catgctgtat ggaaaagacg
      301 atcgtgtaca agcacactta ttgatccagt cttttcgacc aggagaaatt accgctgaaa
      361 aagccaatca actaggggca gagtatgcga aaaaaatagc ccctgaccac caaatcgcga
      421 tttacacaca tacagacaaa gaccatattc ataaccacat tgtcattaac tccgtcaatc
      481 tggaaactgg gaacaaattt caagcccatg gtcaagcatt tttaaacaaa tgttatgacc
      541 taaatgacga gatttgtctt gaacatgact taacaatcac tgaacgaggg aagaaacccg
      601 aaaaacgaac catgtctgaa atcaaattaa aagaaaaaaa tgaacctgtt tggaaagacg
      661 aaattcggtt tgcgattgat caaacaatga aaaacaaaaa aactcgaacg tataaccaat
      721 tttgtgattc tcttaaaaca ttcggcattc attgcttgaa tcgtgggaaa aattttactt
      781 acgaattgat ccacaaaaag aaaaaagtac gatccaacaa actaggaaag gattacgaaa
      841 aggagacgat tttacatgag ctggacagac gagaacgagc ctacaatcaa caacgaacca
      901 acgagctacg aagagctcga gctagcattc gcgaaactgc aaaagaagta cgaagagaac
      961 aacgaactct tacgactgaa caaccggaaa ttagaccgac tcgaagaaac catgaccaac
     1021 acgttgaaag aggagcagac taccaacaag aattatcgtt ctaaaacgtt acaagacata
     1081 gaaaatgaaa taaagaaaca acgtgctttt cttgcacctt ataccaaaga aagaatacaa
     1141 actacaattg attcacaagt caaaaaattc caccatgcgc tattaaacca gcgaaataaa
     1201 gaacagaaaa aagtaattat gcagcgatca atggatattc ttgccccctt atgttgctta
     1261 atcatgacac tgttagcctt ttggctggta tggggagaat aaatagaaat ggcattctat
     1321 ttaccctatc tactcatttt tgccagtatt ttaggaatta tttggcttag ctataaggct
     1381 tatcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagaggtt tttgttgcca
     1441 ggggttatat ttacattatc aatcgtttta agtggtttat tcggcgtttt agagggaaat
     1501 aaacgtattt caagaagtat tttattaggg gatatcactc aaaagtatga agcagcatgt
     1561 gataaaaaga aaaaggagca agtgttaaca caaaaaatgg aagagtttac cacttgttat
     1621 gaggaaatga acgatatttt tgtgaatcaa gaaaaacgtt tgacggataa aaacatggaa
     1681 aaactcactc gtctttatca aaacttgcca gaaaaacagc aaaaagaggt tcaagacaac
     1741 tatgagcgaa ccaaaaaaga cgtgcaatat tttaaagata cgcagacaga agaagcttgt
     1801 tataatttat tcagtgatac aatccctttt tcaactagtg aacaggaacg aaaagaacgg
     1861 caacaatcag ttacctacga acgttataaa gcactgcttc agcaagcaac gaacatacaa
     1921 aatccaacga aaaaagaaac ggcattaaat tatctaaaaa gtgtcaagga atggttagat
     1981 cagcaacagc aaaattgaat ttgatcggca atcatgttaa actaaac
//
LOCUS       DAZKAS010000246         1992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000246 DAZKAS010000000
VERSION     DAZKAS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.253"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8H7_002835"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8H7_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFP8984878.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8H7_002836"
     CDS             852..1040
                     /locus_tag="ACG8H7_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFP8984879.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8H7_002837"
     CDS             1053..1610
                     /locus_tag="ACG8H7_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFP8984880.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8H7_002838"
     CDS             1642..1884
                     /locus_tag="ACG8H7_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP8984881.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          554 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ct
//
LOCUS       DAZKAS010000247         1989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000247 DAZKAS010000000
VERSION     DAZKAS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.254"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..446
                     /locus_tag="ACG8H7_002839"
     CDS             <1..446
                     /locus_tag="ACG8H7_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984882.1"
                     /translation="GNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKC
                     GNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVE
                     DAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(507..872)
                     /locus_tag="ACG8H7_002840"
     CDS             complement(507..872)
                     /locus_tag="ACG8H7_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFP8984883.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(948..1193)
                     /locus_tag="ACG8H7_002841"
     CDS             complement(948..1193)
                     /locus_tag="ACG8H7_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8984884.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1263..1517)
                     /locus_tag="ACG8H7_002842"
     CDS             complement(1263..1517)
                     /locus_tag="ACG8H7_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984885.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1681..1986)
                     /locus_tag="ACG8H7_002843"
     CDS             complement(1681..1986)
                     /locus_tag="ACG8H7_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984886.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          733 a          347 c          358 g          551 t
ORIGIN      
        1 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
       61 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
      121 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
      181 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
      241 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
      301 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
      361 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
      421 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
      481 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
      541 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
      601 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
      661 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
      721 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
      781 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
      841 tttgaaatca atttttcatg ccataatcgc atagaaatgt ctcctttata tatagattgt
      901 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
      961 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     1021 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     1081 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     1141 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     1201 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     1261 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     1321 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     1381 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     1441 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     1501 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     1561 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     1621 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     1681 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     1741 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     1801 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     1861 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     1921 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     1981 tggcaaaat
//
LOCUS       DAZKAS010000248         1953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000248 DAZKAS010000000
VERSION     DAZKAS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.255"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..1567
                     /locus_tag="ACG8H7_002844"
     CDS             101..1567
                     /locus_tag="ACG8H7_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP8984887.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          608 a          334 c          397 g          614 t
ORIGIN      
        1 aaaagaatac acacataaaa taacactgtc ttgtaagttt gttaaaaatt gaatatgatt
       61 acatctggat aacaaatttt tatcataaag gagagaacga atgaacaaca aacaattacg
      121 ctggtttact gttgcgttaa ttgcatttaa catggtttgg ggcatgggga atgtagttaa
      181 taactatgct cagcaaggag tatcagttgt cacctcttgg ctattgattt tggccattta
      241 ttttattcca tacgcattga tcgttggaca attaggatca gccttcaagg atagtaaagg
      301 aggcgtcagt tcatgggtcg aaaatacgac atctaataaa cgactggcat actatgccgc
      361 ttggacttac tgggtcgtcc atattcctta tttggcacaa aagcctcaag caattctgat
      421 tgctggtggt tgggcagtcc aaggaaatgg aaatatcgta aatacgatgt cagtacaaat
      481 ggtagcagtt atttcactta ttatatttct tgccttttta tatttatcaa caaaaggact
      541 ttctactttg aaagtgattg gcggactggc cggaacagcg atgttcgtca tgtcgatctt
      601 atttattctt ttagctgtaa cggcaccgtt catcaatcca acgatggaat ttgccacacc
      661 acatatgaat caagtgaaaa catatattcc aacatttgat ttcagttatt tcacaaccgt
      721 ttcgatgcta gtatttgcag ttggaggggc ggaaaaaata tccccttatg tcaactctac
      781 aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc ttagcagcaa tggtagggat
      841 ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt gctagtgata atattcctaa
      901 agacttgatg gcaaatgggg catacagtgc ttttcaaatt ttaggtaatt actatggtat
      961 cggtaactca ttgatgatca tttacgcatt gacaaacatg attggacaaa tctccgcatt
     1021 agccttttca attgatgcac cactaaaaat cttgttagca gatgcggatc cagaatttgt
     1081 accaaactgg ctacgtaaac gtaatcgtaa aggtacattg gtgaatggtt atcttctgac
     1141 aggtatacta gtaggaatca ttattttgtt gccgattttt ggtatcaaag atatgaatga
     1201 attagtaaaa tggttgacga acttgaactc agttgtcatg ccaatgcgtt acctatgggt
     1261 gttcttggca tatatgatgt tgaataaagc ttataagaaa ttcaattctg aatataaatt
     1321 tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg tgctttgcct tcactgcgtt
     1381 tgcatgtatc ctaggaatgg tgccaaaagt agattatact gctgatccta aagcatggtg
     1441 gttccaatta tttacgaata tcatcacacc aatcgtcttg atcttattag gtatgatctt
     1501 gccagcaatt gctcgtcgag acaaaaacaa aaaaatcact gaaccagaaa cagtggttca
     1561 gccttaaaat agaaatactt gttacaagag gttgtgacag aagtggtcag cttcaagaaa
     1621 taagaaggaa tttacgaaaa ttgcttctca aatttttgtg aagtttcggc ttgtttttcg
     1681 aagaagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgtc ctgcatcaag
     1741 taataagaac agccaaacaa aaatagcccc tcatattttt cgtttggctg ggcttattac
     1801 cgcagatgca agctttggaa caccgtttaa ggaagaggtt gtaaaatcag cgatttgacc
     1861 tgagtattag agaaacaaac ggaaaaatag ctcctcatat tttgccgctt tttgagctaa
     1921 tgctgaaggt cagctgattg aacaccattt att
//
LOCUS       DAZKAS010000249         1950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000249 DAZKAS010000000
VERSION     DAZKAS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.256"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..300)
                     /locus_tag="ACG8H7_002845"
     CDS             complement(136..300)
                     /locus_tag="ACG8H7_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984888.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            595..1923
                     /locus_tag="ACG8H7_002846"
     CDS             595..1923
                     /locus_tag="ACG8H7_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFP8984889.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT          655 a          320 c          384 g          591 t
ORIGIN      
        1 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
       61 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
      121 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
      181 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
      241 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
      301 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
      361 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
      421 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
      481 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
      541 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
      601 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
      661 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
      721 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
      781 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
      841 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
      901 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
      961 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     1021 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     1081 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     1141 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     1201 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     1261 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     1321 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     1381 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     1441 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     1501 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     1561 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     1621 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     1681 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     1741 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     1801 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     1861 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     1921 tagacgagta tttcaatgaa gagtatgtag
//
LOCUS       DAZKAS010000250         1947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000250 DAZKAS010000000
VERSION     DAZKAS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.257"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..1226
                     /locus_tag="ACG8H7_002847"
     CDS             57..1226
                     /locus_tag="ACG8H7_002847"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFP8984890.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1493..>1947)
                     /locus_tag="ACG8H7_002848"
     CDS             complement(1493..>1947)
                     /locus_tag="ACG8H7_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984891.1"
                     /translation="EMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTE
                     DGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVT
                     VKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          686 a          331 c          298 g          632 t
ORIGIN      
        1 aagctatttt agctttattc tttctgtaaa aaagaaagga gactaaataa actattatga
       61 aaaaaggaaa acatgctaaa aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt
      121 ttagccttag tgcgttctct acaggaacta ttcttggatg gcaattttac aataatgaac
      181 actctcagat agttgcccat gctcaatcag ctcaaaaaaa gttgatactg actaccaatg
      241 aacaacagct cgttgaaaaa atcgatcaac tattaagaaa aaatgattat attggctcaa
      301 tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg ctatggctat gcgaataaac
      361 tcacgcaaca accaaatgat ccttctcttt attatcagat tggttcggtt caaaaagcat
      421 tgactactct tttgatttta aaacaagtcg agcgtgggca cctttcttta gatactaagc
      481 tggctcaatt ctatccacaa attgctggca gccagcaaat tactatccgt gacttgctgt
      541 atatgcgctc aggcttgcac cgaactgctt ctccaaccat accgatgact gatgaagaag
      601 tcgttcaatt tgcgattcag cacttaaaat ttacagatta tcagacctct cattatgatt
      661 cattgaactt taccatattg actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa
      721 aattattgac cgatgatatc atcgaacctt taggattaca gcatacaaaa ttttatgacc
      781 aagtcaaaaa ttcatccaat catgcactat cctatcaaat gtctaaagag gatgattact
      841 ttaaaccact gacagaatct gaaacagaga tccataacga attagggact ggtaatatca
      901 gtatgtccgt ctatgattta aatacgctgt ttacacaagt tttatctgga aaactagttt
      961 ccaaagactt actcttctca ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg
     1021 gggtatatag cggagataat tatatcctgt cgcaaggaaa tatcgactct tttgatacat
     1081 ctgctatatt tagcaaagat tcacaggatg ctatcatcat ggaaagcaat ataaaagctg
     1141 acaaaactat caaactgcct actgctgatc taagaaacca aatctatgac ctgatggaag
     1201 gaactactaa gttggcaaat agctgatatg atgaaaagat agcaaatact acttctttga
     1261 atagcatttg cttaaaaaaa gtactcgact aaacgtagga aagagtgtgg gacaaatcta
     1321 aaaatcaatt tttgctacag actttaattc caaataaaca gaacagaaag aggtgttctt
     1381 ctttctgttc tgtccaaatt attctaagat ttctctttac caacattatt tgacaaaggg
     1441 cctattaaat ctaaaatagt atctttagat tgctaaaata tgtgctagca aatcattcat
     1501 ggcttgttct tatttttaat tttatgcaat aaaaaagaag aacaattagc gtgattatag
     1561 aaataaataa tgagatttta gaaaaaaatg tgtatgaata cctgattgtt actttattta
     1621 ttccactttc actttttact gtaacaaaag gacctccttg ttttagtact gtagcatttt
     1681 ttgaattaac agtaaccgtt aatcctttat accggaatac aggtaaaact attgttttgc
     1741 cttttgaaga tttaaacgta aatgtaattt catctttatt tggagaaaag ctactataaa
     1801 taacttgacc atcttctgta acttgatggt ttttcaaaag caattgatct tttttatagc
     1861 ctataggtac ataatcaaaa ttctcaaatt cttcaagttt attttccatt tgttcattag
     1921 aataagttcc ttgaaaattc atttctt
//
LOCUS       DAZKAS010000251         1892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000251 DAZKAS010000000
VERSION     DAZKAS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.258"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..477
                     /locus_tag="ACG8H7_002849"
     CDS             139..477
                     /locus_tag="ACG8H7_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984892.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="ACG8H7_002850"
     CDS             complement(682..1257)
                     /locus_tag="ACG8H7_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFP8984893.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          585 a          398 c          318 g          591 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc ga
//
LOCUS       DAZKAS010000252         1803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000252 DAZKAS010000000
VERSION     DAZKAS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.259"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1107)
                     /locus_tag="ACG8H7_002851"
     CDS             complement(67..1107)
                     /locus_tag="ACG8H7_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFP8984894.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          619 a          278 c          220 g          686 t
ORIGIN      
        1 gaataataaa aaatttttaa ttttaatgtt actccatgaa gcaacaatca ctttgtcagt
       61 aaagtttcac tctaaccagt ctttcgtaat agtgcttggg gagctttcat aattcaaacg
      121 tttttcactt gatactttag cagctattgg tttttcgata aaccaatttt ttacatagac
      181 aaaaatcagg ttctggatat tttcgacttt ttcggttttt aatttttgat aggcttttaa
      241 cagtgtcgtg tataattctt gttctgcatt gacataataa ctatcgagtt cttcaaaaac
      301 tatcgaaata ccactttcta tttcagcttt tttctttgca ttatgaatag tctggacggt
      361 tttttgagct tcactgtaac ttgaagaaaa tgtttttatc aaatttaata ctcgttcagg
      421 gacaaacgta gcaatgctgt cgtttgtcat aattgtctca aaattgtcta acaacaattg
      481 atcttgtagg cggtcttttt cagtgtctat attgtgtcta ttagtgtcta atatattatt
      541 attaagttct ggtatgattt tcataccctc tgatttatca acgttttggc acggtatgat
      601 tttcataccc tcttgagccc caagggattc agcgtctgat ttttcgtcga gggtgggtat
      661 gattttcata ccctctgatt tatcaacgtt ttccctattc tctctatcaa atttttcgag
      721 taagtagata tcattttctg agacttttaa ttctgctaga tatagtctat taggattatt
      781 tttccctaac tgtttgttaa atcccatctg cttttgtaat aataaaccaa aggattccaa
      841 ctcttttttg attgctatcg ctttttgttt ttggcagttt aatactctac atagttcatt
      901 tgtcgtataa gtaaaataaa cattaccttc ttcatcgatt tgccgtttgc tgattgcgat
      961 ctctaatctt gcttttaaaa gcatataggc aatcttagtt gaatctttca tttttttgta
     1021 ttcgtcagaa acaataaaga cttttggaaa ttgataaaat aattcgttgt aaactctatc
     1081 cgccttaaaa aatttaaaat cagtcattgt agatcctcta ctttcacgtt gttttattta
     1141 tgtaaaatta cctactcttt tcgaaaatag caaagataaa taaaaaatgc aaacgcatta
     1201 aaactgtttt ttaattaaat actacctaaa atcagaaata aaatgaatta tgtactacct
     1261 ttaaagtcat taagaactac tttaaataaa tattatttat aattaaatac taccagaaaa
     1321 acgtatgaat aaaatttagt tttaaaatta aagataatta agtactacca gtgtcaaaac
     1381 aaaaaataaa ttaagaacta ccttatgcat ataaaacata ctaccattac tagtaatata
     1441 tactacctta tttcagtagt atatattata ttattcttat aagacaaggc tttaggctag
     1501 aaaacaatag attatatact actaaaaaat acaataagta ctaccaatgt cagatatata
     1561 taataattat gtactaccat taaaattaac ttatttaata agatactact gtgttaatct
     1621 gaaaaaaatt actttaaagt agttacatac tacctgttaa tttagatact accattaata
     1681 cttaaattag atactaccta tatttatatt aagtactacc gttctaagaa taagataata
     1741 aaaaaatact accaaattgc aattaaatac tacttaaaat gtaaaaaata agaactaccc
     1801 aaa
//
LOCUS       DAZKAS010000253         1753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000253 DAZKAS010000000
VERSION     DAZKAS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.261"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..1630
                     /gene="guaB"
                     /locus_tag="ACG8H7_002852"
     CDS             146..1630
                     /gene="guaB"
                     /locus_tag="ACG8H7_002852"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP8984895.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          614 a          304 c          401 g          434 t
ORIGIN      
        1 acaatctctt ttaatataca cagatgtaaa cgctttatat tggtattcta tgagaaagct
       61 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      121 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      181 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      241 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      301 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      361 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      421 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      481 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      541 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      601 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      661 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      721 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      781 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      841 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
      901 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
      961 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1021 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1081 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1141 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1201 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1261 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1321 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1381 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1441 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1501 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1561 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1621 tgaatcttaa aaacattaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
     1681 ctaaacacgt ataaacggcg gaagtagaag caactccttc ggaaataaga agaaattcac
     1741 aaaaatttga aaa
//
LOCUS       DAZKAS010000254         1738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000254 DAZKAS010000000
VERSION     DAZKAS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.262"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8H7_002853"
                     /pseudo
     CDS             1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8H7_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1218..1610
                     /locus_tag="ACG8H7_002854"
     CDS             1218..1610
                     /locus_tag="ACG8H7_002854"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8984896.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          715 a          133 c          297 g          593 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttattgatga taatatggtt tttgatattg
     1261 ataatttaaa aggatttctt aatgatacca gttcatttgg gtttatagct aaagaaaata
     1321 ataaaattat aggatttgca tattgctata cacttttaag acctgatgga aaaacaatgt
     1381 tttatttaca ctcaatagga atgttaccta actatcaaga caaaggttat ggttcaaaat
     1441 tattatcttt tattaaggaa tattctaaag agattggttg ttctgaaatg tttttaataa
     1501 ctgataaagg taatcctaga gcttgccatg tatatgaaaa attaggtggt aaaaatgatt
     1561 ataaagatga aatagtatat gtatatgatt atgaaaaagg tgataaataa atgaatatag
     1621 ttgaaaatga aatatgtata agaactttaa tagatgatga ttttcctttg atgttaaaat
     1681 ggttaactga tgaaagagta ttagaatttt atgatggtag agataaaaaa tatacatt
//
LOCUS       DAZKAS010000255         1733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000255 DAZKAS010000000
VERSION     DAZKAS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.263"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..520
                     /locus_tag="ACG8H7_002855"
     CDS             <1..520
                     /locus_tag="ACG8H7_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984897.1"
                     /translation="KLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKV
                     NSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISF
                     SPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENW
                     AELRKLVEYGII"
     gene            507..>1733
                     /locus_tag="ACG8H7_002856"
     CDS             507..>1733
                     /locus_tag="ACG8H7_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFP8984898.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKK"
BASE COUNT          563 a          225 c          275 g          670 t
ORIGIN      
        1 taaattgatg aaaaacatac aatcaatctt tgggatagaa gaagtttttg tcggagataa
       61 atgtatcttt tttgatcaac ctttattatc tgatggttat ggcatagatg aaacagatat
      121 tttcgctaaa gtaaatagta tattttctgg aaaaaaagaa actttaatta agcttcatcc
      181 tagagcaagg acaaatcgtt atggtgaaaa aaacatattg aattccagtt taccgtttga
      241 agtcttgcta cttgaaaatg acttctcttc aacacttttg attagtccta tatctacaat
      301 ttctttttca ccatctatga tgtttgaaaa gacgatcaac tctattatgt tggctaaatt
      361 aattttaaat gaatatacag cattatctga agataaaaaa gaaacattga caaatattgt
      421 tcttttctgt gaacattata accgtctatc agaagaaaaa attttactcc cagaaaactg
      481 ggcagaatta agaaagttgg tagagtatgg gattatttaa cacactttcc aagtcaaaat
      541 tagttaagtc cagtttctgg tatactatag ggaatttttt tattaaggga atttcattca
      601 taactattcc tatttttgta aatattacta ctcaagcaga atatggtttg attaataatt
      661 tcacttcatt tgtatcaatt ttttcgattc ttgttgggct aagcctaaat tcggcaatta
      721 ataatgcaag ttttgactat aaagatgaca taaatgaatt tatgtcatcg gtactttctc
      781 tagctacact ttcgttaatt gtgttcttat taatagggaa catatatttt ttgttttcaa
      841 atgctttttt tgatatgaac caacttgtat ttaatttgat gttattacaa agttttgcta
      901 gttttgtaat tagttatatc cagtcttatt ttacgattaa ctttaaccat tttaaatatt
      961 tgattttatc ttttatatct acttttttga atattgcaat ctctgtagct ttgatgcaga
     1021 cattatattt tgatgataaa tatgttggca gagtagtcgg cggaacactg tcatttgtgt
     1081 tgataggttt agtaatttat ttaagaataa tgataaaggg tgctcatttt tttgataaaa
     1141 aacagtggag atacgcttta aaaatctctt tgccgctaat cccacattcc ttagcaaata
     1201 tagttttagc acaattcgat agaattatga ttaattctta tttaggtcct gctgcggggg
     1261 gaatatattc ctacattagt aacataggta ttattctaag cgtaatatgg atgtctacta
     1321 ataacgcctg ggttccatgg ttctatggtg agatgagtaa aaataatgaa aaaaaagtga
     1381 agaaagttag taactattat ttagtagtct ttactttgat tacgatgatt gtaatggtag
     1441 tttcgattga tttagcaaaa tttatggctc cgaaagacta ttattctggg ttatctttag
     1501 tggttccgat aaccttaggc tatttctttc agtttttata tagtttacca gttaatgctg
     1561 aattttatga gaaaaaaaca tcgtttattg cgattggtac agtagcaagt gctatagtta
     1621 atatactatt aaacatgatt ttcataccta ggttaggaat aattgctgct ggatacacaa
     1681 ccgtatttac gtatttttta ctttttattt ttcattataa tatcgctaaa aaa
//
LOCUS       DAZKAS010000256         1721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000256 DAZKAS010000000
VERSION     DAZKAS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.264"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..963
                     /locus_tag="ACG8H7_002857"
     CDS             130..963
                     /locus_tag="ACG8H7_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP8984899.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            979..1716
                     /locus_tag="ACG8H7_002858"
     CDS             979..1716
                     /locus_tag="ACG8H7_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFP8984900.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT          574 a          256 c          379 g          512 t
ORIGIN      
        1 agtccattcc ctgtttttta tggtccctgt gctataattc taaataaagg ataaggattg
       61 gaaataatcc ttttatcatt aaaaatatga tgacgaagta gaataaaatt gggagtaggg
      121 gggaaagtca tggatatcca aactgtttta tctgatctga aattaaatgg taaagtgatt
      181 ccagttgttg gtggcgatgt taatcagacc tatcgtataa aaaccgaaca tcgtgcttat
      241 tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg aagcagaagt agacggatta
      301 aaagaactgt cagcattcgt acgtgtaccg gatacgtata tgctaggtga aacaagtgaa
      361 ggcgcttatt tgttaatgga atggatcgag ccgggaaaag gggatcaacg agatcttgct
      421 gcagctttag ctaatctcca tcagcaaaca gcgccacaat ttggttttcg taaagataac
      481 tatttgggta cacttgttca gaaaaattct tttgaagaag attggtggac ttttttcttt
      541 aaagatcggc ttgaatccca gatttctttg gcagaagaaa cgaatcgatg gaatgtacag
      601 cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga aatcggttga accaaaaaag
      661 atcactccac gcctcttgca cggagacctg tggagtggta atgtgttctt tgaccagcaa
      721 gggcatccag tttttgtcga cccagctgtt tcttatggga atcgggaaca agatattgca
      781 atgagccagt tattcggtgg tttccgaccg gaatttttag atgcttatca aactatattt
      841 ccattagaaa agggatggaa agaccgactg ccgatttacc agttatatta tcttttggca
      901 catttgaata tgtttggaga aagttacggt tcccaagtag atcaattact agaaaatttt
      961 taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa agcatgaagt ttcatattat
     1021 gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt taggagtagt tatcaaaacg
     1081 tctgaagatc aaagcgacca gttgggaaga ggcagtgaat tcgtcaatag ctttggactt
     1141 acttgggtca tcacgaatta ttccatggag attaatcgtc tccctagagt cggtgaagta
     1201 atctcagtaa cgactcaagc catggagtac aataagtttt tctgttatcg gaacttctgg
     1261 atccatgatg aagatgggaa agaattagtc aaaatagaat ctgttttcgt attgatggat
     1321 tttgtgaatc gaaaaatgag cagtgttaat gaagagatca ttgctccttt tgagagtgag
     1381 aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga ttgattcggg cttcatgttg
     1441 ccatatcgtg ttcgttttta tgatatcgat agtaatcagc atgttaataa tgcgatgtat
     1501 tttaattgga tcattgacgt attaggatat gactttttga ctacgcatgt tccagaaaaa
     1561 gtaattattc gatttgataa agaagtagag tacgggaatg agatcgaaag ccattttgaa
     1621 caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa tccgaatgca agaacagctg
     1681 tattgtgaag caaatattat atggaaaaat aaataaaaaa g
//
LOCUS       DAZKAS010000257         1717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000257 DAZKAS010000000
VERSION     DAZKAS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.265"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1509)
                     /locus_tag="ACG8H7_002859"
     CDS             complement(28..1509)
                     /locus_tag="ACG8H7_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984901.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          530 a          232 c          299 g          656 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
     1621 gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg gtattttgtc
     1681 aagatattct tgtttttaag gcggaaaaat tgatttt
//
LOCUS       DAZKAS010000258         1712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000258 DAZKAS010000000
VERSION     DAZKAS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.266"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8H7_002860"
     CDS             <1..156
                     /locus_tag="ACG8H7_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP8984902.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="ACG8H7_002861"
     CDS             175..1452
                     /locus_tag="ACG8H7_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP8984903.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..>1712
                     /locus_tag="ACG8H7_002862"
     CDS             1462..>1712
                     /locus_tag="ACG8H7_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8984904.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNIS"
BASE COUNT          717 a          149 c          288 g          558 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct tt
//
LOCUS       DAZKAS010000259         1674 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000259 DAZKAS010000000
VERSION     DAZKAS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.267"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACG8H7_002863"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACG8H7_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFP8984905.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          463 a          291 c          331 g          589 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactggctt ttgaacaccg
     1501 tttattgagc gtctataaac tgacctgagt attagagaaa caaaacggca aagtagctcc
     1561 atatactttg ccgttttgtt ttgaactaat accgaagaga agctgatcaa acaacatttc
     1621 ttttataata tagattttag acatatatat gatataaaaa tagttttcgc aaaa
//
LOCUS       DAZKAS010000260         1646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000260 DAZKAS010000000
VERSION     DAZKAS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.268"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1551)
                     /locus_tag="ACG8H7_002864"
     CDS             complement(73..1551)
                     /locus_tag="ACG8H7_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984906.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          527 a          299 c          227 g          593 t
ORIGIN      
        1 tagttacctt ttctttgaaa aaaacataaa tcagctaata aatatgtgag acaaagctgc
       61 catccataaa tactagctga ttttttcagt taaatgtcta tttttttctc tgatgatggt
      121 atttccaaaa tattcccagt catctggagt cggtggattt ttccaaatta tctgttcttt
      181 attaaatttt tcaagtaaac aatcagataa ataaatattc gtacgagaag tgattcgact
      241 ctcaatcaca atattgtaat ttttaaatcc tttcacttga gaaataatgt aatcggtata
      301 agcagatcct tgcgaaaaat caatacagac atgaatcggt gcttcaaaat atgataccgg
      361 acaaataccg accaataaaa agagataatc ataaaataag cgaatcatga aacgttcatc
      421 ttttcccaag aaatagcgtt tattctcttg tatgaccttt tggattgctg tactgaatat
      481 tggatacgtc tcgaagaaaa actgtagctg ctttttagac gtaaagctag tcatctcaaa
      541 gtcgaatatc caattttttc gattgacatg catcataccc atacgaagtt cttttcgtat
      601 atatagtctt tcttcttcag agatttcaat cgaaaatggt aattgatcaa tcaattccac
      661 tgacattttt tgactaatca tgtggatatc atgaaaatga gcttccgcaa gtccaaatgc
      721 gccatctaat tcaaattctt cttccgtgat caaaaacaac atgatataag aaatctcatc
      781 aatgatctgc tctttaggga tatgaagaaa agaaatatgt gataaatcct tgatgatctt
      841 cattccttct gagctcttaa agtattcatc tgaaaagaaa cgttttccga ttggttgatt
      901 cccataggcc ctcaacaaac taatagtggt tagcaaattg aatttgattc gttttgttgc
      961 agacagtttt aaattgaaca gatattcaaa atattgcacg atactctttg cttcattcat
     1021 gacgctttct ctaaatggca tagaaaaacc attaaagacc ccgaaatata ccccaaataa
     1081 tacgttccgt atgtttattt cttccccaac taatatgttt ttctttaact ttatttgatg
     1141 ctgttcaaga aaagagacta attctcttct ttttacatag gctcttgata tgcttaaaaa
     1201 atgattctct gcaaattttt cgatcgttac ttttgaatcc acgatctccg ttaacagtcg
     1261 agcaattttt gatcgttgaa agtagattct tcttaaattt ttgatatccg tcaaattcag
     1321 acgttcgaca tggatctctg cttcttctga aatttggatc tctggctttg ttttgaattc
     1381 agtaaattct ttatttaatt ctatgatata atttcttgtt ttaaatttag acaaacctaa
     1441 tgtttcatat aacttaggca gcactacata attctttttc tcaaattcta aatatctagc
     1501 tagcagcagt ttttctctat cttcctcttc tataaggtaa tccaacttca tatcctacac
     1561 ctcaaaaaat gcctcatacc aattacttga tatgcagatg aaagttcatc ttttcaccta
     1621 tactgatttt atgatggatt atgctt
//
LOCUS       DAZKAS010000261         1563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000261 DAZKAS010000000
VERSION     DAZKAS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.269"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..257
                     /locus_tag="ACG8H7_002865"
     CDS             <1..257
                     /locus_tag="ACG8H7_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFP8984907.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
     gene            349..564
                     /locus_tag="ACG8H7_002866"
     CDS             349..564
                     /locus_tag="ACG8H7_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFP8984908.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            581..853
                     /locus_tag="ACG8H7_002867"
     CDS             581..853
                     /locus_tag="ACG8H7_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP8984909.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            855..>1563
                     /locus_tag="ACG8H7_002868"
     CDS             855..>1563
                     /locus_tag="ACG8H7_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP8984910.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSG"
BASE COUNT          624 a          234 c          295 g          410 t
ORIGIN      
        1 tgatcggttt tgttccttat ttagttgata cggacagcgc aacgataaaa tcaaacctgg
       61 aagaactgta taagcaacat aaagaagata acttggtttt ccaaaatatt atcaagcgaa
      121 gtaataaagt aagtacctgg tctaaaaacg gcattacaga gcataaaggc tatgacaaaa
      181 aagttttatc catgtataag aacgtatttt ttgaaatgct tgagcgaatt attcaattag
      241 aaaacgaaaa agaatagaat cacaaatcac aagtgattaa tcacaaatca cttgtgattt
      301 gtgattgttg atgataaaat aagagtaaga agaaatagaa agaagtgagt gattgtggga
      361 aatttaggcg cacaaaaagc aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata
      421 atgggagata agacggttcg tgttcgtgct gacttgcacc atatcataaa aatcgaaaca
      481 gcaaagaatg gcggaaacgt aaaagaagtt atggatcaag ccttagaaga atatatacgg
      541 aaatatttac ctgacaaact ttaaaaggag tgaaaatgaa atggcagtta cgtatgaaaa
      601 aacatttgaa atagagatca ttaacgaatt atcggcaagc gtttataatc gagtattaaa
      661 ctatgttttg aaccatgaat taaataaaaa tgactctcaa ttattggaag tcaatttatt
      721 aaaccaatta aagcttgcaa aacgtgtaaa tctttttgat tattctttag aagaattaca
      781 agccgttcat gagtattggc ggtcaatgaa tcgttactca aaacaagttt tgaataaaga
      841 gaaagtggct taatatggca aatatagtca attttactga caaacaattt gagaatcgct
      901 taaatgataa tttagaagaa ttgattcaag gaaaaaaagc ggttgaatcg ccaaccgctt
      961 ttttacttgg tgggcaacca gggtcaggga aaaccagttt gcgatcagca atttttgaag
     1021 aaacacaagg gaatgttatt gtcattgata atgatacctt taaacaacag caccctaatt
     1081 ttgatgaact agtgaaactt tatgaaaaag acgtagtaaa acacgttacc ccttattcta
     1141 atcgcatgac agaagcgatc ataagccgtt tgagcgatca agggtataat ttggtgatcg
     1201 aaggtacagg acgaacaaca gacgttccta ttcaaaccgc aacaatgctt caagccaaag
     1261 gttatgaaac aaaaatgtat gtcatggcag tacctaaaat caactcatat ttaggaacaa
     1321 ttgaacgata tgaaaccatg tatgcagatg atccaatgac agccagggca acaccaaaac
     1381 aagcgcatga tattgttgtc aaaaacttac cgaccaattt agaaaccctt cataaaacgg
     1441 gcttatttag cgatataagg ctatataaca gagaaggagt aaaactctat tcaagtttgg
     1501 aaacaccttc tattagtcca aaagaaacct tagaaaaaga attgaatcgt aaagtatcag
     1561 gga
//
LOCUS       DAZKAS010000262         1551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000262 DAZKAS010000000
VERSION     DAZKAS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.270"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8H7_002869"
     CDS             132..344
                     /locus_tag="ACG8H7_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984911.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACG8H7_002870"
     CDS             506..1024
                     /locus_tag="ACG8H7_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP8984912.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACG8H7_002871"
     CDS             1031..1543
                     /locus_tag="ACG8H7_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984913.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          241 g          518 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DAZKAS010000263         1548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000263 DAZKAS010000000
VERSION     DAZKAS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.271"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(141..1433)
                     /locus_tag="ACG8H7_002872"
     CDS             complement(141..1433)
                     /locus_tag="ACG8H7_002872"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP8984914.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          434 a          336 c          270 g          508 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacg
//
LOCUS       DAZKAS010000264         1532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000264 DAZKAS010000000
VERSION     DAZKAS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.272"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..792)
                     /locus_tag="ACG8H7_002873"
     CDS             complement(<1..792)
                     /locus_tag="ACG8H7_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8984915.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT          504 a          297 c          255 g          476 t
ORIGIN      
        1 atacaaccaa caaaactcct cttctgataa agcaacttga taatttgttt gaatcgcttc
       61 tttcaaggaa tacattaagc gaaaattctc actagcaatt actcgctcaa tcagaccttg
      121 aggaatggct atcgtttttc cttgtctcac acgctcaatc gcaatataca aactataata
      181 aaattcagca ggagaagtcc ctgttccaat cgagaaatac tcaaatttat catagaatgt
      241 agcttgcaca agttcatgaa gatcccttgg tggaacaaga gtatggggcg tcatctcccc
      301 ctcataatag aagtctttaa aaaacttgcg aatatttgcc tcttctcctt ttaaatcaag
      361 aggataaaga ctcaattcta agccgtattc ttccaaaact ggttgaatct gcttcaaata
      421 ccgacgaaaa gtcgcttcag ataaatgaaa acgatcaatt agctccgtca tatcttccaa
      481 ttcgccataa aagacattcc ctaacaactc aaataaaatt tcgttctcca gtagttgctc
      541 cttctgcttg agaaaaagaa ccggtctttc cttctgaaaa gaatacccac ttttcgttga
      601 aacaaattga acactttcag aaaagtagtc cttcatttta ttgacatctg tttgaatggt
      661 tcgttcagat acatgattac tctttgctaa ttctcctttt gtggcaagtt gattgcgctc
      721 aaattgattg ataatccgaa accagcgtac aatcgatgga tcaacaataa attttaactg
      781 taaatttttc atcaacaacc ctcctcttag aaaaaaattt catcctatac ctatataatt
      841 acgataccat tttttctcct ttataggtaa tgttttgggg aaggaaaaca agtttctatt
      901 actcacctga taaaatcaat accgtcgact atactgcgga attgtttggg tatagcaaat
      961 aagaccaaat gaaaaaactt ggtcttggat aaaaaatgta accgatttct atttacccaa
     1021 aaagaggata tgctatactt aatgagacaa cttactatca ttcaggtgta gtattgttcg
     1081 gtcgtggtac cccaacaact ttacaatttc ttttgaaacg ttggttgtga ggagggttgc
     1141 caatgaatca cagtggaatg ggaaaggtta cgttctgaaa tagaatgttg caaaatattc
     1201 tagcaatctt atcgatcatt aacttgatct tagagattgc gaacaaagta aaaagcttaa
     1261 aggactagtc ctttaagcca cactcattac cactgccgaa gcagtttcgt aaaaggtgat
     1321 accagcacct tttacctaca cctgaattat agcaagtttt ttggggttag tcaaaatagc
     1381 taataatggt gtagtgcaat ttctttttga aatgttggtt gtatgctaat gaatcccaat
     1441 gaactgggga aggttacgtt ctgaaataga tagaatgttg caaaatattc tagcaatccc
     1501 taggatcatt cacatgacct tagggattgc ga
//
LOCUS       DAZKAS010000265         1509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000265 DAZKAS010000000
VERSION     DAZKAS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.273"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..868
                     /locus_tag="ACG8H7_002874"
     CDS             101..868
                     /locus_tag="ACG8H7_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP8984916.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ACG8H7_002875"
     CDS             1060..1458
                     /locus_tag="ACG8H7_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP8984917.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAZKAS010000266         1501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000266 DAZKAS010000000
VERSION     DAZKAS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.275"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1457
                     /locus_tag="ACG8H7_002876"
     CDS             <1..1457
                     /locus_tag="ACG8H7_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFP8984918.1"
                     /translation="QDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANG
                     GLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALP
                     FGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAP
                     LIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFI
                     RNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEK
                     PRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIF
                     ILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLG
                     SFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGT
                     ESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          408 a          288 c          317 g          488 t
ORIGIN      
        1 agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga
       61 tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat atgtacgata
      121 cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct
      181 acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag
      241 gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg
      301 ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg
      361 cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc
      421 gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac
      481 cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg
      541 ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg
      601 gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta
      661 acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc
      721 atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt
      781 attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat
      841 gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt
      901 catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga
      961 tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat
     1021 tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc
     1081 ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg
     1141 gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg
     1201 ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca ccacgagcat
     1261 ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg
     1321 cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg
     1381 cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa
     1441 tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc
     1501 t
//
LOCUS       DAZKAS010000267         1476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000267 DAZKAS010000000
VERSION     DAZKAS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.276"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8H7_002877"
     CDS             131..505
                     /locus_tag="ACG8H7_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFP8984919.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8H7_002878"
     CDS             complement(619..1248)
                     /locus_tag="ACG8H7_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP8984920.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8H7_002879"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8H7_002879"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          499 a          225 c          258 g          494 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataata
//
LOCUS       DAZKAS010000268         1451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000268 DAZKAS010000000
VERSION     DAZKAS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.277"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACG8H7_002880"
     CDS             complement(<1..625)
                     /locus_tag="ACG8H7_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFP8984921.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACG8H7_002881"
     CDS             complement(784..1050)
                     /locus_tag="ACG8H7_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8984922.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACG8H7_002882"
     CDS             complement(1040..1396)
                     /locus_tag="ACG8H7_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP8984923.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAZKAS010000269         1444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000269 DAZKAS010000000
VERSION     DAZKAS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.278"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8H7_002883"
     CDS             complement(150..1337)
                     /locus_tag="ACG8H7_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFP8984924.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          422 a          312 c          252 g          458 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taat
//
LOCUS       DAZKAS010000270         1443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000270 DAZKAS010000000
VERSION     DAZKAS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.279"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..804
                     /locus_tag="ACG8H7_002884"
     CDS             43..804
                     /locus_tag="ACG8H7_002884"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8984925.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(919..1359)
                     /gene="fabZ"
                     /locus_tag="ACG8H7_002885"
     CDS             complement(919..1359)
                     /gene="fabZ"
                     /locus_tag="ACG8H7_002885"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP8984926.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          485 a          276 c          265 g          417 t
ORIGIN      
        1 atcattaaag ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt
       61 attttaagta ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac
      121 acgacttctt ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg
      181 actgccagta gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc
      241 gaaaatgaag atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt
      301 cccgaacacg caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac
      361 aacggactga ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa
      421 ggcgatggca ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac
      481 cacctttata atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac
      541 ggcagccagt tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac
      601 gactatcctc aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc
      661 cattatactg tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat
      721 atcgaaacag atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata
      781 ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt
      841 atgctaggat tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg
      901 ctatatcagg ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca
      961 gactttttta tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt
     1021 ggtgatttca aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc
     1081 cttattgatc ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag
     1141 tatcgaacct gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc
     1201 aggaaagtga ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt
     1261 ttttcctggt tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat
     1321 caattccatt acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg
     1381 caattgccat tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc
     1441 ttt
//
LOCUS       DAZKAS010000271         1440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000271 DAZKAS010000000
VERSION     DAZKAS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.280"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACG8H7_002886"
     CDS             105..1406
                     /locus_tag="ACG8H7_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8984927.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAZKAS010000272         1426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000272 DAZKAS010000000
VERSION     DAZKAS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.281"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..191)
                     /locus_tag="ACG8H7_002887"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="ACG8H7_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            227..826
                     /locus_tag="ACG8H7_002888"
     CDS             227..826
                     /locus_tag="ACG8H7_002888"
                     /inference="COORDINATES: protein motif:HMM:NF023433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984928.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIERT"
     gene            855..1115
                     /locus_tag="ACG8H7_002889"
     CDS             855..1115
                     /locus_tag="ACG8H7_002889"
                     /inference="COORDINATES: protein motif:HMM:NF023433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984929.1"
                     /translation="MDYQLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDL
                     HRTFAQLPVELQPYAEMILNDVNNGELVIKEGWEFTDYLNEF"
BASE COUNT          518 a          218 c          261 g          429 t
ORIGIN      
        1 gttttaagca actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa
       61 cgggcctttc taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca
      121 agggtcgtaa atagctgatt ggttgtccca tcaacacaaa taaaactcat ggaaccagaa
      181 gtggcaacat tgtgtacttt tctttgcaat cttgtgtaca aaactattgc aaaacacagc
      241 gcataagaat ccttacaaaa cagtagatga agcaacccaa ttagatcttg ttatagttgt
      301 taatcagctc ttaacgggtt acgattcaaa atgggtcaac accctatact tagataagtc
      361 acttgaatat cagaacataa ttcaggcttt ttcacgcaca aatcgtctta atggtcctga
      421 aaaaccgttc ggaaccattc gttattatcg taaagcacat acgatggaac agaacattga
      481 agaggctatt gagttatact caggcaaaca agcaaaaggg ttatttgttg aaaaattata
      541 taataactta gaaaagttga acgatatatt cgaagaaatt aagtccgtat ttaatatttc
      601 tggcatagaa aacttcagtc atttaccgga ggttccaggg gaaattaagc aatttgtaaa
      661 tctttataat caactaatcc attgttatga atcagcgatg atacagggat tcacatgggc
      721 aaaatcgact tatgaaatcc aggattatga acctataaca gtagtaatca cagaatatga
      781 atttaatatc ttggcacaac gttacagcga gatagagaga acctagagac ggaaatgata
      841 gtatagcgta tgacattgat taccaattac ctgaaggtag tcgtagtcgt attaattatg
      901 agtatttaaa cacaaacttc aaaaaataca tgaatataaa agaagaacgt gattcgaatt
      961 taattcaatc attgttaaac gacttacatc gcacatttgc gcaattacca gtggagttac
     1021 agccatatgc tgagatgatt ctaaatgatg taaataatgg ggaattagtt atcaaagaag
     1081 gatgggaatt tactgattac ctcaatgaat tttagtggat tcaaccaata ttaatgaggc
     1141 attattacgt gagatgttgg atttacgctt aaccgaggta aacattaatg aatatagtcg
     1201 atttgataaa ttaaaatcaa gtgttaacgt aggtcatttc agtggatata tcgagaaaaa
     1261 tttaggtaaa atggtaatac caatcaaggt aaatatgcta attgacagat tattgcgtgc
     1321 atttttatta gaagatgtat tcgatgttac agagtacata aagaattact ttaactagag
     1381 tacacctaca tcttatagta cataaaaaaa ttcatctttt ttagtt
//
LOCUS       DAZKAS010000273         1424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000273 DAZKAS010000000
VERSION     DAZKAS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.282"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..276)
                     /locus_tag="ACG8H7_002890"
     CDS             complement(<1..276)
                     /locus_tag="ACG8H7_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFP8984930.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSI"
     gene            401..1186
                     /locus_tag="ACG8H7_002891"
     CDS             401..1186
                     /locus_tag="ACG8H7_002891"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFP8984931.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1179..1421
                     /locus_tag="ACG8H7_002892"
     CDS             1179..1421
                     /locus_tag="ACG8H7_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984932.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          533 a          232 c          218 g          441 t
ORIGIN      
        1 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
      361 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
      421 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
      481 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
      541 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
      601 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
      661 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
      721 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
      781 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
      841 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
      901 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
      961 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     1021 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     1081 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     1141 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     1201 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     1261 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     1321 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     1381 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DAZKAS010000274         1423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000274 DAZKAS010000000
VERSION     DAZKAS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.283"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          536 a          162 c          292 g          433 t
ORIGIN      
        1 ttaatttgac aattaagaaa gtattaactt gttttgtgaa aaagagaatt tgaaaaacga
       61 ttggcgaaca aatgctcggt aggtatggta taaatacaga gctaattggt gtgatgaaat
      121 cagatattcg cgtgagattc agtaaagtag gagttcgaaa acagctaggt gacaaacagt
      181 attgctttgg tacttcggaa tggtttatca aattcttatc aacagttcac ttctctttat
      241 gtttgagtga tgattcgtta ggttattgct tggaaagacc ggatgagagt agaagagttt
      301 aattttataa agtgaaaaga ggtaatgtac gatatacgta gggctgaacc aatagaggat
      361 aagcaaacga tgttacaggc gcaaaattca tagcttactg ttttggagat aacagaagag
      421 gaaataatgc agaagtaagg cgaaaaaagg acggaagcct aacagcatat gaaataaaga
      481 aaaatattat aactactgat aaatcggtat tgagcaaagt actaatagga gtcaaaaaca
      541 ttcataagct atgagtgttt cttggctact tttttatttt tatgtatcta caaaagtttc
      601 caggggcaga acctgtgatt attaatggaa gaacctcaag tattgtctta aatcattgtt
      661 atgaaaatgg attaaaagag ttcatcaagg tgtgattaac aagttacttt tcattacatc
      721 gaatttttca gaagttgctt atattggtga tgatattaat gatttatcat gtatgacatc
      781 tgtgaagaag cgggaggaat agtagaatgt ccaaaaaatg ccacaagaaa tgagattgaa
      841 ttatctgatt ttattgtaac tcacagtggt ggtgacgaat ctgtgataga gtttattgag
      901 tggatattgg aatcgatata gtgtagtagt acagaaaaat tatcatacaa ttaatactag
      961 gcataagaat attggcatat tctagagtta aaagtaacta ttcgaaaagg ataaggttag
     1021 aattttttgt tttgccagtg gaagaatata aaaagggcat tggaaattaa tatattagaa
     1081 tgctaagcgg agaaaaaaat atatgaaaag tacgtccttg tgatgggatg gaaaaataca
     1141 cttatttcaa acaacgataa tataattatg tgaaccgaag tatactcgct tctaattcaa
     1201 tcctcaaatc ccgacagtcg cgacttcaaa aaagcgtata gttttaatgt atccccatat
     1261 gatccgaaaa atggtaattt acaattaaca gtttgatgat gttgaagatg tggaaatgct
     1321 gaaattacca tgatgagaaa cacattaatt aaagggaatt aattaactag aatataatct
     1381 gattcaattt gaattaagca aaaaaaggct gagttttaaa taa
//
LOCUS       DAZKAS010000275         1371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000275 DAZKAS010000000
VERSION     DAZKAS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.285"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..285)
                     /locus_tag="ACG8H7_002893"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACG8H7_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACG8H7_002894"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACG8H7_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          489 a          221 c          183 g          478 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat a
//
LOCUS       DAZKAS010000276         1355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000276 DAZKAS010000000
VERSION     DAZKAS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.286"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG8H7_002895"
     rRNA            <1..1355
                     /locus_tag="ACG8H7_002895"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZKAS010000277         1343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000277 DAZKAS010000000
VERSION     DAZKAS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.288"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..347)
                     /locus_tag="ACG8H7_002896"
     CDS             complement(<1..347)
                     /locus_tag="ACG8H7_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP8984933.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAIL"
     gene            635..1288
                     /locus_tag="ACG8H7_002897"
     CDS             635..1288
                     /locus_tag="ACG8H7_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP8984934.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          500 a          168 c          242 g          433 t
ORIGIN      
        1 atgagaatcg cctgcttcag ttcctttgtt agttgatgat gttcatcaac taagtccgtt
       61 ttcgcagaaa aggtctgccc acaatcatag catagaaaac gggtacgttt taattctaaa
      121 atagtcaact tttctttgac tttcaatatc tgagtacgtg tcgtatagga accgttgcgg
      181 ataatacgaa tcgatccaca gtgttcacaa tgggaaggtg agtcatccaa tctcccttta
      241 ataaactgcg cttgaacgcc tttatattct ttttcaatca gccattcttc atcaaaaaat
      301 aaatgtttat ctgttaatcc gagtaatttt ctagtatgat tatccataga gcgactcctt
      361 tggtagggtg atgttttggt cgactaaatt ttaccaaaag tgagtctctt tttgagttga
      421 aacgaagaaa aagtgttagt agaaaatcgc cttcgatttt ccaacaacac aaaatattat
      481 agaacccttt attctaaagg atttcaaggt gtctgtttgc atttattaga aaaaagaaaa
      541 agtattggca acagaaggtg tcaccaacac tagatattat agagccataa tatgctgtat
      601 aatatctagt gtataggctt aagtggaggt atatatgaaa atattaactg ttgaagatga
      661 taatatgata agagagggaa taagtgaata tctttcagaa tttggatata ctgttattca
      721 agctaaagat ggaagagaag ccttgtcaaa atttaatagc gatataaatt tggttatctt
      781 agacattcag atacctttta taaatggttt agaagtgttg aaagaaatta gaaagaaaag
      841 caatttacca attctaatct tgactgcatt tagtgatgaa gaatataaaa ttgatgcatt
      901 tactaattta gtagacggat atgtagaaaa gccattttca ttgccggtct taaaagctag
      961 aatagattct ttaattaaga agaattatgg acatttagag aagtttgaat ataaggatct
     1021 atcggttaat tttaatagct atacagctaa aataaatggt gaagaaatag atgtaaatgc
     1081 aaaagaacta gaagtattaa aatgtttatt ggataataat ggacaagtgt taacaagaat
     1141 gcaaattatt gattatgtat ggaaagatag cgaagaaact ccttatgatc gagtaataga
     1201 tgtatatata aaagaactta gaaaaaaatt acaattagac tgtataacta ctataagaaa
     1261 tgtaggatat aaattggaga gaaaatgaaa aaattaaaga tatttccaaa aatgttcata
     1321 caaatatttt ctgttcttgg aat
//
LOCUS       DAZKAS010000278         1336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000278 DAZKAS010000000
VERSION     DAZKAS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.289"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..>1336
                     /locus_tag="ACG8H7_002898"
     CDS             56..>1336
                     /locus_tag="ACG8H7_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFP8984935.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          453 a          260 c          254 g          369 t
ORIGIN      
        1 tctctagtat gattaaatca ccacaaaata aacaactgag aggacgatac gctatatgaa
       61 tgattctatc aaaaaaatgc tgagaataac agagaaagat ttgatgatta cagaggtctc
      121 ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat gctgttctct cgcctactcc
      181 tcgtgcttgt agaagttgtg gttctactgt ggtagatgga aacgggaaag caattatagt
      241 gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt
      301 tatgcgccta aaaaagcaac gctatacctg taaaaactgt cgcacccatt ggacggctca
      361 aagttatttt gtccaaccca gacattcaat cgcaaatcat gttagatata aaattgcttc
      421 tttactgact gaaaaagtat ctttatcttt tattgcgaaa agctgtcagg tatctttgac
      481 caccgttatt cgtacattga aagagtttaa aagctattta ccaaagcaat ctaagaagat
      541 cctccccaga gtattgatgg ttgacgaatt tcgttcgcat gcttccatag aagataagat
      601 gagctttatt tgcgcggatg gcgaaacagg aaaattaata gatgttttgc ctacgcgtaa
      661 attacctcga ttaacaagct atttcttagg ttgtaccaat ccagaagaag tagaattctt
      721 ggtgacagac atgaacgccg cctacttcca gctcaccaaa cgtgttctgc caaatgcgaa
      781 agtcgtgatt gatcggtttc atattgtcaa acacatgaat caagcgttca atgagttgcg
      841 tatccgtgaa atgaatgaac ttcgtaaagc cggacagaaa agccaggcag aaaaactgaa
      901 aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc aaccattatg aatacaaaac
      961 atggaaaagt ttccgagcac caaaataccc atttcttact gaagcaatga tgattgatcg
     1021 attgcttgag ttttctccgc ccctaaagga ggcgtatccc ttttttcatg aattagttga
     1081 agcctttcga gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac
     1141 gttagatgac agctttcggg aaaagcttca aaaccttctg acctatgaag aaggcatcac
     1201 caacgcaatg atctatcctt attccaatgg aaaaatagaa gcgaagaata cccacataaa
     1261 gacaatgaaa cgagtatcct acggatttaa atcatttgag aacatgagaa ttagaatctt
     1321 tttgatcaat caatta
//
LOCUS       DAZKAS010000279         1319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000279 DAZKAS010000000
VERSION     DAZKAS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.290"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            329..1240
                     /locus_tag="ACG8H7_002899"
     CDS             329..1240
                     /locus_tag="ACG8H7_002899"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP8984936.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          522 a          200 c          256 g          341 t
ORIGIN      
        1 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
       61 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
      121 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
      181 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
      241 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
      301 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
      361 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
      421 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
      481 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
      541 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
      601 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
      661 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
      721 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
      781 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
      841 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
      901 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
      961 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     1021 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     1081 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     1141 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     1201 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat
     1261 gaactattta aatcaataaa actgttcatc taaatctttt tattagaact aaatcaaca
//
LOCUS       DAZKAS010000280         1314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000280 DAZKAS010000000
VERSION     DAZKAS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.291"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG8H7_002900"
     CDS             <1..963
                     /locus_tag="ACG8H7_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFP8984937.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACG8H7_002901"
     tRNA            1152..1225
                     /locus_tag="ACG8H7_002901"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          435 a          253 c          263 g          363 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgag
//
LOCUS       DAZKAS010000281         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000281 DAZKAS010000000
VERSION     DAZKAS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.292"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8H7_002902"
     CDS             <1..1244
                     /locus_tag="ACG8H7_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP8984938.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKAS010000282         1286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000282 DAZKAS010000000
VERSION     DAZKAS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.293"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..437)
                     /locus_tag="ACG8H7_002903"
                     /pseudo
     CDS             complement(117..437)
                     /locus_tag="ACG8H7_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(553..>1286)
                     /locus_tag="ACG8H7_002904"
     CDS             complement(553..>1286)
                     /locus_tag="ACG8H7_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984939.1"
                     /translation="LCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLKNGKSDLTHS
                     IKKMNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPHEDYKIIMNY
                     YLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLID
                     LKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDL
                     LIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT          407 a          217 c          173 g          489 t
ORIGIN      
        1 gttttagaaa aagcaaaaaa ataaagaaac ttattcttga taacaagcta cgtgacgtat
       61 atccctaaca caaaaatagc catatccttt ggtttggata cggctatttg atattactca
      121 ttcaattatc aaatcacagt aggtgtgaat ctcatcggga tcggcaatat ctacttctga
      181 ttaggatcaa aggtgatttt ctcaataaag gtgtgataca aagctttaac ttgcttcttc
      241 tcactctgga taagaaagca atccaaacta gtcaatatct gttggacatc tttgacttga
      301 atcggttccc cttgttgttg aaagatccct aagatttcat tctctcgaat ttgtaactgt
      361 cttcgttttt cagtcaattc atttaaacga tcttttaaca tcggaaacaa ttcgggacta
      421 tcttccaaag cagcttcctg tttttgctct aatagttaga ttttgcgtac cattttcttc
      481 ttttattttc cttcgattgc tgattttaat atagtctaaa ggaaatttat atttattgta
      541 ttctttataa cttcaagaga acttcgtttg agtattactg tattttttta attccataag
      601 aaaattagtt attacctgcc acctaatcag caaatctgaa cctgagtcaa tataattaat
      661 attattttcc atctccaaca ttgtaagtac tgttggtgtt aatataggga ctttgtacaa
      721 ttgtattttt ttgattttat catctgcaca aaggagattt tttaaatcta atccttcttt
      781 tatttcaaaa gttgaatttt ttaaatctat aagagtaaaa ccaattcctt catactgaat
      841 ttctgaaaat cgaatttttc tcccagttga tagatcaagg ggcagaaaac cttttattac
      901 attgtgagtg tcaccatcat ttacaatgtc attaggaatt ttgattttag aattctgtgg
      961 agcacctatt ggaagataat aattcattat aattttgtag tcttcatgag gaaataaagt
     1021 acttttagga ttgctaagat attctcgtac cgaatctaag aatggcttat ccattgaatc
     1081 ttcatcccca tttaaactaa aaaacattcc tacaattgct tttgcaactc taagtgaatt
     1141 cattttcttt attgaatgtg ttaaatcaga cttaccattc tttaaacttg tattgttata
     1201 aaggaaattt ttattgtctt ctgcaaatct aatatattcc tcaacatact cactaccaaa
     1261 aaaactatta catttactac ataacg
//
LOCUS       DAZKAS010000283         1280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000283 DAZKAS010000000
VERSION     DAZKAS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.294"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1024
                     /gene="scrK"
                     /locus_tag="ACG8H7_002905"
     CDS             152..1024
                     /gene="scrK"
                     /locus_tag="ACG8H7_002905"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFP8984940.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          402 a          230 c          287 g          361 t
ORIGIN      
        1 aaaattatta gaattttaaa aaatgataaa aattcaaaaa gaaaaaatag tagttattta
       61 cttgtcatgt tactaaataa aaaaataatt taaatatgat atatagtcat ttatttagtg
      121 atttcattct aaaataaagg gagaaatgtc tatgctatac ggtgcaattg aagcaggcgg
      181 cacaaaattt gtgtgtgcca ttggcgatga agaaatgacg atcaaggaaa gagtgagctt
      241 tccgacaacg acacctgagg aaacaatgcc tttagtcatt gacttcttca aacaatacca
      301 agcagattta gcagggattg ggattggctc atttgggcct atcgatatcc atcgcgattc
      361 cgccacttat gggtacatta cctcgacccc gaaattggca tggcaaaatt ttgatttcat
      421 cggtaccatg aaaaaagaat ttccgatccc gatttcttgg acaacagatg tcaatgcggc
      481 tgcctatggt gagtatgtct tcggcagtgg caaaggattg agcagtgtcg tttactatac
      541 gatcggtacg ggtgttggcg gcggtgcctt acaagaaggg cgctttatcg aagggtttag
      601 tcatccagaa atggggcata tgctcgtcgt ccctcatcca aaagatgact ttgctggcag
      661 ctgtccattt catggcaact gtttagaagg gatggccgct ggacccgcca tcgaaaaacg
      721 cttagggaaa aaaggacaag aggttgcaga agatgatcct tattgggaaa tcgaagcctc
      781 ctatattgct caatgtgcct acaatacaac cttgatgttt tctccagatg tgatcatttt
      841 tggcggtggt gtgatgaaac aacgccatat gctgcaaaac gtccacgacg catttgcaaa
      901 attggtcaat gggtatgtcg aaacacctga gttaagcaaa tatatcgtga ccccagcatt
      961 agaagacaac gctggaacgc tcggttgttt ggcattggct cgagaagcgg tcttacattc
     1021 ataaacgaaa aaaggctctt cgacatttag tgttggtaga gaaagataca actgaaaatt
     1081 tagtcataac cagttaggca gagtgcttaa ctggtttttg ttttacttgg aagatcaagc
     1141 cttgaatgag ataaattctt gctcgaaagt tatggaaatt tcgatagccg taggccactt
     1201 gtattgtgca atagtttttt acacgccgtt tgtataatta agcgagacaa ataaaaaagc
     1261 catttatgtt acactcaaat
//
LOCUS       DAZKAS010000284         1272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000284 DAZKAS010000000
VERSION     DAZKAS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.295"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1170)
                     /locus_tag="ACG8H7_002906"
     CDS             complement(76..1170)
                     /locus_tag="ACG8H7_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFP8984941.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          375 a          270 c          197 g          430 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tg
//
LOCUS       DAZKAS010000285         1258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000285 DAZKAS010000000
VERSION     DAZKAS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.296"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1223
                     /locus_tag="ACG8H7_002907"
     CDS             <1..1223
                     /locus_tag="ACG8H7_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP8984942.1"
                     /translation="EFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSL
                     NVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQF
                     LGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSI
                     TEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELV
                     IIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHY
                     LEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSN
                     NQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEI
                     PDLELRLLSDIVQG"
BASE COUNT          444 a          163 c          241 g          410 t
ORIGIN      
        1 acgaattttt tgctaatgtt cttgaaaccc cttattggta tgcaaaaaaa atcttaagcc
       61 ttttatctga gtgtttagat caacctgtgt atgaagagga cattttcttt attacgctat
      121 atttttatag tttgaatgtc acgaatgtta aaacaggtat taagagtatt gtgctagctc
      181 atggttattc tacagctagt agtatggcga atgttgccaa ccgtatgctg aataagaacg
      241 tttttcaagc ttatgatatg cctttagata ttacattaga gaaaattgaa gcaaaattga
      301 ttcaattcct tggagattat cgaacagatt caggcttgat tttattggta gatatgggtt
      361 cactaaacca actaggcggt ttattagtga atcatctaaa tggaccactg ttaatcattg
      421 atcatatcaa tacaccgtta ttattggaag tcggccaaca agtattaaac aataaaagta
      481 tcaccgaaat aggagcagaa attgaaacaa gtcttacttt caaaaagcag ttactacttc
      541 ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta tacagggatt ggtagtgcga
      601 cccagattca ggagatttta acgaaatgct tgaaagatgc agttagagaa ttagtaatta
      661 ttccgtatga ttacaaaaag ctgttagaaa acaagaattt tgagactcct tttcaattat
      721 acgatgtcat catgattgta ggtacagaag atcccaaaat taatcagatt ccatatattg
      781 gattagatcg cctgattaat ggggaagcaa ttggcgaatt tgttcattac ttggaaaaag
      841 agctgactat tgaagtgaac gatttacaaa aagaattaat tttcaatttt tcaattagta
      901 aaattgtcga aaatttaacc attttggatc cagataaggt gctatctttt gtacaaaaag
      961 caattaaaga attatcgaag ttattaaatt ttgatttttc taataatcag ctgtttttgt
     1021 tgtacattca ctgtagttgt atgattgaac gaattgtacg taaagaaagt gtggatgagc
     1081 aagaggacat tgaagaatat atgcaagcaa atggagaaat aatggataaa attcatagcg
     1141 ctttttttga gattgaaagg gaatacacaa ttgaaatacc agatttagag ttgcgattat
     1201 tgagcgatat tgttcaagga taggagtgaa ttcgaatgac aatgggagta atttttga
//
LOCUS       DAZKAS010000286         1247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000286 DAZKAS010000000
VERSION     DAZKAS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.297"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..207
                     /locus_tag="ACG8H7_002908"
     CDS             <1..207
                     /locus_tag="ACG8H7_002908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984943.1"
                     /translation="IRETNNTFLCVAYFPLTIVFYLMVNLLKINTATTNGDQACFVTY
                     FTIITTLFYYSILLPYPSIPNHLW"
     gene            complement(249..1109)
                     /locus_tag="ACG8H7_002909"
     CDS             complement(249..1109)
                     /locus_tag="ACG8H7_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP8984944.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          377 a          237 c          182 g          451 t
ORIGIN      
        1 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
       61 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
      121 tttgtaacgt attttacgat aattacgact ttattttatt actctatctt gctgccatac
      181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
      241 ttttttcctt agttaatcaa tagtatcatt tttttcatat gatttaattc atcaattaat
      301 tttttgtttt tattaatttt agcaaaagct agtgcatctt gaatcaattc gagcacttct
      361 gccttcgact tatttttagc ctgtgcgttc ttcgctaatt gggcagcagc tcgtgctaag
      421 taataagtca aatggttatc cgaacaaata tcgattgcgt aatttagtag tgcatcactt
      481 gtttccaaat caccgtcttg cgcaaaaaaa acaccacaat gaaagacgat atttaacacc
      541 cgccagatat cttctgttgt acgaatcgga tacgtataaa ttttttcaaa cactcgattg
      601 aaataaactt ctgctttttc caactctttt tctctagcat agaccattcc aatacctgta
      661 tatgccaata aacgaaacaa cgtgttggct gtttctgcca tcaacaactg atcaaaatgg
      721 aacaacacat ccgtaattgg tgcattcgta aaaatcattt caaatccttt tagatagtag
      781 taacgtgcaa tcagctcaga ctcgtcaatt accttgggat caatggctgc taaaatctgt
      841 gtcaattgtt catattcact cgtaatcagt gaaaactctg ctttttctag ctgttcatca
      901 agttgtgtat tgatcacgtt catcgaagaa aaaagttcaa ctaacggaag attgattcga
      961 ttacataatt tgattaaaat ttttaacgaa gggacttgac cattgttctc aaatttactt
     1021 aatgttgctt gtgtacaaat tccttcagac acttcttttt gagataagtt ggcagccttt
     1081 cttgccgtaa taaatttttc aatgttcatt attttctctc caatcagacc tcacacgaaa
     1141 atcatttctg tctcgatttt agcatgtttt ctttgggaat gctttaggtt ctgttgcaaa
     1201 gttttaaata aagaataaag tcccttacgg tatctatgat ttaagct
//
LOCUS       DAZKAS010000287         1246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000287 DAZKAS010000000
VERSION     DAZKAS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.298"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8H7_002910"
     CDS             200..742
                     /locus_tag="ACG8H7_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984945.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG8H7_002911"
     CDS             898..>1246
                     /locus_tag="ACG8H7_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFP8984946.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZKAS010000288         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000288 DAZKAS010000000
VERSION     DAZKAS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.299"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8H7_002912"
     CDS             414..947
                     /locus_tag="ACG8H7_002912"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP8984947.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKAS010000289         1209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000289 DAZKAS010000000
VERSION     DAZKAS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.300"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1209
                     /locus_tag="ACG8H7_002913"
     CDS             <1..>1209
                     /locus_tag="ACG8H7_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP8984948.1"
                     /translation="FSSKILVFLLVPLYTSVLSTTEYGTYDLTISTATLLFPILTLNI
                     VDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLLNITKLWQDVYGLEKYIFL
                     YYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANI
                     LSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVV
                     SFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMIN
                     LLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKD
                     SKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKH
                     YHIVVESWGLL"
BASE COUNT          391 a          149 c          218 g          451 t
ORIGIN      
        1 ttttcttcaa aaattttagt ttttttgtta gtccctttat atacaagtgt attatccaca
       61 acagaatatg gcacatatga tttgacaatt tcaacagcca cattattgtt tccaatttta
      121 accttaaaca ttgtagatgc tgtgatgcgc tttttaatgg ataaagataa aaataaaaaa
      181 aatatagcat ctatagcgat taaagttata tttttaagcg tgatagtctt tggatccgta
      241 atgtatttgt taaatattac aaaattatgg caagatgttt atggattaga aaaatatatt
      301 tttttgtatt atatatccta tgtatggaat caatttctaa tacaatttgc taaagggtta
      361 gaaaaagtta aagctatggg catagcaggt attatcagca caattgtaac aataattgcc
      421 aatattttct tcttgcttat tctgaaatgg ggattaattg gttttttcat tgcaaatatt
      481 ttatcacaga ttatttcagc tacttatctg tcagtattag tgagaatatg ggaatatatc
      541 gattttgagg ctaaagatcc tcagctaaag agagaaatgc taaattactc gattccatta
      601 attgcaactg ttgttggatg gtggattaat agtacagctg ataggtatgt ggtttccttt
      661 atagtaggtg tctcggcgaa tggcttattg tctgtggcat ataaaatacc tcaaataatt
      721 aatactttgc agggaatatt tatacaggcc tggcaaattt ctgcaattaa agaatatgga
      781 gataatgaga ctgcaaaatt ttatggaaat acatttgtta tgattaattt gttaatgtgc
      841 ataacctgtt caagtcttat catattaact agaccgtttt cacatattct ttatgcgaaa
      901 gacttttatg aagcgtggaa atatgttcca tttttattaa tagctaatgt tttaaattgt
      961 gcatcaggtc tgttaggacc tatcttatca gcaaaaaaag attctaaatc catgatgtgg
     1021 tctgcaatca taggggcatt agttaatgtg attatgagtg tagtccttgt ttataagata
     1081 ggggttcaag gtgccacaat agccactgtt atatgttcat atgtaatata tgttgttcgt
     1141 aaacgagctg tgggaaaaga tatttttata aagcattatc atattgttgt ggaatcttgg
     1201 ggattattg
//
LOCUS       DAZKAS010000290         1203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000290 DAZKAS010000000
VERSION     DAZKAS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.301"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1203
                     /locus_tag="ACG8H7_002914"
     CDS             <1..>1203
                     /locus_tag="ACG8H7_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFP8984949.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQL"
BASE COUNT          399 a          234 c          232 g          338 t
ORIGIN      
        1 cagaagaaaa agacgttgat cgtcgatgct gttttctcac ctgctcccca tacctgtaga
       61 aattgtggtt ctactgtggt agatggaaac gggaaagtaa ttgtagtgaa aaatgggaag
      121 aaggaaacga ttgtccgctt tgaacaatac aatcacatgc ctttggttat gcgcttaaaa
      181 aagcagcgct atacctgtaa aaactgtcga acccattgga cggctcaaag ttattttgtt
      241 caacccagac attcaatcgc gaatcatgtc agatataaaa ttgcttcttt actgactgaa
      301 aaagtatctt tatcttttat tgcgaaaaat tgtcaggtat ctttgaccac cgttattcgt
      361 acgttgaaag agtttaaaag ctacttacca aagcaatcta agaggattct ccccagagta
      421 ttgatggttg atgaatttcg ttcgcacgct tccatagaag ataaaatgag ctttatttgc
      481 gcagatggcg aaacaggaca attaattgat gtcttgccta cgcgtaaatt gcctcgatta
      541 acaagctatt tcttaggctg cgctaatcct gaagaagtaa aatttttagt cacagacatg
      601 aacgccgcct acttccagct taccaaacgt attctgccaa atgcgaaagt agtgattgat
      661 cggtttcata ttatcaaaca catgaatcaa gcctttaatg agttgcgtat ccgtgaaatg
      721 aatgaacttc gtaaagtagg acagaaaagt caggcagaaa aactgaaaaa gaactggcgc
      781 tttttgctaa aaaatcgtgc aaacatcaac cattatgaat acaaaacatg gaaaagtttt
      841 cgagcaccaa aatacccatt tcttacggaa gcgatgatga ttgatcgatt gcttggcttt
      901 tctacgtccc tgaaggaggc gtatccctat tttcatgaat tagttgaagc ctttcgagac
      961 aaagaccctg acttattttt ctccttattg gcagaacttc ccgaaacgtt agatgacggc
     1021 tttcgggaaa agctccaaaa ccttctgacc tatgaagaag gcatcaccaa cgcgatgatc
     1081 tatccttaca ccaatggaaa aatagaagcg aagaataccc atataaagac aatgaaacga
     1141 gtatcctacg gatttaaatc atttgagaac atgagaatta gaatcttttt gatcaatcaa
     1201 tta
//
LOCUS       DAZKAS010000291         1194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000291 DAZKAS010000000
VERSION     DAZKAS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.302"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..1188
                     /locus_tag="ACG8H7_002915"
     CDS             73..1188
                     /locus_tag="ACG8H7_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984950.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
BASE COUNT          451 a          177 c          217 g          349 t
ORIGIN      
        1 agaaaatctt agtcaaaaaa ttatataagt ttgcgtacga ttcaaagaaa attaagagaa
       61 cttaatacta ttttgaaaaa ctatcattta tcttgcagct tgaaaaaagg taatccgata
      121 gaaggagaag aataccgtat ccgatacttt tttcacttga tgtattggca gtcattcgac
      181 gaaaatcatt ctgatatatt atcaagtata aaaaataaac gtcaactatt agaaaaattt
      241 gcgaaacatg cgccatatac gcgtagtata gatagaaaga aatggatcag cctactaaga
      301 atttcgttgc aacgaatgaa acaaggccac tcgattacgt atgtcccaga agagatgaac
      361 aaatttgtgc atccattgat aacaaaagaa gagtttacgc attacatact agagcctttt
      421 tttgaagcaa acttctggtc gatgaatcac atcactccaa cagagcaagc ttacctttat
      481 tttatgtttg gtgtaatgaa cacctatttg gaagaagatt tgttggatac tccagataaa
      541 gtacaagaaa gttggggctg gattgaatcg tctattgtag aaaattttga aaaaaacttc
      601 aagatttgtt ttacaacttc agaaaaaaat tatctatatg tcaatttggc aatgatccat
      661 ttgtattcaa gggtttttgg gacaaaaaag aaggtggatg cttttggaaa atcagcagaa
      721 gacagagatt ttcagcatat atttccagtg atgtatccaa tcatgcaaca gttttttagt
      781 ggggtagctc gcagaattcc agagcttaat acttattatg aaaagaacag acggttgatt
      841 tttcaatact gtatgttagt cagggatatt tttataaaac acgaacaacc tgtcatattc
      901 tatattcaat caaagtatgg taaaacacag gaattatggg caaagaaaag gatattatct
      961 atgactgaac gcccaataaa atatagtgca tccgctgatc agttacctga tattgtcatt
     1021 agcgattatc ctatcgacaa aaaggcatat gatcaaacga atacacagat attttactgg
     1081 aatggacaac ctacaaaaaa tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg
     1141 aatgagaaaa atagatccat attggaaaaa ttggatttcg ttaattaaga aaag
//
LOCUS       DAZKAS010000292         1165 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000292 DAZKAS010000000
VERSION     DAZKAS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.304"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..171)
                     /locus_tag="ACG8H7_002916"
     tRNA            complement(82..171)
                     /locus_tag="ACG8H7_002916"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Ser,seq:tga)
     gene            complement(195..268)
                     /locus_tag="ACG8H7_002917"
     tRNA            complement(195..268)
                     /locus_tag="ACG8H7_002917"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Ile,seq:cat)
     gene            complement(282..355)
                     /locus_tag="ACG8H7_002918"
     tRNA            complement(282..355)
                     /locus_tag="ACG8H7_002918"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Met,seq:cat)
     gene            complement(383..455)
                     /locus_tag="ACG8H7_002919"
     tRNA            complement(383..455)
                     /locus_tag="ACG8H7_002919"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(420..422),aa:Ala,seq:tgc)
     gene            complement(475..548)
                     /locus_tag="ACG8H7_002920"
     tRNA            complement(475..548)
                     /locus_tag="ACG8H7_002920"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Pro,seq:tgg)
     gene            complement(558..631)
                     /locus_tag="ACG8H7_002921"
     tRNA            complement(558..631)
                     /locus_tag="ACG8H7_002921"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(595..597),aa:Arg,seq:acg)
     gene            complement(642..727)
                     /locus_tag="ACG8H7_002922"
     tRNA            complement(642..727)
                     /locus_tag="ACG8H7_002922"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Leu,seq:taa)
     gene            complement(744..815)
                     /locus_tag="ACG8H7_002923"
     tRNA            complement(744..815)
                     /locus_tag="ACG8H7_002923"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(781..783),aa:Gly,seq:gcc)
     gene            complement(827..899)
                     /locus_tag="ACG8H7_002924"
     tRNA            complement(827..899)
                     /locus_tag="ACG8H7_002924"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(864..866),aa:Thr,seq:tgt)
     gene            complement(917..998)
                     /locus_tag="ACG8H7_002925"
     tRNA            complement(917..998)
                     /locus_tag="ACG8H7_002925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Leu,seq:tag)
     gene            complement(1006..1078)
                     /locus_tag="ACG8H7_002926"
     tRNA            complement(1006..1078)
                     /locus_tag="ACG8H7_002926"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Lys,seq:ttt)
     gene            complement(1082..1154)
                     /locus_tag="ACG8H7_002927"
     tRNA            complement(1082..1154)
                     /locus_tag="ACG8H7_002927"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1119..1121),aa:Val,seq:tac)
BASE COUNT          303 a          331 c          278 g          253 t
ORIGIN      
        1 ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac cagactgctc
       61 catcccgcga taatatgaaa aaggaggata agggattcga acccttgcac ggttttaccc
      121 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc catatgaaac
      181 ttacaacgcc acaatggacc ttgtaggact cgaacctacg accggacggt tatgagccgt
      241 ctgctctaac caactgagct aaaggtccag gctcttaaaa atcgcggcgg aggggatcga
      301 acccccgacc tcccgggtat gaaccggacg ctctagccag ctgagctaca ccgcgaaaaa
      361 gatattcatt atttaaatga aatggagcct agcgggatcg aaccgctgac ctcctgcgtg
      421 caaagcaggc gctctcccag ctgagctaag gccccgtgtt taaatttata attatcggga
      481 agacaggatt cgaacctgcg accccttggt cccaaaccaa gtgctctacc aagctgagct
      541 acttcccgtt taattaacgc gcccaagagg agtcgaaccc ctaacctttt gatccgtagt
      601 caaacactct atccagttga gctatgggcg catatttgaa atgccgagga ccggaatcga
      661 accggtacgg tgatcactca ccgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      721 ccccggcgcg attgctttgg caaagcggaa aacggggttc gaacccgcga cccccacctt
      781 ggcaaggtgg tgctctacca ctgagctatt tccgcgtgtt taaagatgcc ggctaaagga
      841 cttgaaccct cgaccctctg attacaaatc agatgctcta ccaactgagc taagccggca
      901 tgtaaaactt aaattatgcg ggtgaaggga cttgaacccc cacgccgtaa ggcgctagat
      961 cctaaatcta gtgcgtctgc caattccgcc acacccgcaa aaatatgagc cgtacagggc
     1021 tcgaacctgt gaccctctga ttaaaagtca gatgctctac caactgagct aacggctcat
     1081 atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc
     1141 tgagctaaac ctccatggat aactt
//
LOCUS       DAZKAS010000293         1164 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000293 DAZKAS010000000
VERSION     DAZKAS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.305"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..>1164
                     /locus_tag="ACG8H7_002928"
     CDS             201..>1164
                     /locus_tag="ACG8H7_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984951.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIA"
BASE COUNT          385 a          118 c          202 g          459 t
ORIGIN      
        1 tatatacaat gagaagctga aatttacttt tatttgatga ctggaaatag aaattaaata
       61 tattgtattg ttacattaaa gaaaacttgt atggaaaaat cagtacaata ccattattta
      121 atgttcttca cttaaagcgc aaaaaatatc agtttattaa actcgtatat agaacaaata
      181 tatgtagacg gggcgaaacg atgagtgtaa aaaaaatgct atttatggta agtacaataa
      241 ttgtattaac atcccgattt tggggaatta atactagttt atctatgcga tattttatta
      301 tggcaatatg ggatttatat tttatgttaa gctatttttc ttatcgtgag aaaacatata
      361 aaaaatacgt tatcaagaat aacttttggt tgactataat gccgtttgtg atattttcta
      421 tctatactct aataatatgg ggaattggtt caaatgcaga atttgaaaat tttataaagt
      481 tatgttccac gttgctttat ttaattcttg catatggtta cgcatgtaca gcattttact
      541 tatttaaggc agaaggaatt gattttattt tttggtcagg tgttatttca tatatgagtg
      601 gttcagtttt ttatttaatt gtatcttatg gtttaaatgg tttaatttct tatataaaaa
      661 gtcttataac aggtgaaaca aatactgcaa attttgctat ggaggtacat gatttaactt
      721 ttgcgatggg ggtttttttc ctttattacg tattttttga aaataaaaat acaaaaaaac
      781 ataagataaa aattatcgta tctttaattt taattttcat gggattgaag agaattgaac
      841 tgttagcttt aagtggtgca attctagtgt attatgtttt attaaagtgg ggaaaaaaga
      901 ttcaaatccg tgcattagtg tgtactattt gtgcaactgt tatttcgtta gtgtttattt
      961 acattataga tacaggaatt ttggcattgc ttatgtcaaa tttgggcatt acagatgacg
     1021 gtgcgaggct ttcattttat ctatattcat ctaaatttta tgaacttggt ttaggatatg
     1081 taggaacagg atggacatgg ttttcaaaat acttttttaa tctttattta tcgcacttta
     1141 ggttaaatgg tggaagaatt gcag
//
LOCUS       DAZKAS010000294         1155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000294 DAZKAS010000000
VERSION     DAZKAS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.306"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..333
                     /locus_tag="ACG8H7_002929"
     CDS             58..333
                     /locus_tag="ACG8H7_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984952.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            760..>1155
                     /locus_tag="ACG8H7_002930"
     CDS             760..>1155
                     /locus_tag="ACG8H7_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP8984953.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTW"
BASE COUNT          436 a          201 c          173 g          345 t
ORIGIN      
        1 catataacca aaggcgctat gatttccata tgaaaaaaat aaagtcaggt gaaaaaaatg
       61 aaacttgagg ataaaaaagt cattttgaag aaaatcaaac gactaccaga ccaaatagat
      121 ggctatacga tctgttcttt caaaaaagac aggagtataa aagcttatac gcaaaacaca
      181 gaaaaaggaa taatctattt cctacatgaa gaaggttacg atacccaaac ctttatgttt
      241 actgaaaaaa aagaatttca taaacagatg aaaaaactga ttgaaaaaga atttccaaga
      301 agtcataaac tctatgtgaa tcagcaattt taacttctaa ctgttcttta ttaattaaaa
      361 atgttctgta ataaaataaa actttataac aactataaca aaaccgaatg caaaacagaa
      421 gttctataag ttataatcat agttgttagg tgattcatct agtaaaaaca ccagatttca
      481 aatttcacac aatttaaact agatcaatca tcaaccttct tcaaattgtt agaacccttt
      541 cccgaggagc tgcagcataa gcaaaaaaac aaacaggaag ccaaatggct aagacatttt
      601 ttctattagt ctagttgttt ctcgtcgcaa gtttaagact ttagaaagtt atttctaatg
      661 atgtttgggt tgttttgatc aatgcttatg cacagctcct tacctattat ttatctcaac
      721 agatctaaca aattaatgga ctatttaagg agttttttta tgcttacgat ctattcttct
      781 cgcacacata gttaccatct gatggcaaac tggctgaaag accatcaaat gccttacaga
      841 gaacgaattt tcacacctaa acagccttta acaaggaaag agatatttta tttattgtca
      901 ttaacagaaa acggatttga cgatctatta gctaaacgct cgattgatta taaaaatttg
      961 aaagaccaaa tcgaccaatg tacgacctca gagcttgtcg atctgatcgt ctcgcatttt
     1021 actttgttac aacgtcccat cattactgac ggaaagaaat tattagtcgg attcaatgaa
     1081 gatagcatgc gtctttttat ccctgtacaa tggcgcagaa gtcaactgaa atctgtctat
     1141 aacgatttta catgg
//
LOCUS       DAZKAS010000295         1116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000295 DAZKAS010000000
VERSION     DAZKAS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.307"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACG8H7_002931"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="ACG8H7_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFP8984954.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DAZKAS010000296         1106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000296 DAZKAS010000000
VERSION     DAZKAS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.308"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8H7_002932"
     CDS             <1..1087
                     /locus_tag="ACG8H7_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP8984955.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DAZKAS010000297         1090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000297 DAZKAS010000000
VERSION     DAZKAS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.309"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..199
                     /locus_tag="ACG8H7_002933"
     CDS             50..199
                     /locus_tag="ACG8H7_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984956.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            196..642
                     /locus_tag="ACG8H7_002934"
     CDS             196..642
                     /locus_tag="ACG8H7_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984957.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            644..781
                     /locus_tag="ACG8H7_002935"
     CDS             644..781
                     /locus_tag="ACG8H7_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP8984958.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            819..>1090
                     /locus_tag="ACG8H7_002936"
     CDS             819..>1090
                     /locus_tag="ACG8H7_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984959.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          405 a          172 c          222 g          291 t
ORIGIN      
        1 gattatacct ttgcaattac ttacacgatt tagaaaagcg tgaatcgata tgaaggcagc
       61 atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg aagaacattt
      121 accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga aattcaatta
      181 ctgggaggga aataaatgaa aaacatttgg aaatatggac gtactggcgg agagtacgca
      241 ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc accgcttgaa
      301 ggaattcgtg ctgatggcga accgttaaca atcgctgatc aaatgtttga tcctaaattg
      361 aatcaatgga ttattttagc gaacgcacta gatcacaacg atttaaacaa tctcaaagca
      421 atgtatgagt cgttagaaaa tgagaacggc gatttaaaac agatcaatgc caaactcatg
      481 ctaagcgatg tagcaattaa acaggaaaat actgcattga aagaaaaagc ggatagttta
      541 gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga catttcagaa
      601 attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta gttttagcga
      661 tgtgaaaatg atgtatgatt ggggctgttt cactaacgaa caagtaatgg ttttcgttcc
      721 gttgtgcatt actgaagaaa aagcagataa aatcattagc aaagaagaga gcgcatctta
      781 attgatgtgc tttttatttt gattcaagga gttgtcacat gattaattta ggggaatggg
      841 gagcgatagc aggatcaata accgctatcg tttctttgat tttattagta ataaaaccaa
      901 ttactgcatc tttctcgaag attactgaga ctctttcaaa agtaagtcac aatttagatt
      961 tgctgactaa agatttagaa tcgagcaaat cagatcgatt gatgattcat gaagaactaa
     1021 agaaacacga tgaaagatta gatacacatg cagaaaaatt ggtagaacac acgcaacaaa
     1081 ttaaaacttt
//
LOCUS       DAZKAS010000298         1089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000298 DAZKAS010000000
VERSION     DAZKAS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.310"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1089
                     /locus_tag="ACG8H7_002937"
     CDS             <1..>1089
                     /locus_tag="ACG8H7_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984960.1"
                     /translation="FSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINRALSLES
                     IFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFIC
                     HYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYD
                     SKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAI
                     VTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLT
                     LLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRFMI
                     FSTLFISFSVSLFISPQLEKFSYTRRRNF"
BASE COUNT          343 a          151 c          146 g          449 t
ORIGIN      
        1 ttttctgtaa aaatatttat agtttatgca tttgttagtt tattatattt atttccagca
       61 tttaaaaaca tggaaatatt tggcttttat gatacggcat ttcacattaa tcgtgcgtta
      121 agtttagaat ctatttttag ctcccctatt aattttgaaa cgtttagatc atatggcatg
      181 caagtaaata atttttaccc atggttaact ctatatcctt tatttttatt gatcaagttt
      241 actaatttag caataggcta taaccttttt ctttatattg ttacgctcat tactctattt
      301 atatgtcatt atgtaatgta tgaaattacc aaaaaacatg ttactagctc ctttttcgct
      361 attatatata caacatcttc ttttaggagt gtagaaatat ttcttcgagg ggcaatgggg
      421 gaattactcg caatgtcaat acttcctctt attttactcg ggtttataaa gctttacgac
      481 tcaaaaaaag aatcttgggt tatgttggcg attagcatga ctttattaat ttacactcat
      541 gttctttctg tagctatgac tatggtaatg attataattg ctttaattat tcaattttat
      601 cggaaaaaga aaatagaaat cttcttaatt attaaattaa taaaagctgc tatagttact
      661 ttacttcttt cactagcgtt tataggtcct atgattgagc agacattgta tcaagatctg
      721 aatagacctt atgtggatat attacaaaaa agagcaattt atctgggtaa agagtttttg
      781 attggaagta tagattcaga attactatca tttggtattg gcttaacttt gcttatttgt
      841 ttaattatac taacatttaa ctttaaaaaa ttaggaatct tatctaaaat cacttatata
      901 atgggtataa tatctattct actttgtaca aaaatatttc catggtttgc cttacaaaat
      961 actcctttaa atattattca agctccttgg cgttttatga tattttcaac attgttcata
     1021 tctttttcag tatctctatt tatttctcca cagttggaaa aattttctta tacaaggaga
     1081 agaaatttt
//
LOCUS       DAZKAS010000299         1086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000299 DAZKAS010000000
VERSION     DAZKAS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.311"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..196
                     /locus_tag="ACG8H7_002938"
     CDS             47..196
                     /locus_tag="ACG8H7_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984961.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            193..639
                     /locus_tag="ACG8H7_002939"
     CDS             193..639
                     /locus_tag="ACG8H7_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984962.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            641..778
                     /locus_tag="ACG8H7_002940"
     CDS             641..778
                     /locus_tag="ACG8H7_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFP8984963.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            815..>1086
                     /locus_tag="ACG8H7_002941"
     CDS             815..>1086
                     /locus_tag="ACG8H7_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984964.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          397 a          179 c          220 g          290 t
ORIGIN      
        1 gcttattcat ttgttatcac ttattcaatt taagggagga aatctaatga aagtagttta
       61 caaatcaatc aagccttacg gattcgagca aatcattgtg aatgacgaag aacatttacc
      121 ggaagaatgc acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg
      181 ggagggaaat aaatgaaaaa catttggaaa tatggccgta ctggcggaga gtatgcagga
      241 aaagtattgg acgacatgct tgtatccgtt ccttacacag atcagccacc gctcgaaggg
      301 gttcgttcag atggcgaacc gctaacgatt gctgatcaga tgtttgatcc taaattgaac
      361 caatggatta ttttagcgaa cgcgttagat cacaacgatt taaacaatct caaagcgatg
      421 tacgaggctc ttgaacatga aaacggcaac ctaaaacagc taaatgccaa actcatgcta
      481 agcgatgtag cgattaaaca ggaaaatact gcattgaaag aaaaagctga cagtttagca
      541 caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt
      601 aaagagcaac taaatccagc ttcaaaagga ggtgagtagt atgtttagtt ttagcgatgt
      661 gaaaatgatg tatgattggg gctgttttac tgacgatcaa gttcgactat tcgttccact
      721 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
      781 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
      841 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
      901 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
      961 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1021 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1081 aacttt
//
LOCUS       DAZKAS010000300         1083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000300 DAZKAS010000000
VERSION     DAZKAS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.312"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..409
                     /locus_tag="ACG8H7_002942"
     CDS             2..409
                     /locus_tag="ACG8H7_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP8984965.1"
                     /translation="MKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKI
                     LAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILI
                     GFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            423..779
                     /locus_tag="ACG8H7_002943"
     CDS             423..779
                     /locus_tag="ACG8H7_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFP8984966.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          376 a          163 c          207 g          337 t
ORIGIN      
        1 gataaaagta tatacttcgc ctagctgtac ttcctgtaga aaagcaagag catggttgca
       61 agcgaatcaa cttgaatttg aagagaagaa tatttttgcc gaaccgttaa cagaaagtga
      121 gatcaaaaag atcttggcag cgactgaagg aggagttcaa gatattattt ccactcggtc
      181 gaaaatttac gaaaagctgg gcatagattt taacgaatta actttaaagc aaatgatcgt
      241 cttattcaaa gaatatcctt cattattgag acgacctatt ttgattgatg aaaaaagaat
      301 actaatcgga tttaatgaag atgagatccg gtcatttgtc cctcgtgaaa ttcgatgtgt
      361 tgccagaaat ctgatctatt tacaaaacaa taacatctta aattcatagg gagggagcct
      421 tcgtggaagt gaaagtctat ggatcagcgc actgtgtaac aagtctgaag gcgaaacaat
      481 ggttagaaca ccatcaattg ccttatcaat ttgttgactt agaagaacgc gatctaagta
      541 cagaagaagc agaagaatta tgcagttttg aagaagtgca agcagaacag ctatttgcga
      601 cttggtctga ggcttttcac aacattgcga ttgacctttc ctgtgtagga aaaaaacaac
      661 tgattttttt atgcacacaa cataccaatc tcctcagacg tcctttgatt attatcgatg
      721 aagttctatt tattggatac aatgagcagt tgatggaaca gcttatctta aataaataga
      781 ctttaactgt tttaatcaag tcactgagca tttcatacag ttgtaacagt cgaacactta
      841 gcatcaagcg tgttatgtgt tctttttttg actttatttt tctactatag agacatgctc
      901 aatttatgtg gtcaaaattc aaatctaggt ggggatatag agttaaaaat atattgacat
      961 gtgtaaattt acatgatata ttcatttctg ttgttttaag tagcaaagca ataaaatagt
     1021 attttaaaaa acatataaaa tagttgttga catgttagaa atgacatgat aagataactg
     1081 agt
//
LOCUS       DAZKAS010000301         1063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000301 DAZKAS010000000
VERSION     DAZKAS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.313"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..846)
                     /locus_tag="ACG8H7_002944"
     CDS             complement(37..846)
                     /locus_tag="ACG8H7_002944"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFP8984967.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          322 a          202 c          186 g          353 t
ORIGIN      
        1 tgtcacaacc tcttttttgt ggaatctaat tctctattat agtttaagta gtttttccag
       61 ataaacagct aaaccgtctt cctgattgct caaaggagtg acatcatttg caatgccttt
      121 gagttggtca gtaccgttag ccatagctac tccccaacct gcataatcca acaattccaa
      181 atcattatgc tcgtcaccaa aggcaatgac gtctttttga tcgatagaca ggtagttagc
      241 aatctcttgc acgccttttg ctttttggat tccttttgaa acaatctcta agatggcagt
      301 aggtccaccc catgtccgaa catcgacata actgccaaat tgacgtgtta attcagcaga
      361 tacagctcct gcaaaacgtt tgtctgttcg gacaagcaaa gatgtaggat ttgtcgtcaa
      421 atttttagga gaaagcaaat tcttctctcc tggacgagaa gaagcaaaaa ttttttcatc
      481 aaagaaatca aaactgtcaa taaaaaaagt atcacgattt tcagcagcga taaaatctaa
      541 atttagctgc tttttttgcg ataagatttc aaaagcgatt tcacgattga tcaacgtttc
      601 tctttcacca tcccagtgct gattagggat gtgtactaat gcgccgttaa aattaaccat
      661 cggtgttgtt aattctaatt gttggtaaaa ctgatggctc attcggaatg ggcgtcctgt
      721 agcaatactg acatgatgac cggcattgat tgcttttttt agagtctctt ttgttttaga
      781 tgtaatcatc gaatcttgat tgagggtagt tccgtccaga tcgatggcaa ttaatttttt
      841 attcaataag ggtccttcct tcctcaacta gcatttgttt tagtctagca tattttaggt
      901 aattaacaag agcaatcagc tattcctaac aaaaactaca gttatggcta tataaatagc
      961 taaacataag ctaaacacga acgttaagta ttattttcgc tttttttgtt aaaaaaacgt
     1021 tgaaaagcga atgaaacgtc tatataattt gaaattgttc gat
//
LOCUS       DAZKAS010000302         1062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000302 DAZKAS010000000
VERSION     DAZKAS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.314"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACG8H7_002945"
     CDS             complement(37..>1062)
                     /locus_tag="ACG8H7_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFP8984968.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZKAS010000303         1049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000303 DAZKAS010000000
VERSION     DAZKAS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.315"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..735
                     /locus_tag="ACG8H7_002946"
     CDS             43..735
                     /locus_tag="ACG8H7_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP8984969.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          462 a          119 c          156 g          312 t
ORIGIN      
        1 taataactta tttaaggaaa ttttttgaag gagaagataa aagtgagaaa aaagattatt
       61 attaatgctg gagtaattat aggattgatg ttagtaattt tctccggaaa gcaatatgct
      121 gataatattt tattatgggg aggggaagat aatattgaaa agatcaacaa caatttaact
      181 atattagaca cagctctaga aaataaagag caaaaaatta gtattttaaa ttcacaattg
      241 tcttcaaaca accaaactct tgaacaacta agaactaata ttgaagtttc caaacaaaaa
      301 gtggcatctt tagaaaacga caagaatcaa ttagtatcag agaaaactaa tttagaaaat
      361 caattgtcag caaaagactc tgaattgcaa ggaaaacaaa atgaaattaa tgcgaaaata
      421 gacgaaatta accaaaaagt agctgaaata aatcgattaa atagcgattg gaataatcgt
      481 ttacaaaatg aaaagaacaa atttaatgaa gaacaatcta ctattaatag tttaaaccag
      541 caaattaatc aaatgttgag tgaaaaaaat aacattcaaa atcaactgca agctactcag
      601 caaaagctag aagaagaaaa gtcaaaaaat agtagtttga ataattacat tgaaaagctt
      661 caaaaagcta aaaatgatgt agaggatacc gcaaataaag cacaacaaat tgtaaatgaa
      721 catacaatcc aatagatata gttttaattt aacaaatggt atgtttggag ataatgagat
      781 agaatataaa tgtcataata ttatacaatc taaacaccgt ttccttttct atttttgatg
      841 acatcagtat atagctactc gaaagagtag tctttttatt attctcgctt attattgata
      901 tgaaaatttc gatttgacaa aaggtaccga ataattttgt aatatgaatt attatcaaga
      961 agtaattatg atgataacat atggaaacta gttagtaata ataacaggtg attaaacttt
     1021 tgaacgactt gtaattctat attttttct
//
LOCUS       DAZKAS010000304         1048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000304 DAZKAS010000000
VERSION     DAZKAS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.316"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..438
                     /locus_tag="ACG8H7_002947"
     CDS             88..438
                     /locus_tag="ACG8H7_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFP8984970.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            537..>1048
                     /locus_tag="ACG8H7_002948"
     CDS             537..>1048
                     /locus_tag="ACG8H7_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HFP8984971.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRI"
BASE COUNT          300 a          167 c          199 g          382 t
ORIGIN      
        1 ttgttttttt gaataatcgc tttttcttct ctttttctta tatactataa gtagtaaata
       61 atttgaaaaa aagaaaggtg actgattatg gcgcaaaatc gtatttttac gacaagtttt
      121 gcaagcgttt atcctatgta tgtagcaaaa gcggagagaa aagatcgtat aaaagaggaa
      181 gtagatgaaa tcatttgttg gctgacgggt tattcccaaa cagaactaga atatcaacta
      241 caaaaacaag tgactttcga acaatttttt gcggaagccc ctgtccccaa tccttcacgt
      301 tccttgatta caggtgttgt ttgtggtgta cgagtagaag acatcgagga taacttgatg
      361 aaagaaattc gttatttaga taaaatgatc gatgagctag caaaaggcaa aaaaatggaa
      421 aagatcctca gaagttgatt tttccagaga tattcttctc cttcgctcat tttgtaacat
      481 ttaatttggt aaaatgacta gttcacgtta aaataagtca aaggaggttt tcttatatgt
      541 ttgagacgat taaacaaaat attcaaaaat acgccttatt aagagctgct gtttatatta
      601 tttctgggat tgcgattgtg ctaaaccctt ctgctgtttt tcattttatc ggttatctga
      661 tcaccgccta tcttgttttg cttgggttgt tgaatctttt tgaggcttac cgttttaaaa
      721 ggaaaacagg ttcttggagt atcggtctgt tcagtggtct ttttttcctg atcttagctc
      781 tgatcgtcta tgcttttgca cctgcaatcg tttctatcct gccgattctt cttggattag
      841 ctgttatcat caacagcttg ttccagttgt tcttcagcat gaatacaaaa gctaaaagtt
      901 ggagtgttta tagtagtatt ctgttgttga ttggcggatt cgtcttattg ttcaatccat
      961 tcaaaagttt gatgcttcta ttccaaatat tcggctttat tttgattttc atgggcatcc
     1021 aggagattat caatttcatc agaataag
//
LOCUS       DAZKAS010000305         1024 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000305 DAZKAS010000000
VERSION     DAZKAS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.317"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..203)
                     /locus_tag="ACG8H7_002949"
     CDS             complement(<1..203)
                     /locus_tag="ACG8H7_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFP8984972.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFN"
     gene            879..>1024
                     /locus_tag="ACG8H7_002950"
     CDS             879..>1024
                     /locus_tag="ACG8H7_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP8984973.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QLDQL"
BASE COUNT          378 a          160 c          127 g          359 t
ORIGIN      
        1 tcattaaaat caccagtaaa gaaatggaaa tctttagttt ttttaatatt atcagttagt
       61 aactttttat aatcataaaa aaaataagta gttctatctg agcgatactc ttttataata
      121 ctgaattcgg aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat
      181 aagacttctt tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag
      241 tattttatac gttatctaaa atactattcc tctgtcaaaa taagctaact tacttcatta
      301 taaaatatcc acttatcgct ttcatgtata ttccaccgta aaaagtacat tttatcaccg
      361 agaactcatc atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat
      421 aattattatg atccgttatt atctaagcac tatacagata ataggaattt ttcgtttcta
      481 aatattccaa tttgggaatc caataaaaat tttcgttcaa atacgtgtct attcgaactc
      541 ttttctagtt gttaattatg aagggggaag aaataaaaaa tacaatctat acacgaataa
      601 gaatattatc caaaaggagg ttttagaaaa gatttattat acgtaaaaag ctcaagacat
      661 tttcttagcc ataaggacat gtttgatatt taagaagata tatgaatttt cttttctaag
      721 tataaagata actatccatt atcaactaaa aaaacatttt tgaaaataaa catcagcagt
      781 cctatcaatg catgtgagct tatagaagtt attcgcagcg cagcataatt gcattagctt
      841 cttttcgtgt tttttgcctc ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt
      901 cagataacga cgaacgcgta aatgcccagt tgattcgatt ctttgaaaag gtcattttga
      961 acaccgctaa tacttattat aaaaaacaag aagaaattca aaaacatgaa caacttgatc
     1021 aact
//
LOCUS       DAZKAS010000306         1015 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000306 DAZKAS010000000
VERSION     DAZKAS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.318"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..>1015
                     /locus_tag="ACG8H7_002951"
     CDS             3..>1015
                     /locus_tag="ACG8H7_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFP8984974.1"
                     /translation="MFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESKSFDD
                     NIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACLYRNN
                     KNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGR
                     ENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCK
                     IADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSG
                     SVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAES
                     LDD"
BASE COUNT          390 a          116 c          182 g          327 t
ORIGIN      
        1 caattttttc cgatgagtat gttgaaatta ttatttctga tcattctaaa aacactaaag
       61 aaattgcgaa aaaaatagaa gaaaaaaaag tgtttgatgg ctgtcattat ttagaaagca
      121 aatcttttga tgataatatt gggtattttc aaaagattaa ggactgtctt cagatttctt
      181 gtggtaaatc aaatcgatat gcctattatt tgaaaaactg taaaagtgaa tattttgatg
      241 aatttatttg tttttcaatg ggagaggttt ggttggaagg aatatatgct tgcttatatc
      301 gaaataataa aaatgtacag gtatcaattt tagaagaagg tattttaagc tatggagttg
      361 acattattcc aacagcaaga acaaaagcta tcgcagtact caattactta aaaaaaagga
      421 ctccactata taacaaaagc tataattttt attgtttttt tcctaatata tatacaggaa
      481 gagagaatac aatacaaatt ccattagtta cagctgggtc aagaaccgca ttgcaacttt
      541 cggatatttt taaccctatg atagatattt ataaagaaaa aaaatatata ttttttacgt
      601 ctgtttatga ttttgagggc ggagaacctg tcggagaata tgaattagta tgcaaaatag
      661 cagacttagt aggaaaagat aatctaattg ttaagacgca tcctagagat gtaagaacaa
      721 tttataaaga taatggattt atcgaggata aaaattcatc aataccttgg gaggcgattc
      781 aattgtcagg cgattttaac gataaaatat ttatgacaat taattcagga agtgttattt
      841 caggtagtac aatgtctgca aaacctgtac gcacgtatta tatgtataaa ttgtgtaaca
      901 tagaaggaaa tccgtcttgc aaaaagaatg cgtatgatat tgagcgattg cttaagcaaa
      961 aaacgatgaa aaacatttta aagaatgtga aaatagcaga aagcttagat gatat
//
LOCUS       DAZKAS010000307         1014 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000307 DAZKAS010000000
VERSION     DAZKAS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.319"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8H7_002952"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8H7_002952"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP8984975.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          264 a          223 c          159 g          368 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgct
//
LOCUS       DAZKAS010000308          997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000308 DAZKAS010000000
VERSION     DAZKAS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.320"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACG8H7_002953"
     CDS             complement(26..913)
                     /locus_tag="ACG8H7_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFP8984976.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DAZKAS010000309          987 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000309 DAZKAS010000000
VERSION     DAZKAS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.321"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            354..987
                     /locus_tag="ACG8H7_002954"
                     /pseudo
     CDS             354..987
                     /locus_tag="ACG8H7_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          337 a          181 c          180 g          289 t
ORIGIN      
        1 agaaaatgta gttatatatt taaattttta ccagaaagaa atgaacacct tcaattatct
       61 aaatctcgaa aatggttaga tgtaatcatc aggaaaaaca atcttaaacg aattactatt
      121 catggtctta gacacacaca tgctagcttg cttttagcaa gctagtgcaa atattaaaga
      181 tgtgcaagag cgtttaggcc actcttctat tcaaatcatt atggatctat atattcatat
      241 tacagacaaa agaaaagaag aaaccgtagc gcaattcgca aaatataggc tctataattt
      301 atgggactga tgaatcaaaa ttgattcttc agtcccattg ttattttagg tatatgattc
      361 gtacattgaa agagttgaaa aactatcttc cgaagccgtc aaaaaggacc ctgccaaaag
      421 tattgatggt ggatgaattt cgttcgcacg cttcaattgt ggataaaatg agctttattt
      481 gtgcggatgg cgagactgga aaactcattg atattttgcc tacacgaaaa ttgtctcgat
      541 taacacacta ttttagtcgc tgtgcaaatc ctgaagcggt ggaatattta gtgacagata
      601 tgaacgccgc ctacttccaa ctgacaaaac gtgtcttacc aaatgcgaaa gtcgtcattg
      661 attgttttca tatcatcaaa catttgaatc aggcgttcaa tgattttcgt gtacaagaaa
      721 tgaaaagatt acaccagtta gggcaacgac gggaagccga gaaagtgaaa aagaactggc
      781 gctttcttct aaaaaaccgt caaaaaatca atcactatga ttacaaaaca tggcgcagtt
      841 ttcgtgcacc taaatacccg ctactcactg aagcaatgat gattgatcga ttgctggcat
      901 tttcaacgcc tttaaaggac gcctatactt ttttccatga gctgaccgaa gcctttagag
      961 acaaggaatc agacttattt tttacct
//
LOCUS       DAZKAS010000310          985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000310 DAZKAS010000000
VERSION     DAZKAS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.322"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACG8H7_002955"
     CDS             <1..704
                     /locus_tag="ACG8H7_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP8984977.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          328 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataa
//
LOCUS       DAZKAS010000311          979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000311 DAZKAS010000000
VERSION     DAZKAS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.323"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..274
                     /locus_tag="ACG8H7_002956"
     CDS             50..274
                     /locus_tag="ACG8H7_002956"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP8984978.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            376..609
                     /gene="relB"
                     /locus_tag="ACG8H7_002957"
     CDS             376..609
                     /gene="relB"
                     /locus_tag="ACG8H7_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFP8984979.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            599..868
                     /locus_tag="ACG8H7_002958"
     CDS             599..868
                     /locus_tag="ACG8H7_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP8984980.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT          347 a          157 c          205 g          270 t
ORIGIN      
        1 tcgcaatccc taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa
       61 ccaaaatacg aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac
      121 tgatactact gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat
      181 ccctgaaaat tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag
      241 attttgcgat accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt
      301 tgtactaacg ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg
      361 aacggaggta gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa
      421 gaactggtat tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat
      481 gctgtactgg ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa
      541 gtttccaaag agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat
      601 gaattataaa gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa
      661 ccaagctaaa ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg
      721 tcagcacgga aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa
      781 ctatcgaata ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg
      841 acatagaagt acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt
      901 caatgtataa tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg
      961 gttggtttct gctttttta
//
LOCUS       DAZKAS010000312          972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000312 DAZKAS010000000
VERSION     DAZKAS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.324"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..>972
                     /locus_tag="ACG8H7_002959"
     CDS             100..>972
                     /locus_tag="ACG8H7_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8984981.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          340 a          168 c          179 g          285 t
ORIGIN      
        1 ttattacgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
       61 aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      121 acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact
      181 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
      241 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      301 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      361 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      421 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      481 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      541 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      601 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      661 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      721 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      781 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      841 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      901 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      961 aaacgaaata at
//
LOCUS       DAZKAS010000313          959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000313 DAZKAS010000000
VERSION     DAZKAS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.325"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACG8H7_002960"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACG8H7_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACG8H7_002961"
     CDS             complement(570..>959)
                     /locus_tag="ACG8H7_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8984982.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAZKAS010000314          944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000314 DAZKAS010000000
VERSION     DAZKAS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.326"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..>944)
                     /locus_tag="ACG8H7_002962"
     CDS             complement(114..>944)
                     /locus_tag="ACG8H7_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP8984983.1"
                     /translation="SKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLL
                     FANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIE
                     SNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSD
                     AKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTF
                     FRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN
                     "
BASE COUNT          366 a          158 c          112 g          308 t
ORIGIN      
        1 caaattcaga ataaataaat aaatctcctt ttgattctcg gtaaaaattt tgataaacat
       61 ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa agctgtcttc atattaattc
      121 tcctttttta aatacaagta agaaatgctt cccaaataat accagaaaac aattgaaatg
      181 acatcgcgct tattgaaaag tacatgagcc tccaccattt catctataaa aatagatagt
      241 ataaaaatta aaacaatacc aagaaacgca taattcactt tacttctaaa aaatgttttg
      301 agtaaactaa agatatttaa cacatagaaa gcatagatac atataaaagc aattatacca
      361 cttgatacaa gtacttgaag aaatccatta tgcatgtttc catgtgctct ttttagttca
      421 ttcacatctg tagtatcaag atctactttt ttcaaaaatt ttgcatcgct attcccatag
      481 aaatcggtct ctccataacc aaataacaaa gttggtctta taactttaag cgcagctttc
      541 caaatcgtaa aacgaccagc tgaaatttcg gagttatcac tagaatgctg taatattact
      601 tctttttctg gaagtggagt tttatctgat tcatttgact ctataacttc tacttttcta
      661 tcaatcgata caggaattgt tgctaaaaaa tccttagcat aattagtacc aaaagaaaaa
      721 tataccaata gaactaataa tatgcttacc atccatttga tttttacagg tagtacttct
      781 ctaatgctct ttctaaattt tgaattggca aacaacaaaa gaaataatac taaaaaagaa
      841 caaattacta cctgggcacc tctagacgaa gaaagtgtaa tataaaatat ttgtacgata
      901 aaattcatca tataaaaaac acgaatcgtt tttgttattt ttga
//
LOCUS       DAZKAS010000315          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000315 DAZKAS010000000
VERSION     DAZKAS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.327"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>931
                     /locus_tag="ACG8H7_002963"
     CDS             <1..>931
                     /locus_tag="ACG8H7_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFP8984984.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAZKAS010000316          921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000316 DAZKAS010000000
VERSION     DAZKAS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.328"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>921
                     /locus_tag="ACG8H7_002964"
     CDS             2..>921
                     /locus_tag="ACG8H7_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP8984985.1"
                     /translation="MFSVELPVIGVLVLVAVFIISKVNLIFLIFSLIMMGAMVLQYNL
                     FQLPLKKTIENTKSVNQSITGELNAHFDQLEIISAYNFENISILKFDSRFKELFNVFK
                     KQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISITNFVIVFMYANMVPTLWTT
                     LISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSITGQSLNFSYNKRKVLSNI
                     SFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLIS
                     IATQIPYIINSSVKENILMGTKNYDKKKFS"
BASE COUNT          360 a          103 c          140 g          318 t
ORIGIN      
        1 tattttttct gttgaattac ctgtgatagg cgttttagtt cttgtagcgg tatttattat
       61 aagtaaagtg aatcttattt ttctgatttt ttcactaata atgatgggag ctatggtgtt
      121 acagtataat ttatttcaat taccgcttaa gaaaacaatc gagaatacaa aaagtgttaa
      181 tcaaagtatt acaggagaat tgaatgcaca ttttgatcaa ttagaaatta taagcgcata
      241 taattttgag aatatctcta ttttaaaatt cgattctaga tttaaagaat tatttaatgt
      301 tttcaaaaaa caaaataaat tactaacact gggaagctgt ttggcacagg ttattttcca
      361 attatggatt ggaggaatat ttcttattga tgttatttta ttagctaaag gaacaataag
      421 tattactaat tttgtaatag tatttatgta cgctaacatg gtccctactt tatggactac
      481 tttaattagt attatttgga aatggcaaga ctattcagtg tcattaaata gatattacga
      541 atattattca aataacaaca aagtggctgg cacaaaaaaa ataaataaaa taaattctat
      601 tactggtcaa tcattaaatt tttcatataa caaaagaaag gtattaagca atataagttt
      661 ttcaactgaa atgaataaac tattcatgat atcaggagaa aatggaacag ggaaaacaac
      721 actgatgaaa ttatttacta aacagttgcc ggttagtaat aatcaattat tcgtcaacgg
      781 gatagatatt aatatgattg ataacgaatc attaagaagg ttaatttcaa tcgcaacaca
      841 aataccatat ataattaata gctccgtgaa ggagaatatt ttaatgggga ctaaaaacta
      901 tgataaaaaa aaattcagta a
//
LOCUS       DAZKAS010000317          916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000317 DAZKAS010000000
VERSION     DAZKAS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.329"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>916
                     /gene="bsh"
                     /locus_tag="ACG8H7_002965"
     CDS             6..>916
                     /gene="bsh"
                     /locus_tag="ACG8H7_002965"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFP8984986.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKED"
BASE COUNT          315 a          123 c          167 g          311 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agactt
//
LOCUS       DAZKAS010000318          913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000318 DAZKAS010000000
VERSION     DAZKAS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.330"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..226
                     /locus_tag="ACG8H7_002966"
     CDS             <1..226
                     /locus_tag="ACG8H7_002966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984987.1"
                     /translation="KNEKSWHLAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKN
                     ENFFVSVLMGESLILILILCLIVHEVNKQL"
     gene            650..913
                     /locus_tag="ACG8H7_002967"
     CDS             650..913
                     /locus_tag="ACG8H7_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984988.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT          359 a           95 c          162 g          297 t
ORIGIN      
        1 gaaaaatgaa aaaagttggc atttagcaca aaaattgttg ggggattttt caaaaaaaaa
       61 agttatccca gtatttttga tgggtattgt ttttttcagt atagagattt atagtttagc
      121 tattttaaaa aatgaaaatt ttttcgttag tgtattaatg ggtgaaagtt taatcctaat
      181 actgattttg tgtttaattg ttcatgaagt gaataaacaa ttgtgaatta aaaaaataaa
      241 aacctagtat aatcacttta ataaattgtt ggtagaaggt gatcaaaatg ggagtaaacg
      301 aaatgatgaa agtcagattg agtttttaaa tgataccttc ctcagaactt caaaacgaat
      361 tcaaagattc gttgacaaat cttgagcaag atcatttaaa aaaatatttt ctcttcattt
      421 gacgaaaaat gtttagaaat cttatataag tgaagataaa gtaattggtg taattaattg
      481 tagagaatag cctttgagca attgaagcga tacaattatt tagaaaatga acttcctcca
      541 gcatactaca tgattcctta cgggacgatg ttaaagaaga aagcaagaaa ttttagaagt
      601 attagtagat aaactaatgg gcttttctca atttaaggga gtagcccaaa tgtttatcaa
      661 atatggtgca gatacagtag caaccatgtt tacagaagaa tatcaagtag actattatga
      721 agtaatagat aaatgtgtac attttgttta tgaaaaagtg tggaatttta tgaattttgc
      781 gataagtgaa gaaatcttac gagtacaaga aaacagaaca ggaatattta ccagagacag
      841 aaagcttaat ttgattgcgc attatatacg gaatcgctgg tcatcaatac caaattttaa
      901 ttataaaaaa taa
//
LOCUS       DAZKAS010000319          904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000319 DAZKAS010000000
VERSION     DAZKAS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.331"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACG8H7_002968"
     CDS             265..888
                     /locus_tag="ACG8H7_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP8984989.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAZKAS010000320          901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000320 DAZKAS010000000
VERSION     DAZKAS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.332"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>901
                     /locus_tag="ACG8H7_002969"
     CDS             <1..>901
                     /locus_tag="ACG8H7_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFP8984990.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPE"
BASE COUNT          305 a          154 c          188 g          254 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 g
//
LOCUS       DAZKAS010000321          901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000321 DAZKAS010000000
VERSION     DAZKAS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.333"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..356
                     /locus_tag="ACG8H7_002970"
     CDS             87..356
                     /locus_tag="ACG8H7_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP8984991.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="ACG8H7_002971"
     CDS             349..606
                     /locus_tag="ACG8H7_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFP8984992.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          345 a           98 c          172 g          286 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgcttata tatatgactg aatagaataa
      901 g
//
LOCUS       DAZKAS010000322          895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000322 DAZKAS010000000
VERSION     DAZKAS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.334"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..500)
                     /locus_tag="ACG8H7_002972"
     CDS             complement(102..500)
                     /locus_tag="ACG8H7_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP8984993.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(497..733)
                     /locus_tag="ACG8H7_002973"
     CDS             complement(497..733)
                     /locus_tag="ACG8H7_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP8984994.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          283 a          152 c          131 g          329 t
ORIGIN      
        1 tatttttatg aaaaaactat tttgtatgat taaacataaa aaaatcagca tattccttaa
       61 tgacatgctg atctaattga gaaataaagt ttttaattcg ttcattttat tgcggtttgt
      121 cgtaaataac tggttatctt ggactttagt gaatcgaaat ccaatgaact agtggtaatc
      181 attatgataa aatcaacagc ttcttgacga gaaaaagcaa ctggacaatt attcaattga
      241 agaaacaaca acatggcaac aagagcagtt cgtttattgc cattatgaaa aggatgtttt
      301 ttagctaaat taattgctaa aatcgccgct ttttcataga tttcaggata gagttcttga
      361 ttgaatactg cttgtgaagg ctgtttgacg gccatatcta atgcatttgc atcttttaca
      421 ccgatcattt cgcctggcga gtgacgtaaa ataactttcg cgttgatttt gataatttct
      481 ttagtcgaaa gatacctcat aaatcaacca aatcttttaa cgcgtcttca tattgattca
      541 tgatcgaatc aactaatgaa tcaatatcag gttcttcttg tgctttgatc attcgtacag
      601 aaccatcaga ttgaaaaaca taactgattg cgtcacctgt ctgcacaccc atgaattctt
      661 ttacttctgg tgggatagtt gttacatcgc tattgccaga ttttctaatt ttatactttt
      721 tcattttttt catatttcgc caccctctat accattctca ttctatagta agtacattat
      781 atccaagtat atacttttaa tcaatgtata tacttttatt ttgctttttc ttacgaaaaa
      841 atgacaaaga aaaaagtttg tgacatgact tttgtcacaa actctcgtat tctta
//
LOCUS       DAZKAS010000323          888 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000323 DAZKAS010000000
VERSION     DAZKAS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.335"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..358
                     /locus_tag="ACG8H7_002974"
     CDS             <1..358
                     /locus_tag="ACG8H7_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP8984995.1"
                     /translation="FFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRRFPEGEE
                     VWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDDWQKVMA
                     AEVIATYLDGDLVYKK"
     gene            406..>888
                     /locus_tag="ACG8H7_002975"
     CDS             406..>888
                     /locus_tag="ACG8H7_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP8984996.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     D"
BASE COUNT          260 a          148 c          206 g          274 t
ORIGIN      
        1 cttctttaat tattgtcgat tagaggaaag tggtgtaaca gtcactagcg gtacagattt
       61 accgctgttt attactgata ttcctgaatc gattataaga gcagtattcc gtcgttttcc
      121 tgaaggagaa gaagtctggg agaaagaaac aagcttcacg acaaccgagc tgctgaaaag
      181 ttttacaagc aacagcttca aagtaaatgg tttatcggag tacggagaaa taaaagtagg
      241 gaaaagcgcc agcttagctg ttttcagcga ggacctctgt actgatgact ggcaaaaagt
      301 gatggctgcg gaagttatcg ctacctatct tgatggtgac ttagtttaca agaaataatg
      361 cttttcgata taacgttata agacgaatta aagagaggtt aaaaaatgga aacaatgaaa
      421 agaaaattca ccttcccaca tagttataca ttgattttta tcttaattat tttggctgca
      481 attttaactt ggataattcc tagtggtcag tttgagacag ttgttgaaaa cgtcgcaggt
      541 atggaaaaaa caacggttgt tccgggaacc tatcaccaaa tcgataaggc agattatgga
      601 caaggttttt cctctatact aatggcacca gctgaaggga ttatttcagc tgttgaggtt
      661 gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta tcctaaaaac gggagccatc
      721 gatcgagggc tgtttacctt agcaaaagtt ctgggagaca aggggatctt agttattccg
      781 atgtcgatga ttctattcag cttaggagga tcaacctttg gcatgagcga ggaagtcatt
      841 cccttatttg cggtgtttat ttctctgatg ttttctcttg gctatgat
//
LOCUS       DAZKAS010000324          876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000324 DAZKAS010000000
VERSION     DAZKAS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.336"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..75
                     /locus_tag="ACG8H7_002976"
     CDS             <1..75
                     /locus_tag="ACG8H7_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP8984997.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDDNE"
     gene            253..594
                     /locus_tag="ACG8H7_002977"
     CDS             253..594
                     /locus_tag="ACG8H7_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8984998.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            587..>876
                     /locus_tag="ACG8H7_002978"
     CDS             587..>876
                     /locus_tag="ACG8H7_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP8984999.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          325 a          142 c          185 g          224 t
ORIGIN      
        1 ccagaaactg cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt
       61 gatgacaatg aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg
      121 gaaatactat aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca
      181 acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg
      241 agattttagc atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat
      301 tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg
      361 aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat
      421 gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa
      481 gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat
      541 tacggaccag gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa
      601 agggacaacg aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa
      661 aggaaatttt gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc
      721 acaggcaacg gtaactatta atgagtacaa acactacagg gttgtttctg gaactcatca
      781 agactttctt gttgaggaat ataaaccttt tcttatggat gaaactggga cttatgtatc
      841 tgtctcagta acggttaaag gacacacgga aaccga
//
LOCUS       DAZKAS010000325          875 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000325 DAZKAS010000000
VERSION     DAZKAS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.337"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..544)
                     /locus_tag="ACG8H7_002979"
     CDS             complement(<1..544)
                     /locus_tag="ACG8H7_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP8985000.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVL"
     gene            complement(620..766)
                     /locus_tag="ACG8H7_002980"
     CDS             complement(620..766)
                     /locus_tag="ACG8H7_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFP8985001.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          355 a          137 c           93 g          290 t
ORIGIN      
        1 atagaacaac aaacatttga atgattaaaa atattaagac accaaacaca aaatgatcaa
       61 atacttgata ttgatgagca acataagaaa caaataaacc aattgtaagt aaataaatca
      121 tgaatatagc agtaagttta gaactggact gttcaacaat cctatttata gctaattttc
      181 tatataacca aataaacatc agcaaaaaca aagaatcaag cattatctgg aatactacat
      241 aaccataccc ttttatccct tcaaatgaaa acatatacgt cataaccatg gaagtaataa
      301 tagatctgaa atcatcaata agcatacata atagtaattt atttaaaagc actatattag
      361 ttttcttctt ttcctttaat ataaaataac ttccgattat aggtatagct gcggaaacat
      421 cagtaaataa ttcaacaaaa aaagtgagtg gtatcaataa acatacaaaa agatattttc
      481 ttttgttaag atcagtatta gtcatataaa ataagagaaa aaagaaaaag tttcccacga
      541 acattcttaa tagataagct tttgtatcga tagtaatcaa gagatcccct cctgatttta
      601 ggatatcata aaaaaaattt tagttacact ttttaaataa tgaaaaaaca cattccacta
      661 aatttgatgc cggttttcct tgaggttttg ttccagcacc tcctttaatt tttttaagct
      721 cttgatctga acattgttta gcatttagtc tatttttttc ttccattacg attcccccta
      781 caaactataa atacttttat gtcactgatt caatatacct atttttctaa tcaaaaaatt
      841 ttgagacata tttaatatta taagtatcat taaaa
//
LOCUS       DAZKAS010000326          853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000326 DAZKAS010000000
VERSION     DAZKAS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.338"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8H7_002981"
     CDS             136..309
                     /locus_tag="ACG8H7_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985002.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          284 a          142 c          104 g          323 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ata
//
LOCUS       DAZKAS010000327          814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000327 DAZKAS010000000
VERSION     DAZKAS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.340"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG8H7_002982"
     tRNA            8..81
                     /locus_tag="ACG8H7_002982"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="ACG8H7_002983"
     tRNA            121..210
                     /locus_tag="ACG8H7_002983"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="ACG8H7_002984"
     tRNA            225..296
                     /locus_tag="ACG8H7_002984"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="ACG8H7_002985"
     tRNA            312..384
                     /locus_tag="ACG8H7_002985"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="ACG8H7_002986"
     tRNA            394..466
                     /locus_tag="ACG8H7_002986"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="ACG8H7_002987"
     tRNA            502..577
                     /locus_tag="ACG8H7_002987"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="ACG8H7_002988"
     tRNA            579..659
                     /locus_tag="ACG8H7_002988"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="ACG8H7_002989"
     tRNA            666..739
                     /locus_tag="ACG8H7_002989"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
     gene            742..814
                     /locus_tag="ACG8H7_002990"
     tRNA            742..814
                     /locus_tag="ACG8H7_002990"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:His,seq:gtg)
BASE COUNT          178 a          187 c          224 g          225 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg gattgtggct
      781 ccggcattcg tgggttcgat tcccatcatt cgcc
//
LOCUS       DAZKAS010000328          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000328 DAZKAS010000000
VERSION     DAZKAS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.341"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8H7_002991"
     CDS             <1..672
                     /locus_tag="ACG8H7_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFP8985003.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKAS010000329          803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000329 DAZKAS010000000
VERSION     DAZKAS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.342"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..>803
                     /locus_tag="ACG8H7_002992"
     CDS             129..>803
                     /locus_tag="ACG8H7_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP8985004.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNA"
BASE COUNT          240 a          155 c          147 g          261 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagcag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac gct
//
LOCUS       DAZKAS010000330          802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000330 DAZKAS010000000
VERSION     DAZKAS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.343"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>802
                     /locus_tag="ACG8H7_002993"
     CDS             <1..>802
                     /locus_tag="ACG8H7_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985005.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNG"
BASE COUNT          296 a          154 c          160 g          192 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aa
//
LOCUS       DAZKAS010000331          801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000331 DAZKAS010000000
VERSION     DAZKAS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.344"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>801
                     /locus_tag="ACG8H7_002994"
     CDS             <1..>801
                     /locus_tag="ACG8H7_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFP8985006.1"
                     /translation="MEILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGF
                     NSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQK
                     GITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIP
                     LKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVV
                     TDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKL"
BASE COUNT          274 a          117 c          148 g          262 t
ORIGIN      
        1 atggaaatac taatcaataa aggtgatttg gatgatttgt ttcgtcgtca cctagaattg
       61 gctattaacg ctttattaca agctgaatta acggtatttc ttgactatga aaagtatgat
      121 agagctggat ttaattcagg taattcccgt aatgggaatt attcgcgctc ttttaaaaca
      181 gagtacggtg aattgaattt agtaatacct agagatagga atggagactt ttctcaacaa
      241 actttacctg cttataaaag aaccaatgac tctttagaaa ccactattat tcagctagtt
      301 caaaaaggaa ttactatgtc cgaaatctct gatctaatcg aaaaaatgta tggtcattat
      361 tacaaaccac aaactatttc aaatatgagt aaaattgtgt ctgaagatgt ttcggcattt
      421 aaagaaagaa ctttagaagc taaatactca gtcattttta tggatgctac tcatattccc
      481 ttaaaaagac aaaccgtatc aaaagaagct gtctatatag taattggtat caggttggat
      541 ggaactaaag aggttctagg atttactata gctccaactg aatctgctta tgtttggaaa
      601 gagattcttc aagatctaaa agaccgtggt ttagaagagg ttttgttagt cgtaacagat
      661 ggtttaagtg gtattgacga tagtattcat agtgtttatc caaatgctca atttcaacaa
      721 tgttgtgtcc atatatctag aaatatcgct cataaggttc gtgttagtga tcgacaagaa
      781 atctgtagcg atttcaaatt a
//
LOCUS       DAZKAS010000332          774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000332 DAZKAS010000000
VERSION     DAZKAS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.345"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG8H7_002995"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG8H7_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP8985007.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          204 a          173 c          123 g          274 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attc
//
LOCUS       DAZKAS010000333          762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000333 DAZKAS010000000
VERSION     DAZKAS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.346"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..563
                     /locus_tag="ACG8H7_002996"
     CDS             75..563
                     /locus_tag="ACG8H7_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFP8985008.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            550..>762
                     /locus_tag="ACG8H7_002997"
     CDS             550..>762
                     /locus_tag="ACG8H7_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985009.1"
                     /translation="MEKIKQTFPLINKYWNKKKIFIFFITFLLSSLFSLIFPYLFSNV
                     IDLYLENELSQNIVRIVTILIFQLFLV"
BASE COUNT          292 a           64 c          132 g          274 t
ORIGIN      
        1 tattgtatag tttttgtttt tatgtgaaaa catgagcaat agaattgtac tagtaggata
       61 aggatggatg gaaaatgaaa aaaactattt ttgttttagc gatttttata tgttttgggc
      121 tacttttggt aggctacttg ttgtttaatt ctaatgaaat gagaactatt tctacgaatg
      181 acttgttaaa aaaaagtgaa ttaaaggata aaaaaaaaga atctcttgcc tttataatgt
      241 ttactcaaga agagtgtgag tattgtatag caataaatcc aatcatagat agtgtaaata
      301 aggaaacttt gaataagata aagaaatatg atatatctgt agattttcaa tctaaacaaa
      361 agtgtaaaat taagtttgtt ccgactattg ttgcatatag taagaataaa gaagtgggga
      421 gaattgacaa tgaattagat aatttgtatc gaaaaaagca agatggttca attgtaatag
      481 acaagaaaaa aacaaagaca ttgcttatag attggatgaa aaaaatggat aaacaaggag
      541 aatatttgta tggagaagat taaacagaca tttccgttaa ttaataaata ttggaataag
      601 aaaaaaattt ttatattttt tattacgttt ctgctgtcat ctttattttc tctaattttc
      661 ccgtatctat ttagtaatgt tattgattta tatttagaga atgagttatc acagaatatt
      721 gtgcgaatag taacgattct tatatttcag ttatttttag tc
//
LOCUS       DAZKAS010000334          752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000334 DAZKAS010000000
VERSION     DAZKAS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.347"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..>752)
                     /locus_tag="ACG8H7_002998"
     CDS             complement(91..>752)
                     /locus_tag="ACG8H7_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFP8985010.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          204 a          176 c          144 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gc
//
LOCUS       DAZKAS010000335          751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000335 DAZKAS010000000
VERSION     DAZKAS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.348"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>751
                     /locus_tag="ACG8H7_002999"
     rRNA            <1..>751
                     /locus_tag="ACG8H7_002999"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          231 a          148 c          220 g          152 t
ORIGIN      
        1 ctttaatagg atgttacgat tgtgtgaata catagcacat tcgaggtaga cgcagagaac
       61 tgaaacatct aagtacctgc aggaagagaa agaaaattcg attccctgag tagcggcgag
      121 cgaaacggga aaagcccaaa ccagcaagct tgcttgttgg ggttgtagga ctccaatatg
      181 gtagttcttt cagatagtcg aatgacttgg aaaagtcagt caaagagggt aaaaaccccg
      241 tagacgaaat gtggaagaca cctaggagga tcctgagtac ggcggaacac gagaaattcc
      301 gtcggaatcc gggaggacca tctcccaagg ctaaatactc cctagtgacc gatagtgaac
      361 cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg agtgaaatag aacctgaaac
      421 cgtgtgccta caacaagtca aagcccgtta atgggtgatg gcgtgccttt tgtagaatga
      481 accggcgagt tacgattgca tgcgaggtta agttgaagag acggagccgc agcgaaagcg
      541 agtctgaata gggcgtttga gtatgtagtc gtagacccga aaccatgtga tctacccatg
      601 tccaggttga aggtgcggta aaacgcactg gaggaccgaa cccacgtacg ttgaaaagtg
      661 cggggatgag gtgtgggtag cggagaaatt ccaaacgaac ttggagatag ctggttctct
      721 ccgaaatagc tttagggcta gcctcggaat t
//
LOCUS       DAZKAS010000336          748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000336 DAZKAS010000000
VERSION     DAZKAS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.349"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>748
                     /locus_tag="ACG8H7_003000"
     rRNA            <1..>748
                     /locus_tag="ACG8H7_003000"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          171 c          232 g          161 t
ORIGIN      
        1 tctaaccaga aagccacggc taactacgtg ccagcagccg cggtaatacg taggtggcaa
       61 gcgttgtccg gatttattgg gcgtaaagcg agcgcaggcg gtttcttaag tctgatgtga
      121 aagcccccgg ctcaaccggg gagggtcatt ggaaactggg agacttgagt gcagaagagg
      181 agagtggaat tccatgtgta gcggtgaaat gcgtagatat atggaggaac accagtggcg
      241 aaggcggctc tctggtctgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga
      301 ttagataccc tggtagtcca cgccgtaaac gatgagtgct aagtgttgga gggtttccgc
      361 ccttcagtgc tgcagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg
      421 aaactcaaag gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag
      481 caacgcgaag aaccttacca ggtcttgaca tcctttgacc actctagaga tagagcttcc
      541 ccttcggggg caaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt
      601 gggttaagtc ccgcaacgag cgcaaccctt attgttagtt gccatcattc agttgggcac
      661 tctagcaaga ctgccggtga caaaccggag gaaggtgggg atgacgtcaa atcatcatgc
      721 cccttatgac ctgggctaca cacgtgct
//
LOCUS       DAZKAS010000337          713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000337 DAZKAS010000000
VERSION     DAZKAS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.350"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..448)
                     /locus_tag="ACG8H7_003001"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="ACG8H7_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT          217 a          141 c          105 g          250 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tatatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaatcac acctataaat ttattactat ata
//
LOCUS       DAZKAS010000338          712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000338 DAZKAS010000000
VERSION     DAZKAS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.351"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8H7_003002"
     CDS             complement(<1..446)
                     /locus_tag="ACG8H7_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985011.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAZKAS010000339          703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000339 DAZKAS010000000
VERSION     DAZKAS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.352"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>703)
                     /locus_tag="ACG8H7_003003"
     CDS             complement(<1..>703)
                     /locus_tag="ACG8H7_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985012.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNG"
BASE COUNT          173 a          146 c          131 g          253 t
ORIGIN      
        1 tcccattcaa cgcaaaaaaa taaatagtga cggtttttcc ttcacgataa agctttgctg
       61 atccggtatt cccgtttgta actgttaagg ttacgacttc atatttgttg tcgtctgatg
      121 tcaatacaga ttttcctttt gattgaatac catcttcgaa atttttcaat ccttcgatgg
      181 attgcggttc agtcaaacta accgtatcat tcaagccttt ttcagtatat tcaggcgtga
      241 tgtcccagct gtagtcgttc ggattgttgc tatctttcaa tccttcacca aagtatttaa
      301 actgactaat attcggggtt cgggtgtcgc ctttttcaat tttgagccag tcaatttggc
      361 atgcgccggc cgtttcttta ggcaactgaa aaatgcgaag gtctttaggc aaacccggtt
      421 caagtttcgt tggtgtgaat gttagagacc atacgtctgt caatccctca actggtttta
      481 gttctccaaa attaacgttc caataattat atgctacaaa ggtttgactt gcgggttttg
      541 ttcctttaag cgtgatagta tacgtttgac ctattataag ctcttcttcc gtgttacctt
      601 tatatatttc gtacgcgcta gatttaattg ggaacgtcgt acctttattt gcaatattct
      661 cacccaacga tgttttacct acccagtaag ggtcatctag taa
//
LOCUS       DAZKAS010000340          672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000340 DAZKAS010000000
VERSION     DAZKAS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.353"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..536
                     /gene="spxA"
                     /locus_tag="ACG8H7_003004"
     CDS             138..536
                     /gene="spxA"
                     /locus_tag="ACG8H7_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFP8985013.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          233 a          101 c          132 g          206 t
ORIGIN      
        1 ctttcatttt tttaaaaaaa ttatgaaaaa aaggtagcta tttaaaagtt gattatgtat
       61 aatagaaaca gatgggagat tattatgatc ttctgttaca aaaaagtgat tcttatcacg
      121 aaaaggagtg gtgcacgttg ttaacattat atacttcccc aagttgtact tcctgcagaa
      181 aggctcgtgc gtggttgcaa gaacatcaga ttccatttgt tgaacgtaat attttttcag
      241 aaccattgaa cagttctgaa ttaaaagcga ttttacaaat gacagaagac ggaacagaag
      301 aaatcatctc gacacgttcc aaagtattcc aaaaactgaa catggattta gatgatctgc
      361 cgttacaaga attattggaa cttgtgcaaa acaatcctgg attgcttcgc cgtccaatca
      421 tgattgatga taaacgtctg caagttggtt ttaatgaaga tgaaattcgt cggttcctac
      481 cgcgcgatgt acgacaatta gaattacgtc aagcacaatt gatggctgga ttatagaagg
      541 ataatacgaa acttggaaaa gactggctct ttgtcaaatg atgttggtaa agagaaatct
      601 tagaataatt tggacagaac agaaagaaga aaacctcttt ctgttctgtt tatttggtta
      661 aaaatgtttt gc
//
LOCUS       DAZKAS010000341          661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000341 DAZKAS010000000
VERSION     DAZKAS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.354"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..>661)
                     /locus_tag="ACG8H7_003005"
     CDS             complement(159..>661)
                     /locus_tag="ACG8H7_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFP8985014.1"
                     /translation="GIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGVLVILEGGMII
                     DTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVMAPLGDLMNVS
                     RDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFAILTLVSAVLL
                     YIATLF"
BASE COUNT          241 a          132 c          117 g          171 t
ORIGIN      
        1 atgcggagca cctccccaaa gttcaacaga agaaatacca attttctgtt gataagcgaa
       61 aaattctttg agagaataaa ataaatagtg gatattcatt cctgctaatt gatttatttt
      121 aatcataact attgcctcct attaatggat gattaagact aaaagagtgt agcaatgtat
      181 aacaaaacag cagaaacaag ggttaagatg gcaaatattt tgaagccaac cttaaaccat
      241 ttagagaaat taatctctgc gatggctaat cctgccagta atacaccact agttggggag
      301 ataatattca ttaaaccatt tccaaactgg ttacttagaa caacaacatc acgacttaca
      361 ttcattagat cacccaatgg tgccataacc ggcatcgtta atgccgcagc tcctgaggag
      421 gatggaacaa aaaaggaaac aattacttga gaaatgagta gaatcgtagt gaacaggaat
      481 tttggaagac cacctaaaag gttagccaag ctgtttagaa tagtgtcaat aatcattcca
      541 ccttccaaga ttaccaagac tcctctagct agcccaatga ttacagcagc ataggcgaaa
      601 tctgaacagc cttcgacaaa agcttcagca atttcctttt gagaaaatct agcgatgccc
      661 c
//
LOCUS       DAZKAS010000342          637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000342 DAZKAS010000000
VERSION     DAZKAS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.355"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>637
                     /locus_tag="ACG8H7_003006"
     CDS             51..>637
                     /locus_tag="ACG8H7_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFP8985015.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEA"
BASE COUNT          231 a          106 c          126 g          174 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccat
//
LOCUS       DAZKAS010000343          587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000343 DAZKAS010000000
VERSION     DAZKAS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.356"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..326)
                     /locus_tag="ACG8H7_003007"
     CDS             complement(<1..326)
                     /locus_tag="ACG8H7_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985016.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGT"
BASE COUNT          187 a           95 c           84 g          221 t
ORIGIN      
        1 gttccaataa ggatatcttt tacgcgttgg acctgttcag gcgtcagtgt ttctccgcca
       61 taagacatat tgacatttga atctagtaat ttatcaagtt caactacttc atcttttgtt
      121 gcccagtcag gaacttggtt tctaccgagt aaataatcag tagttacatt aaaataatct
      181 gctacaagtt gtagtctttt agtactagga gttctatttt tccattgata aatcgtgttt
      241 ttaggtatat ttaattcttc ttccaattga gttatactta tgcctctttt atgagctaat
      301 tcctttattc tatctagtaa attcattttc gcacctcaca agctacgaaa acactaataa
      361 aaaaattagt taaaggtgtt gacaactaat aaaagtgtta gtatactgtt ttcgtaagct
      421 aaaatattag ctaaaaagac ttgataacta ataaacactt cacggtcggc aaactctgaa
      481 atgtaaatta ctaggcattt ctgtgtctta tttagctatg tctatatact aataaaaata
      541 ttagttattg tcaacgaata ttagctaatt ttttagctta cagatta
//
LOCUS       DAZKAS010000344          576 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000344 DAZKAS010000000
VERSION     DAZKAS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.357"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>576
                     /locus_tag="ACG8H7_003008"
     CDS             <1..>576
                     /locus_tag="ACG8H7_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985017.1"
                     /translation="KSTTKFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHY
                     FDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDN
                     PENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGE
                     DKEKRIDNIINYINSLSEPLKCRGLDFIGTLG"
BASE COUNT          236 a           60 c          106 g          174 t
ORIGIN      
        1 aaaagtacga ctaaatttgc aaactcattc tggtttttaa aaaatattaa taaaacagtg
       61 gaaaagaatg gagtttataa aggtgatttt cattttcaaa atgctgagac tattgaacga
      121 gaacatcatt atttcgattt attagtgaat acagtggtag aattagcgaa atgggcaaag
      181 tcaaacgaaa cttatagtca agtacttaaa agaattatga gtcaagaaac ttcatttatc
      241 tatgagttaa gcaagtcaaa tttgaaatat acttataatg aagatgaact tgaaatttta
      301 gataatccag aaaattcaca atggattaaa gagttgaaga gagaagactt tattgagaca
      361 ctaagaattc taaaatcgtt atttgatttg aaattagcca aagctaccat catgggaatt
      421 gaaagagatt catattattg ggaggaaatt ggactatatg ctccccagat tgaaggtgaa
      481 gataaagaga aaaggataga caacattatt aactatatta atagtttaag tgaaccttta
      541 aaatgtagag gacttgattt tataggaaca ttagga
//
LOCUS       DAZKAS010000345          553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000345 DAZKAS010000000
VERSION     DAZKAS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.358"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..>553
                     /locus_tag="ACG8H7_003009"
     CDS             57..>553
                     /locus_tag="ACG8H7_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFP8985018.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          202 a          103 c           90 g          158 t
ORIGIN      
        1 tcccttctgt agaataaagt taacgacaac caattcacag aaaagaggac ttccctatga
       61 atgattttac tacagaaatt gtgcaaactc tagtcactaa aggcgattta aatgaattat
      121 tccgttcgca cttagaaaaa gcgataaaca cactcctacg gactgaatta acggcttttt
      181 tagattacga aaaatatgat cgcactggtt ttaattcagg taattcgaga aacggttctt
      241 actttcgatc aatcaaaacc gaatatggtg aattaacatt ggaaatacct agagatcgta
      301 atggtgagtt taaacaacaa actttaccag cctacaaaag aacaaacgat acattggaaa
      361 ccactattat ccatttattc gaaaaaggtg ttacgatgtc tgaaattgct gatttgatcg
      421 aaaaaatgta cggtcatcac tatactccac aaaccatgtc caacatgact aaagttctga
      481 ctgaagaagt aaatgccttt aaatccagag ccttaaatga taagtatgtc gctattttta
      541 tggacgctac tta
//
LOCUS       DAZKAS010000346          544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000346 DAZKAS010000000
VERSION     DAZKAS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.359"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..360
                     /locus_tag="ACG8H7_003010"
     CDS             <1..360
                     /locus_tag="ACG8H7_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFP8985019.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          202 a           89 c          116 g          137 t
ORIGIN      
        1 cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc
       61 tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt
      121 ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat tctagaagct
      181 ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt taatgagcag
      241 acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt aagctatgag
      301 caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga taagaagtga
      361 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggaa
      421 aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
      481 acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg
      541 taca
//
LOCUS       DAZKAS010000347          542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000347 DAZKAS010000000
VERSION     DAZKAS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.360"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..75
                     /locus_tag="ACG8H7_003011"
     CDS             <1..75
                     /locus_tag="ACG8H7_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFP8985020.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            249..>542
                     /locus_tag="ACG8H7_003012"
     CDS             249..>542
                     /locus_tag="ACG8H7_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985021.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
BASE COUNT          213 a           94 c          105 g          130 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatc gaaaagcaaa aagcatggca
      181 acaaataaaa aagtgactca gccggcaagc atagagtcac aaaacatatc taaggagaat
      241 tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat tctgaatggg
      301 gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacgaacg
      361 aagaactatc atgtgatcaa gatgacttgg gaaattttgt gagtatttgg gatcatgttt
      421 accttatcca tctatcaaaa cattcaaata aacctgaata catctatgtc atcgaagatg
      481 gcttgattga tgcgctagaa gagtacaaca gagataactt gattgatatc tcttattacg
      541 ga
//
LOCUS       DAZKAS010000348          540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000348 DAZKAS010000000
VERSION     DAZKAS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.361"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACG8H7_003013"
     CDS             143..400
                     /locus_tag="ACG8H7_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985022.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          229 a           66 c           77 g          168 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
//
LOCUS       DAZKAS010000349          534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000349 DAZKAS010000000
VERSION     DAZKAS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.362"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          185 a           58 c           92 g          199 t
ORIGIN      
        1 tgacataatt gcattgtatc cgctttcttt gtgtttgtca ataggaaaaa tttatttctc
       61 taaaaaatat aaataatctg cagtgaaact tgagtacata tactaatttc gaaaaatgcc
      121 tatgatttaa ataattatgg tgctgggatg aaggtttata agtttgtctt gatttgttta
      181 gaagaaatcg tttttgcaca cagttgaatt aggaaactac tggagtggaa aataaagtga
      241 tttgacgggt agatgggtgc acttaaattt gaaaatggct taaaatgctt tattttgaat
      301 tgcagaattt cttttagtac gcaatttatg gtcttaaaac tgattaaaaa gtgatacatt
      361 gattttaata caatttatga tacaaaaaat gacctccttt cttaaataaa agaggtcatt
      421 ttgttttgtc tcataatcga ttgtttttga tacagtagtt atattcagaa aaaataaaga
      481 ctcttctgag tttataatcc tttggattat atacggatac agatgtagac ataa
//
LOCUS       DAZKAS010000350          532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000350 DAZKAS010000000
VERSION     DAZKAS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.363"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..502
                     /locus_tag="ACG8H7_003014"
     CDS             146..502
                     /locus_tag="ACG8H7_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFP8985023.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          206 a           80 c          104 g          142 t
ORIGIN      
        1 ttgagaaaaa cttcaagata gtccttaact ttcttttgaa gcaataagaa caaaatgagg
       61 aaaagtttaa aattcagctg ttgccaacaa ctttcaatag acgaaatctt ctatttcgtt
      121 aataataagg aaagaggtga agtacatgaa aaaattaatt ggcgtacttg tcgttttagg
      181 catatttctc ggcggaagtt ttgcagctta tcattatttt tacggcggcg aagcttatta
      241 tacaaaaatc acgacgaatg gcgaaatgtc agctacccaa gcagataacg gagagaaatt
      301 cacgacttat acctacaagc aagcggctta taataaagat ggtgaaaaaa aacaggtgac
      361 gcttcgcgaa gaaagagaac atccattaaa gatgaatgcg tatcttaaac tcaaagtcaa
      421 tcctcgtaaa ggtgtattaa gctgggaaga aataaaggct tctgaagtgc ctaaaaatgc
      481 ggcagaaaaa atcgataatt agtaaatggt cttaaaattt tattagataa aa
//
LOCUS       DAZKAS010000351          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000351 DAZKAS010000000
VERSION     DAZKAS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.364"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG8H7_003015"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG8H7_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP8985024.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAZKAS010000352          520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000352 DAZKAS010000000
VERSION     DAZKAS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.365"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>520
                     /locus_tag="ACG8H7_003016"
     CDS             <1..>520
                     /locus_tag="ACG8H7_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8985025.1"
                     /translation="LKTPLASLKIILENMKYNIGKYKERDIYINECIEIVDSLTKNIS
                     QILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTAL
                     KIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKEN
                     SNGLGLYIVSNIL"
BASE COUNT          230 a           36 c           77 g          177 t
ORIGIN      
        1 acttaaaacc cctcttgcta gtcttaaaat aatactggaa aacatgaaat acaatattgg
       61 aaaatataaa gaaagagata tttatattaa tgaatgtatt gaaatcgttg atagtttaac
      121 aaaaaatatt tctcaaatat tatcagttca ttctatagaa aatttgaata atgatgaaga
      181 atatttgaaa ataaatgata cattagaaga catattaaaa aagtatgaaa tattagcaaa
      241 tcaaaagaaa ataactgtca ataattatat attagacgag aatgtttata taggaaaaac
      301 agctttaaag attattctat ctaatttgat tagtaatgcg gtaaaatata ctgatgtaaa
      361 tggggtaatt aatattggga tagttaatga ttggttatat atagagaatt catacggtaa
      421 caataaaatt tcgaatatgg ataaaatatt tgatgttaaa tttgatttaa ataaggaaaa
      481 tagtaatgga ttaggtttat atattgtaag taatattctg
//
LOCUS       DAZKAS010000353          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000353 DAZKAS010000000
VERSION     DAZKAS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.366"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8H7_003017"
     CDS             complement(<1..132)
                     /locus_tag="ACG8H7_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP8985026.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKAS010000354          510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000354 DAZKAS010000000
VERSION     DAZKAS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.367"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..510
                     /locus_tag="ACG8H7_003018"
                     /pseudo
     CDS             1..510
                     /locus_tag="ACG8H7_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DAZKAS010000355          499 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000355 DAZKAS010000000
VERSION     DAZKAS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.368"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..477
                     /gene="scm"
                     /locus_tag="ACG8H7_003019"
     CDS             <1..477
                     /gene="scm"
                     /locus_tag="ACG8H7_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFP8985027.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          192 a          111 c           86 g          110 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagcgacagt acggatacat ctaccacaac aagtgatagc
       61 acggacacgt cagcttcaag tgacagtaca gatactacct ctaccacaag tgatagctca
      121 gattcatcta ccactacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat
      181 acatcctcta ccacaagtga tagctcagat tcatcaactt caagcgatag tacagacacg
      241 tcgtctacca caagcgatac acgtcatgca agtgtctcct tctcaaaaac aagttcggat
      301 aacaggacga actttaactt aactaataaa acaagcaatg acaatggaaa caaatcaaaa
      361 agagcacttc ctaaaactgg aagtcaatcg aataattgga taacactcgc aggcgttata
      421 ttacttgtaa tcggtttaag aatcactttt atgagttata agaaatcgaa aaggtaaaaa
      481 aatattcaag ataaaaaag
//
LOCUS       DAZKAS010000356          498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000356 DAZKAS010000000
VERSION     DAZKAS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.369"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>498
                     /locus_tag="ACG8H7_003020"
     CDS             <1..>498
                     /locus_tag="ACG8H7_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP8985028.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFT"
BASE COUNT          186 a           55 c           82 g          175 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgag
//
LOCUS       DAZKAS010000357          479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000357 DAZKAS010000000
VERSION     DAZKAS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.370"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8H7_003021"
     CDS             complement(<1..430)
                     /locus_tag="ACG8H7_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8985029.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          135 a           95 c           64 g          185 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcac
//
LOCUS       DAZKAS010000358          473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000358 DAZKAS010000000
VERSION     DAZKAS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.371"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..>473
                     /locus_tag="ACG8H7_003022"
     CDS             172..>473
                     /locus_tag="ACG8H7_003022"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFP8985030.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPT"
BASE COUNT          180 a           56 c           79 g          158 t
ORIGIN      
        1 ttcaattcat atttatttat aaatcaaaaa tacagagaat agtatataaa tgtcctttcc
       61 tattcttaaa atgatgtttg tacttactta aattaggtat aattagtata tgttttgtga
      121 gaaatttaga cttacaataa gcagtgaatt tataatcaac gtataggaga catggatgaa
      181 atgaaactaa ttggtataga tttagacggt actcttttga attctgacca gagcattagt
      241 caaaaaaatg caagaacaat gaaacattta ccggaagact gttttccatt catttgttct
      301 ggaagagaag tagaagacat taataatatt ctcaaaaaat ctggtttaag gataccagca
      361 gtagggttaa atggttctgt tggatttgat ggagaaaaaa agatatttga gtcttccttc
      421 gataaaaatg atgtaaaaaa aattgattct gtcctatcga aatttccaac caa
//
LOCUS       DAZKAS010000359          470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000359 DAZKAS010000000
VERSION     DAZKAS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.372"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACG8H7_003023"
     CDS             <1..107
                     /locus_tag="ACG8H7_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP8985031.1"
                     /translation="GISGAYLSDILNGNRDGKKAQEHIETVKKILGIQ"
     gene            120..458
                     /locus_tag="ACG8H7_003024"
     CDS             120..458
                     /locus_tag="ACG8H7_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFP8985032.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT          185 a           60 c           97 g          128 t
ORIGIN      
        1 ttggtatttc aggagcttat ttatcagata tcctaaacgg caatcgtgat ggtaaaaaag
       61 ctcaagaaca catagaaact gtaaaaaaaa tattaggtat tcaataggag gaacagctaa
      121 tgcaatatct agaagcgaaa atcccagttc cagaaggcta tgtaattatc tctcaagtgg
      181 attacgagga gttaaaaaaa gctgatgata ctggtagatg gatgacgttg ccagaagtgc
      241 tagaacggat taacagaaaa tatgattggt ttacttctag agttttaaag aacccaagat
      301 atagaaatat tatcgatatc gagaaaaaca aaaatggatt tgtctattat ccagttgaag
      361 gaagagacac atatctattt ttaagaagta aaacacttga atttttagaa acaaattttt
      421 cggaaatctt aaggaggcaa gcggatggga aaatttaata gagcgctagt
//
LOCUS       DAZKAS010000360          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000360 DAZKAS010000000
VERSION     DAZKAS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.373"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8H7_003025"
     tRNA            complement(20..91)
                     /locus_tag="ACG8H7_003025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8H7_003026"
     tRNA            complement(113..200)
                     /locus_tag="ACG8H7_003026"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8H7_003027"
     tRNA            complement(225..298)
                     /locus_tag="ACG8H7_003027"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8H7_003028"
     tRNA            complement(312..382)
                     /locus_tag="ACG8H7_003028"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8H7_003029"
     tRNA            complement(384..459)
                     /locus_tag="ACG8H7_003029"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKAS010000361          454 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000361 DAZKAS010000000
VERSION     DAZKAS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.374"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          147 a           88 c           52 g          167 t
ORIGIN      
        1 acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta
       61 gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt
      121 ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac
      181 tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact
      241 ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg
      301 tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca
      361 acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt
      421 atacactact attcagccca ttcaacgacc aatg
//
LOCUS       DAZKAS010000362          452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000362 DAZKAS010000000
VERSION     DAZKAS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.375"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACG8H7_003030"
     CDS             <1..>452
                     /locus_tag="ACG8H7_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985033.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAZKAS010000363          436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000363 DAZKAS010000000
VERSION     DAZKAS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.376"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          147 a           72 c           94 g          123 t
ORIGIN      
        1 gcaacaaaga aatttcatcg ggggaagagg tttggacaaa aatggaaatt tccgccaaaa
       61 gtaactgctc ctcaactgaa atcaattaaa gaactagaat ttgagaatgc actggtaatt
      121 tgtatgacat gtactgatga agtatttcga aaacattttt aaaggttgag atgtatatag
      181 atttaaccaa tcatttatct ctctcggaaa aggaatatta actaaccagc gggttatcaa
      241 aaataggtag gaaaactgta cttaatggct tatttcgcgc tgattatgag aatatcagtc
      301 tgtttgatta atttttaggg ttcaaaatac agagacaaac tccaggtcgg gtttcgccac
      361 aagcgaatta cccgatctgg agttttgtct attgcaggaa ggcgtagccg gactgcagga
      421 ataaagcccc cggttc
//
LOCUS       DAZKAS010000364          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000364 DAZKAS010000000
VERSION     DAZKAS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.377"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8H7_003031"
     CDS             complement(<1..339)
                     /locus_tag="ACG8H7_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985034.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZKAS010000365          409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000365 DAZKAS010000000
VERSION     DAZKAS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.378"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          148 a           71 c           64 g          126 t
ORIGIN      
        1 gtatagcaag caaaattctt ctccacttcc agaatttttt attttcattt ttagaaaagt
       61 gaaaaaactg acaacaaaag cttgtttctt tcactctatt aatatcttta gtgaaaaagc
      121 tcgttttata tcgtattctt ccagcgaatg tacttctcat aagtccaaaa aaagctgcga
      181 aaaagaaagt acctcggtac ctactttttc gtagctgcac ataacttgag caagtattaa
      241 tcgaagatac ttttgttgag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc
      301 ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca tatgaacgat
      361 tttactacag aaatcctaaa gactctagcg aacaaaggcg atttgaatg
//
LOCUS       DAZKAS010000366          404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000366 DAZKAS010000000
VERSION     DAZKAS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.379"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>404
                     /locus_tag="ACG8H7_003032"
     CDS             <1..>404
                     /locus_tag="ACG8H7_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFP8985035.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAZKAS010000367          401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000367 DAZKAS010000000
VERSION     DAZKAS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.380"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           98 c           76 g          115 t
ORIGIN      
        1 attataaata actcataaaa aatattttga ctttcaaaat cacttttgct accagaagtt
       61 gaggcgaagt gttttaagca tagttgtacc ctagtttgac cgttaaaaat aaataacgat
      121 cgctagagtg caactatgtt tgtttgccct accgtcagcg tcggcttggc ctctcacatc
      181 cattaccgtc gggattatct ccgttacata cccttacaaa atacgccctg taagcattgc
      241 atgaaaccac cgtgcatttg caccgtattt ccgccctagc cgttcaaatc ggtcgctacc
      301 accattcaga aacacgtgcg gtaatggaag gtttggagga agctatccag accactaacg
      361 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DAZKAS010000368          390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000368 DAZKAS010000000
VERSION     DAZKAS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.381"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..>390
                     /locus_tag="ACG8H7_003033"
     CDS             25..>390
                     /locus_tag="ACG8H7_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFP8985036.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHL"
BASE COUNT          159 a           45 c           57 g          129 t
ORIGIN      
        1 atttttgtta atgattttaa taaaatgatt ggagagaaaa tgatggatca aaaagcgttt
       61 atcgaatcta ttgattctac aatcgataga ttaaaaaaag aaattcgatt ttataataga
      121 gtaattggta tcggaaatat tataaaaata gtcttatcag ctagtatccc aatattaatt
      181 gatcaggctt ccgaacacag gtctttacta ttgattgttt caattgcttc agcaataatt
      241 acaattatac aaagtggtat gtctgcattt aattatcaag ataaagtaca gacttcgaca
      301 gagttattaa tgaaaattga aaaagaaaag ttgttatata taacgaaaac atccccatat
      361 aataaaacag atgaagaaaa tttccattta
//
LOCUS       DAZKAS010000369          378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000369 DAZKAS010000000
VERSION     DAZKAS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.382"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG8H7_003034"
     CDS             complement(<1..281)
                     /locus_tag="ACG8H7_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985037.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
BASE COUNT          117 a           73 c           60 g          128 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaattttt
//
LOCUS       DAZKAS010000370          352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000370 DAZKAS010000000
VERSION     DAZKAS010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.383"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>352)
                     /locus_tag="ACG8H7_003035"
     CDS             complement(<1..>352)
                     /locus_tag="ACG8H7_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8985038.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQ
                     RLAQRILDNELGCAN"
BASE COUNT          106 a           75 c           64 g          107 t
ORIGIN      
        1 cgttagcaca tccgagctca ttatcaagga ttcgctgcgc caatctctgg ttacggtaac
       61 caaccggtgt ctcgtagcca agtgtaccgt gcaaccgaag gtggttccac caattgacat
      121 agtcaaataa ctccaaatcc aattgttgta aggtttcaaa tgtgtattga tagacaaatt
      181 ctactttcaa cgacttataa gttgattcag ctacggcatt atcaaaagga cagcctttat
      241 gactcaatga tcgattgatg tcaaaagttg ttaataattc atcaatagct tggttatcaa
      301 actcttttcc acgatcagta tgaaaaatct caacctctgt cagaggttgt tt
//
LOCUS       DAZKAS010000371          349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000371 DAZKAS010000000
VERSION     DAZKAS010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.384"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          144 a           34 c           58 g          113 t
ORIGIN      
        1 ataataaata aaaataggga attttctatt gcaattttat aaagagattg ctatattatt
       61 atttgttcta aatgattcct ttaaggtgtc actaaaaatt ataaaaaata gtgttgacgc
      121 acttttgatg tcatgttata ataacgaagt cgtttggatg agatagctaa taaagcttga
      181 ttttatcgaa aaaaacttcg aaaaagttat tgacaaataa caataaagat ggtatgataa
      241 tcaagtcgct gctaatgagt ggcaaagacg tttagaaaaa ataaacaaag aagttgttga
      301 cattctcttc tgaatctgat aagattaaag agttgttaaa acaaaaaga
//
LOCUS       DAZKAS010000372          329 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000372 DAZKAS010000000
VERSION     DAZKAS010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.385"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>329
                     /locus_tag="ACG8H7_003036"
     CDS             <1..>329
                     /locus_tag="ACG8H7_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP8985039.1"
                     /translation="YVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIVFT
                     MLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIWGI
                     ISKGWYM"
BASE COUNT           91 a           54 c           72 g          112 t
ORIGIN      
        1 gatatgttgg gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg
       61 tttacggaaa tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt
      121 caattgtttt caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg
      181 tttatgagag tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt
      241 tttcaacaca agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt
      301 ggggtatcat ttccaaggga tggtatatg
//
LOCUS       DAZKAS010000373          328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000373 DAZKAS010000000
VERSION     DAZKAS010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.386"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>328
                     /locus_tag="ACG8H7_003037"
     CDS             52..>328
                     /locus_tag="ACG8H7_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP8985040.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          104 a           60 c           63 g          101 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DAZKAS010000374          326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000374 DAZKAS010000000
VERSION     DAZKAS010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.387"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>326
                     /locus_tag="ACG8H7_003038"
     CDS             <1..>326
                     /locus_tag="ACG8H7_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8985041.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGL
                     PKEMEF"
BASE COUNT          115 a           67 c           59 g           85 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaagat
      301 ggactaccaa aagaaatgga attcaa
//
LOCUS       DAZKAS010000375          322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000375 DAZKAS010000000
VERSION     DAZKAS010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.388"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..>322
                     /locus_tag="ACG8H7_003039"
     CDS             27..>322
                     /locus_tag="ACG8H7_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985042.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIY"
BASE COUNT          112 a           47 c           40 g          123 t
ORIGIN      
        1 ttattacttt taaataggag aaaaaaatga aaaaaaacaa aaaccaaata atagatatca
       61 tttttatgtt atttctaagt tttgcttacg tcatccctct attcatgtct aagggaatct
      121 atcatagtgt caatcaggat acttactttc atttatctcg aatcatagga ctagataatg
      181 tatggagttc tccagtaaac ttcaataact tcgatcatca tggaacaatg atgaatattt
      241 tttatccatg gcttactctt tatccagctt ttttatttta taaaatgatg ggaaatcttg
      301 ttttaagtta taatatctac ta
//
LOCUS       DAZKAS010000376          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000376 DAZKAS010000000
VERSION     DAZKAS010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.389"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8H7_003040"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8H7_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8985043.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZKAS010000377          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000377 DAZKAS010000000
VERSION     DAZKAS010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.390"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8H7_003041"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8H7_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFP8985044.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           97 a           45 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatat
//
LOCUS       DAZKAS010000378          308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000378 DAZKAS010000000
VERSION     DAZKAS010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.391"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACG8H7_003042"
     CDS             complement(76..>308)
                     /locus_tag="ACG8H7_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8985045.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAZKAS010000379          305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000379 DAZKAS010000000
VERSION     DAZKAS010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.392"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>305
                     /locus_tag="ACG8H7_003043"
     CDS             <1..>305
                     /locus_tag="ACG8H7_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFP8985046.1"
                     /translation="YTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYF
                     FIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMI"
BASE COUNT          103 a           57 c           48 g           97 t
ORIGIN      
        1 gctatactag ccaaagagcc acgtatagag cggtttgttc cgtcttattt cctaatggtg
       61 actttcctag taggagtcga ttcactcgct taagttcaaa cttagcttac actatcaaga
      121 ttattcgata ctttttcatc aagaagctca ccaaaggtga actagtcgga attatagata
      181 gttttcctag tccattatgt aaaccagtta gaaatagaca agcaaaacta ttgaatcaaa
      241 tagccaaagt tggctataac tcaacaaaaa aatcttattt ttatggtctt aaaattcata
      301 tgatc
//
LOCUS       DAZKAS010000380          302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000380 DAZKAS010000000
VERSION     DAZKAS010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.393"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>302
                     /locus_tag="ACG8H7_003044"
     CDS             <1..>302
                     /locus_tag="ACG8H7_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8985047.1"
                     /translation="GDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQW
                     FSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRR"
BASE COUNT           94 a           62 c           52 g           94 t
ORIGIN      
        1 ggtgatacga tcgttggcat tcatcataaa agtgccgtca ttactttagt tgaaagatta
       61 tctaaagtca ttatcacgat taaacccaac ggccgtaagg cattagatat tgaaactgcc
      121 cttaatcaat ggttttctcg cttccctaaa aacttcttta aatctattac gtttgactgt
      181 ggaaaagaat tttctaactg gaaagccatt agtaaccaac atgatattga tatatatttt
      241 gcggaccctg gaacaccttc tcaacgccca ttaaacgaga attctaacgg gattctgcgt
      301 cg
//
LOCUS       DAZKAS010000381          302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000381 DAZKAS010000000
VERSION     DAZKAS010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.394"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..>302)
                     /locus_tag="ACG8H7_003045"
     CDS             complement(54..>302)
                     /locus_tag="ACG8H7_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985048.1"
                     /translation="TIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIY
                     MFILYLTDNTLTYPDTQMIFLLVPMIVSWVDNQQRISE"
BASE COUNT          120 a           50 c           27 g          105 t
ORIGIN      
        1 tattaacttt tacaactaat tataaattca taattaaaat atactttagc tatctattcc
       61 gatattcgtt gttgattatc aacccacgaa actatcatag gtactaagag aaaaatcatt
      121 tgagtgtctg gataagtgag cgtattatcc gtcaaatata atataaacat atatatagtt
      181 aataataata cacactcccc tgttttcaat ccaaattttt tagttagtct tatcggtttc
      241 acaaaaaata accaaaacat ccatataata aaaactatac atccaacttc tatataaata
      301 gt
//
LOCUS       DAZKAS010000382          287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000382 DAZKAS010000000
VERSION     DAZKAS010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.395"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..287
                     /locus_tag="ACG8H7_003046"
                     /pseudo
     CDS             1..287
                     /locus_tag="ACG8H7_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          112 a           58 c           56 g           61 t
ORIGIN      
        1 aaacaacaaa tcgtcgatct ctatctcgct ggtaagcctc gcgcagaaat tattcgagag
       61 tatgagctta cgccttcttc tttcgataaa tggatgaagc aagcacaatc aacgggctca
      121 ttcaaagaaa gagacaactt aacaccagaa caagcagaat tgatcgcact aagaaagaaa
      181 aataagcaac tcgaaatgga gaatgatatt ttaaagcaag cggcgctgat attcggacga
      241 aaagacaagt aattgatgcc aacaagcata aatattccat atcagcg
//
LOCUS       DAZKAS010000383          282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000383 DAZKAS010000000
VERSION     DAZKAS010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.396"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG8H7_003047"
     CDS             <1..>282
                     /locus_tag="ACG8H7_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFP8985049.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZKAS010000384          270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000384 DAZKAS010000000
VERSION     DAZKAS010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.397"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACG8H7_003048"
     CDS             <1..>270
                     /locus_tag="ACG8H7_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP8985050.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAZKAS010000385          263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.398, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000385 DAZKAS010000000
VERSION     DAZKAS010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.398"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>263
                     /locus_tag="ACG8H7_003049"
     CDS             <1..>263
                     /locus_tag="ACG8H7_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFP8985051.1"
                     /translation="TTTEFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADA
                     NANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT           96 a           49 c           57 g           61 t
ORIGIN      
        1 accaccacag agtttacaaa atttgcttcg gaatttaata ctgggatttc ttccgggaac
       61 gccaaagcga atagcttgat cgttggaacg ggaagtgcca aagaattaag tgaaaccctg
      121 ttggcagatg ccaatgcaaa tgttgtaatc attacaaccc gtcaaaaact agatgcggcg
      181 ttgaaatatg caaagaaaca agaagagaaa aaaggaacca atcgcttcca aaaattaatg
      241 ggccaacata tcgtgtttgt agt
//
LOCUS       DAZKAS010000386          263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000386 DAZKAS010000000
VERSION     DAZKAS010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.399"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>263
                     /locus_tag="ACG8H7_003050"
     CDS             <1..>263
                     /locus_tag="ACG8H7_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFP8985052.1"
                     /translation="TTTEFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADA
                     NANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT           90 a           45 c           64 g           64 t
ORIGIN      
        1 accaccacag agtttacgaa atttgcttcg gagtttaata ctggggtttc ttctggtaat
       61 gccaaagcga atagcttgat cgtgggaaca ggaagtgcca aagagttaag tgaaactctg
      121 ttggcagatg ccaatgcgaa tgttgtgatc attacaaccc gtcaaaaact agatgcggcg
      181 ttgaaatatg caaagaaaca ggaagagaaa aaaggaacca atcgcttcca aaaattaatg
      241 gggcaacata tcgtgtttgt agt
//
LOCUS       DAZKAS010000387          255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000387 DAZKAS010000000
VERSION     DAZKAS010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.400"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           46 c           39 g           91 t
ORIGIN      
        1 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
       61 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct cctttcacaa
      121 gtatggtgat aaaattcgtt ccgggctacc tttttaggta gcctacttta atctttatac
      181 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg
      241 tccggcacgt cagtc
//
LOCUS       DAZKAS010000388          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.401, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000388 DAZKAS010000000
VERSION     DAZKAS010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.401"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACG8H7_003051"
     CDS             complement(32..>252)
                     /locus_tag="ACG8H7_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFP8985053.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAZKAS010000389          238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000389 DAZKAS010000000
VERSION     DAZKAS010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.403"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           88 a           27 c           41 g           82 t
ORIGIN      
        1 aaggatgttt cacatagtta tatttctgta tatttgaaaa gttttacttt gatttttaaa
       61 tagaaagaca tttgggttat attgtgagat aataataaag aagagtttaa agcgttcccc
      121 aaaaaccact gccccataag tgtgttacgc tttaaactct tttatatttg aagccattaa
      181 aaagcatacc atatttttga aaaaaagtga gaaaaaaggc ttataattgg agtggtag
//
LOCUS       DAZKAS010000390          227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000390 DAZKAS010000000
VERSION     DAZKAS010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.404"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="ACG8H7_003052"
     rRNA            complement(<1..>227)
                     /locus_tag="ACG8H7_003052"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAZKAS010000391          226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000391 DAZKAS010000000
VERSION     DAZKAS010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.405"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>226
                     /locus_tag="ACG8H7_003053"
     CDS             <1..>226
                     /locus_tag="ACG8H7_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP8985054.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFKL"
BASE COUNT           71 a           48 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttac
//
LOCUS       DAZKAS010000392          217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000392 DAZKAS010000000
VERSION     DAZKAS010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.406"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..>217
                     /locus_tag="ACG8H7_003054"
     CDS             57..>217
                     /locus_tag="ACG8H7_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFP8985055.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT           81 a           40 c           33 g           63 t
ORIGIN      
        1 catttgtgct acactcaaaa tagttccgac caaagaatta taaggagtga gtgcaaataa
       61 cctataccta tcttacaacg gatgaacttg taataataga gtcttatttg aaaattaacc
      121 aatctgttgc taaaactgcg cattgcttga atcgttcaag tcaaacgatc cataaagtat
      181 atctgttctt caagcaaaga aaatcagcct tagaata
//
LOCUS       DAZKAS010000393          212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000393 DAZKAS010000000
VERSION     DAZKAS010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.407"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           80 a           27 c           46 g           59 t
ORIGIN      
        1 aatcgatcag agtgaagcag acatacaaat tgaaagtcta caagaactgg aacaaattgt
       61 tttagaaaaa ggttagtgaa ttaatggaaa taaggctata tatctgtgct agctattttg
      121 actaaccaaa aaaacttgct ataattcagg tgtagtaaaa ggtgctggta acacctttta
      181 cgaaaatgct tcggcagtga taatgggatt gg
//
LOCUS       DAZKAS010000394          206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000394 DAZKAS010000000
VERSION     DAZKAS010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.408"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>206)
                     /locus_tag="ACG8H7_003055"
     CDS             complement(<1..>206)
                     /locus_tag="ACG8H7_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP8985056.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DAZKAS010000395          203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000395 DAZKAS010000000
VERSION     DAZKAS010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.409"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>203)
                     /locus_tag="ACG8H7_003056"
     rRNA            complement(<1..>203)
                     /locus_tag="ACG8H7_003056"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           39 a           63 c           47 g           54 t
ORIGIN      
        1 taacaacaga gttttacgat ccgaaaacct tcttcactca cgcggcgttg ctcggtcaga
       61 ctttcgtcca ttgccgaaga ttccctactg ctgcctcccg taggagtttg ggccgtgtct
      121 cagtcccaat gtggccgatc accctctcag gtcggctatg catcgtggcc ttggtgagcc
      181 gttacctcac caactagcta atg
//
LOCUS       DAZKAS010000396          203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000396 DAZKAS010000000
VERSION     DAZKAS010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.denovo.410"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           73 a           28 c           38 g           64 t
ORIGIN      
        1 tatgcgtttt tttgttgatt taccaacatg tttagacatt ttcctctagg aaaaaattaa
       61 ctagcaccac gggtataaag gaaacggata acactttaca atgctgtttt ggtactccaa
      121 tttaaacaaa acaatcgaat ttacgtactt aattaagtac gttagaatgg agatataaat
      181 tagaggggga gtagatatgg caa
//
LOCUS       DAZKAS010000397        16971 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.048, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000397 DAZKAS010000000
VERSION     DAZKAS010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.048"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACG8H7_003057"
     CDS             complement(134..577)
                     /locus_tag="ACG8H7_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFP8985057.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACG8H7_003058"
     CDS             complement(795..2135)
                     /locus_tag="ACG8H7_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP8985058.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACG8H7_003059"
     CDS             2404..3300
                     /locus_tag="ACG8H7_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFP8985059.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACG8H7_003060"
     CDS             complement(3450..4391)
                     /locus_tag="ACG8H7_003060"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP8985060.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACG8H7_003061"
     CDS             4517..5638
                     /locus_tag="ACG8H7_003061"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP8985061.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8H7_003062"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACG8H7_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFP8985062.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACG8H7_003063"
     CDS             7465..7992
                     /locus_tag="ACG8H7_003063"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFP8985063.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ACG8H7_003064"
     CDS             8203..9264
                     /locus_tag="ACG8H7_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFP8985064.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ACG8H7_003065"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ACG8H7_003065"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP8985065.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ACG8H7_003066"
     CDS             10177..12159
                     /locus_tag="ACG8H7_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFP8985066.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG8H7_003067"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ACG8H7_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP8985067.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="ACG8H7_003068"
     CDS             12881..14305
                     /locus_tag="ACG8H7_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFP8985068.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="ACG8H7_003069"
     CDS             complement(14486..15259)
                     /locus_tag="ACG8H7_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP8985069.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="ACG8H7_003070"
     CDS             complement(15256..16161)
                     /locus_tag="ACG8H7_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP8985070.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="ACG8H7_003071"
     CDS             complement(16315..16635)
                     /locus_tag="ACG8H7_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFP8985071.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5480 a         3098 c         3480 g         4913 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa t
//
LOCUS       DAZKAS010000398        15360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.060, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000398 DAZKAS010000000
VERSION     DAZKAS010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.060"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..270
                     /locus_tag="ACG8H7_003072"
     CDS             <1..270
                     /locus_tag="ACG8H7_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFP8985072.1"
                     /translation="GKVINFNSIAKEANVSKSWLYKEHDIRQRIESLRERQITANVVS
                     KPKKSSRSEEILIKTLKRRVMELEKENKKLQNQIQKLYGDLYNKE"
     gene            424..1206
                     /locus_tag="ACG8H7_003073"
     CDS             424..1206
                     /locus_tag="ACG8H7_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFP8985073.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1332..2063)
                     /gene="erm(A)"
                     /locus_tag="ACG8H7_003074"
     CDS             complement(1332..2063)
                     /gene="erm(A)"
                     /locus_tag="ACG8H7_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFP8985074.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2121..2180)
                     /locus_tag="ACG8H7_003075"
     CDS             complement(2121..2180)
                     /locus_tag="ACG8H7_003075"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFP8985075.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2572..3234
                     /locus_tag="ACG8H7_003076"
     CDS             2572..3234
                     /locus_tag="ACG8H7_003076"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP8985076.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3469..3612
                     /locus_tag="ACG8H7_003077"
                     /pseudo
     CDS             3469..3612
                     /locus_tag="ACG8H7_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            3762..3962
                     /locus_tag="ACG8H7_003078"
     CDS             3762..3962
                     /locus_tag="ACG8H7_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP8985077.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(4216..5895)
                     /locus_tag="ACG8H7_003079"
     CDS             complement(4216..5895)
                     /locus_tag="ACG8H7_003079"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFP8985078.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(5897..6109)
                     /locus_tag="ACG8H7_003080"
     CDS             complement(5897..6109)
                     /locus_tag="ACG8H7_003080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFP8985079.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            6502..8232
                     /gene="efrA"
                     /locus_tag="ACG8H7_003081"
     CDS             6502..8232
                     /gene="efrA"
                     /locus_tag="ACG8H7_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFP8985080.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            8229..9989
                     /gene="efrB"
                     /locus_tag="ACG8H7_003082"
     CDS             8229..9989
                     /gene="efrB"
                     /locus_tag="ACG8H7_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFP8985081.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            10084..10281
                     /locus_tag="ACG8H7_003083"
     CDS             10084..10281
                     /locus_tag="ACG8H7_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985082.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            10434..10919
                     /locus_tag="ACG8H7_003084"
     CDS             10434..10919
                     /locus_tag="ACG8H7_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP8985083.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(11023..11616)
                     /gene="clpP"
                     /locus_tag="ACG8H7_003085"
     CDS             complement(11023..11616)
                     /gene="clpP"
                     /locus_tag="ACG8H7_003085"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP8985084.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            11796..12323
                     /locus_tag="ACG8H7_003086"
     CDS             11796..12323
                     /locus_tag="ACG8H7_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985085.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            12414..13151
                     /locus_tag="ACG8H7_003087"
     CDS             12414..13151
                     /locus_tag="ACG8H7_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP8985086.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            13155..14072
                     /gene="miaA"
                     /locus_tag="ACG8H7_003088"
     CDS             13155..14072
                     /gene="miaA"
                     /locus_tag="ACG8H7_003088"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP8985087.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            14074..15303
                     /gene="hflX"
                     /locus_tag="ACG8H7_003089"
     CDS             14074..15303
                     /gene="hflX"
                     /locus_tag="ACG8H7_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFP8985088.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         4998 a         2567 c         3198 g         4597 t
ORIGIN      
        1 ggtaaagtta ttaattttaa ttcaatagca aaggaagcta atgtttctaa atcatggctt
       61 tataaggaac acgatattag gcaaagaatc gaatcccttc gtgagcgtca aataacagca
      121 aatgtagtct caaaacccaa gaaaagttct cgttcggagg aaatccttat taaaacctta
      181 aaaagaagag taatggaatt agaaaaagaa aataaaaaat tacagaacca aattcaaaaa
      241 ttatatggag atctgtataa taaagaataa ttattaatct gtagacaaat tgtgaaagga
      301 tgtacttaaa cgctaacggt cagctttatt gaacagtcat ttaggtatat gtccaatcta
      361 gggtaagtaa attgagtatc aatataaact ttatatgaac ataatcagtt acgaggtgaa
      421 atcatgagca atttgattaa cggaaaaata ccaaatcaag cgattcaaac attaaaaatc
      481 gtaaaagatt tatttggaag ttcaatagtt ggagtatatc tatttggttc agcagtaaat
      541 ggtggtttac gcattaacag cgatgtagat gttctagtcg tcgtgaatca tagtttacct
      601 caattaactc gaaaaaaact aacagaaaga ctaatgacta tatcaggaaa gattggaaat
      661 acggattctg ttagaccact tgaagttacg gttataaata ggagtgaagt tgtcccttgg
      721 caatatcctc caaaaagaga atttatatac ggtgagtggc tcaggggtga atttgagaat
      781 ggacaaattc aggaaccaag ctatgatcct gatttggcta ttgttttagc acaagcaaga
      841 aagaatagta tttctctatt tggtcctgat tcttcaagta tacttgtctc cgtacctttg
      901 acagatattc gaagagcaat taaggattct ttgccagaac taattgaggg gataaaaggt
      961 gatgagcgta atgtaatttt aaccctagct cgaatgtggc aaacagtgac tactggtgaa
     1021 attacctcga aagatgtcgc tgcagaatgg gctatacctc ttttacctaa agagcatgta
     1081 actttactgg atatagctag aaaaggctat cggggagagt gtgatgataa gtgggaagga
     1141 ctatattcaa aggtgaaagc actcgttaag tatatgaaaa attctataga aacttctctc
     1201 aattaggcta attttattgc aataacaggt gcttactttt aaaactactg atttattgat
     1261 aaatattgaa caatttttgg gaagaataaa gcgtcctctt gtgaaattag agaacgcttt
     1321 attactttaa tttagtgaaa caatttgtaa ctattgaaaa tagaaagaaa ttgttccttc
     1381 gatagtttat taatattagt gacatttgca tgcttcaaag cctgtcggaa ttggttttta
     1441 gtgaaaagaa cacgatattc acggtttacc cacttataaa caaaagatcg atactttttg
     1501 tagtccttct ttgaaatcaa tggttgatgt cgttcaagaa caatcaatac agagtctaca
     1561 cttggcttag gatgaaaata tagtggtggt acttttttga gcatttttat atccatctcc
     1621 accattaata gtaaacccaa agctcgttgc agattttgca atcttttcgc aaatcccttc
     1681 tcaacgataa gatagctata tttagcctga ctttcaaagg taattctttt gacaatatcc
     1741 gtactgatgt tataaggaat attaccatat atcttatagt ttatatgttt tgggaaggaa
     1801 aattttagaa tatccgtttg aatcactttt atattctcag aggggtttac cgcttcttta
     1861 gtcacttgac ataagcctcc atcaatttct atagcagtaa ctgatcgact cattttgact
     1921 agctctttgg taaaatgtcc ttttcctgat ccgatttcta ttacgttgtc ttgtttactg
     1981 atattcgtgt gattcaatat ttcttttaca tgctttttag aagtaataaa attttgcgtg
     2041 tctttagggt ttttctggtt cattataacc ttctccttac tggttataat gaactgacat
     2101 taagcgttca ttataaccaa ttaattttga tttggttgat aacgaacttt attaataaca
     2161 aatatagaaa aagtacccat acttttatct cctttccatt tttttcatta tgaatgagat
     2221 aaagtaattt ctactactgc gatactggtg cacataattg aaacctcctt atgtaactga
     2281 atatgattgt atcatctttt cattttagaa taaaccttta tatgtaattt tgcacttgac
     2341 acttcagatg gacgttttct tcaaaataat atgttttgaa acttgataag agaagccact
     2401 gtctttacta aaaagtaaag gctgcgataa aggacagtga ctttaataaa gttgcactgt
     2461 tgtattgtct ttggataaaa ttgtctaaag ggaagtttta tccccaacga aaaaacatct
     2521 aaagtggtaa gtaatgaaag aatgtcgata aagtaggaat gggggatctt tttgaataca
     2581 atagaatcac agtttgataa ggttgcagaa gattacgatt tcgtgaatga gcttttgaat
     2641 gattattcat tctttgtgtc taatatgtct ccaaagaaag gcagagcatt agatatcgga
     2701 tgtggctcgg gtttgttagt ggagaaatta gcaagttatt atgatgaagt ggtagggatt
     2761 gatatttcta atcaaatgct cgatcttgcc aaatctaaac gtcaactaac aaatacggtc
     2821 tatctgaata tgaatgcaga acaacttaat tttaatgaga agtttgattt tattgtaagc
     2881 cgaacaacct ttcatcattt ggatgatata gccagcgtaa tacagcaaat gaaggaactg
     2941 ttgaatgagg aaggaagaat agttattctt gataatgtat ctgaagtcga gacaccgcct
     3001 acctatgttt ataaattagg ggcaatccaa gagtttttac cgcattgttt caaatttgga
     3061 ataaaaaatg cgattagaat ttataatcac aacacatcta aatcatggct tgagcattta
     3121 gcttcagata agtacctatc tgaacaaaat tattacgatt tatatgaaaa gttgttgcct
     3181 ggatgccaat tccataaaat gggttgggca atgggggttg tctggacaaa ataacttaaa
     3241 gctgtggctt tgaaaagttg aatttgtgat tgttttcaga gctatcgggt gctttagtat
     3301 aaataacaat gaataaaaaa tcagcctgta tcttatgtta ctattaagta atttaagata
     3361 caggttttac tatttttgaa ataagtacct tccacttcaa atacatgtat tcaaagtgaa
     3421 agtattgatt taaatgattc ttttgtaatt tactcttgat aatatgaaat tccatcgcag
     3481 caggatatga attttaccaa aaggatacaa aagtgtggag aaatgatggg gatcactgta
     3541 ctggaccact tgatcatcgg acgcaaacga tattttagtt tgagagaaga aggaatgatg
     3601 gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat tatattgtaa
     3661 ttttgtttgg gaatagaagt tgaaacatct gttgcacctc tatcgtacac gagcaatgtt
     3721 acaatttaaa ttagtgcttt tggcacgagg aggatattat tatggaaaac ggaacagtaa
     3781 aatggttcaa cgctgaaaaa ggatttggat ttatctcacg tgaagatgga agcgacgtat
     3841 tcgttcactt ctcagctatc caaggtgacg gattcaaaac attagaagaa ggtcaagcag
     3901 tgactttcga cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt gtaaaagctt
     3961 aattttatct gtaaatcaaa ttgatggtag acatcaattt ttacgagaga acaaagcgag
     4021 taacaaaaat acctgccaca agagacaatt tctcttgtgg caggtatttt tttgtatgaa
     4081 aaagtaaata aatttaagag gctatgaaag agccaccttg tatcaaggaa taagaacaaa
     4141 caaaaatggc ttctcatgtt tttcatttgt tttctcttat attcaggaca aggtgctgat
     4201 ttcataacct cttatttatt cttcatctgg tgtcattacc attggcaaaa tcattggatg
     4261 tctttctgta cgttcgaata agaaaggctg aagcgcgcca gtcatcgctt ctttcaattt
     4321 atattctgaa cagtcaggtc cattcaaaca ttctcttaaa gcgtggaata agattcgctg
     4381 agcttcattg atcatttctc ctgattctcg catgtagaca aatccacgag agagaatatc
     4441 tggaccagcc aagatttctt ttttcttgat atccactgtc gcgacagcta atacaagacc
     4501 ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta ccaatatcac cgatcccgct
     4561 accatcgata tacacatctc cagccacaaa atgcccagca ggacgcgcag aatcgctagt
     4621 taacgctaaa acatcgccat tttccatgat gaagcagttt tctgcaggaa cacctacatc
     4681 ttgtgctaga gaagcatgta ctttcaacat gcggaattct ccgtggacag gaataaaata
     4741 ttttggcttc atcaaacgaa gcattaattt ttgttcttct tgtccaccgt gaccggaagt
     4801 atggatgtta ttgatttttc cgtgaatcac ttctgcacca gcttcagaca gcaaattgat
     4861 caaatgattg acacttgttg tattaccagg aataggtgaa cttgaaaaga tcactgtatc
     4921 gtctggctgg atttggattt gacggtgagt accattcgcg atgcggctca acgcggccat
     4981 cgcttctcct tgagaacctg tacataggat catcgtttca ttggcaggca agcgattgat
     5041 ttcgtgtgca tctacaaatg tgccgtctgg aactttgata tatcctaaac gttgaccgtt
     5101 gacgatggca ttttccatgc ttcgtccgaa aacagcgatt ttacgaccag ttgccacagc
     5161 ggcatctgct gcttgctgca aacggaagat gttagaagca aagctggcaa aaatgatacg
     5221 tccttcgatt tttgaaaaga tcttcaaaat agaatgccca atcgtttttt ctgatttagt
     5281 aaatgttgga atctcagcat ttgtactatc agataaaagg cacaagactc cttcttcccc
     5341 gatacgagcc attttatgca agtttgctgg ttcgccaaca ggagtgaaat cgaatttgaa
     5401 gtctccagtc gctacgatat ttccagaagg tgttttcacc acgatcccta atgcatctgg
     5461 gatactatgg gtcgttctga aaaagctgac agaagtcttt ctaaaacgga tgatcgtatc
     5521 ttcattgatc tcatggagct ctgcatctcg aagcaagcca tgctcatcta atttgtttgt
     5581 gatcaacgct aatgcgagag gaccagcata gataggaata tttgcttgtt tcagcaagta
     5641 gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc accaaggctt ttactttgtg
     5701 taagttttgt atgatgtagc tgtagtcggg aatgacatag tcaattccaa gtaaatcgtc
     5761 ttccgggaat ttgatcccgg catcaatcaa aatgatttca tcttggaatt gtaccccata
     5821 cgtatttttc ccgatttcac ctaatccacc aatagcaaag acgcctgtct cgttattttt
     5881 gatggaaagt ttcatattaa aactccacta atgagaaatc agcattttct ttttcgtact
     5941 ctagatgact tccttctaga agttgaacga attcaatatt gtatggggta ttttcttcca
     6001 cacgttggcg agcgtctaca tcgctttttg cctcaatgta aagagatttt gtttcttccc
     6061 gtttagggtt gcgtactttt gtttcttgat aataaacttt atagatcatt gttttgtctc
     6121 ctttatctgc gtaaagcagt gatttagcta gttgattttt ttagacaaca caaaacgagg
     6181 ggacaagcca cctggttttt catatgcttt gactagaata ctggattcga gtcaaagaga
     6241 caagcctcgt ttttgcttat ctgttcggat attttttcaa ccgtaccttc tgtataaaaa
     6301 atatacaaca attaattgtt attctagcat aaaacagcaa ataagtatag aaatcaacta
     6361 gattgaaaga aaaaatcaat gtttatcatc ccgctataat cataatgact tttttatgag
     6421 agaagtgttc tattaatagg ctcgttgtgc tatcattttt agtaatgaaa atgtaacaaa
     6481 agattaaaag gagatgagaa agtgaaacta atgtggcatt ataccatgcg ttataagaaa
     6541 ttcttgtttt tcaatttcat atgcgtattt ggttttattt tgattgaatt aggacttcct
     6601 acgattttag cacgtatgat cgatgtagga atccgcaatg atgattttga ttatgtgaag
     6661 caacaaggga tatttatgat cgttattact gtaatcggta ttattttgaa tatcttgtta
     6721 ggttattttg gttcgaggat tacgactaat atcgtagcag atattcgaga tgatttgttc
     6781 gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc taggcgtatc ttcattgatc
     6841 actcgtacaa caaacgatgc ttatcagatc atgttgttcc tgcaaaatat tttacgtatc
     6901 ggttttatgg caccaatgat gttcattgtc agtttgtaca tggtcatgcg tacaagtgtg
     6961 acactcagtt tgtatgtagt aggtgcatta cctttactat tattggcagt cgtagcgatt
     7021 gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa acttagataa aatcaacagt
     7081 atcttgcggg aaaatctttc aggattgcgt gtgattcgtg cattcgtcaa tgaaaagttt
     7141 gaagaaaaac gttttgctgt agtcaatgag aattatgctt ctagttcaaa aagcttattc
     7201 cgtttgatgg ctgttgcaca accgggtttt tatttcttat ttaatatcgt aatggtattg
     7261 atcatctgga gcggggcagt gcaaatcgat gcaggcgagc ttcgtgtagg agatttgatt
     7321 gcgtttatcg agtatatatt ccatgccttg ttctcattca tgttatttgc tagtgtgttc
     7381 atgatgtatc caagagcagc tgtttcagct ggacgtatcc aagaagccct agatgccaaa
     7441 ccttcgattg tagaagatcc aaatggtata tctgatacaa aaactaaagg gtatatcgaa
     7501 tttcaaaatg taacatttgc ttatccaggc cattcgcaag agccagtgat acgaaatgtc
     7561 agttttacag cctctccagg agagaccgtt gcttttattg gaagtaccgg tagcggaaaa
     7621 tctacgttga tccaattgat ccctcgcttt tatgatgtga gtgaaggaaa gatcttgatc
     7681 gatggagtag acgtgagaga ttatcggcta agcaagttgc gagataagat tgggtttatt
     7741 ccgcaaaaag cgctgctatt tactggaacg attgcagata atttgcgata cgggaaagaa
     7801 gatgctacgc aagaagaaat ggaacgtgca gctgagattg ctcaagcttc agaattcatt
     7861 tctcgaaaac cagatggatt taatgaacat ttatcagaag gtggttccaa tttttcaggt
     7921 ggacaaaaac aacgattggc aatagccaga gccatcatac gccgtccgga aatctatatt
     7981 tttgatgaca gcttttctgc attagattat caaacggatg cgaagttacg tgctcgtctg
     8041 aaaaaagaaa cgacagaatc tactgtattg atcgtagcac aacgtgtggg aacgatcatg
     8101 catgcagata agatcatcgt attaaatgaa ggagaagtcg tcggtatggg gacgcatcgg
     8161 gagttgttgg aaaaatgccc gatttattat gatattgccg cttctcaatt atcaaaggag
     8221 gaattagcat gaaaaaaacc ttttcttcct taaaacgttt agctcgatat atcagaccgt
     8281 atcgtgagac ttttctttta gtgatcctat ttacagttct gacagtggct ttcaacgtgg
     8341 ctttgccata tgttacagga cttcctacta cagaaatcag ccgtaatatt gccaatggcg
     8401 aatcaatcaa tttttcttat atattcaaat gtctcatttg gatcacagtt gttggaatag
     8461 ggtattgtat ttcgcaatta ctttctggtg ttttgatgac aaatgtcgtt caaagtgcca
     8521 tgcatgattt gagaagagac atagaagaaa aaatcaatcg tcttccggtt tcatatttcg
     8581 ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa tgatgtagat gcagtcagcg
     8641 gtgctttaca acaagctttt attggtatcg taaatgcgat attgggaatt acgttggctg
     8701 cagcaatgat gttttatatc cagccgatga tggcgctgat ctctatgatc atgattccaa
     8761 catcgatctg gatctcaaaa cgagtgatca atgcttcgca gaaatatttt caaagcatgc
     8821 agaattctct tggtgagcta aacggctacg tccaagaaaa tatgacaggt tttagtgtat
     8881 tgaaagtata tggacgcgaa aaagaaacat tggaaggatt caaaaaagtt aatcacagct
     8941 tgaaatactt tgggtttcgt gcagcattca tttctggctt gatgatgcct ctagttcaat
     9001 taactgccta tgctacttat attggtatgg ctgttctagg aagcttttat gttattactg
     9061 gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata tatttggcaa atcagtcagc
     9121 caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag tgcatcagcg gcaacgaaac
     9181 gtgtattcga gattttagat gagccagaag aaaaagaaaa caaagtagat gcttcactgc
     9241 cagaaacgat tttaggagat gtgacattcg atcatgtcga ttttgcatat gatccgaaga
     9301 aaccattaat ccaagatttg aattttgaag tgaaagctgg tcaaaccgta gctgtggtcg
     9361 gtccgaccgg tgccggcaag acgacattga tcaatctatt gatgcgtttt tatgacgtag
     9421 acaaaggagc aataaggatt gatggaatcg acaccaaatc aatgagtcgc agtgatgtgc
     9481 gttcgctatt tggtatggtc ttgcaagatg catggcttta tcaaggaacg attgcagata
     9541 atatccgctt cggaaagctt gatgcgacag attatgaagt agttgatgct gcgaaaacag
     9601 caaacgtaga tcattttatc cgaacgatgc cagatggata tgatatggcc atcaattctg
     9661 agggagacaa tgtgtcttta ggacaaaaac agttgctgac gattgcccga gcagtggtct
     9721 ccgatcctaa aatcttgatt ttagatgaag caacaagttc agtagacacc cgtttagaag
     9781 cgttgattca aaaagcaatg gatcgcgtga tggagggacg gacaagtttc gtcatcgctc
     9841 accgtttatc gacaatccgt gatgcagatc tgattctagt gatggatcaa ggtcagatca
     9901 ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg attttatgaa aaactgtata
     9961 acagccaatt tgctgaggaa gcagaataag tcatataaga ataaaacagt ttaaaactta
    10021 ttttttgttc tctattcttg ataatggtaa agtaaagtta tcaagaaaag tgaggacacg
    10081 gagatggaac agtttttaaa tgaaaaaatt actgaagcca aaagagaagt tgatcgttta
    10141 acgcacgtct gtcagacgaa gccttttgat tcagctgatt tttttgaaaa tgagtttcaa
    10201 atcgaacgct tggcagcaca aatagaagtg tacgaattaa ttttaaaaaa agtaatagaa
    10261 gaaaatatag atgtacaata aaaacaggta aaacgtcagt taccagatac tgtaaataga
    10321 catctggcct gagcaggttt tacctgtttt ttttcttatg aaataaatat atgctatgat
    10381 acctttattc aataggatta gtttctacaa aaaatgaaag gagaaaaata aatatggaac
    10441 ggtttatagc atcaaatgcc acagtgattg gtgatgtgac attgtcagaa gatgtgacga
    10501 tctggtatca agctgtttta cgcggggaca gtaattggat aaaaatagga caaagaacga
    10561 atatccaaga tggtacaatc atccatgtcg atcatgatgc acctgtagat attgcggaga
    10621 atgtgacggt aggacatcag tgcatgctac atggctgtac aatcgaaaaa ggggcgctga
    10681 tcgggatggg gaccacgata ttgaatcatg cggttattgg tgagaatagt ttgattggtg
    10741 ctggatcatt agtaacagaa ggaaaagtta ttcctcctaa tgtcttggct tttggtcgcc
    10801 cagctaaagt gatccgtcct ttgacaaaag aagaaataca aaaaaacaaa gaaaacatcc
    10861 agcattatgt cgagataggt cggatgcatg cgcaaggaaa attcaaggag caaagatagg
    10921 cagtagcatc cataaaataa aaaaggatgg tcagtttgag ttgaccatcc ttttttattt
    10981 ggtttagata agagagctta aaaaagcttc ctttgaaaca gattaattta atgaagaact
    11041 gttttccatg acttcatcga tcaatccata tgcttttgct tgttcagctg tcatatagtt
    11101 gtcacgatct gtatcttttt caatcacttc aagcggttgt ccagtgcgtt cagctaaaat
    11161 tttgtttaag cgttcgcgtg tttgcaaaat atggcgtgcc gcaatttcga tttcagttgc
    11221 ttgcccttga gcgccgccaa gaggttgatg gatcatgatc tcagcatttg gtaaagcaaa
    11281 tcgtttacct tttgtaccag ctgtcagcaa gaagcttccc attgaagcag ccatacccat
    11341 aacaattgtt tggacatctg ctttgacaaa gttcattgtg tcgtaaatag ccatacctgc
    11401 tgtaactgaa ccaccaggag aattgatata aagatagata tctttttcag aatcttgagc
    11461 gtctaagaaa agcaattggg cgatgatcga attagcaaga tcatctgtta cttggccgct
    11521 taacatgatg atacggtctt tcaataaacg tgagtagata tcgtaagctc tttcacctcg
    11581 tgaagattgt tcaatgactg taggaattaa attcataata gagttcctcc ttgatttcgt
    11641 ttagaaatag tgtaccataa tttagtttct aatacctaaa atgtagtata catttttggt
    11701 caaaaaaggt caaataaaaa acttaggcaa attagtcgca taaacccgct ttatggtatg
    11761 ataagcagag aaacaaaaca aggaggaaca acagattgat agttggtatt actggcctta
    11821 tcggagtgct gacaggtgct gtagttgtct atatcggcat gatactgcaa atgcgtcact
    11881 tggaaaataa agaacagcgt agacgtgaat tggaattgaa agtcaaagaa atcgagacgc
    11941 tgaatcaatt aaataaaaag atcaacgaga tcttgcaaaa acggacaacg ctgatggaag
    12001 aatatgtcag ctttgatgca tttgatgatt gttatatcac gattgacgat tttgcgtatc
    12061 tccaatcatt ttcagcgcaa aatagttttt atctgccaaa ttatatttta gacgagctat
    12121 ttaagaatat ctcccatcga aaagtgattt tatctccaga tgaaacaatg aagattggtg
    12181 gatatgctta taaaggcgga cgcgtgattt tagagaattt tacggatagt ctgactgaaa
    12241 tgatcaatga aaaaaaagct cagttgaaga ctatgacgcg ccagccgatt ggtttcttct
    12301 cgaaagatga tgtttggagc tgatattttg aagcattgcc gttgcacatg agagcgtggt
    12361 aatgctttct ttttttctta atgatacaat agaactgaac ggagtgaaga aagatgttac
    12421 aaatctatgc ccatcgcgga agcagcggca cccacccaga aaatacgctt cctgcatttg
    12481 cagaagctgt acgagtaggt gcagatggta tcgaactaga tgtccatcta agcaaagatg
    12541 gttatttgat cgttatgcac gacgaagaag tggaccggac gactaacggt aaagggctga
    12601 ttcgagaaaa aacattggaa gaactcaaaa aattgaatgc agggggcaaa ttcagcaaag
    12661 aatttccagc agctaaagtt cctgaattga gagaagtgtt aggactgctc ctccaaaaaa
    12721 attaccgagg gatgctaatg attgaaatta aaactgatca gtatgaatat cccggtatcg
    12781 aagaaaaatt agccgaaaca ttaaaaagta gagattggcc ttttcaacat gcatattgca
    12841 gctttcattt ggattctttg gaaaaaatcg cattactgga accggagata cagctggact
    12901 tattgatgag tacttctgat aaaaagatcc aactagcagc agaaacccct tatatagaag
    12961 gagtacatcc aaaaaatact tggttgttcg aacatccagt ccaggcagct gattttccaa
    13021 aagcaattcg tgcatggacg attaacgatg aagaagaaat ggccaaatgt caagaactca
    13081 aaatagatgg aataataact gattttccgg agaaagctct tcggtggaaa caatcaacga
    13141 aaagtaggta aaatatggaa aaagtattag tgatcgtcgg tccgacagca gtcggtaaaa
    13201 cagcgctaag tgtggaactc gcacaactat ttggaggaga gatcatcagt ggtgattcat
    13261 tgcaaatcta taaaaatctg gatatcggta cagcaaaaat caaagaagaa gagatgaata
    13321 atgttccgca tcatttgatt gatgtgatcg aacctactca acgttactca gccgctgatt
    13381 ttcagcattc aggtcgacaa ttgattaccg agattgcgaa ccgagggcac ctacctatca
    13441 tagctggtgg tacgggtctc tatatccaat cccttctata tgattatcag ttaggagcga
    13501 cagaagaagc gaatcctgag attagaaaaa agtatgaaac gttagctgaa acaattggtt
    13561 cccaagcatt atggaatcag ttgaaagaaa aagacccggc agctgctgag aaaatccact
    13621 ggaacaatca acgcaaagtg ataagagctc tggaagtttt agagacgaca ggacatagta
    13681 tcacggcacc taaagaagag ccaaagcaat tgtatgatta cctcatgatt ggattagata
    13741 cgaagcgtag tattctttat gaacgaatca atcaaagagt cgatttgatg ctagcagaag
    13801 gtttagtagc agaagcgcgt aaggtgtatg agcttggaga agtccaggcc agtcaaggta
    13861 ttggatataa agaatttttc ccttattttg aagggaaaga aacattggaa gaagcaacag
    13921 aacaagtcaa actacattcg agaagatacg cgaaacgtca gttgacgtgg tttcgtaacc
    13981 gtttggatgc acagtggttt gatcttataa gtgagccaaa tcaaaaaagt cagattgagc
    14041 aaacaatcaa aaaatggctg gaggcggaat aaaatggaag aaaaagtgat catcgtaggg
    14101 gtagaaaccg aagcaaacca gcgttacttt tcagagtcaa tggaagagct agttcagctg
    14161 acgaataccg catcaggaga agtcgtattc acaattacac aaaagagacc acaagtggat
    14221 cggcaaacga ttatcggtaa agggaaatta caagaattag ttcaacaagc ggatgcgcat
    14281 gaagcggatt tgatcatctt taatcatgag ctaactccga gacagagcca actgattacc
    14341 gatgctgttg gtcttccggt aattgatcgc gtgcagctta tcttagatat ttttgcgatg
    14401 cgtgctcgtt cgaaagaagg gaagctgcag gttgaacttg ctcagttaga atacctgctg
    14461 cctcgtttag tagggcaagg gaaaacatta tctcgtcttg gcggtgggat cggaacaaga
    14521 gggccaggtg aaacaaagtt agaaacagac cgccgacata tacggaataa aatttttgct
    14581 gttaagaaag aattgaaaga agtagaagct catcgagagc gcaatcgcca aaaaagaaaa
    14641 aatagcgaga tttttcagat tggacttatt ggctacacga atgccgggaa gtcaactatc
    14701 ttgaatctat tgacacaagc ggatacttat tcgaaagatc aattgtttgc aacgttagat
    14761 ccattgacta agagatggcg ttttgctgaa ggatttgaga ttactgtaac ggataccgtc
    14821 gggttcatcc aagacctgcc tacgcaactg atcgatgctt tccattctac attagaagaa
    14881 agtcaaaata tggatctttt gctacatgta gtcgatgcta gttctccaga tcgtatttta
    14941 caggagcaga ctgtcttaaa attgatggat gaactgaata tgaaagaaat gccgatactg
    15001 actgtttaca ataaggcaga ccagatcgac ccagcgatgt tcacgcctac gctattccca
    15061 aatgtattga tttctactca gacgaaagaa ggtaagatgg cgttgatcga agcaatcaaa
    15121 cgtcagctga tggaactaat gaccccttat acactttctg tgccaagcaa cgaaggacag
    15181 aaattaagcg agttgagaag acaaacaatg gttttggacg aagcgtacat ggaagaaaca
    15241 aacgaatatg aagttaaagg gtttgcaaaa accaattcaa aatggttaag aaaaaaccaa
    15301 taaaatttta tttttttaac aatattattt gtcaaattaa gcgagacaaa actttccatt
//
LOCUS       DAZKAS010000399         5806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.148, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000399 DAZKAS010000000
VERSION     DAZKAS010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.148"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            325..789
                     /locus_tag="ACG8H7_003090"
     CDS             325..789
                     /locus_tag="ACG8H7_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP8985089.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            773..1402
                     /locus_tag="ACG8H7_003091"
     CDS             773..1402
                     /locus_tag="ACG8H7_003091"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFP8985090.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1756..3234
                     /gene="msr(C)"
                     /locus_tag="ACG8H7_003092"
     CDS             1756..3234
                     /gene="msr(C)"
                     /locus_tag="ACG8H7_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFP8985091.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3281..3727
                     /locus_tag="ACG8H7_003093"
     CDS             3281..3727
                     /locus_tag="ACG8H7_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFP8985092.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3926..4441)
                     /locus_tag="ACG8H7_003094"
     CDS             complement(3926..4441)
                     /locus_tag="ACG8H7_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFP8985093.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4558..5760
                     /locus_tag="ACG8H7_003095"
     CDS             4558..5760
                     /locus_tag="ACG8H7_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP8985094.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1798 a          967 c         1230 g         1811 t
ORIGIN      
        1 attactgttt aagcatactg atccaatttc taatattcga cgggattctt gcaaaaggcg
       61 atttcggaaa tttttggcgg tgtaaaatta tctactgcag aagagctatc ttctatagtt
      121 tgataagaat caataatctt ggttttagcg ttgtgctttc tttatcattt tttccgttca
      181 ttctacaaaa aatttctttt ctctatactt ttctattgag agcgtttttt ttattacaca
      241 gctgattctt tgactttttt ctttttttat tctattattg acaagtcaat cgttgacgag
      301 tcaattatta gaaaggagga aattatgtct ttagaagaga ttagtcacct tttatatcaa
      361 atcaaattag ccgatcaaaa aatcgtccag atctttgaag aaaatatcgg tataagtctt
      421 actagatacg agatcatgat gagactgaaa gaagaaaatc cattgctaca aagcagtttg
      481 caagaaaagt tgcagattga tcaaggagca gttactcgtc atctgaaggt attggaagca
      541 aatggctatg tttcgcgtca gagaaatcct cagaacaacc gggaagtgta tgtagcgcta
      601 acagaaaaag cccagaagaa aatcaagcat tgtgaaatgg accataaaga tacaggttcc
      661 gtgctatccc cggctttcag cgatgtagaa atgaaacaat ttctaacact tttagataaa
      721 ttgaataccc aactttgcaa tataacaaca gaaacgaaag aggttaataa taatggaaca
      781 aaaaaatgat ttttcaacaa ttatgactgg tagaagatcc gttcgcgtat atgatgaaac
      841 atacaaaatc ccacacgaag aaatgctgga aatgatcaaa gaagcgacga ctgcaccttc
      901 ttctgtcaat atgaaaccat ggcgctttgt ggtcgctgaa agcgaggaag caaaagaaat
      961 cttgcgtcct ttgatccgtt tcaatacgag acaaaatgat acttcttctg caatggtcat
     1021 gatttttggc gatatgcaat gttacgaata tggcgaagaa atctacaacc aagcttacga
     1081 atcagggaag atgccaaaag aagtcagaga tcaacaatta gctgcgatca ttccttatta
     1141 caagagtttt tcaagagaag aaatgaacga tgtagtcaaa gtcgattcta gcttagcggc
     1201 tatgcaattc atgttagttg cgcgtgatca tggctatgaa acaaatccaa ttggtggatt
     1261 cgaagcagat cagttggcag aggcatttgg tttggataaa gaccgttatg ttccagttat
     1321 tattttatca ataggaaaag cggtagaaga aggttacgag tctgttcgtt tagcaccaga
     1381 aaagatcact atgttcgaat aaaaggtttt cagttactat tcagtagcta ttgctttttt
     1441 tgaatcgtgt gtggtaacct aataaaaaag gaggtcggaa aaatgactgc atcgatgaaa
     1501 ttacgtttcg aacttttgaa taacaactaa ataaattcaa aagacatgta taaacaacaa
     1561 tttcatggat ggagtatgtc cattttttcg aacgacagta gaataggtga aagctatgac
     1621 agatttggtg atttagtcat agttatttcg ttcagagtag gaaaagatgt gatgcagttt
     1681 ttttgcaaac agatttttct gctctttttt agtatcgata attgttgttt tacatgaaat
     1741 aggagtgtaa aacatatgga aaatttagca gtaaatataa caaatctaca agtcagcttt
     1801 ggcaaccagc tagaactatc tattgattct cttcgtgtct atcagcaaga tcgtataggg
     1861 atcataggag aaaatggtgt cggtaaatcg actttgctca aactaatagc cggtgaactt
     1921 tttcccgatc atgggaaaat ccaaacagag atcaccttca actacctgcc tcaattaacc
     1981 tatctcgctg aagcaaagga cctaaatttg gaattagcca gtcatttcca gttaagactg
     2041 gaagaaactt cagagcggaa atggagcgga ggggaagaac gaaagatcga gttgatacgt
     2101 cttctttctt cttatgaaca agggatgctt ctagacgagc caacaaccca tctagataga
     2161 aaaagtattg atcgactgat tgaagagctt cgttattatt atggtacgct ggtttttgtt
     2221 agtcatgatc gctattttct agatgagcta gcatcgaaaa tctgggaagt aaaagacgga
     2281 gaaatccgag agttttcggg gaattatagt gcctatctca ctcaaaagga attggagaaa
     2341 aagactcagc tacgagaagc agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg
     2401 atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag
     2461 caacaaatca gactggatcg gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc
     2521 atccaaaaga atgcgaaaac attagagaga agactccaaa aaataggaga aacaaccaag
     2581 ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact ccacagccgc
     2641 tatcccatca tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt actggtaaat
     2701 ggtgattttc agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt
     2761 aagacaacct tattagaaca tatccgcaaa caaggagaag gaatccttct ctctccgaaa
     2821 gtaagctttc aagtatatca gcaaaagggt tatcaaatga catctgaaga atccatcatt
     2881 cgttttgtca tgagacaaac agagttttca gaatcgcttg tccgtagttt gctgaatcac
     2941 ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg gggagaagcg
     3001 acccgtctga cgattgcttt gctttttact aagccaagta atgtgttgct gttagatgaa
     3061 ccgactaatt ttattgatat ggcaacgatc gaagctttag agaagctaat gcaaatatat
     3121 ccgggaacga ttttgtttac ttctcatgat tcttactttg tcgagcgtac ggctgatgaa
     3181 gtttatgaaa taaaagggca gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc
     3241 tacgatgatt ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa ataaaaaaga
     3301 aggaataatt tttacaacaa tgatgtgttt tttgatggtc ttggggatga gcgcgtataa
     3361 tttatggctg catcaagatc ttcatttatc aagtttgttg attggtctag tgcctggatt
     3421 cgtggtggct ttcattttgg atgtcttcat catcggtgtg ctggcgaaaa aaattgcctt
     3481 taaactacca gtaaatccag aaagcaaggt ccaaatgatc ttaactattt catgcttgat
     3541 ggttttagga atggtgacat gtatgtcttt attcggtgtg atcatggaac aaggaatacc
     3601 tgatcaatta ttctctgctt atatgaatgc ttggaaaatg aatgtgattg ccgctttacc
     3661 gttgcagttg atcatcgttg gcccgctttc aagaagtgtg ctaaaagcaa ttcaaagtaa
     3721 tgaatgatgg aacactgtta tttgaaaaaa ggccttggca tgctttttgt catagtctct
     3781 tgaatgcagt tctagaaaaa tagtgatccg aacgataggt tttggcctgt cgttcggatc
     3841 gctttttctt ttccaactgt attgctcttt tattgaagat aatttgctct tcaatagata
     3901 tttctagaaa ggttctgttt atcccttact caaaaaactg ctgcatttgt tctcttactt
     3961 gctgacacca ttggagttcc tgctgacaaa gcataattct gcgctggatc acaacataac
     4021 gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa ttttttttga tttttttgca
     4081 tgattttgtt cagctgttgt tctctagtga atagcagtcc agtagctgtt gttttatcga
     4141 gcgtcgaaaa agcatagatt tttgctagaa attcatcttt ttgcgtagga gcgggagttt
     4201 cctctttgat ccattcctct acaagagcac gtccttcatc agtcaagtgg tagatctttt
     4261 tttgagagtg ctcgtctttg tccaaaacgg tcacataacc ctgctggtct aacttggcaa
     4321 gcgttgtata gatctgactg tgatgggctt cccaaaaaat tgtgatcagc tgcttcaatt
     4381 catacccaga acgtggtttg gataaaagca cgcataataa tatataggct aaagtattca
     4441 taaaaatcct acctctcgtc ttattttctt tattatatca cagtaaagtt tccacgtttt
     4501 acatgtgcat attgacatgt aaaataaatc atgtatgatg ctaatagaga gaaagggatg
     4561 gaaatattgg agaatcaaat agatatacaa gaacaaacaa atgaagaaac aaaggggaaa
     4621 agttcagcgc tagttgtagc agctacctgt atgatcgctt ttatgggagt gggtctagta
     4681 gacccaatct taaaaacgat tgctgcacag ttaaacgcta cgccagctga aacgaccctg
     4741 ttatttacta gttacatgtt agtcactggt gtggtgatgc tatttacagg tttcttatct
     4801 agtcggatcg gtttgaaaaa aacattgatg acaggattgt ttatcatcgt tgtattcgct
     4861 ggtcttggcg gattatctgg gaatatcgga atgctgattg ggttacgtgc cggctggggt
     4921 gtcgggaatg ctttatttgt ctctactgct ttagcaacga tcgtcagtat tttagttggt
     4981 aacacagaaa aagcaatcat gatgtatgaa gcagcacttg gctgcggaat ggctgttggc
     5041 cctctagtcg gtggggtctt gggcagtggt tcatggcgtt atccattttt tggggtagca
     5101 tttttgatgt tttgcggttt tatagcccta gcggtacttt tacctgaaac tgagaaacca
     5161 aagagaaaag taggcctttg ggaaggtgtc aaagcgttaa gcaatcataa acttcgttca
     5221 gttggactaa tcgcattgtt atataatttc ggattcttta ctttacttgc gtatgcgcca
     5281 tttttattga ttggctattc tgaattagaa gtcggttttg tattttttgg ctggggtgtg
     5341 cttctggcta ttgcttcgat tttcgttgca ccggctcttg agcggaagtt tacgacttat
     5401 cggacaattt tagcagcatt tgtcttattc attatttgtt tgctgttatt aggagctgga
     5461 acttctatgc cagtccttgt accgatcagc attgtgttag caggattttt ccaaggaatt
     5521 atcaatacac tgttgacgac gattgctatg gaaataccag gtctgcaacg aaacgttgct
     5581 tcgtccagct atagttttgt ccgctttttc ggtggagctt tagcaccatt tattgcaggt
     5641 gtgattggtg aacatattgg tgaaagctat gcgttttatt ttgcaggtgc cattgttctt
     5701 atcagtgtat gttgtatcat ttatcaccgt aattattttg ctacaaatga agaagaataa
     5761 gaaatcagag gttgtgaaaa agctgtcctg catcaagtca taagaa
//
LOCUS       DAZKAS010000400         5561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.157, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000400 DAZKAS010000000
VERSION     DAZKAS010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.157"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..735
                     /locus_tag="ACG8H7_003096"
     CDS             382..735
                     /locus_tag="ACG8H7_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP8985095.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(793..981)
                     /locus_tag="ACG8H7_003097"
     CDS             complement(793..981)
                     /locus_tag="ACG8H7_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFP8985096.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1079..2206)
                     /locus_tag="ACG8H7_003098"
                     /pseudo
     CDS             complement(1079..2206)
                     /locus_tag="ACG8H7_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            2481..2978
                     /gene="dfrG"
                     /locus_tag="ACG8H7_003099"
     CDS             2481..2978
                     /gene="dfrG"
                     /locus_tag="ACG8H7_003099"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFP8985097.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(3050..5002)
                     /locus_tag="ACG8H7_003100"
     CDS             complement(3050..5002)
                     /locus_tag="ACG8H7_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFP8985098.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(5009..5245)
                     /locus_tag="ACG8H7_003101"
     CDS             complement(5009..5245)
                     /locus_tag="ACG8H7_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985099.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1754 a          975 c          904 g         1928 t
ORIGIN      
        1 cagattgttt tctctgttca tatgctttga tttttctatc tatgtaatct gcaaaaccac
       61 attgatggtc atatctatcc agtacaggtg gataagtttt tttgataaga acttaattaa
      121 gagaactaga tgacagatta tctttctcat ggcgactttt ccctccatta agtcgccaat
      181 tttatctact ttctaggagt atgactaaat tttataaagt tgtataggta aataaaatca
      241 gcgacctttc tatttgaaat tggattcatc gcattaataa attccacagt gttctatagg
      301 aactcataaa ctatataaca tgtttttcta tacctgttta tggttatata ggaacagtaa
      361 aatgtataga ggtgatttag tatgcgtaaa aaagaagata aatacgattt tagagccgtt
      421 ggtttagcga ttaaagaggc tcgaatgaaa cggggtctca ctcgtgaaca agtgggaaca
      481 atgattgaaa ttgacccacg ttatttaaca aatatagaaa acaaaggaca acacccaagt
      541 acacaagtgc tgtatgatct tgtatcatta ctccatgtat ctattgatga atttttctta
      601 cctacagata atttgattaa aagcactcga aggttacagg tagaaaaata catggatagc
      661 tttacagaca aagaactatc cttaatggaa gcactagcaa aaggtatcaa tgaagcaaga
      721 aatattgaag gctagtcagt aaaattcaga taaacaaaaa gagccgataa gatgagagtt
      781 tcatttctca aattatcggc tctgcgtctt ggcgtctggc tctttgattg ttattatatt
      841 cattttttga acattaatta aagtttcgtt cttacatttg gggcagtata aagggaaatt
      901 tttaagtata gtatccactc gtattcgtag tcttgtttta tttccacaaa taggacacaa
      961 tatccacttg tagtttataa taacaatctc ctcctttcca ctttaattca aatctatatt
     1021 aaagaatatt tcatcttatt taataagaaa ccatatttat ataacaacat aaaacgcact
     1081 aagttatttt attgaacata tatcttactt tatctatccg actatttaga cgacgggtct
     1141 ggcaaacagg ttcgccagtg gtaacctgat atccttttag ctctgctaaa caaacactaa
     1201 gcccatttgt aaaaaaagtt aaatcattgc gataatcttg aatacatcga gcaggaattt
     1261 ctccaataat aatgacctca ttatttttca gttgagtatt tacgatattt gcacaatatt
     1321 tgggagcatc gttatatgcc cgtgaaagat attcctgtgg tgcataaact ttaaaactaa
     1381 gatatggctc taacaattct gttccagctt ttctaaaggc ttgctccagt acaataggag
     1441 taagcatccg aaaatctgct ggagtactaa cagggctata gtataaaccg tacttaaaac
     1501 agattttaca atccgtcaca ttccaaccat ataatccttg ttcgcaacca tagcgtatcc
     1561 cttccataac tgcattttga aatgattgat ttaagtatcc aagagaaacc gagctctcat
     1621 actgcattcc acttcccaac ggaagcggtg atacagataa accaatggaa gcccagaaag
     1681 gatttggcgg cacttcgatg tgaatggtat attctgcatt ttttaacggt ctctccatat
     1741 aaatgactgt aggctctttt agttctatct ccacatgata cttttcttgc aacagtgcac
     1801 taatcacttc catttgtact ttccctaaga aagaaagtat aatttcatgt gtcgtagaat
     1861 ccacgtaata tcgtagaagc ggatcactat ctgagatttc caaaagggca tcaagcaaca
     1921 tttctctctg ttcaggttta ctcggttcaa cagttgtttg tagtagaggg tgcggatttt
     1981 caatcttttt tctctgtggc aatagttttg tatctccaag aacactattt aacttcaaaa
     2041 actcattttg caaaataaca atttctccag aataagctct atcaatctta cataattcac
     2101 catttattga agtatacatt tctgtaactt ttattttttc tttttctgat actctaaccg
     2161 aatctcgtaa atgtagtact ccactataaa ggcgtatata tgcaagtaag tagttcagtt
     2221 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     2281 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     2341 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     2401 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     2461 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     2521 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     2581 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     2641 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     2701 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     2761 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     2821 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     2881 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     2941 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     3001 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     3061 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     3121 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     3181 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     3241 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     3301 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     3361 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     3421 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     3481 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     3541 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     3601 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     3661 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     3721 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     3781 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     3841 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     3901 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     3961 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     4021 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     4081 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     4141 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     4201 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     4261 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     4321 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     4381 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     4441 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     4501 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     4561 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     4621 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     4681 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     4741 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     4801 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     4861 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     4921 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     4981 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     5041 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     5101 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     5161 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     5221 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     5281 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     5341 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     5401 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     5461 tactccaaaa ctgaactact taatatgcaa gacgttgtct tttttttgta tattcaattt
     5521 tgaaaacatt tccgcaaagt tcagacggac ctcgatgtgt t
//
LOCUS       DAZKAS010000401         3342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.206, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000401 DAZKAS010000000
VERSION     DAZKAS010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.206"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..734
                     /locus_tag="ACG8H7_003102"
     CDS             <1..734
                     /locus_tag="ACG8H7_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP8985100.1"
                     /translation="LFDSRFKTLQQNRKNDVVMYLAHDIKTPLTSIIGYLSLLDEAPD
                     MPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQMTDEF
                     YPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITAGLSGD
                     VVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYA
                     ESNDNYTTFRVELPAMPDLVDKRRS"
     gene            949..1917
                     /locus_tag="ACG8H7_003103"
     CDS             949..1917
                     /locus_tag="ACG8H7_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFP8985101.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1910..2941
                     /gene="vanA"
                     /locus_tag="ACG8H7_003104"
     CDS             1910..2941
                     /gene="vanA"
                     /locus_tag="ACG8H7_003104"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFP8985102.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2947..3312
                     /locus_tag="ACG8H7_003105"
                     /pseudo
     CDS             2947..3312
                     /locus_tag="ACG8H7_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT          996 a          653 c          810 g          883 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg aaaaaatgac gttgttatgt
       61 acttggcgca cgatattaaa acgcccctta catccattat cggttatttg agcctgcttg
      121 acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc acgttggaca
      181 aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg tataacctac
      241 aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg cagatgaccg
      301 atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac gcccccgagg
      361 atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac attttgaaaa
      421 acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc ctctccgggg
      481 atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag ctagctgcca
      541 tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt ggcgcgggac
      601 ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt tacgcggaaa
      661 gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac ttggttgata
      721 aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt atctttactt
      781 tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac ggtagactta
      841 ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga ttaccattgg
      901 catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat gaataacatc
      961 ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc tctttcgcct
     1021 cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa cgccaaatcc
     1081 gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc ctctattctt
     1141 cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg ctgcaatcat
     1201 atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc gtactcgccg
     1261 gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa cgtaaaatcg
     1321 attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg caaggtactc
     1381 agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt tattgagcgg
     1441 ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat agaggtaaac
     1501 tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca tgtgccgctc
     1561 aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa gcaaggagca
     1621 tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt taaagcatta
     1681 gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga agagtttttc
     1741 tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact tcaaagaatg
     1801 cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt gcgtgatacc
     1861 gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca tgaatagaat
     1921 aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg taaaatctgc
     1981 aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca ttggaattac
     2041 gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa acgacaattg
     2101 ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta aaaagaacca
     2161 tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca agtcaggtga
     2221 agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag gctgcgatat
     2281 tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga aaaatgctgg
     2341 gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg cagctacgtt
     2401 tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg tgaaaaaagt
     2461 caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg acagcaaaat
     2521 cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg gaaacagtgc
     2581 cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct ttcgtattca
     2641 tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc ccgcagacct
     2701 ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata aagcgctcgg
     2761 ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca ttgtactgaa
     2821 cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta tgatggccgc
     2881 tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt taaaggggtg
     2941 ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt tcgttgggac
     3001 gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta tgaagtaaat
     3061 cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga actggctgct
     3121 acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc tgtaaactgt
     3181 tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta ttatcccaat
     3241 attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag ccatagccgc
     3301 ggcggttctg ttgcaaagtt ttaaataaag aataaaatcc ct
//
LOCUS       DAZKAS010000402         2655 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.227, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000402 DAZKAS010000000
VERSION     DAZKAS010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.227"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..615)
                     /locus_tag="ACG8H7_003106"
     CDS             complement(<1..615)
                     /locus_tag="ACG8H7_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFP8985103.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYK"
     gene            708..1057
                     /locus_tag="ACG8H7_003107"
                     /pseudo
     CDS             708..1057
                     /locus_tag="ACG8H7_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1329..1619)
                     /locus_tag="ACG8H7_003108"
     CDS             complement(1329..1619)
                     /locus_tag="ACG8H7_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP8985104.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1827..2519)
                     /locus_tag="ACG8H7_003109"
     CDS             complement(1827..2519)
                     /locus_tag="ACG8H7_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP8985105.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          788 a          496 c          375 g          996 t
ORIGIN      
        1 cttataatta tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa
       61 aaaacaggcg ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca
      121 atatattttt ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag
      181 taaactaaat gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc
      241 cattactgtc aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt
      301 tattttctca aaaacatact tgcaaagtaa tactacaatt cctaacataa tcgtagaatt
      361 taataatttc catagcataa agtgtttaga aaagaacatt aagaatagtt ctataataat
      421 tcttgaactc catgtcttat aatgttctat cgaagcaccc aaatagccaa gttcatctaa
      481 tctacttgca taaactacgt catccgctct catagtcggc atatacatat taaagagtaa
      541 aaagataccc caaatcccta ttagtaacaa gatcagttct ttgttattcg ttatttttct
      601 attatgtttg ttcattttta tactccttct tatatttcct aatagagtat aacatagctt
      661 taagcattac ttaaagataa cttaaaagtt atcttttaaa tacttaattg ttaaatacct
      721 tatttgatgc aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat atccagaaaa
      781 atttttctta gttaaggacc tatggattgc agtaatgcag aattcgtcga ataaactgcc
      841 aaagacatac aatcatattg cttttaaaat tgacgaacaa gatatagatt cgtttgtctc
      901 aaaaatccaa atgttaggtt taactgtcga accaggacgt tctcgtgtga aaggcgaggc
      961 ttcttcaatt tacttttacg actatgataa tcacctcttc gagttacata ccggtactct
     1021 ccaggaacgt ttaaaatcct acaattccac tacctgagac tagtaatgac tagtctcttt
     1081 ttatggcctc tgatcccccc attgagcgaa tactaatagg gcttcgaatt gatctgttca
     1141 atctttaagc aatatccctc aaatgagttt ggaaagttta gtgattcatc acaaatactt
     1201 aaatacatgt agttatatat agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa
     1261 atcccatcgt ctgcatccat ctttccaaaa caagcggcta ggacattttt gtctcagccg
     1321 cttgttttct agtttattct ttcatcttgt cggacctttt ttcttgggta tagacttttc
     1381 tcatattcta ccacaatttt gggtttccct aacacatgat aggatctcac aacaaattgc
     1441 tttctattat tataataacc tgtaacgact aacttcatcc cttcgtctac ttcagctagg
     1501 aagttcaaac tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa acggatcaac
     1561 ggtctggcac taacttgcaa agtacggatc ttatctactg ttccatattt tttctccata
     1621 accttcacct caataccatt atacgaacat acattccctt tgtaaagaag aaactctgtt
     1681 ttatcttttt gactttttgt ctattacctt tcttaaaatc atattggcac ttcctgcgaa
     1741 gatgaaggac atgtactaaa aaaaagaggc agcagtatag attactgttg cttctttact
     1801 aatttaggtt tacttgttaa gaaagattat ttacgcaaat gttcttcaac aatttgctga
     1861 gatttatcag ctgtatcttc cacttcatct tttgcttttt ctaacttttg aatatattgt
     1921 ttcaagcttg agttttcagc agttgcttcg tctaatttct tttgtgtatc tgttaactga
     1981 gtttctacat ttgttttttc ttgtgtcagt gttgcgattt gttgattcaa accatttaca
     2041 gtattttgta attctgaaag ttttgcttgt gcttcttgta gtcttgctgt ccaatctgca
     2101 ttcagatgat tgatttcatc atttttttgg ttgatctcat ctagtttgac attgatttca
     2161 ttttgtttgc tttgtagttg tgcatccttg ctgactaatt gactttctaa gttttgtttt
     2221 tctgctgccg attgattttt ttggttttct aattcattta cttttgtttg gtattcttgg
     2281 acatctgcct ttgaatcttg caatgtctgt tctttttgtg ctagtcgagc ggtaagatct
     2341 gtaagcgctt gctctttatc tgtcaatgta tgatcaagct gtgtcagatt ctcattgatt
     2401 gtatcaatat ctgtctctcc accccacaaa ataatttcat ttgcgtaccg cgtgcctgtg
     2461 tagccaatta gacatagaaa tgcaacgaca atcccaccag taataaattt ctttttcatt
     2521 tgcatagctc cttaatccaa tacttttaca tgctaaaagt aagtgatctc aaacacaatg
     2581 tcaagcgttt ttaaattaca tgtgtaattt taatataaat actatcaaat actcatttaa
     2641 tcctcctttt ttatc
//
LOCUS       DAZKAS010000403         2355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.240, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000403 DAZKAS010000000
VERSION     DAZKAS010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.240"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1026)
                     /locus_tag="ACG8H7_003110"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="ACG8H7_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACG8H7_003111"
     CDS             1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACG8H7_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFP8985106.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..2318
                     /locus_tag="ACG8H7_003112"
                     /pseudo
     CDS             1851..2318
                     /locus_tag="ACG8H7_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
BASE COUNT          736 a          449 c          464 g          706 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatgg ttctgttgca aagttttaaa
     2341 taaagaataa aatcc
//
LOCUS       DAZKAS010000404         1781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.260, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000404 DAZKAS010000000
VERSION     DAZKAS010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.260"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACG8H7_003113"
     CDS             24..416
                     /locus_tag="ACG8H7_003113"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP8985107.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8H7_003114"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8H7_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFP8985108.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAZKAS010000405         1504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.274, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000405 DAZKAS010000000
VERSION     DAZKAS010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.274"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACG8H7_003115"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACG8H7_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACG8H7_003116"
     CDS             394..477
                     /locus_tag="ACG8H7_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFP8985109.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG8H7_003117"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG8H7_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFP8985110.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZKAS010000406         1382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.284, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000406 DAZKAS010000000
VERSION     DAZKAS010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.284"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1311
                     /locus_tag="ACG8H7_003118"
     CDS             <1..1311
                     /locus_tag="ACG8H7_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFP8985111.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAZKAS010000407         1350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.287, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000407 DAZKAS010000000
VERSION     DAZKAS010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.287"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACG8H7_003119"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACG8H7_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACG8H7_003120"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACG8H7_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFP8985112.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAZKAS010000408         1194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.303, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000408 DAZKAS010000000
VERSION     DAZKAS010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.303"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="ACG8H7_003121"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="ACG8H7_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFP8985113.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DAZKAS010000409          841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4518
            SAMEA115707171-rid22570303.guided.339, whole genome shotgun
            sequence.
ACCESSION   DAZKAS010000409 DAZKAS010000000
VERSION     DAZKAS010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707171
            Sequence Read Archive: ERR13196902
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287913.1
            Genome Coverage       :: 241x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:44:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,122
            CDSs (total)                      :: 3,062
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 60
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 31 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 27 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707171-rid22570303.guided.339"
                     /isolate="VRE4518"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..674
                     /gene="aac(6')"
                     /locus_tag="ACG8H7_003122"
     CDS             126..674
                     /gene="aac(6')"
                     /locus_tag="ACG8H7_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFP8985114.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          303 a          126 c          188 g          224 t
ORIGIN      
        1 attttataag taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt
       61 tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa
      121 gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg
      181 atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag
      241 aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat
      301 ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag
      361 aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag
      421 tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa
      481 cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc
      541 ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac
      601 caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac
      661 caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag
      721 gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct
      781 acttttggaa caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga
      841 a
//
